Postharvest Biology and Technology最新文献

{"title":"Dynamic analysis of fungal community and metabolome associated with postharvest diseases in banana peel under natural condition","authors":"Ping Yi ,&nbsp;Ming Zhao ,&nbsp;Fang Huang ,&nbsp;Li Li ,&nbsp;Jian Sun ,&nbsp;Lihong Xie ,&nbsp;Dongning Ling ,&nbsp;Jiemin Li ,&nbsp;Min Huang ,&nbsp;Ting Gan ,&nbsp;Yayuan Tang ,&nbsp;Changbao Li ,&nbsp;Xuemei He ,&nbsp;Jinfeng Sheng","doi":"10.1016/j.postharvbio.2024.113384","DOIUrl":"10.1016/j.postharvbio.2024.113384","url":null,"abstract":"<div><div>Bananas (<em>Musa acuminate</em>) are one of the most affordable and popular fruits in the world, and the fourth most important harvest crop after rice, wheat and maize. Due to the effects of physiological changes, flesh softening, and microbial invasion, and other factors, postharvest losses of bananas during transportation and sales exceed 25 %. Fungal infection of bananas leads to huge fruit loss and economic damage during postharvest period. To assess banana decay and related fungal communities under typical storage conditions, representative samples were collected and detected at J1–5 (0–20 D) time points after harvest. Metabolomic changes, temporal profiles of metabolites, and interaction networks between key fungi and their metabolites were explored. A sharp increase in yellow color was observed at J4 when peel browning appeared, and the commercial value was significantly reduced at J5. Fruit firmness gradually decreased over the experimental stages, whereas total soluble solids (TSS), titratable acidity (TA), and malondialdehyde (MDA) content gradually increased. The results revealed that Ascomycota was the dominant phylum, followed by Basidiomycota. <em>Neocucurbitaria</em> exhibited a higher relative abundance at J2 and J3, while <em>Nigrospora</em> and <em>Cladosporium</em> were the dominant genera at J4. In addition, the abundances of <em>Colletotrichum</em>, <em>Golubevia</em>, <em>Moesziomyces</em>, <em>Acremonium</em>, <em>Neopyrenochaeta</em>, and <em>Penicillium</em> at J5 were significantly higher than those at the other time points. The largest change was observed for comparison J3-vs-J4 with a total of 59 differential metabolites, followed by J4-vs-J5 with a total of 51 differential metabolites. The differential metabolites of J3-vs-J4 were mainly involved in carbohydrate metabolism-related pathways and membrane transport-related pathways, whereas J4-vs-J5 were largely enriched in secondary metabolite synthesis-related pathways. Moreover, profile 19 was significantly enriched in multiple pathways associated with carbohydrate metabolism. Finally, an interaction network revealed that <em>Acremonium</em>, <em>Colletotrichum</em>, and <em>Nigrospora</em> were significantly positively correlated with most of the differential metabolites. It should be highlighted that <em>Colletotrichum</em> promoted the occurrence of anthracnose during postharvest by upregulating myo-inositol. This study hold profound implications for enhancing our understanding of fungal community and metabolome associated diseases in the postharvest stage provided a valuable strategy of banana preservation based on pathogenic fungal inhibition and metabolite regulation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113384"},"PeriodicalIF":6.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing regulatory mechanism of AdMYB52 transcription factor in callusing-suberin biosynthesis in kiwifruit","authors":"Xueyuan Han ,&nbsp;Chenhao Cao ,&nbsp;Jie Han ,&nbsp;Chaogeng Xiao ,&nbsp;Wenjing Lu ,&nbsp;Lijia Dong ,&nbsp;Jiayao Fu ,&nbsp;Huangjin Zhu","doi":"10.1016/j.postharvbio.2024.113375","DOIUrl":"10.1016/j.postharvbio.2024.113375","url":null,"abstract":"<div><div>Callusing-suberin in kiwifruit is induced by mechanical friction during harvesting and transportation. This research identified AdMYB52 transcription factor (TF) as a regulator of callusing-suberin biosynthesis. Transient overexpression of <em>AdMYB52</em> in kiwifruit callus increased suberin accumulation, while transient gene silencing resulted in decreased suberin levels. Using DNA affinity purification sequencing (DAP-seq), 3273 peak-related genes and 123 significantly enriched motifs were identified as potential binding sites of AdMYB52. Further combining analysis of DAP-seq and RNA sequencing (RNA-seq) identified 343 overlapping genes as potential targets of AdMYB52. Gene ontology (GO) analysis revealed that these target genes are enriched in processes related to fatty acid metabolism, oxidoreductase activity, cell cortex formation, and phenylalanine catabolic process, all of which are associated with suberin synthesis. Electrophoretic mobility shift assay (EMSA) verified AdMYB52 binding to six motifs in the promoters of target genes (<em>KCS, ABCB1, LPS, CYP724B1, FAR, LACS</em>) involved in suberin biosynthesis. The analysis of qRT-PCR further confirmed the regulation of these target genes by AdMYB52. This study elucidates a possible regulatory mechanism for callusing-suberin biosynthesis involving AdMYB52 TF.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113375"},"PeriodicalIF":6.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143092661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disorder of cell wall metabolism during the transition of citrus juice sacs from healthy to pre-granulation and granulation stages: Evidence from Shiranui mandarin","authors":"Meizhu Huang ,&nbsp;Chunlian Huang ,&nbsp;Jiao Hou ,&nbsp;Kaifang Zeng ,&nbsp;Shixiang Yao","doi":"10.1016/j.postharvbio.2024.113383","DOIUrl":"10.1016/j.postharvbio.2024.113383","url":null,"abstract":"<div><div>Citrus granulation is the major physiological disorder that adversely affects fruit quality during postharvest senescence. The \"Disorder of cell wall metabolism\" hypothesis has proposed that cell wall metabolism disorder may drive nutrient depletion and juice sacs granulation, primarily based on comparisons between non-granulated and granulated juice sacs. However, the metabolic changes during the transition from healthy to pre-granulation and granulation stages remain unexplored. Hence, this study comprehensively analyzed metabolites, enzyme activities, and gene expressions of juice sacs from Shiranui mandarin at these stages. Cell wall components, including cellulose and chelate-soluble pectin, progressively increased from healthy to pre-granulation and granulation stages, while lignin content remained stable. Concurrently, sugars and citrate decreased significantly in the granulation stage, with glucose and citrate beginning in the pre-granulation stage. Transcriptional activation and increased precursors for lignin synthesis were observed in the pre-granulation stage and intensified in the granulation stage. Key genes for cellulose synthesis were also activated, pectin demethylesterification was initiated, and pectin degradation was repressed starting from the pre-granulation stage, and intensified in the granulation stage. Transcriptional repression of sucrose synthesis and activation of sucrose degradation began in the pre-granulation stage and intensified in the granulation stage. Citrate degradation was driven by suppressed synthesis, and enhanced degradation via the acetyl-CoA and glutamine pathways, initiating in the pre-granulation stage and intensifying in the granulation stage. No energy deficiency was observed in juice sacs in either stage. Overall, this study is the first to provide direct evidence that cell wall metabolism disorder initiates before granulation and becomes more pronounced during granulation, supporting its role in driving citrus granulation and expanding the “Disorder of cell wall metabolism” hypothesis. These findings offer novel insights into the mechanism underlying citrus fruit senescence.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113383"},"PeriodicalIF":6.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143092658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of polyethylene glycol on reducing mechanical damage in white mushrooms during simulated logistics vibrations and storage","authors":"Zhengli Wang ,&nbsp;Nana Ji ,&nbsp;Mei Yang ,&nbsp;Ying Chen ,&nbsp;Yanyin Guo ,&nbsp;Rinkiko Suguro","doi":"10.1016/j.postharvbio.2024.113377","DOIUrl":"10.1016/j.postharvbio.2024.113377","url":null,"abstract":"<div><div>White mushrooms are the most widely cultivated edible mushrooms globally, but quality degradation during logistics limits their international and domestic trade potential. This study investigated the efficacy of polyethylene glycol (PEG) in mitigating mechanical damage caused by logistic vibrations during the storage of white mushrooms. The results showed that 0.3 % PEG effectively maintained the quality attributes, including elasticity, total phenolics, flavonoids, lignin, and chitin, thereby enhancing the resistance of white mushrooms to vibrational stress. PEG of 0.3 % concentration increased the antioxidant system, exhibited moderate contents of superoxide anion and hydrogen peroxide and higher levels of superoxide dismutase and catalase activities. Furthermore, the enhanced antioxidant system ensured the phenylpropanoid metabolism pathway by increasing the activities of phenylalanine ammonia-lyase (PAL), 4-coumarate: CoA ligase (4CL) and cinnamate 4-hydroxylase (C4H). This pathway is associated with plant resistance, promotes wound healing and increase cellular resistance. This process accelerated the lignification of damaged cells and enhanced the cell wall hardness and compressive strength, thereby mitigating mechanical damage caused by vibration. Meanwhile, the high humidity environment during storage favored the recovery of osmotic stress in white mushrooms, which enhanced their resistance to vibrational stress. Consequently, the application of 0.3 % PEG effectively mitigated mechanical damage in white mushrooms during logistics and transport, providing a novel approach to their storage and handling.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113377"},"PeriodicalIF":6.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143092662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of nitric oxide and extremely low oxygen storage on volatile organic compounds and overall quality of ‘Maxi Gala’ apples","authors":"Francis Júnior Soldateli ,&nbsp;Carine Borges Batista ,&nbsp;Bruna Regina Carvalho Pinto ,&nbsp;Lucas Mallmann Wendt ,&nbsp;Barbara Cecconi Deon ,&nbsp;Vagner Ludwig ,&nbsp;Fabio Rodrigo Thewes ,&nbsp;Cristiano André Steffens ,&nbsp;Auri Brackmann ,&nbsp;Vanderlei Both","doi":"10.1016/j.postharvbio.2024.113374","DOIUrl":"10.1016/j.postharvbio.2024.113374","url":null,"abstract":"<div><div>This study aimed to evaluate the effect of nitric oxide (NO) application on overall quality and volatile compounds of ‘Maxi Gala’ apples stored under controlled atmosphere (CA) and with extremely low oxygen (ELO). The fruit were treated or not with NO at the beginning and end (10 + 10 µL L⁻¹), or only at the end of storage (10 µL L⁻¹) and, were stored under the following conditions: [1] CA (1.2 kPa O₂; 1.6 kPa CO₂) + 1-methylcyclopropene (CA + 1-MCP); [2] ELO (0.4 kPa O₂; 1.6 kPa CO₂); [3] ELO + 0.4 hysteresis; [4] ELO + 0.6 hysteresis and; [5] dynamic controlled atmosphere by respiratory quotient (DCA-RQ 1.3; 1.6 kPa CO₂). After 7.5 months storage, plus 7 d shelf life, the fruit quality was evaluated. Fruit stored under ELO exhibited higher ACC oxidase activity, ethylene production, and respiration rate. ELO + 0.6 hysteresis maintained higher flesh firmness without NO application and suppressed total esters content similarly to CA + 1-MCP, but with less impact on 2-methylbutyl acetate. NO application at the beginning and end of storage resulted in higher concentrations of aldehydes, alcohols, and esters, but it reduced the concentration of phenolic compounds and flesh firmness, and increased the incidence of mealiness in CA + 1-MCP and DCA-RQ. NO applied only at the end of storage did not improve fruit quality. Thus, the reapplication of NO should be better evaluated under extremely low oxygen storage, although it can be a promising strategy to enhance the aromatic quality of fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113374"},"PeriodicalIF":6.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143092663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sucrose induces BoVDAC2 expression to preserve mitochondrial function and mitigate programmed cell death in broccoli","authors":"Min Luo ,&nbsp;Jiahui Chen ,&nbsp;Shifeng Cao ,&nbsp;Xintong Guo ,&nbsp;Yuan Qian ,&nbsp;Wenhui Jiao ,&nbsp;Hangjun Chen ,&nbsp;Yi Chen ,&nbsp;Yingying Wei ,&nbsp;Xingfeng Shao ,&nbsp;Feng Xu","doi":"10.1016/j.postharvbio.2024.113382","DOIUrl":"10.1016/j.postharvbio.2024.113382","url":null,"abstract":"<div><div>The alleviation of the impact of sucrose treatment on programmed cell death (PCD) in postharvest broccoli has been reported in previous studies; however, the fundamental mechanisms are still not fully understood. This study found that exogenous sucrose treatment maintained the mitochondrial structure in broccoli buds, enhanced mitochondrial membrane potential (ΔΨm), elevated activity of antioxidant enzymes and decreased levels of reactive oxygen species (ROS). These changes ultimately inhibited the release of Cytochrome c/a (Cytc/a) and delayed PCD in broccoli buds. Furthermore, the expression of <em>BoVDAC2</em> progressively decreased with prolonged storage. To further explore the relationship between <em>BoVDAC2</em> and PCD, <em>BoVDAC2</em> was overexpressed in BY-2 cells in this study. Compared to wild-type cells, <em>BoVDAC2</em>-overexpressing cells showed reduced mitochondrial ultrastructure damage, increased cell vitality, enhanced antioxidant enzyme activity, and decreased ROS production. Additionally, <em>BoVDAC2</em> overexpression elevated energy metabolism, maintained mitochondrial membrane integrity, inhibited Cytc/a release, suppressed caspase-like activity, and improved the expression of genes related to PCD in BY-2 cells. Overall, the findings suggest that sucrose induces <em>BoVDAC2</em> expression, which helps to maintain mitochondrial structure and function in postharvest broccoli buds, thereby mitigating PCD.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113382"},"PeriodicalIF":6.4,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143092660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioimpedance-based prediction of dry matter content and potato varieties through supervised machine learning methods","authors":"Ciro Allará ,&nbsp;Roberto Moscetti ,&nbsp;Giacomo Bedini ,&nbsp;Manuela Ciocca ,&nbsp;Alessandro Benelli ,&nbsp;Paolo Lugli ,&nbsp;Luisa Petti ,&nbsp;Pietro Ibba","doi":"10.1016/j.postharvbio.2024.113358","DOIUrl":"10.1016/j.postharvbio.2024.113358","url":null,"abstract":"<div><div>This study explored bioimpedance measurements and their derived parameters as potential indicators of dry matter content and potato variety. We investigated how bioimpedance correlates with the dry matter composition of potatoes,through destructive tests on tissue slices. Using the machine learning approach, we identified patterns and associations that can aid in predicting dry matter content, using regression models, and discriminating among potato varieties, focusing on classification models. In particular, four feature selection methods (correlation matrix, minimum-redundancy–maximum-relevance, neighborhood component analysis, and t-test) were evaluated against a baseline with all features. It was found that the best regression model for dry matter was a neural network regression model with t-test features, which achieved <span><math><msup><mrow><mi>R</mi></mrow><mrow><mn>2</mn></mrow></msup></math></span> of 0.62 and RMSE of 2.3% in testing, while the best classification model for potato variety was a neural network classification model with correlation matrix features, achieving an <span><math><msub><mrow><mi>F</mi></mrow><mrow><mn>1</mn></mrow></msub></math></span> score of 0.92.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113358"},"PeriodicalIF":6.4,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanism of the transcription factor ZjWRKY55 in regulating resistance to black spot rot in jujube fruit","authors":"Xuan Zhang ,&nbsp;Wenjun Wang ,&nbsp;Yao Liu ,&nbsp;Jialin Chen ,&nbsp;Kaifang Zeng","doi":"10.1016/j.postharvbio.2024.113353","DOIUrl":"10.1016/j.postharvbio.2024.113353","url":null,"abstract":"<div><div><em>Ziziphus jujuba</em>, with its thin and crispy skin, sweet taste, and high economic and nutritional value, is a popular fresh fruit. Black spot rot, instigated by <em>Alternaria alternata</em> (<em>A. alternata</em>), stands as a primary post-harvest affliction impacting jujube. Compared to traditional fungicides, amino acid treatment as a biological control method is safer and environmentally friendly, without any harm to humans or the environment. Previous studies have shown that treating jujube fruit with methionine (Met) can enhance the enzymatic activity of lignin synthesis-related enzymes and increase the expression of transcription factors (TFs) such as ZjWRKY55, thereby improving the resistance of jujube fruit. This study mainly focuses on investigating the mechanism of how the transcription factor ZjWRKY55 regulates the resistance of jujube fruit. Transient overexpression of <em>ZjWRKY55</em> in jujube significantly reduces the lesion diameter and incidence rate of black spot rot and significantly increases the lignin content in jujube fruit. RNA sequencing (RNA-seq) analysis of the transiently transformed samples reveals significant enrichment of differentially expressed genes involved in pathways such as sugar metabolism, amino acid metabolism, phenylpropane biosynthesis, and alpha-linolenic acid metabolism. Real-time fluorescence quantitative PCR (RT-qPCR) indicates upregulated expression of genes such as <em>ZjCCoAOMT, ZjGAD, ZjCAD, ZjPER,</em> and <em>ZjBGLU</em>. Dual-luciferase reporter (DLR) assays and electrophoretic mobility shift (EMSA) assays demonstrate that <em>ZjWRKY55</em> can directly bind to the upstream promoters of <em>ZjCCoAOMT</em> and <em>ZjGAD</em> to activate their transcriptional expression and bind to the promoter of <em>ZjKTI5</em> to downregulate its expression.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113353"},"PeriodicalIF":6.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143092605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postharvest fragrance dynamics of different scented cut lilies: Insights from HS–SPME–GC–MS and electronic nose","authors":"Yiwei Zhou ,&nbsp;Yiwei Chen ,&nbsp;Jinfeng Dong ,&nbsp;Qin Wang ,&nbsp;Fulong Yan ,&nbsp;Jianjun Tan ,&nbsp;Yechun Xu ,&nbsp;Genfa Zhu ,&nbsp;Yanping Fan ,&nbsp;Yuanjun Ye","doi":"10.1016/j.postharvbio.2024.113378","DOIUrl":"10.1016/j.postharvbio.2024.113378","url":null,"abstract":"<div><div>Lilies (<em>Lilium</em> spp.) are important scented cut flowers, with fragrance being one of their most crucial ornamental traits. However, there are few reports on the rapid and efficient detection of postharvest fragrance quality in cut lilies. This study evaluated the floral scents of ten cut lily cultivars at four stages of aging using HS–SPME–GC–MS and an electronic nose. A total of 51 volatile organic compounds (VOCs) were detected by HS–SPME–GC–MS. Monoterpenoids were the predominant compounds in the fragrances of 'White Present', 'Bai Xue Gong Zhu', 'Manissa', 'Sorbonne', 'Touchstone', 'Robina', and 'Yelloween', while benzenoids/phenylpropanoids were predominant in 'Cali', 'Roselily Isabella', and 'Wanxia'. The electronic nose sensors W1W, W2W, and W5S were more sensitive to the floral scents of these cut lily cultivars. Correlation analysis indicated good consistency between the GC–MS and E–nose detection results (<em>r</em> = 0.79, <em>p</em> &lt; 0.01). PLS–DA and Random Forest analyses highlighted (<em>E</em>)-<em>β</em>-ocimene, methyl benzoate, eucalyptol, and sensors W2S, W1S, W3S, and W2W as critical elements for differentiating fragrance quality at various stages of cut lilies. Furthermore, PLS regression analysis revealed a significant positive correlation between the W2W sensor and key VOCs, including (<em>E</em>)-<em>β</em>-ocimene, <em>α</em>-terpinene<em>, α</em>-phellandrene, terpinolene, <em>β</em>-myrcene, <em>allo</em>-ocimene, creosol, and cosmen. Additionally, W3S and W6S sensors showed strong positive correlations with methyl tiglate. These sensors can serve as potential markers for the corresponding compound concentrations, indicating that monitoring these sensor responses can rapidly detect the important fragrance compounds in cut lilies. The results provide a potential method for the efficient and rapid detection of postharvest floral scent quality in cut lilies. Utilizing the real-time detection advantage of the electronic nose can enhance the efficiency of postharvest fragrance quality detection in cut lilies by several orders of magnitude.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113378"},"PeriodicalIF":6.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143092608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and metabolome analyses reveal the effects of brassinolide treatment on the ripening and flavor development of postharvest kiwifruit","authors":"Yaming Yang,&nbsp;Ming Chen,&nbsp;Zhaoting Li,&nbsp;Qinggang Zhu,&nbsp;Xiaolin Ren,&nbsp;Yanrong Lv,&nbsp;Yuduan Ding","doi":"10.1016/j.postharvbio.2024.113380","DOIUrl":"10.1016/j.postharvbio.2024.113380","url":null,"abstract":"<div><div>Brassinosteroids (BRs), a class of steroid phytohormones, are involved in regulating fruit ripening processes. However, the molecular mechanisms by which this hormone controls fruit ripening in kiwifruit remain unclear. Herein, 2,4-epibrassinolide (EBR) treatment delayed the decline in fruit firmness, thereby delaying the ripening of kiwifruit. Transcriptomic and metabolomic analyses revealed that the EBR treatment influenced the sugar metabolism pathways and the fruit flavor development. We found that the <em>AdERF2</em> and <em>AdBAM3.5</em> expressions were downregulated by EBR and that AdERF2 could activate the promoter activity of <em>AdBAM3.5</em>. AdERF2 was localized to the nucleus, and transient overexpression of <em>AdERF2</em> promoted starch degradation. These results indicate that BRs inhibited the activation of <em>AdBAM3.5</em> expression by AdERF2, slowing down starch degradation and promoting the accumulation of soluble sugars, which may influence ripe kiwifruit taste and overall flavor perception.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113380"},"PeriodicalIF":6.4,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143092604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}