Postharvest Biology and Technology最新文献

{"title":"Effects of exogenous strigolactones on tomato-fruit quality","authors":"Jiaying Wang ,&nbsp;Li Jin ,&nbsp;Wen Zhu ,&nbsp;Ning Jin ,&nbsp;Qin Sun ,&nbsp;Qian Zhang ,&nbsp;Wei Zhang ,&nbsp;Shuya Wang ,&nbsp;Yandong Xie ,&nbsp;Jian Lyu ,&nbsp;Jihua Yu","doi":"10.1016/j.postharvbio.2025.113461","DOIUrl":"10.1016/j.postharvbio.2025.113461","url":null,"abstract":"<div><div>The objective of this study was to investigate the effect of the synthetic analog of strigolactone, GR24 (SL), on the quality of tomato fruits. The surfaces of tomatoes (<em>Solanum lycopersicum</em> cv. Micro-Tom) fruits were treated with different concentrations of SL treatments. The following concentrations were used: 0 (CK), 0.1 (T1), 0.5 (T2), (T3), 2 (T4), and 3 μmol L⁻¹ strigolactone synthesis inhibitor (Tis108). The experiment was conducted over 3 d, from the 24th to the 26th day after the fruit setting (mature green fruit stage). The results demonstrated that, after 24 d of treatment, the soluble solids in the T3 treatment exhibited a notable increase of 13.48 % compared to the CK. In addition, the contents of soluble protein and vitamin C demonstrated significant elevations of 46.74 %, 35.04 %, 1.36 %, and 1.40 %, respectively, compared to the CK and Tis108 treatments. Accordingly, T3 treatment was identified as the optimal SL concentration for investigating the impact of SL treatment on sensory, nutritional, and flavor quality during fruit ripening. SL treatment upregulated the gene expression of <em>SlAI</em>, <em>SlNI</em>, <em>SlSPS</em>, and <em>SlSS</em> in the sucrose metabolic pathway. In addition, it promoted an increase in acid invertase (AI), neutral invertase (NI), sucrose synthase (SS), and sucrose phosphate synthase (SPS) activities; accelerated the hydrolysis of sucrose; promoted the accumulation of fructose and glucose; and increased the total sugar content of fruits. Concurrently, SL treatment resulted in a notable reduction in the total acid content of the fruit, accompanied by an increase in the sugar-acid ratio. E-nose analysis also showed that SL treatment increased the characteristic aroma of fruits. In addition, the total amino acid content of tomato fruits was markedly enhanced following the SL treatment. On the 24th day, the total amino acid content of the SL treatment significantly increased by 16.04 % and 8.93 % compared to the CK and Tis108 treatments, respectively. Furthermore, the concentrations of phenolic acids and flavonoids in SL-treated fruits were markedly elevated compared to those observed in the CK and Tis108 treatments. SL has a considerable impact on enhancing the sensory quality, nutritional value, and flavor profile of tomato fruits.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"224 ","pages":"Article 113461"},"PeriodicalIF":6.4,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycine betaine and MiWRKY53 enhance antioxidant capacity and disease resistance against Colletotrichum gloeosporioides in mango fruit","authors":"Xiaomin Niu ,&nbsp;Yuxin Hu ,&nbsp;Xin Wang ,&nbsp;Rui Li ,&nbsp;Wen Li ,&nbsp;Yuanzhi Shao","doi":"10.1016/j.postharvbio.2025.113464","DOIUrl":"10.1016/j.postharvbio.2025.113464","url":null,"abstract":"<div><div>Mangoes, a quintessential tropical fruit, are highly susceptible to senescence and spoilage caused by <em>Colletotrichum gloeosporioides</em> during postharvest storage. Glycine betaine (GB), a natural compound with broad applications in the food industry, was investigated for its effects on the postharvest physiology, quality characteristics, and antioxidant activity of “Tainong No.1” mangoes. This study further elucidated the regulatory role of MiWRKY53 in the GB-mediated response. Results revealed that 10 mM GB treatment significantly suppressed the increase in the disease index while preserving titratable acidity (TA) and ascorbic acid (Vc) contents compared to the control (<em>P</em> &lt; 0.05). GB treatment also reduced respiration rate, cell membrane permeability, and malondialdehyde (MDA) content, maintaining fruit firmness and delaying softening during storage. Moreover, GB treatment enhanced the activities of antioxidant enzymes, including phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL), chalcone isomerase (CHI), and catalase (CAT), which promoted the accumulation of antioxidant compounds such as total phenols, flavonoids, and lignin. An <em>in vivo</em> assay further demonstrated that GB disrupted fungal mycelium structure and inhibited spore germination in wounded mango fruit. Furthermore, GB treatment significantly upregulated the transcription levels of key genes, including <em>MiPAL</em>, <em>Mi4CL</em>, <em>MiCHI</em>, and <em>MiWRKY53</em>. <em>MiWRKY53</em> was identified as a WRKY transcription factor that shows close evolutionary similarity to <em>Pistacia vera</em> based on Neighbor-joining phylogenetic tree analysis. Functional assays revealed that transient overexpression of <em>MiWRKY53</em> enhanced CHI activity, reduced disease lesions, and maintained green pigmentation in mango discs and tobacco leaves. Conversely, virus-induced gene silence (VIGS) of <em>MiWRKY53</em> resulted in adverse effects. This study highlights the potential of 10 mM GB treatment to control postharvest diseases and extend the shelf life of mangoes. The findings provide practical insights into leveraging natural compounds and genetic regulation for improving postharvest mango fruit quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"224 ","pages":"Article 113464"},"PeriodicalIF":6.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chondroitin sulfate alleviates leaf senescence of Chinese flowering cabbage by modulating homeostasis of ROS and chlorophyll catabolism during storage","authors":"Pengfei Du ,&nbsp;Yaqi Li ,&nbsp;Yulong Chen ,&nbsp;Hua Huang ,&nbsp;Fanwei Dai ,&nbsp;Jianye Chen ,&nbsp;Mingqiang Ye ,&nbsp;Feiping Chen ,&nbsp;Yingwei Qi ,&nbsp;Zheng Luo ,&nbsp;Ling Wang","doi":"10.1016/j.postharvbio.2025.113463","DOIUrl":"10.1016/j.postharvbio.2025.113463","url":null,"abstract":"<div><div>Chinese flowering cabbage is a nutrient abundance and popular vegetable while being sensitive to leaf senescence after harvest. Herein, the application of chondroitin sulfate (CS), a glycosaminoglycan, to delay the postharvest leaf senescence of Chinese flowering cabbage were comprehensively investigated. CS treatment could effectively inhibit the changes of yellowing index, chromaticity values of <em>L*</em> , <em>C*</em> , hue angle, and decrease of chlorophyll content in leaf of Chinese flowering cabbage. The expression levels of chlorophyll degradation catabolic genes <em>BrNYC1</em>, <em>BrSGR1</em>, <em>BrSGR2</em>, <em>BrPPH</em>, <em>BrPAO</em>, and <em>BrRCCR</em>, and representative senescence associated genes <em>BrSAG12</em> and <em>BrSAG21</em> were evidently repressed by CS as compared to control. The treatment of CS slowed down the production of the superoxide radical (O₂^-.), hydrogen peroxide (H₂O₂) and malondialdehyde. The activity enzyme of nicotinamide adenine dinucleotide phosphate oxidase (NOX), and the transcript abundance of respiratory burst oxidase homologues (<em>RBOH</em>) genes of <em>BrRbohB</em>, <em>BrRbohC</em>, <em>BrRbohD, BrRbohD2</em> and <em>BrRbohE</em> were retarded. Additionally, the activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) was maintained, and their corresponding genes <em>BrCAT3, BrCu/Zn-SOD1</em>, <em>BrPOD42</em>, and <em>BrAPX5</em> were up-regulated. These findings indicate that application of CS might potentially delay the leaf senescence by modulating ROS homeostasis and chlorophyll degradation for Chinese flowering cabbage during storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"224 ","pages":"Article 113463"},"PeriodicalIF":6.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined analyses of metabolomics and transcriptomics reveal the mechanism of DNP and ATP regulating the metabolisms of organic acids in pulp of longan fruit during storage","authors":"Zhe Li ,&nbsp;Hetong Lin ,&nbsp;Lingzhen Zeng ,&nbsp;Shucheng Li ,&nbsp;Yueying Sang ,&nbsp;Zhongqi Fan ,&nbsp;Yihui Chen ,&nbsp;Yifen Lin ,&nbsp;Wangjin Lu ,&nbsp;Hui Wang","doi":"10.1016/j.postharvbio.2025.113455","DOIUrl":"10.1016/j.postharvbio.2025.113455","url":null,"abstract":"<div><div>The quality deterioration in pulp of postharvest longan fruit is related to the increase in acidity during storage. However, the main organic acids and their regulatory mechanisms are still unrevealed. This study explored the impacts of 2,4-dinitrophenol (DNP) and adenosine triphosphate (ATP) treatments on the energy level and their influences on titratable acid (TA) contents in longan pulp. Compared with the control samples, lower levels of pulp energy charge, ATP and total soluble solids (TSS), but higher fruit respiration rate, pulp breakdown index and TA content were found in DNP-treated longans. Moreover, higher levels of four organic acids (fumaric acid, lactate, succinic acid, and oxaloacetate) were presented in DNP-treated samples through the analysis of targeted metabolome. However, the ATP treatment showed the opposite effects. The transcriptome analysis showed that differentially expressed genes (DEGs) among the three treatment groups were predominantly enriched in respiration metabolism pathways, including glycolysis, gluconeogenesis and tricarboxylic acid (TCA) cycle. In addition, weighted gene co-expression network analysis (WGCNA) identified four genes (<em>DlSDH</em>, <em>DlLDH</em>, <em>DlSCS,</em> and <em>DlMDH</em>) associated with the aforementioned metabolic pathways. RT-qPCR results exhibited that, during storage, these four genes in longan pulp were upregulated by DNP treatment and downregulated by ATP treatment. Therefore, energy deficit could promote the expression of <em>DlSDH</em>, <em>DlLDH</em>, <em>DlSCS,</em> and <em>DlMDH</em>, enhancing the metabolisms of glycolysis, gluconeogenesis, and TCA cycle, thereby accelerating the increase of organic acids in pulp of fresh longan during storage. This work revealed the main organic acids, the metabolic pathways, and the key genes involved in the increased acidity in pulp of postharvest longan fruit, as well as expanded our knowledge of energy regulating longan fruit quality and its relationship with the metabolism of organic acids.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"224 ","pages":"Article 113455"},"PeriodicalIF":6.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytic acid pretreatment potentiates the biocontrol efficacy of Meyerozyma caribbica against Aspergillus carbonarius infection in table grapes: Insights from physiological and transcriptomic analyses","authors":"Joice Meshi Foku,&nbsp;Michael Ackah,&nbsp;Ji Qihao,&nbsp;Qiya Yang,&nbsp;Hongyin Zhang","doi":"10.1016/j.postharvbio.2025.113465","DOIUrl":"10.1016/j.postharvbio.2025.113465","url":null,"abstract":"<div><div><em>Meyerozyma caribbica,</em> previously known as <em>Candida fermentati</em>, is a sporogenous yeast belonging to the <em>Meyerozyma guilliermondii</em> species complex. It is present in various environmental niches owing to its diverse physiological and metabolic capacities that promote adaptation and survival. This study used phytic acid (PA) pretreated <em>M. caribbica</em> to control sour rot caused by <em>Aspergillus carbonarius</em> in grape berries via exploring the physiological and molecular mechanisms involved. Table grapes were treated with either sterile distilled water (SDW) as a control (CK), PA only (E10), <em>M. caribbica</em> alone (Y), or <em>M. caribbica</em> enhanced with PA (YE10) for four days after inducing decay with <em>A. carbonarius</em>. Pretreatment of <em>M. caribbica</em> with 10 µmol/mL of PA significantly reduced the incidence of decay caused by <em>A. carbonarius</em> by 64.11 %, compared to a 25 % reduction by <em>M. caribbica</em> alone, after four days of storage at 20°C. Additionally, <em>M. caribbica</em> pretreatment with 10µmol/mL PA effectively suppressed mycelia growth and spore germination from <em>A. carbonarius</em> both <em>in vitro</em> and <em>in vivo</em>. This treatment boosted the activity of defense-related enzymes like polyphenol oxidase (PPO), chitinase (CHI), phenylalanine ammonia-lyase (PAL), and β-1,3-glucanase (GLU). The gene expression levels of these enzymes were elevated in fruits treated with PA-enhanced <em>M. caribbica</em>. Based on transcriptome analysis, 111 genes were differentially expressed (DEGs), 59 up-regulated and 52 down-regulated, and enriched in pathways associated with antioxidant metabolites implicated in resistance to diseases, the brassinosteroid biosynthetic pathway, and others. This comprehensive method using physiological and transcriptomic techniques to study the management of <em>A. carbonarius</em> rot in table grapes by <em>M. caribbica</em> enhanced with PA may offer an alternative strategy and help manage postharvest diseases of table grapes.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"224 ","pages":"Article 113465"},"PeriodicalIF":6.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peach fruit quality on the fresh market: A long-term evaluation series from Northern Italy is pushing the overturn of commercial standards within the mass-supply chain","authors":"Irina Baccichet ,&nbsp;Francesca Patuelli ,&nbsp;Benedetta Pozzetto ,&nbsp;Alessandro Giulio Tagliabue ,&nbsp;Cassia da Silva Linge ,&nbsp;Gianluca Biffi ,&nbsp;Elisa Calastri ,&nbsp;Remo Chiozzotto ,&nbsp;Daniele Bassi ,&nbsp;Paola Sgarbi ,&nbsp;Marco Cirilli","doi":"10.1016/j.postharvbio.2025.113460","DOIUrl":"10.1016/j.postharvbio.2025.113460","url":null,"abstract":"<div><div>Commercial peach fruit quality standards mainly rely on visual and fruit size attributes, often unpredictable or unrelated to organoleptic pleasantness. Hence, surveys on consumers’ continuously reports a poor fresh internal quality of peaches as the main cause of a widespread disaffection. In addition, the consistent adoption of similar selection criteria among main breeding programs hampers a marked improvement of enhanced flavour. This eight-years study reported the quality analysis of 52 peach cultivars sold across several mass-supply chain channels (the so-called ‘Organized Large-Scale Retail Distribution’, GDO), describing the current landscape of marketed peaches in Italy. In addition to the evaluation of external and internal quality parameters, a group of experienced panelists performed a sensory evaluation at commercial ripening (‘ready-to-buy’ stage) and after shelf-life (‘ready-to-eat’) to complement analytic determination and further investigate its relationship with human perception. Overall, soluble solid content (SSC) resulted inconsistent and generally low over the years of assessment, particularly in early and mid-season cultivars. This evidence negatively impacted panelists’ pleasantness that, in turn, appeared strongly dependent on taste sensorial experience and, above all, on aroma and sweetness perception. In particular, aroma tended to be poorly perceived with SSCs below 12 %, while a significant superior preference occurred with SSC above 14 % and a BrimA index above 27. The almost negligible effect of shelf-life on SSC emphasized the largest effect of the pre-harvest conditions for this feature. Accordingly, this study endorses an impelling improvement of the agronomic practices currently adopted and, hopefully, a reorganization of the whole peach retailers’ distribution chain to really meet consumers’ preferences.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"224 ","pages":"Article 113460"},"PeriodicalIF":6.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the inhibition mechanism of 1-octene-3-ol combined with 3-heptene-2-one on Aspergillus flavus and its application in the preservation of wolfberries","authors":"Lijun Ling ,&nbsp;Rongxiu Mo ,&nbsp;Wenyue Zhang ,&nbsp;Yijuan Jiang ,&nbsp;Fanjin Kong ,&nbsp;Lijun Feng ,&nbsp;Yao Li ,&nbsp;Rui Yue ,&nbsp;Yongpeng Zhou","doi":"10.1016/j.postharvbio.2025.113462","DOIUrl":"10.1016/j.postharvbio.2025.113462","url":null,"abstract":"<div><div>The antifungal activity of six microbial-derived volatile organic compounds (VOCs) [ 3-hepten-2-one (Hep), cis-3-octen-1-ol, 3-methylvalerate, isovalerate, 1-octen-3-ol (Oct), and 2-methylbutyrate] against <em>Aspergillus flavus</em> was investigated by determining the minimum inhibitory concentration (MIC) and the fractional inhibitory concentration index (FICI). The results demonstrated that all 15 binary combinations of the VOCs presented additive effects against <em>A. flavus</em>. Among them, the combination of Hep and Oct, refers to C_OH, with concentrations of 2 μL L⁻¹ and 2.5 μL L⁻¹, respectively, displayed the most potent antifungal effect with the lowest concentration, in contrast to the individual MIC of Hep or Oct, which were reduced to 1/8 and 1/2. C_OH can induce membrane lipid peroxidation, enhance cell membrane permeability, and cause leakage of nucleic acids and proteins within the cell, and 1-octen-3-ol might play a leading role. Finally, applying C_OH at a concentration of 1/2 MIC_Oct + 1/4 MIC_Hep could effectively restrain the spread of <em>A. flavus</em> on wolfberries and exhibit excellent preservation effects. This study validates the antifungal effect of C_OH and may serve as a natural alternative to antimicrobial agents for post-harvest agricultural products.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113462"},"PeriodicalIF":6.4,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperspectral imaging with multivariate analysis for detection of exterior flaws for quality evaluation of apples and pears","authors":"Tanjima Akter ,&nbsp;Mohammad Akbar Faqeerzada ,&nbsp;Yena Kim ,&nbsp;Muhammad Fahri Reza Pahlawan ,&nbsp;Umuhoza Aline ,&nbsp;Haeun Kim ,&nbsp;Hangi Kim ,&nbsp;Byoung-Kwan Cho","doi":"10.1016/j.postharvbio.2025.113453","DOIUrl":"10.1016/j.postharvbio.2025.113453","url":null,"abstract":"<div><div>Hyperspectral imaging (HSI) has emerged as a highly effective, non-destructive technique for detecting external and subsurface defects in agricultural products, offering rapid and accurate quality assessment. This study employed an advanced HSI system operating in the 400–2500 nm range, encompassing visible near-infrared (VNIR) and short-wave infrared (SWIR) wavelengths, to identify multiple defects in apples and pears. Specifically, this research focused on three key defect types, bruises, scars, and diseases, while addressing challenges related to defect size, shape, and severity variations. To enhance the spectral variability and improve detection accuracy, apples and pears were manually bruised in varying sizes, simulating both the early and advanced stages of bruising. These samples were analyzed at regular intervals over two days, capturing the progression of defect characteristics. This process yielded over 8300 spectral data points from carefully selected regions of interest (ROIs), providing a comprehensive dataset for analysis. Three multivariate models, linear discriminant analysis (LDA), support vector machines (SVM), and partial least squares discriminant analysis (PLS-DA), were employed to classify normal and defective fruit. Among these, the PLS-DA model demonstrated the highest performance, achieving validation classification accuracies of 97.5 % for apples and 100 % for pears in the VNIR range and 98 % for apples and 99.9 % for pears in the SWIR range. Moreover, successive projection algorithms (SPA) were employed for optimal band selection, and the obtained SPA beta coefficients were derived from the high-performing models and generated chemical visualization maps, enabling precise localization and detailed characterization of defects. By combining rapid and accurate defect identification with comprehensive flaw characterization, this study provides a practical and efficient framework for industrial post-harvest quality control, ultimately enhancing high-value agricultural products' marketability and storage stability.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113453"},"PeriodicalIF":6.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative transcriptomic and metabolomic analyses reveal the mechanisms behind acidity differences in the pulp of fresh longan cv. ‘Fuyan’ and ‘Dongbi’ during storage","authors":"Lingzhen Zeng ,&nbsp;Yueying Sang ,&nbsp;Shucheng Li ,&nbsp;Mengshi Lin ,&nbsp;Yifen Lin ,&nbsp;Hui Wang ,&nbsp;Yihui Chen ,&nbsp;Wangjin Lu ,&nbsp;Zhongqi Fan ,&nbsp;Hetong Lin","doi":"10.1016/j.postharvbio.2025.113447","DOIUrl":"10.1016/j.postharvbio.2025.113447","url":null,"abstract":"<div><div>To understand the underlying mechanism of the organic acid accumulation of postharvest longan fruit, integrative analyses of transcriptome and metabolome were performed in the fruit of two longan varieties (‘Fuyan’ and ‘Dongbi’) with different storability. Compared with ‘Dongbi’ longan, the ‘Fuyan’ cultivar exhibited higher fruit respiration rate, pulp breakdown index, and TA content, but a lower TSS content during storage. Targeted metabolomic analysis revealed that, in comparison to ‘Dongbi’ longan, during storage, ‘Fuyan’ longan accumulated higher levels of four organic acids, including 3-phenyllactic acid, lactate, succinic acid, and oxaloacetate. Transcriptomic analysis showed that DEGs between ‘Fuyan’ and ‘Dongbi’ longans were primarily enriched in respiration metabolism pathways, including glycolysis, gluconeogenesis, and the TCA cycle. Additionally, WGCNA identified hub genes involved in these metabolic pathways. RT-qPCR analysis indicated that the expression of related genes (<em>DlLDH</em>, <em>DlACL</em>, <em>DlPEPC</em>, <em>DlMDH</em>, <em>DlAST</em> and <em>DlSCS</em>) was upregulated in ‘Fuyan’ longan compared with ‘Dongbi’ longan during storage. These findings suggest that the increase in longan pulp acidity is attributed to organic acid accumulation and an enhanced respiration metabolism, resulting in the poor storability of ‘Fuyan’ longan during storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113447"},"PeriodicalIF":6.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SlNAC1 mediates MeSA application-promoted cold resistance by improving transcription of SlNRX2 in tomato fruit","authors":"Caifeng Jiao ,&nbsp;Jing Sun","doi":"10.1016/j.postharvbio.2025.113459","DOIUrl":"10.1016/j.postharvbio.2025.113459","url":null,"abstract":"<div><div>Refrigeration process is often regarded as a traditional practice utilized to conserve nutritive value in postharvest horticultural products. As a result of susceptibility to low temperature environment, tomato fruit exhibit the development of chilling injury (CI) during refrigerated preservation, ultimately constraining marketability. Methyl salicylate (MeSA) application attenuated the CI in tomatoes. The MeSA-dipped fruit possessed the compromised reactive oxygen species (ROS) generation. MeSA application was conducive to improve the transcription level of <em>SlNAC1.</em> The <em>SlNAC1</em>-OE fruit displayed the increase in cold resistance, accompanied by the diminished amount of ROS. In contrast, the lower cold resistance and larger amount of ROS were observed in the <em>SlNAC1</em>-silenced fruit. MeSA application conferred the boosted transcription level of nucleoredoxin 2 (<em>SlNRX2</em>). Moreover, the transcription abundances of <em>SlNRX2</em> increased by overexpression of <em>SlNAC1</em>, but decreased by RNAi of <em>SlNAC1</em>. It was substantiated that SlNAC1 transcriptionally promoted the <em>SlNRX2</em> expression by targeting the NAC binding motif in its promoter region afterwards. Collectively, SlNAC1 was involved in the MeSA application-weakened sensitivity to low temperature stress by transactivating <em>SlNRX2</em> in tomato fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113459"},"PeriodicalIF":6.4,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}