Postharvest Biology and Technology最新文献

{"title":"Electron-beam generated X-ray irradiation treatment alleviates fruit-body softening of harvested Hericium erinaceus by regulating metabolisms of membrane lipid and cell wall","authors":"Yuanyuan Zhong ,&nbsp;Yuan Cui ,&nbsp;Jiangtao Yu ,&nbsp;Junqing Bai ,&nbsp;Huaide Xu ,&nbsp;Mei Li","doi":"10.1016/j.postharvbio.2024.113317","DOIUrl":"10.1016/j.postharvbio.2024.113317","url":null,"abstract":"<div><div>Fresh <em>Hericium erinaceus</em> is susceptible to softening after harvest, 1.0 kGy electron-beam generated X-ray (EBGX) irradiation could alleviate this phenomenon. To clarify the possible mechanism of EBGX regulated the softening of <em>H. erinaceus</em>, we explored its effects on membrane lipid and cell wall metabolism. Results showed that 1.0 kGy irradiation enhanced cell membrane stability, as evidenced by higher levels of phosphatidic acid, phosphatidylcholine, phosphatidylinositol, and the ratio of unsaturated fatty acids to saturated fatty acids (U/S), whereas lower levels of ROS, lipoxygenase, phospholipase D, and lipase activities compared to the control. More importantly, 1.0 kGy-treated samples exhibited a minimum of chitinase (4.12 U mg⁻¹), cellulase (16.34 U mg⁻¹), β-glucanase (13.28 U mg⁻¹), and PAL (10.17 U mg⁻¹) activities at 9d, but retained higher chitin, cellulose, β-glucan, and lignin contents, thus suppressed the disassembly of cell wall structure. Utilizing comparative transcriptomic data, the differentially expressed genes (DEGs) from starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism, and lipid degradation metabolism were related to softening in <em>H. erinaceus</em> with control or irradiation treatments. Additionally, the relative abundance of DEGs-related transcripts like <em>HeLOX</em>, <em>HePLD</em>, <em>HeLPS</em>, <em>HeCHI</em>, <em>HeCEL</em>, <em>HeGLU1</em>, <em>HeGLU2</em>, and <em>HePAL</em> were prominently downregulated in the irradiation treatment. Overall, 1.0 kGy EBGX irradiation could retard the postharvest softening process of <em>H. erinaceus</em> by regulating the gene expression of membrane lipids and cell wall metabolic pathways.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"220 ","pages":"Article 113317"},"PeriodicalIF":6.4,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methyl jasmonate attenuates chilling injury of prune fruit by maintaining ROS homeostasis and regulating GABA metabolism and energy status","authors":"Yating Zhao ,&nbsp;Yingjie Wu ,&nbsp;Xuan Zhang ,&nbsp;Xuan Zhu ,&nbsp;Yuanyuan Hou ,&nbsp;Jianye Chen ,&nbsp;Kuanbo Cui ,&nbsp;Xuewen Li ,&nbsp;Wenxin Wu","doi":"10.1016/j.postharvbio.2024.113303","DOIUrl":"10.1016/j.postharvbio.2024.113303","url":null,"abstract":"<div><div>Cold storage is an important means to prolong the storage life of postharvest fruits. However, prunes are prone to chilling injury (CI) at low temperatures, which in turn reduces their marketability. In this study, methyl jasmonate (MeJA) effectively inhibited the occurrence of CI, and reduced internal browning index and electrolyte leakage of prune fruit under low temperature (1 ± 1 °C), with the most significant inhibitory effect observed at the concentration of 10 μM. Also, MeJA alleviated the overproduction of reactive oxygen species (ROS) by promoting the activity of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase) and the expression level of their coding genes, thereby maintaining the integrity of the mitochondrial structure of prune fruit. Moreover, MeJA maintained higher content of γ-aminobutyric acid (GABA) through stimulating glutamate decarboxylase activity and retained higher energy levels by promoting the activity of succinate dehydrogenase, cytochrome C oxidase, H⁺-ATPase, Ca²⁺-ATPase, GABA transaminase, and succinic semialdehyde dehydrogenase and expression level of the corresponding gene of prune fruit. Our findings not only shed light on the inhibitory effect of MeJA on CI of prune fruit from the perspective of reducing ROS-induced oxidative damage to maintain mitochondrial structure, activating GABA shunt, and maintaining energy supply but also provide means of alleviating CI of prune fruit during postharvest storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"220 ","pages":"Article 113303"},"PeriodicalIF":6.4,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing aflatoxin B1 detection in peanut kernels through deep modular combination optimization algorithm: A deep learning approach to quality evaluation of postharvest nuts","authors":"Zhen Guo ,&nbsp;Haifang Wang ,&nbsp;Haowei Dong ,&nbsp;Lianming Xia ,&nbsp;Ibrahim A. Darwish ,&nbsp;Yemin Guo ,&nbsp;Xia Sun","doi":"10.1016/j.postharvbio.2024.113293","DOIUrl":"10.1016/j.postharvbio.2024.113293","url":null,"abstract":"<div><div>Aflatoxin B₁ (AFB₁) is considered one of the most potent natural carcinogens. Quantitative detection of AFB₁ is essential for quality evaluation of postharvest nuts. In this study, a deep modular combination optimization (DMCO) algorithm was proposed to detect the content of AFB₁ in peanut kernels contaminated with <em>Aspergillus flavus</em>. The DMCO algorithm constituted a groundbreaking approach in the realm of deep learning for hyperspectral imaging analysis which meticulously selected and modularized existing deep learning models. It was characterized by the flexibility of combining these modules in serial configurations, parallel configurations or more complex configurations. This innovative architecture facilitated the capture of complex features, leading to improved predictive performance over single-module models. A performance-based selection mechanism was included in DMCO algorithm, which determined the most effective model architectures from a multitude of permutations. The optimal module combination reached a coefficient of determination for validation of 0.879, with root mean square error for validation and mean absolute error for validation recorded at 1.269 and 0.945, respectively. The DMCO algorithm successfully leverages deep learning to enhance the accuracy of AFB₁ detection in peanut kernels, showing its potential as a powerful tool to assess safety and quality for postharvest nuts.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"220 ","pages":"Article 113293"},"PeriodicalIF":6.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene co-expression network analysis of the transcriptome identifies cold-resistant hub genes related to carbohydrate metabolism in peach fruit","authors":"Xianzhu Deng ,&nbsp;Yingying Wei ,&nbsp;Yi Chen ,&nbsp;Phebe Ding ,&nbsp;Feng Xu ,&nbsp;Xingfeng Shao","doi":"10.1016/j.postharvbio.2024.113300","DOIUrl":"10.1016/j.postharvbio.2024.113300","url":null,"abstract":"<div><div>In order to explore the key factors involved in the regulation of chilling injury formation in carbohydrate metabolism of peach fruit, we systematically analyzed the response of peach fruit to cold stress from physiological and transcriptomic perspectives. Cold stress in peach elevated malondialdehyde, impaired membranes, reduced sucrose via invertase-mediated hydrolysis to fructose/glucose, and may activate pentose phosphate pathway while inhibiting glycolysis pathways for metabolic maintenance and energy conservation, as suggested by phosphofructokinase and glucose-6-phosphate dehydrogenase activity shifts. Transcriptome and weighted gene co-expression network analysis revealed a series of genes respond to low-temperature stress in starch and sucrose metabolism pathway, including vacuolar invertases gene (<em>PpVIN</em>), hexokinase gene (<em>PpHXK</em>), trehalose-phosphate synthase gene (<em>PpTPS</em>), trehalose-phosphate phosphatase gene (<em>PpTPP</em>)<em>,</em> and fructokinase gene (<em>PpFRK</em>), of these hub genes have been reported to resist to cold stress. In addition, by constructing co-expression network, several transcription factors (TFs) were involved in regulating hub genes related to carbohydrate metabolism in peach fruit, such as ZAT12, bZIP23, bZIP53, CBF5, and others. These TFs may serve as key regulators of the transcriptional network, modulating gene expression related to carbohydrate metabolism in response to cold stress.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"220 ","pages":"Article 113300"},"PeriodicalIF":6.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detecting water-soaked disorder and reddish-pulp disorder in peach fruit using bio-speckle","authors":"Sae Tashiro ,&nbsp;Akane Yoshimura ,&nbsp;Takuya Hashimoto ,&nbsp;Fumina Tanaka ,&nbsp;Piotr Mariusz Pieczywek ,&nbsp;Artur Zdunek ,&nbsp;Fumihiko Tanaka","doi":"10.1016/j.postharvbio.2024.113297","DOIUrl":"10.1016/j.postharvbio.2024.113297","url":null,"abstract":"<div><div>This study addresses the challenges in nondestructive identifying diseases, particularly water-soaked and reddish-pulp disorders, in peaches during storage and transport. Existing technologies have struggled to detect these diseases during this period, leading to potential food loss and consumer distrust. Biospeckle has emerged as a promising discriminator of the internal state of the fruit by utilizing laser-induced scattered light patterns. Pigment interference is minimized by employing lasers with wavelengths of 532 and 650 nm. This study focuses on the ability of biospeckle to distinguish between healthy and diseased fruit based on characteristic values, specifically the Fujii index and cumulative amplitude (Cum. amp.) at 2–3, 3–4, 4–5, and 6–7 Hz. The <em>t</em>-test results demonstrated significant differences in these values, particularly for water-soaked and reddish-pulp disorders. Biospeckle outperforms other non-destructive methods by identifying the symptoms pre-storage. These results indicate that Cum. amp. at 3–4, 4–5, and 6–7 Hz may be more useful in identifying water-soaked fruit than the Fujii index and Cum. amp. at 2–3 Hz. Red lasers are more effective in detecting reddish-pulp disorders than green lasers, which are hindered by pigment absorption. This finding highlights the potential of biospeckle in precise symptom identification, which is crucial for ensuring food quality and consumer confidence.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"220 ","pages":"Article 113297"},"PeriodicalIF":6.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The PpWRKY22-PpWRKY70 regulatory module enhances resistance to Monilinia fructicola by regulating the gamma-aminobutyric acid shunt in peach fruit","authors":"Wenhui Li,&nbsp;Mei Dai,&nbsp;Xiaorui Wang,&nbsp;Yingying Shi,&nbsp;Zhimin Wang,&nbsp;Dapeng Li,&nbsp;Jingying Shi,&nbsp;Zunyang Song","doi":"10.1016/j.postharvbio.2024.113306","DOIUrl":"10.1016/j.postharvbio.2024.113306","url":null,"abstract":"<div><div>Peach fruit is susceptible to infection of <em>Monilinia fructicola</em> leading to brown rot. Our previous study demonstrated that nitric oxide (NO) enhanced fruit resistance to <em>M. fructicola</em> by mediating the gamma-aminobutyric acid (GABA) shunt. Herein, two genes, <em>PpWRKY22</em> and <em>PpWRKY70</em>, were identified whose expression was closely associated with the resistance of peach fruit against <em>M. fructicola</em> and induced by NO treatment. Notably, PpWRKY22 and PpWRKY70 activate the transcription of three GABA shunt-related genes (<em>PpSSADH</em>, <em>PpGABA-T</em> and <em>PpGAD4</em>) by directly binding to their promoters. Protein interaction assays revealed that PpWRKY22 interacts with PpWRKY70, and their protein complex enhances the promoter activities of <em>PpSSADH</em>, <em>PpGABA-T</em> and <em>PpGAD4</em>. Additionally, transient overexpression of <em>PpWRKY22</em> and <em>PpWRKY70</em> in peach fruit increases resistance to <em>M. fructicola</em> via up-regulating the transcription level of <em>PpSSADH</em>, <em>PpGABA-T</em> and <em>PpGAD4</em>. Overall, this study uncovers a novel model in which the interaction between PpWRKYs proteins actively enhances peach fruit resistance to <em>M. fructicola</em> by promoting the expression of GABA shunt-related genes.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"220 ","pages":"Article 113306"},"PeriodicalIF":6.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A combined analysis of transcriptome and proteome reveals the regulation mechanism of alginate oligosaccharides on alleviating energy deficit in postharvest strawberry","authors":"Wenxia Wang ,&nbsp;Santosh Kumar Bose ,&nbsp;Xiaochen Jia ,&nbsp;Prianka Howlader ,&nbsp;Heng Yin","doi":"10.1016/j.postharvbio.2024.113302","DOIUrl":"10.1016/j.postharvbio.2024.113302","url":null,"abstract":"<div><div>Strawberry exhibits a very short shelf life due to its rapid softening and decay, resulting in postharvest loss. Our previous study showed that AOS, an eco-friendly nontoxic natural product, plays a role in preserving the freshness of strawberries by regulating ABA content and its signaling pathway. Considering the critical role that energy deficit plays in the senescence process of fruits, coupled with the potential influence of plant hormones on energy metabolism. In this study, the molecular mechanism of strawberry postharvest senescence in response to AOS treatment was further explored from the perspective of energy metabolism regulation. This was achieved through a two-layered approach, integrating the direct determination of energy-related substances with a comprehensive joint analysis of transcriptome and proteome data. AOS treatment significantly delayed the loss of ATP, ADP, and AMP contents compared to control fruit, implying that maintaining cell energy is a crucial factor in AOS postponing senescence in strawberry. Subsequently, the mechanisms were further revealed by transcriptome and proteome analysis. Overall, 255 DEGs were screened from the AOS group, mainly related to carbohydrate and energy metabolism, defense, plant hormone, and secondary metabolism. Additionally, A total of 227 proteins were identified that were differentially expressed in the AOS group, and the majority were related to carbohydrate and energy metabolism (85 proteins). Two omics data implied that AOS could effectively regulate the energy metabolism system to maintain the cellular energy level of harvested strawberry during storage, ultimately leading to longer shelf life. Therefore, our findings provide comprehensive information regarding the molecular mechanism underlying the postharvest storage of strawberry treated with AOS. From our results, it can be concluded that AOS postharvest treatment is very useful for keeping fruit quality and extending shelf life by maintaining a higher energy level.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"220 ","pages":"Article 113302"},"PeriodicalIF":6.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preharvest 1-methylcyclopropene and aminoethoxyvinylglycine treatment effects on ‘NY2’ (RubyFrost®) apple fruit quality and postharvest watercore dissipation at different temperatures","authors":"Burak E. Algul ,&nbsp;Yosef Al Shoffe ,&nbsp;DoSu Park ,&nbsp;Lailiang Cheng ,&nbsp;Christopher B. Watkins","doi":"10.1016/j.postharvbio.2024.113301","DOIUrl":"10.1016/j.postharvbio.2024.113301","url":null,"abstract":"<div><div>Recent studies have found that dissipation of watercore in apple fruit after harvest can be enhanced by preharvest application of 1-methylcyclopropene (1-MCP). In this study, the effects of plant growth regulators (PGRs) on watercore dissipation have been extended to the use of aminoethoxyvinylglycine (AVG) and the effects of storage at 3 °C, 10 °C, and 20 °C over 30 d. Preharvest 1-methylcyclopropene (1-MCP) and aminoethoxyvinylglycine (AVG) treatments were applied one week before harvest to 'NY2' (RubyFrost®), a cultivar that is prone to development of a stress-associated type of watercore during fruit maturation. Fruit were harvested twice (H1 and H2), 11 d apart. 1-MCP and AVG treatments had more pronounced effects on maturity at H2, with higher I_AD values and flesh firmness, respectively, compared with those of fruit harvested at H1. During storage, 1-MCP and AVG treated fruit had lower ethylene production and respiration rates than untreated fruit at H1, but ethylene production in 1-MCP treated fruit increased on day 17 at H2, reaching levels similar to untreated fruit, while remaining low in AVG-treated fruit. IECs were lower in fruit stored at 3 °C than at 10 °C or 20 C. Both treatments maintained low IECs, but more persistently in AVG-treated fruit. Flesh firmness and titratable acidity were higher, and greasiness was lower, in AVG and 1-MCP treated fruit compared with untreated fruit. The PGR treatments had no effect on watercore incidence at H1 as incidence was low in all treated fruit. At H2, 1-MCP reduced watercore incidence at harvest compared with untreated and AVG-treated fruit, which had similar incidences. Watercore dissipation was enhanced by AVG, being more rapid with higher storage temperature. The mechanism by which PGRs can increase watercore dissipation is unknown, but they might alter sorbitol transporter activity and/or cell membrane stability for sorbitol uptake from the intercellular space into the parenchyma cells. Nevertheless, the use of PGRs may be a useful way to decrease the incidences of flesh browning disorders associated with high watercore incidence in fruit at the time of harvest.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"220 ","pages":"Article 113301"},"PeriodicalIF":6.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142658990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics analysis reveals the regulatory role of PsERF3 in anthocyanin biosynthesis of ‘Taoxingli’ plum fruit in response to MeSA treatment","authors":"Lichuan Lou,&nbsp;Xiaoli Hu,&nbsp;Jiao Cheng,&nbsp;Yuan Cheng,&nbsp;Mengyao Yin,&nbsp;Chen Huan,&nbsp;Xiaolin Zheng,&nbsp;Shuling Shen","doi":"10.1016/j.postharvbio.2024.113298","DOIUrl":"10.1016/j.postharvbio.2024.113298","url":null,"abstract":"<div><div>Mature plum fruit (<em>Prunus salicina</em> cv. Taoxingli) exhibits an attractive red appearance and significant nutritional value due to anthocyanin accumulation. Some ‘Taoxingli’ plums are harvested before reaching full ripeness to prevent fruit cracking caused by hot and rainy weather. At this stage, plum fruit has not reached its optimal quality. In this study, methyl salicylate (MeSA) treatment promoted anthocyanin accumulation in earlier-harvested ‘Taoxingli’ plum fruit during postharvest. Transcriptomic techniques were employed to unravel the regulatory mechanisms. A weighted gene co-expression network analysis (WGCNA) was constructed based on RNA-seq data, and 22 co-expression modules were constructed. In the specific module, seven synthetic genes were identified. Thus, a transcription factor (TF)-target gene regulatory network related to anthocyanin biosynthesis was built. Yeast one-hybrid assays and electrophoretic mobility shift assay (EMSA) indicated that PsERF3 could directly bind to the promoter of <em>PsANS</em> via RAA motif. Transient over-expression of PsERF3 accelerated anthocyanin biosynthesis in apple through inducing structural genes expression. This work offers fresh perspectives on the regulatory mechanisms of anthocyanin accumulation in ‘Taoxingli’ plum fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"220 ","pages":"Article 113298"},"PeriodicalIF":6.4,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics and metabolomics profiling revealed the adaptive mechanism of Penicillium digitatum under modified atmosphere packaging-simulated gas stress","authors":"Zhengli Wang ,&nbsp;Shanshan Hao ,&nbsp;Huihui Xu,&nbsp;Nana Ji,&nbsp;Yanyin Guo,&nbsp;Muhammad Asim","doi":"10.1016/j.postharvbio.2024.113290","DOIUrl":"10.1016/j.postharvbio.2024.113290","url":null,"abstract":"<div><div>Modified atmosphere packaging (MAP) is an effective method to preserve fruit and vegetables. However, pathogen inhibition can be challenging due to their adaptive capacity. This study used combined transcriptome and metabolome analyses to elucidate the adaptive mechanism of <em>Penicillium digitatum</em> (<em>P. digitatum</em>) under MAP-simulated gas stress based on its carbohydrates and energy metabolism. The colony diameter of <em>P. digitatum</em> in the MAP treatment was comparable to that in the Air treatment and significantly larger than that in the controlled atmosphere (CA) treatment. Transcriptome and metabolome analyses revealed that MAP treatment facilitated the degradation of 1,3-β glucan, glycogen, and sucrose to glucose, ensuring a sustained glucose supply for the glycolysis (EMP) and pentose phosphate (PP) pathways. Upregulation of related genes in the MAP treatment accelerated the EMP and PP pathways and promoted oxidative glucose degradation. MAP-simulated gas stress augmented <em>P. digitatum</em>’s adaptive response by promoting the EMP, alcoholic fermentation, PP, tricarboxylic acid (TCA) cycle, and oxidative phosphorylation (OXPHOS) pathways and increasing energy production. Based on reverse thinking, this study suggests a potential control strategy for <em>P. digitatum</em> contamination during MAP storage of fruit and vegetables.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113290"},"PeriodicalIF":6.4,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}