Postharvest Biology and Technology最新文献_第8页

Debaryomyces nepalensis enhances suberin deposition in citrus fruit wound by facilitating the synthesis of cinnamic acid and its derivatives 尼泊尔德巴利酵母菌通过促进肉桂酸及其衍生物的合成，增强柑橘果实伤口中的单宁沉积

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2024-10-10 DOI: 10.1016/j.postharvbio.2024.113244

Jian Huang , Qi Chen , Ou Chen , Xiaoquan Gao , Rong Zhu , Rui Huang , Shixiang Yao , Lanhua Yi , Kaifang Zeng

{"title":"Debaryomyces nepalensis enhances suberin deposition in citrus fruit wound by facilitating the synthesis of cinnamic acid and its derivatives","authors":"Jian Huang , Qi Chen , Ou Chen , Xiaoquan Gao , Rong Zhu , Rui Huang , Shixiang Yao , Lanhua Yi , Kaifang Zeng","doi":"10.1016/j.postharvbio.2024.113244","DOIUrl":"10.1016/j.postharvbio.2024.113244","url":null,"abstract":"<div><div>Mechanical damage to citrus fruit is inevitable when picking, transporting, storing, and marketing. Previous studies have shown that citrus fruit have the ability to wound healing. Suberin is the signature substance for wound healing. This study investigates the effect of <em>Debaryomyces nepalensis</em> treatment on promoting citrus fruit wound healing and its mechanism. The effect of <em>D. nepalensis</em> was assessed through the examination of changes in suberin content at the wounds of citrus fruit treated with <em>D. nepalensis</em> using an ortho-fluorescent microscopy, as well as the occurrence of green mold in fruit treated with <em>D. nepalensis</em>. Wound samples treated with <em>D. nepalensis</em> for 3 d and those healed naturally over the same period were analyzed through transcriptome and metabolome evaluations. Results showed significant suberin accumulation in treated wounds compared to natural healing. <em>D. nepalensis</em> notably improved the healing process and reduced green mold disease incidence and lesion diameter. Additionally, <em>D. nepalensis</em> activated the phenylpropane biosynthesis pathway, which led to the upregulation of 34 genes, including <em>COMT</em>, <em>HCT</em>, <em>CAD</em>, and <em>PAL</em>. Consequently, this activation also resulted in a significant increase in the content of phenolic acids, including cinnamic acid and its derivatives, caffeic acid and chlorogenic acid. Moreover, the exogenous application of these phenolic acids further promoted wound healing and reduced green mold incidence. In conclusion, <em>D. nepalensis</em> activates the phenylpropane biosynthesis pathway, enhances the expression of crucial genes like <em>COMT</em>, and increases the content of substances such as caffeic acid to facilitate wound healing in citrus fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113244"},"PeriodicalIF":6.4,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silencing Sly-miR159 accelerated preharvest ripening and delayed postharvest senescence of tomato fruit 沉默 Sly-miR159 可加速番茄果实的采前成熟和采后衰老

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2024-10-07 DOI: 10.1016/j.postharvbio.2024.113243

Wenhui Duan , Shuangshuang Shan , Xiaoyun Ye , Shen Ni , Qing Wang , Zhengke Zhang , Lanhuan Meng , Lisha Zhu , Jiali Yang , Hongmiao Song , Xiangbin Xu

{"title":"Silencing Sly-miR159 accelerated preharvest ripening and delayed postharvest senescence of tomato fruit","authors":"Wenhui Duan , Shuangshuang Shan , Xiaoyun Ye , Shen Ni , Qing Wang , Zhengke Zhang , Lanhuan Meng , Lisha Zhu , Jiali Yang , Hongmiao Song , Xiangbin Xu","doi":"10.1016/j.postharvbio.2024.113243","DOIUrl":"10.1016/j.postharvbio.2024.113243","url":null,"abstract":"<div><div>The miR159 is an ancient and highly conserved microRNA (miRNA), which is frequently expressed in various tissues and developmental phases, as well as participated extensively in plant growth by regulating multiple target genes. In the present study, the tomato lines of silencing-miR159 by short tandem target mimic (STTM-miR159) and overexpression-miR159 (OE-miR159) were obtained by Agrobacterium-mediated transformation. The results showed that the OE-miR159 caused male sterility and induced parthenocarpy by decreasing the expression level of <em>SlMYB120</em>. The STTM-miR159 accelerated the preharvest ripening of tomato fruit by increasing the expression level of <em>SlACS8</em>. The STTM-miR159 reduced the accumulation of carotenoids and delayed the postharvest senescence of tomato fruit by affecting the ethylene signaling pathway. This study expanded the cognition of function of miR159-<em>SlACS8</em>/<em>SlMYB120</em> module in fertility traits, ripening and senescence of tomato fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113243"},"PeriodicalIF":6.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlled atmosphere storage enhances Korean melon shelf life and quality: A comparative metabolic analysis with reefer container export 恒温贮藏可提高韩国甜瓜的货架期和质量：与冷藏集装箱出口的代谢比较分析

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2024-10-05 DOI: 10.1016/j.postharvbio.2024.113238

Woochul Ju , Ji-Hyun Lee , Eunsu Do , Da-yeong Ko , Eungu Jee , Min-Sun Chang , Hyang Lan Eum , Haejo Yang , Kang-Mo Ku

{"title":"Controlled atmosphere storage enhances Korean melon shelf life and quality: A comparative metabolic analysis with reefer container export","authors":"Woochul Ju , Ji-Hyun Lee , Eunsu Do , Da-yeong Ko , Eungu Jee , Min-Sun Chang , Hyang Lan Eum , Haejo Yang , Kang-Mo Ku","doi":"10.1016/j.postharvbio.2024.113238","DOIUrl":"10.1016/j.postharvbio.2024.113238","url":null,"abstract":"<div><div>Korean melons are renowned for their taste and nutritional qualities but face challenges such as chilling injury and browning during post-harvest storage, limiting their export potential. To address these issues, we compared melons stored in Controlled Atmosphere (CA) containers to those in Reefer Container (CON) under simulated export conditions. This study examines the effects of CA storage on the quality, metabolite changes, and volatile organic compounds (VOC) of Korean melons (<em>Cucumis melo var. makuwa</em>). CA-stored Korean melons showed increased concentrations of cutin monomers, suggesting reduced browning due to the expression of cutin-related genes. VOCs analysis revealed distinctive patterns in both Korean melon skin and juice, with specific compounds elevated in CA storage. The breakdown of linolenic acid into (<em>E</em>,<em>Z</em>)-2,6-nonadienal was reduced by approximately 30 % under CA storage, likely due to reduced oxygen availability. Twister-assisted VOCs analysis highlighted aroma differences, indicating potential effects on sensory perception. Solubility changes showed a 15 % increase in glyceric acid and a 20 % decrease in oxalate levels under CA storage, suggesting improved flavor and reduced harmful compounds. The freshness model based on VOCs indicated that CA-treated melons remained fresh approximately 4 d longer than control melons. In conclusion, CA storage improves the quality, freshness, and nutritional profile of Korean melons and offers valuable insights for refining post-harvest storage practices. Further research is warranted to explore the long-term effects of CA storage on other fruits and vegetables.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113238"},"PeriodicalIF":6.4,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Key factors uncovered by transcriptomic analysis in the regulation of glutamic acid repressing the browning of fresh-cut potatoes 转录组分析揭示谷氨酸抑制鲜切马铃薯褐变调控的关键因素

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2024-10-05 DOI: 10.1016/j.postharvbio.2024.113242

Qinghua Guan , Wenhui Li , Mei Dai , Jin Qiao , Bin Wu , Zheng Zhang , Jingying Shi , Zunyang Song

{"title":"Key factors uncovered by transcriptomic analysis in the regulation of glutamic acid repressing the browning of fresh-cut potatoes","authors":"Qinghua Guan , Wenhui Li , Mei Dai , Jin Qiao , Bin Wu , Zheng Zhang , Jingying Shi , Zunyang Song","doi":"10.1016/j.postharvbio.2024.113242","DOIUrl":"10.1016/j.postharvbio.2024.113242","url":null,"abstract":"<div><div>Enzymatic browning is a major problem that seriously impacts the quality of fresh-cut potatoes. Our previous study found that glutamic acid (Glu) treatment could repress the discoloration of fresh-cut potatoes, but the molecular mechanism was still unknown. Herein, it was found that the content of total phenolic, H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>.-</sup> and the activities of PPO, POD and PAL were repressed, but the activities of CAT, APX, SOD and GPX were improved by Glu treatment. Moreover, transcriptomic analysis showed that an abundant number of differentially expressed genes (DEGs) were selected from fresh-cut potatoes browning between Glu and control groups. A comprehensive functional enrichment analysis showed that the metabolic pathways of glutathione metabolism, flavonoid biosynthesis, phenylalanine metabolism, phenylpropanoid biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction were markedly enriched, which transcriptional levels were markedly altered by Glu treatment. RT-qPCR confirmed the results of RNA-Seq that Glu repressed the transcription of <em>StPPO2</em>, <em>StPPO3</em>, <em>StPPO7</em>, and <em>StERF-BR1-like</em>. More importantly, as a nuclear protein, StERF-BR1-like activated the transcription of <em>StPPO2</em> by directly binding with its promoter. Overall, these data indicate that Glu could repress the browning of fresh-cut potatoes by regulating the pathways mentioned above, and StERF-BR1-like may involve this process by stimulating the expression of <em>StPPO2</em>.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113242"},"PeriodicalIF":6.4,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of linalool in managing Alternaria alternata infection and delaying black mold rot in goji berry 芳樟醇在控制枸杞交替孢霉感染和延缓黑霉菌腐烂方面的作用

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2024-10-03 DOI: 10.1016/j.postharvbio.2024.113240

Huaiyu Zhang , Yueli Zhou , Yue Yang , Lunaike Zhao , Hongbo Li , Lijie Long , Junjie Wang , Xuyan Wu , Zhao-Jun Wei

{"title":"The role of linalool in managing Alternaria alternata infection and delaying black mold rot in goji berry","authors":"Huaiyu Zhang , Yueli Zhou , Yue Yang , Lunaike Zhao , Hongbo Li , Lijie Long , Junjie Wang , Xuyan Wu , Zhao-Jun Wei","doi":"10.1016/j.postharvbio.2024.113240","DOIUrl":"10.1016/j.postharvbio.2024.113240","url":null,"abstract":"<div><div><em>Alternaria alternata</em>, a common fungal pathogen causes black mold in postharvest goji fruit. To explore the <em>in vitro</em> and <em>in vivo</em> impacts of linalool on the fungal infection, we treated <em>A. alternata</em> and infected goji fruit with varying linalool concentrations (0, 0.15, 0.45, and 1.35 mL L<sup>−1</sup>). Linalool repressed hypha growth, spore yield and germination, and germ tube length of <em>A. alternata</em>. Furthermore, we observed abnormal spore and mycelium morphology in the treated samples using optical, scanning electron, and transmission electron microscopy. Linalool reduced lesion diameter and disease incidence in goji fruit. Moreover, transcriptomic analysis of pathogen revealed that linalool inhibited nitrogen metabolism, altered chitin and <em>β</em>-1,3-glucan metabolisms, downregulated the key gene expression related to ergosterol synthesis and sulfur and glutathione metabolisms, regulated the enzymatic antioxidant system, and impacted the proportion of unsaturated fatty acids. Furthermore, linalool damaged the fungal cell wall integrity by inducing alkaline phosphatase and chitinase activities and <em>β</em>-1,3-glucan content and repressing <em>β</em>-1,3-glucanase and chitin synthase activities and chitin levels. Linalool elevated superoxide dismutase and catalase activities and declined peroxidase and glutathione reductase activities and glutathione content in <em>A. alternata</em>, leading to increased H<sub>2</sub>O<sub>2</sub> levels and ROS stress. Our data exhibited the promising antifungal effects of linalool with a future application in management of the postharvest rot of goji fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113240"},"PeriodicalIF":6.4,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tomato Synaptotagmin F accelerates fruit ripening, shortens fruit shelf-life and increases susceptibility to Penicillium expansum 番茄 Synaptotagmin F 可加速果实成熟、缩短果实货架期并增加对扩张青霉的敏感性

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2024-10-03 DOI: 10.1016/j.postharvbio.2024.113235

Ziyu Wang , Min Miao , Dongxin Tao , Xiaofeng Tang , Feng Zhang , Hongtao Wang , Xue Fang , Yongsheng Liu , Pengpeng Zheng

{"title":"Tomato Synaptotagmin F accelerates fruit ripening, shortens fruit shelf-life and increases susceptibility to Penicillium expansum","authors":"Ziyu Wang , Min Miao , Dongxin Tao , Xiaofeng Tang , Feng Zhang , Hongtao Wang , Xue Fang , Yongsheng Liu , Pengpeng Zheng","doi":"10.1016/j.postharvbio.2024.113235","DOIUrl":"10.1016/j.postharvbio.2024.113235","url":null,"abstract":"<div><div>Synaptotagmins (SYTs), initially identified as calcium sensors for regulating synaptic vesicle exocytosis and endocytosis in mammalian neurons, play crucial role in biotic and abiotic stresses in plants. However, the function of SYTs in fruit ripening is unclear. In this study, a tomato Synaptotagmins gene, <em>SlSYTF</em>, was found to accelerate tomato fruit ripening. <em>SlSYTF</em> encodes an endoplasmic reticulum localized protein whose transcription is continuously enhanced during fruit ripening. Overexpression <em>SlSYTF</em> in tomato resulted in accelerated ripening progress, increased carotenoid content as well as decreased the firmness of tomato fruit, whereas the mutant <em>slsytf-c</em> exhibited the opposite phenotype. Importantly, SlSYTF could increase ethylene production by activating the expression of ethylene synthesis genes and prompt cell wall degradation by increasing pectinase and cellulase activities. Nevertheless, the accelerated cell wall degradation and thinned cuticle due to SlSYTF results in reduced shelf life and pathogen resistance. Collectively, we revealed a new <em>SYTs</em> gene that plays a dual role in tomato fruit ripening and pathogen response. This finding may shed new light on the relationship between maturation and immunity.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113235"},"PeriodicalIF":6.4,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CsbHLH6 positively regulates abscisic acid-mediated citrus fruit coloration during postharvest storage CsbHLH6 在采后贮藏期间对脱落酸介导的柑橘果实着色具有正向调节作用

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2024-10-03 DOI: 10.1016/j.postharvbio.2024.113229

Quan Sun , Zhengchen He , Yingzi Zhang , Di Feng , Ranran Wei , Lijun Chai , Wenwu Guo , Juan Xu , Yunjiang Cheng , Qiang Xu , Junli Ye , Xiuxin Deng

{"title":"CsbHLH6 positively regulates abscisic acid-mediated citrus fruit coloration during postharvest storage","authors":"Quan Sun , Zhengchen He , Yingzi Zhang , Di Feng , Ranran Wei , Lijun Chai , Wenwu Guo , Juan Xu , Yunjiang Cheng , Qiang Xu , Junli Ye , Xiuxin Deng","doi":"10.1016/j.postharvbio.2024.113229","DOIUrl":"10.1016/j.postharvbio.2024.113229","url":null,"abstract":"<div><div>As is well known, postharvest abscisic acid (ABA) treatment of citrus fruits can significantly enhance fruit coloration by promoting the accumulation of carotenoids, however, the underlying molecular mechanism is still unclear. In this study, we further confirmed the effect of ABA treatment in promoting citrus fruit coloration by activating the expression of 4 key carotenoid metabolism genes (<em>CsPSY</em>, <em>CsLCYB</em>, <em>CsHYD</em>, and <em>CsNCED2</em>) during postharvest storage. A novel basic helix-loop-helix (bHLH) transcription factor, CsbHLH6, was identified using the promoters of 3 key ABA-induced carotenoid metabolism genes (<em>CsLCYB</em>, <em>CsHYD</em>, and <em>CsNCED2</em>) by yeast one-hybrid screening. Overexpression of <em>CsbHLH6</em> enhanced carotenoid accumulation in citrus fruits and calli. Interference with <em>CsbHLH6</em> expression in citrus calli and fruits indicated that <em>CsbHLH6</em> was essential for ABA-induced carotenoid biosynthesis and fruit coloration. As a nucleus-localized transcriptional activator, CsbHLH6 directly bound to the promoters of 6 key carotenoid metabolism genes (<em>CsPSY</em>, <em>CsLCYB</em>, <em>CsLCYE</em>, <em>CsHYD</em>, <em>CsZEP</em>, and <em>CsNCED2</em>), subsequently activating their expression <em>in vitro</em> and <em>in vivo</em>. Furthermore, ABA signaling significantly raised <em>CsbHLH6</em> promoter activity, thus enhancing the regulation of carotenoid accumulation by <em>CsbHLH6</em>. In summary, our study reveals the molecular mechanism by which CsbHLH6 regulates ABA-induced citrus fruit coloration during postharvest storage, which is crucial for improving the postharvest appearance quality of citrus fruits.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113229"},"PeriodicalIF":6.4,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The use of Trichoderma species for the biocontrol of postharvest fungal decay in fruits and vegetables: Challenges and opportunities 利用毛霉菌种对水果和蔬菜采后真菌腐烂进行生物防治：挑战与机遇

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2024-10-01 DOI: 10.1016/j.postharvbio.2024.113236

Xiaojiao Li , Qinhong Liao , Shixian Zeng , Yong Wang , Jia Liu

{"title":"The use of Trichoderma species for the biocontrol of postharvest fungal decay in fruits and vegetables: Challenges and opportunities","authors":"Xiaojiao Li , Qinhong Liao , Shixian Zeng , Yong Wang , Jia Liu","doi":"10.1016/j.postharvbio.2024.113236","DOIUrl":"10.1016/j.postharvbio.2024.113236","url":null,"abstract":"<div><div>Ensuring the stability of the global supply of fruits and vegetables and maintaining the quality and nutritional value of fruits and vegetables during storage and marketing are critical problems that need to be continually addressed. However, postharvest losses due to decay and senescence pose a major threat to the supply of high-quality fruits and vegetables. In fact, postharvest losses caused by decay pathogens can account for 20–25 % of postharvest losses. In recent years, new methods to control postharvest diseases of fruits and vegetables have been explored that are considered safer and more ecofriendly than the use of synthetic, chemical fungicides. In this regard, the use of <em>Trichoderma</em> species as biocontrol agents of both preharvest and postharvest diseases has been the subject of considerable research. Their use for the prevention of postharvest diseases is especially noteworthy as <em>Trichoderma</em> has been shown to be non-toxic and non-harmful to humans and the environment. In this review we provide an overview of the advances that have been made in the use of <em>Trichoderma</em> isolates for controlling postharvest diseases of fruits and vegetables. The review provides information on the identification of <em>Trichoderma</em> species with biocontrol potential, their mode of action, and the challenges and opportunities that exist for the commercial application of <em>Trichoderma</em> in the postharvest environment. Future research topics that need to be addressed on the use of <em>Trichoderma</em> are also presented.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113236"},"PeriodicalIF":6.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular polygalacturonase, β-1,4-glucanase and β-xylosidase in Geotrichum citri-aurrantii positively progressed the sour rot incidence in satsuma mandarin fruit 柑橘地衣菌中的胞外聚半乳糖醛酸酶、β-1,4-葡聚糖酶和β-木糖苷酶对沙糖桔果实酸腐病的发生有积极的促进作用

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2024-09-28 DOI: 10.1016/j.postharvbio.2024.113233

Haowen Ai, Yonghua Zhang, Okwong Oketch Reymick, Xuemeng Shen, Wen Liu, Nengguo Tao, Lu Li

{"title":"Extracellular polygalacturonase, β-1,4-glucanase and β-xylosidase in Geotrichum citri-aurrantii positively progressed the sour rot incidence in satsuma mandarin fruit","authors":"Haowen Ai, Yonghua Zhang, Okwong Oketch Reymick, Xuemeng Shen, Wen Liu, Nengguo Tao, Lu Li","doi":"10.1016/j.postharvbio.2024.113233","DOIUrl":"10.1016/j.postharvbio.2024.113233","url":null,"abstract":"<div><div>Gaining an understanding of the characteristics that contribute to the infectiousness of pathogens is essential for devising new strategies to control fungal infection. This study aims to identify and clarify the role of cell wall degrading enzymes (CWDEs) secreted by the <em>Geotrichum citri-aurantii</em> pathogen in the development of sour rot disease in Satsuma mandarin fruit. Results from <em>in vivo</em> experiments showed that CWDEs were secreted during arthrospore germination. Genomic sequencing and liquid chromatography-tandem mass spectrometry results unveiled the presence of polygalacturonase (PG), β-1,4-glucanase, α-glucosidase, xyloglucan:xyloglucosyl transferase, and β-xylosidase. Enzyme activity and gene expression analyses exposed the indispensable roles of PG and β-1,4-glucanase in the initial phase, and β-xylosidase during the acute phase of infection. After infection, we observed that the quantities of cellulose, pectin, and hemicellulose declined continuously, while the soluble pectin content rose steadily. Molecular docking analysis demonstrated that the diversity of CWDE binding to substrates complicated the <em>G. citri-aurantii</em> infection process. Furthermore, we determined the importance of asparagine and aspartic acid in PG-substrate interaction and glycine in β-xylosidase-ligand interaction. Taken together, our findings suggest that PG is a crucial target for blocking the development of sour rot in citrus fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113233"},"PeriodicalIF":6.4,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pickering emulsions stabilized by chitosan nanoparticles and loaded with clove essential oil as a green biopesticide: A case study on controlling fungal growth in oranges 由壳聚糖纳米颗粒稳定并添加丁香精油的皮克林乳剂作为一种绿色生物农药：控制柑橘中真菌生长的案例研究

IF 6.4 1区农林科学

Postharvest Biology and Technology Pub Date : 2024-09-27 DOI: 10.1016/j.postharvbio.2024.113234

Maryam Mottaki , Seid Mahdi Jafari , Seyyed Morteza Hosseini , Alireza Sadeghi , Fatemeh Heydari-Delfard

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