Breath of the chill: Transcriptomic exploration of respiration and energy metabolism in sweet potato roots during cold storage

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-02-26 DOI:10.1016/j.postharvbio.2025.113483

Wanying Ge, Qian Zhou, Zhuohui Meng, Jihui Gao, Gang Chen, Yongxin Li, Huqing Yang

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引用次数: 0

Abstract

Chilling injury (CI) significantly affects the postharvest storage of sweet potato (Ipomoea batatas Lam.), leading to quality deterioration and substantial economic losses. This study aimed to elucidate the molecular basis of CI with a focus on respiration and energy metabolism under cold storage. Physiological and enzymatic analyses revealed disruptions in energy homeostasis and metabolic adjustments, including altered activities of respiratory enzymes and increased reliance on alternative respiration pathway. RNA-seq analysis identified differentially expressed genes (DEGs) involved in key metabolic pathways, including mitochondrial respiration, photosynthesis suppression, and proteasome-mediated protein turnover. Weighted gene co-expression network analysis (WGCNA) and gene set enrichment analysis (GSEA) highlighted transcriptional networks and hub genes, including aquaporin PIP2–7, anther specific proteins, VQ protein, and a NF-YB transcription factor, associated with CI progression. These data provide promising targets for developing molecular strategies to improve cold tolerance, thereby extending the shelf life of sweet potatoes and minimizing postharvest losses.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.