Postharvest Biology and Technology最新文献

{"title":"Postharvest calcium chloride dips. An effective strategy to reduce Botrytis blight severity and increase petal strength in cut roses.","authors":"Melissa Muñoz ,&nbsp;Logan E. Behnke ,&nbsp;William C. Bridges ,&nbsp;Guido Schnabel ,&nbsp;James E. Faust","doi":"10.1016/j.postharvbio.2024.113292","DOIUrl":"10.1016/j.postharvbio.2024.113292","url":null,"abstract":"<div><div>Botrytis blight in cut-flower roses is a devastating disease caused by <em>Botrytis cinerea.</em> Calcium (Ca) applications have shown great potential as alternative disease management strategies for different crops. This research aimed to evaluate the effect of preharvest calcium sprays and postharvest calcium dips on rose flower susceptibility to Botrytis blight, petal tissue calcium concentration, and petal strength. Calcium chloride spray applications (0, 500, 1000, and 1500 mg^.L⁻¹of Ca) during greenhouse production and postharvest calcium chloride dips (0, 1000, and 2000 mg^.L⁻¹ of Ca) were evaluated and compared with commercial controls, including hydrogen peroxide, captan, and pydiflumethophen + fludioxonil. Calcium chloride sprays during production reduced Botrytis blight severity at 500 and 1000 mg^.L⁻¹ of Ca; however, no concentration of calcium chloride spray solution increased calcium in the petal tissue. Postharvest calcium chloride dip applications at 1000 and 2000 mg^.L⁻¹ of Ca effectively reduced Botrytis blight compared to the untreated control. Calcium chloride dip applications at 2000 mg^.L⁻¹ of Ca were effective for increasing the calcium concentration in the epidermis and mesophyll of the petal tissue in comparison to the 0 mg^.L⁻¹ treatment and resulted in increased petal strength and reduced Botrytis severity in comparison to captan and hydrogen peroxide treatments. These results show that postharvest calcium chloride dip applications are more effective than spray applications during production in improving calcium concentration and petal strength on roses and result in a reduction of Botrytis blight severity greater than captan and hydrogen peroxide commercial treatments. This underscores the importance of calcium chloride applications as an alternative management approach for Botrytis blight on roses.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113292"},"PeriodicalIF":6.4,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salvia miltiorrhiza-derived carbon dots delay postharvest senescence through repressing BrTCP9-mediated reactive oxygen species metabolism in flowering Chinese cabbage","authors":"Lingqi Yue ,&nbsp;Hongyi Qin ,&nbsp;Lifeng Kuang ,&nbsp;Xuewu Duan ,&nbsp;Dengjin Kang ,&nbsp;Yunyan Kang ,&nbsp;Min Zhong ,&nbsp;Xian Yang","doi":"10.1016/j.postharvbio.2024.113296","DOIUrl":"10.1016/j.postharvbio.2024.113296","url":null,"abstract":"<div><div><em>Salvia miltiorrhiza</em>-derived carbon dots (<em>Sm</em>CDs), as a novel type of nanomaterials, play an important role in delaying senescence and maintaining quality in postharvest flowering Chinese cabbage. However, understanding the underlying molecular mechanisms of <em>Sm</em>CDs involved in delaying senescence is still unclear and requires further studies. In this work, the expression of <em>BrTCP9</em> among the 16 <em>BrTCPs</em> members was found to correlate with the morphological phenotype observed during leaf aging. Silencing <em>BrTCP9</em> hindered the degradation of chlorophyll, inhibited the expressions of chlorophyll decomposition-associated genes (<em>BrNYE1</em>, <em>BrNYC1</em>, <em>BrNOL</em>, and <em>BrPPH</em>) and respiratory burst oxidase homologs genes (<em>BrRbohA</em> and <em>BrRbohD</em>), increased the activities of peroxidase (POD) and superoxide dismutase (SOD), slowed down the production of reactive oxygen species (ROS), and enhanced <em>Fv/Fm</em> value, which ultimately delayed leaf senescence. Interestingly, the changing trends of chlorophyll content, chlorophyll degradation-related gene expression, ROS production, <em>Fv/Fm</em> value, and leaf phenotype after silencing <em>BrRbohA</em> were similar to those of silencing <em>BrTCP9</em>. Notably, <em>Sm</em>CDs could downregulate the transcriptions of <em>BrTCP9</em> and <em>BrRbohA</em>, attenuate the production of ROS, and delay leaf senescence. Furthermore, BrTCP9 could directly bind to the <em>GGTCCCA</em> motif in the promoter region of <em>BrRbohA</em> and activate its transcript. Our findings demonstrated that BrTCP9 served as a positive regulator of leaf senescence in flowering Chinese cabbage. In contrast, <em>Sm</em>CDs effectively delayed leaf senescence by suppressing BrTCP9-mediated ROS metabolism to reduce the accumulation of ROS.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113296"},"PeriodicalIF":6.4,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of harvest maturity and storage conditions on tomato quality: A comprehensive experimental and modeling study","authors":"Steven Duret ,&nbsp;Christophe Aubert ,&nbsp;Sophie Annibal ,&nbsp;Evelyne Derens-Bertheau ,&nbsp;Valentine Cottet ,&nbsp;Michel Jost ,&nbsp;Guillaume Chalot ,&nbsp;Denis Flick ,&nbsp;Jean Moureh ,&nbsp;Onrawee Laguerre ,&nbsp;Valérie Mérendet ,&nbsp;Elsa Desnoues","doi":"10.1016/j.postharvbio.2024.113286","DOIUrl":"10.1016/j.postharvbio.2024.113286","url":null,"abstract":"<div><div>The study was applied to fresh tomatoes (<em>Solanum lycopersicum</em>, Marbone variety). First, a field survey was conducted to obtain statistical data on the temperature and duration (minimum, mode, maximum) at different stages in the supply chain. Then, the physicochemical, phytochemical and volatile compounds were measured at harvest and on five different days (3, 7, 11, 14 and 21 days) for two maturity stages at harvest (green-orange and light red) and under three storage temperatures (3 °C, 8 °C and 15 °C). These data were used to evaluate the combined impact of harvest maturity, time and storage temperature on the evolution of the different compounds. From the same data, quality models were developed based on the Arrhenius equation to describe the tomato quality evolution under different storage temperatures for (<em>Z</em>)-3 hexenal, total sum of acids, lycopene and chlorogenic acid. These compounds were chosen because of their relation to commercial and nutritional qualities. The model results were validated using additional data from a different study conducted on one constant temperature profile and two dynamic time-temperature profiles as in a supply chain. Finally, data from the field survey and the quality models were combined in a Monte Carlo process (10⁵ product items) to simulate the variability of time-temperature profiles from the grower to the sales outlet and its impact on the quality attributes. For two maturity stages at harvest, the impact of the supply chain on the quality attributes of tomatoes at the sales outlet was also analyzed. Simulation results show that the maturity stage at harvest significantly impacts the final lycopene content and the total sum of acids in tomatoes at the sales outlet, with fruit harvested at the light red stage containing higher lycopene levels (and a lower hue angle) and a lower total sum of acids in comparison with fruit harvested at the green-orange stage. However, at the sales outlet, the concentrations of chlorogenic acid and (<em>Z</em>)-3-hexenal in fruit harvested at the green-orange and light red stages are similar. This information would be useful for fruit and vegetable stakeholders for time-temperature management of tomato fruit throughout the supply chain in order to optimally preserve the main quality attributes.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113286"},"PeriodicalIF":6.4,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CmERFⅠ-5 and CmERFⅤ-2 affect ethylene synthesis and sucrose accumulation in postharvest oriental melon fruit at low temperature","authors":"Fan Yang ,&nbsp;Ge Gao ,&nbsp;Cheng Wang ,&nbsp;Jingyue Guan ,&nbsp;Junqin Chen ,&nbsp;Hongyan Qi","doi":"10.1016/j.postharvbio.2024.113295","DOIUrl":"10.1016/j.postharvbio.2024.113295","url":null,"abstract":"<div><div>Postharvest low-temperature storage crucially affects ethylene-dependent fruit ripening and the sucrose accumulation of climacteric melon fruit; however, the molecular mechanism by which ethylene affects sugar content at low temperature remains unclear. Here, ‘High Sucrose’ (‘HS’) melon fruit were harvested at the initial stage of ethylene release (30 d after anthesis, DAA) and treated at different temperature (25 ℃, 10 ℃, and 4 ℃). The results revealed that both low temperature treatments had a lower sucrose content and sucrose percentage while also having a higher starch content, hexose level, vacuolar invertase (VINV) activity, and <em>CmVINV2</em> expression in the melon fruit. Moreover, the ethylene production and <em>CmACO1</em> (<em>ACC oxidase 1</em>) expression were lower at low temperature. Both low-temperature treatments positively and negatively regulated the expression of the ethylene response factors <em>CmERFV-2</em> and <em>CmERFI-5</em>, respectively. We found that CmERFV-2 and CmERFI-5 could positively regulate the promoters of <em>CmVINV2</em> and <em>CmACO1</em>, respectively. The <em>agrobacterium</em>-mediated transient transformation further verified that CmERFV-2 silencing and CmERFI-5 overexpression resulted in higher sucrose levels and produced more ethylene. In short, postharvest low-temperature treatments promoted <em>CmERFV-2</em> expression and inhibited <em>CmERFI-5</em> expression, which activated the transcription of downstream <em>CmVINV2</em> and <em>CmACO1</em>, respectively, ultimately affecting sugar content and ethylene biosynthesis. The lower production of ethylene at low temperature reduced starch degradation, thereby affecting sugar accumulation in melon fruit. This study explained the cause for the lower sweetness of postharvest oriental melon fruit at low temperature and provided new insights into maintaining flavour quality at low temperature.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113295"},"PeriodicalIF":6.4,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships between melatonin and salicylic acid treatments in delaying the senescence of postharvest pear fruit","authors":"Ao Cheng ,&nbsp;Pengfei Zhao ,&nbsp;Xu Wang ,&nbsp;Shichao Luo ,&nbsp;Peixuan Xu ,&nbsp;Yiran Ye ,&nbsp;Hao Yuan ,&nbsp;Yuxin Ji ,&nbsp;Hui Ma ,&nbsp;Jianfeng Xu ,&nbsp;Yuxing Zhang ,&nbsp;Haixia Zhang","doi":"10.1016/j.postharvbio.2024.113288","DOIUrl":"10.1016/j.postharvbio.2024.113288","url":null,"abstract":"<div><div>Fruit senescence is an important stage in the post-ripening fruit life process and has a direct impact on the maintenance of postharvest fruit quality. Pears are among the most widely cultivated fruit trees in China and worldwide. However, there are problems such as poor postharvest preservation, high rotting rates, and serious economic losses in the pear industry. Previous studies have shown that melatonin and salicylic acid can delay fruit senescence; however, the relationship between them during this process has not been elucidated. Therefore, to clarify the relationships of melatonin and salicylic acid in regulating pear fruit senescence, this study applied melatonin, salicylic acid and their combined treatments at different concentrations to 'Xin li No.7′ pear fruit and analyzed the physiological, biochemical, and transcriptional effects of melatonin and salicylic acid on pear fruit senescence. The results showed that melatonin, salicylic acid and their combined treatments delayed the percentage of decayed pear fruit during postharvest storage. Melatonin, salicylic acid and their combined delayed the increase in weight loss of pear fruit and decrease in the internal quality (firmness, soluble solid content, and total soluble sugar) of pear fruit. This reduced the peak value of the endogenous ethylene release rate and the activity of cell wall-degrading enzymes (polygalacturonase, pectin methylesterase, and lipoxygenase). It also increased the activity of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase, and ascorbate peroxidase) and the levels of endogenous melatonin and salicylic acid in pears. However, melatonin and salicylic acid combined treatments did not present additive effects. In addition, the transcriptome data of pear peel and pulp treated with different concentrations of melatonin and salicylic acid at different concentrations during senescence storage were analysed using GO and KEGG enrichment to reveal the molecular relationships between them at the transcriptional level. In addition, we found that exogenous melatonin and salicylic acid upregulated the expression of <em>PR1-like</em> genes and downregulated the expression of <em>NPR-like</em> genes. They can also promote the expression of <em>ETR1</em> and <em>EBF-1</em> and inhibit the expression of <em>EIN3-like1</em> and <em>CTR1</em> in the ethylene signalling pathway in postharvest pear fruit. Our results preliminarily revealed the biochemical and molecular relationships between melatonin and salicylic acid in delaying pear fruit senescence and provided a theoretical basis to further elucidate the relationship between melatonin and salicylic acid in delaying the senescence of postharvest pear fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113288"},"PeriodicalIF":6.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-source feature stable learning method for rapid identification of cork spot disorder in ‘Akizuki’ pear","authors":"Jianghui Xiong ,&nbsp;Shangfeng Gu ,&nbsp;Yuan Rao ,&nbsp;Li Liu ,&nbsp;Xiaodan Zhang ,&nbsp;Yuting Wu ,&nbsp;Xiu Jin","doi":"10.1016/j.postharvbio.2024.113285","DOIUrl":"10.1016/j.postharvbio.2024.113285","url":null,"abstract":"<div><div>The quality grading and sorting of ‘Akizuki’ pear, a high-quality fruit, is a vital part of the supply chain. Meanwhile, cork spot disorder, a common physiological issue that affects the healthy development of pear fruit, requires rapid and accurate identification. To further enhance the identification precision of cork spot disorder in ‘Akizuki’ pear, this study proposes a multi-source feature stable learning (MFSL) method based on neural architecture search and sample reweighting techniques, building upon the fusion of near-infrared spectrum and visual image. This method employs secondary reweighted optimization training on a multi-source fusion model, enabling it to fully learn label-related features and thereby enhance generalization. Experimental results show that the optimal modelling performance of the multi-source fusion feature has increased by 26.89 % in accuracy compared to the single spectrum and by 11.19 % compared to the single image. After optimization training, the testing accuracy of the model improved by 1.31 %, reaching 89.47 %, and the F1-score increased by 1.47 %, reaching 89.83 %. The results validate the effectiveness of the method in enhancing the model’s generalization performance. The proposed MFSL method for the precise identification of cork spot disorder in ‘Akizuki’ pear in this study significantly improves the accuracy of the multi-source fusion model in symptom recognition. The research results have important reference value for the efficient grading and sorting of pear fruit quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113285"},"PeriodicalIF":6.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Starch–sucrose metabolism and phenylpropanoid biosynthesis pathways play crucial regulatory roles in extending the postharvest longevity of Lilium brownii var. viridulum bulbs","authors":"Zongquan Chen ,&nbsp;Haoran Zhu ,&nbsp;Kezhong Zhang ,&nbsp;Jinteng Cui","doi":"10.1016/j.postharvbio.2024.113280","DOIUrl":"10.1016/j.postharvbio.2024.113280","url":null,"abstract":"<div><div>The short shelf life of <em>Lilium brownii</em> var. <em>viridulum</em> bulbs after harvesting limits its market supply capacity and makes it difficult to meet customer demand. Therefore, it is particularly important to effectively extend the fresh-eating period of <em>Lilium brownii</em> var. <em>viridulum</em>. In this study, <em>Lilium brownii</em> var. <em>viridulum</em> was used as experimental material and metabolomics and transcriptomics techniques were employed. A total of 883 metabolites and 9452 differentially expressed genes were detected, primarily enriched in secondary metabolite biosynthesis, phenylpropanoid biosynthesis, and starch–sucrose metabolism pathways. Further analyses showed that the accumulation of cellulose had a positive effect on maintaining the cellular structure and morphology of the <em>Lilium brownii</em> var. <em>viridulum</em> bulbs. The reduction of vicianose may be related to the energy expenditure associated with maintaining internal metabolism in <em>Lilium brownii</em> var. <em>viridulum</em> bulbs after postharvest. The low temperature and hypobaric conditions could slow down the increase of reducing sugars, reduce the metabolic rate of sucrose, maintain the relative stability of trehalose, and improve the resistance of bulbs. Ferulic acid was not consumed in large quantities and flavonoids were not over-accumulated, effectively maintaining the stability of the epidermal colour of the bulbs. Meanwhile, the increased expression of <em>LbSUS4</em>, <em>LbINV2</em>, and <em>LbTPP</em> genes could enhance the cold tolerance of <em>Lilium brownii</em> var. <em>viridulum</em> bulbs, stabilise the cell membrane and protect the cell structure to adapt to changes in environmental conditions. In conclusion, bulbs stored under low temperature and hypobaric conditions showed minimal changes in metabolites, and their morphology and color were maintained for a longer duration. This method proves to be suitable for the rapid postharvest storage of <em>Lilium brownii</em> var. <em>viridulum</em> bulbs. This study provides new insights into the molecular regulatory mechanisms during postharvest storage of <em>Lilium brownii</em> var. <em>viridulum</em> bulbs and offers theoretical and technical support for extending their fresh-eating period.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113280"},"PeriodicalIF":6.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of apple defense protein MdPL and screening of proteins interaction with Penicillium expansum","authors":"Kaili Wang,&nbsp;Hua Wang,&nbsp;Meng Xu,&nbsp;Esa Abiso Godana,&nbsp;Yuchun Lu,&nbsp;Hongyin Zhang","doi":"10.1016/j.postharvbio.2024.113289","DOIUrl":"10.1016/j.postharvbio.2024.113289","url":null,"abstract":"<div><div>Blue mold disease, caused by <em>Penicillium expansum</em>, is the most devastating post-harvest fungal disease affecting apples. During the fungal infection, there is a significant interaction between the pathogen and the apple, leading to the production of important metabolites and the activation of related metabolic processes. As one of the metabolites, pectin lyase is an important pectin-modifying enzyme that regulates the elasticity and permeability of cell wall and plays a key role in plant disease resistance and fruit softening. In this study, a pectin lyase protein MdPL was identified, which has the function of regulating cell wall permeability. It is up-regulated in the process of apple defense against <em>P. expansum</em> infection, and may have a key defense function. Protein bioinformatics analysis showed that MdPL belonged to the PL1 family, and subcellular localization was predicted to be located in the nucleus. The protein expression of <em>P. expansum</em> at different stages of infection was analyzed by RT-qPCR. The results of subcellular localization of Agrobacterium transformation showed that MdPL was localized in the nucleus, which was consistent with the results of bioinformatics analysis. The Y2H system explored the proteins interacting with apple proteins during <em>P. expansum</em> infection. The results showed that apple bait protein had no self-activation and no toxic effect on Y2H cells. The interacting protein MdPL-PeXR was screened from 10 pairs of candidates interacting protein pairs. HDOCK was used to analyze the interaction protein of MdPL, the results showed that the docking score was −225.61 and the confidence score was 0.8194, which verified the results of the Y2H test. This study provides ideas and references for further exploring the interaction mechanism between apple and <em>P. expansum</em>, and is of great significance for the study of plant-pathogen interaction.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113289"},"PeriodicalIF":6.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MeJA and MeSA alleviate black rot in winter jujube caused by Alternaria tenuissima by regulating membrane lipid and reactive oxygen metabolism","authors":"Wanting Yang ,&nbsp;Lingling Li ,&nbsp;Yuxing Liu ,&nbsp;Weida Zhang ,&nbsp;Minrui Guo ,&nbsp;Guogang Chen","doi":"10.1016/j.postharvbio.2024.113275","DOIUrl":"10.1016/j.postharvbio.2024.113275","url":null,"abstract":"<div><div>Black rot is a serious disease caused by <em>Alternaria tenuissima</em> (<em>A. tenuissima</em>) that affects winter jujubes. This study explored the effects of MeJA and MeSA on disease resistance in winter jujubes by regulating membrane lipid and reactive oxygen metabolism in response to <em>A. tenuissima</em> infection. MeJA and MeSA significantly reduced the disease severity in winter jujube, enhanced the activity and gene expression of antioxidant-related enzymes, inhibited the activity and gene expression of membrane lipid oxidation-related enzymes, delayed the decomposition of ascorbic acid, glutathione, phosphatidylcholine, and phosphatidylinositol, inhibited the accumulation of hydrogen peroxide, phosphatidic acid, and malondialdehyde, the production rate of superoxide anion and a rise in relative electrical conductivity, and maintained winter jujube cell membrane unsaturation (U/S). Compared with the sterile water treatment, the regulation of membrane lipid metabolism and ROS metabolism in winter jujube fruits by MeJA and MeSA could be a contributing factor to their enhanced resistance against <em>A. tenuissima</em>. The membrane lipid oxidation degree in the MeJA group was lower, indicating that MeJA had better application prospects in disease resistance and storing and preserving winter jujube than MeSA.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113275"},"PeriodicalIF":6.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide characterization and comparative transcriptomics unravel CpMADS47 as a positive regulator during fruit ripening and softening in Chinese cherry","authors":"Tai Tian ,&nbsp;Shiqing Yin ,&nbsp;Fengting Huang ,&nbsp;Longqiang Feng ,&nbsp;Yan Ma ,&nbsp;Hao Wang ,&nbsp;Jing Zhang ,&nbsp;Wen He ,&nbsp;Yuanxiu Lin ,&nbsp;Yunting Zhang ,&nbsp;Mengyao Li ,&nbsp;Zhiwei Wu ,&nbsp;Yong Zhang ,&nbsp;Ya Luo ,&nbsp;Haoru Tang ,&nbsp;Qing Chen ,&nbsp;Xiaorong Wang ,&nbsp;Yan Wang","doi":"10.1016/j.postharvbio.2024.113287","DOIUrl":"10.1016/j.postharvbio.2024.113287","url":null,"abstract":"<div><div>Chinese cherry [<em>Cerasus pseudocerasus</em> (Lindl.) G.Don] (syn. <em>Prunus pseudocerasus</em> Lindl.) is an economically important fruit crop native to China. The fruits are prone to softening and rotting after harvest, which significantly limits their marketability and hinders its rapid development throughout China. The MADS-box gene family, particularly the SEP subfamily, plays a crucial role in governing fruit ripening and softening. However, the molecular mechanisms underlying fruit ripening and softening in Chinese cherry remains unclear. Herein, we identified 92 MADS genes from the Chinese cherry genome and analyzed their physicochemical characteristics, chromosomal localization, phylogeny, gene structures, covariance, and cis-acting elements. Many <em>cis</em>-elements in the promoters of <em>CpMADSs</em> are implicated in fruit development, ripening and stress response. Using comparative transcriptomics and RT-qPCR analysis, we identified a key gene, <em>CpMADS47</em>, as a positive regulator of cherry fruit ripening. CpMADS47 is localized in both the nucleus and cell membrane and shows highly expression in flowers and mature fruits. Transient overexpression of <em>CpMADS47</em> in cherry fruit demonstrated its role in mediating fruit ripening and softening by promoting reduction in fruit firmness, anthocyanin accumulation, depolymerization of cell wall components, enhancement of cell wall degradation enzyme activity, and ABA biosynthesis. Conversely, silencing <em>CpMADS47</em> generated the opposite effect. Yeast one-hybrid and dual-luciferase assays revealed that the <em>CpMADS47</em> targets the promoters of cell wall degrading genes (<em>CpPME3</em> and <em>CpXTH31</em>) and ABA signal transduction genes (<em>CpPP2C12</em>), thereby activating their transcription and promoting cherry fruit ripening. In summary, this study enriches our understanding of the transcriptional regulation of fruit ripening and softening in Chinese cherry.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113287"},"PeriodicalIF":6.4,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}