Postharvest Biology and Technology最新文献

{"title":"Developing composite biocontrol agents based on microbiome dynamics during postharvest storage of tomatoes","authors":"Lu Zhuang ,&nbsp;Xiaoyun Zhang ,&nbsp;Solairaj Dhanasekaran ,&nbsp;Esa Abiso Godana ,&nbsp;Jun Li ,&nbsp;Ruijie Luo ,&nbsp;Lina Zhao ,&nbsp;Yu Li ,&nbsp;Xiaoyong Liu ,&nbsp;Hongyin Zhang","doi":"10.1016/j.postharvbio.2025.113509","DOIUrl":"10.1016/j.postharvbio.2025.113509","url":null,"abstract":"<div><div>Single antagonistic microorganisms introduced into the microbial community on the fruit surface often show poor adaptation, resulting in less efficient and inconsistent pathogen inhibition, whereas the interaction of composite microorganisms can increase their ability to combat plant diseases. This study used high-throughput sequencing techniques to explore the dynamics of bacterial and fungal communities on the surface of postharvest tomatoes during storage. Subsequently, potentially beneficial core microorganisms were identified based on correction correlations with one another, and microbial formulations were developed using the isolated beneficial core microorganisms with control efficacy. Based on the microbiome analysis results, two pathogens were screened and identifi<em>ed</em> as <em>Alternaria tenuissima</em> and <em>Cladosporium cladosporioides.</em> Among the five potentially beneficial core microorganisms that were isolated, <em>Bacillus velezensis</em> B1, <em>Pantoea agglomerans</em> B10, and <em>Meyerozyma caribbica</em> Y9, demonstrated safety and highest control efficacy. Considering the affinity between strains, <em>M. caribbica</em> Y9 was combined with either <em>P. agglomerans</em> B10 or <em>B. velezensis</em> B1 to formulate composite microorganisms. The <em>in vivo</em> results demonstrated that all composite microorganisms were more effective in controlling the postharvest diseases caused by <em>A. tenuissima</em> and <em>C. cladosporioides</em> than the single microorganism and <em>P. agglomerans</em> B10 and <em>M. caribbica</em> Y9 at the ratio of 2:1 being the most effective. Therefore, the composite microorganisms formulated with the isolated strains from fresh tomatoes could significantly enhance the control effect, demonstrating strong potential for inhibiting postharvest diseases in tomatoes and promising prospects for widespread application.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113509"},"PeriodicalIF":6.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo transcriptome assembly and analysis unveil molecular insights into Cladosporidium rot development in harvested table grapes","authors":"Solairaj Dhanasekaran,&nbsp;Michael Ackah,&nbsp;Qiya Yang,&nbsp;Hongyin Zhang","doi":"10.1016/j.postharvbio.2025.113497","DOIUrl":"10.1016/j.postharvbio.2025.113497","url":null,"abstract":"<div><div>Cladosporium cladosporioides is a fungal pathogen that affects various crops worldwide, including table grapes, where it causes Cladosporium rot. The molecular episodes of Cladosporium rot development are still unclear and need to be explored. More detailed knowledge of the molecular mechanisms underlying Cladosporium rot will provide upcoming developments on disease resistance and management. Here, we report RNA sequencing and transcriptomic analysis of C. cladosporioides infecting postharvest table grapes and its relationship with the grape’s defense response. High-throughput sequencing of cDNA libraries following de novo transcriptome assembly produced 70 thousand transcripts. This study is the first attempt to explore the transcriptional level changes of the fungus during Cladosporium rot development in table grapes. Our study showed that the fungus expresses huge sets of pathogenicity-related genes and families of genes encoding CAZymes, transcription factors and secretory proteins. Further, we identified genes of this fungus that facilitate nutrient uptake, metabolism and growth in grapes, which were significantly upregulated. This study provides valuable insights into the complex pathogenic mechanisms of C. cladosporioides, in which the fungi alter its transcriptome and develop adaptations to overcome the grape’s defenses and colonize table grape tissue.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113497"},"PeriodicalIF":6.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Aspergillus flavus contamination in peanut kernels using a hybrid convolutional transformer-feature fusion network: A macro-micro integrated hyperspectral imaging approach and two-dimensional correlation spectroscopy analysis","authors":"Zhen Guo ,&nbsp;Haifang Wang ,&nbsp;Fernando A. Auat-Cheein ,&nbsp;Zhishang Ren ,&nbsp;Lianming Xia ,&nbsp;Ibrahim A. Darwish ,&nbsp;Yemin Guo ,&nbsp;Xia Sun","doi":"10.1016/j.postharvbio.2025.113489","DOIUrl":"10.1016/j.postharvbio.2025.113489","url":null,"abstract":"<div><div><em>Aspergillus flavus</em> contamination in peanut kernels poses significant health risks and economic losses, hence requiring accurate and fast detection methods to ensure postharvest safety and quality. This study investigated the detection of <em>Aspergillus flavus</em> contamination in peanut kernels using visible-near infrared (VNIR) hyperspectral imaging and hyperspectral microscopic imaging (HMI). The research explored the structural damage to peanut kernel cells and tissue caused by contamination, as revealed through both electron microscopy and hyperspectral imaging. Generalized two-dimensional correlation spectroscopy analysis was applied to determine the sequence of molecular changes, providing insights into fungal metabolism. The spatial-spectral features of the peanut kernels and peanut kernel sections were extracted, and a hybrid convolutional transformer-feature fusion network (HCT-FFN) was employed for features integration and classification. The model demonstrated superior accuracy compared to classic deep learning models, with test accuracy of 100.00 % for both VNIR hyperspectral imaging and HMI. Using smaller regions of interest in peanut kernel sections maintained high accuracy and improved the efficiency of the model. The study concluded that <em>Aspergillus flavus</em> contamination significantly altered peanut kernel structure and spectral properties. The HCT-FFN model proved highly effective for detecting and classifying contamination with minimal computational costs, highlighting its potential as a valuable tool for ensuring the safety and quality of postharvest nuts.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113489"},"PeriodicalIF":6.4,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol effects of volatile organic compounds released from Burkholderia sola NAU20 to strawberry anthracnose caused by Colletotrichum gloeosporioides","authors":"Dongming Wang,&nbsp;Zhitao Geng,&nbsp;Jiming Shen,&nbsp;Tingting Xie,&nbsp;Fan Wu,&nbsp;Yu Zhang,&nbsp;Yiwei Dong,&nbsp;Dongmei Mao,&nbsp;Yanling Ji,&nbsp;Yan Huang,&nbsp;Zhoukun Li,&nbsp;Yongheng Liang,&nbsp;Xianfeng Ye,&nbsp;Zhongli Cui","doi":"10.1016/j.postharvbio.2025.113510","DOIUrl":"10.1016/j.postharvbio.2025.113510","url":null,"abstract":"<div><div>Strawberry anthracnose is a fungal disease caused by <em>Colletotrichum gloeosporioides</em>, which can lead to significant economic losses in postharvest strawberries. Biological control is a greener and safer strategy. In this study, the hyphal growth and conidial germination of <em>Colletotrichum gloeosporioides</em> were found to be significantly inhibited by volatile compounds (VOCs) produced by <em>Burkholderia sola</em> NAU20. Ethyl trans-2-octenoate demonstrated the strongest antifungal activity among the tested VOCs, with a minimum inhibitory concentration (MIC) of 0.448 mL L⁻¹. The structure of fungal cell wall was severely disrupted, and a significant accumulation of ROS and DNA fragmentation indicated that apoptosis may be induced in ethyl trans-2-octenoate treated cells. Ethyl trans-2-octenoate can completely inhibit the infection of <em>C. gloeosporioides</em> on strawberries at a level of 2 ×MIC within 5 d. Furthermore, in addition to the direct antifungal effect of ethyl trans-2-octenoate, the induction of phenylpropanoids and flavonoids biosynthesis enhanced the suppression of fungal infection.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113510"},"PeriodicalIF":6.4,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transfer of apple soluble solids content prediction model across cultivars based on domain-adversarial neural network","authors":"Jiabao Li ,&nbsp;Jianing Luo ,&nbsp;Qingji Tian ,&nbsp;Shanghong Yang ,&nbsp;Youhua Bu ,&nbsp;Qian Chi ,&nbsp;Wenchuan Guo","doi":"10.1016/j.postharvbio.2025.113494","DOIUrl":"10.1016/j.postharvbio.2025.113494","url":null,"abstract":"<div><div>Poor model generalizability has become a key problem in using visible and near-infrared (Vis-NIR) spectroscopy to detect the internal quality of fruit, which is due to the differences in the physicochemical properties and spectral distributions of fruit under different cultivars, leading to the fact that the model established for a certain cultivar cannot effectively predict the internal quality under other cultivars. To solve this problem, 'Ruixue', Luochuan 'Fuji', and Jingning 'Fuji' were used as samples to compare the transfer performances of three transfer methods, namely, two-stage TrAdaBoost.R2 (TTB), fine-tune (FT), and domain-adversarial neural network (DANN). The results showed that the model based on DANN could effectively eliminate the variability among different cultivars in the same region and year, which was more effective in transferring the prediction model of soluble solids content (SSC). In the transfer of 'Ruixue' to Luochuan 'Fuji', when samples of the target domain were 67, the <span><math><msub><mrow><mi>R</mi></mrow><mrow><mi>p</mi></mrow></msub></math></span> was 0.93, and the <span><math><mtext>RMSEP</mtext></math></span> was 0.56 %, confirming the potential of DANN in solving poor model generalization.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113494"},"PeriodicalIF":6.4,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and feasibility of an approach utilizing modified atmosphere packaging (MAP) and ethylene scrubbing to allow extended international transport and marketing of tree-ripe mangos","authors":"Faisal Shahzad,&nbsp;Moshe Doron,&nbsp;Steven A. Sargent,&nbsp;Jeffrey K. Brecht","doi":"10.1016/j.postharvbio.2025.113488","DOIUrl":"10.1016/j.postharvbio.2025.113488","url":null,"abstract":"<div><div>The feasibility of incorporating modified atmosphere packaging (MAP) technology with or without ethylene scrubbing into existing mango international transport and marketing systems was evaluated as a way to allow the shipment of tree-ripe fruit to improve the quality of mangos available to consumers in the destination countries. In preliminary research, the optimum atmospheres (low O₂; high CO₂) for slowing ‘Tommy Atkins’ and ‘Keitt’ mango ripening beginning at the tree-ripe (half ripe) developmental stage were determined using controlled atmosphere (CA). BreatheWay® MAP and RYPEN^{<span>tm</span>} ethylene control systems were then custom designed for us by the companies based on the results of the CA experiments. The preliminary research established that tree-ripe mangos (Stage 3.0–3.5 on a 1–5 scale) can be stored as low as 7 °C for 4 weeks without showing chilling injury (CI) symptoms or deterioration of fruit internal quality when held at a minimum 4 kPa O₂ (‘Keitt’) or 6 kPa O₂ (‘Tommy Atkins’) plus 5–10 kPa CO₂. The MAP attained atmospheres similar to the optimum CA (average values of 6 kPa O₂ + 9 kPa CO₂ for both cultivars) for 4 weeks of storage at 7 °C. Ethylene scrubbing reduced ethylene concentration inside the MAP from 1.03 μL/L to 0.16 μL/L during storage at 7 °C. The MAP and ethylene scrubbing resulted in desired quality attributes (reduced CI, lenticel discoloration, and decay, plus higher fruit firmness and titratable acidity, and better organoleptic traits including acidity and overall eating quality) of tree-ripe mangos of both cultivars during ambient temperature (20 °C) shelf life. To summarize, MAP and ethylene scrubbing, alone or in combination, slowed down ripening related softening, color changes, and losses of sugars and acids, while sensory panelists rated the treated fruit as exhibiting higher overall eating quality than the control, which were overripe. Our results lead us to recommend BreatheWay® MAP and RYPEN^{<span>tm</span>} technology or their equivalent for long distance (4-week) shipping if the goal is to export/import tree-ripe mangos.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113488"},"PeriodicalIF":6.4,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergic use of essential oil and sous-vide packaging for ready-to-cook organic vs. conventional globe artichoke heads","authors":"Valeria Rizzo ,&nbsp;Sara Lombardo ,&nbsp;Viviana Brischetto ,&nbsp;Gaetano Pandino ,&nbsp;Claudia Formenti ,&nbsp;Giovanni Mauromicale ,&nbsp;Riccardo Nunzio Barbagallo ,&nbsp;Lucia Parafati ,&nbsp;Cristina Restuccia ,&nbsp;Giuseppe Muratore","doi":"10.1016/j.postharvbio.2025.113492","DOIUrl":"10.1016/j.postharvbio.2025.113492","url":null,"abstract":"<div><div>Given the reduced production in organic farming and its higher costs, the present study aimed to define the suitability of organically grown (ORG) globe artichoke heads of cv. Tema 2000 to be processed as fresh-cut product in comparison with conventionally grown (CONV) ones. The study investigated the combined use of parsley essential oil (EO) and biodegradable cellulose pouches suitable for hypobaric packaging (CELL) as a sustainable alternative to conventional plastic bags (OPA) during 22 days of refrigerated storage. Results indicated that EO addition was extremely effective in improving the physical properties of CELL pouches and limiting the microbial growth during artichoke storage, even in ORG samples, which are typically more contaminated due to natural fertiliser use. Considering the importance of a biodegradable packaging choice, the EO treatment improved the CELL performance and prolonged the shelf life, suggesting that this treatment is promising for fresh-cut globe artichoke processing.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"225 ","pages":"Article 113492"},"PeriodicalIF":6.4,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of kiwifruit with Lactiplantibacillus pentosus CW5 cell-free supernatant maintains postharvest quality and alters the surface microbiome","authors":"Yuxuan Hao ,&nbsp;Huili Pang ,&nbsp;Haoxin Lv ,&nbsp;Guofang Wu ,&nbsp;Jianbo Zhang ,&nbsp;Xuan Luo ,&nbsp;Miao Zhang ,&nbsp;Yaoke Duan ,&nbsp;Yuting Hou ,&nbsp;Yanping Wang ,&nbsp;Lei Wang ,&nbsp;Yimin Cai ,&nbsp;Zhongfang Tan","doi":"10.1016/j.postharvbio.2025.113478","DOIUrl":"10.1016/j.postharvbio.2025.113478","url":null,"abstract":"<div><div>Kiwifruit is a nutritious, flavorful fruit that is popular with consumers. However, kiwifruit is a climacteric fruit that is prone to rapid softening and postharvest diseases during storage. In this regard, the application of lactic acid bacteria has been shown to preserve fruit quality and inhibit disease development. In this study, the effect of the application of <em>Lactiplantibacillus pentosus</em> CW5 cell-free supernatant (<em>L. pentosus</em> CW5 CFS) on the postharvest preservation of kiwifruit quality was comprehensively investigated using visual observations, the assessment of several quality indices and antioxidant capacity, and an investigation of the microbial community present on the kiwifruit surface. Results indicated that, relative to the control group (CK), <em>L. pentosus</em> CW5 CFS attenuated the rate of softening and rot development in stored kiwifruit, delayed the increase in soluble solids content (SSC), decreased the reduction in ascorbic acid observed at the end of storage, and maintained SOD, POD, and APX antioxidant enzyme activity. <em>L. pentosus</em> CW5 CFS also reduced the relative abundance of several postharvest pathogenic fungi present in the surface microbiota of kiwifruit, suggesting that this may be a factor in its preservation effect. Our study demonstrated the potential use of <em>L. pentosus</em> CW5 CFS in the postharvest preservation of kiwifruit and provided information that could be used in the development of safe and effective postharvest preservation technologies.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"224 ","pages":"Article 113478"},"PeriodicalIF":6.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding citrus aroma: Changes induced by postharvest treatments of fresh fruit","authors":"Shuxun Liu,&nbsp;Yuling Cai,&nbsp;Ying Lou,&nbsp;Yan Zhao,&nbsp;Mingfeng Cao,&nbsp;Ping Li,&nbsp;Qing Gu","doi":"10.1016/j.postharvbio.2025.113491","DOIUrl":"10.1016/j.postharvbio.2025.113491","url":null,"abstract":"<div><div>Citrus fruit are among the most economically important crops globally, with substantial production and trade volumes. Due to their high yield and concentrated harvest period, a portion of the fruit undergo postharvest treatments to improve their shelf life. However, during the treatments, the reduction in the content of unique aroma compounds and the formation of off-flavors may decrease their commercial value. Given that aroma quality is a decisive factor in determining the marketability of citrus fruit, implementing effective postharvest treatments is essential for maintaining fruit quality and enhancing their commercial value. This study systematically reviews the effects of different postharvest treatments aimed at extending the shelf life of citrus, including cold storage, coating and packaging, and pre-storage treatments (heat treatment, irradiation treatment, electrical treatment, ultrasound treatment, and ethylene/ethephon treatment) on the aroma quality of citrus fruit. By exploring the alterations in the aroma profile induced by these technologies, this review provides a theoretical framework for optimizing postharvest aroma preservation, supporting both quality maintenance and product value enhancement in the citrus industry.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"224 ","pages":"Article 113491"},"PeriodicalIF":6.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ozone treatment controlled postharvest table grape blue mold by targeting cell membrane to cause membrane integrity disruption and inhibited the development of Talaromyces rugulosus and accumulation of ochratoxin A","authors":"Jinzhu Li ,&nbsp;Jihui Xi ,&nbsp;Ruoxing Wang ,&nbsp;Kunhao Jiang ,&nbsp;Xiao Li ,&nbsp;Qingru Zhang ,&nbsp;Huali Xue ,&nbsp;Yang Bi","doi":"10.1016/j.postharvbio.2025.113490","DOIUrl":"10.1016/j.postharvbio.2025.113490","url":null,"abstract":"<div><div><em>Talaromyces rugulosus</em> is a prevalent and devastating fungus responsible for table grapes blue mold and is capable of metabolizing ochratoxin A (OTA), which leads to substantial economic losses and poses potential health risks to human. This study investigates the effects of ozone fumigation on controlling postharvest blue mold caused by <em>T. rugulosus</em>, as well as the potential mechanisms of action. The results demonstrated that ozone treatment (2.0 mg L⁻¹) inhibited the expansion of blue mold caused by <em>T. rugulosus</em> in both ‘Sunshine Rose’ and ‘Red Globe’ grape varieties. <em>In vitro</em> experiments showed that ozone suppressed mycelial growth and spore germination by altering the ultrastructural morphology of both spores and hyphae. In addition, ozone treatment led to a significant improvement in reactive oxygen species (ROS) levels that increased malondialdehyde (MDA) content, electrical conductivity and caused leakage of proteins and nucleic acids, ultimately, deeply damaging cell membrane integrity. Molecular docking experiments indicated that ozone interacted with key proteins, ERG6 and ERG27, involved in ergosterol synthesis on the cell membrane, forming intermolecular hydrogen bonds. This interaction downregulated the relative expression of <em>TrERG6</em> and <em>TrERG27</em>, thereby inhibiting ergosterol synthesis and reducing ergosterol content. Furthermore, ozone also reduced OTA accumulation by down-regulating the relative expression of crucial genes involved in OTA biosynthesis, including polyketide synthase (<em>PKS</em>), non-ribosomal peptide synthetase (<em>NRPS</em>), cytochrome P450 (<em>P450</em>), and ethanol dehydrogenase (<em>ADH</em>). Finally, ozone decreased weight loss, delayed the decline in the total soluble solids, vitamin C content, titratable acid and reducing sugars, which maintained the nutritional and edible quality of the grapes.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"224 ","pages":"Article 113490"},"PeriodicalIF":6.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}