Impacts of conventional and industrial wastewaters-based media on biomass production, nutrient dynamics, and fatty acid profile in a thraustochytrid culture.

IF 2 4区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS
Pedro Contreras, Claudia Oviedo, Robinson Soto-Ramírez, Cinthia Vásquez-Sandoval, José Navarrete, Patricio Dantagnan
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Abstract

The rising demand for polyunsaturated fatty acids, coupled with the decline of traditional fish-based sources, highlights Ulkenia visurgensis Lng2, a newly isolated thraustochytrid strain, as a promising alternative for sustainable polyunsaturated fatty acids production. Using Response Surface Methodology to optimize medium composition and culture conditions, biomass production was improved to 11.64 g/L-120% higher than previous studies-with 35% of the biomass consisting of total lipids. Of the total fatty acids, 40% corresponded to polyunsaturated fatty acids, including 27% of docosahexaenoic acid, 4% of eicosapentaenoic acid, and 7% of arachidonic acid. Alternative media, such as wastewaters from corn and fish meal processing, were also evaluated. Biomass yields in wastewaters-based media (30% v/v) were lower due to limited nutrient availability, achieving between 2.96 g/L and 2.28 g/L, with the strain showing mostly around 8% carbon and nitrogen assimilation. Morphological changes, including increased vegetative and multinucleated cells, were also observed. Lipid content decreased in wastewater-based cultures, yet PUFAs constituted 47% of total fatty acids, with EPA nearly doubling, DHA remaining stable and decreased ARA content. These results shed light on how U. visurgensis Lng2 adapts to nutrient-limited environments and offer valuable insights for developing sustainable PUFA production using industrial by-products as alternative media.

传统培养基和工业废水培养基对蓟马培养过程中生物量生产、营养动态和脂肪酸组成的影响。
随着对多不饱和脂肪酸需求的不断增长,加上传统鱼类来源的减少,新分离的单胞菌Ulkenia visurgensis ln2成为可持续生产多不饱和脂肪酸的有希望的替代品。利用响应面法优化培养基组成和培养条件,生物量产量提高到11.64 g/ l,比以前的研究提高了120%,其中总脂质占生物量的35%。在总脂肪酸中,40%属于多不饱和脂肪酸,其中二十二碳六烯酸占27%,二十碳五烯酸占4%,花生四烯酸占7%。还对玉米和鱼粉加工废水等替代介质进行了评价。由于养分利用率有限,废水基培养基(30% v/v)的生物质产量较低,达到2.96 g/L至2.28 g/L之间,菌株的碳氮同化率大多在8%左右。形态学改变,包括增加的营养细胞和多核细胞,也被观察到。在废水基培养物中,脂质含量下降,但PUFAs占总脂肪酸的47%,EPA几乎翻了一番,DHA保持稳定,ARA含量下降。这些结果揭示了U. visurgensis Lng2如何适应营养有限的环境,并为利用工业副产品作为替代介质开发可持续的PUFA生产提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Preparative Biochemistry & Biotechnology
Preparative Biochemistry & Biotechnology 工程技术-生化研究方法
CiteScore
4.90
自引率
3.40%
发文量
98
审稿时长
2 months
期刊介绍: Preparative Biochemistry & Biotechnology is an international forum for rapid dissemination of high quality research results dealing with all aspects of preparative techniques in biochemistry, biotechnology and other life science disciplines.
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