Postharvest Biology and Technology最新文献

{"title":"Insights into the changes in cell wall polysaccharides and ROS metabolism of fresh-cut chili pepper (Capsicum annuum L.) infected by Enterobacter cloacae","authors":"Zudi Li ,&nbsp;Xiaoyan Zhao ,&nbsp;Dan Wang ,&nbsp;Pan Wang ,&nbsp;Shuang Zhao ,&nbsp;Hangjun Chen ,&nbsp;Yanchao Han ,&nbsp;Wenting Zhao","doi":"10.1016/j.postharvbio.2025.113499","DOIUrl":"10.1016/j.postharvbio.2025.113499","url":null,"abstract":"<div><div><em>Enterobacter cloacae,</em> a typical spoilage bacterium, causes severe textural softening of freshly cut chili pepper. This study investigated the mechanisms of <em>E. cloacae</em>-induced textural softening involving cell wall polysaccharide disassembly and reactive oxygen species (ROS) production. It was found that <em>E. cloacae</em>-inoculated samples had lower firmness and greater weight loss than the control group. Meanwhile, <em>E. cloacae</em> inoculation promoted cell wall decomposition; cell wall polysaccharide disassembly, such as cellulose and Na₂CO₃-soluble pectin; and enhanced cell wall-degrading enzyme (CWDE) activity (polygalacturonase, pectin methylase, pectin lyase, <em>β</em>-galactosidase, <em>α</em>-L-arabinofuranosidase, cellulase, and xyloglucan endotransglycosylase). Furthermore, high H₂O₂ content, low GSH content, and reduced activities of superoxide dismutase, catalase, and ascorbate peroxidase were found in the <em>E. cloacae</em>-inoculated group during late storage. Overall, <em>E. cloacae-</em>induced textural softening of fresh-cut chili peppers resulted from cell-wall polysaccharide degradation due to increased CWDE activity, ROS accumulation, and reduced ROS-scavenging capacity.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113499"},"PeriodicalIF":6.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postharvest hypoxia and anoxia stresses delay anthocyanin accumulation in cold-stored blood orange fruit","authors":"Fariborz Habibi ,&nbsp;Moshe Doron ,&nbsp;Talia Jacobson ,&nbsp;Cătălin Voiniciuc ,&nbsp;Jeffrey K. Brecht ,&nbsp;Ali Sarkhosh","doi":"10.1016/j.postharvbio.2025.113508","DOIUrl":"10.1016/j.postharvbio.2025.113508","url":null,"abstract":"<div><div>The effect of different gas compositions on bioactive compounds, antioxidant activity, and quality of ‘Moro’ blood orange fruit during cold storage was investigated. The fruit were kept at 10 °C in hermetically closed chambers (190 L) with different gas compositions, including 1) normal air, 2) 20 % CO₂ in air, 3) 30 % CO₂ in air, 4) 20 % CO₂ in N₂, 5) 30 % CO₂ in N₂, and 6) 100 % N₂, for 21 days. Weight loss and firmness loss increased across all treatments. However, fruit stored in CO₂-enriched atmospheres exhibited significantly lower weight loss and better firmness retention compared to those stored in normal air. Total soluble solids (TSS) remained stable, but titratable acidity (TA) decreased, particularly in normal air, leading to the highest TSS/TA ratio in this treatment. Sucrose levels increased in treatments containing O₂ and decreased under O₂-depleted (anoxic) conditions by day 14. Glucose and fructose concentrations initially increased, particularly in normal air and 30 % CO₂ conditions and were lowest in the 30 % CO₂ in N₂ treatment. The highest levels of total anthocyanin content (TAC), total phenolic content (TPC), and total antioxidant activity (TAA) were observed in normal air. Storing blood oranges in normal air was most effective in enhancing TAC, TPC, TAA, and juice quality during storage. However, CO₂-enriched conditions were more effective in maintaining physical qualities. Overall, fruit quality in treatments containing O₂ was better than in those stored under O₂-depleted (anoxic) conditions, suggesting that O₂ promotes the enhancement of bioactive compounds, especially anthocyanin, in cold-stored blood oranges.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113508"},"PeriodicalIF":6.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143610623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Class III peroxidases CgPRX24/41/65 regulate postharvest lignification in granulated juice sacs during citrus fruit senescence","authors":"Mei Hu ,&nbsp;Yaci Liu ,&nbsp;Kaifang Zeng ,&nbsp;Meizhu Huang ,&nbsp;Chunlian Huang ,&nbsp;Shixiang Yao","doi":"10.1016/j.postharvbio.2025.113498","DOIUrl":"10.1016/j.postharvbio.2025.113498","url":null,"abstract":"<div><div>Granulation, a physiological disorder in citrus fruit characterized by progressive lignification of juice sacs during ripening and postharvest senescence, significantly impacts fruit quality. While class III peroxidases (PRXs) are recognized as crucial enzymes for lignin monomer polymerization, their specific regulatory roles in citrus granulation remain unclear. In this study, we performed a comprehensive analysis of the PRX gene family in <em>Citrus grandis</em> (pomelo) to explore their role in lignification during postharvest granulation. A total of 88 PRX genes were identified, and phylogenetic and expression pattern analyses revealed that four PRX genes—<em>CgPRX24</em>, <em>CgPRX41</em>, <em>CgPRX64</em>, and <em>CgPRX65</em>—were upregulated during the granulation process across multiple growing seasons. Further functional studies through transient overexpression in citrus fruit pericarp demonstrated that <em>CgPRX24</em>, <em>CgPRX41</em>, and <em>CgPRX65</em> significantly promoted lignification, enhancing pericarp firmness and lignin content in both pomelo and sweet orange. In contrast, <em>CgPRX64</em> had no significant effect on lignification. Conserved upregulation patterns and high sequence similarity (more than 93 %) of homologs in <em>Citrus sinensis</em> suggest evolutionary conservation of these PRXs' lignification function across citrus species. Our findings establish <em>CgPRX24/41/65</em> as phylogenetically conserved central regulators of juice sac lignification during postharvest citrus senescence. The present study not only functionally validates the \"Disorder of cell wall metabolism inducing granulation\" hypothesis but also provides valuable insights into the molecular mechanism underlying fruit senescence.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113498"},"PeriodicalIF":6.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hot water pre-treatment alleviates chilling injury in sweet potato: Synergistic regulatory effects on antioxidant and energy metabolism","authors":"Xudong Zhang ,&nbsp;Jinxiao Cheng ,&nbsp;Lingling Pang ,&nbsp;Di Shen ,&nbsp;Yining Xia ,&nbsp;Yanfang Pan","doi":"10.1016/j.postharvbio.2025.113514","DOIUrl":"10.1016/j.postharvbio.2025.113514","url":null,"abstract":"<div><div>This study investigated the alleviating effects of hot water (HW) treatment on chilling injury (CI) in sweet potato tuberous roots stored at 5 °C. Results showed that HW48 ℃-10 min effectively mitigated CI and browning degree during cold storage. Specially, HW48 ℃-10 min maintained cellular structural integrity, as evidenced by lower relative conductivity, poor malondialdehyde accumulation, lower lipoxygenase activity, and a more intact microstructure. Furthermore, the treatment enhanced the activities of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase, while strengthening antioxidant capacity and reducing membrane damage. Additionally, higher energy charge levels, as well as activities of cytochrome oxidase, succinate dehydrogenase, Ca^2 +-ATPase, and H⁺-ATPase, were observed in samples treated with HW48 ℃-10 min, which were positively related to membrane damage repair during cold storage. Overall, HW48 ℃-10 min would be a promising approach for preserving sweet potato tuberous roots under cold storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113514"},"PeriodicalIF":6.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1-MCP regulates taste development in cold-stored peach fruit through modulation of sugar, organic acid, and polyphenolic metabolism","authors":"Huijuan Zhou ,&nbsp;Zhengwen Ye ,&nbsp;Lufan Wang ,&nbsp;Shiying Zhang ,&nbsp;Ziyi Yuan ,&nbsp;Mingshen Su ,&nbsp;Xianan Zhang ,&nbsp;Jihong Du ,&nbsp;Xiongwei Li ,&nbsp;Minghao Zhang ,&nbsp;Yang Hu ,&nbsp;Shuling Shen ,&nbsp;Xiaolin Zheng ,&nbsp;Chen Huan","doi":"10.1016/j.postharvbio.2025.113518","DOIUrl":"10.1016/j.postharvbio.2025.113518","url":null,"abstract":"<div><div>1-Methylcyclopropene (1-MCP) shows promising application potential in cold storage preservation of fruits. Previous studies have shown that 1-MCP treatment can affect fruit flavor attributes during cold storage, with most research focusing on aroma, while studies on fruit taste are limited. This study investigated how 1-MCP treatment influences the taste profile in peach fruit during cold storage (4 ± 1 °C) and subsequent shelf life (20 ± 1 °C). Results showed that 1-MCP treatment effectively reduced chilling injury (CI) symptoms and maintained better fruit texture during storage. Electronic tongue analysis revealed that 1-MCP-treated fruit exhibited lower sweetness and bitterness, but higher sourness and umami taste compared to the control. Metabolomic analysis identified five key polyphenolic compounds (isokurarinone, isoquercetrin, catechin, 3-caffeoyl quinic acid, and 5-caffeoyl quinic acid) associated with bitterness formation, which showed strong binding affinities with human bitter taste receptors through molecular docking analysis. Transcriptomic analysis demonstrated that 1-MCP regulated fruit taste by modulating multiple metabolic pathways: suppressing sucrose degradation while promoting hexose phosphorylation in sugar metabolism, enhancing organic acid synthesis through upregulation of TCA cycle-related genes, and inhibiting phenylpropanoid metabolism pathway genes to reduce polyphenolic compound accumulation. Although 1-MCP effectively maintained fruit quality and reduced bitterness development during cold storage, the decreased sweetness might also affect consumer acceptance. Further research is needed to optimize 1-MCP treatment conditions for a better balance between preservation effects and taste formation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113518"},"PeriodicalIF":6.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of exogenous gibberellic acid on chlorophyll metabolism of Sudachi (Citrus sudachi Hort.) after harvest","authors":"Chisato Torimoto ,&nbsp;Gang Ma ,&nbsp;Zhiwei Deng ,&nbsp;Lancui Zhang ,&nbsp;Masaki Yahata ,&nbsp;Saya Ojima ,&nbsp;Masaya Kato","doi":"10.1016/j.postharvbio.2025.113476","DOIUrl":"10.1016/j.postharvbio.2025.113476","url":null,"abstract":"<div><div>Sudachi (<em>Citrus sudachi</em> Hort.), cultivated in Japan, is a type of sour citrus fruit. Sudachi fruit is preferred by consumers for its rich flavor during the green period of the peel. As the Sudachi fruit ripens, the flavedo color changes from green to yellow and the fruit loses its flavor. In the present study, to inhibit the color development in the flavedo of Sudachi, the fruit were dipped in 500 μM of gibberellic acid (GA) solution for 1 h, and the effects on chlorophyll metabolism were investigated in Sudachi fruit. The results indicated that the GA treatment delayed the degreening, and inhibited the decrease in chlorophyll contents in the flavedo of Sudachi. With GA treatment, the expression levels of chlorophyll biosynthetic genes (<em>CitGUN4</em>, <em>CitCHLH</em>, <em>CitPORA</em>, <em>CitCHL27</em>, and <em>CitGGDR</em>) were upregulated and chlorophyll catabolic genes (<em>CitNYC</em>, <em>CitPPH</em>, <em>CitSGR</em>, and <em>CitPAO</em>) were downregulated, which led to preserving a high levels of chlorophyll contents. The results of this study elucidated the molecular mechanism of the GA effect on citrus fruit, which suggested that GA effectively inhibited the yellowing of citrus flavedo.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113476"},"PeriodicalIF":6.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis of Frequency Learning Networks combined with co-depth point localization identification to screen and validate efficient preservation methods for pitaya: 1-methylcyclopropene, carvacrol and gibberellic acid","authors":"Geng Liu ,&nbsp;Jieyu Li ,&nbsp;Xiaoyu Hong ,&nbsp;Ziqin Bai ,&nbsp;Qing Tan ,&nbsp;Chenlan Li ,&nbsp;Qiqiao Pan ,&nbsp;Hui Luo ,&nbsp;Wei Xue","doi":"10.1016/j.postharvbio.2025.113493","DOIUrl":"10.1016/j.postharvbio.2025.113493","url":null,"abstract":"<div><div>This study verified the feasibility of Meta-analysis combined with co-depth point localization identification in screening plant preservation methods and innovative joint-use preservation strategies, pointed out the reasons for the deviation, optimized the computational method of network Meta-analysis, and explored the effectiveness of the combined treatments screened under the combination of the two approaches in practical application. A Bayesian random-effects model with Meta-analysis of Frequency Learning Networks (FLN) was used to compare the freshness preservation effects of pitaya under 28 different interventions, and the best interventions were screened for a single quality index of pitaya during the storage period. We combined the two treatments with the highest weights after the comparison and found, both experimentally and after validation with co-depth point localization identification, that the 1-methylcyclopropene (1-MCP) + carvacrol (CVR) coupled with gibberellic acid (GA₃) soaking was more effective in suppressing postharvest pitaya decay and maintaining quality during storage than 1-MCP + CVR treatment alone or soaking with GA₃.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113493"},"PeriodicalIF":6.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SwishFormer for robust firmness and ripeness recognition of fruits using visual tactile imagery","authors":"Mashood M. Mohsan ,&nbsp;Basma B. Hasanen ,&nbsp;Taimur Hassan ,&nbsp;Muhayy Ud Din ,&nbsp;Naoufel Werghi ,&nbsp;Lakmal Seneviratne ,&nbsp;Irfan Hussain","doi":"10.1016/j.postharvbio.2025.113487","DOIUrl":"10.1016/j.postharvbio.2025.113487","url":null,"abstract":"<div><div>The accurate assessment of fruit ripeness is a critical task in the agricultural industry. It affects the fruit quality, shelf-life, and consumer satisfaction. Traditional methods for estimating fruit ripeness rely on subjective human judgment and invasive sampling techniques, which are both infeasible and time-consuming. This paper presents a novel method for estimating firmness and ripeness of fruits using their palpation motion encoded within the visual tactile scans. Moreover, these tactile scans are passed to the proposed SwishFormer model coupled with Random Forest head to predict the fruits firmness, which is later used in classifying the fruits ripeness stage. SwishFormer, unlike the existing state-of-the-art models, encompasses hardswish activation as a token mixer which allows it to generate distinctive set of features from the candidate tactile scans. These rich feature representations are then fed to the Random Forest regressor to robustly estimate the fruit firmness values and the estimated firmness values are then used in accurately predicting the ripeness level of the fruits. Apart from this, SwishFormer is extensively evaluated on the proposed dataset, containing the palpation visual tactile scans, and it outperforms state-of-the-art works by achieving 4.77%, 4.09%, 13.69%, and 4.65% better performance in terms of MSE, RMSE, R<span><math><msup><mrow></mrow><mrow><mn>2</mn></mrow></msup></math></span>, and MAE scores, while possessing 2.02 times less parameters, and 2.09 times lesser GMACs. Additionally, the ripeness recognition performance of the proposed system is thoroughly tested through real-world experiments using a Stretch Robot, where it achieves a success rate of 96.6%, 98.3%, and 93.3% for recognizing avocados as underripe, ripe, and overripe, respectively. To the best of our knowledge, this paper introduces a first non-destructive approach to estimate fruit firmness and ripeness using off-the-shelf vision-based tactile information. Moreover, the proposed dataset and source code of this work is available at <span><span>https://mashood3624.github.io/SwishFormer/</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113487"},"PeriodicalIF":6.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing composite biocontrol agents based on microbiome dynamics during postharvest storage of tomatoes","authors":"Lu Zhuang ,&nbsp;Xiaoyun Zhang ,&nbsp;Solairaj Dhanasekaran ,&nbsp;Esa Abiso Godana ,&nbsp;Jun Li ,&nbsp;Ruijie Luo ,&nbsp;Lina Zhao ,&nbsp;Yu Li ,&nbsp;Xiaoyong Liu ,&nbsp;Hongyin Zhang","doi":"10.1016/j.postharvbio.2025.113509","DOIUrl":"10.1016/j.postharvbio.2025.113509","url":null,"abstract":"<div><div>Single antagonistic microorganisms introduced into the microbial community on the fruit surface often show poor adaptation, resulting in less efficient and inconsistent pathogen inhibition, whereas the interaction of composite microorganisms can increase their ability to combat plant diseases. This study used high-throughput sequencing techniques to explore the dynamics of bacterial and fungal communities on the surface of postharvest tomatoes during storage. Subsequently, potentially beneficial core microorganisms were identified based on correction correlations with one another, and microbial formulations were developed using the isolated beneficial core microorganisms with control efficacy. Based on the microbiome analysis results, two pathogens were screened and identifi<em>ed</em> as <em>Alternaria tenuissima</em> and <em>Cladosporium cladosporioides.</em> Among the five potentially beneficial core microorganisms that were isolated, <em>Bacillus velezensis</em> B1, <em>Pantoea agglomerans</em> B10, and <em>Meyerozyma caribbica</em> Y9, demonstrated safety and highest control efficacy. Considering the affinity between strains, <em>M. caribbica</em> Y9 was combined with either <em>P. agglomerans</em> B10 or <em>B. velezensis</em> B1 to formulate composite microorganisms. The <em>in vivo</em> results demonstrated that all composite microorganisms were more effective in controlling the postharvest diseases caused by <em>A. tenuissima</em> and <em>C. cladosporioides</em> than the single microorganism and <em>P. agglomerans</em> B10 and <em>M. caribbica</em> Y9 at the ratio of 2:1 being the most effective. Therefore, the composite microorganisms formulated with the isolated strains from fresh tomatoes could significantly enhance the control effect, demonstrating strong potential for inhibiting postharvest diseases in tomatoes and promising prospects for widespread application.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113509"},"PeriodicalIF":6.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo transcriptome assembly and analysis unveil molecular insights into Cladosporidium rot development in harvested table grapes","authors":"Solairaj Dhanasekaran,&nbsp;Michael Ackah,&nbsp;Qiya Yang,&nbsp;Hongyin Zhang","doi":"10.1016/j.postharvbio.2025.113497","DOIUrl":"10.1016/j.postharvbio.2025.113497","url":null,"abstract":"<div><div>Cladosporium cladosporioides is a fungal pathogen that affects various crops worldwide, including table grapes, where it causes Cladosporium rot. The molecular episodes of Cladosporium rot development are still unclear and need to be explored. More detailed knowledge of the molecular mechanisms underlying Cladosporium rot will provide upcoming developments on disease resistance and management. Here, we report RNA sequencing and transcriptomic analysis of C. cladosporioides infecting postharvest table grapes and its relationship with the grape’s defense response. High-throughput sequencing of cDNA libraries following de novo transcriptome assembly produced 70 thousand transcripts. This study is the first attempt to explore the transcriptional level changes of the fungus during Cladosporium rot development in table grapes. Our study showed that the fungus expresses huge sets of pathogenicity-related genes and families of genes encoding CAZymes, transcription factors and secretory proteins. Further, we identified genes of this fungus that facilitate nutrient uptake, metabolism and growth in grapes, which were significantly upregulated. This study provides valuable insights into the complex pathogenic mechanisms of C. cladosporioides, in which the fungi alter its transcriptome and develop adaptations to overcome the grape’s defenses and colonize table grape tissue.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113497"},"PeriodicalIF":6.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}