Exploiting the bacterial exopolysaccharide bioconversion using residual cheese whey as culture medium.

IF 2 4区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS
Elaine Cristina da Silva, Leandro Paes de Brito, Francisca Crislândia Oliveira Silva, Dayane da Silva Santos, Allyson Andrade Mendonça, Raquel Pedrosa Bezerra, Paulo Antônio Galindo Soares, Marcos Antônio de Morais Junior, Ana Lúcia Figueiredo Porto, Maria Taciana Holanda Cavalcanti
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Abstract

Cheese whey (CW) represents a potential substrate in biotechnological processes due to the presence of valuable nutrients in its composition. Therefore, CW is used as a low-cost substrate in fermentation for microbial growth and the synthesis of value-added compounds, while mitigating the environmental impact that this by-product can cause. The current study aimed to obtain exopolysaccharides (EPS) by fermenting lactic acid bacteria in CW followed by optimizing production using Response Surface Methodology (RSM) and evaluating the biological properties. Out of 64 isolates, Enterococcus sp. 133 V exhibited a high concentration with 5.58 mg/mL of EPS. With optimization using RSM, 21.74 mg/mL of EPS was obtained with temperature and fermentation time fixed at 42 °C and 22 h, respectively. The characterization of the new EPS revealed a hetero-polysaccharide consisting of galactose, glucose, mannose, arabinose, rhamnose and fucose, including proteins and uric acid in the structure. With a concentration of 2 mg/mL, the purified EPS showed good scavenging effects against DPPH (27%), ABTS (72%) and superoxide (43%), except for the hydroxyl radical (1.29%) which needs a high EPS concentration. These findings underscore the interest in using cheap residue as culture medium to produce biopolymers with potential for applications, particularly in the food and biotechnology sectors.

利用干酪乳清渣作为培养基,探索细菌胞外多糖的生物转化。
奶酪乳清(CW)由于其成分中存在有价值的营养物质,因此在生物技术过程中代表了潜在的底物。因此,CW被用作微生物生长和合成增值化合物的发酵的低成本底物,同时减轻了这种副产物可能造成的环境影响。本研究旨在通过连续发酵乳酸菌获得体外多糖(EPS),然后利用响应面法(RSM)优化产量并评价其生物学特性。在64株分离株中,肠球菌sp. 133 V的EPS浓度较高,为5.58 mg/mL。通过RSM优化,在42℃和22 h的发酵条件下,EPS的产率为21.74 mg/mL。对新EPS进行了表征,发现其为由半乳糖、葡萄糖、甘露糖、阿拉伯糖、鼠李糖和焦糖组成的杂多糖,结构中含有蛋白质和尿酸。纯化后的EPS在2 mg/mL浓度下对DPPH(27%)、ABTS(72%)和超氧化物(43%)均有良好的清除效果,但对羟基自由基(1.29%)的清除效果需要较高的EPS浓度。这些发现强调了使用廉价的残留物作为培养基来生产具有应用潜力的生物聚合物的兴趣,特别是在食品和生物技术部门。
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来源期刊
Preparative Biochemistry & Biotechnology
Preparative Biochemistry & Biotechnology 工程技术-生化研究方法
CiteScore
4.90
自引率
3.40%
发文量
98
审稿时长
2 months
期刊介绍: Preparative Biochemistry & Biotechnology is an international forum for rapid dissemination of high quality research results dealing with all aspects of preparative techniques in biochemistry, biotechnology and other life science disciplines.
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