Postharvest Biology and Technology最新文献

Dielectric barrier discharge enhances ethylene decomposition and delays tomato fruit ripening during storage by cold atmospheric plasma 低温大气等离子体贮藏过程中，介质阻挡放电促进乙烯分解，延缓番茄果实成熟

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-30 DOI: 10.1016/j.postharvbio.2025.113688

Bingjie Ma , Yinan Zhang , Chongshan Zhong , Jiankang Cao

{"title":"Dielectric barrier discharge enhances ethylene decomposition and delays tomato fruit ripening during storage by cold atmospheric plasma","authors":"Bingjie Ma , Yinan Zhang , Chongshan Zhong , Jiankang Cao","doi":"10.1016/j.postharvbio.2025.113688","DOIUrl":"10.1016/j.postharvbio.2025.113688","url":null,"abstract":"<div><div>Although the application of cold atmospheric plasma (CAP) technology in the preservation of fruits has attracted much attention, the effect is limited because CAP generator is commonly designed to reduce microbial contaminations, instead of destroy ethylene. This work was aimed to develop a dielectric barrier discharge (DBD) technique to generate CAP, directly based on the evaluation of the effects of different discharge factors of DBD device on the decomposition of ethylene. The results showed that CAP (quantitatively indicated with ozone and nitrogen oxides) was rapidly generated and ethylene was effectively decomposed by the existing DBD reaction device, with discharge voltage 25 kV, input frequency 8 kHz, electrode gap 4 mm, dielectric thickness 2 mm, and equipped with a heat-resistant ceramic plate used as the dielectric material under normal atmospheric conditions. The decomposition rate of ethylene reached more than 90 % after 4 min of treatment with CAP generated by DBD, and completely 100 % after 6 min, whether high or low initial concentration of ethylene at the beginning of CAP generation. Moreover, the CAP treatments both high- and low- intensity generated by the DBD device effectively suppressed ethylene production, delayed the color change of green ripe tomatoes and the decrease in fruit hardness during storage. In conclusion, the optimization of the design of DBD device according to its capacity of decomposing ethylene provided an alternative approach to enhance effect of CAP on fruit preservation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"229 ","pages":"Article 113688"},"PeriodicalIF":6.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postharvest quality enhancement in high-russet ‘Conference’ pears: The role of organic farming and edible coatings 高赤褐色“会议”梨采后品质的提高：有机农业和食用涂层的作用

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-30 DOI: 10.1016/j.postharvbio.2025.113680

Marcos Guerra , Víctor Marcelo , Pedro Antonio Casquero

{"title":"Postharvest quality enhancement in high-russet ‘Conference’ pears: The role of organic farming and edible coatings","authors":"Marcos Guerra , Víctor Marcelo , Pedro Antonio Casquero","doi":"10.1016/j.postharvbio.2025.113680","DOIUrl":"10.1016/j.postharvbio.2025.113680","url":null,"abstract":"<div><div>Organic fruit production is gaining interest due to increasing consumer demand for healthier and more sustainable products. This is particularly relevant for high-quality cultivars with distinctive attributes that enhance their market recognition, such as ‘Conference’ pears. This study evaluated the effects of organic management practices on the quality and storability of ‘Conference’ pears grown in Northwestern Spain, known for their high sweetness, juiciness, and pronounced skin russeting. Additionally, the effectiveness of postharvest coatings in mitigating storage disorders was assessed. Organically grown pears exhibited improved instrumental and sensory quality, with higher color intensity, total soluble solids, and titratable acidity compared to conventionally grown pears. Moreover, organic management enhanced russeting, a key factor in consumer acceptance and premium positioning in the market. However, organically grown pears showed reduced storage potential due to increased susceptibility to shrivelling and decay. The application of Nutropit® combined with Bioxeda effectively reduced rot incidence. These findings highlight the potential of postharvest coatings as a strategy to improve the storage performance of organic pears. Further research should focus on optimizing natural coatings and preharvest strategies to enhance fruit quality and extend shelf life in organic supply chains.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"229 ","pages":"Article 113680"},"PeriodicalIF":6.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short-term postharvest UV-A and UV-B application enhances bioactive plant secondary metabolites in leafy vegetables 采后短期施用UV-A和UV-B可提高叶菜植物次生代谢产物的生物活性

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-30 DOI: 10.1016/j.postharvbio.2025.113679

Huihui Liu, Tobias Pöhnl, Zhengjie Ji, Susanne Neugart

{"title":"Short-term postharvest UV-A and UV-B application enhances bioactive plant secondary metabolites in leafy vegetables","authors":"Huihui Liu, Tobias Pöhnl, Zhengjie Ji, Susanne Neugart","doi":"10.1016/j.postharvbio.2025.113679","DOIUrl":"10.1016/j.postharvbio.2025.113679","url":null,"abstract":"<div><div>UV light is an important stimulator for bioactive secondary plant metabolites. Short-term postharvest applications are thought to enhance their concentration. Postharvest treatments with PAR light (control), UV-B (310 nm for 0.5 h, to 2 h), and two UV-A wavelengths (340 nm and 365 nm for 1.5 h, to 6 h) were applied to baby leaves of lettuce and chicory. Short-term UV-B (0.5 h and 1 h) and UV-A (1.5 h and 2 h) treatments achieved the highest accumulation of phenolic acids e.g. chicoric acid and sesquiterpene lactones e.g. 11(S),13-dihydro-8-deoxylactucin, in both lettuce and chicory. In chicory, chlorogenic acid levels increased with prolonged UV-A radiation. In addition, flavonoid glycosides were induced by both UV-B and UV-A light in chicory. These changes were accompanied by enhanced antioxidant activity after short-term postharvest UV treatments. UV-B (1 h) and UV-A (2 h) resulted in the highest levels of lutein and <em>β</em>-carotene in both lettuce and chicory. These findings underscore that short-term UV-B and UV-A treatments effectively modulate the concentration of bioactive compounds. Changes in sesquiterpene lactone concentrations may also have an impact on taste and consumer acceptance. Postharvest UV treatment can be applied as more cost effective and reliable than preharvest treatments, making them a real opportunity for commercial applications.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"229 ","pages":"Article 113679"},"PeriodicalIF":6.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous spermine delays the petal senescence of tree peony ‘Luoyanghong’ cut flowers by affecting physiological and molecular responses 外源精胺通过影响牡丹‘洛阳红’切花的生理和分子反应延缓花瓣衰老

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-29 DOI: 10.1016/j.postharvbio.2025.113684

Liuqing Qu , Di He , Dehu Su , Yanlong Zhang , Daoyang Sun , Jianrang Luo

{"title":"Exogenous spermine delays the petal senescence of tree peony ‘Luoyanghong’ cut flowers by affecting physiological and molecular responses","authors":"Liuqing Qu , Di He , Dehu Su , Yanlong Zhang , Daoyang Sun , Jianrang Luo","doi":"10.1016/j.postharvbio.2025.113684","DOIUrl":"10.1016/j.postharvbio.2025.113684","url":null,"abstract":"<div><div>Tree peony (<em>Paeonia suffruticosa</em>) is an important ornamental plant in China. However, it demonstrates limited postharvest longevity. To investigate the effect of exogenous spermine (Spm) on petal senescence of tree peony cut flowers, we treated ‘Luoyanghong’ cut flowers with 0.1 mmol L⁻¹ Spm. The results indicated that Spm extended the vase time and optimal viewing period of ‘Luoyanghong’ cut flowers by 33 h and 21 h, respectively; reduced malondialdehyde (MDA) content; increased the contents of osmotic regulatory substances and the activity of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX); reduced hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and superoxide anion levels; increased the content of antioxidant substances such as glutathione (GSH), ascorbate oxidase (AsA), total phenols, and total flavonoids; raised the contents of indole-3-acetic acid (IAA) and gibberellic acid (GA<sub>3</sub>) while decreasing the contents of 6-benzylaminopurine (6-BA), salicylic acid (SA), 1-aminocyclopropanecarboxylic acid (ACC), and abscisic acid (ABA); downregulated the expression of ethylene and abscisic acid biosynthesis and signal transduction genes <em>PsACS</em>, <em>PsEIN3</em>, <em>PsERF1</em>, <em>PsNCED1</em>, <em>PsABA2</em>, <em>PsPYR1</em>, <em>PsABF2</em> and senescence marker gene <em>PsSAG12</em> while upregulating the expression of <em>PsCTR1</em>. These results indicated that 0.1 mmol L⁻¹ Spm delayed the petal senescence of tree peony ‘Luoyanghong’ cut flowers and prolonged the postharvest vase life.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113684"},"PeriodicalIF":6.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water transport, vascular bundle integrity, and enzymatic activity in rambutan pericarp and spine browning 红毛丹果皮和棘褐变过程中的水运输、维管束完整性和酶活性

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-29 DOI: 10.1016/j.postharvbio.2025.113685

Hanchi Guan , Shoukui Tao , Yonggui Pan , Weimin Zhang , Zhanhong Han , Hanyue Yang , Yaxin Tan

{"title":"Water transport, vascular bundle integrity, and enzymatic activity in rambutan pericarp and spine browning","authors":"Hanchi Guan , Shoukui Tao , Yonggui Pan , Weimin Zhang , Zhanhong Han , Hanyue Yang , Yaxin Tan","doi":"10.1016/j.postharvbio.2025.113685","DOIUrl":"10.1016/j.postharvbio.2025.113685","url":null,"abstract":"<div><div>Postharvest rambutan (<em>Nephelium lappaceum</em> L.) rapidly loses its visual quality owing to accelerated water loss and browning of the spines and pericarp. Notably, the water loss and browning of the rambutan spines progress significantly quicker than that of the pericarp. To investigate the impact of the loss of water on the structural changes of the cell induced by enzymatic browning, the water status and distribution of rambutan peel during storage were analyzed. In addition, the ultrastructure of the pericarp and spines was observed. The spines lost significantly more water and browned more intensively compared to the pericarp. The low field NMR images suggested that the supply of water from the pericarp to the spines ceased during storage. Ultrastructural observations showed that the cell structure of vessels in the xylem was destroyed, and the phloem disappeared in the spines during storage. This probably terminated the hydraulic connectivity between the pericarp and spines. The severe water loss in the spines destroyed the plasma and vesicle membranes and the mitochondrial structure. Compared to the pericarp, the spines had higher levels of activities of polyphenol oxidase, peroxidase, and phenylalanine ammonia lyase during storage, which was accompanied by a more rapid decrease in the phenolics and a quicker accumulation of o-quinones. These results suggested that severe water loss in the spines may be associated with disruption of the vascular bundles, which leads to an insufficient water supply from the pericarp. The severe water loss further caused a breakdown of cellular compartmentalization, which facilitated contact between the enzymatic related to browning and the phenolics. Thus, this exacerbated browning in the spines.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113685"},"PeriodicalIF":6.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical profile and quality variations during storage of wild leafy vegetables: The antagonistic role of copper and ascorbic acid 野生叶菜贮藏期间的生化特征和品质变化：铜和抗坏血酸的拮抗作用

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-29 DOI: 10.1016/j.postharvbio.2025.113686

Stefania Stelluti , Simone Ravetto Enri , Nicole Mélanie Falla , Sonia Demasi , Giampiero Lombardi , Valentina Scariot

{"title":"Biochemical profile and quality variations during storage of wild leafy vegetables: The antagonistic role of copper and ascorbic acid","authors":"Stefania Stelluti , Simone Ravetto Enri , Nicole Mélanie Falla , Sonia Demasi , Giampiero Lombardi , Valentina Scariot","doi":"10.1016/j.postharvbio.2025.113686","DOIUrl":"10.1016/j.postharvbio.2025.113686","url":null,"abstract":"<div><div>Wild leafy vegetables (WLVs) are valuable components of traditional diets due to their rich phytochemical content and potential health benefits. However, their perishability after harvest limits their use and commercial potential. This study investigated the postharvest quality dynamics of eight WLV species, focusing on biochemical changes including pigment content, total phenolic content (TPC), and antioxidant activity. Additionally, the role of phenolic compounds, copper, and ascorbic acid in tissue browning was explored. The results highlighted significant differences among species in their postharvest behaviour. The most pronounced biochemical changes occurred ten days after harvest. <em>Alchemilla xanthochlora</em> consistently maintained high antioxidant quality, while <em>Bistorta officinalis</em> preserved better overall quality. Conversely, <em>Phyteuma betonicifolium</em> and <em>Blitum bonus-henricus</em> exhibited progressive deterioration. Some species, such as <em>Achillea millefolium</em> and <em>Silene vulgaris</em>, maintained stable but moderate quality, whereas <em>Taraxacum officinale</em> and <em>Plantago lanceolata</em> showed initial suboptimal quality that worsened postharvest. This study confirmed that copper and ascorbic acid play antagonist roles in preserving antioxidants of WLVs: higher copper levels at harvest enhance postharvest <em>o</em>-quinone formation, whereas ascorbic acid exerts an opposing influence. Most of the studied species demonstrated the ability to retain quality for extended storage periods, suggesting opportunities for their broader inclusion in sustainable food systems.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113686"},"PeriodicalIF":6.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MaWLIM2-MaZFP6 transcriptional regulatory module directly represses E3 ligase gene MaRZF1 to regulate high temperature-suppressed chlorophyll catabolism in banana fruit MaWLIM2-MaZFP6转录调控模块直接抑制E3连接酶基因MaRZF1，调控香蕉果实高温抑制叶绿素分解代谢

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-29 DOI: 10.1016/j.postharvbio.2025.113674

Qi Luo, Wei Wei, Jian-fei Kuang, Jian-ye Chen, Wang-jin Lu, Wei Shan

{"title":"MaWLIM2-MaZFP6 transcriptional regulatory module directly represses E3 ligase gene MaRZF1 to regulate high temperature-suppressed chlorophyll catabolism in banana fruit","authors":"Qi Luo, Wei Wei, Jian-fei Kuang, Jian-ye Chen, Wang-jin Lu, Wei Shan","doi":"10.1016/j.postharvbio.2025.113674","DOIUrl":"10.1016/j.postharvbio.2025.113674","url":null,"abstract":"<div><div>Banana (<em>Musa acuminata</em>, AAA group) fruit ripening at high temperatures (HTs) exceeding 24 °C prohibits the yellow peel development and leads to green ripening, which dramatically diminishes its marketability. Our recent study found that HT induced MaRZF1 (RING Zinc Finger 1)-mediated degradation of MaSGR1 (STAY-GREEN 1) to inhibit chlorophyll breakdown, but how HT activates <em>MaRZF1</em>'s expression is completely unclear. The present study identifies two transcription factors (TFs), MaWILIM2 (widely expressed LIM) and MaZFP6 (zinc finger protein), as potential binding proteins for the <em>MaRZF1</em> promoter. Both <em>MaWILIM2</em> and <em>MaZFP6</em> expression levels were lower in 30 °C green-ripened fruit than in 20 °C yellow-ripened fruit. In banana fruit, overexpressing <em>MaWLIM2</em> and <em>MaZFP6</em> suppressed <em>MaRZF1</em> expression and mitigated chlorophyll catabolism inhibition caused by HTs. More importantly, MaWLIM2 interacted with MaZFP6 to synergistically repress the <em>MaRZF1</em> promoter activity. Our findings demonstrate that MaWLIM2 and MaZFP6 form a dynamic transcriptional regulatory module that represses <em>MaRZF1</em> and controls HTs-inhibited chlorophyll degradation in banana fruit. These findings enhance our understanding of transcriptional regulation in fruit quality decline due to temperature stress and are expected to aid in developing strategies to manage banana green-ripening.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113674"},"PeriodicalIF":6.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen-rich water delays post-harvest yellowing in broccoli by inhibiting ethylene and ABA levels, thereby reducing chlorophyll degradation and carotenoid accumulation 富氢水通过抑制乙烯和ABA水平，从而减少叶绿素降解和类胡萝卜素积累，延缓西兰花收获后的黄变

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-27 DOI: 10.1016/j.postharvbio.2025.113661

Yi Wang , Xintong Chen , Wei Chen , Zhenfeng Yang , Liyu Shi , Xuewen Li , Shifeng Cao , Wei Song

{"title":"Hydrogen-rich water delays post-harvest yellowing in broccoli by inhibiting ethylene and ABA levels, thereby reducing chlorophyll degradation and carotenoid accumulation","authors":"Yi Wang , Xintong Chen , Wei Chen , Zhenfeng Yang , Liyu Shi , Xuewen Li , Shifeng Cao , Wei Song","doi":"10.1016/j.postharvbio.2025.113661","DOIUrl":"10.1016/j.postharvbio.2025.113661","url":null,"abstract":"<div><div>To investigate the mechanism of yellowing and explore control methods, this study focused on the impact and mechanism of hydrogen-rich water (HRW) treatment on broccoli floret yellowing. The research results indicate that HRW treatment maintains the structural integrity of chloroplasts in broccoli florets during post-harvest storage, slowing down the degradation of chlorophyll and the accumulation of carotenoids. RT-qPCR results show that HRW treatment inhibits the expression of chlorophyll degradation genes <em>BoNOL1/6/10</em>, <em>BoPAO</em>, <em>BoPOX</em>, <em>BoSGR1</em>, <em>BoCLH1</em>, <em>BoNCY</em>, and <em>BoNYC</em>, as well as carotenoid-related biosynthesis genes <em>BoPSY</em>, <em>BoPDS</em>, <em>BoZDS</em>, <em>BoZISO</em>, <em>BoCRTISO</em>, <em>BoCYP97C1</em>, and <em>BoCCS-1</em>, while increasing the expression level of <em>BoCCD1/4</em>. Furthermore, HRW treatment inhibits the production of endogenous ethylene through downregulation of its biosynthetic genes <em>BoACO1/2</em> and <em>BoACS1/2/7</em>. Simultaneously, HRW treatment downregulates ABA biosynthesis genes <em>BoNCED2/3</em> and <em>BoABA2</em>, resulting in a significant reduction in ABA levels. In summary, the HRW treatment delays the yellowing of broccoli after harvest by inhibiting ethylene and ABA levels, thereby reducing the degradation of chlorophyll and the accumulation of carotenoids.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113661"},"PeriodicalIF":6.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factor CsWRKY76 potentially involved in accumulation of scoparone and pentamethoxyflavone in citrus fruit stimulated by salicylic acid 转录因子CsWRKY76可能参与水杨酸刺激柑橘果实中天冬酮和五甲基甲黄酮的积累

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-27 DOI: 10.1016/j.postharvbio.2025.113672

Shuqing Xu, Shuzhen Yang, Yanting Yang, Meihong Zhang, Anqi Tang, Yi Li, Litao Peng

{"title":"Transcription factor CsWRKY76 potentially involved in accumulation of scoparone and pentamethoxyflavone in citrus fruit stimulated by salicylic acid","authors":"Shuqing Xu, Shuzhen Yang, Yanting Yang, Meihong Zhang, Anqi Tang, Yi Li, Litao Peng","doi":"10.1016/j.postharvbio.2025.113672","DOIUrl":"10.1016/j.postharvbio.2025.113672","url":null,"abstract":"<div><div>Salicylic acid (SA) is a potent elicitor of phytoalexins in citrus fruit, yet the transcriptional regulatory mechanisms underlying SA-induced disease resistance remain unclear. Here, we identified <em>CsWRKY76</em> as a key transcription factor (TF) positively regulating SA-mediated accumulation of scoparone and 3, 5, 7, 3′, 4′-pentamethoxyflavone (PMF) in citrus (<em>Citrus sinensis</em>) peels inoculated with <em>Penicillium italicum</em>. <em>CsWRKY76</em> expression was strongly correlated with phytoalexin levels and significantly upregulated by SA. Subcellular localization confirmed its nuclear targeting, while yeast one-hybrid (Y1H) assay revealed transcriptional activation and specific binding to the W-box motif. Transient overexpression of <em>CsWRKY76</em> in citrus peels elevated scoparone (74.8 %) and PMF (24.3 %) levels, concomitant with upregulation of phenylpropanoid pathway genes (<em>Cs4CL</em>, <em>CsPAL</em>, <em>CsF6'H1</em>, <em>CsCOMT</em>). Conversely, silencing <em>CsWRKY76</em> reduced scoparone and PMF accumulation by 49.7 % and 57.8 % with downregulation of the involved genes. Notably, <em>CsWRKY76</em> indirectly regulated these biosynthetic genes, suggesting a complex regulatory network. Our findings elucidate <em>CsWRKY76</em> as a critical regulator of SA-induced defense responses in citrus, providing a molecular target for enhancing postharvest disease resistance.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113672"},"PeriodicalIF":6.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DCC and ADP regulate the pulp breakdown in Phomopsis longanae Chi-inoculated longan via modulating energy levels and the metabolisms of reactive oxygen species and membrane lipids DCC和ADP通过调节能量水平、活性氧和膜脂代谢来调节龙眼接种后的果肉分解

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-26 DOI: 10.1016/j.postharvbio.2025.113662

Jin Lin , Yunman Chen , Meiqi Huang , Huili Zhang , Huafeng Hong , Mengshi Lin , Ruiling Zhuo , Yihui Chen , Hetong Lin , Yifen Lin

{"title":"DCC and ADP regulate the pulp breakdown in Phomopsis longanae Chi-inoculated longan via modulating energy levels and the metabolisms of reactive oxygen species and membrane lipids","authors":"Jin Lin , Yunman Chen , Meiqi Huang , Huili Zhang , Huafeng Hong , Mengshi Lin , Ruiling Zhuo , Yihui Chen , Hetong Lin , Yifen Lin","doi":"10.1016/j.postharvbio.2025.113662","DOIUrl":"10.1016/j.postharvbio.2025.113662","url":null,"abstract":"<div><div>Compared to <em>P. longanae</em>-inoculated longan, DCC + <em>P. longanae</em>-inoculated longan exhibited an elevated pulp breakdown index and higher cell membrane permeability, along with the reduced ATP levels and energy charge. Additionally, DCC + <em>P. longanae</em>-inoculated longan exhibited higher activities of membrane lipid-degrading enzymes (MLDEs), including PI-PLC, PC-PLC, PLD, DGK, PAP, lipase, and LOX, as well as higher levels of DAG, PA and SFAs. Conversely, the contents of PI, PC and USFAs were reduced, accompanied by lower U/S and IUFA. Besides, DCC + <em>P. longanae</em>-inoculated longan displayed lower activities of SOD, CAT, APX, GPX, MDHAR, DHAR, and GR, along with lower reducing power and DPPH radical scavenging ability, lower levels of AsA and GSH, and higher levels of DHA, GSSG, H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>·−</sup> production rate, and MDA. These outcomes indicate that DCC treatment expedited pulp breakdown in <em>P. longanae</em>-inoculated longan by exacerbating energy deficiency, which activated MLDEs activities and damaged cell membrane structure. Furthermore, the insufficient energy supply also impaired ROS-scavenging capacity, thereby leading to ROS accumulation, membrane lipid peroxidation, and loss of cell membrane integrity. In contrast, ADP + <em>P. longanae</em>-inoculated longans exhibited the opposite effects. The results of present work can provide new insight into the mechanism of longan pulp breakdown, and offer new strategies such as the application of ADP for suppressing longan pulp breakdown to extend the shelf life of fresh longan.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113662"},"PeriodicalIF":6.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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