Postharvest Biology and Technology最新文献

{"title":"Mediating effect of auxin and PsYUC10B on petal abscission and flower longevity in tree peony exposed to high temperature","authors":"Chunying Liu ,&nbsp;Yu Du ,&nbsp;Jinghan Zhang ,&nbsp;Ziqi Liu,&nbsp;Jia Gu,&nbsp;Qing Liu,&nbsp;Yanchao Yuan,&nbsp;Yuxi Zhang,&nbsp;Shupeng Gai","doi":"10.1016/j.postharvbio.2025.113907","DOIUrl":"10.1016/j.postharvbio.2025.113907","url":null,"abstract":"<div><div>Tree peony, valued for its ornamental and medicinal properties, is a renowned ornamental plant resource in China. However, its natural florescence is short, and high temperature often occur during this time, which can further reduce floral longevity. The short florescence significantly limits the advancement of the tree peony industry. Therefore, elucidating the regulatory mechanisms underlying flower senescence and petal abscission is crucial for developing strategies to extend floral longevity. This study investigated the regulatory roles of auxin in flower senescence and petal abscission, focusing specifically on the <em>PsYUC10B</em> gene in tree peony. The results revealed that the application of auxin delayed flower senescence and petal abscission. Conversely, high temperatures disrupted the auxin gradient across abscission zone and accelerated its formation. The differentially expressed <em>PsYUC10B</em> gene, which encodes a key enzyme in auxin biosynthesis, was obtained and identified in tree peony based on transcriptomic and proteomic data. Overexpression of <em>PsYUC10B</em> delayed flower senescence and petal abscission, while silencing <em>PsYUC10B</em> reduced floral lifespan. Additionally, silencing <em>PsYUC10B</em> resulted in a significant upregulation of genes encoding endoglucanase, polygalacturonase, pectin esterase, and hexokinase—enzymes involved in pectin and cellulose degradation—and a downregulation of chalcone synthase and L-ascorbate oxidase genes. Furthermore, heterologous expression of <em>PsYUC10B</em> in <em>Arabidopsis</em> confirmed its role in regulating petal abscission. These findings provide novel insights into the auxin pathway’s involvement in petal abscission and highlight <em>PsYUC10B</em> as a potential target for enhancing floral longevity in tree peony and other ornamental plants.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"231 ","pages":"Article 113907"},"PeriodicalIF":6.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms underlying the regulation of anthocyanin metabolism in postharvest blueberries by microcapsule-containing thymol coatings","authors":"Xi Wei ,&nbsp;Jing Xie ,&nbsp;Tingting Tang ,&nbsp;Shanshan Lei ,&nbsp;Weirui Fang ,&nbsp;Yuxin Liu ,&nbsp;Jiayi Zheng ,&nbsp;Jing Wu ,&nbsp;Xiaoyi Zhu ,&nbsp;Wen Qin ,&nbsp;Mingrui Chen ,&nbsp;Yaowen Liu","doi":"10.1016/j.postharvbio.2025.113893","DOIUrl":"10.1016/j.postharvbio.2025.113893","url":null,"abstract":"<div><div>This study developed a novel microcapsule-containing thymol (THY) coating to stabilize anthocyanin accumulation in postharvest blueberries and to elucidate its underlying regulatory mechanism. The microcapsules were fabricated using β-cyclodextrin (β-CD) modified with polyethylene glycol 6000 (PEG6000) and sucrose fatty acid ester (SE-15). An orthogonal experimental design was employed to optimize the preparation process. The optimized controlled-release coating stabilized THY release while enhancing its antioxidant and antimicrobial properties. Among the coatings with different THY concentrations, the coating containing 30 mg·L⁻¹ THY (CR-30) most effectively preserved blueberry quality and promoted anthocyanin accumulation by regulating the activity of key enzymes related to anthocyanin metabolism. Integrated metabolomic and transcriptomic analyses of the CR-30 treatment identified 20 differentially accumulated metabolites (DAMs), which were enriched in anthocyanin, flavonoid, and related biosynthesis pathways, alongside 21 differentially expressed genes (DEGs) involved in anthocyanin synthesis. Furthermore, key differential transcription factors, including MYB, WRKY, and bHLH, showed significant correlations with the DAMs and DEGs, highlighting their regulatory roles in anthocyanin metabolism. This study elucidates the regulatory mechanisms of THY on anthocyanin metabolism, providing a theoretical foundation for the development of innovative postharvest quality management technologies in blueberries.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"231 ","pages":"Article 113893"},"PeriodicalIF":6.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal effect of riboflavin combined with 405 nm light-emitting diode illumination on Rhizopus stolonifer and application for tomato preservation","authors":"Won-Jun Ki ,&nbsp;Won-Seok Choi ,&nbsp;Weibiao Zhou ,&nbsp;Hyun-Gyun Yuk","doi":"10.1016/j.postharvbio.2025.113886","DOIUrl":"10.1016/j.postharvbio.2025.113886","url":null,"abstract":"<div><div>Although the antifungal effect of 405 nm light-emitting diode (LED) illumination has been documented, its limited efficacy necessitates additional strategies to enhance its performance. We aimed to improve the antifungal effect of 405 nm LED illumination by incorporating riboflavin, an exogenous photosensitizer, and evaluate its efficacy against <em>Rhizopus stolonifer</em> both <em>in vitro</em> and <em>in vivo</em> using tomatoes as a model food. The impact of the combined riboflavin and LED treatment on the physicochemical quality of tomatoes was also assessed. When three strains of <em>R. stolonifer</em> (KACC 44501, 45159, and 45160) were treated with 100 μM riboflavin in phosphate-buffered saline and exposed to LED illumination at a total dose of 2.62 kJ cm^-², only strain KACC 45160 was not reduced below the detection limit, indicating strain-dependent effects. Additionally, antifungal efficacy improved with increasing riboflavin concentrations, with the highest effect observed at 50–100 μM. Riboflavin treatment and LED illumination completely inhibited the mycelial growth of KACC 45160. LED illumination, with or without riboflavin, significantly reduced mitochondrial activity in spores, suggesting this as a potential mechanism of action. When applied to tomatoes inoculated with KACC 45160 spores, riboflavin treatment and LED illumination achieved an additional 0.8 log colony-forming units/tomato reduction compared to LED alone, without adversely affecting the physicochemical quality attributes of the tomatoes, such as color, firmness, and antioxidant capacity. These findings highlight the potential of riboflavin combined with 405 nm LED illumination as a preservation strategy to mitigate fungal spoilage in tomatoes during storage and transportation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"231 ","pages":"Article 113886"},"PeriodicalIF":6.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomic and physiological analysis highlights the candidate genes regulating enzymatic browning, antioxidant capacity, and lipid metabolism in pomegranate aril browning","authors":"Yawen Shen ,&nbsp;Sen Wang ,&nbsp;Ran Wan ,&nbsp;Jian Jiao ,&nbsp;Yujie Zhao ,&nbsp;Liu Cong ,&nbsp;Kunxi Zhang ,&nbsp;Pengbo Hao ,&nbsp;Yu Liu ,&nbsp;Wanyu Xu ,&nbsp;Miaomiao Wang ,&nbsp;Jiangli Shi ,&nbsp;Xianbo Zheng","doi":"10.1016/j.postharvbio.2025.113889","DOIUrl":"10.1016/j.postharvbio.2025.113889","url":null,"abstract":"<div><div>Pomegranate fruit is highly susceptible to chilling injury during cold storage, resulting in aril browning and the decline of nutritional value and fruit quality. However, the underlying transcriptomic mechanisms remain insufficiently understood. In this study, the physiological traits and transcriptomic data in arils were investigated after 0 h (S0), 48 h (S1), and 120 h (S2) of cold storage at 4 °C. Compared to S0, the arils in S1 and S2 exhibited significantly higher browning rate and weight loss with lower sensory quality, and contained significantly lower phenolic and flavonoid contents. In S2, arils showed oxidative stress due to hydrogen peroxide (H₂O₂) accumulation and elevated activities of polyphenol oxidase (PPO) and peroxidase (POD), potentially leading to quality deterioration. Transcriptomic analysis identified 33,822 genes, including 3397 novel genes, and highlighted coordinated changes in the key pathways, including controlling antioxidant defense, phenylpropanoid biosynthesis, and membrane lipid metabolism during aril browning. Notably, reactive oxygen species (ROS) detoxification genes (<em>PgSOD</em> and <em>PgAPX4</em>) were downregulated, while oxidative enzyme–encoding genes, including <em>PgAAO</em>, <em>PgGLO4-like</em>, <em>PgPPO</em>, and <em>PgPODs</em>, were upregulated, indicating weakened antioxidant capacity and accelerated enzymatic oxidation in aril browning. Downregulation of membrane- and cuticle-related genes (<em>PgSYT5-like</em>, <em>PgPSD1</em>, <em>PgFAR2/3</em>, and <em>PgCER1</em>) suggested that lipid biosynthesis was retarded, and cell membrane integrity was damaged. Together, aril browning during cold storage was driven by ROS imbalance, membrane damage, and oxidation. Especially, these valuable candidate genes shed light on the mechanism of fruit browning.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"231 ","pages":"Article 113889"},"PeriodicalIF":6.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Albizia [Albizia lebbeck (L.) Benth.] tree gum coating delays softening of persimmons by maintaining antioxidant system and delaying cell wall polysaccharides disassembly","authors":"Samreen Sabir,&nbsp;Shaghef Ejaz,&nbsp;Sajid Ali","doi":"10.1016/j.postharvbio.2025.113891","DOIUrl":"10.1016/j.postharvbio.2025.113891","url":null,"abstract":"<div><div>‘Fuyu’ is one of the major persimmon fruit cultivars which is widely admired due to its non-astringent nature. However, it is highly susceptible to rapid softening and quality deterioration during postharvest storage, primarily due to enhanced respiratory activity and degradation of cell wall components. This study evaluated the effect of albizia gum-based edible coating (4.5 % w/v) on the postharvest quality of persimmons stored at 20 ± 1 °C for 20 d. The results showed that albizia coating reduced weight loss, suppressed respiration rate and ethylene production, and reduced membrane permeability, as indicated by lower membrane leakage, superoxide anion, hydrogen peroxide, and malondialdehyde content. In addition, coated fruit showed substantially higher activities of antioxidant enzymes such as ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), showing improved oxidative stress mitigation. Albizia gum coating also reduced the activities of cell wall-degrading enzymes, including polygalacturonase (PG), cellulase (CS), and pectin methylesterase (PME), thereby preserving higher firmness. The coated fruit exhibited lower water-soluble pectin (WSP) and retained higher cellulose, hemicellulose, and protopectin content. The fruit coated with albizia coating also showed better biochemical and sensory attributes. These results suggest that albizia gum coating delays postharvest softening through a multifaceted mechanism involving modulation of respiratory metabolism, preserving antioxidant system activity, and suppression of cell wall disassembly. So, albizia gum coating could be applied to retard postharvest softening of persimmon during postharvest storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"231 ","pages":"Article 113891"},"PeriodicalIF":6.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser light as a novel postharvest strategy to suppress chlorophyll degradation and oxidative stress in pakchoi (Brassica rapa subsp. Chinensis)","authors":"Bo Wen ,&nbsp;Chuankai Kang ,&nbsp;Ting Yang ,&nbsp;Qi Zhan ,&nbsp;Tingyuan Chen ,&nbsp;Yulin Chen ,&nbsp;Xiaofei Wei ,&nbsp;Manuel G. Forero ,&nbsp;Xueren Yin ,&nbsp;Suriyan Supapvanich","doi":"10.1016/j.postharvbio.2025.113894","DOIUrl":"10.1016/j.postharvbio.2025.113894","url":null,"abstract":"<div><div>Short-term diode laser light irradiation is an emerging physical treatment for preserving quality and enhancing oxidative stress resilience in postharvest commodities. Rapid deterioration, including leaf yellowing, is a major factor limiting the postharvest shelf life of pakchoi. This study investigated the effects of short-term exposure to blue (450 nm) and red (660 nm) diode laser light on quality maintenance, oxidative stress mitigation, and chlorophyll metabolism in pakchoi stored at 20 °C for 5 days. The results demonstrated that 660 nm red laser irradiation significantly maintained visual quality and green coloration while reducing weight loss more effectively than 450 nm blue laser light and the untreated control. Both laser treatments reduced respiration rates and ethylene production and induced partial stomatal closure. Additionally, both treatments regulated oxidative stress responses by lowering malondialdehyde (MDA) content, lipoxygenase (LOX) activity, and reactive oxygen species (ROS) accumulation (H₂O₂ and O₂⁻), while enhancing both enzymatic and non-enzymatic antioxidant systems. The red and blue laser treatments also preserved total chlorophyll content by suppressing the expression of chlorophyll degradation genes (<em>BcPAO, BcNYC1, BcPPH1</em>) and upregulating chlorophyll biosynthesis genes (<em>BcCAO, BcCHLH</em>). Among the treatments, the 660 nm red laser exhibited the strongest effects, as confirmed by higher maximum quantum efficiency of PSII photochemistry (Fv/Fm) values and principal component analysis (PCA). Overall, these findings demonstrate that 660 nm laser irradiation synergistically modulates chlorophyll metabolism and antioxidant defense, offering an effective and practical physical strategy to extend the postharvest shelf life of leafy vegetables.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"231 ","pages":"Article 113894"},"PeriodicalIF":6.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AbZnF-GATA transcription factor synergizes with AbCYPs to regulate the browning mechanism of Agaricus bisporus","authors":"Kuo Meng ,&nbsp;Jialin Chen ,&nbsp;Wenqing Gou ,&nbsp;Lili Deng ,&nbsp;Kaifang Zeng ,&nbsp;Wenjun Wang","doi":"10.1016/j.postharvbio.2025.113890","DOIUrl":"10.1016/j.postharvbio.2025.113890","url":null,"abstract":"<div><div><em>Agaricus bisporus</em> (<em>A. bisporus</em>) is susceptible to postharvest infestation by pathogens, leading to browning and significant losses. AbZnF-GATA transcription factors and <em>AbCYPs</em> genes were found to respond positively during browning of <em>A. bisporus</em> in previous transcriptomic data, but their transcriptional regulatory roles in browning of <em>A. bisporus</em> are unknown. The aim of this study was to elucidate the biological functions of AbZnF-GATA transcription factors with nuclear membrane co-localization and <em>AbCYPs</em> genes, and their mechanisms of action during the browning process of <em>A. bisporus</em>. It was shown that <em>AbZnF-GATA</em>, <em>AbCYP52A</em>, <em>AbCYP4F12</em> and <em>AbCYP547A</em> could cause severe browning in <em>A. bisporus</em> through <em>Agrobacterium</em>-mediated transient overexpression. A possible regulatory relationship between GATA transcription factors and <em>AbCYPs</em> during browning of <em>A. bisporus</em>. In this study, it confirmed that AbZnF-GATA activates <em>AbCYP52A</em> and <em>AbCYP4F12</em> expression through a bidirectional regulatory mechanism, while inhibiting the transcriptional activity of <em>AbCYP547A</em>. It was further found that AbZnF-GATA could promote soluble quinone accumulation and on the other hand drive the browning phenotype by synergistically upregulating the catalytic activities of polyphenol oxidase (PPO), tyrosinase (TYR) and laccase (LAC). This study provides novel molecular targets for the subsequent development of postharvest browning inhibitors for <em>A. bisporus</em>.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"231 ","pages":"Article 113890"},"PeriodicalIF":6.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinctive functions of bZIP factors PeAP1 and PeAtf1 in regulating growth, virulence, stress response, and metabolism of Penicillium expansum","authors":"Yiran Wang,&nbsp;Qiya Yang,&nbsp;Kaili Wang,&nbsp;Tengwen Zhang,&nbsp;Xiaozhen Yuan,&nbsp;Hongyin Zhang","doi":"10.1016/j.postharvbio.2025.113882","DOIUrl":"10.1016/j.postharvbio.2025.113882","url":null,"abstract":"<div><div>The basic leucine zipper (bZIP) family is crucial in regulating growth, stress response and pathogenicity of phytopathogenic fungi. However, its information is limited in <em>Penicillium expansum</em>, the causal agent of apple blue mold disease. In this study, a bZIP transcription factor PeAP1 was identified in <em>P. expansum</em> through a gene replacement strategy. Results showed that Δ<em>PeAP1</em> exhibited significant growth defects, reduced virulence, and altered tolerance to osmotic, cell wall, oxidative, and cold stresses. Notably, Δ<em>PeAP1</em> showed complete growth inhibition under H₂O₂ treatment, with partial restoration of growth and pathogenicity upon antioxidant supplementation, indicating the contribution of PeAP1 to fungal growth and virulence via regulating oxidative stress response. Furthermore, we compared the role of PeAP1 and another bZIP transcription factor PeAtf1, a negative regulator of <em>PeAP1</em> and antioxidant ability, as indicated by our previous study. Results showed that both PeAP1 and PeAtf1 mediated carbon and nitrogen metabolism. PeAtf1, rather than PeAP1, positively regulates the production of patulin by controlling the patulin gene cluster. These findings provide novel insights into bZIP-mediated regulation in <em>P. expansum</em> and can help develop strategies for controlling postharvest diseases and patulin contamination caused by <em>P. expansum</em> in the future.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"231 ","pages":"Article 113882"},"PeriodicalIF":6.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A field-deployable spectroscopic approach for optimizing kiwifruit harvest timing and postharvest quality management","authors":"Te Ma ,&nbsp;Tetsuya Inagaki ,&nbsp;Satoru Tsuchikawa ,&nbsp;Hao Jiang","doi":"10.1016/j.postharvbio.2025.113888","DOIUrl":"10.1016/j.postharvbio.2025.113888","url":null,"abstract":"<div><div>Extensive research has focused on using visible-near-infrared (Vis-NIR) spectroscopy for laboratory-based quality assessment of postharvest agricultural products. Recently, field-deployable solutions are becoming increasing available, however, assessing fruit firmness remains challenging. Along with sugar content, firmness is a critical factor because of its strong influence on fruit taste and postharvest shelf life. This study designed a portable Vis–NIR spatially resolved spectroscopic (SRS) system by integrating three modules: (1) a 36-channel fiber array was arranged in six radial distances to acquire spatially resolved diffuse reflectance profiles of 108 kiwifruit samples from each of the two kiwifruit species ‘Cuixiang’ and ‘Ruiyu’ with different forchlorfenuron (CPPU) treatments, (2) a cost-effective hyperspectral imaging camera within a wavelength range of 600–1100 nm, with a resolution of 4.5 nm combined with halogen illumination was employed to reduce the measurement time of spectral data, and (3) partial least squares (PLS) regression analysis coupled with the competitive adaptive reweighted sampling technique were developed to optimize scattering-enhanced firmness prediction and scattering-suppressed soluble solids content (SSC) quantification. Field validation using the ‘Cuixiang’ species demonstrated the operational robustness of the system, with <span><math><msubsup><mrow><mi>R</mi></mrow><mrow><mi>val</mi></mrow><mrow><mn>2</mn></mrow></msubsup></math></span> = 0.65 and RMSE_val = 1.23 N for firmness and <span><math><msubsup><mrow><mi>R</mi></mrow><mrow><mi>val</mi></mrow><mrow><mn>2</mn></mrow></msubsup></math></span> = 0.65 and RMSE_val = 1 % for SSC. In contrast, for ‘Ruiyu’ species, <span><math><msubsup><mrow><mi>R</mi></mrow><mrow><mi>val</mi></mrow><mrow><mn>2</mn></mrow></msubsup></math></span> and RMSE_val were 0.14 and 2.18 N for firmness, <span><math><msubsup><mrow><mi>R</mi></mrow><mrow><mi>val</mi></mrow><mrow><mn>2</mn></mrow></msubsup></math></span> and RMSE_val values of 0.39 and 1.45 % for SSC. This could be because ‘Ruiyu’ have thicker skin and more fine hairs, which may have interfered the light reflectance measurements of the designed device. These results highlight the potential of contact SRS as an useful tool for assessing the ripeness of ‘Cuixiang’ kiwifruit, particularly in terms of firmness.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"231 ","pages":"Article 113888"},"PeriodicalIF":6.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Terpinen-4-ol enhances blueberry resistance to Alternaria via VcMYB36-mediated lignin biosynthesis","authors":"Yuxuan Li ,&nbsp;Jiaqi Wang ,&nbsp;Xunfei Zhu ,&nbsp;Runan Zhao ,&nbsp;Meihui Wen ,&nbsp;Bingxin Sun ,&nbsp;Yunhe Zhang ,&nbsp;Yufeng Xu ,&nbsp;Xuerui Yan","doi":"10.1016/j.postharvbio.2025.113892","DOIUrl":"10.1016/j.postharvbio.2025.113892","url":null,"abstract":"<div><div>Blueberry <em>Alternaria</em> fruit rot (AFR), caused by <em>Alternaria tenuissima</em>, is one of the most serious postharvest diseases affecting blueberry quality in northern China. Terpinen-4-ol (T4O), a natural monoterpene with a mild aroma, shows antifungal potential; however, its effectiveness and underlying mechanism on controlling AFR remain unclear. In this study, blueberry fruit were fumigated with 0.4 μL mL⁻¹ T4O and inoculated with <em>A. tenuissima</em>. The incidence during storage was first monitored. Transcriptome sequencing was then employed to identify differentially expressed genes (DEGs) followed by validation. Finally, the yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), and transient expression assays were conducted to further elucidate the regulatory mechanism of transcription factors on key genes. The results showed that T4O treatment reduced disease incidence by 40 % at 5 d and increased fruit firmness by 27 % compared to the control. Transcriptome analysis indicated that T4O application induced the expression of lignin biosynthesis-related genes (<em>VcCCR1</em>, <em>VcCAD</em>, and <em>VcPOD</em>) in the phenylpropanoid pathway. Among them, <em>VcCCR1</em> exhibited the highest upregulation (Log₂FC = 8.96) and was positively correlated with lignin content, suggesting its potential role in lignin accumulation in inoculated blueberries. In addition, Y1H and EMSA confirmed that the transcription factor VcMYB36 specifically bond to the CAACCA cis-element in the <em>VcCCR1</em> promoter. Transient expression assays further indicated that VcMYB36 positively regulated <em>VcCCR1</em> expression, which may contribute to lignin accumulation and improved resistance of blueberry fruit to AFR.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"231 ","pages":"Article 113892"},"PeriodicalIF":6.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}