Preparative Biochemistry & Biotechnology最新文献_第4页

Impacts of conventional and industrial wastewaters-based media on biomass production, nutrient dynamics, and fatty acid profile in a thraustochytrid culture. 传统培养基和工业废水培养基对蓟马培养过程中生物量生产、营养动态和脂肪酸组成的影响。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-04 DOI: 10.1080/10826068.2025.2484606

Pedro Contreras, Claudia Oviedo, Robinson Soto-Ramírez, Cinthia Vásquez-Sandoval, José Navarrete, Patricio Dantagnan

{"title":"Impacts of conventional and industrial wastewaters-based media on biomass production, nutrient dynamics, and fatty acid profile in a thraustochytrid culture.","authors":"Pedro Contreras, Claudia Oviedo, Robinson Soto-Ramírez, Cinthia Vásquez-Sandoval, José Navarrete, Patricio Dantagnan","doi":"10.1080/10826068.2025.2484606","DOIUrl":"https://doi.org/10.1080/10826068.2025.2484606","url":null,"abstract":"The rising demand for polyunsaturated fatty acids, coupled with the decline of traditional fish-based sources, highlights Ulkenia visurgensis Lng2, a newly isolated thraustochytrid strain, as a promising alternative for sustainable polyunsaturated fatty acids production. Using Response Surface Methodology to optimize medium composition and culture conditions, biomass production was improved to 11.64 g/L-120% higher than previous studies-with 35% of the biomass consisting of total lipids. Of the total fatty acids, 40% corresponded to polyunsaturated fatty acids, including 27% of docosahexaenoic acid, 4% of eicosapentaenoic acid, and 7% of arachidonic acid. Alternative media, such as wastewaters from corn and fish meal processing, were also evaluated. Biomass yields in wastewaters-based media (30% v/v) were lower due to limited nutrient availability, achieving between 2.96 g/L and 2.28 g/L, with the strain showing mostly around 8% carbon and nitrogen assimilation. Morphological changes, including increased vegetative and multinucleated cells, were also observed. Lipid content decreased in wastewater-based cultures, yet PUFAs constituted 47% of total fatty acids, with EPA nearly doubling, DHA remaining stable and decreased ARA content. These results shed light on how U. visurgensis Lng2 adapts to nutrient-limited environments and offer valuable insights for developing sustainable PUFA production using industrial by-products as alternative media.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of different light spectra on selenium bioaccumulation by Spirulina platensis cyanobacteria in flat plate photobioreactors. 平板光生物反应器中不同光谱对蓝藻硒生物累积的影响

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-11-11 DOI: 10.1080/10826068.2024.2426744

Yeganeh Shirazi, Salar Helchi, Mir Mehrshad Emamshoushtari, Sina Niakan, Elnaz Sohani, Farshid Pajoum Shariati

{"title":"The effect of different light spectra on selenium bioaccumulation by Spirulina platensis cyanobacteria in flat plate photobioreactors.","authors":"Yeganeh Shirazi, Salar Helchi, Mir Mehrshad Emamshoushtari, Sina Niakan, Elnaz Sohani, Farshid Pajoum Shariati","doi":"10.1080/10826068.2024.2426744","DOIUrl":"10.1080/10826068.2024.2426744","url":null,"abstract":"Selenium (Se) plays a crucial role in human health, influencing conditions such as cancer, diabetes, and neurological disorders. With global population growth and unequal nutrient distribution threatening food security, new approaches are needed to meet the nutritional needs of the world. Se is essential for immune function, metabolism, and antioxidant defense, and in regions suffering from food insecurity and malnutrition, selenium-enriched food could offer an affordable solution. Spirulina platensis, microalgae, can bioaccumulate Se from its environment, enhancing its nutritional value. This study explores how different light spectra (red, white, yellow, and blue LEDs) affect Se bioaccumulation in Spirulina when Na2SeO3 is added to the culture medium in photobioreactors. The results show that red light made the highest Se bioaccumulation (0.118 mg.L-1), followed by white, yellow, and blue light. Se addition also increased cell dry weight by 46%, 33%, 22%, and 60%, respectively, compared to photobioreactors without Se, with biomass productivity highest under red light. Furthermore, Se boosted maximum Chl α concentration, improving photosynthetic efficiency. These findings suggest that optimizing light conditions can significantly enhance the nutritional value of Spirulina, offering a potential solution to global hunger by providing a sustainable, selenium-enriched food source.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"491-501"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered production of 5-aminolevulinic acid in recombinant Escherichia coli BL21. 在重组大肠杆菌 BL21 中设计生产 5-氨基乙酰丙酸。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-11-05 DOI: 10.1080/10826068.2024.2423644

Zhiwen Zhu, Bing Fu, Jiajie Lu, Peize Wang, Chuyang Yan, Fuyao Guan, Jianying Huang, Ping Yu

{"title":"Engineered production of 5-aminolevulinic acid in recombinant Escherichia coli BL21.","authors":"Zhiwen Zhu, Bing Fu, Jiajie Lu, Peize Wang, Chuyang Yan, Fuyao Guan, Jianying Huang, Ping Yu","doi":"10.1080/10826068.2024.2423644","DOIUrl":"10.1080/10826068.2024.2423644","url":null,"abstract":"5-aminolevulinic acid (ALA) is a non-protein amino acid that has been widely used in the fields of medicine and agriculture. This study aims to engineer the C5 pathway of the ALA biosynthesis in Escherichia coli BL21 to enhance ALA production. The ALA synthase genes gltX, hemA, and hemL were overexpressed in E. coli BL21 to lead to the increase in the production of ALA. The sRNA RyhB was also overexpressed to downregulate the expression of ALA dehydratase to reduce the downstream bioconversion of ALA to porphobilinogen. Next, the gene arcA was knocked out by CRISPR-Cas9 technology to open the TCA cycle to promote the respiratory metabolism of the strain to reduce the feedback inhibition of heme to ALA. The fermentation conditions of the engineered strain were optimized by response surface experiments. The time-course analysis of the ALA production was carried out in a 1 L shake flask. Through these efforts, the production of ALA in engineered strain reached 2953 mg/L in a 1 L shake flask. This study contributes to the industrial production of ALA by the engineered E. coli in the future.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"446-456"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variability in growth and biosynthetic activity of Calendula officinalis hairy roots. 金盏花毛根的生长和生物合成活性的变化。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-10-21 DOI: 10.1080/10826068.2024.2418015

Nadiia Matvieieva, Taisa Bohdanovych, Valeriia Belokurova, Volodymyr Duplij, Anatolii Shakhovsky, Dmytro Klymchuk, Mykola Kuchuk

{"title":"Variability in growth and biosynthetic activity of Calendula officinalis hairy roots.","authors":"Nadiia Matvieieva, Taisa Bohdanovych, Valeriia Belokurova, Volodymyr Duplij, Anatolii Shakhovsky, Dmytro Klymchuk, Mykola Kuchuk","doi":"10.1080/10826068.2024.2418015","DOIUrl":"10.1080/10826068.2024.2418015","url":null,"abstract":"Calendula officinalis is a widespread medicinal plant with a sufficiently well-studied chemical composition. Secondary metabolites synthesized by C.officinalis plants have pharmacological value for treating numerous diseases, and various types of aseptic in vitro cultures can be used as a source of these compounds. From this perspective, hairy roots attract considerable attention for the production of bioactive chemicals, including flavonoids with antioxidant activity. This paper shows the possibility of C.officinalis hairy roots obtaining with 100% frequency by Agrobacterium rhizogenes genetic transformation. Hairy root lines differed in growth rate and flavonoid content. In particular, flavonoids were accumulated in the amount of up to 6.68 ± 0.28 mg/g of wet weight. Methyl jasmonate in the concentration of 10 µM inhibited root growth to a small extent but stimulated the synthesis of flavonoids. The antioxidant activity and the reducing power increased in the roots grown in the medium with methyl jasmonate. The strong correlation of antioxidant activity and reducing power with flavonoid content was detected. The influence of extraction conditions on the content of flavonoids in the extracts and their bioactivity was determined. The potent reducing activity of extracts from hairy roots allowed the production of silver nanoparticles, which was confirmed by transmission electron microscopy.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"381-391"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A UHPLC-QE-MS-based metabolomics approach for the evaluation of fermented lipase by an engineered Escherichia coli. 基于uhplc - qe - ms的代谢组学方法评价工程大肠杆菌发酵脂肪酶。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-12-08 DOI: 10.1080/10826068.2024.2423665

Jun Zhang, Ying Zhang, Wen Luo, Zhiyuan Wang, Pengmei Lv, Zhongming Wang

{"title":"A UHPLC-QE-MS-based metabolomics approach for the evaluation of fermented lipase by an engineered Escherichia coli.","authors":"Jun Zhang, Ying Zhang, Wen Luo, Zhiyuan Wang, Pengmei Lv, Zhongming Wang","doi":"10.1080/10826068.2024.2423665","DOIUrl":"10.1080/10826068.2024.2423665","url":null,"abstract":"Using an engineered Escherichia coli to produce lipase and can easily achieve high-level expression. The investigation of biochemical processes during lipase fermentation, approached from a metabolomics perspective, will yield novel insights into the efficient secretion of recombinant proteins. In this study, the lipase batch fermentation was carried out first with enzyme activity of 36.83 U/mg cells. Then, differential metabolites and metabolic pathways were identified using an untargeted metabolomics approach through comparative analysis of various fermentation periods. In total, 574 metabolites were identified: 545 were up-regulated and 29 were down-regulated, mainly in 153 organic acids and derivatives, 160 organoheterocyclic compounds, 64 lipids and lipid-like molecules, and 58 organic oxygen compounds. Through metabolic pathways and network analysis, it could be found that tryptophan metabolism was of great significance to lipase production, which could affect the secretion and synthesis of recombinant protein. In addition, the promotion effects of cell growth by varying concentrations of indole acetic acid serve to validate the results obtained from tryptophan metabolism. This study offers valuable insights into metabolic regulation of engineered E. coli, indicating that its fermentation bioprocess can be systematically designed according to metabolomics findings to enhance recombinant protein production.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"457-469"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-assisted extraction of Stachys chasmosericea: phytochemical profile, antioxidant potentials via RACI analysis, and enzyme inhibitory activities. 超声波辅助提取毛丝桃的植物化学特征、抗氧化活性及酶抑制活性。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 DOI: 10.1080/10826068.2025.2486414

Fatma Ozlem Kargin Solmaz, Nilay Isitez, Cengiz Sarikurkcu

{"title":"Ultrasound-assisted extraction of Stachys chasmosericea: phytochemical profile, antioxidant potentials via RACI analysis, and enzyme inhibitory activities.","authors":"Fatma Ozlem Kargin Solmaz, Nilay Isitez, Cengiz Sarikurkcu","doi":"10.1080/10826068.2025.2486414","DOIUrl":"https://doi.org/10.1080/10826068.2025.2486414","url":null,"abstract":"Understanding the bioactive potential of plant extracts requires detailed evaluations of their chemical composition and bioactivities. This study investigated the chemical profiles, antioxidant properties, and enzyme inhibitory activities of water and methanol extracts of Stachys chasmosericea Ayasligil & P.H.Davis obtained via ultrasound-assisted extraction. The methanol extract displayed significantly higher total phenolic and flavonoid contents compared to the water extract. Chromatographic analysis revealed verbascoside and chlorogenic acid as the dominant compounds in the methanol extract, whereas the water extract contained higher levels of polar compounds like p-coumaric acid. Antioxidant assays demonstrated the methanol extract's superior activity. Conversely, the water extract excelled in metal chelation. RACI values confirmed the methanol extract's overall antioxidant superiority (RACI = 0.65) over the water extract (RACI = -0.65). Enzyme inhibition assays revealed the methanol extract's stronger activity against AChE, BChE, α-amylase, α-glucosidase, and tyrosinase whereas the water extract showed weaker inhibition. Both extracts demonstrated potential for managing oxidative stress and enzyme-related disorders but require further in vivo validation. This study highlights S. chasmosericea, particularly its methanol extract, as a promising source of natural antioxidants and enzyme inhibitors. Future research should focus on isolating individual bioactives and exploring their mechanisms of action in biological systems.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of ultrasound-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharide from Plantaginis Semen. 超声辅助深共晶溶剂萃取的优化、表征以及植物精液多糖的生物活性。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-11-04 DOI: 10.1080/10826068.2024.2423640

Yao Wen, Geping Chen, Lei Hu

{"title":"Optimization of ultrasound-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharide from Plantaginis Semen.","authors":"Yao Wen, Geping Chen, Lei Hu","doi":"10.1080/10826068.2024.2423640","DOIUrl":"10.1080/10826068.2024.2423640","url":null,"abstract":"Plantaginis Semen (PS) are the dried mature seeds of Plantago asiatica L. or Plantagodepressa Willd. in the Plantago family. Its polysaccharides are important components of PS. Response surface methodology was used to optimize the ultrasonic-assisted deep eutectic solvent (DES) extraction process of PS polysaccharides (PSP). The results showed that the optimal extraction parameters were a solid-liquid ratio of 1:35 g/mL, an extraction time of 73 min, and a molar ratio of 2:1. The yield of PSP was 0.64% and 1.20% by water immersion and ultrasonic water extraction, respectively, indicating that the DES extraction method (2.21 ± 0.06%) is superior to these two methods, and the optimization effect is good. Through the α-glucosidase and α-amylase inhibition activities experiment, it was found that the IC50 values of PSPs-1 were 1122 and 220.5 μg/mL. DPPH·and ABTS+ scavenging activity experiments showed that the IC50 values of PSPs-1 were 19.2 and 4.3 μg/mL, respectively. Its molar ratio of monosaccharide composition is rhamnose: galactose: galacturonic acid: glucose: glucuronic acid: arabinose: mannose: xylose = 33.6:13.3:6.5:3:2.6:2:1.4:1. Therefore, this study can provide an experimental basis for the establishment of an industrialized production process of polysaccharides and the study of their biological activities.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"434-445"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of different extraction methods on the structure and antioxidant properties of Bletilla striata polysaccharide. 不同提取方法对条纹叶紫苏多糖结构和抗氧化性的影响

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-10-30 DOI: 10.1080/10826068.2024.2419862

Xue Han, Hai Liu, Yeshan Zhang, Yi Zhang, Zhiqin Song, Lili Yang, Xiao Liu, Lin Yang, Mingkai Wu, Longyan Tan

{"title":"The effects of different extraction methods on the structure and antioxidant properties of Bletilla striata polysaccharide.","authors":"Xue Han, Hai Liu, Yeshan Zhang, Yi Zhang, Zhiqin Song, Lili Yang, Xiao Liu, Lin Yang, Mingkai Wu, Longyan Tan","doi":"10.1080/10826068.2024.2419862","DOIUrl":"10.1080/10826068.2024.2419862","url":null,"abstract":"Bletilla striata polysaccharide (BSP) is one of the main active ingredients of the traditional Chinese medicine Bletilla striata (Thunb) Richb.f and the extraction method of BSP has a significant impact on its properties. This study investigated the effects of four extraction methods, namely hot water extraction, ultrasonic extraction, enzyme extraction, and microwave extraction, on the structure and antioxidant properties of BSP. Characterization results from FTIR and NMR showed that all four BSP consisted mainly of glucose and mannose, forming α-glycosidic and β-glycosidic linkages to form glucomannan. Hot water extraction had the lowest extraction rate of BSP at 21.78% ± 0.73%. The polysaccharide BSP-H obtained from hot water extraction had the smallest absolute Zeta potential and Grain size, but the largest molecular weight at 204 kDa. It exhibited the best thermal stability and superior antioxidant activity compared to polysaccharides extracted using the other three methods, as evaluated by three different antioxidant assays. Although the antioxidant activity of BSP-V was slightly weaker, it showed a significant improvement compared to the remaining two polysaccharides. These results suggest that hot water extraction is the most suitable method for large-scale application of BSP, preserving its activity effectively, thus facilitating practical production and product development.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"392-402"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of microwave-assisted extraction using response surface methodology and HPLC-DAD phenolic compounds quantification from Hylocereus undatus peel and pulp cultivated in Tunisia. 利用响应面方法优化微波辅助萃取，并对突尼斯种植的 Hylocereus undatus 果皮和果肉中的 HPLC-DAD 酚类化合物进行定量。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-11-06 DOI: 10.1080/10826068.2024.2423636

Wissal Ayouni, Hajer Riguene, Amira Yahyaoui, Marwa Dhiabi, Souad Dali, Houcine Ammar, Sirine Choura, Mohamed Chamkha, Ridha Ben Salem, Rigane Ghayth

{"title":"Optimization of microwave-assisted extraction using response surface methodology and HPLC-DAD phenolic compounds quantification from Hylocereus undatus peel and pulp cultivated in Tunisia.","authors":"Wissal Ayouni, Hajer Riguene, Amira Yahyaoui, Marwa Dhiabi, Souad Dali, Houcine Ammar, Sirine Choura, Mohamed Chamkha, Ridha Ben Salem, Rigane Ghayth","doi":"10.1080/10826068.2024.2423636","DOIUrl":"10.1080/10826068.2024.2423636","url":null,"abstract":"The present study aimed to optimize the microwave-assisted extraction process for both the peel and pulp of Hylocereus undatus (white dragon fruit) cultivated in Tunisia, using response surface methodology. Total phenolic content, total flavonoid content, FRAP (ferric reducing antioxidant power), and DPPH (1,1-diphenyl-2-picrylhydrazyl) antioxidant activities were optimized. A central composite design (CCD) was applied, considering three key variables: extraction time, extraction temperature, and liquid-to-solid ratio. The optimized extraction parameters for Hylocereus undatus peel and pulp were determined respectively (9.57 min, 42.20 °C and 27.79 mL/g) and (10.08 min, 40.84 °C and 31.52 mL/g). The main phenolic compounds identified in Hylocereus undatus peel and pulp extracts using HPLC-DAD were chlorogenic and caffeic acids and rutin, quercetin, luteolin-7-O-glucoside as flavonoids. Therefore, this research has revealed the potential of a sustainable and eco-friendly process hold promise a directional option and encouraging a circular economy approach for industrial production of antioxidant-rich Hylocereus undatus extracts.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"417-433"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulase immobilization on nano-chitosan/chromium metal-organic framework hybrid matrix for efficient conversion of lignocellulosic biomass to glucose. 将纤维素酶固定在纳米壳聚糖/铬金属有机框架混合基质上，实现木质纤维素生物质到葡萄糖的高效转化。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-11-14 DOI: 10.1080/10826068.2024.2425970

Shashi Suhag, Utkarsh Jain, Nidhi Chauhan, Vinita Hooda

{"title":"Cellulase immobilization on nano-chitosan/chromium metal-organic framework hybrid matrix for efficient conversion of lignocellulosic biomass to glucose.","authors":"Shashi Suhag, Utkarsh Jain, Nidhi Chauhan, Vinita Hooda","doi":"10.1080/10826068.2024.2425970","DOIUrl":"10.1080/10826068.2024.2425970","url":null,"abstract":"In the current work, cellulase from Aspergillus niger was successfully immobilized on a novel epoxy-affixed chromium metal-organic framework/chitosan (Cr@-MIL-101/CS) support via covalent method using glutaraldehyde as a crosslinker. The bare and cellulase-bound support was characterized by using various microscopic and spectroscopic techniques. Immobilized cellulase exhibited a high immobilization yield of 0.7 ± 0.01 mg/cm2, retaining 87.5 ± 0.04% of its specific activity and displaying enhanced catalytic performance. The immobilized enzyme was maximally active at pH 5.0, temperature 65 °C and 0.9 × 10-2 mg/ml saturating substrate concentration and the half-lives of free and immobilized cellulases were approximately 9 and 19 days, respectively. The decrease in activation energy, enthalpy change, and Gibbs free energy change, coupled with an increase in entropy change upon immobilization, indicated that the enzyme's efficiency, stability, and spontaneity in catalyzing the reaction were enhanced by immobilization. Additionally, the immobilized cellulase efficiently converted rice husk cellulose to glucose, with a quantification limit of 0.05%, linear measurement ranging from 0.1 to 0.9%, and 8.5% conversion efficiency. The present method exhibited a strong correlation (R2 = 0.998) with the DNS method, validating its reliability. Notably, the epoxy/Cr@-MIL-101/CS-bound cellulase demonstrated impressive thermal and pH stabilities, retaining 50% of its activity at 75 °C and over 96% at pH levels of 4.5 and 5.0 after 12 h. Furthermore, it showed excellent reusability, preserving 80% of its activity after 15 cycles and maintaining 50% of its activity even after 20 days of storage. These results suggest that epoxy/Cr@-MIL-101/CS/cellulase composites could be very effective for large-scale cellulose hydrolysis applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"470-490"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0