Terpinen-4-ol enhances blueberry resistance to Alternaria via VcMYB36-mediated lignin biosynthesis

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-09-08 DOI:10.1016/j.postharvbio.2025.113892

Yuxuan Li , Jiaqi Wang , Xunfei Zhu , Runan Zhao , Meihui Wen , Bingxin Sun , Yunhe Zhang , Yufeng Xu , Xuerui Yan

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Abstract

Blueberry Alternaria fruit rot (AFR), caused by Alternaria tenuissima, is one of the most serious postharvest diseases affecting blueberry quality in northern China. Terpinen-4-ol (T4O), a natural monoterpene with a mild aroma, shows antifungal potential; however, its effectiveness and underlying mechanism on controlling AFR remain unclear. In this study, blueberry fruit were fumigated with 0.4 μL mL⁻¹ T4O and inoculated with A. tenuissima. The incidence during storage was first monitored. Transcriptome sequencing was then employed to identify differentially expressed genes (DEGs) followed by validation. Finally, the yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), and transient expression assays were conducted to further elucidate the regulatory mechanism of transcription factors on key genes. The results showed that T4O treatment reduced disease incidence by 40 % at 5 d and increased fruit firmness by 27 % compared to the control. Transcriptome analysis indicated that T4O application induced the expression of lignin biosynthesis-related genes (VcCCR1, VcCAD, and VcPOD) in the phenylpropanoid pathway. Among them, VcCCR1 exhibited the highest upregulation (Log₂FC = 8.96) and was positively correlated with lignin content, suggesting its potential role in lignin accumulation in inoculated blueberries. In addition, Y1H and EMSA confirmed that the transcription factor VcMYB36 specifically bond to the CAACCA cis-element in the VcCCR1 promoter. Transient expression assays further indicated that VcMYB36 positively regulated VcCCR1 expression, which may contribute to lignin accumulation and improved resistance of blueberry fruit to AFR.

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通过vcmyb36介导的木质素生物合成，松油烯-4-醇增强蓝莓对交替病菌的抗性

蓝莓霉霉病（Alternaria fruit rot， AFR）是中国北方地区影响蓝莓品质最严重的采后病害之一。油松烯-4-醇（t40）是一种香气温和的天然单萜，具有抗真菌的潜力；然而，其控制AFR的有效性和潜在机制尚不清楚。本研究以0.4 μL mL−1 t40o熏蒸蓝莓果实，接种tenuissima。首先监测储存期间的发病率。然后采用转录组测序鉴定差异表达基因（DEGs），并进行验证。最后，通过酵母单杂交（Y1H）、电泳迁移转移（EMSA）和瞬时表达实验进一步阐明转录因子对关键基因的调控机制。结果表明，与对照相比，t40o处理在第5天降低了40 %的发病率，增加了27 %的果实硬度。转录组分析表明，t40o诱导了苯丙素途径中木质素生物合成相关基因（VcCCR1、VcCAD和VcPOD）的表达。其中，VcCCR1表达上调幅度最大（Log2FC = 8.96），且与木质素含量呈正相关，提示其可能参与接种蓝莓木质素积累。此外，Y1H和EMSA证实转录因子VcMYB36特异性结合VcCCR1启动子中的CAACCA顺式元件。瞬时表达实验进一步表明，VcMYB36正调控VcCCR1的表达，这可能与蓝莓果实木质素积累和抗AFR能力的提高有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.