AbZnF-GATA transcription factor synergizes with AbCYPs to regulate the browning mechanism of Agaricus bisporus

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-09-08 DOI:10.1016/j.postharvbio.2025.113890

Kuo Meng , Jialin Chen , Wenqing Gou , Lili Deng , Kaifang Zeng , Wenjun Wang

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引用次数: 0

Abstract

Agaricus bisporus (A. bisporus) is susceptible to postharvest infestation by pathogens, leading to browning and significant losses. AbZnF-GATA transcription factors and AbCYPs genes were found to respond positively during browning of A. bisporus in previous transcriptomic data, but their transcriptional regulatory roles in browning of A. bisporus are unknown. The aim of this study was to elucidate the biological functions of AbZnF-GATA transcription factors with nuclear membrane co-localization and AbCYPs genes, and their mechanisms of action during the browning process of A. bisporus. It was shown that AbZnF-GATA, AbCYP52A, AbCYP4F12 and AbCYP547A could cause severe browning in A. bisporus through Agrobacterium-mediated transient overexpression. A possible regulatory relationship between GATA transcription factors and AbCYPs during browning of A. bisporus. In this study, it confirmed that AbZnF-GATA activates AbCYP52A and AbCYP4F12 expression through a bidirectional regulatory mechanism, while inhibiting the transcriptional activity of AbCYP547A. It was further found that AbZnF-GATA could promote soluble quinone accumulation and on the other hand drive the browning phenotype by synergistically upregulating the catalytic activities of polyphenol oxidase (PPO), tyrosinase (TYR) and laccase (LAC). This study provides novel molecular targets for the subsequent development of postharvest browning inhibitors for A. bisporus.

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AbZnF-GATA转录因子与AbCYPs协同调控双孢蘑菇褐变机制

双孢蘑菇（A. bisporus）易受采后病原菌侵染，导致褐变和重大损失。在以往的转录组学研究中，AbZnF-GATA转录因子和AbCYPs基因在双孢蘑菇褐变过程中均有积极响应，但其在双孢蘑菇褐变过程中的转录调控作用尚不清楚。本研究旨在阐明核膜共定位AbZnF-GATA转录因子和AbCYPs基因在双孢酵母褐变过程中的生物学功能及其作用机制。结果表明，AbZnF-GATA、AbCYP52A、AbCYP4F12和AbCYP547A可通过农杆菌介导的瞬时过表达引起双孢酵母严重褐变。双孢酵母褐变过程中GATA转录因子与AbCYPs可能的调控关系。本研究证实AbZnF-GATA通过双向调控机制激活AbCYP52A和AbCYP4F12的表达，同时抑制AbCYP547A的转录活性。进一步发现，AbZnF-GATA一方面通过协同上调多酚氧化酶（PPO）、酪氨酸酶（TYR）和漆酶（LAC）的催化活性，促进可溶性醌积累，另一方面促进褐变表型。该研究为双孢酵母采后褐变抑制剂的后续开发提供了新的分子靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.