Postharvest Biology and Technology最新文献

{"title":"Transcriptomic and metabolomic analysis reveal the role of 5-aminolevulinic acid hydrochloride in suppressing postharvest decay development in sweet cherry","authors":"Shuaiqi Zhang ,&nbsp;Ranran Xu ,&nbsp;John B. Golding ,&nbsp;Lizhi Deng ,&nbsp;Jiahua Zhou ,&nbsp;Liya Liang ,&nbsp;Baogang Wang","doi":"10.1016/j.postharvbio.2024.113339","DOIUrl":"10.1016/j.postharvbio.2024.113339","url":null,"abstract":"<div><div>The development of fungal rots is one of the main limitations of the storage and marketing of sweet cherry fruit. Sweet cherry (<em>Prunus avium</em> L.) var ‘Xiangquan No.1’ fruit were treated with 1 g L⁻¹ 5-aminolevulinic acid (5-ALA) hydrochloride and stored at 20 °C for 12 d. Results showed that fruit treated with 5-ALA hydrochloride had significantly lowered postharvest decay. Transcriptomic and metabolomic analysis indicated that 1031 differentially accumulated metabolites (DAMs) and 6903 differentially expressed genes (DEGs) were detected between 5-ALA hydrochloride treatment and the control at 6 d. A weighted gene co-correlation network analysis was employed to evaluate the gene regulatory network of DEGs. The DEGs and DAMs were co-enriched in the phenylpropanoid biosynthesis and flavonoid biosynthesis pathways. Moreover, the 5-ALA hydrochloride treatment improved disease resistance of the fruit by activating genes related to salicylic acid signal transduction and down-regulation of the metabolite gibberellin in sweet cherry fruit. The multiple transcription factors, including NAC50, MYB62, LEP and WRKY40 were also activated by 5-ALA hydrochloride treatment. These results provided new ideas for research on the postharvest management and maintenance of sweet cherry fruit during storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"221 ","pages":"Article 113339"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preharvest methyl jasmonate maintain the shelf quality of kiwifruit after cold storage by regulating the antioxidant system","authors":"Xian Yang ,&nbsp;Na Liu ,&nbsp;Shuming Tan ,&nbsp;Yanqun Xu ,&nbsp;Zisheng Luo ,&nbsp;Guofang Xie","doi":"10.1016/j.postharvbio.2024.113335","DOIUrl":"10.1016/j.postharvbio.2024.113335","url":null,"abstract":"<div><div>Soft rot is an important postharvest disease of kiwifruit, and MeJA can induce kiwifruit to resist soft rot. However, the treatment timing of preharvest MeJA and the mechanism of inducing kiwifruit resistance are still unclear. In this study, we compared the effects of preharvest MeJA and postharvest MeJA on the shelf quality of kiwifruit after cold storage. We investigated the impact of soaked in MeJA for 10 s at 32 d after full bloom (DAFB) and/or sprayed with MeJA at 141 DAFB alone or simultaneously on the shelf quality of kiwifruit after cold storage, also the resistance of kiwifruit to <em>B. dothidea</em>. The results showed that both preharvest and postharvest MeJA inhibited the incidence of soft rot in postharvest kiwifruit by regulating antioxidant capacity, while preharvest MeJA was more effective compared to postharvest MeJA. Compared to the control, both preharvest MeJA at 32D or/and 141S kept the shelf quality of kiwifruit after cold storage, and the preharvest MeJA at 32D and 141S is optimal. Preharvest MeJA at 32D and 141S maintained the volatile aroma in kiwifruit during shelf quality by regulating the enzyme related to aroma metabolism, kept shelf quality by regulating the ROS homeostasis and glutathione-ascorbic acid (GSH-ASA) cycle, and improving the activities of disease-resistant enzymes. Further data from the kiwifruit inoculated with <em>B. dothidea</em> indicate that preharvest MeJA keeps the kiwifruit quality and induces its disease resistance during shelf life after cold storage by regulating the antioxidant system. Overall, these findings showed that preharvest MeJA kept the kiwifruit shelf quality after cold storage by regulating the antioxidant system.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"221 ","pages":"Article 113335"},"PeriodicalIF":6.4,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic and transcriptomic analyses provide insights into temperature and light regulated anthocyanin accumulation in flesh of ‘Furongli’ plum (Prunus salicina Lindl.)","authors":"Zhizhen Fang ,&nbsp;Kui Lin-Wang ,&nbsp;Yanjuan Lin ,&nbsp;Richard V. Espley","doi":"10.1016/j.postharvbio.2024.113326","DOIUrl":"10.1016/j.postharvbio.2024.113326","url":null,"abstract":"<div><div>Anthocyanins are health-promoting pigments that contribute to the quality of fruits. Anthocyanin biosynthesis is affected by light and temperature. However, there is no information available on the effects of light and temperature on anthocyanin accumulation in plum flesh. In this study, we investigated the effects of temperature and light on anthocyanin accumulation in postharvest ‘Furongli’ plum (<em>Prunus salicina</em> Lindl.) flesh and found that 20◦C promoted anthocyanin accumulation with or without light. However, no significant anthocyanin accumulation was detected in the flesh of plums treated with 30 ◦C/light. RNA-Seq analysis showed that the transcript levels of anthocyanin accumulation-related genes in the flesh were upregulated by 20◦C/light and 20◦C/dark treatments. Moreover, several genes involved in sucrose metabolism as well as plant hormone biosynthesis and signal transduction were transcriptionally correlated with anthocyanin accumulation. NAA treatment confirmed that auxin promoted anthocyanin accumulation in plum flesh. Transcription factors differentially regulated by temperature and light were identified. The anthocyanin MYB activator, PsMYB10.2, was only upregulated in the flesh of 20◦C/light and 20◦C/dark treated fruits, wheras the anthocyanin repressor, <em>PsMYB18,</em> was upregulated in flesh of fruits under all treatment conditions. The <em>PsMYB10.2</em> promoter was responsive to light, but not to temperature. Transient overexpression assays showed that <em>PsMYB18</em> inhibited <em>PsMYB10.2</em> and <em>PsbHLH3</em> induced anthocyanin accumlation in tobacco leaves. Dual luciferase assays further showed that <em>PsMYB18</em> repressed the activation activity of PsMYB10.2 on the promoters of <em>PsANS</em>, <em>PsUFGT</em> and <em>PsGST</em>. Yeast one-hybrid and dual luciferase assays showed PsMYB10.2 could bind to and activated the <em>PsMYB18</em> promoter. These results suggest that temperature and light modulate anthocyanin accumulation in the flesh of ‘Furongli’ plum by regulating the expression of structural genes involved in anthocyanin accumulation via the cooperation of anthocyanin regulators PsMYB10.2 and PsMYB18.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"221 ","pages":"Article 113326"},"PeriodicalIF":6.4,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142723021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential regulation of the phenylpropanoid pathway highly contributes to the susceptibility of chilling-induced necrotic peel disorders in cold-stored hardy kiwifruit","authors":"Hyowon Park ,&nbsp;Hyun Ji Eo ,&nbsp;Chul-Woo Kim ,&nbsp;Jane E. Stewart ,&nbsp;Uk Lee ,&nbsp;Jinwook Lee","doi":"10.1016/j.postharvbio.2024.113337","DOIUrl":"10.1016/j.postharvbio.2024.113337","url":null,"abstract":"<div><div>Fruit of hardy kiwifruit cultivars respond differently to chilling stress during cold storage. Therefore, this study aimed to evaluate the differential responses of fruit quality attributes, physiological disorders, and untargeted and targeted metabolites of two contrasting hardy kiwifruit cultivars, ‘Greenheart’ and ‘Daebo’ during cold storage. During cold storage, the ‘Daebo’ cultivar exhibited more severe symptoms of chilling injury, such as peel browning and peel pitting, compared to the ‘Greenheart’ cultivar. Untargeted and targeted metabolic analyses indicated that syringic acid, total phenolic compounds, total flavonoids, catechin, rutin, ferulic acid, quinic acid, citramalic acid, and isoquercitrin levels were higher in ‘Greenheart’ compared to ‘Daebo’ during cold storage. However, citric, isocitric, and threonic acids, gamma-aminobutyric acid, cysteine, β-alanine, titratable acidity, epicatechin, and proline were high in the ‘Daebo’ cultivar. Peel browning and pitting were positively correlated with soluble carbohydrates, organic acids, and amino acids but negatively correlated with individual phenolic compounds in ‘Daebo’ cultivar. The tricarboxylic acid cycle, glyoxylate and dicarboxylate metabolism, arginine and proline metabolism, biosynthesis of unsaturated fatty acids, arginine biosynthesis, and alanine, aspartate, and glutamate metabolism were upregulated in the ‘Daebo’ cultivar, whereas the phenylpropanoid pathway was upregulated in the ‘Greenheart’ cultivar. Our study showed that differentially upregulated pathways could lead to the contrasting development of necrotic peel disorders in these two hardy kiwifruit cultivars during cold storage. Our results suggest that the distinctive responses of metabolic analyses to chilling stress can contribute to susceptibility in chilling-induced necrotic peel disorders in cold-stored fruit of the ‘Daebo’ hardy kiwifruit cultivar.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"221 ","pages":"Article 113337"},"PeriodicalIF":6.4,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142723022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptome and metabolome analyses reveal the mechanism by which bagging treatment affects peel reddening in Orah mandarin","authors":"Ke Wen ,&nbsp;Xulin Li ,&nbsp;Tuo Yin ,&nbsp;Chaoying Chen ,&nbsp;Yinqiang Zi ,&nbsp;Ke Zhao ,&nbsp;Jinan Pu ,&nbsp;Wenxiu Yan ,&nbsp;Xuemei Wang ,&nbsp;Xianyan Zhou ,&nbsp;Xiaozhen Liu ,&nbsp;Hanyao Zhang","doi":"10.1016/j.postharvbio.2024.113336","DOIUrl":"10.1016/j.postharvbio.2024.113336","url":null,"abstract":"<div><div>Orah mandarin is a late-ripening citrus variety that is popular with consumers because of its red peel when ripe. However, the Orah mandarin from the Yunnan production area has difficulty reddening its peel. In this study, we applied different bagging treatments to Orah mandarin at the end of fruit expansion, and by analyzing the fruits for color difference values and intrinsic qualities, we found that the white bagging (W) treatment had the best color effect and contributed to the TSS, TA, SAR of the fruits. To further investigate the reasons for the changes in the skin color of Orah mandarin, we conducted a comprehensive analysis of W treatment and unbagging fruits via transcriptomic and metabolomic techniques. Forty-nine carotenoid metabolites, in which violaxanthin laurate, apocarotenal, and β-citraurin were the main substances responsible for the red color of Orah mandarin peels, were detected via UPLC-APCI-MS/MS targeted metabolomics analysis. Eighty-one structural genes related to carotenoid biosynthesis were screened via RNA-Seq, and the transcript levels of <em>LYCB2</em>, <em>LUT5–6</em>, <em>ZEP9</em>, <em>ZEP5</em>, and <em>NXS2</em> were positively correlated with the red carotenoid content. Correlation analysis revealed that the expression levels of <em>HSF2</em>, <em>MYB2</em>, and <em>WRKY2</em> were positively correlated with those of genes and metabolites (<em>R</em>^<em>2</em> &gt; 0.95). The qRT-PCR results also verified the expression of some of the genes and transcription factors. In addition, we identified a regulatory mechanism that promotes the red color trait in the peel of Orah mandarin, where bagging treatment increased the transcript levels of genes such as <em>LYCB2</em>, <em>LUT5–6</em>, <em>ZEP9</em>, <em>ZEP5</em>, and <em>NXS2</em>, which, in turn, increased the content of apocarotenoids and carotenoids in the peel, and <em>HSF2</em>, <em>MYB2</em>, <em>WRKY2</em>, and other transcription factors (TFs) interact with the above genes and metabolites, which further positively regulate carotenoid biosynthesis. The present study provides new insights into the effects of bagging on Orah mandarin fruits to offer valuable guidance for research on fruit color and carotenoid regulation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"221 ","pages":"Article 113336"},"PeriodicalIF":6.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual inhibition of vacuolar ATPase subunit H and iron availability induces intrinsic apoptosis in Penicillium digitatum from citrus fruit","authors":"Huilin Kang ,&nbsp;Yingying Zhao ,&nbsp;Gang Fan ,&nbsp;Shuzhen Yang ,&nbsp;Litao Peng ,&nbsp;Xinyue Lin","doi":"10.1016/j.postharvbio.2024.113327","DOIUrl":"10.1016/j.postharvbio.2024.113327","url":null,"abstract":"<div><div>The intracellular pH and iron homeostasis are essential for cell metabolic processes, which are important pathways to exploit novel antifungal targets. In this study, we evaluated the effects of simultaneously inhibition of vacuolar ATPase subunit H (VmaH) and iron availability on <em>Penicillium digitatum</em> and its action mode<em>.</em> Using RNA interference (RNAi) technology to silence the <em>VmaH</em> gene and treating the fungus with iron chelator ciclopirox coalmine (CPX), we found that the dual treatment inhibited the growth and pathogenicity of <em>P. digitatum</em>. Physiological and quantitative real-time PCR gene expression analysis indicated that this combination disrupted the pH and iron homeostasis within the fungal cells. Further results showed <em>VmaH</em> gene silencing combined with iron-chelation caused mitochondria dysfunction, endoplasmic reticulum (ER) stress, and an increase in reactive oxygen species (ROS) production. These effects led to the exacerbation of mitochondrial permeability transition pore (MPTP) opening, resulting in an increased release of calcium ion and cytochrome c from mitochondria. Consequently, there was an obvious regulation of apoptosis-related genes. Therefore, the combination of <em>VmaH</em> inhibition and iron chelation can induce intrinsic apoptosis in <em>P.digitatum</em> through ER stress-induced mitochondrial dysfunction, offering a novel strategy for the development of postharvest fungal pathogens control mechanisms.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"221 ","pages":"Article 113327"},"PeriodicalIF":6.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of pterostilbene inhibition of postharvest anthracnose on papaya fruit and antifungal mechanisms against Colletotrichum gloeosporioides","authors":"Jing Gao ,&nbsp;Shizi Zhang ,&nbsp;Yunfeng Xu ,&nbsp;Jichang Zhang ,&nbsp;Pengpeng Wu ,&nbsp;Lijuan Luo ,&nbsp;Lingyan Jiang","doi":"10.1016/j.postharvbio.2024.113304","DOIUrl":"10.1016/j.postharvbio.2024.113304","url":null,"abstract":"<div><div><em>Colletotrichum gloeosporioides</em> is the main pathogen causing anthracnose disease on a wide range of fruit and vegetables, leading to substantial economic losses. In present study, a plant natural product pterostilbene (PTE) was found effective in alleviation of postharvest anthracnose on papaya fruit. In addition, PTE inhibited the mycelium growth, conidium germination and appressorium formation of <em>C. gloeosporioides</em>. To investigate the underlying molecular mechanisms of antifungal activity of PTE, a transcriptomic analysis was performed. The transcriptomics identified 4131 and 3611 differentially expressed genes (DEGs) effected by PTE treatment at 4 h and 20 h, respectively. The gene function (GO) and metabolic pathway (KEGG) analysis of DEGs showed that PTE significantly affected the pathways related to carbohydrate and lipid metabolism, which play essential roles in maintaining the integrity and normal function of cell wall, plasma membrane and mitochondria of fungal cells. The biochemical assays as well as fluorescent and electron microscope observation demonstrated that the PTE treatment deformed the morphology and ultrastructure of mycelia and conidia, compromised the integrity of cell wall and plasma membrane, and impaired the function of cell wall, plasma membrane and mitochondria. Altogether, our findings improved the understanding of the antifungal activity of PTE and the underlying mechanisms of action at molecular level, thus supporting PTE as a potential agent in alleviating postharvest anthracnose by <em>C. gloeosporioides</em>.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"221 ","pages":"Article 113304"},"PeriodicalIF":6.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfur dioxide enhances the resistance of postharvest grape berries to gray mold through hydrogen peroxide signaling","authors":"Shijun Xing ,&nbsp;Quanming Tian ,&nbsp;Yige Zheng ,&nbsp;YuYao Yuan ,&nbsp;Zheng Zhang ,&nbsp;Yu Zhang ,&nbsp;Hao Zhang ,&nbsp;Jia Wei ,&nbsp;Bin Wu","doi":"10.1016/j.postharvbio.2024.113325","DOIUrl":"10.1016/j.postharvbio.2024.113325","url":null,"abstract":"<div><div>Gray mold, caused by <em>Botrytis cinerea</em>, presents a serious problem to the table grape industry. Sulfur dioxide (SO₂) can protect berries from pathogenic fungal infections by exerting biological functions, but the underlying mechanisms remain largely unknown. To investigate the mechanism by which SO₂ enhances postharvest grape disease resistance through reactive oxygen species (ROS) pathway, grapes were fumigated with SO₂ (500 μL L⁻¹) and then inoculated with <em>B. cinerea</em>. The results showed that SO₂ effectively inhibited the expansion of <em>B. cinerea</em> on postharvest grapes. SO₂ promoted the transient production of superoxide anions and hydrogen peroxide (H₂O₂) during the initial stage (in the first 9 h) by rapidly increasing the enzyme activities and gene expression of respiratory burst oxidase homologs and superoxide dismutase. From 1–7 d, the ROS and malondialdehyde levels were significantly reduced by SO₂. Further analysis showed that SO₂ can boost the antioxidant capacity of berries (total antioxidant capacity, and ABTS and DPPH scavenging capacities) by promoting ROS scavenging enzyme activities and the ascorbate-glutathione (AsA-GSH) cycle. In addition, the expression of ROS scavenging-related, AsA-GSH cycle-related genes, and <em>VvPR</em>s was effectively upregulated by SO₂. Together with correlation analysis results, we propose that the initial H₂O₂ signal contributes to improved antioxidant capacity and upregulation of <em>VvPR</em>s, which plays a crucial role in strengthening the long-term resistance of postharvest grapes to <em>B. cinerea</em>. In conclusion, SO₂ can enhance postharvest grape resistance to gray mold by leveraging the H₂O₂ signaling pathway, highlighting its pivotal role in activating the defense mechanisms of plants.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"221 ","pages":"Article 113325"},"PeriodicalIF":6.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mold in, mold out: Harvest bins harbor viable inoculum that can be reduced using novel sanitation methods to manage blue mold decay of apples","authors":"Dianiris Luciano-Rosario ,&nbsp;Johanny Castro ,&nbsp;Kari A. Peter ,&nbsp;Kerik D. Cox ,&nbsp;Jorge M. Fonseca ,&nbsp;Verneta L. Gaskins ,&nbsp;Wayne M. Jurick II","doi":"10.1016/j.postharvbio.2024.113323","DOIUrl":"10.1016/j.postharvbio.2024.113323","url":null,"abstract":"<div><div>Postharvest diseases account for major economic losses worldwide, while also contributing to food waste and loss. Blue mold, caused by <em>Penicillium expansum</em>, is one of the most prevalent postharvest diseases of pome fruit. This necrotrophic fungal pathogen is mycotoxigenic and presents a danger to food safety via the production of patulin. In addition, fungicide resistance threatens blue mold management strategies, creating a need for the development of novel control strategies. The apple storage/processing industry uses wood and plastic harvest bins to harvest and store apples for weeks, months, and even up to 1 year. Although it has been hypothesized that apple harvest bins can serve as a viable inoculum source to incite decay, there is no experimental evidence that has conclusively demonstrated this concept. In this study, we report the presence of <em>Penicillium</em> spp. in apple storage bins (plastic) in the mid-Atlantic USA and developed small scale methods to test 1) if harvest bins can serve as a source of inoculum for blue mold decay and 2) evaluate novel bin sanitation methods as preventative and curative applications. We demonstrated that apple harvest bin materials can serve as a source of inoculum when in direct contact with wounded apples. Additionally, it was shown that UV-C irradiation, sodium hypochlorite, and 2-phenylethanol effectively reduce inoculum viability on plastic materials in a curative fashion. It is envisioned that these findings can aid in the development of large-scale bin sanitation strategies and their applications have great potential to impact a broader range of postharvest pathogens and stored commodities.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"221 ","pages":"Article 113323"},"PeriodicalIF":6.4,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined physiological, biochemical, and multi-omic analyses provide insight into the cause of differential browning in early and late harvested fresh-cut yams","authors":"Xi Pang ,&nbsp;Zixin Lin ,&nbsp;Huafeng Liang ,&nbsp;Junyan Shi ,&nbsp;Shuzhi Yuan ,&nbsp;Xiaozhen Yue ,&nbsp;Xiaodi Xu ,&nbsp;Yaqi Zhao ,&nbsp;Zhifei Wei ,&nbsp;Jinhua Zuo ,&nbsp;Yuan Sui ,&nbsp;Bihong Feng ,&nbsp;Qing Wang","doi":"10.1016/j.postharvbio.2024.113324","DOIUrl":"10.1016/j.postharvbio.2024.113324","url":null,"abstract":"<div><div>Tissue browning reduces the cosmetic quality of fresh-cut yams and poses a marketing problem. The degree of browning of fresh-cut yams varies depending on when they are harvested. In this study, transcriptomic and metabolomic analyses were conducted in yams harvested in September (DHPS) and October (DHPO) to determine the mechanisms responsible for the browning of fresh-cut yams. Results showed that the browning index, total phenol and lignin content, polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia-lyase (PAL), 4-coumarate: CoA Ligase(4CL), and cinnamic acid-4-hydroxylase(C4H) activity in fresh-cut DHPO yams were lower than they were in fresh-cut DHPS yams. Flavonoids and catalase (CAT) activity remained consistently higher during storage in DHPO yams than in DHPS yams. The browning of fresh-cut DHPO yams was mitigated by the regulation of the expression of genes and the abundance of metabolites associated with phenolic metabolism, phytohormones, and signal transduction, as well as sucrose and starch metabolism. The level of browning enzyme activity in DHPO yams was significantly lower than it was in DHPS yams. Our study provides a basis for the selection of optimum harvest date for yams that will undergo fresh-cut processing.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"221 ","pages":"Article 113324"},"PeriodicalIF":6.4,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}