Postharvest Biology and Technology最新文献

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Corrigendum to ''Tyr-Asp enhances chilling tolerance in banana fruit by modulating redox homeostasis'' [Postharvest Biol. Technol., vol., 222, April 2025, 113338]
IF 6.4 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-01-11 DOI: 10.1016/j.postharvbio.2025.113395
Yanxuan Zhu , Yiejie Zhou , Zhiwei Li , Ruimin Zhong , Yueming Jiang , Guoxiang Jiang , Xinguo Su
{"title":"Corrigendum to ''Tyr-Asp enhances chilling tolerance in banana fruit by modulating redox homeostasis'' [Postharvest Biol. Technol., vol., 222, April 2025, 113338]","authors":"Yanxuan Zhu , Yiejie Zhou , Zhiwei Li , Ruimin Zhong , Yueming Jiang , Guoxiang Jiang , Xinguo Su","doi":"10.1016/j.postharvbio.2025.113395","DOIUrl":"10.1016/j.postharvbio.2025.113395","url":null,"abstract":"","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113395"},"PeriodicalIF":6.4,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143342784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptomics and physiological indicators analyzed the delayed effect of carboxymethyl chitosan on lignification of okra during postharvest cold storage
IF 6.4 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-01-11 DOI: 10.1016/j.postharvbio.2025.113394
Lijuan Wei, Shunjiao Gao, Fan Li, Xinyue Zhang, Yue Peng, Yaowen Liu, Yiqin Zhang, Wen Qin
{"title":"Transcriptomics and physiological indicators analyzed the delayed effect of carboxymethyl chitosan on lignification of okra during postharvest cold storage","authors":"Lijuan Wei,&nbsp;Shunjiao Gao,&nbsp;Fan Li,&nbsp;Xinyue Zhang,&nbsp;Yue Peng,&nbsp;Yaowen Liu,&nbsp;Yiqin Zhang,&nbsp;Wen Qin","doi":"10.1016/j.postharvbio.2025.113394","DOIUrl":"10.1016/j.postharvbio.2025.113394","url":null,"abstract":"<div><div>Lignification is one of the key factors leading to postharvest quality deterioration of fruit and vegetables. In this study, the effects of carboxymethyl chitosan (CMCS) on nutritional characteristics, cell wall substances, key enzyme activities, transcriptome and related gene expression levels of okra during postharvest low temperature storage were investigated. The results showed that CMCS could maintain the nutritional quality of postharvest okra, delay the accumulation of rigid components (lignin, cellulose, hemicellulose and pectin) in cell wall, and inhibit the enzyme activities of phenylpropanoid pathway (PAL, 4CL, CAD and POD). Transcriptome data analysis and qRT-PCR results showed that CMCS down-regulated the expression levels of lignin synthesis-related genes (<em>AePAL, AeC4H, Ae4CL, AeCAD, AeCOMT, AePOD</em>). MYB is an important transcription factor (TF) regulating lignin accumulation. The results of gene promoter analysis in qRT-PCR showed that 14 genes contained multiple MYB cis-acting elements. Compared with CK, CMCS inhibited the expression of multiple upstream <em>AeMYBs</em>. Collectively, CMCS is a promising green preservative by inhibiting the metabolism of nutrients, the enzyme activities of the phenylpropanoid pathway, and the expression levels of related genes and TFs, thereby delaying the accumulation of lignin during postharvest cold storage of okra and maintaining postharvest quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113394"},"PeriodicalIF":6.4,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exogenous NaHS maintains storage quality of Prunus salicina ‘Wushan plum’ by regulating the antioxidant system, endogenous H2S, ethylene and NO metabolism
IF 6.4 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-01-10 DOI: 10.1016/j.postharvbio.2025.113391
Saiying Han , Ling Liu , Lilei Wang , Jin Han , Yeru Ai , Huali Wang , Kaifang Zeng , Jian Ming , Lili Deng
{"title":"Exogenous NaHS maintains storage quality of Prunus salicina ‘Wushan plum’ by regulating the antioxidant system, endogenous H2S, ethylene and NO metabolism","authors":"Saiying Han ,&nbsp;Ling Liu ,&nbsp;Lilei Wang ,&nbsp;Jin Han ,&nbsp;Yeru Ai ,&nbsp;Huali Wang ,&nbsp;Kaifang Zeng ,&nbsp;Jian Ming ,&nbsp;Lili Deng","doi":"10.1016/j.postharvbio.2025.113391","DOIUrl":"10.1016/j.postharvbio.2025.113391","url":null,"abstract":"<div><div><em>Prunus salicina</em> ‘Wushan plum’ is a climacteric fruit, which rapidly ripens under the action of ethylene after harvesting, resulting in quality deterioration that seriously restricts the sustainable development of the industry. In order to examine the potential role of sodium hydrosulfide (NaHS, the donor of exogenous H<sub>2</sub>S) in slowing down the ripening and senescence process of plum fruit, the changes in storage quality, antioxidant capacity, endogenous signals and gene expression levels were evaluated during storage at 25 °C for twelve days. The results indicated that NaHS treatment delayed the coloration of plum fruit and maintained higher firmness, titratable acid content (TA) and marketable fruit rate. Treatment with NaHS increased the activities of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR) and ascorbate peroxidase (APX); promoted the accumulation of total phenols (TP) and ascorbic acid (AsA), and inhibited the increase in reactive oxygen species (ROS), malondialdehyde (MDA) and lipoxygenase (LOX) activity, which in turn alleviated oxidative damage. H<sub>2</sub>S treatment down-regulated the expression of <em>PsACO1</em> and <em>PsACS1</em> and inhibited the production of ethylene; up-regulated the expression of <em>PsSiR</em>, <em>PsSAT</em>, <em>PsOASTL,</em> and <em>PsLCD</em>, which in turn elevated the activities of sulfite reductase (SiR), serine acetyltransferase (SAT), O-acetylserine cleavage lyase (OASTL), and L-cysteine desulfurylase (LCD), and facilitated the accumulation of the endogenous H<sub>2</sub>S content; up-regulated the expression of L-Arginine synthesis-related genes and <em>PsNOA</em>, which increased the content of L-Arginine as well as the activity of nitric oxide synthase (NOS) and facilitated the synthesis of nitric oxide (NO). The above results indicated that NaHS could improve the antioxidant system, endogenous H<sub>2</sub>S, ethylene, and NO metabolism of plum, thus maintaining the storage quality of the fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113391"},"PeriodicalIF":6.4,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Revealing cold plasma-mediated changes in wolfberry wax and related gene expression during storage
IF 6.4 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-01-10 DOI: 10.1016/j.postharvbio.2025.113390
Yuhang Du , Kunfeng Liu , Shaofeng Yuan , Hang Yu , Yahui Guo , Yuliang Cheng , Weirong Yao
{"title":"Revealing cold plasma-mediated changes in wolfberry wax and related gene expression during storage","authors":"Yuhang Du ,&nbsp;Kunfeng Liu ,&nbsp;Shaofeng Yuan ,&nbsp;Hang Yu ,&nbsp;Yahui Guo ,&nbsp;Yuliang Cheng ,&nbsp;Weirong Yao","doi":"10.1016/j.postharvbio.2025.113390","DOIUrl":"10.1016/j.postharvbio.2025.113390","url":null,"abstract":"<div><div>Cold plasma (CP) is promising for postharvest storage and processing of fruit. However, as the first surface material that CP interacts with, the effects of CP on the cuticular wax of fruit remain unclear. This study aimed to elucidate how direct treatment with dielectric barrier discharge plasma, at varying power levels, affects the structure, composition, and biosynthesis of wolfberry wax, and its implications on postharvest storage and drying. Our findings reveal a power-dependent response. High-power CP (&gt;20 W) increased wax hydrophilicity, accelerating drying but potentially compromising storage stability due to increased water loss. Conversely, low-power CP (&lt;20 W) caused minimal initial damage, with partial recovery observed during storage. Notably, 10 W treatment even promoted alkane accumulation after two days, potentially enhancing storage quality. High-power CP (40 W) induced changes in wax composition, transforming alkanes into alcohols, ketones, fatty acids, and lipids, while reducing long-chain alkanes and fatty acids, and increasing short-chain alcohols and esters. These changes were linked to the downregulation of genes involved in alkane hydroxylation, wax precursor synthesis, and the acyl reduction pathway. In contrast, 10 W treatment exhibited a milder and more targeted impact on specific wax biosynthesis steps. These findings provide insights into CP's effects on waxes, guiding CP processing for wolfberries and other fruit to enhance postharvest quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113390"},"PeriodicalIF":6.4,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional analysis of potential fumiquinazoline biosynthetic gene clusters in Penicillium digitatum of citrus
IF 6.4 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-01-10 DOI: 10.1016/j.postharvbio.2025.113393
Shuqi Liu , Yongqing Lu , Yuqing Wang , Wei Fang , Chao-an Long
{"title":"Functional analysis of potential fumiquinazoline biosynthetic gene clusters in Penicillium digitatum of citrus","authors":"Shuqi Liu ,&nbsp;Yongqing Lu ,&nbsp;Yuqing Wang ,&nbsp;Wei Fang ,&nbsp;Chao-an Long","doi":"10.1016/j.postharvbio.2025.113393","DOIUrl":"10.1016/j.postharvbio.2025.113393","url":null,"abstract":"<div><div><em>Penicillium digitatum</em> is a pathogenic fungus responsible for the postharvest green mold of citrus, leading to significant economic losses in the citrus industry. Filamentous fungi have a rich secondary metabolic network, and their products have important applications in medicine. Several secondary metabolites (tryptoquialanines, fumiquinazolines, and so on) have been identified in <em>P. digitatum</em>. However, the molecular networks regulating the biosynthesis of secondary metabolites and their gene functions remain to be investigated. For functional analysis of the genes, a homologous recombination knockout strategy was used to generate knockout mutants of seven genes putative to be involved in the fumiquinazoline (FQ) biosynthesis pathway in <em>Penicillum digitatum</em>. LC-MS analysis revealed that the FQ biosynthetic gene clusters mainly regulated tryptoquialanine biosynthesis in <em>P. digitatum.</em> Virulence assessment experiments with knockout mutants displayed that compared with that in the wild type, the onset of green mold was delayed in all the citrus groups inoculated with the seven mutants, revealing that the involvement of the FQ biosynthetic gene clusters positively regulates the virulence of <em>P. digitatum</em>. Furthermore, the survival of the knockout mutants was greatly decreased under ultraviolet (UV-C) radiation, indicating that this gene cluster participated in the UV-C sensitivity of <em>P. digitatum.</em> The present study also revealed that genes in FQ biosynthetic gene clusters regulate low-temperature stress resistance and KCl stress resistance. In summary, this study determined that the FQ biosynthetic gene clusters regulate the biosynthesis of SMs and respond to multiple biological processes in <em>P. digitatum</em>.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113393"},"PeriodicalIF":6.4,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcription factor AcAGL9 and AcBEH1 synergistically regulate carotenoid biosynthesis during pineapple fruit ripening
IF 6.4 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-01-09 DOI: 10.1016/j.postharvbio.2024.113385
Mengting Liu , Ting Li , Rufang Deng , Zhengke Zhang , Yijie Zhou , Xuewu Duan , Yueming Jiang , Guoxiang Jiang
{"title":"Transcription factor AcAGL9 and AcBEH1 synergistically regulate carotenoid biosynthesis during pineapple fruit ripening","authors":"Mengting Liu ,&nbsp;Ting Li ,&nbsp;Rufang Deng ,&nbsp;Zhengke Zhang ,&nbsp;Yijie Zhou ,&nbsp;Xuewu Duan ,&nbsp;Yueming Jiang ,&nbsp;Guoxiang Jiang","doi":"10.1016/j.postharvbio.2024.113385","DOIUrl":"10.1016/j.postharvbio.2024.113385","url":null,"abstract":"<div><div>Pineapple fruit ripening is closely associated with carotenoid biosynthesis, which is crucial for color development and contributes to the fruit's overall quality. However, the transcriptional mechanisms driving carotenoid biosynthesis in pineapple remain largely unexplored. In this study, we identify a MADS-box transcription factor, AcAGL9, as a positive regulator of carotenoid biosynthesis in pineapple. Biochemical analyses demonstrate that AcAGL9 activates the expression of carotenogenic genes (<em>AcPSY</em>, <em>AcPDS</em>, <em>AcCRTISO</em>, and <em>AcLCYB</em>) by directly binding to CArG-elements in their promoters. Additionally, AcAGL9 physically interacts with the positive regulator AcBEH1, synergistically enhancing the expression of these carotenogenic genes. Silencing <em>AcAGL9</em> or <em>AcBEH1</em> in pineapple fruit reduces the expression of carotenoid biosynthesis genes and inhibits carotenoid accumulation, while overexpression has the opposite effect. Co-silencing or co-overexpression of both <em>AcAGL9</em> and <em>AcBEH1</em> produces an even more pronounced impact. Collectively, our study highlights the critical role of the AcAGL9-AcBEH1 module in regulating carotenoid accumulation in pineapple fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113385"},"PeriodicalIF":6.4,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenylalanine enhances the efficiency of sodium dehydroacetate to control citrus fruit decay by stimulating reactive oxygen metabolism and phenylpropanoid pathway
IF 6.4 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-01-08 DOI: 10.1016/j.postharvbio.2025.113392
Zhaoguo Liu , Okwong Oketch Reymick , Zhao Feng , Bin Duan , Nengguo Tao
{"title":"Phenylalanine enhances the efficiency of sodium dehydroacetate to control citrus fruit decay by stimulating reactive oxygen metabolism and phenylpropanoid pathway","authors":"Zhaoguo Liu ,&nbsp;Okwong Oketch Reymick ,&nbsp;Zhao Feng ,&nbsp;Bin Duan ,&nbsp;Nengguo Tao","doi":"10.1016/j.postharvbio.2025.113392","DOIUrl":"10.1016/j.postharvbio.2025.113392","url":null,"abstract":"<div><div>Postharvest citrus fruit are susceptible to decay and quality deterioration during storage. In this study, the addition of phenylalanine (Phe) to sodium dehydroacetate (SD) treatment on citrus fruit decay was investigated. Results showed that the addition of 1 g L<sup>−1</sup> Phe significantly improved the controlling efficiency of SD to citrus green mold incidence and natural rot diseases, which displayed a comparable level to that of imazalil and effectively maintained the fruit quality parameters including weight loss, titratable acid, vitamin C, and firmness. Meanwhile, addition of Phe could drastically reduce the contents of hydrogen peroxide, superoxide anion, and malondialdehyde by increasing the activities of catalase, superoxide dismutase, peroxidase, glutathione reductase, dehydroascorbate reductase, monodehydroascorbate reductase, ascorbate peroxidase and the levels of glutathione and ascorbic acid. It could also improve the activities of phenylalanine ammonia-lyase, cinnamate-4-hydroxylase and 4-coumarate coenzyme A ligase and the phenolic and flavonoid levels. These results suggested that Phe could improve the controlling efficiency of SD to citrus fruit decay through stimulation of reactive oxygen species (ROS) metabolism and phenylpropanoid pathway.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113392"},"PeriodicalIF":6.4,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prediction of chilling injury and key indicator parameters in sweet potatoes using VNIR spectroscopy
IF 6.4 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-01-08 DOI: 10.1016/j.postharvbio.2025.113389
Jong Hwan Lee , DoSu Park , Su Ho Tae , Se Min Chang , Min Woo Baek , Shimeles Tilahun , Cheon Soon Jeong
{"title":"Prediction of chilling injury and key indicator parameters in sweet potatoes using VNIR spectroscopy","authors":"Jong Hwan Lee ,&nbsp;DoSu Park ,&nbsp;Su Ho Tae ,&nbsp;Se Min Chang ,&nbsp;Min Woo Baek ,&nbsp;Shimeles Tilahun ,&nbsp;Cheon Soon Jeong","doi":"10.1016/j.postharvbio.2025.113389","DOIUrl":"10.1016/j.postharvbio.2025.113389","url":null,"abstract":"<div><div>Chilling injury (CI) in sweet potatoes, which leads to the undesirable accumulation of reducing sugars and renders them unsuitable for processing, is usually assessed through destructive analysis. This study was conducted to investigate the possibility of nondestructive estimation of CI and indicative parameters in intact sweet potatoes. CI indices were developed to estimate CI in ‘Beni haruka’ and ‘Hogammi’ sweet potato cultivars using firmness, total soluble solids (TSS), and malondialdehyde (MDA) as key indicators. Then, visible/near-infrared (VNIR) spectra in interactance mode, along with actual measurements of firmness, TSS, MDA, and CI index, were collected from 160 roots of each cultivar stored at 4 ℃ for 30 days. The data were categorized into calibration, cross-validation, and prediction sets, with preprocessing applied to optimize prediction models. After evaluating the predictive power of raw spectra with spectra preprocessed using various methods such as standard normal variate (SNV), multiplicative scattering correction (MSC), Savitzky-Golay 1st and 2nd order derivatives, partial least square regression (PLSR) was used to develop models correlating VNIR spectra with reference values of TSS, MDA, and the CI index. The models demonstrated promising results, and the regression coefficients for the prediction (R<sub>p</sub><sup>2</sup>) between the VNIR spectra and the measured values of TSS, MDA, and the CI index were 0.936, 0.902, and 0.812 for ‘Beni haruka’ and 0.847, 0.896, and 0.825 for ‘Hogammi’, respectively. The low standard error of prediction and bias values further indicated the model’s robustness, making these models fast and cost-efficient alternatives to traditional destructive methods for CI analysis. Further research on different cultivars could further improve model accuracy and reliability.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113389"},"PeriodicalIF":6.4,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Targeted and spatial metabolomics unveil how brassinolide enhances polyphenol and proline metabolism in cold-stressed jujube fruit
IF 6.4 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-01-07 DOI: 10.1016/j.postharvbio.2024.113386
Chenyu Niu , Ting Guo , Wenhui Xu , Yizhou Gao , Lingling Liu , Jie Liu , Zhaojun Ban , Zhengbao Wu , Yunhong Jiang
{"title":"Targeted and spatial metabolomics unveil how brassinolide enhances polyphenol and proline metabolism in cold-stressed jujube fruit","authors":"Chenyu Niu ,&nbsp;Ting Guo ,&nbsp;Wenhui Xu ,&nbsp;Yizhou Gao ,&nbsp;Lingling Liu ,&nbsp;Jie Liu ,&nbsp;Zhaojun Ban ,&nbsp;Zhengbao Wu ,&nbsp;Yunhong Jiang","doi":"10.1016/j.postharvbio.2024.113386","DOIUrl":"10.1016/j.postharvbio.2024.113386","url":null,"abstract":"<div><div>Brassinolide (BR) enhances cold tolerance in fruit and vegetables, yet the mechanisms through which BR prevents chilling injury in jujube fruit remain unclear. This study employed targeted and spatial metabolomic approaches to quantify the accumulation and distribution of key enzymes involved in the metabolism of lignin, phenolic compounds, and proline in jujube. Brassinolide treatment mitigated chilling injury in jujube fruit, preserving the ascorbic acid content, cell wall structure, firmness, and flavor of jujube fruit during cold storage. Brassinolide effectively inhibited increases in fruit malondialdehyde content and relative conductivity caused by chilling. In jujube fruit, BR downregulated genes involved in lignin synthesis, thereby slowing the rate of lignification. This finding was supported by a spatial metabolomics analysis which illustrated the diffusion of substances related to lignin metabolism from the outside to the inside of the fruit. Furthermore, BR promoted the accumulation of phenolics, leading to an even distribution of 4-methylumbelliferone, glutathione, and hesperetin throughout each fruit. By upregulating genes associated with proline synthesis, while inhibiting its degradation, BR treatment increased proline accumulation. Notably, glutathione was evenly distributed within BR-treated jujube fruit, whereas arginine was concentrated in the outer pulp. These findings lay the groundwork for the use of exogenous brassinolide to alleviate cold-induced fruit lignification and preserve jujube fruit quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113386"},"PeriodicalIF":6.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sodium trans-2-hexenylate damages the mitochondrial function of Penicillium digitaum and plays a significant role in citrus fruit postharvest disease control
IF 6.4 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-01-07 DOI: 10.1016/j.postharvbio.2024.113387
Qiuli OuYang, MingChen Yang, Yonghua Zhang, Lu Li, Xiaoli Tan, Nengguo Tao
{"title":"Sodium trans-2-hexenylate damages the mitochondrial function of Penicillium digitaum and plays a significant role in citrus fruit postharvest disease control","authors":"Qiuli OuYang,&nbsp;MingChen Yang,&nbsp;Yonghua Zhang,&nbsp;Lu Li,&nbsp;Xiaoli Tan,&nbsp;Nengguo Tao","doi":"10.1016/j.postharvbio.2024.113387","DOIUrl":"10.1016/j.postharvbio.2024.113387","url":null,"abstract":"<div><div>Huge postharvest citrus infections are produced by <em>Penicillium digitatum</em>. The antifungal activity of sodium <em>trans</em>-2-hexenylate (STH) against the <em>P. digitatum</em> growth and possible mechanism were investigated. The minimal inhibitory and fungicidal concentrations of STH against <em>P. digitatum</em> were 0.4 and 0.8 g L<sup>–1</sup>, respectively. STH decreased the green mold incidence of Ponkan fruit without negatively impacting fruit quality. STH altered and distorted the external appearance of <em>P. digitatum</em> mycelia rather than the cell wall. Correspondingly, STH decreased the lipid and ergosterol levels while degrading the cell membrane integrity. Meanwhile, STH affected mitochondrial function by lowering the mitochondria membrane potential (MMP) and ATP levels, as well as malate dehydrogenase (MDH) and succinate dehydrogenase (SDH) activities. Furthermore, MMP damage occurred earlier than that of cell membrane damage. Our current study showed that STH may be an acceptable substitute to chemical fungicide in postharvest disease control in citrus fruit and its underlying inhibitory mechanism is attributed to its ability to impair the mitochondrial function of <em>P. digitatum</em>.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113387"},"PeriodicalIF":6.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143092659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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