Ozone treatment controlled postharvest table grape blue mold by targeting cell membrane to cause membrane integrity disruption and inhibited the development of Talaromyces rugulosus and accumulation of ochratoxin A

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-03-01 DOI:10.1016/j.postharvbio.2025.113490

Jinzhu Li , Jihui Xi , Ruoxing Wang , Kunhao Jiang , Xiao Li , Qingru Zhang , Huali Xue , Yang Bi

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引用次数: 0

Abstract

Talaromyces rugulosus is a prevalent and devastating fungus responsible for table grapes blue mold and is capable of metabolizing ochratoxin A (OTA), which leads to substantial economic losses and poses potential health risks to human. This study investigates the effects of ozone fumigation on controlling postharvest blue mold caused by T. rugulosus, as well as the potential mechanisms of action. The results demonstrated that ozone treatment (2.0 mg L⁻¹) inhibited the expansion of blue mold caused by T. rugulosus in both ‘Sunshine Rose’ and ‘Red Globe’ grape varieties. In vitro experiments showed that ozone suppressed mycelial growth and spore germination by altering the ultrastructural morphology of both spores and hyphae. In addition, ozone treatment led to a significant improvement in reactive oxygen species (ROS) levels that increased malondialdehyde (MDA) content, electrical conductivity and caused leakage of proteins and nucleic acids, ultimately, deeply damaging cell membrane integrity. Molecular docking experiments indicated that ozone interacted with key proteins, ERG6 and ERG27, involved in ergosterol synthesis on the cell membrane, forming intermolecular hydrogen bonds. This interaction downregulated the relative expression of TrERG6 and TrERG27, thereby inhibiting ergosterol synthesis and reducing ergosterol content. Furthermore, ozone also reduced OTA accumulation by down-regulating the relative expression of crucial genes involved in OTA biosynthesis, including polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS), cytochrome P450 (P450), and ethanol dehydrogenase (ADH). Finally, ozone decreased weight loss, delayed the decline in the total soluble solids, vitamin C content, titratable acid and reducing sugars, which maintained the nutritional and edible quality of the grapes.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.