Preparative Biochemistry & Biotechnology最新文献_第7页

Chitinase: purification and characterization from Alternaria brassicicola and its antibacterial activity. 几丁质酶的纯化、鉴定及其抑菌活性。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-09 DOI: 10.1080/10826068.2026.2697946

Nirja Thakur, Amarjit K Nath, Anjali Chauhan, Rakesh Gupta, Mahesh K Samota, Mukesh Choudhary

{"title":"Chitinase: purification and characterization from Alternaria brassicicola and its antibacterial activity.","authors":"Nirja Thakur, Amarjit K Nath, Anjali Chauhan, Rakesh Gupta, Mahesh K Samota, Mukesh Choudhary","doi":"10.1080/10826068.2026.2697946","DOIUrl":"https://doi.org/10.1080/10826068.2026.2697946","url":null,"abstract":"Chitinases, a class of enzymes found widely in organisms such as bacteria, yeasts, fungi, arthropods, actinomycetes, plants and humans, exhibit significant diversity in their molecular structure, catalytic mechanisms and substrate preferences. A newly characterized extracellular chitinase enzyme was purified from Alternaria brassicicola using methods including ammonium sulfate precipitation and gel filtration chromatography. The enzyme's molecular weight, determined via SDS-PAGE, was found to be 44 kDa. It displays optimal activity at a temperature of 35 °C and remains functional across a temperature range of 30-60 °C. The enzyme exhibits maximum activity at pH 6.6 and shows significant activity within a pH range of 4-8. Metal ion studies revealed that Cu2+, Ca2+ and Zn2+ inhibits its activity, while Mn2+ and Mg2+ enhances it. Furthermore, the enzyme demonstrates inhibitory activity against bacteria such as Staphylococcus aureus and Escherichia coli. These findings underscore its potential applications in various biotechnological and medical fields.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and modeling of ultrasound-assisted extraction of ursolic acid from selected medicinal plants. 超声辅助提取药用植物熊果酸的工艺优化与建模。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-07 DOI: 10.1080/10826068.2026.2697945

Sonali L, Sukumar M

{"title":"Optimization and modeling of ultrasound-assisted extraction of ursolic acid from selected medicinal plants.","authors":"Sonali L, Sukumar M","doi":"10.1080/10826068.2026.2697945","DOIUrl":"https://doi.org/10.1080/10826068.2026.2697945","url":null,"abstract":"The current research focuses on optimizing extraction parameters for isolating Ursolic acid from Plectranthus amboinicus (Indian balm), Rosmarinus officinalis (Rosemary leaves), and Ocimum basilicum (Basil leaves), using both maceration, a conventional technique as well as ultrasound-assisted extraction. A one-factor-at-a-time approach was employed to optimize extraction parameters. Among the three plant sources analyzed, rosemary exhibited the highest crude yield and ursolic acid content, and HPLC analysis confirmed the presence of ursolic acid with 26.956 mg/L in rosemary, 16.374 mg/L in basil, and 0.359 mg/L in Indian balm. Thus, rosemary was selected for further modeling and optimization to provide a basis for future scale-up studies, and mathematical models were applied to describe the effects of individual parameters, all showing good agreement of R2 > 0.96. RSM based optimization was further applied, which showed that ultrasonic power of 157.5 W at 45 °C for 40 min was the optimum condition with a maximum yield of 76.48%, with the model showing an R2 of 0.9990 and statistical significance (p < 0.0001). The optimized UAE process showed remarkable extraction efficiency compared to conventional maceration. Thus, this study, combining experimental optimization of extraction parameters, mathematical modeling, and RSM for efficient extraction of ursolic acid, is an integrated approach.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148397218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and optimal encapsulation of citral in proteins: effect of protein type, pH, and ratio. 柠檬醛在蛋白质中的制备和最佳包封：蛋白质类型、pH和比例的影响。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-06 DOI: 10.1080/10826068.2026.2696344

Tugba Dursun Capar, Demet Atici, Hasan Yalcin

引用次数: 0

Precision fermentation and recombinant proteins as enabling technologies for scalable cellular agriculture. 精密发酵和重组蛋白作为可扩展细胞农业的使能技术。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-06 DOI: 10.1080/10826068.2026.2693878

Neha K Jadhav, Aditya B Magdum, Kapil V Shinde, Mansingraj S Nimbalkar

{"title":"Precision fermentation and recombinant proteins as enabling technologies for scalable cellular agriculture.","authors":"Neha K Jadhav, Aditya B Magdum, Kapil V Shinde, Mansingraj S Nimbalkar","doi":"10.1080/10826068.2026.2693878","DOIUrl":"https://doi.org/10.1080/10826068.2026.2693878","url":null,"abstract":"Cellular agriculture has emerged as a promising strategy for producing animal-derived food components through controlled biological processes while reducing the environmental and ethical burdens associated with conventional livestock production. Among its enabling technologies, precision fermentation and cultivated-cell systems offer complementary advantages but continue to face challenges related to production costs, scalability, and functional performance. Increasingly, hybrid cellular agriculture approaches combining precision-fermented proteins, cultivated cells, and plant-derived matrices are being explored to overcome these limitations and accelerate commercialization. This review examines recombinant proteins as critical enabling components within these integrated systems. Advances in microbial expression platforms, including prokaryotic hosts such as Escherichia coli and Bacillus subtilis and eukaryotic hosts such as Saccharomyces cerevisiae and Komagataella phaffii, are critically evaluated regarding protein yield, product quality, regulatory suitability, downstream processing, and techno-economic feasibility. Industrial-scale fermentation capacities up to 80,000 L demonstrate the growing potential for large-scale recombinant protein production. Applications of recombinant proteins in edible scaffolds, serum-free culture media, extracellular matrix alternatives, and functional food ingredients are discussed alongside their associated technical and regulatory challenges. Ultimately, recombinant proteins are identified as integrative elements bridging acellular and cell-based production systems, supporting the development of scalable, economically viable, and sustainable cellular agriculture.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.2,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green fabrication and antibacterial evaluation of lysozyme-immobilized cysteine-capped silver nanoparticles. 溶菌酶固定化半胱氨酸包覆纳米银的绿色制备及抗菌性能评价。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-04 DOI: 10.1080/10826068.2026.2682259

Hira Ateeq, Aabgeena Naeem, Talha Rauf, Hamna Jamaeel, Mohammad Owais

{"title":"Green fabrication and antibacterial evaluation of lysozyme-immobilized cysteine-capped silver nanoparticles.","authors":"Hira Ateeq, Aabgeena Naeem, Talha Rauf, Hamna Jamaeel, Mohammad Owais","doi":"10.1080/10826068.2026.2682259","DOIUrl":"10.1080/10826068.2026.2682259","url":null,"abstract":"Aim: The broad-spectrum antibacterial properties of silver nanoparticles (AgNPs) are attracting increasing attention as a means to combat drug-resistant infections. Herein, we describe the green production of AgNPs with minimal environmental impact by using plant components as reducing agents.Methods and results: Hibiscus rosa sinensis flower extract was used to form AgNPs from a 1 mM solution of silver nitrate. The NPs were cysteine-stabilized, on which hen egg white lysozyme was immobilized. The NPs, as analyzed by SEM, were nearly spherical. The size of the Cys-NPs, as measured by TEM, ranged from 12.52 nm to 27.20 nm, with an average size of 22.83, and the size of Lys-Cys-AgNPs ranged from 16.32 nm to 44.60 nm, with an average size of 30.30 nm. The enzyme assay for Lys-Cys-AgNPs demonstrated an effective activity of approximately 80.8 ± 3.6% compared to free lysozyme, and the immobilization efficiency was calculated to be 66.8%. MIC of Lys-Cys-AgNPs against E. coli (8.5 μg/mL), P. aeruginosa (12 μg/mL), S.aureus (6 μg/mL) and S. mutans (5.5 μg/mL). The antibacterial activity of Lys-Cys-AgNPs combined with streptomycin was evaluated against E. coli, P. aeruginosa, S. aureus, and S. mutans using the agar well diffusion method. The zones of inhibition observed were 11.00 ± 0.31 mm, 8.00 ± 0.11 mm, 8.00 ± 0.14 mm and 18.00 ± 0.65 mm, respectively.Conclusion: Green-synthesized, cysteine-stabilized silver nanoparticles functionalized with lysozyme demonstrated enhanced antibacterial efficacy against both Gram-positive and Gram-negative pathogens. These findings highlight their potential as an eco-friendly and effective nanobiotherapeutic strategy to combat drug-resistant infections.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148387757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green production of zinc oxide nanorods for efficient removal of textile dyes. 高效去除纺织染料的氧化锌纳米棒的绿色生产。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-04 DOI: 10.1080/10826068.2026.2696343

Joshua Toyin Adeleke, Thirugnanasambandan Theivasanthi, Kalaivanan Poomari, Yetunde A Ajayeoba, Ayomide Lois Adeleke, Ayoola Yemisi, Ephriam Akor, Subash C B Gopinath, Moses O Alfred

{"title":"Green production of zinc oxide nanorods for efficient removal of textile dyes.","authors":"Joshua Toyin Adeleke, Thirugnanasambandan Theivasanthi, Kalaivanan Poomari, Yetunde A Ajayeoba, Ayomide Lois Adeleke, Ayoola Yemisi, Ephriam Akor, Subash C B Gopinath, Moses O Alfred","doi":"10.1080/10826068.2026.2696343","DOIUrl":"10.1080/10826068.2026.2696343","url":null,"abstract":"This study shows the effect of Zinc oxide nanorods, synthesized using Moringa oleifera phytochemicals as reducing and stabilizing agents were synthesized via a green method and systematically characterized to establish a consistent dataset linking structure, properties, and photocatalytic activity. Energy-dispersive X-ray spectroscopy confirmed the presence of only Zn and O elements, while scanning electron microscopy showed uniformly dispersed rod-like morphologies with particle sizes in the range of 10-25 nm. Powder X-ray diffraction confirmed a crystalline hexagonal wurtzite phase with an average crystallite size of 7.56 nm. The photocatalytic efficiency of ZnO nanorods was estimated using methylene blue, Congo red, and rhodamine B dyes as model pollutants, in addition to real textile wastewater samples. The treated wastes showed improved dissolved oxygen from 0.4 to 2.13 mg/L and lowered BOD from 930 to 30 mg/L. These data show the reproducibility and efficiency of green synthesis of ZnO nanoparticles using M. oleifera for wastewater purification.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-15"},"PeriodicalIF":2.2,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148387854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mannich-modified benzaldehyde chitosan as a capping agent for silver nanocomposites: enhanced antibacterial performance for food preservation. 曼尼基改性苯甲醛壳聚糖作为银纳米复合材料的封盖剂：提高食品保鲜的抗菌性能。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-04 DOI: 10.1080/10826068.2026.2673209

Ismiyarto Ismiyarto, Nurul Alya Husin, Ngadiwiyana Ngadiwiyana, Purbowatiningrum Ria Sarjono, Hasan Muhtar

{"title":"Mannich-modified benzaldehyde chitosan as a capping agent for silver nanocomposites: enhanced antibacterial performance for food preservation.","authors":"Ismiyarto Ismiyarto, Nurul Alya Husin, Ngadiwiyana Ngadiwiyana, Purbowatiningrum Ria Sarjono, Hasan Muhtar","doi":"10.1080/10826068.2026.2673209","DOIUrl":"https://doi.org/10.1080/10826068.2026.2673209","url":null,"abstract":"Chitosan, a biocompatible and biodegradable biopolymer, was chemically modified via a one-pot Mannich reaction under reflux to synthesize benzaldehyde-based β-aminocarbonyl chitosan (BACC-B). The obtained BACC-B was a brown solid with a yield of 75.5% (w/w) and a degree of substitution (DS) of 23%. To enhance antibacterial performance, BACC-B was incorporated with silver (Ag) to form BACC-B/Ag composites, where BACC-B acted as a capping agent to inhibit Ag agglomeration. Composites were synthesized using 0, 1, 3, 6, 10, and 15 mol equivalents of ascorbic acid relative to 1 mol equivalent of AgNO3 precursor, while chitosan/Ag (6 equivalents) was prepared as a comparison. Characterization was conducted using UV-Vis spectrophotometry, FTIR, and AAS for Ag quantification. The 6-equivalent BACC-B/Ag composite exhibited the most balanced performance, with a yield of 45.80% and a surface plasmon resonance (SPR) peak at 433 nm, indicating nanoparticle formation. Chitosan/Ag showed an SPR peak at 424 nm with a higher Ag content (0.73%) compared to BACC-B/Ag (0.57%). All composites formed greenish-brown solids. Antibacterial activity evaluated by the total plate count (TPC) method on chicken meat during 0, 3, and 7 days of storage demonstrated significant bacterial reduction. The BACC-B (2500 ppm) and BACC-B/Ag (2500 ppm) samples showed reductions of 92.30% and 96.33%, respectively, on day 3, indicating strong short-term antibacterial potential for food preservation applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel cold-active alkaline protease from Bacillus mobilis TK31: random mutagenesis, characterization, and its potential for industrial applications. 一种来自移动芽孢杆菌TK31的新型冷活性碱性蛋白酶：随机诱变、特性及其工业应用潜力。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-04 DOI: 10.1080/10826068.2026.2682261

Tuna Karaytuğ, Burhan Arıkan

{"title":"A novel cold-active alkaline protease from Bacillus mobilis TK31: random mutagenesis, characterization, and its potential for industrial applications.","authors":"Tuna Karaytuğ, Burhan Arıkan","doi":"10.1080/10826068.2026.2682261","DOIUrl":"10.1080/10826068.2026.2682261","url":null,"abstract":"This study introduces a novel cold-active alkaline protease-producing Bacillus mobilis TK31 strain, first isolated from Türkiye and identified through biochemical and molecular analyses. Combined mutagenesis using UV irradiation and ethyl methanesulfonate (EMS) enhanced protease production of the strain under optimized conditions. Proteases from wild-type and mutant strains were partially purified by acetone precipitation and subjected to preliminary comparative characterization. Both enzymes exhibited optimal activity at a pH of 9.0 and a temperature of 15 °C. The mutant 18C-179 enzyme showed a 3.75-fold activity increase over the wild-type post-optimization. The enzymes' pH and temperature stability, chemical resistance, and substrate specificity were evaluated, with the mutant displaying superior stability at extreme pH values, higher temperatures, and enhanced resistance to specific chemicals. Casein hydrolysis products were identified chromatographically. The wild-type and mutant enzymes demonstrated promising potential in detergent, photography, and pharmaceutical applications. This work presents a cold-active alkaline protease and highlights its potential for further industrial biotechnology studies through strain improvement.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-19"},"PeriodicalIF":2.2,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural deep eutectic solvent for efficient extraction of polyphenols from dendrobium residue: optimization, characterization, and enhanced antioxidant activity. 从石斛渣中高效提取多酚的天然深共熔溶剂：优化、表征和增强抗氧化活性。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-04 DOI: 10.1080/10826068.2026.2673208

Xian Hu, Minghui Fang, HePing Huang, Naidong Chen, Jingwen Hao

{"title":"Natural deep eutectic solvent for efficient extraction of polyphenols from dendrobium residue: optimization, characterization, and enhanced antioxidant activity.","authors":"Xian Hu, Minghui Fang, HePing Huang, Naidong Chen, Jingwen Hao","doi":"10.1080/10826068.2026.2673208","DOIUrl":"https://doi.org/10.1080/10826068.2026.2673208","url":null,"abstract":"This study optimized the extraction of polyphenols from Dendrobium residue using a natural deep eutectic solvent (NADES). A Box-Behnken design determined the optimal conditions: a choline chloride to glucose molar ratio of 2.4:1, solid-to-solvent ratio of 1:61 g/mL, water content of 35.7%, temperature of 80 °C, and duration of 120 minutes. This yielded a polyphenol content of 1.381 ± 0.071%, which was 2.93 to 38.36 times higher than conventional organic solvent methods. Fourier transform infrared (FTIR) spectroscopy was used to characterize the hydrogen bonding changes in the NADES system, while scanning electron microscopy (SEM) was employed to examine the morphological differences of Dendrobium residues before and after extraction treatment. Ten compounds were identified from the NADES extract, namely Prunin, Schaftoside, Apigenin-6,8-di-C-α-l-arabinopyranoside, Batatasin III, Moscatilin, Dihydroresveratrol, Hesperetin, Naringenin, Dendrophenol, and Erianin, which exhibited important pharmacological activities. Ultra-performance liquid chromatography(UPLC) analysis revealed a great variety and quantity of polyphenols in the NADES extract. Furthermore, the extract exhibited superior antioxidant activity: at 1.00 mg/mL, DPPH scavenging was 90.615 ± 0.92%, ABTS scavenging was 96.095 ± 0.44%, and FRAP value was 1.048 ± 0.03 mmol/L. These results highlight the green and efficient nature of this NADES-based protocol for industrial applications in food and herb residue processing.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-17"},"PeriodicalIF":2.2,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgae cultivation in treated landfill leachate: biomass productivity and lipid characterization aiming biodiesel production. 微藻在处理过的垃圾渗滤液中的培养：生物质生产力和针对生物柴油生产的脂质表征。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-04 DOI: 10.1080/10826068.2026.2682265

Patrícia Caroline Molgero Da Rós, Phydias Bianchi Pereira, Isabella Canela Ceu, Vitor Bertolassi Hidalgo, Adriano Francisco Siqueira, Leandro Gonçalves de Aguiar, Marco Aurélio Kondracki de Alcântara

{"title":"Microalgae cultivation in treated landfill leachate: biomass productivity and lipid characterization aiming biodiesel production.","authors":"Patrícia Caroline Molgero Da Rós, Phydias Bianchi Pereira, Isabella Canela Ceu, Vitor Bertolassi Hidalgo, Adriano Francisco Siqueira, Leandro Gonçalves de Aguiar, Marco Aurélio Kondracki de Alcântara","doi":"10.1080/10826068.2026.2682265","DOIUrl":"10.1080/10826068.2026.2682265","url":null,"abstract":"Producing biodiesel from alternative biomass sources is a promising strategy for sustainable energy systems. This study evaluated urban landfill leachate treated by an advanced oxidative process (AOP) as an alternative to synthetic media for microalgae cultivation aimed at biodiesel production. The leachate was treated under ultraviolet (UV) irradiation with hydrogen peroxide in a photochemical system. A 22 factorial design assessed the effects of UV lamp power (0, 15, and 28 W) and hydrogen peroxide feeding time (90, 120, and 150 min). UV power significantly affected leachate degradation, reaching 57.3% reduction in total organic carbon. Treated leachate was then used as a culture medium for Nannochloropsis gaditana at concentrations of 50%, 80%, and 100% (v/v). The highest biomass productivity (381.62 mg L-1 day-1) and lipid productivity (115.87 mg L-1 day-1) were obtained with 50% treated leachate combined with conventional medium. Fatty acid analysis showed predominance of saturated and monounsaturated fatty acids, indicating favorable cetane number and oxidative stability, despite possible cold-flow limitations. The results demonstrate the potential of treated landfill leachate for microalgal cultivation and biodiesel production within a circular bioeconomy approach.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0