Postharvest Biology and Technology最新文献

{"title":"Cell wall and reactive oxygen metabolism responses of stored Shine Muscat grapes to combined melatonin and 24-epibrassinolide treatment","authors":"","doi":"10.1016/j.postharvbio.2024.113192","DOIUrl":"10.1016/j.postharvbio.2024.113192","url":null,"abstract":"<div><p>Combined treatment with melatonin (MT) and 24-epibrassinolide (EBR) is a novel compound hormone preservation technology, but there are few studies on its effects in Shine Muscat grapes. This study used Shine Muscat grapes to investigate the effects of MT and EBR co-treatment on the regulation of cell wall metabolism and reactive oxygen species. After 56 days of storage, combined treatment with MT and EBR maintained the structural integrity of the cell wall and cellular organelles, delayed the decrease in cellulose content and original pectin and the increase in soluble pectin, and inhibited the activity of cellulase, polygalacturonase, pectin methylesterase, and <em>β</em>-galactosidase. The process of cell wall metabolism was slowed down and cell wall dissolution was inhibited during storage. At the same time, MT+EBR treatment group inhibited the increase of MDA content and production rate after harvest, and increased the contents of the total phenolic and flavonoid. At the end of storage, MDA content in fruit and fruit stalk of MT+EBR group was decreased by 19.03 %, 26.05 % compared with MT groups and decreased by 21.59 % and 31.39 % compared with EBR groups. MT+EBR treatment group could not only effectively improve the hydroxyl free radical clearance rate and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging rate of grape fruit and fruit stalk during storage, but also improve superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), phenylalanine ammonia-lyase (PAL), 4-coumaric acid-coenzyme A ligase (4CL) and cinnamic acid-4-hydroxylase enzyme activity(C4H). These results suggest that the combined MT/EBR treatment can maintain grape fruit firmness by regulating fruit cell wall metabolism, slowing down cellular microstructure damage, enhancing enzymatic antioxidant defense system, delaying post-harvest senescence, and prolonging the storage period.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S092552142400437X/pdfft?md5=0094bf8c5dfb65324371032b4ca63971&pid=1-s2.0-S092552142400437X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142233741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a stable YOLOv8 classification model for apple bruising detection based on physicochemical property analysis and structured-illumination reflectance imaging","authors":"","doi":"10.1016/j.postharvbio.2024.113194","DOIUrl":"10.1016/j.postharvbio.2024.113194","url":null,"abstract":"<div><p>Effective and accurate detection of bruises at all stages has always been a challenge in non-destructive grading of apples. In this study, the visible structured-illumination reflectance imaging (SIRI) combing with deep learning method was proposed to identify bruised ‘Fuji’ apples at four different time stages (0, 6, 12 and 24 h). The macroscopic/microscopic structures and physicochemical properties of bruised tissue were measured and analyzed to determine the relationship between bruising time and these properties, as well as how they affect the accuracy of bruising detection. Results indicated that classification accuracy increased with the decrease of water and total phenolic content of the bruised tissue, as well as with the increase of color browning and bruised area. The YOLOv8 model achieved the highest detection accuracy (99.5 %) and stability. This research enhances understanding of apple bruise optics and aids in developing advanced nondestructive testing techniques.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0925521424004393/pdfft?md5=9e3e41361e3b114c86b885a4e24a9db4&pid=1-s2.0-S0925521424004393-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold tolerance of postharvest fungal pathogens is regulated by the conserved high osmolarity glycerol (HOG) pathway","authors":"","doi":"10.1016/j.postharvbio.2024.113206","DOIUrl":"10.1016/j.postharvbio.2024.113206","url":null,"abstract":"<div><p>The cold-tolerant pathogenic fungi, typically represented by the grey mold fungus <em>Botrytis cinerea</em>, can pose a serious threat to the quality and safety of postharvest fruits in the cold chain period. However, the mechanism of cold tolerance in fungal pathogens is rarely known and control measures are lacking. In this study, gene expression, genetic mutation, biochemistry, and cellular studies were carried out, revealing that low temperature could rapidly and continuously induce expression levels of the genes related to glycerolipid metabolism and HOG-MAPK pathway in <em>B. cinerea</em>. Western blot analysis showed that low temperature treatment could enhance the phosphorylation level of BcSAK1, the core component of the HOG-MAPK pathway of <em>B. cinerea</em>, and increase the proportion of BcSAK1 localization in nuclei. Deletion of BcSAK1 significantly inhibited fungal growth at low temperature. Addition of HOG-MAPK inhibitor could also inhibit nuclear localization of BcSAK1, and suppress the spore germination and mycelial growth of grey mold at low temperature. These results indicate that low temperature treatment can regulate the cold tolerance of <em>B. cinerea</em> by activating the HOG-MAPK signaling pathway. Analysis of Hog1 homologous gene deletion mutants in <em>Fusarium asiaticum</em> and <em>Alternaria alternata</em> showed that Hog1 is also essential for the cold tolerance of these two pathogenic fungi. In conclusion, this study suggests that the HOG-MAPK pathway plays a highly conserved role in regulating the cold tolerance of postharvest pathogens. Future study that aims to reveal the downstream key targets and regulatory networks of HOG-MAPK in response to cold temperature will lay foundations for the control of cold tolerant diseases of postharvest fruits.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0925521424004514/pdfft?md5=c601dbe257e342ae3f772de2a185b5b7&pid=1-s2.0-S0925521424004514-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large-scale data-driven uniformity analysis and sensory prediction of commercial banana ripening process","authors":"","doi":"10.1016/j.postharvbio.2024.113203","DOIUrl":"10.1016/j.postharvbio.2024.113203","url":null,"abstract":"<div><p>Artificial intelligence (AI) and machine learning (ML) have found prominent yet mostly academic applications in the food supply chain specifically to preserve and optimize the quality of fresh produce and achieve uniformity across the various stages of the cold chain. Nevertheless, the practical use of AI/ML for predictive analytics within real and large-scale commercial food processes, such as banana ripening, is sparse. This study proposes a novel data-driven approach tested and validated on two new large-scale datasets to automate and optimize the banana ripening process in refrigerated marine containers by successfully employing ML in uniformity analysis of the peel color and the pulp temperature of bananas based on atmospheric conditions. The results demonstrate high correlations between the gas concentrations and the uniformity of the process, suggesting that the uniformity of the peel color and the pulp temperature of fruit can be achieved by controlling the concentrations of the <em>CO</em>₂ and <em>O</em>₂ gas levels. Furthermore, this study, for the first time, achieves accurate algorithmic predictions of oxygen levels from other atmospheric variables to provide an alternative approach for continuous, improved and more cost-effective monitoring of the atmospheric conditions during ripening. A wide-range of predictive models are tested and validated where the Long Short Term Memory regression provides the lowest root-mean-square-errors (0.033 and 0.202) with robust R-squared values (0.999 and 0.959) for two datasets.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0925521424004484/pdfft?md5=c9dcde9cfbf65bf6451ef40d27efc6ea&pid=1-s2.0-S0925521424004484-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriocin paracin wx3 biosynthesis improves antagonistic activity of Lactobacillus paracasei WX322 against soft rot of postharvest pepper","authors":"","doi":"10.1016/j.postharvbio.2024.113177","DOIUrl":"10.1016/j.postharvbio.2024.113177","url":null,"abstract":"<div><p>Postharvest peppers are susceptible to soft rot caused by <em>Pectobacterium carotovorum</em> during storage and transportation. Lactic acid bacteria (LAB) are promising antagonist to control soft rot. LAB usually exhibit strong <em>in vitro</em> antibacterial activity on MRS agar plates, but weak <em>in vivo</em> disease control activity on fruit/vegetable. This study aimed to improve the biocontrol activity of <em>Lactobacillus paracasei</em> WX322 <em>in vivo</em>. Stress conditions were firstly investigated and the results showed that starvation stress could not induce any enhancement of antibacterial activity <em>in vitro</em>. Acid-base stress only improved antibacterial activity <em>in vitro</em>, while osmotic stress under 1.5 % NaCl improved both antibacterial activity <em>in vitro</em> and disease control activity <em>in vivo</em>. To facilitate the adaptation of <em>L. paracasei</em> WX322 on green pepper, pepper juice was used to replace MRS medium. To facilitate the growth of <em>L. paracasei</em> WX322 in pepper juice, nutrient supplement was studied. The result showed that pepper juice containing 0.4 % Tween 80 and 1.5 % NaCl was the optimal induction condition which improved the antagonistic activity of <em>L. paracasei</em> WX322 by enhancing LAB’s metabolism. Bacteriocin production was significantly improved under the optimal induction condition. The result of RT-qPCR revealed that bacteriocin production related genes were significantly up-regulated under the optimal induction condition, including three-component system. The addition of 1 μM autoinducer peptide (AIP) in MRS significantly increased antibacterial activity of <em>L. paracasei</em> WX322 <em>in vitro</em>. Simultaneously, <em>L. paracasei</em> WX322 cultured in MRS containing 0.01 μM AIP had a significantly increased disease control activity. RT-qPCR further verified an increased expression of bacteriocin paracin wx3 when adding 0.01 μM AIP. These results suggest that the medium of pepper juice containing 0.4 % Tween 80 and 1.5 % NaCl can improve the <em>in vivo</em> disease control activity of <em>L. paracasei</em> WX322, which is achieved by improved bacteriocin biosynthesis regulated by three-component system.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0925521424004228/pdfft?md5=f4dc21add7ce82d57215ea675a03a279&pid=1-s2.0-S0925521424004228-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomic and miRNA-seq analysis of Pak-choi provides insight into the molecular mechanisms involved in the preservation of postharvest quality by white LED light irradiation","authors":"","doi":"10.1016/j.postharvbio.2024.113183","DOIUrl":"10.1016/j.postharvbio.2024.113183","url":null,"abstract":"<div><p>Low-intensity white light-emitting diode (LED) irradiation effectively delays postharvest senescence in pak-choi at 20 °C and 90 % relative humidity. In this study, RNA-seq and miRNA-seq analyses were conducted on samples collected at 0 d (initial sample) and 5 d (CK and LED-treated samples) to evaluate the regulatory effects of white LED irradiation on miRNA. The resulting data revealed a total of 23 differentially expressed miRNAs (DEmiRNAs) in the CK/LED group on 5 d of storage. LED irradiation induced the expression of AP2 family genes and the biosynthesis of aliphatic glucosinolates by modulating the level of bra-miR172 family miRNAs. LED irradiation also stimulated the expression of genes associated with the photosynthetic pathway in pak-choi and regulated biosynthesis of ascorbic acid and resistance-associated transcription factors by modulating the level of novel miRNAs. Furthermore, LED irradiation suppressed cellulase and polygalacturonase activity, and maintained the pectin content. These findings demonstrate the ability of white LED light irradiation to significantly delay the postharvest senescence process in pak-choi by modulating miRNA expression.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0925521424004289/pdfft?md5=62703987ebe09b9f5399de144494a7ad&pid=1-s2.0-S0925521424004289-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organic fungicides and diphenylamine shift microbiomes of ‘Fuji’ apples during storage","authors":"","doi":"10.1016/j.postharvbio.2024.113196","DOIUrl":"10.1016/j.postharvbio.2024.113196","url":null,"abstract":"<div><p>The native microbiome plays an important role in biocontrol efficacy, but less is known about how the microbiome responds to conventional and organic natural product fungicides. This study investigated the effects of the conventional fungicide fludioxonil and the organic fungicide natamycin, with and without the superficial scald inhibitor diphenylamine (DPA) on the microbiomes of ‘Fuji’ apples from 1 to 28 d of storage at 0.5 °C plus 7 d at 20 °C. We hypothesized that fungicide applications would shift the microbiome, with a more pronounced effect from natamycin due to the target specificity of fludioxonil. We also predicted that the antioxidant properties of DPA would shift both bacterial and fungal microbiomes. We found that natamycin resulted in modest fungal shifts and fludioxonil resulted in no observed shifts, while DPA strongly affected the fungal microbiome over time. Chemical treatment was not a predictor of bacterial microbiome variation, but bacterial communities shifted throughout storage. However, many of the trends that occurred during storage were reversed during the 7-d shelf life period at 20 °C after storage. Time in cold storage decreased the relative abundance of <em>Pseudomonas</em>, while DPA application reduced the relative abundance of <em>Aureobasidium,</em> both notable biocontrol genera<em>.</em> These results highlight how chemical applications such as DPA may have unintended effects on beneficial microbes that protect fruit from pathogen infection.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142172404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NIR spectroscopy for quality assessment and shelf-life prediction of kiwifruit","authors":"","doi":"10.1016/j.postharvbio.2024.113201","DOIUrl":"10.1016/j.postharvbio.2024.113201","url":null,"abstract":"<div><p>In this study, a non-destructive quality testing method along with shelf-life prediction of Xu Xiang ready-to-eat kiwifruit were developed using near-infrared spectroscopy (NIR) techniques. Several traditional quality indicators (hardness, soluble solids content, and dry matter) were evaluated. Partial least squares regression (PLS) was used to predict the intrinsic quality attributes of the samples. Competitive adaptive reweighted sampling algorithm (CARS) and uninformative variable elimination (UVE) algorithm were used to select the characteristic wavelengths. Prediction models for hardness, soluble solids content and dry matter were developed. The results showed that the prediction ability of the models could be improved by screening the characteristic wavelengths of CARS and UVE. Among them, the CARS-SNV-PLS model based on soluble solids had the best prediction ability (RMSEP of 0.430 and Rp² of 0.958). Then, an NIR-based residual shelf-life prediction model was obtained by linking the measured quality indicators to the residual shelf-life, which was well validated with an RMSEP of 1.64 and an Rp² of 0.939. Therefore, this study demonstrated the potential of combining CARS, SNV, and PLS for the non-destructive testing of ready-to-eat kiwifruit to provide technical support and solution.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The SNARE protein CfSnc1 of Colletotrichum fructicola is a key regulator in fungal pathogenicity and responses to hypovirulence-associated mycovirus infection","authors":"","doi":"10.1016/j.postharvbio.2024.113190","DOIUrl":"10.1016/j.postharvbio.2024.113190","url":null,"abstract":"<div><p>Hypovirulence-associated mycoviruses not only provide new alternative for biological control, but also provide a model for studying the pathogenesis of fungal pathogens. Our previous study showed that the Colletotrichum alienum partitivirus 1 (CaPV1) is a hypovirulence-associated mycovirus which can affect vesicle transport in the host fungus. Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins play crucial roles in membrane fusion and vesicle trafficking in eukaryotes, which are essential in fungal development and virulence. Here, we identified and characterized <em>CfSNC1</em> of <em>Colletotrichum fructicola</em>, an ortholog of yeast SNARE SNC1. <em>CfSNC1</em> is down-regulated in response to infection with CaPV1. To elucidate the potential role of CfSNC1 and its involvement in the relationship between CaPV1 and <em>C. fructicola</em>, targeted gene deletion and complemented mutants were generated. Deletion of <em>CfSNC1</em> affected vegetative growth and endocytosis pathway, reduced conidiation and appressorium formation, as well as the virulence. Upon CaPV1 infection, vegetative growth of the deletion mutant was more retarded when compared to the CaPV1-infected wild type (WT). Viral RNA accumulation was significantly increased in the deletion mutant compared to that in the virus-infected WT. Taken together, this study revealed that CfSnc1 is a host factor playing roles in development and virulence of <em>C. fructicola</em> and affecting the viral RNA accumulation of CaPV1, which might offer insights into the underlying mechanisms through which mycoviruses manipulate vesicle transport to influence fungal pathogenesis.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142172403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined treatment of ɛ-polylysine and UV-C inhibited aerobic bacteria growth, and maintained the color, texture, flavor of vacuum-packed fresh-cut lettuce slices","authors":"","doi":"10.1016/j.postharvbio.2024.113156","DOIUrl":"10.1016/j.postharvbio.2024.113156","url":null,"abstract":"<div><p>In this study, combined epsilon-polylysine and ultraviolet-C (ε-PL+UV-C) treatments were used to maintain the texture and flavour of vacuum-packed fresh-cut lettuce slices during their shelf life. The results showed that vacuum packaging could effectively maintain the green surface of fresh-cut lettuce slices compared with conventional heat-sealing packaging. Aside from preventing the growth of aerobic bacteria, the ε-PL+UV-C treatment inhibited the surface discolouration of, and chlorophyll degradation within, the fresh-cut lettuce slices while also maintaining their quality and textural characteristics. Additionally, lettuce treated with ε-PL+UV-C had a better flavour than those treated with either ε-PL dipping or UV-C irradiation alone. These findings indicate that ε-PL+UV-C combined with vacuum packaging has practical application in the preservation of fresh-cut lettuce.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}