Preparative Biochemistry & Biotechnology最新文献_第3页

Uncovering the in vitro antibiofilm potential of Dittrichia graveolens silver nanoparticle against infectious agents.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-03-01 DOI: 10.1080/10826068.2025.2472937

Ayşe Karacalı Tunç, Ramazan Erenler

{"title":"Uncovering the in vitro antibiofilm potential of Dittrichia graveolens silver nanoparticle against infectious agents.","authors":"Ayşe Karacalı Tunç, Ramazan Erenler","doi":"10.1080/10826068.2025.2472937","DOIUrl":"https://doi.org/10.1080/10826068.2025.2472937","url":null,"abstract":"Dittrichia graveolens (L.) Desf., an aromatic plant, is used in various treatments in popular medicine. Especially its methanolic and aqueous extracts have many biological activities. Nanotechnology, which has pioneered important research in recent years, has also begun to be preferred in the field of health. Based on this, we aim to investigate the effect of silver nanoparticle synthesized from Dittrichia graveolens on antimicrobial and antibiofilm. We tried to destroy the biofilm structures responsible for antibiotic resistance in bacteria with environmentally friendly nanoparticles. The antimicrobial activity of AgNPs obtained through green synthesis, was evaluated by the liquid medium microdilution method. MIC (minimum inhibitory concentration) values of AgNPs for Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae strains were determined by the microdilution method in 96-well ELISA plates. The effect of AgNPs on biofilm was performed using the crystal violet method in 96-well flat-bottom microplates. The MIC values of the four standard strains were determined to be 128 μg/mL. All standard strains showed antibiofilm effects for every concentration of AgNPs. The lowest concentration of AgNPs for inhibition the biofilm was detected as about 90% at 16 μg/mL. It was concluded that green synthesized AgNPs was effective on bacteria. In addition, this is the first study on this subject. This study may be an innovative approach to the scientific world from a herbal and bacterial perspective.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-7"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of a genetically encoded biosensor for evaluating enterovirus 71 3C protease inhibitors.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-28 DOI: 10.1080/10826068.2025.2472952

Jiankai Ye, Rui Zhang, Xiaoping Liu, Yunyu Chen

{"title":"Production of a genetically encoded biosensor for evaluating enterovirus 71 3C protease inhibitors.","authors":"Jiankai Ye, Rui Zhang, Xiaoping Liu, Yunyu Chen","doi":"10.1080/10826068.2025.2472952","DOIUrl":"https://doi.org/10.1080/10826068.2025.2472952","url":null,"abstract":"Enterovirus 71 (EV-71) is a major causative agent of hand, foot, and mouth disease (HFMD), which mainly affects infants and children. However, there are no effective clinical drugs for the treatment of HFMD. The 3C protease (3Cpro) is an ideal drug target for antivirals, as this enzyme plays an indispensable role in virus replication. Considering the limitations of the peptide substrates used in the fluorescence resonance energy transfer (FRET) assay, there is an urgent need to design improved 3Cpro biosensors for assay development. In this study, we developed a genetically encoded biosensor based on a dimerization-dependent red fluorescent protein (ddRFP) system for evaluating 3Cpro inhibitors. The 3Cpro biosensor has many beneficial properties, such as economical bioproduction, a simple dual-mode readout, and a high emission wavelength. Using the 3Cpro biosensor, rupintrivir was identified as a competitive 3Cpro inhibitor in vitro. Our research highlights a promising avenue for producing 3Cpro biosensors from E. coli cells. The 3Cpro biosensor provides a reliable biochemical tool for the rapid assessment of antivirals against enterovirus infections.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-6"},"PeriodicalIF":2.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced phytoremediation and biosorption of cationic methylene blue dye by Acidomyces acidophilus ATCC 26774.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-27 DOI: 10.1080/10826068.2025.2471891

Jinwon Kim, Young-Kyoung Park, Myeong-Seon Ryu, Gwangsu Ha, Hee-Jong Yang, Do-Youn Jeong

{"title":"Enhanced phytoremediation and biosorption of cationic methylene blue dye by Acidomyces acidophilus ATCC 26774.","authors":"Jinwon Kim, Young-Kyoung Park, Myeong-Seon Ryu, Gwangsu Ha, Hee-Jong Yang, Do-Youn Jeong","doi":"10.1080/10826068.2025.2471891","DOIUrl":"https://doi.org/10.1080/10826068.2025.2471891","url":null,"abstract":"In this study, the enhancement of the phytoremediation and biosorption properties of the cationic dye methylene blue using Acidomyces acidophilus ATCC 26774 were investigated. Inoculation with A. acidophilus ATCC 26774 increased the length of Bassica juncea roots by 82.3% and stems by 37.7% in the presence of 50 mg/L MB dye. Furthermore, the dried biomass of A. acidophilus ATCC 26774 showed superior removal capacity (87.1%) of MB dye. The maximum adsorption capacities of the biomass were investigated at pH 11 (176.8 mg/g) and 0.09 g/20 ml dried biomass (120.3 mg/g), respectively. The influence of contact times (0-360 min) and initial concentrations (30.0-357.5 mg/L) on the biosorption of MB dye was also investigated. In addition, A. acidophilus ATCC 26774 was characterized by Fourier transform infrared spectroscopy (FT-IR) and point of zero charge (pHpzc). Equilibrium biosorption isotherms and kinetics results showed a Langmuir isotherm and pseudo-second-order kinetic models that fit well for MB dye biosorption. Monolayer biosorption, intraparticle diffusion, and chemisorption are predicted to play key roles in MB dye biosorption. In conclusion, A. acidophilus ATCC 26774 is proposed as an excellent plant growth promoting fungus and biosorbent with potential applications in the removal of cationic MB dye from the environment.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the effect of different organic solvents in the efficiency of the recovery of pullulan synthesized by Aureobasidium pullulans.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-27 DOI: 10.1080/10826068.2025.2471890

Gustavo Henrique Lisboa, Aline de Cassia Campos Pena, Andre Rodrigues, Sabrina Ávila Rodrigues

{"title":"Evaluation of the effect of different organic solvents in the efficiency of the recovery of pullulan synthesized by Aureobasidium pullulans.","authors":"Gustavo Henrique Lisboa, Aline de Cassia Campos Pena, Andre Rodrigues, Sabrina Ávila Rodrigues","doi":"10.1080/10826068.2025.2471890","DOIUrl":"https://doi.org/10.1080/10826068.2025.2471890","url":null,"abstract":"The production and extraction of microbial biopolymers face significant challenges, especially during the purification process. This study evaluated the production of pullulan under controlled conditions and its extraction using different organic solvents. Kinetic parameters such as cell growth rate, substrate consumption, biomass production and biopolymer yield were analyzed. Fermentation was conducted in a bioreactor, followed by solvent extraction of different chemical groups, including ketones, benzene, carboxylic esters, alkanes, and cycloalkanes. The production of the biopolymer started with 10 g/L after 15 hours of fermentation and reached 12 g/L at the end of the process. However, extraction using solvents such as Cyclohexane, Hexane, and Toluene was not effective due to inappropriate molecular interactions. On the other hand, Isopropyl Alcohol recovered 18.87 g/L of pullulan while Ethanol reached 12.82 g/L. Despite the higher yield with Isopropyl Alcohol, Ethanol stood out as the best alternative due to its low toxicity, reduced cost, and ease of handling. The results showed that Ethanol is optimal for the extraction process. Still, it represents a practical approach to the recovery of this biopolymer.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-pot green and sustainable process for the biotransformation of sophoricoside to genistein from Fructus Sophorae with magnetic cellulose spheres immobilized Aspergillus oryzae on cellulose using deep eutectic solvent assisted.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-27 DOI: 10.1080/10826068.2025.2471892

Shuang Jin, Yubin Ren, Hongyao Cai, Biqiong Chen, Yupeng Cheng, Weili Liu, Cailiang Peng, Yujie Fu, Chen Lv, Huiling Li

{"title":"One-pot green and sustainable process for the biotransformation of sophoricoside to genistein from Fructus Sophorae with magnetic cellulose spheres immobilized Aspergillus oryzae on cellulose using deep eutectic solvent assisted.","authors":"Shuang Jin, Yubin Ren, Hongyao Cai, Biqiong Chen, Yupeng Cheng, Weili Liu, Cailiang Peng, Yujie Fu, Chen Lv, Huiling Li","doi":"10.1080/10826068.2025.2471892","DOIUrl":"https://doi.org/10.1080/10826068.2025.2471892","url":null,"abstract":"This study introduces a green, sustainable, and efficient approach for biotransforming sophoricoside into genistein from Fructus Sophorae using Aspergillus oryzae by removal of one molecule of glucose by β-glucosidase, an edible microorganism immobilized on magnetic cellulose and treated with deep eutectic solvents (DES). The goal was to enhance the biotransformation ratio by optimizing reaction conditions and selecting the most suitable DES. Various DESs, including natural deep eutectic solvents (NADES), were assessed for their ability to improve catalytic performance. Among them, the NADES system comprising choline chloride (CHCL) and glycerol (G) exhibited the highest catalytic efficiency (32.19 mg/g) under optimal conditions: temperature 33 °C, time 65 hours, pH 5.5, and a liquid-to-solid ratio of 45:1 (mL/g). This yield was 10.60 times greater than the genistein yield from untreated F. Sophorae. This combination notably increased cell membrane permeability, aiding the bioconversion process. The cellulose immobilization technique provided a stable and reusable microreactor and maintained microbial activity (80.37%) over 10 cycles. These findings validate the bioconversion method as a promising and sustainable strategy for genistein production from plant-derived sophoricoside, with potential applications in pharmaceutical and nutraceutical industries.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy chain variants affect light and heavy chains assembly of monoclonal antibody expressed by Pichia pastoris.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-24 DOI: 10.1080/10826068.2025.2470209

Xiulong Gong, Kaixiong Ye, Mingqiang Xu, Zhilan Qian, Qi Liu, Xiaolong Wang, Xiangshan Zhou, Haifeng Liu, Menghao Cai

{"title":"Heavy chain variants affect light and heavy chains assembly of monoclonal antibody expressed by Pichia pastoris.","authors":"Xiulong Gong, Kaixiong Ye, Mingqiang Xu, Zhilan Qian, Qi Liu, Xiaolong Wang, Xiangshan Zhou, Haifeng Liu, Menghao Cai","doi":"10.1080/10826068.2025.2470209","DOIUrl":"https://doi.org/10.1080/10826068.2025.2470209","url":null,"abstract":"Monoclonal Antibody accounts for the largest share of recombinant protein drugs and is the primary choice for the treatment of various diseases. In this study, the monoclonal antibody Eptinezumab was expressed by a yeast host Pichia pastoris. Although the expression and secretion of light chain was efficient, the assembly efficiency between light and heavy chains was low. As the retention of the heavy chain in endoplasmic reticulum may trigger protein degradation, ERAD ubiquitination-related genes were then knocked out separately but it only led to minor improvement effect. Expression of splitted heavy chain variants further revealed that although endoplasmic reticulum retention of the heavy chain upregulated KAR2 expression, it did not affect the assembly efficiency of the light and heavy chains. It was inferred that binding of complete heavy chains to KAR2 spatially affected the assembly between light and heavy chains. Design and screening of KAR2 variants that facilitating full-length antibody assembly could be preferentially considered in future.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-8"},"PeriodicalIF":2.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a scalable semi-synthetic medium for high-yield diphtheria toxin production using a definitive screening design an innovative statistical optimization method.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-21 DOI: 10.1080/10826068.2025.2465991

Prashant R Chawla, Uma Addepally

{"title":"Development of a scalable semi-synthetic medium for high-yield diphtheria toxin production using a definitive screening design an innovative statistical optimization method.","authors":"Prashant R Chawla, Uma Addepally","doi":"10.1080/10826068.2025.2465991","DOIUrl":"https://doi.org/10.1080/10826068.2025.2465991","url":null,"abstract":"Corynebacterium diphtheriae is the causative agent of diphtheria, which continues to be a serious health risk to children, particularly in countries such as India. Immunization is the best way to fight this illness. Enhancing the synthesis of diphtheria toxin (DT) is essential for the production of vaccines, particularly as immunization programs advance. Pork digestion medium (PDM) was employed as the standard medium for DT production. Nevertheless, this medium has issues with contamination and batch-to-batch variation. The production of DT is extremely low in the alternative synthetic medium. Compared to synthetic media, semi-synthetic media exhibit superior performance. This study's current goal was to increase DT production through the use of the Definitive Screening Design (DSD) methodology to optimize the composition of semi-synthetic media. A total of 11 components were selected for screening of the best suitable components for DT production. NZ-amine, tryptone N1, and maltose had the highest effects on DT production out of all the nutrients that were chosen. The model accuracy is indicated by the R2 value of 0.9820, which enables the prediction of DT yields. The model suggests the lower concentrations of NZ-amine combined with the moderate amounts of maltose and tryptone N1 is best suitable for the higher amounts of DT yields. With optimized conditions 174 Lf/mL of DT yield was achieved in validation experiments, which is nearer to the PDM yields. According to this study, this enhanced technique, which makes use of an affordable and expandable medium, could make large-scale toxoid production feasible.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-8"},"PeriodicalIF":2.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of proteases for animal feed supplementation: scientific and technological updates.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-20 DOI: 10.1080/10826068.2025.2465957

Tiago de Oliveira Sousa, Neurimar Araújo da Silva, Vagne de Melo Oliveira, Alan Vagner da Silva Ramos, José Pedro Martins Barbosa Filho, Juanize Matias da Silva Batista, Romero Marcos Pedrosa Brandão Costa, Ana Lúcia Figueiredo Porto, Stélio Bezerra Pinheiro de Lima, Maurício de Paula Ferreira Teixeira, Thiago Pajeú Nascimento

{"title":"Use of proteases for animal feed supplementation: scientific and technological updates.","authors":"Tiago de Oliveira Sousa, Neurimar Araújo da Silva, Vagne de Melo Oliveira, Alan Vagner da Silva Ramos, José Pedro Martins Barbosa Filho, Juanize Matias da Silva Batista, Romero Marcos Pedrosa Brandão Costa, Ana Lúcia Figueiredo Porto, Stélio Bezerra Pinheiro de Lima, Maurício de Paula Ferreira Teixeira, Thiago Pajeú Nascimento","doi":"10.1080/10826068.2025.2465957","DOIUrl":"https://doi.org/10.1080/10826068.2025.2465957","url":null,"abstract":"The global market is rapidly evolving, with biotechnological advances supporting production centers through innovative techniques. A key focus is on bioprocesses for extracting, purifying, and applying proteases, particularly in animal feed within the agricultural sector. Although the cost reduction may vary depending on the animal species, supplementation with proteases allows for the formulation of more cost-effective diets, improving nutrient digestibility and absorption, optimizing energy efficiency, preserving intestinal integrity, enhancing carcass quality, modulating the intestinal microbiome, and reducing nitrogen excretion. This study aimed to update on protease use in monogastric animal feed, drawing from digital databases (Science Direct, Google Scholar, and Scielo) and industrial property databases (INPI). Despite the significant potential of proteases in monogastric animal nutrition, there is a lack of studies, especially on patented technologies. While their benefits are recognized, substantial investments are needed to develop new technologies and identify alternative protease sources. These advancements could enhance animal performance and strengthen Brazil's productive sector, highlighting the need for further research and innovation. The literature review showed promising results, but research is mainly focused on microbial proteases for poultry, pigs, and fish. There is significant potential to explore diverse protease production sources and their application across various species.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From probiotic fermentation to functional drinks: a review on fruit juices with lactic acid bacteria and prebiotics.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-19 DOI: 10.1080/10826068.2025.2467441

Nurhazwani Sa'aid, Joo Shun Tan

{"title":"From probiotic fermentation to functional drinks: a review on fruit juices with lactic acid bacteria and prebiotics.","authors":"Nurhazwani Sa'aid, Joo Shun Tan","doi":"10.1080/10826068.2025.2467441","DOIUrl":"https://doi.org/10.1080/10826068.2025.2467441","url":null,"abstract":"In recent years, the demand for probiotic beverages has surged, with dairy products traditionally serving as the primary sources of probiotics. However, many consumers face health issues such as lactose intolerance, milk allergies, and high cholesterol, which prevent them from consuming dairy products. This has led to the exploration of nondairy alternatives, particularly fruit juices, as carriers for probiotics. Lactic acid bacteria (LAB) have been identified as beneficial probiotics that can be incorporated into these beverages. The inclusion of prebiotics, such as inulin and galacto-oligosaccharides (GOS), in fruit juices has shown promise in enhancing the growth and activity of LAB, thereby creating functional beverages that support digestive health. Despite numerous studies on fruit juice fermentation, there is limited data on the optimal pairing of probiotics and prebiotics to develop stable, nondairy functional drinks. This review underscores the potential of lactic acid fermentation and the integration of prebiotics and probiotics in fruit juices, highlighting the necessity for further research to optimize these combinations for enhanced health benefits and improved beverage stability.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-20"},"PeriodicalIF":2.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized extraction of Spirulina platensis phenolics using natural deep eutectic solvents for cosmetics.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-19 DOI: 10.1080/10826068.2025.2467440

Semra Nur Kamil, Selin Tezcanlı, Yaren Çelik, İrem Toprakçı, Selin Şahin

{"title":"Optimized extraction of Spirulina platensis phenolics using natural deep eutectic solvents for cosmetics.","authors":"Semra Nur Kamil, Selin Tezcanlı, Yaren Çelik, İrem Toprakçı, Selin Şahin","doi":"10.1080/10826068.2025.2467440","DOIUrl":"https://doi.org/10.1080/10826068.2025.2467440","url":null,"abstract":"This study presents a highly environmentally friendly system using natural deep eutectic solvent (NADES) as an organic solvent substitute to obtain high-quality microalgae extract, which is a potential new source of moisturizing cosmetic materials. Blue-green algae Spirulina platensis was selected as the alg metabolite source. Choline chloride and glycerol were used as the ingredients of the NADES owing to they are commonly used in cosmetic products for skin moisturizing and/or healing. The extraction of bioactives from Spirulina platensis was optimized based on the multi-response optimizition of Response Surface method (RSM). 3-factor and 3-level Box-Behnken design was implemented for the design matrix with 17 experimental runs. The best conditions were attained by almost 21% (v/v) water addition into the best NADES (choline chloride/glycerol, ½ molar ratio) for 30 sec of extraction time under almost 7500 rpm mixing speed. The given conditions provided the highest yields of total phenolic content (10.517 mg-GAE/g-DM), antioxidant activity (1.796 mg-TEAC/g-DM) and 1,2,3,4-butanetetrol (70.174%), which were approved with satisfactory validation findings (the error < 2%). The application of the produced products gave promising findings for the valorization of the NADES-based alg metabolites as potential natural additives used directly in cosmetic formulations.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0