Postharvest Biology and Technology最新文献

{"title":"A nonlinear classification method for online sorting of navel orange SSC based on near-infrared spectroscopy and data augmentation","authors":"Shaohui Yu ,&nbsp;Jing Liu","doi":"10.1016/j.postharvbio.2025.113990","DOIUrl":"10.1016/j.postharvbio.2025.113990","url":null,"abstract":"<div><div>As a non-invasive detection method, near-infrared spectroscopy (NIR) has demonstrated significant potential for application in assessing fruit quality and sorting. However, during the online fruit sorting process, multiple factors affect the sorting accuracy. To address the challenges of limited sample size, heterogeneous quality, and the intricate nonlinear relationship between detection indices and spectral data in online sorting, this paper presents a data augmentation approach for the online sorting of navel oranges based on the soluble solids content (SSC), which integrates error rate and probability mass weighting. Firstly, cluster analysis was performed on the SSC, and the R² statistic and the elbow rule were introduced to determine the optimal number of clusters. The training set and test set were divided using the Monte Carlo random sampling method. Subsequently, the training set was augmented by incorporating spectral data that had undergone moving average smoothing, thereby forming an enhanced sample set. Furthermore, the probability mass and error rate of training set samples were integrated to formulate the sample weight coefficient. At last, the augmented training set was employed to establish a classification model via a three-layer neural network. Multiple experimental results showed that this method significantly improves classification performance for online sorting data, and the classification accuracy exceeds 90 %.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113990"},"PeriodicalIF":6.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coronatine promotes post-harvest softening in ‘Gui Qi’ mango (Mangifera indica L.) through regulation of β-galactosidase and ethylene biosynthesis-related genes","authors":"Ziqing Mi ,&nbsp;Bilal A. Rather ,&nbsp;Hanqing Cong ,&nbsp;Yibo Bai ,&nbsp;Rongxiang Wang ,&nbsp;Bo Zheng ,&nbsp;Xuefei Jiang ,&nbsp;Pingyin Guan ,&nbsp;Fei Qiao","doi":"10.1016/j.postharvbio.2025.113989","DOIUrl":"10.1016/j.postharvbio.2025.113989","url":null,"abstract":"<div><div>Fruit softening is a key physiological event during ripening that directly influences texture, consumer acceptance, and early market supply. In ‘Gui Qi’ mango (<em>Mangifera indica</em> L.), timely and uniform softening is essential for commercial value and shelf-life management. While ethylene is well established as the primary regulator of ripening and softening in climacteric fruits, the role of coronatine (COR) in this process has not yet been investigated in ‘Gui Qi’ mango. In this study we investigated the effect of exogenous COR on mango fruit ripening and softening. It was observed that COR treatment had no effect on sugar and acid metabolism, but significantly reduced the fruit firmness and malondialdehyde (MDA) content, and increased peroxidase (POD) and catalase (CAT) activities. In addition, we have identified 24 mango β-galactosidase (<em>Miβ-GAL)</em>-encoding genes by transcriptome analysis. The results of relative gene expression showed that COR postharvest-treatment induced the expression of <em>Miβ-GAL2</em>, which exhibits a significant correlation with the ethylene level and its biosynthetic encoding genes such as S-adenosylmethionine synthetase (SAMS), 1-aminocyclopropane-1-carboxylate synthase (ACS), and aminocyclopropanecarboxylate oxidase (ACO) and synergically promote fruit softening. Therefore, treatment with COR modulates the expression of several <em>Miβ-GAL genes</em>, leading to increased β-GAL activity and a faster postharvest softening process in ‘Gui Qi’ mango.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113989"},"PeriodicalIF":6.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of antioxidant activity and postharvest quality of fresh-cut artichoke by dipping in flavonoid-rich bergamot juice","authors":"Palumbo Michela ,&nbsp;Quintieri Laura ,&nbsp;Bartella Lucia ,&nbsp;Di Donna Leonardo ,&nbsp;Santoro Ilaria ,&nbsp;Luparelli Anna ,&nbsp;Caputo Leonardo ,&nbsp;Pace Bernardo ,&nbsp;Cozzolino Rosaria ,&nbsp;Matarazzo Cristina ,&nbsp;Cefola Maria","doi":"10.1016/j.postharvbio.2025.113986","DOIUrl":"10.1016/j.postharvbio.2025.113986","url":null,"abstract":"<div><div>Artichoke (<em>Cynara cardunculus</em> var. <em>scolymus</em>) is a vegetable highly perishable when it is processed as fresh-cut products due to the related preparation steps which can physically alter it and favour a major risk of contamination and degradation. In this work, for the first time, fresh-cut artichoke heads were dipped in a bergamot juice extract (BE 0.05 % w/v) containing flavonoids with antimicrobial and antioxidant properties (neoeriocitrin, naringin, neohesperidin, peripolin, melitidin and brutieridin), and stored for 9 d at 5 °C. Compared to the controls, and ethanol (1 %) dipped samples, BE-dipped fresh-cut artichokes, showed improved sensory attributes, lower respiration rate and a reduction of <em>Pseudomona</em>s spoilers by 1 log CFU g⁻¹ up to 6 d of storage. Moreover, an increase in antioxidant activity was also observed in BE-dipped samples throughout the storage, putatively related to the added flavonoids (naringin, melitidin, brutieridin), which were retained up to 6 d of storage. The dipping in BE also preserved the organoleptic properties of these samples; indeed, volatile organic compounds (VOCs) profiles of artichoke dipped in BE solution showed a higher content of “green leaves” volatiles. Among these, 1-hexanol, 2-hexen-1-ol, <em>cis</em>-3-hexen-1-ol, hexanal, cis-2-hexenal, were correlated with the sensory attributes of freshness. In conclusion, in this work, a sustainable and innovative strategy based on the dipping of fresh-cut artichokes in BE proposed for the first time has showed to improve the antioxidant value and the shelf-life of the samples, preserving their sensory attributes.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113986"},"PeriodicalIF":6.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan inhibits the growth of Penicillium digitatum and Penicillium italicum and protects oranges from green and blue mold during postharvest","authors":"Simone Piancatelli ,&nbsp;Adrian O. Sbodio ,&nbsp;Thomas Bruno Michelon ,&nbsp;Muyun Tsen ,&nbsp;Mary Carmen Carranza-Rodriguez ,&nbsp;Elia Gutierrez-Baeza ,&nbsp;Gianfranco Romanazzi ,&nbsp;Barbara Blanco-Ulate","doi":"10.1016/j.postharvbio.2025.113955","DOIUrl":"10.1016/j.postharvbio.2025.113955","url":null,"abstract":"<div><div>Postharvest losses in oranges caused by <em>Penicillium</em> spp. infections are a major concern for the citrus industry worldwide. In this study, we examined the antimicrobial properties of chitosan against various <em>Penicillium</em> strains, including some resistant to fungicides. We also assessed the effectiveness of chitosan as a protective coating for navel oranges to prevent infections. Chitosan at 0.5 % concentration in media inhibited <em>Penicillium digitatum</em> growth, while <em>Penicillium italicum</em> required 1 % chitosan for complete inhibition. We then coated oranges by dipping them with chitosan, either alone or combined with commercial wax, to compare their effectiveness in controlling <em>Penicillium</em> infections against a conventional treatment with fungicide and wax. Additional controls included oranges treated with wax only or left untreated. Oranges were later contact-inoculated with <em>P. digitatum</em> and <em>P. italicum</em> strains under favorable conditions for disease development. Oranges coated with chitosan 1 %, 2 %, and 1 % + wax had a significantly lower cumulative incidence of infections than those treated with wax only or left untreated. Chitosan-based edible coatings also outperformed fungicide and wax treatments. Chitosan 2 % reduced the incidence of decay caused by fungicide-resistant <em>P. digitatum</em> and <em>P. italicum</em> strains by 68 % and 76 %, compared to conventionally treated oranges. Additionally, combining chitosan with wax extended the shelf life and enhanced the marketability of the oranges compared to all other treatments. These findings suggest chitosan as a viable complement or alternative to synthetic fungicides for managing green and blue mold on navel oranges in the postharvest supply chain.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113955"},"PeriodicalIF":6.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DcCAMTA6-DcCML8 promotes GABA accumulation in fresh-cut carrots via transcriptional activation of polyamine metabolism genes","authors":"Jinglin Zhang ,&nbsp;Wanli You ,&nbsp;Xueyin Ru ,&nbsp;Feng Xu ,&nbsp;Zhengguo Wu ,&nbsp;Peng Jin ,&nbsp;Yonghua Zheng ,&nbsp;Shifeng Cao","doi":"10.1016/j.postharvbio.2025.113985","DOIUrl":"10.1016/j.postharvbio.2025.113985","url":null,"abstract":"<div><div>Polyamine (PAs) degradation serves as a key pathway for γ-aminobutyric acid (GABA) synthesis in plants. While calcium chloride (CaCl₂) treatment is known to induce GABA accumulation in fresh-cut carrots, the underlying molecular mechanism regulating PAs metabolism remains unclear. In this study, CaCl₂ treatment increased calmodulin (CaM), GABA, [Ca²⁺ ]_cyt, ornithine, arginine and histidine contents in fresh-cut carrots. It concurrently enhanced the degradation of PAs by upregulating the transcript levels and enzyme activities of arginine decarboxylase (ADC), polyamine oxidase (PAO), and diamine oxidase (DAO). Conversely, treatment with the CaM antagonist trifluoperazine resulted in lower GABA content, which was achieved by reducing the activities and expression levels of ADC and PAO. Furthermore, CaCl₂ treatment induced the expression of <em>DcCAMTA3, DcCAMTA4, DcCAMTA6</em>, and <em>DcCML8</em>. Moreover, DcCAMTA3, DcCAMTA4, and DcCAMTA6 up-regulated the transcription of the <em>DcADC</em> and <em>DcPAO1</em> genes, thereby enhancing GABA biosynthesis. Further analysis revealed that DcCAMTA6 protein directly binds to the CGTG cis-element in the promoters of <em>DcADC</em> and <em>DcPAO1</em> and cooperates with DcCML8 to activate their expression. Therefore, our findings indicate that CaCl₂ treatment enhances the conversion of PAs to GABA through the DcCAMTA6-DcCML8-mediated upregulation of <em>DcADC</em> and <em>DcPAO1</em> expression. This finding expands the theoretical basis for understanding GABA accumulation induced by CaCl₂ treatment in fresh-cut carrots.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113985"},"PeriodicalIF":6.8,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of the sprout-suppressive effects of rosemary essential oil on potato tubers","authors":"Shutong Wu ,&nbsp;Xiangbin Xu ,&nbsp;Di Wu ,&nbsp;Peng Zhang ,&nbsp;Guozheng Qin ,&nbsp;Baodong Wei ,&nbsp;Jiangkuo Li","doi":"10.1016/j.postharvbio.2025.113991","DOIUrl":"10.1016/j.postharvbio.2025.113991","url":null,"abstract":"<div><div>Postharvest sprouting of potato tubers compromises food safety and causes economic losses. Although synthetic sprout suppressants have been widely used, concerns over their safety and environmental impact have led to increasing restrictions. Therefore, safe and sustainable plant-derived alternatives are needed, yet the physiological and molecular mechanisms by which natural compounds inhibit sprouting remain poorly understood. This study investigated rosemary essential oil (REO) as a natural sprouting suppressant. REO fumigation delayed sprout emergence, reduced weight loss, and maintained nutritional quality over 100 d of storage. REO suppressed α-amylase activity and modulated sucrose metabolism by downregulating sucrose phosphate synthase (SPS) while enhancing sucrose synthase (SS) and invertase (INV) activity. To elucidate the molecular basis of REO-induced inhibition, transcriptomic analysis was conducted on tubers stored for 40 d. Notably, the Control 40 d vs. REO 40 d comparison exhibited 655 differentially expressed genes (DEGs), enriched in zeatin biosynthesis, plant hormone signal transduction, and starch-sucrose metabolism pathways. REO upregulated glycosyltransferase genes (<em>UGT73C3</em> and <em>CISZOG</em>) involved in cytokinin inactivation, activated cytokinin repressors (<em>ARR4</em> and <em>ARR9</em>), and modulated auxin, ethylene, and brassinosteroid signaling through regulation of <em>AUX22D</em>, <em>GH3.5</em>, <em>ERF1</em>, and <em>BKI1</em>. Concurrently, REO upregulated genes associated with sucrose degradation and glycoside hydrolysis (<em>INV</em>, <em>SS</em>, <em>SS7</em>, <em>BoGH3B</em>, <em>bglB40/46/11</em>, and <em>EG6</em>), while downregulated genes involved in sucrose synthesis (<em>SPS2</em>) and starch degradation (<em>amyA3</em>). These findings suggest that REO suppresses sprouting by regulating gene expression in plant hormone and starch-sucrose metabolism pathways, highlighting its potential as an effective and safe plant-derived sprouting inhibitor.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113991"},"PeriodicalIF":6.8,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The MADS-box transcription factor AaRLMA serves a crucial function in sustaining the integrity of cell wall, orchestrating secondary metabolites biosynthesis, and modulating pathogenicity in Alternaria alternata","authors":"Rong Li,&nbsp;Yiyang Liu,&nbsp;Xiwang Wang,&nbsp;Pengfei Sun,&nbsp;Meiqi Gao,&nbsp;Yanxia Zhang,&nbsp;Yang Bi,&nbsp;Yongcai Li","doi":"10.1016/j.postharvbio.2025.113993","DOIUrl":"10.1016/j.postharvbio.2025.113993","url":null,"abstract":"<div><div>RLMA, a transcription factor belonging to the MADS-box family, serves a pivotal function within the fungi cell wall integrity (CWI) pathway. This signaling cascade is responsible for sensing and responding to cellular wall stress signals, and is intimately linked to key aspects of fungal biology, including growth, development, and pathogenicity. Nevertheless, the precise regulatory functions of RLMA in <em>Alternaria alternata</em> have not been fully elucidated. In this study, we successfully cloned and performed functional characterization of AaRLMA. Remarkably, the knockout of <em>AaRLMA</em> led to a decrease in colony diameter, biomass production, and sporulation. Infection ability test revealed that Δ<em>AaRLMA</em> exhibited weakened cellophane penetration, along with reduced spore germination and appressorium-like formation. Additionally, Δ<em>AaRLMA</em> mutant exhibited heightened susceptibility to calcofluor white (CFW), concurrent with the downregulation of key genes involved in cell wall synthesis. Remarkably, an elevation in the content of secondary metabolite DHN (1,8-dihydroxynaphthalene) melanin was observed in the Δ<em>AaRLMA</em>, parallel to the downregulation of major melanin biosynthesis related genes. Furthermore, the pathogenicity of Δ<em>AaRLMA</em> was diminished under both wound-inoculated and non-wound-inoculated conditions, with significant decreases observed in the contents of several mycotoxins, and down-regulated of related genes expression. Additionally, enzymatic activity of cell wall degrading enzymes (CWDEs) were substantially lower in the Δ<em>AaRLMA.</em> This study offers fresh perspectives on the regulatory mechanisms underpinning the biological control of <em>A. alternata</em>.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113993"},"PeriodicalIF":6.8,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145322774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arachidonic acid enhances gray mold resistance in postharvest tomato via modulating phenylpropanoid biosynthesis, plant-pathogen interaction, and MAPK signaling pathway","authors":"Pengcheng Gao ,&nbsp;Xiao Sun ,&nbsp;Tingting Zhang ,&nbsp;Geying Liang ,&nbsp;Xujie Dong ,&nbsp;Jiqing Peng ,&nbsp;Chaozhen Zeng ,&nbsp;Zhixiang Liu","doi":"10.1016/j.postharvbio.2025.113981","DOIUrl":"10.1016/j.postharvbio.2025.113981","url":null,"abstract":"<div><div>Cherry tomatoes (<em>Solanum lycopersicum</em> var. <em>cerasiforme</em>), an economically important crop, are highly susceptible to gray mold caused by <em>Botrytis cinerea</em> during postharvest storage, resulting in deterioration of fruit quality. As a polyunsaturated fatty acid, arachidonic acid (ARA) has been shown to act as an elicitor to induce plant defense responses against pathogens. In the present work, we explored the inducing effects and mechanisms of ARA on gray mold resistance through physiological and transcriptomic analyses. Exogenous ARA increased the activities of defense-related enzymes, such as polyphenol oxidase, peroxidase, catalase, phenylalanine ammonia-lyase, and ascorbate peroxidase, as well as significantly suppressed malondialdehyde accumulation in tomato. Transcriptomic analyses revealed that ARA treatment highlighted coordinated changes in the key pathways, including phenylpropanoid biosynthesis, plant-pathogen interaction, and MAPK signaling pathway, resulting in enhanced resistance against <em>B. cinerea</em> in tomato. The up-regulation of calcium signaling-related genes (<em>CDPK</em> and <em>CNGCs</em>) induced early reactive oxygen species burst and hypersensitive response. MEKK1-MKK1/2-MPK4 and MEKK1-MKK4/5-MPK3/6 cascade in the MAPK signaling pathway regulated transcription factors (WRKY25/33, WRKY22/29, Pti5, and Pti6), which in turn induced defense-related genes (<em>PR1</em> and <em>ChiB</em>). Concurrently, the upregulation of key genes (<em>4CL</em>, <em>PAL</em>, <em>POD</em>, <em>CCoAOMT</em>, and <em>CYP98A3</em>) in the phenylpropanoid metabolic pathway promoted lignin deposition and the accumulation of antimicrobial compounds, establishing a dual-layered physical-chemical defense barrier. This study provides new insights into the resistance of cherry tomato against <em>B. cinerea</em> induced by ARA.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113981"},"PeriodicalIF":6.8,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145270932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low temperature delays longan pulp breakdown through DlMYB108-DlPME17 transcriptional regulation","authors":"Qiu-yi Ye ,&nbsp;Zhong-qi Fan ,&nbsp;Wei Shan ,&nbsp;Jian-fei Kuang ,&nbsp;Jian-ye Chen ,&nbsp;Wang-jin Lu ,&nbsp;Hui Wang ,&nbsp;Hetong Lin ,&nbsp;Wei Wei","doi":"10.1016/j.postharvbio.2025.113987","DOIUrl":"10.1016/j.postharvbio.2025.113987","url":null,"abstract":"<div><div>Postharvest pulp degradation significantly constrains the shelf life and commercial value of longan fruits. This investigation revealed a temperature-responsive transcriptional network centered on the DlMYB108–DlPME17 regulatory module that controls longan pulp deterioration. Physiological analyses demonstrated that 10 °C effectively delayed pulp degradation by maintaining cell wall integrity and inhibiting hydrolase activities compared with 25 °C. Transcriptomic profiling identified 9372 differentially expressed genes, with cell wall metabolism being significantly enriched. Among these, <em>DlPME17</em> (Dil.04g002100.1) exhibited substantial upregulation correlating with degradation progression. Molecular characterization identified DlMYB108 as a nuclear-localized transcriptional activator demonstrating low-temperature inhibitory expression. DlMYB108 directly binds to the <em>DlPME17</em> promoter and activates its expression. These findings establish DlMYB108 as a critical regulator of pectin disassembly through transcriptional control of <em>DlPME17</em>, offering insights into the molecular mechanisms underlying postharvest longan quality deterioration.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113987"},"PeriodicalIF":6.8,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145270401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrazine-2-carboxylic acid maintains pummelo quality by modulating ROS homeostasis through the CgWRKY31–CgPOD52 module","authors":"Mingzhe Yang ,&nbsp;Yanxue Guo ,&nbsp;Wei Shan ,&nbsp;Jian-fei Kuang ,&nbsp;Wang-jin Lu ,&nbsp;Jian-ye Chen ,&nbsp;Lubin Zhang ,&nbsp;Haihua Luo ,&nbsp;Wei Wei","doi":"10.1016/j.postharvbio.2025.113971","DOIUrl":"10.1016/j.postharvbio.2025.113971","url":null,"abstract":"<div><div>Reactive oxygen species (ROS) accumulation during postharvest senescence significantly compromises the commercial quality of ‘Sanhongmiyou’ pummelo (<em>Citrus grandis</em>) fruit. Transcription factors in post-harvest pomelo fruits have not been the subject of sufficient study, therefore this study aims to further analyse the molecular mechanisms of transcription factor-mediated reactive oxygen species (ROS) metabolism and lignification. This study demonstrates that pyrazine-2-carboxylic acid (PCA) treatment effectively inhibits deterioration of pummelo pulp quality. Physiological analyses indicate that PCA-treated fruits exhibited reduced lignin content and malondialdehyde accumulation compared with controls, while maintaining elevated antioxidant enzyme activities (superoxide dismutase [SOD], catalase [CAT], and peroxidase [POD]) and enhanced ascorbate-glutathione cycle efficiency. Transcriptomic analysis identified a key WRKY transcription factor (CgWRKY31) negatively correlated with ROS. Subcellular localization revealed that CgWRKY31 functions as a nuclear-localization protein. Electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter (DLR) assay demonstrated that CgWRKY31 directly activates the POD gene <em>CgPOD52</em> through specific binding to its W-box element, enhancing ROS scavenging capacity. These findings establish a regulatory mechanism where PCA treatment maintains postharvest quality by CgWRKY31-mediated antioxidant enhancement, providing a promising strategy for citrus fruit preservation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113971"},"PeriodicalIF":6.8,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145270402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}