Postharvest Biology and Technology最新文献

{"title":"Storage temperature affects metabolism of sweet corn","authors":"Shiyu Liu ,&nbsp;Xinyuan Zhou ,&nbsp;Yunxiang Wang ,&nbsp;Qing Wang ,&nbsp;Lili Ma ,&nbsp;Cai’e Wu ,&nbsp;Ronghuan Wang ,&nbsp;Yaxing Shi ,&nbsp;Christopher B. Watkins ,&nbsp;Jinhua Zuo ,&nbsp;Yanyan Zheng","doi":"10.1016/j.postharvbio.2024.113232","DOIUrl":"10.1016/j.postharvbio.2024.113232","url":null,"abstract":"<div><div>Temperature is one of the critical factors affecting the postharvest storage quality of sweet corn, but effects of storage temperatures have not been elucidated at the molecular level. In this study, the effects of storage at 0 °C, 4 °C and 20 °C, on quality and associated effects on the transcriptome and metabolome were investigated. Compared with storage at 20 °C, cobs stored at 0 °C and 4 °C had up-regulated expression of genes associated with cell wall disassembly (<em>PG, PE, BMY, β-Gal</em>) and down-regulated expression of genes involved in lignin synthesis, the up-regulation of <em>SPP, SPS</em> and <em>SUS</em> involved in sucrose synthesis, and the down-regulation of <em>IN, FK, HXK</em> and <em>PFK</em> involved in sucrose catabolism. Most of the genes involved in ethylene biosynthesis and signaling were inhibited at 0 °C, except for <em>ERFs</em>, while abscisic acid synthesis and signal transduction were most active at this temperature. Near-freezing temperature also maintains the integrity of the cell membrane by inhibiting the expression of <em>PLD, PLC</em> and <em>LOX</em>, which encode membrane lipid-degrading enzymes. Metabolomics and combined analysis showed that the contents of lysophospholipid, glutathione and L-ascorbic acid in sweet corn increased at low temperatures. These results provide insight into the effects of low temperatures on sweet corn quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113232"},"PeriodicalIF":6.4,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ursolic acid, a natural endogenous compound, inhibits browning in fresh-cut apples","authors":"Song Zhang ,&nbsp;Shuwen Wang ,&nbsp;Yu Li,&nbsp;Jinjie Wang,&nbsp;Jingying Shi,&nbsp;Yong Peng,&nbsp;Pei Liu","doi":"10.1016/j.postharvbio.2024.113228","DOIUrl":"10.1016/j.postharvbio.2024.113228","url":null,"abstract":"<div><div>Enzymatic browning occurs rapidly in fresh-cut apples and can lead to economic losses. Although various methods have been developed to prevent this browning, physical methods are costly, and chemical methods are controversial, suggesting the potential usefulness of an endogenous natural browning inhibitor. In this study, the effects of ursolic acid (UA), a natural endogenous compound, on apple browning were investigated. It was found that UA inhibited the activity of polyphenol oxidase through Cu²⁺ chelation. Molecular docking indicated that the inhibition was reversible and mixed-type. Moreover, UA treatment promoted the scavenging of reactive oxygen species in fresh-cut apple and reduced the proliferation of microorganisms on the apple surface. Similar effects were observed on the browning of chestnut, eggplant, potatoes, and pears. In conclusion, the findings demonstrate the efficacy of UA on inhibiting browning in fresh-cut apple, together with an analysis of the mechanism involved.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113228"},"PeriodicalIF":6.4,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of terpene synthase genes accounting for volatile terpene emissions in the flower of Paeonia lactiflora","authors":"Qian Zhao ,&nbsp;Yuqing Li ,&nbsp;Lina Gu ,&nbsp;Shuting Li ,&nbsp;Di He ,&nbsp;Shanrong Dong ,&nbsp;Qingyu Zhang ,&nbsp;Jianrang Luo ,&nbsp;Yanlong Zhang","doi":"10.1016/j.postharvbio.2024.113231","DOIUrl":"10.1016/j.postharvbio.2024.113231","url":null,"abstract":"<div><div>Emergence of floral scents marked a significant milestone in biological development of plant, letting out olfactory signals that enable them to induce pollinators. In this research, molecular and metabolomics methodologies were used to explore terpene synthase (TPS) genes, which are responsible for synthesis of floral fragrance in <em>Paeonia lactiflora</em> ‘Wu Hua Long Yu’. Analyses on transcriptome data identified 11 <em>PlTPS</em> genes, among them 7 genes were isolated, and the functions of 5 genes were confirmed. <em>PlTPS1</em> was identified as a single-product enzyme; <em>PlTPS4</em>, <em>8</em> and <em>9</em> were identified as bifunctional enzymes, capable of recognizing farnesyl pyrophosphate and geranyl pyrophosphate; <em>PlTPS6</em> generated multiple products by recognizing farnesyl pyrophosphate. Enzymatic products of these PlTPS proteins closely matched the volatile terpenes emitted by flowers, revealing the relation between <em>PlTPS</em> genes and the release of such volatile compounds. Functions of five <em>PlTPS</em> genes in herbaceous peony were further elucidated through virus-induced gene silencing experiments, as well as overexpression in tobacco. <em>PlTPS1</em> catalyzes geraniol production, while <em>PlTPS4</em> catalyzes linalool. <em>PlTPS6</em> is responsible for producing two sesquiterpenes, caryophyllene and α-humulene. <em>PlTPS8</em>, with the most varied products, mainly catalyzes the production of α-pinene, linalool, and germacrene D. Based on these findings, graphical models summarizing the biosynthesis of floral volatile terpenes in herbaceous peony have been proposed. The characterization of <em>PlTPS</em> genes has elucidated their specific roles in the synthesis of herbaceous peony fragrance, opening up possibilities for introducing or enhancing floral scents in herbaceous peony and other plants.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113231"},"PeriodicalIF":6.4,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a unified framework of low-rank approximation and deep neural networks for predicting the spatial variability of SSC in `Spania' watermelons using vis/NIR hyperspectral imaging","authors":"Jobin Francis ,&nbsp;Sony George ,&nbsp;Binu M. Devassy ,&nbsp;Sudhish N. George","doi":"10.1016/j.postharvbio.2024.113222","DOIUrl":"10.1016/j.postharvbio.2024.113222","url":null,"abstract":"<div><div>Soluble Solids Content (SSC) is an important quality attribute that represents the internal quality of fruits. Visible and near-infrared (Vis/NIR) combined with chemometric algorithms are now popular methods for non-invasive measurement and visualization of SSC. However, in fruits with a thick rind and a large flesh volume, such as watermelon, SSC is not evenly distributed across the fruit. The variability in SSC across the fruit flesh may have an adverse effect on the accuracy of quality analysis algorithms. Thus, this paper presents an accurate and efficient approach for predicting the spatial variation of SSC in watermelons using a combined framework of low-rank approximation and deep neural networks. Watermelon ‘Spania’ was selected for this study, and hyperspectral images of each watermelon were taken from various views, including the top, bottom, and two lateral views. The low-rank property is employed as a constraint in this approach to eliminate the unwanted variations in the spectral data. The low-rank component of the spectral data, free of unwanted variations, is then fed into a fully connected neural network (FNN) for the prediction of watermelon SSC values. The proposed approach obtained optimal performance in calibration and prediction with <span><math><mrow><msubsup><mrow><mspace></mspace><mtext>R</mtext><mspace></mspace></mrow><mrow><mi>C</mi></mrow><mrow><mn>2</mn></mrow></msubsup><mo>=</mo><mn>0.982</mn></mrow></math></span>, RMSEP = 0.132, and <span><math><mrow><msubsup><mrow><mspace></mspace><mtext>R</mtext><mspace></mspace></mrow><mrow><mi>P</mi></mrow><mrow><mn>2</mn></mrow></msubsup><mo>=</mo><mn>0.945</mn></mrow></math></span>, RMSEP = 0.195 respectively. Further, the prediction outcomes of the proposed method were compared with state-of-the-art such as Partial Least Square Regression (PLSR), Support Vector Regression (SVR), Multiple Linear Regression (MLR), Decision Tree (DT), and Random Forest (RF). The overall results showed that combining HSI data with a low-rank and deep neural network framework is an efficient method for accurately predicting SSC in watermelons as well as correctly predicting spatial variability of SSC in individual fruits.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113222"},"PeriodicalIF":6.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and metabolic insights into the mechanism of exogenous methyl jasmonate in enhancing the postharvest resistance of kiwifruit to Botrytis cinerea","authors":"Jiaqi Yang ,&nbsp;Yijia Ma ,&nbsp;Tianjing Zeng,&nbsp;Zhexin Li,&nbsp;Yuan Sui,&nbsp;Wenlin Zhang,&nbsp;Hongpan Zhong,&nbsp;Xu Wang","doi":"10.1016/j.postharvbio.2024.113226","DOIUrl":"10.1016/j.postharvbio.2024.113226","url":null,"abstract":"<div><div>Gray mold, caused by <em>Botrytis cinerea</em> infection, significantly impacts postharvest kiwifruit, leading to spoilage and food safety issues. Meanwhile, methyl jasmonate (MeJA) induces defense responses in plants. This study aimed to identify the optimal MeJA concentration to induce disease resistance in kiwifruits. Notably, various plant defense enzymes were detected in MeJA-treated kiwifruit. Moreover, 0.01 mol/L MeJA was found to enhance <em>B. cinerea</em> resistance in kiwifruit by increasing antioxidant enzyme activity. Widely targeted metabolomics analysis revealed 62–64 differentially expressed metabolites (DEMs) between the different treatment groups. Additionally, RNA-seq analysis revealed 930–2900 differentially expressed genes (DEGs), between these groups. Subsequently, combined DEG and DEM analysis highlighted phenylpropanoid biosynthesis and plant hormone signaling as key transduction pathway associated with MeJA-induced resistance. Overall, these findings identified the mechanism of MeJA-induced resistance in kiwifruit, providing a theoretical basis for the safe and effective control of postharvest diseases in kiwifruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113226"},"PeriodicalIF":6.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine localization and functional differentiation of exocarp cell clusters during fruit senescence revealed by single-cell and spatial transcriptomics","authors":"Jingyu Jia ,&nbsp;Enyan Chen ,&nbsp;Yajing Tian ,&nbsp;Jiahao Shen ,&nbsp;Xinyue Pang ,&nbsp;Xin Li","doi":"10.1016/j.postharvbio.2024.113223","DOIUrl":"10.1016/j.postharvbio.2024.113223","url":null,"abstract":"<div><div>The differentiation trajectories of 13 cell clusters in <em>H. undatus</em> during senescence have been revealed by single-cell transcriptome (scRNA-seq) analysis, and the five cell clusters in the exocarp have been found to induce resistance mainly. However, the precise localization and functional differentiation of these five cell clusters remain unclear. In this study, phenotypic changes in the exocarp were recorded, and the ROS production and flavonoid biosynthesis in the exocarp were elucidated. The trajectories of the cell clusters involved in exocarp function were further revealed in scRNA-seq profiles. Through integrated analysis of scRNA-seq and spatial RNA-seq data at resolutions of 0.5, 0.6, and 1.5, using algorithms SingleR, SciBet, CARD, and RCTD, the fine localization of these clusters from the outer to inner layers was achieved. RNA-FISH was used to validate the specific expression of marker genes <em>HuSP2</em> and <em>HuCEL2</em> in different locations of the exocarp. Finally, the differentiation trajectories of the five cell clusters were analyzed by selecting highly correlated cells with specific functions for each cluster. The core roles of <em>HuPRP</em> and <em>HuCRRSP55</em> in the gene regulatory networks of exocarp clusters were revealed. A hypothesis that the five clusters in the exocarp participate in the induction of resistance was proposed.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113223"},"PeriodicalIF":6.4,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S092552142400468X/pdfft?md5=6510d0cfd8ff1e5594ec82e61fdcac1e&pid=1-s2.0-S092552142400468X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CpAGL18 is involved in the cross-talk of ethylene and auxin signaling in the regulation of fruit ripening in papaya","authors":"Jiahui Cai ,&nbsp;Ziling Wu ,&nbsp;Wang Zheng ,&nbsp;Qiunan Zhu ,&nbsp;Zunyang Song ,&nbsp;Weixin Chen ,&nbsp;Xueping Li ,&nbsp;Xiaoyang Zhu","doi":"10.1016/j.postharvbio.2024.113221","DOIUrl":"10.1016/j.postharvbio.2024.113221","url":null,"abstract":"","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113221"},"PeriodicalIF":6.4,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142651001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of pH and temperature in a heated flooder application on imazalil residue loading, citrus green mould infection and sporulation inhibition","authors":"Catherine Savage ,&nbsp;Arno Erasmus ,&nbsp;Wilma du Plooy ,&nbsp;Cheryl Lennox ,&nbsp;Paul H. Fourie","doi":"10.1016/j.postharvbio.2024.113227","DOIUrl":"10.1016/j.postharvbio.2024.113227","url":null,"abstract":"<div><div>Green mould (caused by <em>Penicillium digitatum</em>) is the largest contributor to loss due to postharvest decay of citrus fruit. Imazalil (IMZ) in the IMZ sulphate formulation is the most important fungicide in green mould management in citrus packhouses. Studies have highlighted the importance of pH and temperature of IMZ sulphate solutions, as well as the exposure time of fruit to the solution, but were focussed on dip application as this was the most common IMZ application method. The heated flooder (in-line drench or cascade) has widely replaced the use of a dip or bath for IMZ application in citrus packhouses globally; however, studies on its optimal use and efficacy were needed. Variables that were studied included the effects of pH (3, 4, 5 and 6), the temperature of the solution (45, 55 and 65 °C at pH 6), and concentration (250 or 500 mg L⁻¹) in a time of 8 s. Imazalil residue loading models were developed for the effects of pH and temperature. Imazalil residue levels loaded on Satsuma mandarin, lemon, navel and Valencia orange fruit increased as pH, temperature range and concentration were increased, and were generally in the range between 0.4 and 3.0 mg kg⁻¹. The South African Maximum Residue Limit (MRL) of 5.0 mg kg⁻¹ was often exceeded at a pH of 6 at temperatures of 55 and 65 °C. At residue levels below the MRL, IMZ application using the heated flooder offered excellent curative action on 24-hour-old infections, excellent protective control, as well as sporulation inhibition. Sporulation inhibition of <em>P. digitatum</em> from green mould lesions was modelled on residue levels, and 90 % inhibition was obtained at residue levels of 1.84–2.47 mg kg⁻¹.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113227"},"PeriodicalIF":6.4,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0925521424004721/pdfft?md5=1b9105a45df6abfbdf5b9d51abe4ad68&pid=1-s2.0-S0925521424004721-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous deferoxamine mesylate suppresses iron-dependent ferroptosis in postharvest fruit rot of blueberry caused by Alternaria alternata and Fusarium fujikuroi","authors":"Shuang Gu ,&nbsp;Lin Xie ,&nbsp;Xuerong Sheng ,&nbsp;Guiqing Xing ,&nbsp;Xiangyang Wang","doi":"10.1016/j.postharvbio.2024.113216","DOIUrl":"10.1016/j.postharvbio.2024.113216","url":null,"abstract":"<div><div>Plant development relies heavily on regulated cell death, which is crucial for specific plant responses to biological stresses. Ferroptosis, an iron-dependent, oxidative, and non-apoptotic cell death form, has been recently discovered in animal cells. In this study, we investigated whether a ferroptosis-like process could be relevant to cell death in postharvest fruit rot of blueberry caused by <em>Alternaria alternata</em> and <em>Fusarium fujikuroi</em>. The results showed that deferoxamine mesylate and glutathione (GSH) treated fruit showed enhanced disease resistance with lower lesion areas, whereas an opposite result was observed in hydrogen peroxide (H₂O₂) treatment, indicating that intracellular ferroptosis might exist in the fungal infected blueberry fruit. Compared to control, deferoxamine mesylate or GSH treatment improved chlorophyll fluorescence parameters, which contributed to the stability of photosynthesis. In terms of ferroptosis pathway in the adjacent area of infected fruit, deferoxamine mesylate suppressed an iron-dependent cell death pathway that was characterized by depletion of H₂O₂ and MDA and accumulation of GSH, as well as the reduction of ferrous ions and total iron ions. Meanwhile, deferoxamine mesylate could effectively delay the decrease rate of unsaturation value and key phospholipids of fungi infected fruit during storage, which possibly maintained the integrity of cell membrane. Due to the serious rot of the lesion area, the peroxidation of membrane lipids was severe, accompanied by metabolic disorders. These combined results demonstrated that deferoxamine mesylate treatment could reduce Fenton reaction by chelating Fe²⁺ and avoid excessive accumulation of reactive oxygen species (ROS), suppress iron-dependent ferroptosis, thereby maintaining fruit quality and reducing postharvest diseases.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113216"},"PeriodicalIF":6.4,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0925521424004617/pdfft?md5=b6ef843587ce115d6507465b24a1f71f&pid=1-s2.0-S0925521424004617-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methyl jasmonate activated regulatory module Ma14-3-3e-MbHLH130-MbACO13/MbACS7 promoting ethylene biosynthesis and fruit ripening in banana","authors":"Meiying Li ,&nbsp;Yan Yan ,&nbsp;Liwang Zeng ,&nbsp;Zhengnan Xie ,&nbsp;Zehong Ding ,&nbsp;Jinghao Yang ,&nbsp;Yu Wang ,&nbsp;Jianxiang Ma ,&nbsp;Kaisen Huo ,&nbsp;Xiaoliang Yang ,&nbsp;Qiyu Xia ,&nbsp;Xiao xue Ye ,&nbsp;Chaochao Li ,&nbsp;Zhiqiang Jin ,&nbsp;Licheng Ren ,&nbsp;Wei Hu","doi":"10.1016/j.postharvbio.2024.113215","DOIUrl":"10.1016/j.postharvbio.2024.113215","url":null,"abstract":"<div><p>Methyl jasmonate (MeJA), an important phytohormone, plays a vital role in many biological processes. However, its effect and mechanism in regulating ethylene synthesis and fruit ripening in banana remain unknown. In this research, we found that exogenous MeJA accelerated postharvest ripening of banana, which coincided with increased ethylene production and upregulation of <em>MbACS7</em> and <em>MbACO13</em> expression. MabHLH130 directly interacted with and stimulated the transcription of <em>MbACS7</em> and <em>MbACO13</em>. Additionally, MabHLH130 interacted with Ma14-3-3e through the RHSSSP motif. Overexpression of either <em>MabHLH130</em> or <em>Ma14-3–3e</em> in tomato promoted ethylene production and fruit ripening, and this effect was enhanced by exogenous MeJA treatment. Furthermore, the Ma14-3–3e-MabHLH130 interaction module activated <em>MbACS7</em> and <em>MbACO13</em> transcription, which was enhanced by exogenous MeJA treatment. Taken together, these results demonstrated that the MeJA-responsive Ma14-3–3e-MabHLH130-<em>MbACS7</em>/<em>MbACO13</em> module promoted ethylene biosynthesis and fruit ripening in bananas, providing valuable genetic targets for breeding programs aimed at extending fruit shelf-life.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113215"},"PeriodicalIF":6.4,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0925521424004605/pdfft?md5=491c6b75050a77a3bb6764b1e1140a68&pid=1-s2.0-S0925521424004605-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}