Postharvest Biology and Technology最新文献_第2页

DCC and ADP regulate the pulp breakdown in Phomopsis longanae Chi-inoculated longan via modulating energy levels and the metabolisms of reactive oxygen species and membrane lipids DCC和ADP通过调节能量水平、活性氧和膜脂代谢来调节龙眼接种后的果肉分解

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-26 DOI: 10.1016/j.postharvbio.2025.113662

Jin Lin , Yunman Chen , Meiqi Huang , Huili Zhang , Huafeng Hong , Mengshi Lin , Ruiling Zhuo , Yihui Chen , Hetong Lin , Yifen Lin

{"title":"DCC and ADP regulate the pulp breakdown in Phomopsis longanae Chi-inoculated longan via modulating energy levels and the metabolisms of reactive oxygen species and membrane lipids","authors":"Jin Lin , Yunman Chen , Meiqi Huang , Huili Zhang , Huafeng Hong , Mengshi Lin , Ruiling Zhuo , Yihui Chen , Hetong Lin , Yifen Lin","doi":"10.1016/j.postharvbio.2025.113662","DOIUrl":"10.1016/j.postharvbio.2025.113662","url":null,"abstract":"<div><div>Compared to <em>P. longanae</em>-inoculated longan, DCC + <em>P. longanae</em>-inoculated longan exhibited an elevated pulp breakdown index and higher cell membrane permeability, along with the reduced ATP levels and energy charge. Additionally, DCC + <em>P. longanae</em>-inoculated longan exhibited higher activities of membrane lipid-degrading enzymes (MLDEs), including PI-PLC, PC-PLC, PLD, DGK, PAP, lipase, and LOX, as well as higher levels of DAG, PA and SFAs. Conversely, the contents of PI, PC and USFAs were reduced, accompanied by lower U/S and IUFA. Besides, DCC + <em>P. longanae</em>-inoculated longan displayed lower activities of SOD, CAT, APX, GPX, MDHAR, DHAR, and GR, along with lower reducing power and DPPH radical scavenging ability, lower levels of AsA and GSH, and higher levels of DHA, GSSG, H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>·−</sup> production rate, and MDA. These outcomes indicate that DCC treatment expedited pulp breakdown in <em>P. longanae</em>-inoculated longan by exacerbating energy deficiency, which activated MLDEs activities and damaged cell membrane structure. Furthermore, the insufficient energy supply also impaired ROS-scavenging capacity, thereby leading to ROS accumulation, membrane lipid peroxidation, and loss of cell membrane integrity. In contrast, ADP + <em>P. longanae</em>-inoculated longans exhibited the opposite effects. The results of present work can provide new insight into the mechanism of longan pulp breakdown, and offer new strategies such as the application of ADP for suppressing longan pulp breakdown to extend the shelf life of fresh longan.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113662"},"PeriodicalIF":6.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative transcriptomics unveils nitric oxide delaying leaf senescence in Chinese flowering cabbage by enhancing BrGATA21-mediated ROS scavenging 比较转录组学揭示了一氧化氮通过增强brgata21介导的ROS清除来延缓白菜叶片衰老

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-24 DOI: 10.1016/j.postharvbio.2025.113673

Zhen-liang Mou , Ze-xiang Zeng , Zong-li Liu , Wei Wei , Wei Shan , Jian-fei Kuang , Wang-jin Lu , Jian-ye Chen , Ya-ting Zhao

{"title":"Comparative transcriptomics unveils nitric oxide delaying leaf senescence in Chinese flowering cabbage by enhancing BrGATA21-mediated ROS scavenging","authors":"Zhen-liang Mou , Ze-xiang Zeng , Zong-li Liu , Wei Wei , Wei Shan , Jian-fei Kuang , Wang-jin Lu , Jian-ye Chen , Ya-ting Zhao","doi":"10.1016/j.postharvbio.2025.113673","DOIUrl":"10.1016/j.postharvbio.2025.113673","url":null,"abstract":"<div><div>This study aimed to investigate the physiological and molecular effects of nitric oxide (NO) on leaf senescence in postharvest Chinese flowering cabbage. NO treatment significantly inhibited chlorophyll degradation and maintained reactive oxygen species (ROS) homeostasis, as evidenced by higher chlorophyll content and reduced ROS accumulation, accompanying with enhanced activities of ROS scavenging enzymes such as SOD, CAT and POD. Comparative transcriptomic analysis identified 11,039 differentially expressed genes (DEGs), including 848 transcription factors (TFs). Moreover, a co-expression network was constructed, highlighting 13 ROS scavenging enzyme-encoding genes, including <em>BrSOD</em>, <em>BrCAT2</em>, <em>BrPOD1,</em> and <em>BrPOD42</em>, and 28 TFs involved in NO-regulated ROS scavenging. Further investigation showed that NO-inducible BrGATA21, an important regulator in this network, directly bound to the promoters of <em>BrSOD</em>, <em>BrCAT2</em>, <em>BrPOD1,</em> and <em>BrPOD42</em>, and enhanced their expression, thereby contributing to alleviation of oxidative stress and NO-delayed leaf senescence. In summary, these findings reveal that NO acts as a negative regulator of leaf senescence in postharvest Chinese flowering cabbage, at least partially through enhancing BrGATA21-mediated ROS scavenging. The findings offer valuable insights into mechanisms underlying NO-delayed leaf senescence, providing a foundation for the application of NO in vegetable preservation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113673"},"PeriodicalIF":6.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous application of CaCl2 and GA3 alleviates the juice sacs granulation in ‘Harumi’ fruit during storage 外源施用CaCl2和GA3可减轻“春美”果实贮藏过程中汁囊的肉芽化

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-24 DOI: 10.1016/j.postharvbio.2025.113677

Zhiwei Deng , Gang Ma , Lancui Zhang , Ayami Hisanaga , Fumitaka Takishita , Keisuke Nonaka , Masaya Kato

{"title":"Exogenous application of CaCl2 and GA3 alleviates the juice sacs granulation in ‘Harumi’ fruit during storage","authors":"Zhiwei Deng , Gang Ma , Lancui Zhang , Ayami Hisanaga , Fumitaka Takishita , Keisuke Nonaka , Masaya Kato","doi":"10.1016/j.postharvbio.2025.113677","DOIUrl":"10.1016/j.postharvbio.2025.113677","url":null,"abstract":"<div><div>Juice sacs granulation is a serious physiological disorder that frequently arises during the storage of citrus fruit, which damages fruit edibility, affects consumer preferences, and losses the economy of the citrus industry. ‘Harumi’ ((<em>Citrus unshiu</em> × <em>C</em>. <em>sinensis</em>) × <em>C</em>. <em>reticulate</em>) is a late-ripening mandarin-type variety prone to juice sacs granulation during storage. Therefore, exploring measures to suppress postharvest granulation of ‘Harumi’ fruit is important. In the present study, to improve the quality of ‘Harumi’ fruit during the storage process, the effects of exogenous application of CaCl<sub>2</sub> and gibberellin A<sub>3</sub> (GA<sub>3</sub>) on the lignin and flavonoid accumulation were investigated. The results showed that both CaCl<sub>2</sub> and GA<sub>3</sub> treatments delayed the development of granulation in ‘Harumi’ fruit during storage. With the CaCl<sub>2</sub> and GA<sub>3</sub> treatments, the accumulation of lignin and flavonoid was inhibited in ‘Harumi’ fruit during storage. In addition, the results in gene expression showed that the changes in the lignin and flavonoid metabolisms in the CaCl<sub>2</sub> and GA<sub>3</sub> treatments were highly regulated at the transcriptional level. The expression levels of <em>CitPAL</em>, <em>Cit4CL</em>, <em>CitHCT</em>, <em>CitC3′H</em>, <em>CitCCR</em>, <em>CitCAD6</em>, and <em>CitPRX</em> were significantly down-regulated in CaCl<sub>2</sub> and GA<sub>3</sub>-treated fruit during storage. The results presented in this study suggested that the application of CaCl<sub>2</sub> and GA<sub>3</sub> could potentially be used to improve fruit quality during storage and explained the inhibition of lignin overaccumulation by CaCl<sub>2</sub> and GA<sub>3</sub> during citrus granulation at the molecular metabolic level.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113677"},"PeriodicalIF":6.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous melatonin activates BoNAC52-BoRBOHF module and antioxidant system involved in the delay in broccoli yellowing during postharvest 外源性褪黑素激活BoNAC52-BoRBOHF模块和抗氧化系统，参与延缓采后西兰花变黄

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-24 DOI: 10.1016/j.postharvbio.2025.113675

Yue Wang , Lufan Wang , Yuting Li , Wenbo Lei , Jianhui Cheng , Yuan Cheng , Shuling Shen , Lanying Yao , Lichao Zhou , Yan Liu , Xiaolin Zheng , Chen Huan

{"title":"Exogenous melatonin activates BoNAC52-BoRBOHF module and antioxidant system involved in the delay in broccoli yellowing during postharvest","authors":"Yue Wang , Lufan Wang , Yuting Li , Wenbo Lei , Jianhui Cheng , Yuan Cheng , Shuling Shen , Lanying Yao , Lichao Zhou , Yan Liu , Xiaolin Zheng , Chen Huan","doi":"10.1016/j.postharvbio.2025.113675","DOIUrl":"10.1016/j.postharvbio.2025.113675","url":null,"abstract":"<div><div>For further understanding the effect of melatonin (MT) treatment on delaying postharvest yellowing in broccoli through regulation of reactive oxygen species (ROS) accumulation, this study investigated changes in the antioxidant system and the BoNAC52-BoRBOHF module. Broccoli heads were treated with a 200 μmol L<sup>−1</sup> MT solution and stored at 25 °C for 4 days. The results showed that MT treatment delayed yellowing, reduced the levels of H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>·-</sup>, and MDA, and improved the antioxidant system activity in broccoli compared to the control during storage. Transcriptomic analysis identified the BoNAC52-BoRBOHF module as a key regulator of ROS levels during postharvest senescence. MT treatment upregulated <em>BoNAC52</em> expression and suppressed the expressions of four BoRBOHs, correlating with decreased ROS levels in broccoli during storage. Further investigation confirmed that BoNAC52 directly binds to the promoter of <em>BoRBOHF</em>. The regulatory role of the BoNAC52-BoRBOHF module in ROS production related to leaf senescence was confirmed through transient silencing and expression of <em>BoNAC52</em> in tobacco and pak choi. These findings demonstrate that MT treatment delays broccoli senescence and yellowing during postharvest storage by enhancing the antioxidant ability and activating the BoNAC52-BoRBOHF module to control ROS accumulation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113675"},"PeriodicalIF":6.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of storage temperatures and forchlorfenuron treatment concentrations on physiological quality and primary metabolites in kiwifruit during storage 贮藏温度和处理浓度对猕猴桃贮藏期间生理品质和主要代谢物的影响比较分析

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-23 DOI: 10.1016/j.postharvbio.2025.113665

Xiaofan Qi , Chengkui Qiao , Lijun Han , Ming Zhuang , Mengyuan Cao , Caixia Wang , Linlin Guo

{"title":"Comparative analysis of storage temperatures and forchlorfenuron treatment concentrations on physiological quality and primary metabolites in kiwifruit during storage","authors":"Xiaofan Qi , Chengkui Qiao , Lijun Han , Ming Zhuang , Mengyuan Cao , Caixia Wang , Linlin Guo","doi":"10.1016/j.postharvbio.2025.113665","DOIUrl":"10.1016/j.postharvbio.2025.113665","url":null,"abstract":"<div><div>Forchlorfenuron (CPPU) is a widely used plant growth regulator in kiwifruit production. During storage, kiwifruit requires postharvest processing, including after-ripening, preservation, and distribution, making the storage period a critical factor affecting the fruit quality. To investigate the impact of CPPU on the fruit quality during the storage period, this study used Xuxiang kiwifruits as the research material. The kiwifruits were immersed in solutions of different concentrations of CPPU 10 d after the blossom fall. Growth parameters were determined during fruit development. After the physiological maturity, the harvested kiwifruits were stored at 1 °C and 20 °C for research purposes, studying the changes in physiological quality and metabolites during storage. The results showed that CPPU promoted the accumulation of sugars and soluble solid content (SSC) at 20 °C, while it mainly reduced the content of organic acids at 1 °C. Lower concentrations of CPPU reduced fruit pigment levels, while moderate concentrations were more beneficial for maintaining the antioxidant capacity of the kiwifruits. The study's results will provide a theoretical reference for the rational use of CPPU in kiwifruit production and will also assist in quality management of postharvest kiwifruits.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113665"},"PeriodicalIF":6.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxalic acid-tea saponin complex preserves freshness and inhibits iron-polyphenol-mediated blue stain in postharvest lotus rhizomes 草酸-茶皂素复合物对采后莲藕的保鲜和抑制铁多酚介导的蓝染

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-23 DOI: 10.1016/j.postharvbio.2025.113676

Xianqiang Chen , Xiaoling Zeng , Zhongyi Liu , Siyang Li , Zongyu Liu , Yassin Haran , Jie Li , Shoulei Yan

{"title":"Oxalic acid-tea saponin complex preserves freshness and inhibits iron-polyphenol-mediated blue stain in postharvest lotus rhizomes","authors":"Xianqiang Chen , Xiaoling Zeng , Zhongyi Liu , Siyang Li , Zongyu Liu , Yassin Haran , Jie Li , Shoulei Yan","doi":"10.1016/j.postharvbio.2025.113676","DOIUrl":"10.1016/j.postharvbio.2025.113676","url":null,"abstract":"<div><div>The study investigated a novel blue stain inhibitor (BSI) on the fresh-keeping effect of lotus rhizome and explored the mechanism that prevented the occurrence of blue stain. The BSI, consisting of 0.3 % oxalic acid, 0.35 % edetate disodium, 0.15 % vitamin C and 1 % tea saponin, demonstrated effective preservation, significantly reducing the degradation of phenolic compounds, protein, reducing sugar and soluble solid and inhibiting the outgrowth of microorganisms during the 60-day storage at 4 ℃. The relative electrolyte leakage findings also suggested that Group 3 significantly lowered the electrolyte leakage from lotus rhizomes when compared to the control group (CK1). Moreover, the spectral results showed that the bluish color in lotus rhizome was primarily caused by chelates formed when <em>L</em>-dopa, gallocatechin, epigallocatechin, catechin and epicatechin bind with Fe<sup>3 +</sup>. The correlation analysis revealed that both electrolyte leakage and total bacteria count were significantly negatively correlated with <em>b</em>* (<em>p</em> < 0.05), suggesting bacteria may considerably influence the leakage of phenolic substances during storage, affecting the blue transformation of lotus rhizomes. SEM and TEM analyses showed that the BSI preservative effectively protected cell walls and membranes compared to CK1. The function of BSI was to prevent the release of phenolic compounds triggered by microorganisms, reducing the chelation of polyphenols with iron. This research is beneficial for the color protection and quality maintenance of lotus rhizomes after harvest and is also conducive to the processing of lotus rhizomes.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113676"},"PeriodicalIF":6.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SlNAP2 promotes tomato fruit ripening by directly binding the SlACS2 promoter and interacting with SlEIL3 SlNAP2通过直接结合SlACS2启动子并与slil3相互作用促进番茄果实成熟

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-23 DOI: 10.1016/j.postharvbio.2025.113659

Xuetong Wu , Hua Fang , Dengjing Huang , Xuemei Hou , Yali Qiao , Changxia Li , Ailing Li , Yi Huang , Zhiya Liu , Yayu Liu , Weibiao Liao

{"title":"SlNAP2 promotes tomato fruit ripening by directly binding the SlACS2 promoter and interacting with SlEIL3","authors":"Xuetong Wu , Hua Fang , Dengjing Huang , Xuemei Hou , Yali Qiao , Changxia Li , Ailing Li , Yi Huang , Zhiya Liu , Yayu Liu , Weibiao Liao","doi":"10.1016/j.postharvbio.2025.113659","DOIUrl":"10.1016/j.postharvbio.2025.113659","url":null,"abstract":"<div><div>The ripening process of tomato fruit is affected by a variety of environmental factors and genetic regulators. NAM-ATAF1/2-CUC2 (NAC) transcription factors (TFs) function in a multitude of biological processes, while the current knowledge on the participation of NAC TFs in the regulatory network of fruit ripening is relatively limited. In this study, we isolated a NAC TF, SlNAP2, which acts as a transcription activator in tomato fruit ripening. We also observed a notable delay in the ripening process of <em>SlNAP2</em> silencing and knockout mutant fruit. In particular, ethylene production was obviously inhibited in <em>SlNAP2</em> mutant fruit. Y1H and DLR assays showed that <em>SlNAP2</em> directly binds to the promoter of <em>SlACS2</em> and activates its transcriptional activity. Furthermore, SlNAP2 and SlEIL3 physical interaction in <em>vivo</em> and <em>vitro</em> was demonstrated. The results also found that SlEIL3 interacted with SlNAP2 to enhance the transcriptional activity of <em>SlACS2</em> and the function of ethylene in tomato fruit ripening. Collectively, our data might contribute to understanding the interaction of NAC TFs and ethylene in tomato fruit ripening, thereby enhancing our knowledge of the ripening regulatory network that governs tomato fruit maturation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113659"},"PeriodicalIF":6.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High efficacy of (Z)-ligustilide in inhibiting postharvest apple ring rot and its antifungal mechanism against Botryosphaeria dothidea (Z)-藁本内酯对苹果采后环腐病的高效抑制作用及其抑菌机理研究

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-23 DOI: 10.1016/j.postharvbio.2025.113671

Xiao-Yi Tong , Li-Li Song , Hui-Fang Du , Ya-Hui Zhang , Jia-Wen Shou , Cai-Juan Zheng , Wen-Bin Gao , Zhong-Cheng Liu , Du-Qiang Luo , Yun-Feng Liu , Fei Cao

{"title":"High efficacy of (Z)-ligustilide in inhibiting postharvest apple ring rot and its antifungal mechanism against Botryosphaeria dothidea","authors":"Xiao-Yi Tong , Li-Li Song , Hui-Fang Du , Ya-Hui Zhang , Jia-Wen Shou , Cai-Juan Zheng , Wen-Bin Gao , Zhong-Cheng Liu , Du-Qiang Luo , Yun-Feng Liu , Fei Cao","doi":"10.1016/j.postharvbio.2025.113671","DOIUrl":"10.1016/j.postharvbio.2025.113671","url":null,"abstract":"<div><div>Apple fruits, widely consumed worldwide, are highly vulnerable to postharvest infections, with <em>Botryosphaeria dothidea</em>, the primary pathogen causing apple ring rot, leading to substantial economic losses. In this study, (<em>Z</em>)-ligustilide (LIG), a compound isolated from <em>Ligusticum jeholense</em>, exhibited high efficacy against the growth of <em>B. dothidea</em>, with an IC<sub>50</sub> value of 43.1 nL/mL. Activity using detached apple fruits assay further showed that LIG effectively suppressed fungal growth across different apple cultivars, while preserving fruit quality and flavor. Scanning and transmission electron microscopy revealed significant structural damage to <em>B. dothidea</em> cells following LIG treatment. Proteomic analysis and molecular docking suggested that LIG may inhibit <em>B. dothidea</em> by disrupting its energy metabolism, with the potential targets of acetyl-CoA synthetase, MDH, and CA synthase. Additional assays, including fluorescence staining, relative electrical conductivity, malondialdehyde levels, hydrogen peroxide content, ATPase activity, and ATP concentration, provided further insights into the antifungal mechanism of LIG. These findings highlight the potential of LIG as a promising plant-derived antifungal agent in the postharvest management of ring rot on apple fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113671"},"PeriodicalIF":6.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spermidine treatment delays postharvest senescence of prune (Prunus domestica L.) fruit by regulating reactive oxygen species and membrane lipid metabolism 亚精胺处理通过调节活性氧和膜脂代谢延缓李子采后果实衰老

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-21 DOI: 10.1016/j.postharvbio.2025.113670

Xinling Zhang , Weida Zhang , Yuxing Liu , Wanting Yang , Jiankang Cao , Minrui Guo , Guogang Chen

{"title":"Spermidine treatment delays postharvest senescence of prune (Prunus domestica L.) fruit by regulating reactive oxygen species and membrane lipid metabolism","authors":"Xinling Zhang , Weida Zhang , Yuxing Liu , Wanting Yang , Jiankang Cao , Minrui Guo , Guogang Chen","doi":"10.1016/j.postharvbio.2025.113670","DOIUrl":"10.1016/j.postharvbio.2025.113670","url":null,"abstract":"<div><div>Spermidine (Spd), a membrane stabilizer and free radical scavenger, plays a pivotal role in delaying post-harvest fruit senescence. Herein, prune fruit was treated with Spd to explore its impact on fruit quality, with D-arginine (D-arg), a polyamine inhibitor, utilized as a negative control. We focused on analyzing the regulatory effects of both treatments on reactive oxygen species (ROS) and membrane lipid metabolism in prune fruit. The results showed that Spd reduced respiration rate and weight loss while maintaining high levels of firmness, soluble solid content (SSC), and titratable acid (TA). Spd maintained elevated levels of ascorbic acid (AsA) and glutathione (GSH), enhanced ROS scavenging enzyme activities and antioxidant capacity, thereby mitigating ROS-induced oxidative damage to cell membranes. Moreover, Spd inhibited the activities of phospholipid-degrading enzymes while sustaining elevated fatty acid desaturase (FADS) activity. Thus, Spd increased the levels of phosphatidylcholine (PC), phosphatidylinositol (PI), unsaturated fatty acids (USFAs), unsaturated fatty acid index (IUFA), and the ratio of unsaturated fatty acids to saturated fatty acids (U/S). Concurrently, Spd inhibited the accumulation of oxidative stress products and saturated fatty acids (SFAs), mitigated the increase in cell membrane permeability (CMP), thereby sustaining the structural and morphological integrity of mitochondria and cellular membranes in prune fruit. In contrast, D-arg treatment yielded diametrically opposite results. These results indicate that Spd delays prune fruit senescence by regulating ROS and membrane lipid metabolism.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113670"},"PeriodicalIF":6.4,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tolerance mechanism of pear to hypoxic storage: A transcriptome study 梨对低氧贮藏的耐受机制：转录组研究

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2025-05-20 DOI: 10.1016/j.postharvbio.2025.113668

Alexxandra J. Ty , Maarten L.A.T.M. Hertog , Bart Nicolaï

{"title":"Tolerance mechanism of pear to hypoxic storage: A transcriptome study","authors":"Alexxandra J. Ty , Maarten L.A.T.M. Hertog , Bart Nicolaï","doi":"10.1016/j.postharvbio.2025.113668","DOIUrl":"10.1016/j.postharvbio.2025.113668","url":null,"abstract":"<div><div>Postharvest pear (<em>Pyrus communis</em> L.) is often stored at low temperatures under an atmosphere with low O<sub>2</sub> and high CO<sub>2</sub> partial pressure (‘controlled atmosphere storage’, CA). While this condition helps to maintain fruit quality and prolong storage life, how the long-term hypoxia impacts the pear transcriptome is unknown. This study aimed to explore changes in the pear transcriptome associated with CA storage using RNA-sequencing. ‘Conference’ pears were stored for 6 months at either CA or regular air. Over 270 million paired-end reads were mapped onto the <em>Pyrus communis</em> ‘Bartlett’ DH Genome and differences between the expression profiles of CA-stored and regular air-stored fruits were assessed. Genes involved in energy status, redox state, secondary metabolism, and lipid biosynthesis were highly altered in hypoxic pears. Additionally, differentially expressed genes (DEG) related to cell senescence, apoptosis, autophagy, and plant hormone signaling were detected. This study is the first to highlight the potential involvement of crucial programmed cell death (PCD) factors, including vacuolar processing enzyme, metacaspase, and substilase, in CA-stored pear fruit. By integrating the pathways of these DEGs into a conceptual model, a coping mechanism for ‘Conference’ pear to long-term hypoxic-CA storage is proposed. These new markers might help to reveal the molecular causes of storage disorders triggered by low O<sub>2</sub> conditions.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"228 ","pages":"Article 113668"},"PeriodicalIF":6.4,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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