Postharvest Biology and Technology最新文献

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Ethylene-induced postharvest ‘Guifei’ mango peel coloration: Activation of the MiPAL1 promoter by MiERF5 and the MiERF5-MiMYB7 complex 乙烯诱导的收获后“贵非”芒果皮着色:MiERF5和MiERF5- mimyb7复合物激活MiPAL1启动子
IF 6.8 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-10-18 DOI: 10.1016/j.postharvbio.2025.113999
Xin Wang , Yaqi Dou , Yu Wang , Pan Guo , Yuanhui Gao , Jinhe Li , Yuanzhi Shao , Wen Li
{"title":"Ethylene-induced postharvest ‘Guifei’ mango peel coloration: Activation of the MiPAL1 promoter by MiERF5 and the MiERF5-MiMYB7 complex","authors":"Xin Wang ,&nbsp;Yaqi Dou ,&nbsp;Yu Wang ,&nbsp;Pan Guo ,&nbsp;Yuanhui Gao ,&nbsp;Jinhe Li ,&nbsp;Yuanzhi Shao ,&nbsp;Wen Li","doi":"10.1016/j.postharvbio.2025.113999","DOIUrl":"10.1016/j.postharvbio.2025.113999","url":null,"abstract":"<div><div>Peel coloration strongly influences their sensory quality and commercial value. While ethylene (ETH) promotes and 1-methylcyclopropene (1-MCP) inhibits mango peel coloration, the underlying molecular mechanisms remain incompletely understood. This study investigated the effects of ETH and 1-MCP on ‘Guifei’ mango, focusing on the regulation of the <em>MiPAL1</em> promoter by MiERF5 and MiMYB7. ETH enhanced the activities of anthocyanin biosynthesis enzymes (PAL, CHI, ANS, DFR, UFGT) and anthocyanin accumulation, whereas 1-MCP suppressed these processes. ETH upregulated <em>MiPAL1</em> and <em>MiERF5</em>, while 1-MCP increased <em>MiMYB7</em> expression. Nuclear-localized MiERF5 directly bound and activated the <em>MiPAL1</em> promoter (verified by Y1H and DLR assays), whereas MiMYB7 repressed it. Crucially, Y2H, BiFC, and LCI assays revealed that a MiERF5-MiMYB7 protein complex binds to <em>MiPAL1</em>, alleviating MiMYB7-mediated repression. Functional validation in mango discs demonstrated that <em>MiMYB7</em> overexpression inhibited coloration by downregulating <em>MiPAL1</em>, whereas <em>MiERF5</em> overexpression or co-expression of <em>MiERF5</em> + <em>MiMYB7</em> activated <em>MiPAL1</em> and anthocyanin synthesis. These findings demonstrate that MiERF5 and the MiERF5-MiMYB7 complex positively regulate anthocyanin biosynthesis, revealing a novel regulatory switch for ethylene-mediated peel coloration and potential targets for improving postharvest fruit quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113999"},"PeriodicalIF":6.8,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcription factor PpMYB73 inhibits PpPPO1 transcription and flesh browning in hydrogen sulfide-mitigated chilling injury of peach fruit 转录因子PpMYB73抑制pppppo1转录和硫化氢减轻桃果冷害的果肉褐变
IF 6.8 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-10-18 DOI: 10.1016/j.postharvbio.2025.114004
Yaqin Zhao , Ziao Liu , Liangyi Zhao , Yueyang Xi , Zhengguo Wu , Yonghua Zheng , Peng Jin
{"title":"Transcription factor PpMYB73 inhibits PpPPO1 transcription and flesh browning in hydrogen sulfide-mitigated chilling injury of peach fruit","authors":"Yaqin Zhao ,&nbsp;Ziao Liu ,&nbsp;Liangyi Zhao ,&nbsp;Yueyang Xi ,&nbsp;Zhengguo Wu ,&nbsp;Yonghua Zheng ,&nbsp;Peng Jin","doi":"10.1016/j.postharvbio.2025.114004","DOIUrl":"10.1016/j.postharvbio.2025.114004","url":null,"abstract":"<div><div>Flesh browning, a major chilling injury (CI) symptom in peach fruit, was alleviated by hydrogen sulfide (H<sub>2</sub>S) treatment, although its molecular mechanism remained unclear. Here, we identified five <em>peach polyphenol oxidase</em> genes (<em>PpPPO</em>), with <em>PpPPO1</em> exhibiting the highest expression but inhibited by H<sub>2</sub>S application during CI development. Chlorogenic acid (CGA), the dominant phenolic in peach fruit, was reduced in fruit transiently overexpressing <em>PpPPO1</em>. Molecular docking confirmed that PpPPO1 binds CGA via hydrogen bonds and hydrophobic interactions. PpPPO1 may act as the main contributor to enzymatic browning in peach fruit. H₂S treatment upregulated <em>PpMYB73</em> expression, an R2R3-MYB transcription repressor, and enhanced its suppression of <em>PpPPO1</em> transcription, thereby negatively regulating phenolic oxidation. Transient overexpression of <em>PpMYB73</em> caused a reduction in <em>PpPPO1</em> expression and PPO activity, increasing CGA accumulation and thereby suppressing soluble quinones production and flesh browning in peach fruit. While transiently silencing <em>PpMYB73</em> increased soluble quinones content and exacerbated flesh browning, further confirming the inhibitory role of PpMYB73 in flesh browning. Collectively, our findings established that PpMYB73 acted as a transcriptional repressor of <em>PpPPO1</em> expression, implicating H<sub>2</sub>S-alleviated CI in peach fruit, and uncovered a novel regulatory network controlling CI.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 114004"},"PeriodicalIF":6.8,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antifungal activity of non-thermal atmospheric pressure plasma against postharvest mango anthracnose, Colletotrichum siamense 非热常压等离子体对芒果采后炭疽病的抑菌活性研究
IF 6.8 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-10-17 DOI: 10.1016/j.postharvbio.2025.113994
Thummanoon Boonmee , Terd Disayathanoowat , Laedlugkana Wongthaveethong , Suphichaya Nakpla , Chainarong Sinpoo , Kamonporn Panngom , Veeranan Chaimanee
{"title":"Antifungal activity of non-thermal atmospheric pressure plasma against postharvest mango anthracnose, Colletotrichum siamense","authors":"Thummanoon Boonmee ,&nbsp;Terd Disayathanoowat ,&nbsp;Laedlugkana Wongthaveethong ,&nbsp;Suphichaya Nakpla ,&nbsp;Chainarong Sinpoo ,&nbsp;Kamonporn Panngom ,&nbsp;Veeranan Chaimanee","doi":"10.1016/j.postharvbio.2025.113994","DOIUrl":"10.1016/j.postharvbio.2025.113994","url":null,"abstract":"<div><div>Anthracnose, caused by <em>Colletotrichum siamense</em>, is a major postharvest disease of mango (<em>Mangifera indica</em> L.), contributing to significant economic losses during storage and distribution. This study investigated the antifungal efficacy of non-thermal atmospheric pressure plasma (NTP) and plasma-activated water (PAW), generated using argon and helium gases, for the inactivation of <em>C. siamense</em> and the suppression of anthracnose symptoms in mango fruits. Complete inhibition of mycelial growth was observed following plasma exposure for up to seven days. Direct NTP treatments significantly reduced spore viability, with helium plasma achieving 18.53–49.00 % inactivation and argon plasma achieving 13.15–47.21 % based on spore counts. The highest reduction in viable colony counts (up to 97.34 %) was observed following 10 min of argon plasma exposure at a flow rate of 0.25 Lpm. PAW treatments also significantly reduced spore viability, with argon-based PAW showing greater antifungal activity (35.56–53.77 %) than helium-based PAW (19.44–41.39 %). At a flow rate of 0.25 Lpm and an exposure time of 10 min, PAW generated using argon and helium resulted in reductions in viable colony counts of 64.77 % and 72.35 %, respectively. Membrane integrity assays revealed increased leakage of UV-absorbing intracellular components, while fluorescence-based viability staining confirmed reduced spore viability following both treatments. Scanning electron microscopy further revealed spore surface deformation consistent with oxidative damage. In vivo assays on detached Nam Dok Mai and Chok Anan mangoes demonstrated significant suppression of anthracnose lesion development from both mycelia and spore inoculations, particularly during the later stages of incubation. These results indicate that NTP and PAW are promising chemical-free approaches for managing postharvest anthracnose in mango. Further studies are warranted to refine treatment conditions and to evaluate their compatibility with existing postharvest practices and their effects on fruit quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113994"},"PeriodicalIF":6.8,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
From firm to soft: Maturity-driven mechanical damage behavior of apples 从硬到软:苹果成熟驱动的机械损伤行为
IF 6.8 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-10-17 DOI: 10.1016/j.postharvbio.2025.114003
Zixu Chen , Linlong Jing , Ruofei Liu , Huawei Yang , Linlin Sun , Xinpeng Cao , Yongxian Wang , Shenghui Fu , Hongjian Zhang , Jinxing Wang
{"title":"From firm to soft: Maturity-driven mechanical damage behavior of apples","authors":"Zixu Chen ,&nbsp;Linlong Jing ,&nbsp;Ruofei Liu ,&nbsp;Huawei Yang ,&nbsp;Linlin Sun ,&nbsp;Xinpeng Cao ,&nbsp;Yongxian Wang ,&nbsp;Shenghui Fu ,&nbsp;Hongjian Zhang ,&nbsp;Jinxing Wang","doi":"10.1016/j.postharvbio.2025.114003","DOIUrl":"10.1016/j.postharvbio.2025.114003","url":null,"abstract":"<div><div>Understanding the mechanical damage behavior of apples at different maturity stages is crucial for reducing postharvest losses and maintaining quality during transportation. In this study, a combined approach of compression experiment and finite element simulation was employed to investigate the damage characteristics of Fuji apples under compressive loading across four maturity levels. Controlled compression experiments were conducted using a universal testing machine to quantify the force-deformation response and determine critical damage thresholds. The experimentally derived mechanical parameters were used to calibrate a finite element model developed through reverse engineering. This model effectively simulated the internal stress distribution and deformation behavior of apples during compression. Results indicated a linear positive correlation between storage time and damage volume. Less mature apples exhibited higher fracture resistance and lower stress localization, while more mature apples, with reduced stiffness, were more prone to internal damage and higher stress concentration. The deviation between simulated and measured damage volumes ranged from 3.25 % to 16.8 %, confirming the validity of the developed model in replicating realistic compression scenarios. These findings provide valuable insights for designing harvesting, grading, and packaging equipment tailored to apples at varying maturity stages, ultimately enhancing product quality and reducing losses across the supply chain.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 114003"},"PeriodicalIF":6.8,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
S.cerevisiae NX2320 primes JA/ET signaling and phenylpropanoid metabolism to enhance postharvest resistance of grape fruit against Botrytis cinerea: An integrated transcriptome-metabolome study s.s erevisiae NX2320启动JA/ET信号和苯丙素代谢,增强葡萄果实对葡萄灰霉病的抗性:一项综合转录组-代谢组研究
IF 6.8 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-10-16 DOI: 10.1016/j.postharvbio.2025.113997
Xiaohua Ma , Jie Guo , Siheng Shen , Deming Liu , Yanlin Liu , Xiaoming Chen , Xixi Zhao
{"title":"S.cerevisiae NX2320 primes JA/ET signaling and phenylpropanoid metabolism to enhance postharvest resistance of grape fruit against Botrytis cinerea: An integrated transcriptome-metabolome study","authors":"Xiaohua Ma ,&nbsp;Jie Guo ,&nbsp;Siheng Shen ,&nbsp;Deming Liu ,&nbsp;Yanlin Liu ,&nbsp;Xiaoming Chen ,&nbsp;Xixi Zhao","doi":"10.1016/j.postharvbio.2025.113997","DOIUrl":"10.1016/j.postharvbio.2025.113997","url":null,"abstract":"<div><div>Gray mold caused by <em>Botrytis cinerea</em> is one of the major postharvest diseases of fruits during storage and transportation, and traditional chemical control faces challenges such as pesticide residues and the development of pathogen resistance. As a safe antagonistic yeast, <em>S. cerevisiae</em> NX2320 shows promising potential for biological control. In this study, various fruits such as apple, apricot, yellow peach, Shine-Muscat grape, Red Globe grape, and tomato were used as a model to systematically evaluate the inhibitory effect of <em>S. cerevisiae</em> NX2320 on gray mold and its impact on fruit quality, the molecular mechanism of its induced resistance in Red Globe grape was elucidated through integrated transcriptomic and metabolomic analyses. Results showed that yeast treatment reduced fruit decay incidence after 6 days of storage and significantly maintained higher vitamin C levels, titratable acidity, and firmness. <em>S. cerevisiae</em> NX2320 rapidly colonized the fruit surface and markedly enhanced the activities of antioxidant-related parameters such as peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), while reducing malondialdehyde (MDA) levels. Transcriptome analysis identified 2473 differentially expressed genes, with transcription factors (TFs) including <em>WRKY</em>, <em>ERF</em>, and <em>MYB</em> significantly upregulated and enriched in plant hormone signaling and phenylpropanoid biosynthesis pathways. Metabolomic analysis revealed 138 differential metabolites, including defense-related phenolic and flavonoid compounds such as resveratrol and polydatin. Integrated multi-omics analysis demonstrated that <em>S. cerevisiae</em> NX2320 activates a systemic resistance network by coordinating ROS signaling, hormonal regulation, and secondary metabolic reprogramming. This study provides theoretical insights and potential microbial resources for the green postharvest management of grape diseases.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113997"},"PeriodicalIF":6.8,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome-wide characterization of the CsGPAT gene family and functional insights into citrus fruit wound healing CsGPAT基因家族的全基因组特征及其在柑橘类水果伤口愈合中的功能研究
IF 6.8 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-10-15 DOI: 10.1016/j.postharvbio.2025.113969
Siyu Li , Xiaoquan Gao , Jialin Chen , Honghai Li , Lanhua Yi , Jian Ming , Kaifang Zeng
{"title":"Genome-wide characterization of the CsGPAT gene family and functional insights into citrus fruit wound healing","authors":"Siyu Li ,&nbsp;Xiaoquan Gao ,&nbsp;Jialin Chen ,&nbsp;Honghai Li ,&nbsp;Lanhua Yi ,&nbsp;Jian Ming ,&nbsp;Kaifang Zeng","doi":"10.1016/j.postharvbio.2025.113969","DOIUrl":"10.1016/j.postharvbio.2025.113969","url":null,"abstract":"<div><div>Postharvest citrus fruit are susceptible to mechanical damage, and wound healing via suberin deposition provides a critical physical barrier against pathogen invasion. Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the first step in suberin biosynthesis by acylating glycerol-3-phosphate to lysophosphatidic acid, but <em>GPAT</em> genes' roles in citrus wound healing remain unclear. In this study, eight <em>CsGPATs</em> were identified in citrus, in which the functions of <em>CsGPAT2</em>, <em>CsGPAT3</em> and <em>CsGPAT4</em> were mainly analyzed. Changes in suberin content in citrus wounds after transient overexpression and virus-induced gene silencing (VIGS) of the <em>CsGPATs</em> were observed by orthogonal fluorescence microscopy. Meanwhile, the growth of green mold on citrus fruit was observed, and GPAT enzyme activity was measured. Overexpression of the <em>CsGPATs</em> significantly improved wound healing, decreased green mold incidence and lesion diameter, and significantly increased GPAT enzyme activity. The GC-MS analysis indicated that the transient overexpression of the <em>CsGPATs</em> promoted the accumulation of suberin monomers. In addition, the expression levels of key genes involved in the suberin synthesis pathway were notably elevated. In conclusion, <em>CsGPATs</em> facilitated suberin accumulation, which enhanced wound healing and effectively suppressed green mold development in citrus fruit. This research offers novel perspectives on the regulatory mechanisms underlying wound healing, which are pivotal for enhancing the postharvest quality of citrus fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113969"},"PeriodicalIF":6.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mixed packaging-induced fresh-cut carrot and broccoli flavor change is associated with respiratory and energy metabolism 混合包装引起的鲜切胡萝卜和西兰花风味变化与呼吸和能量代谢有关
IF 6.8 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-10-14 DOI: 10.1016/j.postharvbio.2025.113996
Yuqi Bin , Mingyu Wang , Ke Wu , Ruonan Guo , Ke Feng , Aili Jiang , Chen Chen
{"title":"Mixed packaging-induced fresh-cut carrot and broccoli flavor change is associated with respiratory and energy metabolism","authors":"Yuqi Bin ,&nbsp;Mingyu Wang ,&nbsp;Ke Wu ,&nbsp;Ruonan Guo ,&nbsp;Ke Feng ,&nbsp;Aili Jiang ,&nbsp;Chen Chen","doi":"10.1016/j.postharvbio.2025.113996","DOIUrl":"10.1016/j.postharvbio.2025.113996","url":null,"abstract":"<div><div>Fresh-cut carrot and broccoli are often packaged as mixes in the market, but their impact on flavor quality is unclear. Herein, we measured the flavor changes in mixed packaging of fresh-cut carrot and broccoli during storage at 15 ℃. Results showed that mixed packaging accelerated the loss of characteristic flavor compounds, while inhibited the accumulation of off-flavor substances of fresh-cut carrot and broccoli. The respiration rate, fructose-6-phosphate (6-P-F) and pyruvic acid contents, pyruvate kinase (PK) and phosphofructokinase (PFK) gene expression and activities of fresh-cut broccoli were elevated after mixed packaging. For fresh-cut carrot, mixed packaging also increased the respiration rate, pyruvic acid content and the expression of <em>PK</em>, while reduced 6-P-F content, PK and PFK activities, and <em>PFK</em> gene expression. Furthermore, mixed packaging improved energy level, cytochrome C oxidase activity, and up-regulated the gene expression of most <em>ATP synthase</em> of both fresh-cut carrot and broccoli. We therefore propose that the flavor changes in mixed packageing of fresh-cut carrot and broccoli might be related to respiration and energy metabolism.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113996"},"PeriodicalIF":6.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A nonlinear classification method for online sorting of navel orange SSC based on near-infrared spectroscopy and data augmentation 基于近红外光谱和数据增强的脐橙SSC非线性在线分类方法
IF 6.8 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-10-14 DOI: 10.1016/j.postharvbio.2025.113990
Shaohui Yu , Jing Liu
{"title":"A nonlinear classification method for online sorting of navel orange SSC based on near-infrared spectroscopy and data augmentation","authors":"Shaohui Yu ,&nbsp;Jing Liu","doi":"10.1016/j.postharvbio.2025.113990","DOIUrl":"10.1016/j.postharvbio.2025.113990","url":null,"abstract":"<div><div>As a non-invasive detection method, near-infrared spectroscopy (NIR) has demonstrated significant potential for application in assessing fruit quality and sorting. However, during the online fruit sorting process, multiple factors affect the sorting accuracy. To address the challenges of limited sample size, heterogeneous quality, and the intricate nonlinear relationship between detection indices and spectral data in online sorting, this paper presents a data augmentation approach for the online sorting of navel oranges based on the soluble solids content (SSC), which integrates error rate and probability mass weighting. Firstly, cluster analysis was performed on the SSC, and the R<sup>2</sup> statistic and the elbow rule were introduced to determine the optimal number of clusters. The training set and test set were divided using the Monte Carlo random sampling method. Subsequently, the training set was augmented by incorporating spectral data that had undergone moving average smoothing, thereby forming an enhanced sample set. Furthermore, the probability mass and error rate of training set samples were integrated to formulate the sample weight coefficient. At last, the augmented training set was employed to establish a classification model via a three-layer neural network. Multiple experimental results showed that this method significantly improves classification performance for online sorting data, and the classification accuracy exceeds 90 %.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113990"},"PeriodicalIF":6.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Coronatine promotes post-harvest softening in ‘Gui Qi’ mango (Mangifera indica L.) through regulation of β-galactosidase and ethylene biosynthesis-related genes 冠状碱通过调节β-半乳糖苷酶和乙烯生物合成相关基因,促进“桂七”芒果收获后的软化
IF 6.8 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-10-14 DOI: 10.1016/j.postharvbio.2025.113989
Ziqing Mi , Bilal A. Rather , Hanqing Cong , Yibo Bai , Rongxiang Wang , Bo Zheng , Xuefei Jiang , Pingyin Guan , Fei Qiao
{"title":"Coronatine promotes post-harvest softening in ‘Gui Qi’ mango (Mangifera indica L.) through regulation of β-galactosidase and ethylene biosynthesis-related genes","authors":"Ziqing Mi ,&nbsp;Bilal A. Rather ,&nbsp;Hanqing Cong ,&nbsp;Yibo Bai ,&nbsp;Rongxiang Wang ,&nbsp;Bo Zheng ,&nbsp;Xuefei Jiang ,&nbsp;Pingyin Guan ,&nbsp;Fei Qiao","doi":"10.1016/j.postharvbio.2025.113989","DOIUrl":"10.1016/j.postharvbio.2025.113989","url":null,"abstract":"<div><div>Fruit softening is a key physiological event during ripening that directly influences texture, consumer acceptance, and early market supply. In ‘Gui Qi’ mango (<em>Mangifera indica</em> L.), timely and uniform softening is essential for commercial value and shelf-life management. While ethylene is well established as the primary regulator of ripening and softening in climacteric fruits, the role of coronatine (COR) in this process has not yet been investigated in ‘Gui Qi’ mango. In this study we investigated the effect of exogenous COR on mango fruit ripening and softening. It was observed that COR treatment had no effect on sugar and acid metabolism, but significantly reduced the fruit firmness and malondialdehyde (MDA) content, and increased peroxidase (POD) and catalase (CAT) activities. In addition, we have identified 24 mango β-galactosidase (<em>Miβ-GAL)</em>-encoding genes by transcriptome analysis. The results of relative gene expression showed that COR postharvest-treatment induced the expression of <em>Miβ-GAL2</em>, which exhibits a significant correlation with the ethylene level and its biosynthetic encoding genes such as S-adenosylmethionine synthetase (SAMS), 1-aminocyclopropane-1-carboxylate synthase (ACS), and aminocyclopropanecarboxylate oxidase (ACO) and synergically promote fruit softening. Therefore, treatment with COR modulates the expression of several <em>Miβ-GAL genes</em>, leading to increased β-GAL activity and a faster postharvest softening process in ‘Gui Qi’ mango.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113989"},"PeriodicalIF":6.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improvement of antioxidant activity and postharvest quality of fresh-cut artichoke by dipping in flavonoid-rich bergamot juice 浸浸富黄酮佛手柑汁提高鲜切洋蓟抗氧化活性及采后品质
IF 6.8 1区 农林科学
Postharvest Biology and Technology Pub Date : 2025-10-14 DOI: 10.1016/j.postharvbio.2025.113986
Palumbo Michela , Quintieri Laura , Bartella Lucia , Di Donna Leonardo , Santoro Ilaria , Luparelli Anna , Caputo Leonardo , Pace Bernardo , Cozzolino Rosaria , Matarazzo Cristina , Cefola Maria
{"title":"Improvement of antioxidant activity and postharvest quality of fresh-cut artichoke by dipping in flavonoid-rich bergamot juice","authors":"Palumbo Michela ,&nbsp;Quintieri Laura ,&nbsp;Bartella Lucia ,&nbsp;Di Donna Leonardo ,&nbsp;Santoro Ilaria ,&nbsp;Luparelli Anna ,&nbsp;Caputo Leonardo ,&nbsp;Pace Bernardo ,&nbsp;Cozzolino Rosaria ,&nbsp;Matarazzo Cristina ,&nbsp;Cefola Maria","doi":"10.1016/j.postharvbio.2025.113986","DOIUrl":"10.1016/j.postharvbio.2025.113986","url":null,"abstract":"<div><div>Artichoke (<em>Cynara cardunculus</em> var. <em>scolymus</em>) is a vegetable highly perishable when it is processed as fresh-cut products due to the related preparation steps which can physically alter it and favour a major risk of contamination and degradation. In this work, for the first time, fresh-cut artichoke heads were dipped in a bergamot juice extract (BE 0.05 % w/v) containing flavonoids with antimicrobial and antioxidant properties (neoeriocitrin, naringin, neohesperidin, peripolin, melitidin and brutieridin), and stored for 9 d at 5 °C. Compared to the controls, and ethanol (1 %) dipped samples, BE-dipped fresh-cut artichokes, showed improved sensory attributes, lower respiration rate and a reduction of <em>Pseudomona</em>s spoilers by 1 log CFU g<sup>−1</sup> up to 6 d of storage. Moreover, an increase in antioxidant activity was also observed in BE-dipped samples throughout the storage, putatively related to the added flavonoids (naringin, melitidin, brutieridin), which were retained up to 6 d of storage. The dipping in BE also preserved the organoleptic properties of these samples; indeed, volatile organic compounds (VOCs) profiles of artichoke dipped in BE solution showed a higher content of “green leaves” volatiles. Among these, 1-hexanol, 2-hexen-1-ol, <em>cis</em>-3-hexen-1-ol, hexanal, cis-2-hexenal, were correlated with the sensory attributes of freshness. In conclusion, in this work, a sustainable and innovative strategy based on the dipping of fresh-cut artichokes in BE proposed for the first time has showed to improve the antioxidant value and the shelf-life of the samples, preserving their sensory attributes.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113986"},"PeriodicalIF":6.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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