Postharvest Biology and Technology最新文献

{"title":"Atomized fumigation with basil essential oil regulated reactive oxygen metabolism and respiratory metabolism to maintain storage quality of blueberry fruit","authors":"Yajun Wang ,&nbsp;Shuang Min ,&nbsp;Tian Gao ,&nbsp;Canying Li ,&nbsp;Yonghong Ge","doi":"10.1016/j.postharvbio.2025.113418","DOIUrl":"10.1016/j.postharvbio.2025.113418","url":null,"abstract":"<div><div>This study was performed to explore the impact of atomized fumigation with basil essential oil (BEO) on the metabolism of reactive oxygen species, respiration, and energy, as well as quality parameters in blueberry fruit. Results demonstrated that BEO had minimal impact on surface color, significantly inhibited respiratory rate and ethylene release, while maintaining ascorbic acid, reducing sugar, soluble sugar, and reduced glutathione levels in blueberries. Moreover, BEO attenuated weight loss increase and inhibited hydrogen peroxide and malondialdehyde accumulation. BEO resulted in enhanced activities and gene expressions of ascorbate peroxidase, NADPH hydrogenase, superoxide dismutase, peroxidase, glutathione reductase, cytochrome C oxidase, hexokinase, glucose-phosphate isomerase, succinate dehydrogenase, and nicotinamide adenine dinucleotide kinase. Meanwhile, BEO up-regulated <em>VcH</em>^<em>+</em><em>-ATPase</em> and <em>VcCa</em>^<em>2+</em><em>-ATPase</em> expressions, as well as enhanced catalase, glucose-6-phosphate dehydrogenase, and polyphenol oxidase activities. Additionally, BEO reduced NAD/NADH levels, while increasing NADP/NADPH levels. In summary, BEO fumigation can enhance the antioxidant capacity, mitigate membrane peroxidation, modulate energy and respiration metabolism, thereby preserving the storage quality of blueberries.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113418"},"PeriodicalIF":6.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative N-glycomics and N-glycoproteomics landscape reveals up-regulation of complex-type N-glycosylation in the tolerance of peach fruit to chilling injury","authors":"Huiwen Wang ,&nbsp;Tonghao Cui ,&nbsp;Jiaqi Wang ,&nbsp;Ruohan Ou ,&nbsp;Yilong Liu ,&nbsp;Bo Zhang ,&nbsp;Changjie Xu ,&nbsp;Changqing Zhu ,&nbsp;Miaojin Chen ,&nbsp;Yanna Shi ,&nbsp;Yuanjiang Pan ,&nbsp;Kunsong Chen ,&nbsp;Xiaoyong Zhao ,&nbsp;Xian Li","doi":"10.1016/j.postharvbio.2025.113433","DOIUrl":"10.1016/j.postharvbio.2025.113433","url":null,"abstract":"<div><div>Post-harvest chilling injury (CI) is a severe problem that causes great economic losses. The peach is an important fruit consumed worldwide but susceptible to CI under post-harvest storage, and thus serves as the typical model for CI research. Protein N-glycosylation plays a vital role under abiotic stress in plants. Here, a CI-associated up-regulation of N-glycosylation was observed in peach fruit stored at 5 ℃. An integrative N-glycomics and N-glycoproteomics analysis by reactive matrix-assisted laser desorption ionization time-of-flight mass spectrometry and ¹⁸O-labeled tagging with nano-liquid chromatography-electrospray ionization tandem mass spectrometry, respectively, was developed to provide first insights into N-glycosylation remodeling for fruit tolerance to CI. On one side, 27 N-glycans were identified by N-glycomics analysis and seven complex-type N-glycans were substantially up-regulated in CI group. On the other side, N-glycoproteomics analysis identified 1002 N-glycosites on 732 N-glycoproteins. Differential and gene set analyses revealed 88 common N-glycoproteins, based on which three KEGG pathways including metabolic pathways, amino sugar &amp; nucleotide sugar metabolism, and protein processing in endoplasmic reticulum (ER) were highly enriched. Among N-glycoproteins in top three enriched KEGG pathways, 29 N-glycosites were anchored and nearly 80 % of them were up-regulated in CI group. The corresponding 22 hub N-glycoproteins were primarily involved in CI-related biological processes such as phytohormone signaling, redox homeostasis, biotic stress, ER quality control, cell wall metabolism, membrane lipid metabolism, and soluble sugar metabolism. Taken together, up-regulation of N-glycosylation, particularly complex-type N-glycosylation, as cellular responses to enhance chilling resistance may provide novel molecular insights and thus potential post-harvest solutions.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113433"},"PeriodicalIF":6.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electron beam irradiation maintains postharvest quality of Actinidia arguta by regulating the cell wall, starch degradation, and antioxidant capacity","authors":"Qi Zheng ,&nbsp;Wenhui Tian ,&nbsp;Shanshan Wang ,&nbsp;Zhijun Chen ,&nbsp;Haihong Wang ,&nbsp;Ling Yue ,&nbsp;Weiqiang Yan ,&nbsp;Wenyuan Qi ,&nbsp;Ci Zhang ,&nbsp;Xiaoyan Xu ,&nbsp;Qiulian Kong","doi":"10.1016/j.postharvbio.2025.113442","DOIUrl":"10.1016/j.postharvbio.2025.113442","url":null,"abstract":"<div><div>Rapid postharvest softening, a major issue with <em>Actinidia arguta</em>, leads to a decline in quality and in commercial value. To maximally preserve fruit quality, the effects of different doses of electron beam irradiation (0.4 kGy and 1 kGy) on fruit softening and the antioxidant capacity of <em>A. arguta</em> after 30 days of storage were investigated. The results indicated that, compared to unirradiated fruits, <em>A. arguta</em> fruit subjected to electron beam irradiation maintained high levels of firmness, total phenols, flavonoids, protopectin, starch, and sucrose, and exhibited low levels of malondialdehyde and reducing sugars. Fruit receiving 1 kGy irradiation exhibited the highest firmness; however, a significant reduction in firmness was observed immediately after irradiation (day 0). An irradiation dose of 0.4 kGy did not decrease fruit firmness, and there were no significant differences in the contents of protopectin, starch, sucrose, total phenols, and flavonoids compared to fruits treated with 1 kGy irradiation. A transcriptomic analysis revealed that electron beam irradiation significantly downregulated the most genes associated with the cell wall and starch degradation, and significantly upregulated some genes related to antioxidant activity. Furthermore, there was a positive correlation between the upregulated and downregulated gene expression levels and the radiation dose. The unigenes obtained from the transcriptomic analysis were validated through integrated RT-qPCR, proteomic, and transcriptomic correlation analyses. Overall, these findings indicated that electron beam irradiation is an effective method for delaying the softening of <em>A. arguta</em>.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113442"},"PeriodicalIF":6.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between plant hormones in response to Botrytis cinerea infection in cut flowers with differential tissue sensitivity to ethylene","authors":"Suong Tuyet Thi Ha,&nbsp;Ji Yeong Ham,&nbsp;Yong-Tae Kim,&nbsp;Byung-Chun In","doi":"10.1016/j.postharvbio.2025.113441","DOIUrl":"10.1016/j.postharvbio.2025.113441","url":null,"abstract":"<div><div><em>Botrytis cinerea</em> (<em>B. cinerea</em>), a major postharvest disease-causing fungus, causes considerable ornamental and economic losses in cut flowers, but the mechanisms by which phytohormones modulate <em>B. cinerea</em> resistance in cut flowers remain unclear. Here, we explored the role of plant hormones including ethylene (ETH), jasmonic acid (JA), and salicylic acid (SA), in the response to <em>B. cinerea</em> infection in rose cultivars with different ETH sensitivities. The results showed that ETH-highly sensitive (EthHS) cultivars exhibited faster disease progression and more severe symptoms of gray mold disease (GMD) symptoms than ETH-low sensitive (EthLS) cultivars when exposed to <em>B. cinerea</em> and ETH. <em>B. cinerea</em> infection significantly altered the expression patterns of ETH, JA, and SA biosynthesis and the signaling genes in rose petals. Notably, the activation of ETH- and SA-related genes by the GMD infection was closely related to the ETH sensitivity of the petal tissues, while JA signaling functioned independently. ETH and SA treatments increased the GMD susceptibility by upregulating <em>B. cinerea</em> histidine kinase receptors and pathogenicity-related genes, thereby accelerating tissue senescence in EthHS roses with or without <em>B. cinerea</em> infection. Contrastingly, the EthLS flowers treated with ETH and SA showed elevated GMD symptoms only when inoculated with fungi. Methyl JA (MeJA) treatment reduced the GMD severity and downregulated the transcription of <em>B. cinerea</em>-related genes, regardless of the tissue sensitivity to ETH. A working model was established to illustrate the antagonistic roles of ETH and SA against JA in regulating <em>B. cinerea</em> resistance in cut roses. These insights into hormone crosstalk provide potential strategies for enhancing <em>B. cinerea</em> resistance in cut rose flowers.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113441"},"PeriodicalIF":6.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diacetyl delays postharvest leaf yellowing of pak choi (Brassica rapa subsp. Chinensis) by inhibiting ABA synthesis","authors":"Xiaotong Li ,&nbsp;Hongliang Li ,&nbsp;Qingguo Wang ,&nbsp;Song Zhang","doi":"10.1016/j.postharvbio.2025.113446","DOIUrl":"10.1016/j.postharvbio.2025.113446","url":null,"abstract":"<div><div>Pak choi (<em>Brassica rapa</em> subsp. <em>Chinensis</em>) is a key vegetable in daily life, however, yellowing of its leaves poses a significant challenge during postharvest storage. Diacetyl, a naturally occurring substance, was found to delay postharvest leaf yellowing of pak choi in this research. Additionally, the regulatory mechanism associated with diacetyl-mediated inhibition of leaf yellowing in pak choi was elucidated. We observed that diacetyl (7 μL L^–1) delayed the leaf yellowing and suppressed chlorophyll degradation related enzyme activities. Various chlorophyll degradation related genes were also repressed following diacetyl treatment. Transcriptome analysis identified numerous differentially expressed genes (DEGs) in diacetyl-treated group compared to control group. Analysis of DEGs revealed that diacetyl significantly regulated genes involved in abscisic acid (ABA) biosynthesis and the ABA signaling pathway. Furthermore, diacetyl treatment inhibited ABA accumulation in pak choi. Importantly, a NAC (NAM, ATAF and CUC) transcription factor (TF), BcNAC100, which was repressed by diacetyl, was associated with leaf yellowing in pak choi. BcNAC100 was localized in nuclear and had high homology to ANAC100. Significantly, BcNAC100 can bind to the promoters of <em>BcNCED3</em> (nine-cis-epoxycarotenoid dioxygenase 3) and <em>BcZEP</em> (zeaxanthin epoxidase) genes, positively regulating their expression to enhance ABA synthesis. These results demonstrated that diacetyl repressed BcNAC100 expression, reducing ABA accumulation and delaying leaf yellowing in pak choi. Furthermore, these findings revealed a novel molecular regulatory pathway in regulating leaf yellowing of pak choi and identified diacetyl as a potential vegetable preservation agent.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113446"},"PeriodicalIF":6.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing fruit quality through unraveling the molecular and physiological basis and preventing cracking in jujube (Ziziphus jujuba Mill.)","authors":"Hong-Xiang Zhou ,&nbsp;Run-Zhe Wang ,&nbsp;Wen-Ting Luo ,&nbsp;Yang Liang ,&nbsp;Shi-Yu Liu ,&nbsp;Juan Chen ,&nbsp;Ji-Qing Peng ,&nbsp;Yan-Xia Fan ,&nbsp;Shu-Zhan Li ,&nbsp;Chuan Guo ,&nbsp;Zi-Qiang Zhang ,&nbsp;Feng-Xia Shao ,&nbsp;Song Sheng ,&nbsp;Sen Wang","doi":"10.1016/j.postharvbio.2025.113417","DOIUrl":"10.1016/j.postharvbio.2025.113417","url":null,"abstract":"<div><div>Jujube (<em>Ziziphus jujuba</em> Mill.), a ‘woody grain’ tree with medicinal and dietary uses, contains various vitamins, cyclic adenosine monophosphate, and essential trace elements. This study found that precipitation and temperature significantly contributed to jujube fruit cracking, with high temperatures and rainfall damaging the fruit epidermis and leading to swelling and cracking. The fruit bottom and surface areas were most prone to cracking. Differentially expressed gene analysis revealed 1957 DEGs between cracking-resistant and susceptible jujube cultivars, linked to cell wall structure, growth processes, and auxin transport. This suggests resistance may be due to modified cell wall properties, controlled cell growth, and regulated auxin transport. Applying amino acid solution at a concentration of 143 mg/L increased epidermal thickness and reduced the cracking rate by 37.5 %, showing significant anti-cracking effects.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113417"},"PeriodicalIF":6.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of bruising in pear with varying bruising degrees and formation times by using SIRI technique combining with texture feature-based LS-SVM and ResNet-18-based CNN model","authors":"Jiangbo Li ,&nbsp;Junyi Zhang ,&nbsp;Mengwen Mei ,&nbsp;Zhihua Diao ,&nbsp;Xuetong Li ,&nbsp;Ruiyao Shi ,&nbsp;Zhonglei Cai","doi":"10.1016/j.postharvbio.2025.113434","DOIUrl":"10.1016/j.postharvbio.2025.113434","url":null,"abstract":"<div><div>Bruising negatively affects the appearance and sensory quality of fruit, weakening their commercial value. Optical non-destructive testing of bruised fruit is an important research topic. However, the degree and formation time of bruising have a significant impact on detection accuracy, which poses a challenge for accurately detecting bruised fruit. This study attempted to use structured-illumination reflectance imaging (SIRI) to detect bruised pears with different degrees and formation times. Structured-illumination images of pears with three bruising levels (S1, S2 and S3) and five bruising formation times (0, 6, 24, 48 and 72 hours) were collected. Two machine learning models including texture feature-based least squares support vector machine (LS-SVM) and ResNet-18-based convolutional neural network (CNN) were established using different input images (DC, AC, RT, DC-AC and DC-AC-RT). Study indicated that AC and RT images have depth resolution capability, achieving good results for detecting pear bruises within 24 hours combining with two types of models, showing that the powerful detection capability of SIRI technology for early bruising. Compared to LS-SVM model, the ResNet-18-based CNN model obtained better detection performance, and its detection accuracy was not significantly affected by the bruising degree and formation time. For all samples, ResNet-18-based CNN model, combined with AC and RT images, achieved an overall detection accuracy of 92.5 % and 94.3 %, respectively. This study provided a valuable solution for accurate identification of bruised pears with varying bruising degrees and times.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113434"},"PeriodicalIF":6.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis in postharvest storability, quality attributes, antioxidant activity, and disease defense systems in ‘Fuyan’ and ‘Dongbi’ longans during storage","authors":"Yueying Sang ,&nbsp;Lijuan Lin ,&nbsp;Hongyi Wen ,&nbsp;Yixiong Lin ,&nbsp;Mengshi Lin ,&nbsp;Yihui Chen ,&nbsp;Hui Wang ,&nbsp;Zhongqi Fan ,&nbsp;Yifen Lin ,&nbsp;Hetong Lin","doi":"10.1016/j.postharvbio.2025.113422","DOIUrl":"10.1016/j.postharvbio.2025.113422","url":null,"abstract":"<div><div>This research aimed to compare the fruit storability and quality differences between the ‘Fuyan’ and ‘Dongbi’ varieties of longan (<em>Dimocarpus longan</em> Lour.) by assaying their antioxidant system and disease defense systems. The results showed that, compared to ‘Fuyan’ fruit, during storage, ‘Dongbi’ fruit exhibited superior storability and appearance, characterized by lower levels of fruit disease, pulp breakdown, pericarp browning, and weight loss, alongside higher pericarp color, brightness, pigments, pulp nutrient content, and commercial acceptability of fruit. Additionally, ‘Dongbi’ longans exhibited lower ROS accumulation and MDA level, higher levels of antioxidants (AsA, GSH), antioxidant enzymes (SOD, CAT, APX), reducing power, and DPPH• scavenging ability. Besides, ‘Dongbi’ longans retained higher levels of secondary metabolites (lignin, total phenolics, flavonoid), PRMEs (PAL, C4H, 4-CL, CAD, POD), and PRs (CHI, GLU). Therefore, the enhanced storage stability of ‘Dongbi’ longans may be attributed to the synergistic effects of an improved antioxidant system, phenylpropanoid pathway, and PRs.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113422"},"PeriodicalIF":6.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing preharvest and packinghouse data in combination with storage trials to develop an intelligent logistic management system for 'Orri' mandarins","authors":"Abiola Owoyemi ,&nbsp;Moria Balaklav ,&nbsp;Ron Porat ,&nbsp;Amnon Lichter ,&nbsp;Aviv Goren ,&nbsp;Noam Koenigstein ,&nbsp;Yael Salzer","doi":"10.1016/j.postharvbio.2025.113452","DOIUrl":"10.1016/j.postharvbio.2025.113452","url":null,"abstract":"<div><div>'Orri' mandarin is the main citrus variety grown and exported from Israel. In the current study, we assessed the potential of utilizing large-scale preharvest and commercial packinghouse data to predict the postharvest storage performances of different sets of 'Orri' mandarins. For that purpose, we examined during 3 years (2022–2024), the postharvest performances of between 79 and 93 sets of 'Orri' mandarins per year after 2, 4 and 8 weeks storage at 5 °C and one-week at shelf life at 22 °C. The observed preharvest data consisted climatic features (amounts of rain and warm days) and horticultural features (harvest time, tree age, yield, and soil type), and the observed packinghouse data consisted ripening features (total soluble solids, acidity and juice contents) and packing line quality evaluation features (occurrences of various defects and packaging rates). Consolidating the observed preharvest and packinghouse data with the postharvest storage data using machine learning algorithms, allowed the prediction of decay percentages by an R² of 0.60 and RMSE of 10.56 %, and of fruit quality acceptance score by an R² of 0.74 and RMSE of 0.57 on a quality scale from 1 (very bad) to 5 (excellent). The development of fruit quality prediction models based on preharvest and packinghouse data can assist in the future implementation of intelligent logistic management system that will allow to direct specific fruit sets to different market destinations based on their predicted shelf life's, thus assuring that only high-quality produce will arrive at the recipient markets.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113452"},"PeriodicalIF":6.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of SlIMP3 regulates sugar metabolism, antioxidant activity, and fruit softening, leading to extended shelf life of tomato fruits","authors":"Qiqi Yang ,&nbsp;Xianzhe Zheng ,&nbsp;Xu Fang ,&nbsp;Qiongdan Zhang ,&nbsp;Jie Deng ,&nbsp;Jiarui Han ,&nbsp;Chan Xu ,&nbsp;Qiaoli Xie ,&nbsp;Wei Deng","doi":"10.1016/j.postharvbio.2025.113429","DOIUrl":"10.1016/j.postharvbio.2025.113429","url":null,"abstract":"<div><div>During the postharvest storage of tomato fruits, they often shrink or even rot due to water loss or infection by pathogenic bacteria, leading to significant economic losses in the tomato fruit industry. Inositol monophosphatase (IMP) is involved in inositol synthesis, which serves as a precursor for the synthesis of cell wall polysaccharides and is also involved in ascorbic acid synthesis. Overexpression of <em>SlIMP3</em> in tomato has been shown to delay fruit softening, but the mechanism by which <em>SlIMP3</em> regulates fruit softening remains unclear. In this study, the transcriptome and metabolome of wild-type tomato fruit (WT) and <em>SlIMP3</em> overexpressing tomato fruit were thoroughly analyzed at 0, 15 and 26 days of storage. A total of 83 differential metabolites were identified, and more than 2000 genes exhibited changes during storage and in <em>SlIMP3</em>-OE tomato fruits. KEGG pathway enrichment analysis revealed that the differential genes were mainly related to metabolic pathways and the biosynthesis of secondary metabolites. Metabolome analysis showed that various sugars (lythreon, glucose, cellobiose, allulose, etc.) and antioxidants (melatonin, 3,4-dihydroxymandelic acid, dihydroquercetin, salicylic acid) underwent significant changes during storage. Significantly, most genes associated with cell wall degradation (<em>SlEXP1</em>, <em>SlPL</em>, <em>SlPG2</em>, <em>SlTBG4</em>, <em>SlXYL1</em>, <em>SlXTH5</em>) were downregulated in <em>SlIMP3</em>-OE compared to the wild type. Two-omics analysis indicated that overexpression of <em>SlIMP3</em> regulated the accumulation of many carbohydrates and antioxidants and the expression of related anabolic genes during storage of tomato fruits. In conclusion, overexpression of <em>SlIMP3</em> affects tomato fruit softening through the modulation of carbohydrate and antioxidant metabolism, concurrently regulating the expression of genes associated with cell wall metabolism. This study provides new theoretical insights and potential methods for improving fruit quality and reducing postharvest loss.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113429"},"PeriodicalIF":6.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}