硫酸软骨素通过调节贮藏过程中活性氧和叶绿素分解代谢的稳态来缓解白菜叶片衰老

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-02-20 DOI:10.1016/j.postharvbio.2025.113463

Pengfei Du , Yaqi Li , Yulong Chen , Hua Huang , Fanwei Dai , Jianye Chen , Mingqiang Ye , Feiping Chen , Yingwei Qi , Zheng Luo , Ling Wang

{"title":"硫酸软骨素通过调节贮藏过程中活性氧和叶绿素分解代谢的稳态来缓解白菜叶片衰老","authors":"Pengfei Du , Yaqi Li , Yulong Chen , Hua Huang , Fanwei Dai , Jianye Chen , Mingqiang Ye , Feiping Chen , Yingwei Qi , Zheng Luo , Ling Wang","doi":"10.1016/j.postharvbio.2025.113463","DOIUrl":null,"url":null,"abstract":"<div><div>Chinese flowering cabbage is a nutrient abundance and popular vegetable while being sensitive to leaf senescence after harvest. Herein, the application of chondroitin sulfate (CS), a glycosaminoglycan, to delay the postharvest leaf senescence of Chinese flowering cabbage were comprehensively investigated. CS treatment could effectively inhibit the changes of yellowing index, chromaticity values of L* , C* , hue angle, and decrease of chlorophyll content in leaf of Chinese flowering cabbage. The expression levels of chlorophyll degradation catabolic genes BrNYC1, BrSGR1, BrSGR2, BrPPH, BrPAO, and BrRCCR, and representative senescence associated genes BrSAG12 and BrSAG21 were evidently repressed by CS as compared to control. The treatment of CS slowed down the production of the superoxide radical (O2-.), hydrogen peroxide (H2O2) and malondialdehyde. The activity enzyme of nicotinamide adenine dinucleotide phosphate oxidase (NOX), and the transcript abundance of respiratory burst oxidase homologues (RBOH) genes of BrRbohB, BrRbohC, BrRbohD, BrRbohD2 and BrRbohE were retarded. Additionally, the activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) was maintained, and their corresponding genes BrCAT3, BrCu/Zn-SOD1, BrPOD42, and BrAPX5 were up-regulated. These findings indicate that application of CS might potentially delay the leaf senescence by modulating ROS homeostasis and chlorophyll degradation for Chinese flowering cabbage during storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"224 ","pages":"Article 113463"},"PeriodicalIF":6.8000,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chondroitin sulfate alleviates leaf senescence of Chinese flowering cabbage by modulating homeostasis of ROS and chlorophyll catabolism during storage\",\"authors\":\"Pengfei Du , Yaqi Li , Yulong Chen , Hua Huang , Fanwei Dai , Jianye Chen , Mingqiang Ye , Feiping Chen , Yingwei Qi , Zheng Luo , Ling Wang\",\"doi\":\"10.1016/j.postharvbio.2025.113463\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Chinese flowering cabbage is a nutrient abundance and popular vegetable while being sensitive to leaf senescence after harvest. Herein, the application of chondroitin sulfate (CS), a glycosaminoglycan, to delay the postharvest leaf senescence of Chinese flowering cabbage were comprehensively investigated. CS treatment could effectively inhibit the changes of yellowing index, chromaticity values of L* , C* , hue angle, and decrease of chlorophyll content in leaf of Chinese flowering cabbage. The expression levels of chlorophyll degradation catabolic genes BrNYC1, BrSGR1, BrSGR2, BrPPH, BrPAO, and BrRCCR, and representative senescence associated genes BrSAG12 and BrSAG21 were evidently repressed by CS as compared to control. The treatment of CS slowed down the production of the superoxide radical (O2-.), hydrogen peroxide (H2O2) and malondialdehyde. The activity enzyme of nicotinamide adenine dinucleotide phosphate oxidase (NOX), and the transcript abundance of respiratory burst oxidase homologues (RBOH) genes of BrRbohB, BrRbohC, BrRbohD, BrRbohD2 and BrRbohE were retarded. Additionally, the activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) was maintained, and their corresponding genes BrCAT3, BrCu/Zn-SOD1, BrPOD42, and BrAPX5 were up-regulated. These findings indicate that application of CS might potentially delay the leaf senescence by modulating ROS homeostasis and chlorophyll degradation for Chinese flowering cabbage during storage.</div></div>\",\"PeriodicalId\":20328,\"journal\":{\"name\":\"Postharvest Biology and Technology\",\"volume\":\"224 \",\"pages\":\"Article 113463\"},\"PeriodicalIF\":6.8000,\"publicationDate\":\"2025-02-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Postharvest Biology and Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0925521425000754\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Postharvest Biology and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925521425000754","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

摘要

白菜是一种营养丰富、受欢迎的蔬菜，但收获后叶片衰老敏感。本文对糖胺聚糖硫酸软骨素（CS）在延缓白菜采后叶片衰老中的应用进行了全面研究。CS处理能有效抑制白菜叶片黄化指数、L* 、C* 色度值、色相角的变化和叶绿素含量的降低。叶绿素降解分解代谢基因BrNYC1、BrSGR1、BrSGR2、BrPPH、BrPAO和BrRCCR以及具有代表性的衰老相关基因BrSAG12和BrSAG21的表达水平被CS明显抑制。CS处理减缓了超氧自由基（O2-）、过氧化氢（H2O2）和丙二醛的生成。烟酰胺腺嘌呤二核苷酸磷酸氧化酶（NOX）活性酶和BrRbohB、BrRbohC、BrRbohD、BrRbohD2和BrRbohE呼吸爆发氧化酶同源物（RBOH）基因转录丰度降低。抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）活性维持正常，其对应基因BrCAT3、BrCu/Zn-SOD1、BrPOD42、BrAPX5表达上调。这些结果表明，施用CS可能通过调节白菜贮藏过程中活性氧稳态和叶绿素降解来延缓叶片衰老。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Chondroitin sulfate alleviates leaf senescence of Chinese flowering cabbage by modulating homeostasis of ROS and chlorophyll catabolism during storage

Chinese flowering cabbage is a nutrient abundance and popular vegetable while being sensitive to leaf senescence after harvest. Herein, the application of chondroitin sulfate (CS), a glycosaminoglycan, to delay the postharvest leaf senescence of Chinese flowering cabbage were comprehensively investigated. CS treatment could effectively inhibit the changes of yellowing index, chromaticity values of L* , C* , hue angle, and decrease of chlorophyll content in leaf of Chinese flowering cabbage. The expression levels of chlorophyll degradation catabolic genes BrNYC1, BrSGR1, BrSGR2, BrPPH, BrPAO, and BrRCCR, and representative senescence associated genes BrSAG12 and BrSAG21 were evidently repressed by CS as compared to control. The treatment of CS slowed down the production of the superoxide radical (O₂^-.), hydrogen peroxide (H₂O₂) and malondialdehyde. The activity enzyme of nicotinamide adenine dinucleotide phosphate oxidase (NOX), and the transcript abundance of respiratory burst oxidase homologues (RBOH) genes of BrRbohB, BrRbohC, BrRbohD, BrRbohD2 and BrRbohE were retarded. Additionally, the activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) was maintained, and their corresponding genes BrCAT3, BrCu/Zn-SOD1, BrPOD42, and BrAPX5 were up-regulated. These findings indicate that application of CS might potentially delay the leaf senescence by modulating ROS homeostasis and chlorophyll degradation for Chinese flowering cabbage during storage.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.