Preparative Biochemistry & Biotechnology最新文献_第4页

Application of membrane separation technology in the purification of pharmaceutical components. 膜分离技术在药物成分纯化中的应用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-25 DOI: 10.1080/10826068.2024.2328673

Yun Bi, Jingyi Dong, Yujia Zhou, Manyue Zhang, Xingying Chen, Yuyan Zhang

引用次数: 0

Microwave-enhanced hydrolysis of cellulose by acidic ionic liquids. 微波增强酸性离子液体对纤维素的水解作用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-23 DOI: 10.1080/10826068.2024.2333467

Meng-Meng Liu, Liang-Yan Zhang, Zhen Liu

引用次数: 0

Statistical versus neural network-embedded swarm intelligence optimization of a metallo-neutral-protease production: activity kinetics and food industry applications. 金属中性蛋白酶生产的统计与神经网络嵌入式群集智能优化：活性动力学与食品工业应用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-16 DOI: 10.1080/10826068.2024.2328681

Maurice George Ekpenyong, Sylvester Peter Antai

{"title":"Statistical versus neural network-embedded swarm intelligence optimization of a metallo-neutral-protease production: activity kinetics and food industry applications.","authors":"Maurice George Ekpenyong, Sylvester Peter Antai","doi":"10.1080/10826068.2024.2328681","DOIUrl":"10.1080/10826068.2024.2328681","url":null,"abstract":"An integrated approach involving response surface methodology (RSM) and artificial neural network-ant-colony hybrid optimization (ANN-ACO) was adopted to develop a bioprocess medium to increase the yield of Bacillus cereus neutral protease under submerged fermentation conditions. The ANN-ACO model was comparatively superior (predicted r2 = 98.5%, mean squared error [MSE] = 0.0353) to RSM model (predicted r2 = 86.4%, MSE = 23.85) in predictive capability arising from its low performance error. The hybrid model recommended a medium containing (gL-1) molasses 45.00, urea 9.81, casein 25.45, Ca2+ 1.23, Zn2+ 0.021, Mn2+ 0.020, and 4.45% (vv-1) inoculum, for a 6.75-fold increase in protease activity from a baseline of 76.63 UmL-1. Yield was further increased in a 5-L bioreactor to a final volumetric productivity of 3.472 mg(Lh)-1. The 10.0-fold purified 46.6-kDa-enzyme had maximum activity at pH 6.5, 45-55 °C, with Km of 6.92 mM, Vmax of 769.23 µmolmL-1 min-1, kcat of 28.49 s-1, and kcat/Km of 4.117 × 103 M-1 s-1, at 45 °C, pH 6.5. The enzyme was stabilized by Ca2+, activated by Zn2+ but inhibited by EDTA suggesting that it was a metallo-protease. The biomolecule significantly clarified orange and pineapple juices indicating its food industry application.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1132-1146"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140140627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of sugarcane bagasse for high-yield production of laccase through Aspergillus terreus for effective azo dye decolourization. 利用甘蔗渣的价值，通过土曲霉高产生产漆酶，有效去除偶氮染料。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-04-01 DOI: 10.1080/10826068.2024.2332881

T Chandukishore, Tuhin Subhra Biswas, Ashish A Prabhu

{"title":"Valorization of sugarcane bagasse for high-yield production of laccase through Aspergillus terreus for effective azo dye decolourization.","authors":"T Chandukishore, Tuhin Subhra Biswas, Ashish A Prabhu","doi":"10.1080/10826068.2024.2332881","DOIUrl":"10.1080/10826068.2024.2332881","url":null,"abstract":"Synthetic dyes such as azo dyes are significant pollutants in the wastewater released from various textile industries. The low biodegradability and production from synthetic sources with high shelf life make azo dyes a challenging material for degradation. This study used chemically mutated Aspergillus terrus in the laccase production under solid-state fermentation using sugarcane bagasse. Initially, the wild-type strain produced a laccase activity of 4.12 U/mL. Later, the alkaline pretreatment of sugarcane bagasse showed a significant increase in laccase activity by 38.9%. Further, random mutagenesis treatment with 100 mM EMS generated a hyper laccase-producing strain with a 2.3-fold increment in laccase activity compared to the wild-type strain. The enzyme displayed optimal activity at pH 6.5 and 35 °C. The metal ions such as Fe3+ (29.4 U/mL), Fe2+ (20.8 U/mL) and Cu2+ (18.05 U/mL) showed positive effects on laccase activity. The crude laccase was used to bioremediate Congo red, a prominent azo dye used in textile and pharmaceutical industries. The preliminary studies with a crude enzyme displayed 68.86% dye decolourization after 24 h of incubation. Additionally, with Taguchi orthogonal array optimization experiments, the maximal dye decolorization of 78.24% was achieved by maintaining crude enzyme concentration (20 U), dye concentration (25 mg/L) and pH 4.5.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1170-1181"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140336649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the efficiency of the Sec and Tat secretory pathways in the secretion of recombinant neurturin protein using de novo designed signal peptides. 利用全新设计的信号肽比较 Sec 和 Tat 分泌途径在重组新乌头素蛋白分泌中的效率。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-21 DOI: 10.1080/10826068.2024.2331203

Zahra Hajihassan, Mina Yazdi, Atiyeh Fadaie, Nooshin Akbarsemnani

{"title":"Comparison of the efficiency of the Sec and Tat secretory pathways in the secretion of recombinant neurturin protein using de novo designed signal peptides.","authors":"Zahra Hajihassan, Mina Yazdi, Atiyeh Fadaie, Nooshin Akbarsemnani","doi":"10.1080/10826068.2024.2331203","DOIUrl":"10.1080/10826068.2024.2331203","url":null,"abstract":"Since cytoplasmic expression of heterologous proteins with disulfide bonds leads to the formation of inclusion bodies in E. coli, periplasmic production is preferable. The N-terminal signal peptide attached to the secreted protein determines the type of secretory pathway through which the target protein is secreted; Sec, Tat, or SRP. The aim of this study was to design and compare two novel signal peptides for the secretion of recombinant neurturin (as a model) via the Sec and Tat pathways. For this purpose, we aligned the natural signal peptides from E. coli and Bacillus subtilis to identify the conserved amino acids and those with the highest repetition. The SignalP4.1 and TatP1.0 software were used to determine the secretion efficiency of the new signal peptides. The efficiency of new signal peptides was then evaluated and compared experimentally with two naturally used signal peptides. Quantitative analysis of Western blot bands showed that approximately 80% of the expressed neurturin was secreted into the periplasmic space by new signal peptides. Circular dichroism spectroscopy also confirmed the correct secondary structure of the secreted neurturin. In conclusion, these novel signal peptides can be used to secrete any other recombinant proteins to the periplasmic space of E. coli efficiently.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1157-1169"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140176123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of ultrasound to increase the catalytic activity of α-L-rhamnosidase. 利用超声波提高α-L-鼠李糖酶的催化活性。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-13 DOI: 10.1080/10826068.2024.2326877

Chen Qi, Le Li, Kunpeng Yu, Yanling Lin, Lijun Li

引用次数: 0

Employing soil isolated fungi for production of bioactive phenolic compounds: a fermentative approach. 利用土壤分离真菌生产生物活性酚类化合物：一种发酵方法。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-13 DOI: 10.1080/10826068.2024.2326882

Rathnaprabha Dharavath, Srividya A

{"title":"Employing soil isolated fungi for production of bioactive phenolic compounds: a fermentative approach.","authors":"Rathnaprabha Dharavath, Srividya A","doi":"10.1080/10826068.2024.2326882","DOIUrl":"10.1080/10826068.2024.2326882","url":null,"abstract":"An efficient method of solid-state fermentation (SSF) is reported for producing bioactive phenolic compounds using soil-isolated fungi. Antioxidant activity using a rapid DPPH (1,1-diphenyl-2-picryl hydrazyl), was employed to screen the 120 fungal isolates from soil. Aspergillus terreus 1, Aspergillus fumigatus, Aspergillus terreus 2, Penicillium citrinum, Aspergillus wentii1, Aspergillus wentii 2, Penicillium expansum and Penicillium granulatum were chosen, concerning their antioxidant activity and total phenolic content. These fungal strains were applied on agro residues viz. sugarcane bagasse, corn cob, rice straw, pea pod and wheat straw, to evaluate the release of phenolic compounds. The fermented extracts from various agro-residues showed good antioxidant activity against DPPH, ferric ion, and nitric oxide radicals. The highest antioxidant activity was observed in fermented extracts of sugarcane bagasse, followed by pea pod. Additionally, the total phenolic content in the fermented extracts positively correlated with antioxidant potential. This study highlights the significant potential of solid substrate fermentation using soil-isolated fungi and agro-residues to produce bioactive phenolic compounds with potent antioxidant properties. The utilization of SSF for the extraction of bioactive compounds from natural sources not only offers a clean and sustainable approach but also contributes to the valorization of agro-industrial residues.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1121-1131"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insulin-transferrin-selenium promote formation of tissue-engineered vascular grafts in early stage of culture. 胰岛素-转铁蛋白-硒可促进组织工程血管移植物早期培养的形成。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-28 DOI: 10.1080/10826068.2024.2333468

Xuheng Sun, Nannan Wang, Hongjing Jiang, Qing Liu, Cong Xiao, Jianyi Xu, Yindi Wu, Jingyi Mei, Shuting Wu, Zhanyi Lin

{"title":"Insulin-transferrin-selenium promote formation of tissue-engineered vascular grafts in early stage of culture.","authors":"Xuheng Sun, Nannan Wang, Hongjing Jiang, Qing Liu, Cong Xiao, Jianyi Xu, Yindi Wu, Jingyi Mei, Shuting Wu, Zhanyi Lin","doi":"10.1080/10826068.2024.2333468","DOIUrl":"10.1080/10826068.2024.2333468","url":null,"abstract":"To create tissue-engineered vascular grafts (TEVGs) in vitro, vascular smooth muscle cells (VSMCs) must function effectively and produce sufficient extracellular matrix (ECM) in a three-dimensional space. In this study, we investigated whether the addition of insulin-transferrin-selenium (ITS), a medium supplement, could enhance TEVG formation. PGA fabric was used as the scaffold, and 1% ITS was added to the medium. After two weeks, the tissues were examined using electron microscopy and staining. The ITS group exhibited a denser structure and increased collagen production. VSMCs were cultured in two dimensions with ITS and assessed for collagen production, cell growth, and glucose metabolism. The results showed that ITS supplementation increased collagen production, cell growth, glucose utilization, lactate production, and ATP levels. Furthermore, reducing the amount of fetal bovine serum (FBS) in the medium did not affect the TEVGs or VSMCs when ITS was present. In conclusion, ITS improves TEVG construction by promoting VSMCs growth and reducing the need for FBS.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1186-1195"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental validation off-line a nonlinear controller for removal of chromium using non-living cells of Yarrowia lipolytica. 利用脂肪溶解亚罗菌的非活细胞去除铬的离线非线性控制器的实验验证。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-27 DOI: 10.1080/10826068.2024.2329277

Vicente Peña Caballero, Pablo A López-Pérez, García Salas Oatna Georgina, Adan T Morales-Vargas

{"title":"Experimental validation off-line a nonlinear controller for removal of chromium using non-living cells of Yarrowia lipolytica.","authors":"Vicente Peña Caballero, Pablo A López-Pérez, García Salas Oatna Georgina, Adan T Morales-Vargas","doi":"10.1080/10826068.2024.2329277","DOIUrl":"10.1080/10826068.2024.2329277","url":null,"abstract":"The removal of hexavalent chromium [Cr (VI)] using non-living cells of Yarrowia lipolytica was investigated. Batch and continuous studies on removal of Cr (VI) achieved 97% and 99% removal from aqueous phase, respectively. The specific uptake values at pH of 2 in batch process were 40.73 ± 1.3 mg/g and 30.09 ± 0.23 mg/g on non-living cells, when 100 and 200 mg/L of metal Cr (VI) concentrations were used. In order to investigate the regulation of Cr (VI) under continuous operation based on reaction volume numerically a new class of feedback controller from structure polynomial was designed. The proposed methodology was used to an experimentally kinetic model for a removal Cr (VI) from Yarrowia lipolytica biomass was showed satisfactory closed-loop performance the proposed controller. Starting from an off-line optimization performed in simulation, we present the controller implementation, focussing on the methodology required to could be suitable for implementation in real time. In our experimental results, we highlight some discrepancies between simulation and reality despite these differences, the controller managed to perform convergence to removal Cr (VI). Finally, the results validated with off-line samples suggest that the proposed control could be suitable for in application in potential scenarios for wastewater treatment.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1147-1156"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140294330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and identification of a salt-tolerant Coelastrum sp. and exploration of its potential for biodiesel production. 分离和鉴定一种耐盐鹅掌霉菌并探索其生产生物柴油的潜力。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-24 DOI: 10.1080/10826068.2024.2405941

Jing Xu, Han Wang, Jixin Liu, Jingping Ge, Yimeng Lin, Wenxiang Ping

引用次数: 0