Postharvest Biology and Technology最新文献

{"title":"The use of Trichoderma species for the biocontrol of postharvest fungal decay in fruits and vegetables: Challenges and opportunities","authors":"Xiaojiao Li ,&nbsp;Qinhong Liao ,&nbsp;Shixian Zeng ,&nbsp;Yong Wang ,&nbsp;Jia Liu","doi":"10.1016/j.postharvbio.2024.113236","DOIUrl":"10.1016/j.postharvbio.2024.113236","url":null,"abstract":"<div><div>Ensuring the stability of the global supply of fruits and vegetables and maintaining the quality and nutritional value of fruits and vegetables during storage and marketing are critical problems that need to be continually addressed. However, postharvest losses due to decay and senescence pose a major threat to the supply of high-quality fruits and vegetables. In fact, postharvest losses caused by decay pathogens can account for 20–25 % of postharvest losses. In recent years, new methods to control postharvest diseases of fruits and vegetables have been explored that are considered safer and more ecofriendly than the use of synthetic, chemical fungicides. In this regard, the use of <em>Trichoderma</em> species as biocontrol agents of both preharvest and postharvest diseases has been the subject of considerable research. Their use for the prevention of postharvest diseases is especially noteworthy as <em>Trichoderma</em> has been shown to be non-toxic and non-harmful to humans and the environment. In this review we provide an overview of the advances that have been made in the use of <em>Trichoderma</em> isolates for controlling postharvest diseases of fruits and vegetables. The review provides information on the identification of <em>Trichoderma</em> species with biocontrol potential, their mode of action, and the challenges and opportunities that exist for the commercial application of <em>Trichoderma</em> in the postharvest environment. Future research topics that need to be addressed on the use of <em>Trichoderma</em> are also presented.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113236"},"PeriodicalIF":6.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular polygalacturonase, β-1,4-glucanase and β-xylosidase in Geotrichum citri-aurrantii positively progressed the sour rot incidence in satsuma mandarin fruit","authors":"Haowen Ai,&nbsp;Yonghua Zhang,&nbsp;Okwong Oketch Reymick,&nbsp;Xuemeng Shen,&nbsp;Wen Liu,&nbsp;Nengguo Tao,&nbsp;Lu Li","doi":"10.1016/j.postharvbio.2024.113233","DOIUrl":"10.1016/j.postharvbio.2024.113233","url":null,"abstract":"<div><div>Gaining an understanding of the characteristics that contribute to the infectiousness of pathogens is essential for devising new strategies to control fungal infection. This study aims to identify and clarify the role of cell wall degrading enzymes (CWDEs) secreted by the <em>Geotrichum citri-aurantii</em> pathogen in the development of sour rot disease in Satsuma mandarin fruit. Results from <em>in vivo</em> experiments showed that CWDEs were secreted during arthrospore germination. Genomic sequencing and liquid chromatography-tandem mass spectrometry results unveiled the presence of polygalacturonase (PG), β-1,4-glucanase, α-glucosidase, xyloglucan:xyloglucosyl transferase, and β-xylosidase. Enzyme activity and gene expression analyses exposed the indispensable roles of PG and β-1,4-glucanase in the initial phase, and β-xylosidase during the acute phase of infection. After infection, we observed that the quantities of cellulose, pectin, and hemicellulose declined continuously, while the soluble pectin content rose steadily. Molecular docking analysis demonstrated that the diversity of CWDE binding to substrates complicated the <em>G. citri-aurantii</em> infection process. Furthermore, we determined the importance of asparagine and aspartic acid in PG-substrate interaction and glycine in β-xylosidase-ligand interaction. Taken together, our findings suggest that PG is a crucial target for blocking the development of sour rot in citrus fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113233"},"PeriodicalIF":6.4,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pickering emulsions stabilized by chitosan nanoparticles and loaded with clove essential oil as a green biopesticide: A case study on controlling fungal growth in oranges","authors":"Maryam Mottaki ,&nbsp;Seid Mahdi Jafari ,&nbsp;Seyyed Morteza Hosseini ,&nbsp;Alireza Sadeghi ,&nbsp;Fatemeh Heydari-Delfard","doi":"10.1016/j.postharvbio.2024.113234","DOIUrl":"10.1016/j.postharvbio.2024.113234","url":null,"abstract":"<div><div>The purpose of this research was to optimize the production of Pickering emulsions (PEs) stabilized by chitosan nanoparticles (CS-NPs) and containing clove essential oil (CEO) as a biopesticide against the fungal growth (<em>Penicillium digitatum</em>) in oranges. First, CS-NPs were synthesized and characterized to prepare PEs. The produced CS-NPs had an average size &lt; 100 nm, contact angle &lt; 90° and a positive zeta potential; CS-NPs were hydrophilic, which showed their ability to stabilize oil-in-water PEs. Second, PEs of CEO in water stabilized by CS-NPs were prepared at different levels of nanoparticles, CEO content, and homogenizer speeds; FTIR spectrum, morphology, size and stability of these PEs during storage were studied. The optimal conditions for the production of PEs included 0.055 % CS-NPs, 1 % CEO and 25,000 rpm homogenization. This PE had an encapsulation efficiency of 98 % and a gradual and appropriate release during the storage time. In the third phase, the antifungal activity of different concentrations of PEs compared with Xedamix (a commercial fungicide) was investigated with the aim of increasing the shelf life of oranges after harvesting. According to <em>in vitro</em> antifungal activity, minimum fungicidal concentration of the produced PEs against <em>P. digitatum</em> was equal to 2 %. Moreover, there was no significant difference between <em>in situ</em> inhibitory effects of PEs and Xedamix on the target fungus in spiked oranges.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113234"},"PeriodicalIF":6.4,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Storage temperature affects metabolism of sweet corn","authors":"Shiyu Liu ,&nbsp;Xinyuan Zhou ,&nbsp;Yunxiang Wang ,&nbsp;Qing Wang ,&nbsp;Lili Ma ,&nbsp;Cai’e Wu ,&nbsp;Ronghuan Wang ,&nbsp;Yaxing Shi ,&nbsp;Christopher B. Watkins ,&nbsp;Jinhua Zuo ,&nbsp;Yanyan Zheng","doi":"10.1016/j.postharvbio.2024.113232","DOIUrl":"10.1016/j.postharvbio.2024.113232","url":null,"abstract":"<div><div>Temperature is one of the critical factors affecting the postharvest storage quality of sweet corn, but effects of storage temperatures have not been elucidated at the molecular level. In this study, the effects of storage at 0 °C, 4 °C and 20 °C, on quality and associated effects on the transcriptome and metabolome were investigated. Compared with storage at 20 °C, cobs stored at 0 °C and 4 °C had up-regulated expression of genes associated with cell wall disassembly (<em>PG, PE, BMY, β-Gal</em>) and down-regulated expression of genes involved in lignin synthesis, the up-regulation of <em>SPP, SPS</em> and <em>SUS</em> involved in sucrose synthesis, and the down-regulation of <em>IN, FK, HXK</em> and <em>PFK</em> involved in sucrose catabolism. Most of the genes involved in ethylene biosynthesis and signaling were inhibited at 0 °C, except for <em>ERFs</em>, while abscisic acid synthesis and signal transduction were most active at this temperature. Near-freezing temperature also maintains the integrity of the cell membrane by inhibiting the expression of <em>PLD, PLC</em> and <em>LOX</em>, which encode membrane lipid-degrading enzymes. Metabolomics and combined analysis showed that the contents of lysophospholipid, glutathione and L-ascorbic acid in sweet corn increased at low temperatures. These results provide insight into the effects of low temperatures on sweet corn quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113232"},"PeriodicalIF":6.4,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ursolic acid, a natural endogenous compound, inhibits browning in fresh-cut apples","authors":"Song Zhang ,&nbsp;Shuwen Wang ,&nbsp;Yu Li,&nbsp;Jinjie Wang,&nbsp;Jingying Shi,&nbsp;Yong Peng,&nbsp;Pei Liu","doi":"10.1016/j.postharvbio.2024.113228","DOIUrl":"10.1016/j.postharvbio.2024.113228","url":null,"abstract":"<div><div>Enzymatic browning occurs rapidly in fresh-cut apples and can lead to economic losses. Although various methods have been developed to prevent this browning, physical methods are costly, and chemical methods are controversial, suggesting the potential usefulness of an endogenous natural browning inhibitor. In this study, the effects of ursolic acid (UA), a natural endogenous compound, on apple browning were investigated. It was found that UA inhibited the activity of polyphenol oxidase through Cu²⁺ chelation. Molecular docking indicated that the inhibition was reversible and mixed-type. Moreover, UA treatment promoted the scavenging of reactive oxygen species in fresh-cut apple and reduced the proliferation of microorganisms on the apple surface. Similar effects were observed on the browning of chestnut, eggplant, potatoes, and pears. In conclusion, the findings demonstrate the efficacy of UA on inhibiting browning in fresh-cut apple, together with an analysis of the mechanism involved.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113228"},"PeriodicalIF":6.4,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of terpene synthase genes accounting for volatile terpene emissions in the flower of Paeonia lactiflora","authors":"Qian Zhao ,&nbsp;Yuqing Li ,&nbsp;Lina Gu ,&nbsp;Shuting Li ,&nbsp;Di He ,&nbsp;Shanrong Dong ,&nbsp;Qingyu Zhang ,&nbsp;Jianrang Luo ,&nbsp;Yanlong Zhang","doi":"10.1016/j.postharvbio.2024.113231","DOIUrl":"10.1016/j.postharvbio.2024.113231","url":null,"abstract":"<div><div>Emergence of floral scents marked a significant milestone in biological development of plant, letting out olfactory signals that enable them to induce pollinators. In this research, molecular and metabolomics methodologies were used to explore terpene synthase (TPS) genes, which are responsible for synthesis of floral fragrance in <em>Paeonia lactiflora</em> ‘Wu Hua Long Yu’. Analyses on transcriptome data identified 11 <em>PlTPS</em> genes, among them 7 genes were isolated, and the functions of 5 genes were confirmed. <em>PlTPS1</em> was identified as a single-product enzyme; <em>PlTPS4</em>, <em>8</em> and <em>9</em> were identified as bifunctional enzymes, capable of recognizing farnesyl pyrophosphate and geranyl pyrophosphate; <em>PlTPS6</em> generated multiple products by recognizing farnesyl pyrophosphate. Enzymatic products of these PlTPS proteins closely matched the volatile terpenes emitted by flowers, revealing the relation between <em>PlTPS</em> genes and the release of such volatile compounds. Functions of five <em>PlTPS</em> genes in herbaceous peony were further elucidated through virus-induced gene silencing experiments, as well as overexpression in tobacco. <em>PlTPS1</em> catalyzes geraniol production, while <em>PlTPS4</em> catalyzes linalool. <em>PlTPS6</em> is responsible for producing two sesquiterpenes, caryophyllene and α-humulene. <em>PlTPS8</em>, with the most varied products, mainly catalyzes the production of α-pinene, linalool, and germacrene D. Based on these findings, graphical models summarizing the biosynthesis of floral volatile terpenes in herbaceous peony have been proposed. The characterization of <em>PlTPS</em> genes has elucidated their specific roles in the synthesis of herbaceous peony fragrance, opening up possibilities for introducing or enhancing floral scents in herbaceous peony and other plants.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113231"},"PeriodicalIF":6.4,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a unified framework of low-rank approximation and deep neural networks for predicting the spatial variability of SSC in `Spania' watermelons using vis/NIR hyperspectral imaging","authors":"Jobin Francis ,&nbsp;Sony George ,&nbsp;Binu M. Devassy ,&nbsp;Sudhish N. George","doi":"10.1016/j.postharvbio.2024.113222","DOIUrl":"10.1016/j.postharvbio.2024.113222","url":null,"abstract":"<div><div>Soluble Solids Content (SSC) is an important quality attribute that represents the internal quality of fruits. Visible and near-infrared (Vis/NIR) combined with chemometric algorithms are now popular methods for non-invasive measurement and visualization of SSC. However, in fruits with a thick rind and a large flesh volume, such as watermelon, SSC is not evenly distributed across the fruit. The variability in SSC across the fruit flesh may have an adverse effect on the accuracy of quality analysis algorithms. Thus, this paper presents an accurate and efficient approach for predicting the spatial variation of SSC in watermelons using a combined framework of low-rank approximation and deep neural networks. Watermelon ‘Spania’ was selected for this study, and hyperspectral images of each watermelon were taken from various views, including the top, bottom, and two lateral views. The low-rank property is employed as a constraint in this approach to eliminate the unwanted variations in the spectral data. The low-rank component of the spectral data, free of unwanted variations, is then fed into a fully connected neural network (FNN) for the prediction of watermelon SSC values. The proposed approach obtained optimal performance in calibration and prediction with <span><math><mrow><msubsup><mrow><mspace></mspace><mtext>R</mtext><mspace></mspace></mrow><mrow><mi>C</mi></mrow><mrow><mn>2</mn></mrow></msubsup><mo>=</mo><mn>0.982</mn></mrow></math></span>, RMSEP = 0.132, and <span><math><mrow><msubsup><mrow><mspace></mspace><mtext>R</mtext><mspace></mspace></mrow><mrow><mi>P</mi></mrow><mrow><mn>2</mn></mrow></msubsup><mo>=</mo><mn>0.945</mn></mrow></math></span>, RMSEP = 0.195 respectively. Further, the prediction outcomes of the proposed method were compared with state-of-the-art such as Partial Least Square Regression (PLSR), Support Vector Regression (SVR), Multiple Linear Regression (MLR), Decision Tree (DT), and Random Forest (RF). The overall results showed that combining HSI data with a low-rank and deep neural network framework is an efficient method for accurately predicting SSC in watermelons as well as correctly predicting spatial variability of SSC in individual fruits.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113222"},"PeriodicalIF":6.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and metabolic insights into the mechanism of exogenous methyl jasmonate in enhancing the postharvest resistance of kiwifruit to Botrytis cinerea","authors":"Jiaqi Yang ,&nbsp;Yijia Ma ,&nbsp;Tianjing Zeng,&nbsp;Zhexin Li,&nbsp;Yuan Sui,&nbsp;Wenlin Zhang,&nbsp;Hongpan Zhong,&nbsp;Xu Wang","doi":"10.1016/j.postharvbio.2024.113226","DOIUrl":"10.1016/j.postharvbio.2024.113226","url":null,"abstract":"<div><div>Gray mold, caused by <em>Botrytis cinerea</em> infection, significantly impacts postharvest kiwifruit, leading to spoilage and food safety issues. Meanwhile, methyl jasmonate (MeJA) induces defense responses in plants. This study aimed to identify the optimal MeJA concentration to induce disease resistance in kiwifruits. Notably, various plant defense enzymes were detected in MeJA-treated kiwifruit. Moreover, 0.01 mol/L MeJA was found to enhance <em>B. cinerea</em> resistance in kiwifruit by increasing antioxidant enzyme activity. Widely targeted metabolomics analysis revealed 62–64 differentially expressed metabolites (DEMs) between the different treatment groups. Additionally, RNA-seq analysis revealed 930–2900 differentially expressed genes (DEGs), between these groups. Subsequently, combined DEG and DEM analysis highlighted phenylpropanoid biosynthesis and plant hormone signaling as key transduction pathway associated with MeJA-induced resistance. Overall, these findings identified the mechanism of MeJA-induced resistance in kiwifruit, providing a theoretical basis for the safe and effective control of postharvest diseases in kiwifruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113226"},"PeriodicalIF":6.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine localization and functional differentiation of exocarp cell clusters during fruit senescence revealed by single-cell and spatial transcriptomics","authors":"Jingyu Jia ,&nbsp;Enyan Chen ,&nbsp;Yajing Tian ,&nbsp;Jiahao Shen ,&nbsp;Xinyue Pang ,&nbsp;Xin Li","doi":"10.1016/j.postharvbio.2024.113223","DOIUrl":"10.1016/j.postharvbio.2024.113223","url":null,"abstract":"<div><div>The differentiation trajectories of 13 cell clusters in <em>H. undatus</em> during senescence have been revealed by single-cell transcriptome (scRNA-seq) analysis, and the five cell clusters in the exocarp have been found to induce resistance mainly. However, the precise localization and functional differentiation of these five cell clusters remain unclear. In this study, phenotypic changes in the exocarp were recorded, and the ROS production and flavonoid biosynthesis in the exocarp were elucidated. The trajectories of the cell clusters involved in exocarp function were further revealed in scRNA-seq profiles. Through integrated analysis of scRNA-seq and spatial RNA-seq data at resolutions of 0.5, 0.6, and 1.5, using algorithms SingleR, SciBet, CARD, and RCTD, the fine localization of these clusters from the outer to inner layers was achieved. RNA-FISH was used to validate the specific expression of marker genes <em>HuSP2</em> and <em>HuCEL2</em> in different locations of the exocarp. Finally, the differentiation trajectories of the five cell clusters were analyzed by selecting highly correlated cells with specific functions for each cluster. The core roles of <em>HuPRP</em> and <em>HuCRRSP55</em> in the gene regulatory networks of exocarp clusters were revealed. A hypothesis that the five clusters in the exocarp participate in the induction of resistance was proposed.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113223"},"PeriodicalIF":6.4,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S092552142400468X/pdfft?md5=6510d0cfd8ff1e5594ec82e61fdcac1e&pid=1-s2.0-S092552142400468X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CpAGL18 is involved in the cross-talk of ethylene and auxin signaling in the regulation of fruit ripening in papaya","authors":"Jiahui Cai ,&nbsp;Ziling Wu ,&nbsp;Wang Zheng ,&nbsp;Qiunan Zhu ,&nbsp;Zunyang Song ,&nbsp;Weixin Chen ,&nbsp;Xueping Li ,&nbsp;Xiaoyang Zhu","doi":"10.1016/j.postharvbio.2024.113221","DOIUrl":"10.1016/j.postharvbio.2024.113221","url":null,"abstract":"","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113221"},"PeriodicalIF":6.4,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142651001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}