Biao Wang , Jianmin Yun , Weihong Guo , Jiawei Shen , Ting Wang , Fengyun Zhao , Yuling Qu , Liang Yao
{"title":"Transcriptomic analysis reveals the mechanism of ozone fumigation combined with polyethylene nanopackaging for delaying the browning and softening of mushrooms (Agaricus bisporus)","authors":"Biao Wang , Jianmin Yun , Weihong Guo , Jiawei Shen , Ting Wang , Fengyun Zhao , Yuling Qu , Liang Yao","doi":"10.1016/j.postharvbio.2024.113272","DOIUrl":"10.1016/j.postharvbio.2024.113272","url":null,"abstract":"<div><div>Browning and softening are typical characteristics of the postharvest quality degradation of button mushrooms. In this paper, comparative transcriptome analyses of mushroom samples during storage have been established based on sensory evaluations and physiological and biochemical indices analyses used to investigate the delaying effects of ozone fumigation combined with polyethylene nanopackaging (OCNP) on the browning and softening process, as well as their potential regulatory mechanisms. Our results indicated that the OCNP treatment reduced the respiratory rate of <em>Agaricus bisporus</em>, decreased the browning index and electrolyte leakage rate, and maintained the whiteness and firmness of postharvest mushrooms. In addition, OCNP inhibited the activities of browning-related enzymes (polyphenol oxidase and tyrosinase) and cell wall degrading enzymes (chitinase and β-1,3-glucanase) in the fruiting bodies, which delayed softening, while attenuating browning in <em>A. bisporus</em>. Transcriptomics analyses have revealed that OCNP inhibited the occurrence of browning by down-regulating genes involved in the antioxidant system (catalase and superoxide dismutase) as well as those related to browning enzymes (polyphenol oxidase and tyrosinase). Furthermore, OCNP also maintained the integrity of the cell walls by down-regulating genes associated with the degradation of cell wall components, such as chitin and β-1,3-glucan, thereby postponing softening. In summary, OCNP delayed the postharvest browning and softening of mushrooms by inhibiting the activity of browning-associated enzymes and mitigating the loss of cell wall components.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113272"},"PeriodicalIF":6.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liang Shuai , PengYu Xue , Lingyan Liao , Xiaojuan Guo , Yunfen Liu , Mubo Song , Wen Cai , Feilong Yin , Meiying He
{"title":"Methyl jasmonate improves pulp flavor by modulating sugar metabolism in postharvest longan fruit","authors":"Liang Shuai , PengYu Xue , Lingyan Liao , Xiaojuan Guo , Yunfen Liu , Mubo Song , Wen Cai , Feilong Yin , Meiying He","doi":"10.1016/j.postharvbio.2024.113268","DOIUrl":"10.1016/j.postharvbio.2024.113268","url":null,"abstract":"<div><div>Flavor deterioration reduces the postharvest quality of longan fruit. As an important plant endogenous environmental responsive hormone, the effects of methyl jasmonate (MeJA) treatment on the sugar metabolism of postharvest longan fruit remains to be elucidated. In present study, fruit treated with 100 μmol L<sup>−1</sup> MeJA exhibited higher levels of total soluble solids (TSS), sucrose, and total sugar, while lower levels of glucose and fructose. These results are attributed to the inhibited of activities of acid invertase (AI), neutral invertase (NI), and sucrose synthase-cleavage direction (SS-c) in MeJA-treated longan fruit which promote the decomposition of sucrose into glucose and fructose. Additionally, there was an increase in sucrose synthase-synthesis direction (SS-s) and sucrose phosphate synthase (SPS) activities, facilitating sucrose accumulation. Elevated fructokinase (FRK) and hexokinase (HXK) activities in MeJA-treated longan fruit led to a better utilization of glucose and fructose in the glycolysis pathway, thus providing energy for postharvest longan fruit. Compared to the control fruit, the MeJA treatment up-regulated the transcript levels of <em>SS</em> (<em>DlSS-1, DlSS-2</em>, and <em>DlSS-3</em>), <em>DlSPS</em>, <em>DlFRK</em>, and <em>DlHXK</em>, while down-regulating the transcript levels of <em>AI</em> (<em>Cell wall acid invertase</em> (<em>DlCWAI</em>) and <em>soluble acid invertase</em> (<em>DlSAI</em>)), and <em>NI</em> (<em>DlNI-2</em> and <em>DlNI-3</em>). In the <em>SS</em> gene, correlation analysis showed that <em>DlSS-1</em> mainly contributes to promote the sucrose synthesis. These findings suggest that MeJA treatment could improve the status of sugar by modulating the enzyme activities and the expression of genes associated with sugar metabolism, thereby preserving the flavor quality of longan fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113268"},"PeriodicalIF":6.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liv Gilpin , Fabrizio Costa , Nicholas P. Howard , Dag Røen , Muath Alsheikh
{"title":"Multiple genetic analyses disclose the QTL dynamic for fruit texture and storability in Norwegian apples (Malus domestica Borkh.)","authors":"Liv Gilpin , Fabrizio Costa , Nicholas P. Howard , Dag Røen , Muath Alsheikh","doi":"10.1016/j.postharvbio.2024.113276","DOIUrl":"10.1016/j.postharvbio.2024.113276","url":null,"abstract":"<div><div>In Norway, apples (<em>Malus domestica</em> Borkh.) are produced at latitude around 60° north. Notably the season is short and cool and Norwegian cultivars have developed under selection pressure from these distinct climatic conditions, resulting in apple germplasm with unique genetic structure and pedigree. Strong selection for earliness has resulted in several cultivars that mature and soften quickly, making it challenging to meet consumer expectations for apple quality. The commercial success of apple is largely related to its texture and long-term storability, enabling a year-round availability of fresh fruit. Texture in apple has been well characterized and major causative genes have been found. Nonetheless, comprehensive knowledge of the genetic control of texture retention is lacking. To improve postharvest performance, including storability, in the breeding program currently ongoing at Njøs Fruit and Berry Centre (NJØS), a diversity collection of 197 apple cultivars was employed to initiate a genome-wide association analysis (GWAS) to identify relevant genomic regions associated with these aspects. Quantitative trait loci (QTL) associated with different dissected multi-trait texture components assessed by a texture analyzer equipped with an acoustic device were identified. To target QTLs relevant to improving postharvest storage, a softening and storage index was also implemented into the QTL analysis, further mapped on chromosome 10. The GWAS-QTL pattern was additionally validated on a different genetic background, implementing a multi-parental-cross-design scheme. Findings include previously unreported genomic regions related to texture attributes, and especially haploblock HB-10–03 represents an important novel molecular tool valuable for breeding Norwegian apple cultivars with superior fruit storability.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113276"},"PeriodicalIF":6.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xianzhu Deng, Yingying Wei, Shu Jiang, Jianfen Ye, Yi Chen, Feng Xu, Jiahui Chen, Xingfeng Shao
{"title":"Recent advances in the application of tea tree oil in the storage of fruit and vegetables","authors":"Xianzhu Deng, Yingying Wei, Shu Jiang, Jianfen Ye, Yi Chen, Feng Xu, Jiahui Chen, Xingfeng Shao","doi":"10.1016/j.postharvbio.2024.113260","DOIUrl":"10.1016/j.postharvbio.2024.113260","url":null,"abstract":"<div><div>Fruit and vegetables production is permanently affected by many threats including fungal pathogens, which leads to serious losses from harvest to final consumption. Chemical preservatives are effective but may pose an increased risk of drug resistance and contamination to the environment. The demand for natural substances as fungicides is gradually increasing. Tea tree oil (TTO) obtained by steam distillation of <em>Melaleuca alternifolia</em>, has been widely used as ‘green’ and ‘safety’ antifungal agent for many years. This paper comprehensively summarized the source, composition, extraction methods and biological functions of TTO and its applications in the storage of fruit and vegetables. In particular, we focused on the functions of TTO as antimicrobial agents to reduce the occurrence and spread of diseases and activate plants' defense system, including preservation effects, antimicrobial mechanisms and application methods. This was highly intended to help and serve as a reference for the pertinent researchers.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113260"},"PeriodicalIF":6.4,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ascorbic acid mitigates ROS-induced chilling injury development in postharvest sweet basil via improving antioxidant defense system to impede senescence process","authors":"Sirawich Chotikakham , Natthapong Janhom","doi":"10.1016/j.postharvbio.2024.113265","DOIUrl":"10.1016/j.postharvbio.2024.113265","url":null,"abstract":"<div><div>The visible manifestations of chilling injury symptoms significantly worsen nutritional and quality losses in sweet basil, rendering low-temperature storage impractical for extending its postharvest longevity. This study sought to determine how immersion in ascorbic acid (AsA) affected the visible development of chilling injury symptoms (leaf discoloration and death lesion manipulation) in sweet basil leaves, specifically focusing on oxidative damage, chlorophyll degradation and cell death. After being submerged in AsA at concentrations of 0, 1, 5 and 10 mM for 20 min, sweet basil branches were kept at 6 ± 1 °C for 12 d. The best concentration (10 mM) in alleviating chilling injury development and maintaining leaf quality was underwent analysis for reactive oxygen species (ROS) accumulations, antioxidant systems, chlorophyll degradation and cell death. The visible sight of chilling injury initially appeared on day 3 coinciding with gradually elevated levels of ROS (superoxide and hydrogen peroxide), malondialdehyde and caspase 3, 8 and 9-like activities. Moreover, a surging in leaf discoloration coincided with the reduction in the rate of electron transport in isolated chloroplast and chlorophyll contents. However, the AsA-treated group exhibited lower chilling injury (25–60 %) than control on day 3 to day 12, which was associated with enhanced antioxidant systems, decreased ROS accumulation and lower activities of chlorophyll degrading enzymes (chlorophyll peroxidase and chlorophyllase) as well as caspase-like activities. These findings suggested that AsA immersion elevated antioxidant activity to suppress oxidative damage, reduced chlorophyll degradation and mitigated cell death, thereby improving cold stress tolerance in sweet basil during storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113265"},"PeriodicalIF":6.4,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phytosulfokine treatment delays browning of litchi pericarps during storage at room temperature","authors":"Hanzhi Liang , Yanxuan Zhu , Zhiwei Li , Yueming Jiang , Xuewu Duan , Guoxiang Jiang","doi":"10.1016/j.postharvbio.2024.113262","DOIUrl":"10.1016/j.postharvbio.2024.113262","url":null,"abstract":"<div><div>Litchi, a tropical and subtropical fruit with substantial commercial value, is prone to browning after harvest. Phytosulfokine (PSK), a plant peptide hormone, plays a significant role in postharvest fruit preservation by affecting various biological processes. However, its specific effects on litchi browning have not been fully elucidated. This study demonstrates that PSK application effectively delays litchi pericarp browning, maintains a higher TSS/TA ratio, and reduces electrical conductivity. PSK treatment inhibits the degradation of anthocyanins, flavonoids, and phenolics, while also decreasing the accumulation of hydrogen peroxide (H₂O₂), malondialdehyde (MDA), and superoxide anions (O₂⁻). Additionally, PSK enhances the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR), while reducing the activities of ascorbate peroxidase (APX), polyphenol oxidase (PPO), and peroxidase (POD) compared to the control. Transcriptome analysis reveals that PSK upregulates genes involved in plant hormone signaling and biosynthesis of anthocyanins and flavonoids, while downregulating genes associated with glutathione metabolism and autophagy. RT-qPCR confirms that PSK treatment decreases the expression levels of <em>APX</em>, <em>LAC7</em>, <em>PPO</em>, <em>POD1</em>, <em>POD5</em>, <em>POD51</em>, and <em>PODX</em>, while increasing the expression of <em>GPX4</em>, contributing to the delayed browning. These findings suggest that PSK mitigates litchi browning by modulating oxidative enzymatic reactions and suppressing the expression of browning-related genes.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113262"},"PeriodicalIF":6.4,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huili Zhang , Tingting Shan , Yang Chen , Hongyi Wen , Mengshi Lin , Zhongqi Fan , Hui Wang , Yihui Chen , Hetong Lin , Yifen Lin
{"title":"Salicylic acid treatment improves the storability of fresh longan by regulating the metabolisms of respiration and energy","authors":"Huili Zhang , Tingting Shan , Yang Chen , Hongyi Wen , Mengshi Lin , Zhongqi Fan , Hui Wang , Yihui Chen , Hetong Lin , Yifen Lin","doi":"10.1016/j.postharvbio.2024.113258","DOIUrl":"10.1016/j.postharvbio.2024.113258","url":null,"abstract":"<div><div>This work investigated how salicylic acid (SA) treatment enhanced the storage quality of longan fruit by affecting its respiration and energy metabolism. Compared to control longan, SA-treated longan exhibited higher levels of commercially acceptable fruit rate, sucrose, vitamin C, TSS, and total soluble sugar, but lower levels of fruit disease index, pulp breakdown index, weight loss, TA, and reducing sugar. Additionally, SA treatment resulted in lower fruit respiration rate, lower activities of the critical enzymes involved in respiratory pathways (PGI, SDH, CCO, AAO), lower values of NADH and NAD, higher activities of enzymes (NADK, G-6-PDH + 6-PGDH), and higher amounts of NADPH and NADP. Besides, SA-treated longan showed higher levels of ATP, ADP, and energy charge, but lower AMP level, along with higher activities of H<sup>+</sup>-ATPase, Ca<sup>2+</sup>-ATPase, and Mg<sup>2+</sup>-ATPase in mitochondrial, plasma, and vacuolar membranes. These data indicated that SA treatment-maintained the storage attributes of postharvest longan was due to the weakened respiratory intensity, the reduced consumption of nutrients, the inhibited respiratory pathways of EMP-TCA cycle, and the enhanced respiratory pathway of PPP. Additionally, SA treatment retained higher levels of ATPase activity and energy status to maintain cellular ionic balance and protect membrane integrity, ultimately improved the storability of fresh longan during storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113258"},"PeriodicalIF":6.4,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xueli Shang , Shiqi Bai , Liang Wen , Alfred Mugambi Mariga , Ning Ma , Donglu Fang , Wenjian Yang , Qiuhui Hu , Fei Pei
{"title":"UV-B radiation mitigates oxidative stress damage in postharvest Agaricus bisporus by modulating the antioxidant defense system","authors":"Xueli Shang , Shiqi Bai , Liang Wen , Alfred Mugambi Mariga , Ning Ma , Donglu Fang , Wenjian Yang , Qiuhui Hu , Fei Pei","doi":"10.1016/j.postharvbio.2024.113266","DOIUrl":"10.1016/j.postharvbio.2024.113266","url":null,"abstract":"<div><div><em>Agaricus bisporus</em> (<em>A. bisporus</em>) has fragile tissues and is highly susceptible to post-harvest decay and spoilage, which affecting the development of the industry. Ultraviolet B (UV-B) irradiation, as a typical irradiation preservation technology, is effective in inducing the production of endogenous metabolic substances in organisms and enhancing their level of resistance. The objective of this study was to investigate the mechanism of activation of the antioxidant defence system in <em>A. bisporus</em> by UV-B irradiation, utilising a range of UV-B irradiation doses (0, 25, 50 and 100 kJ m<sup>−2</sup>). In this study we found that 50 kJ m<sup>−2</sup> UV-B irradiation effectively delayed the accumulation of reactive oxygen species (ROS), inhibited NADPH oxidase (NOX) activity and the expression of <em>Rbohf</em>, <em>PXMP2</em>, <em>PXMP4</em>, <em>APO</em>, and <em>MPV17</em>. Moreover, it could increase the accumulation of ascorbate (AsA) and glutathione (GSH), enhance the activities of ascorbate peroxidase (APX) and glutathione peroxidase (GSH-PX), and it also effectively induce catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD) activities and up-regulate the expression levels of related genes. In addition, we found that UV-B irradiation upregulated the expression of <em>UVR8</em> and suppressed the expression of <em>PEX5</em>, <em>PEX11</em> and <em>PMD1</em>. These results suggest that 50 kJ m<sup>−2</sup> UV-B irradiation could stimulate the UV Resistance Loucs 8 (UVR8) receptor, regulate peroxisome proliferation, and enhance the ability of <em>A. bisporus</em> to resist oxidative stress, thereby maintaining the cellular redox homeostasis, this provides a new strategy for the study of extended postharvest storage stability of <em>A. bisporus</em>.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113266"},"PeriodicalIF":6.4,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pre-storage anoxia treatment induces physicochemical characteristics of ‘Sucrier’ bananas (Musa AA group) in response to postharvest cold storage and subsequent ambient temperature","authors":"Wasetthee Sittiprasert , Suriyan Supapvanich , Sirima Sinthusamran , Hataitip Nimitkeatkai , Chairat Techavuthiporn","doi":"10.1016/j.postharvbio.2024.113264","DOIUrl":"10.1016/j.postharvbio.2024.113264","url":null,"abstract":"<div><div>The rapid ripening and senescence are the main problems of ‘Surcrier’ bananas during marketing. This study examined the impact of a short-term anoxia treatment on the physicochemical characteristics of ‘Sucrier’ bananas during refrigeration (15 °C), followed by exposure to 25 °C for 2 days. The anoxia treatment delayed the increases in the weight loss, and pulp-to-peel ratio as well as the pulp softening during refrigeration and after shifting to 25 °C compared to the untreated bananas. Moreover, at the end of refrigeration, the yellow color value (YCV) was 0.149 for control and 0.122 for anoxia-treated fruits. Meanwhile, on day 21+2, the YCV values increased to 0.246 for control and 0.275 for anoxia-treated fruits. Electrolyte leakage, malondialdehyde, and hydrogen peroxide levels were found to be reduced by the increased capacity of 2,2-diphenyl-2-picrylhydrazyl (DPPH) scavenging in the peel. This suggests that anoxia treatment may be able to mitigate physiological damage and senescent spotting in ‘Sucrier’ bananas during cold storage and subsequent ambient temperature. The total soluble solids (TSS) content in control fruit increased from 5.25 % to 22.00 % after storage, while anoxia-treated fruit exhibited an increase in TSS content to 18.67 %, resulting in a delay in fruit ripening. In addition, the investigation revealed that the treatment of anoxia improved the antioxidant capacity of banana pulp by augmenting the total phenolic content and DPPH scavenging in the pulp. According to the results of this study, it is most beneficial to employ anoxic treatment prior to storage in terms of both fruit ripening and quality considerations.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113264"},"PeriodicalIF":6.4,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of tropolone on gray mold rot in harvested fruit and its involved mechanism","authors":"Daoguo Chen , Yongxian Chen , Tong Chen , Yong Chen , Yong Xu , Andreas Blennow , Boqiang Li , Shiping Tian","doi":"10.1016/j.postharvbio.2024.113255","DOIUrl":"10.1016/j.postharvbio.2024.113255","url":null,"abstract":"<div><div><em>Botrytis cinerea</em> is one of the most devastating pathogen responsible for postharvest decay of fresh fruits and vegetables. However, traditional fungicide application is inclined to develop resistance strains, complicating the control of gray mold. Hence, there is an urgent need for exploiting new antifungal active substances and enhancing their antifungal activity in an environmentally friendly manner. In this study, tropolone was demonstrated efficacy in inhibiting <em>B. cinerea</em> mycelial growth at relatively low (40 mg L<sup>−1</sup>) tropolone concentrations and spore germination at even lower (10 mg L<sup>−1</sup>) tropolone concentrations. The antifungal mechanisms were linked to the disruption of spore germination-related gene expression rather than directly killing <em>B. cinerea</em> through cell membrane disruption. The inhibition of spore germination hindered the penetration of <em>B. cinerea</em> into host cells, thereby reducing disease severity in harvested apples and strawberries. To increase the protective effect of tropolone, we prepared small-sized maize starch nanoparticles loaded with tropolone and confirmed an enhanced antifungal effect. This study provides a theoretical foundation for the application of small-sized maize starch nanoparticles to increase antifungal effect and reduce fungicide application in postharvest disease control.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113255"},"PeriodicalIF":6.4,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}