Impacts of conventional and industrial wastewaters-based media on biomass production, nutrient dynamics, and fatty acid profile in a thraustochytrid culture.
Pedro Contreras, Claudia Oviedo, Robinson Soto-Ramírez, Cinthia Vásquez-Sandoval, José Navarrete, Patricio Dantagnan
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引用次数: 0
Abstract
The rising demand for polyunsaturated fatty acids, coupled with the decline of traditional fish-based sources, highlights Ulkenia visurgensis Lng2, a newly isolated thraustochytrid strain, as a promising alternative for sustainable polyunsaturated fatty acids production. Using Response Surface Methodology to optimize medium composition and culture conditions, biomass production was improved to 11.64 g/L-120% higher than previous studies-with 35% of the biomass consisting of total lipids. Of the total fatty acids, 40% corresponded to polyunsaturated fatty acids, including 27% of docosahexaenoic acid, 4% of eicosapentaenoic acid, and 7% of arachidonic acid. Alternative media, such as wastewaters from corn and fish meal processing, were also evaluated. Biomass yields in wastewaters-based media (30% v/v) were lower due to limited nutrient availability, achieving between 2.96 g/L and 2.28 g/L, with the strain showing mostly around 8% carbon and nitrogen assimilation. Morphological changes, including increased vegetative and multinucleated cells, were also observed. Lipid content decreased in wastewater-based cultures, yet PUFAs constituted 47% of total fatty acids, with EPA nearly doubling, DHA remaining stable and decreased ARA content. These results shed light on how U. visurgensis Lng2 adapts to nutrient-limited environments and offer valuable insights for developing sustainable PUFA production using industrial by-products as alternative media.
期刊介绍:
Preparative Biochemistry & Biotechnology is an international forum for rapid dissemination of high quality research results dealing with all aspects of preparative techniques in biochemistry, biotechnology and other life science disciplines.