鲜切辣椒（Capsicum annuum L.）受肠杆菌感染后细胞壁多糖和 ROS 代谢变化的启示

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-03-13 DOI:10.1016/j.postharvbio.2025.113499

Zudi Li , Xiaoyan Zhao , Dan Wang , Pan Wang , Shuang Zhao , Hangjun Chen , Yanchao Han , Wenting Zhao

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Insights into the changes in cell wall polysaccharides and ROS metabolism of fresh-cut chili pepper (Capsicum annuum L.) infected by Enterobacter cloacae

Enterobacter cloacae, a typical spoilage bacterium, causes severe textural softening of freshly cut chili pepper. This study investigated the mechanisms of E. cloacae-induced textural softening involving cell wall polysaccharide disassembly and reactive oxygen species (ROS) production. It was found that E. cloacae-inoculated samples had lower firmness and greater weight loss than the control group. Meanwhile, E. cloacae inoculation promoted cell wall decomposition; cell wall polysaccharide disassembly, such as cellulose and Na₂CO₃-soluble pectin; and enhanced cell wall-degrading enzyme (CWDE) activity (polygalacturonase, pectin methylase, pectin lyase, β-galactosidase, α-L-arabinofuranosidase, cellulase, and xyloglucan endotransglycosylase). Furthermore, high H₂O₂ content, low GSH content, and reduced activities of superoxide dismutase, catalase, and ascorbate peroxidase were found in the E. cloacae-inoculated group during late storage. Overall, E. cloacae-induced textural softening of fresh-cut chili peppers resulted from cell-wall polysaccharide degradation due to increased CWDE activity, ROS accumulation, and reduced ROS-scavenging capacity.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.