Preparative Biochemistry & Biotechnology最新文献_第3页

Screening and stability verification of reference genes in Botrytis cinerea ZX2 fermentation. 肉毒芽孢杆菌 ZX2 发酵中参考基因的筛选和稳定性验证。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-11 DOI: 10.1080/10826068.2024.2405944

Yifan Wang, Dan Shu, Zhemin Li, Di Luo, Jie Yang, Tianfu Li, Xiaonan Hou, Qi Yang, Hong Tan

{"title":"Screening and stability verification of reference genes in Botrytis cinerea ZX2 fermentation.","authors":"Yifan Wang, Dan Shu, Zhemin Li, Di Luo, Jie Yang, Tianfu Li, Xiaonan Hou, Qi Yang, Hong Tan","doi":"10.1080/10826068.2024.2405944","DOIUrl":"10.1080/10826068.2024.2405944","url":null,"abstract":"Botrytis cinerea, an airborne plant pathogen, holds the potential to synthesize sesquiterpenes, which have been used for the industrial production of abscisic acid. Previously, through our genetic technology, we obtained strain ZX2, whose main product 1´,4´-trans-ABA-diol is physiologically active in plants. In this study, 50 L of fed fermentation was carried out with ZX2 strain to study the stability of expression of TUA, TUB, ATC, EF-1, GAPDH, UCE and GTP genes. Four kinds of software (GeNorm, NormFinder, BestKeeper and Delta Ct) were used to analyze the expression stability of candidate genes, and finally the best reference gene was screened by RefFinder. Based on the results, the ACT was the most stable gene. It was used to normalize the expression levels of two genes related to 1´,4´-trans-ABA-diol production (hmgr and bcaba3) when fed-batch fermentation. Guide the selection of appropriate internal reference genes during the fermentation process to accurately quantify the relative transcription levels of target genes in B.cinerea ZX2.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-8"},"PeriodicalIF":2.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethanol and 2-phenylethanol production by bee-isolated Meyerozyma caribbica strains. 蜜蜂分离的 Meyerozyma caribbica 菌株产生乙醇和 2-苯基乙醇。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-10 DOI: 10.1080/10826068.2024.2414094

Eduardo Dias Fenner, Stéfany Kell Bressan, Angela Alves Dos Santos, Anderson Giehl, Gabriel do Amaral Minussi, Elisa Amorim Amâncio Teixeira, Mariana da Costa Diniz, Larissa Werlang, Odinei Fogolari, Carlos Augusto Rosa, Helen Treichel, Liziara da Costa Cabrera, Sergio Luiz Alves Junior

{"title":"Ethanol and 2-phenylethanol production by bee-isolated Meyerozyma caribbica strains.","authors":"Eduardo Dias Fenner, Stéfany Kell Bressan, Angela Alves Dos Santos, Anderson Giehl, Gabriel do Amaral Minussi, Elisa Amorim Amâncio Teixeira, Mariana da Costa Diniz, Larissa Werlang, Odinei Fogolari, Carlos Augusto Rosa, Helen Treichel, Liziara da Costa Cabrera, Sergio Luiz Alves Junior","doi":"10.1080/10826068.2024.2414094","DOIUrl":"https://doi.org/10.1080/10826068.2024.2414094","url":null,"abstract":"Investigating the biotechnological potential of wild microorganisms is paramount for optimizing bioprocesses. Given this premise, we looked for yeasts in Brazilian native stingless bees, considering the recognized potential of pollinating insect-associated microorganisms for the production of volatile organic compounds (VOCs). Two yeast strains of the species Meyerozyma caribbica were isolated from bees Scaptotrigona postica and evaluated for their fermentative capacity. Both yeasts were capable of fermenting sucrose (the main sugar used in the Brazilian ethanol industry) with over 90% efficiency and yields of up to 0.504 g/g. Through an experimental design analysis (CCD), it was verified that the ethanol productivity of these yeasts can also benefit from high concentrations of sucrose and low pH values, desirable traits for microorganisms in this biofuel production. At the same time, CCD analyses also showed the great capacity of these M. caribbica strains to produce another alcohol of broad biotechnological interest, 2-phenylethanol. Interestingly, the statistical analyses demonstrated that greater production of this compound can occur at high sugar concentrations and low availability of nitrogen sources, which can be easily achieved using residual low-cost feedstocks. Thus, our results suggest that these M. caribbica strains may be efficiently used in both ethanol and 2-phenylethanol production.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maximizing polyphenol yield: ultrasound-assisted extraction and antimicrobial potential of mango peel. 最大限度地提高多酚产量：芒果皮的超声波辅助提取和抗菌潜力。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-02 DOI: 10.1080/10826068.2024.2411518

Md Shohag Sarker, Md Moktadirul Alam, Chen Jiao, Wu Shuqi, Li Xiaohui, Nasir Ali, L O Mallasiy, Azizah A Alshehri

{"title":"Maximizing polyphenol yield: ultrasound-assisted extraction and antimicrobial potential of mango peel.","authors":"Md Shohag Sarker, Md Moktadirul Alam, Chen Jiao, Wu Shuqi, Li Xiaohui, Nasir Ali, L O Mallasiy, Azizah A Alshehri","doi":"10.1080/10826068.2024.2411518","DOIUrl":"https://doi.org/10.1080/10826068.2024.2411518","url":null,"abstract":"This study investigated the ultrasound-assisted extraction (UAE) techniques used to enhance the polyphenols and antioxidants of mango peel extract (MPE). Additionally, it explored the bacteriostatic activity of MPE against various microorganisms. The UAE method was optimized using response surface methodology (RSM) at different times, temperatures, and ratios, with optimal conditions found to be 35 minutes, 45 °C, and a 1:35 ratio. The optimized yield results for total polyphenol content (TPC) were 17.33 ± 1.57 mg GAE/g, total flavonoid content (TFC) was 12.14 ± 0.29 mg QE/g, and radical scavenging activity (RSA) was 72.11 ± 2.19%. These response models were extremely significant with p-values less than 0.05. MPE showed selective effectiveness against Bacillus cereus, Geobacillus stearothermophilus, and Escherichia coli (E. coli). The results highlight the potential of mango peel as a sustainable source of bioactive compounds, contributing to waste reduction in the food industry and the development of natural antimicrobial agents. This study contributes to further research on the application of MPE in processed foods.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of membrane separation technology in the purification of pharmaceutical components. 膜分离技术在药物成分纯化中的应用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-25 DOI: 10.1080/10826068.2024.2328673

Yun Bi, Jingyi Dong, Yujia Zhou, Manyue Zhang, Xingying Chen, Yuyan Zhang

引用次数: 0

Microwave-enhanced hydrolysis of cellulose by acidic ionic liquids. 微波增强酸性离子液体对纤维素的水解作用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-23 DOI: 10.1080/10826068.2024.2333467

Meng-Meng Liu, Liang-Yan Zhang, Zhen Liu

引用次数: 0

Statistical versus neural network-embedded swarm intelligence optimization of a metallo-neutral-protease production: activity kinetics and food industry applications. 金属中性蛋白酶生产的统计与神经网络嵌入式群集智能优化：活性动力学与食品工业应用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-16 DOI: 10.1080/10826068.2024.2328681

Maurice George Ekpenyong, Sylvester Peter Antai

{"title":"Statistical versus neural network-embedded swarm intelligence optimization of a metallo-neutral-protease production: activity kinetics and food industry applications.","authors":"Maurice George Ekpenyong, Sylvester Peter Antai","doi":"10.1080/10826068.2024.2328681","DOIUrl":"10.1080/10826068.2024.2328681","url":null,"abstract":"An integrated approach involving response surface methodology (RSM) and artificial neural network-ant-colony hybrid optimization (ANN-ACO) was adopted to develop a bioprocess medium to increase the yield of Bacillus cereus neutral protease under submerged fermentation conditions. The ANN-ACO model was comparatively superior (predicted r2 = 98.5%, mean squared error [MSE] = 0.0353) to RSM model (predicted r2 = 86.4%, MSE = 23.85) in predictive capability arising from its low performance error. The hybrid model recommended a medium containing (gL-1) molasses 45.00, urea 9.81, casein 25.45, Ca2+ 1.23, Zn2+ 0.021, Mn2+ 0.020, and 4.45% (vv-1) inoculum, for a 6.75-fold increase in protease activity from a baseline of 76.63 UmL-1. Yield was further increased in a 5-L bioreactor to a final volumetric productivity of 3.472 mg(Lh)-1. The 10.0-fold purified 46.6-kDa-enzyme had maximum activity at pH 6.5, 45-55 °C, with Km of 6.92 mM, Vmax of 769.23 µmolmL-1 min-1, kcat of 28.49 s-1, and kcat/Km of 4.117 × 103 M-1 s-1, at 45 °C, pH 6.5. The enzyme was stabilized by Ca2+, activated by Zn2+ but inhibited by EDTA suggesting that it was a metallo-protease. The biomolecule significantly clarified orange and pineapple juices indicating its food industry application.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1132-1146"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140140627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of sugarcane bagasse for high-yield production of laccase through Aspergillus terreus for effective azo dye decolourization. 利用甘蔗渣的价值，通过土曲霉高产生产漆酶，有效去除偶氮染料。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-04-01 DOI: 10.1080/10826068.2024.2332881

T Chandukishore, Tuhin Subhra Biswas, Ashish A Prabhu

{"title":"Valorization of sugarcane bagasse for high-yield production of laccase through Aspergillus terreus for effective azo dye decolourization.","authors":"T Chandukishore, Tuhin Subhra Biswas, Ashish A Prabhu","doi":"10.1080/10826068.2024.2332881","DOIUrl":"10.1080/10826068.2024.2332881","url":null,"abstract":"Synthetic dyes such as azo dyes are significant pollutants in the wastewater released from various textile industries. The low biodegradability and production from synthetic sources with high shelf life make azo dyes a challenging material for degradation. This study used chemically mutated Aspergillus terrus in the laccase production under solid-state fermentation using sugarcane bagasse. Initially, the wild-type strain produced a laccase activity of 4.12 U/mL. Later, the alkaline pretreatment of sugarcane bagasse showed a significant increase in laccase activity by 38.9%. Further, random mutagenesis treatment with 100 mM EMS generated a hyper laccase-producing strain with a 2.3-fold increment in laccase activity compared to the wild-type strain. The enzyme displayed optimal activity at pH 6.5 and 35 °C. The metal ions such as Fe3+ (29.4 U/mL), Fe2+ (20.8 U/mL) and Cu2+ (18.05 U/mL) showed positive effects on laccase activity. The crude laccase was used to bioremediate Congo red, a prominent azo dye used in textile and pharmaceutical industries. The preliminary studies with a crude enzyme displayed 68.86% dye decolourization after 24 h of incubation. Additionally, with Taguchi orthogonal array optimization experiments, the maximal dye decolorization of 78.24% was achieved by maintaining crude enzyme concentration (20 U), dye concentration (25 mg/L) and pH 4.5.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1170-1181"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140336649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the efficiency of the Sec and Tat secretory pathways in the secretion of recombinant neurturin protein using de novo designed signal peptides. 利用全新设计的信号肽比较 Sec 和 Tat 分泌途径在重组新乌头素蛋白分泌中的效率。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-21 DOI: 10.1080/10826068.2024.2331203

Zahra Hajihassan, Mina Yazdi, Atiyeh Fadaie, Nooshin Akbarsemnani

{"title":"Comparison of the efficiency of the Sec and Tat secretory pathways in the secretion of recombinant neurturin protein using de novo designed signal peptides.","authors":"Zahra Hajihassan, Mina Yazdi, Atiyeh Fadaie, Nooshin Akbarsemnani","doi":"10.1080/10826068.2024.2331203","DOIUrl":"10.1080/10826068.2024.2331203","url":null,"abstract":"Since cytoplasmic expression of heterologous proteins with disulfide bonds leads to the formation of inclusion bodies in E. coli, periplasmic production is preferable. The N-terminal signal peptide attached to the secreted protein determines the type of secretory pathway through which the target protein is secreted; Sec, Tat, or SRP. The aim of this study was to design and compare two novel signal peptides for the secretion of recombinant neurturin (as a model) via the Sec and Tat pathways. For this purpose, we aligned the natural signal peptides from E. coli and Bacillus subtilis to identify the conserved amino acids and those with the highest repetition. The SignalP4.1 and TatP1.0 software were used to determine the secretion efficiency of the new signal peptides. The efficiency of new signal peptides was then evaluated and compared experimentally with two naturally used signal peptides. Quantitative analysis of Western blot bands showed that approximately 80% of the expressed neurturin was secreted into the periplasmic space by new signal peptides. Circular dichroism spectroscopy also confirmed the correct secondary structure of the secreted neurturin. In conclusion, these novel signal peptides can be used to secrete any other recombinant proteins to the periplasmic space of E. coli efficiently.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1157-1169"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140176123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of ultrasound to increase the catalytic activity of α-L-rhamnosidase. 利用超声波提高α-L-鼠李糖酶的催化活性。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-13 DOI: 10.1080/10826068.2024.2326877

Chen Qi, Le Li, Kunpeng Yu, Yanling Lin, Lijun Li

引用次数: 0

Employing soil isolated fungi for production of bioactive phenolic compounds: a fermentative approach. 利用土壤分离真菌生产生物活性酚类化合物：一种发酵方法。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-13 DOI: 10.1080/10826068.2024.2326882

Rathnaprabha Dharavath, Srividya A

{"title":"Employing soil isolated fungi for production of bioactive phenolic compounds: a fermentative approach.","authors":"Rathnaprabha Dharavath, Srividya A","doi":"10.1080/10826068.2024.2326882","DOIUrl":"10.1080/10826068.2024.2326882","url":null,"abstract":"An efficient method of solid-state fermentation (SSF) is reported for producing bioactive phenolic compounds using soil-isolated fungi. Antioxidant activity using a rapid DPPH (1,1-diphenyl-2-picryl hydrazyl), was employed to screen the 120 fungal isolates from soil. Aspergillus terreus 1, Aspergillus fumigatus, Aspergillus terreus 2, Penicillium citrinum, Aspergillus wentii1, Aspergillus wentii 2, Penicillium expansum and Penicillium granulatum were chosen, concerning their antioxidant activity and total phenolic content. These fungal strains were applied on agro residues viz. sugarcane bagasse, corn cob, rice straw, pea pod and wheat straw, to evaluate the release of phenolic compounds. The fermented extracts from various agro-residues showed good antioxidant activity against DPPH, ferric ion, and nitric oxide radicals. The highest antioxidant activity was observed in fermented extracts of sugarcane bagasse, followed by pea pod. Additionally, the total phenolic content in the fermented extracts positively correlated with antioxidant potential. This study highlights the significant potential of solid substrate fermentation using soil-isolated fungi and agro-residues to produce bioactive phenolic compounds with potent antioxidant properties. The utilization of SSF for the extraction of bioactive compounds from natural sources not only offers a clean and sustainable approach but also contributes to the valorization of agro-industrial residues.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1121-1131"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0