Optimized production of ligninolytic enzymes by immobilized Phanerochaete chrysosporium in a lab-scale bioreactor.

Abstract

Effluents are a challenge for the textile industries, as they cannot be released into the environment without proper treatment. Biodegradation has been increasingly explored as an alternative for the treatment of this type of effluent, allowing the co-production of molecules of commercial interest. The purpose of this work is to evaluate the production of enzymes lignin peroxidase and manganese peroxidase from textile effluent by Phanerochaete chrysosporium. A response surface experimental design was developed to optimize the production of biomass, in which the independent variables were pH, carbon/nitrogen and phosphorus ratio, and the dependent variable was the production of biomass. The present work showed an activity of manganese peroxidase of 15.32 U L^-1 and lignin peroxidase of 70 U L^-1, demonstrating that the studied process is promising for the production of ligninolytic enzymes. This work demonstrates that the fungus P. chrysosporium can tolerate the adverse conditions of real textile effluent and use it as a source of nutrients for the production of biomass. Two enzymes were produced with applications in the textile industry associated with the production of biomass. The use of wastewater from textile production for the production of peroxidases results in an unprecedented circular process.