s.s erevisiae NX2320启动JA/ET信号和苯丙素代谢，增强葡萄果实对葡萄灰霉病的抗性：一项综合转录组-代谢组研究

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-10-16 DOI:10.1016/j.postharvbio.2025.113997

Xiaohua Ma , Jie Guo , Siheng Shen , Deming Liu , Yanlin Liu , Xiaoming Chen , Xixi Zhao

{"title":"s.s erevisiae NX2320启动JA/ET信号和苯丙素代谢，增强葡萄果实对葡萄灰霉病的抗性：一项综合转录组-代谢组研究","authors":"Xiaohua Ma , Jie Guo , Siheng Shen , Deming Liu , Yanlin Liu , Xiaoming Chen , Xixi Zhao","doi":"10.1016/j.postharvbio.2025.113997","DOIUrl":null,"url":null,"abstract":"<div><div>Gray mold caused by <em>Botrytis cinerea</em> is one of the major postharvest diseases of fruits during storage and transportation, and traditional chemical control faces challenges such as pesticide residues and the development of pathogen resistance. As a safe antagonistic yeast, <em>S. cerevisiae</em> NX2320 shows promising potential for biological control. In this study, various fruits such as apple, apricot, yellow peach, Shine-Muscat grape, Red Globe grape, and tomato were used as a model to systematically evaluate the inhibitory effect of <em>S. cerevisiae</em> NX2320 on gray mold and its impact on fruit quality, the molecular mechanism of its induced resistance in Red Globe grape was elucidated through integrated transcriptomic and metabolomic analyses. Results showed that yeast treatment reduced fruit decay incidence after 6 days of storage and significantly maintained higher vitamin C levels, titratable acidity, and firmness. <em>S. cerevisiae</em> NX2320 rapidly colonized the fruit surface and markedly enhanced the activities of antioxidant-related parameters such as peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), while reducing malondialdehyde (MDA) levels. Transcriptome analysis identified 2473 differentially expressed genes, with transcription factors (TFs) including <em>WRKY</em>, <em>ERF</em>, and <em>MYB</em> significantly upregulated and enriched in plant hormone signaling and phenylpropanoid biosynthesis pathways. Metabolomic analysis revealed 138 differential metabolites, including defense-related phenolic and flavonoid compounds such as resveratrol and polydatin. Integrated multi-omics analysis demonstrated that <em>S. cerevisiae</em> NX2320 activates a systemic resistance network by coordinating ROS signaling, hormonal regulation, and secondary metabolic reprogramming. This study provides theoretical insights and potential microbial resources for the green postharvest management of grape diseases.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"232 ","pages":"Article 113997"},"PeriodicalIF":6.8000,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"S.cerevisiae NX2320 primes JA/ET signaling and phenylpropanoid metabolism to enhance postharvest resistance of grape fruit against Botrytis cinerea: An integrated transcriptome-metabolome study\",\"authors\":\"Xiaohua Ma , Jie Guo , Siheng Shen , Deming Liu , Yanlin Liu , Xiaoming Chen , Xixi Zhao\",\"doi\":\"10.1016/j.postharvbio.2025.113997\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Gray mold caused by <em>Botrytis cinerea</em> is one of the major postharvest diseases of fruits during storage and transportation, and traditional chemical control faces challenges such as pesticide residues and the development of pathogen resistance. As a safe antagonistic yeast, <em>S. cerevisiae</em> NX2320 shows promising potential for biological control. In this study, various fruits such as apple, apricot, yellow peach, Shine-Muscat grape, Red Globe grape, and tomato were used as a model to systematically evaluate the inhibitory effect of <em>S. cerevisiae</em> NX2320 on gray mold and its impact on fruit quality, the molecular mechanism of its induced resistance in Red Globe grape was elucidated through integrated transcriptomic and metabolomic analyses. Results showed that yeast treatment reduced fruit decay incidence after 6 days of storage and significantly maintained higher vitamin C levels, titratable acidity, and firmness. <em>S. cerevisiae</em> NX2320 rapidly colonized the fruit surface and markedly enhanced the activities of antioxidant-related parameters such as peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), while reducing malondialdehyde (MDA) levels. Transcriptome analysis identified 2473 differentially expressed genes, with transcription factors (TFs) including <em>WRKY</em>, <em>ERF</em>, and <em>MYB</em> significantly upregulated and enriched in plant hormone signaling and phenylpropanoid biosynthesis pathways. Metabolomic analysis revealed 138 differential metabolites, including defense-related phenolic and flavonoid compounds such as resveratrol and polydatin. Integrated multi-omics analysis demonstrated that <em>S. cerevisiae</em> NX2320 activates a systemic resistance network by coordinating ROS signaling, hormonal regulation, and secondary metabolic reprogramming. This study provides theoretical insights and potential microbial resources for the green postharvest management of grape diseases.</div></div>\",\"PeriodicalId\":20328,\"journal\":{\"name\":\"Postharvest Biology and Technology\",\"volume\":\"232 \",\"pages\":\"Article 113997\"},\"PeriodicalIF\":6.8000,\"publicationDate\":\"2025-10-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Postharvest Biology and Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S092552142500609X\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Postharvest Biology and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S092552142500609X","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

摘要

灰霉病是水果储运过程中主要的采后病害之一，传统的化学防治面临着农药残留和病原菌抗性发展等挑战。作为一种安全的拮抗酵母，酿酒酵母NX2320具有良好的生物防治潜力。本研究以苹果、杏、黄桃、马斯斯特葡萄、红地球葡萄和番茄等多种水果为模型，系统评价了酿酒酵母NX2320对灰霉菌的抑制作用及其对果实品质的影响，并通过转录组学和代谢组学综合分析阐明了其诱导红地球葡萄抗灰霉菌的分子机制。结果表明，酵母处理降低了贮藏6天后果实腐烂的发生率，并显著保持了较高的维生素C水平、可滴定酸度和硬度。葡萄球菌NX2320能快速定殖，显著提高果实表面过氧化氢酶（POD）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）等抗氧化相关参数的活性，同时降低丙二醛（MDA）水平。转录组分析鉴定出2473个差异表达基因，其中WRKY、ERF和MYB等转录因子在植物激素信号通路和苯丙类生物合成途径中显著上调和富集。代谢组学分析发现了138种差异代谢物，包括与防御相关的酚类化合物和类黄酮化合物，如白藜芦醇和多柚素。综合多组学分析表明，S. cerevisiae NX2320通过协调ROS信号、激素调节和次生代谢重编程激活系统性抗性网络。本研究为葡萄绿色采后病害管理提供了理论见解和潜在的微生物资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

S.cerevisiae NX2320 primes JA/ET signaling and phenylpropanoid metabolism to enhance postharvest resistance of grape fruit against Botrytis cinerea: An integrated transcriptome-metabolome study

Gray mold caused by Botrytis cinerea is one of the major postharvest diseases of fruits during storage and transportation, and traditional chemical control faces challenges such as pesticide residues and the development of pathogen resistance. As a safe antagonistic yeast, S. cerevisiae NX2320 shows promising potential for biological control. In this study, various fruits such as apple, apricot, yellow peach, Shine-Muscat grape, Red Globe grape, and tomato were used as a model to systematically evaluate the inhibitory effect of S. cerevisiae NX2320 on gray mold and its impact on fruit quality, the molecular mechanism of its induced resistance in Red Globe grape was elucidated through integrated transcriptomic and metabolomic analyses. Results showed that yeast treatment reduced fruit decay incidence after 6 days of storage and significantly maintained higher vitamin C levels, titratable acidity, and firmness. S. cerevisiae NX2320 rapidly colonized the fruit surface and markedly enhanced the activities of antioxidant-related parameters such as peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), while reducing malondialdehyde (MDA) levels. Transcriptome analysis identified 2473 differentially expressed genes, with transcription factors (TFs) including WRKY, ERF, and MYB significantly upregulated and enriched in plant hormone signaling and phenylpropanoid biosynthesis pathways. Metabolomic analysis revealed 138 differential metabolites, including defense-related phenolic and flavonoid compounds such as resveratrol and polydatin. Integrated multi-omics analysis demonstrated that S. cerevisiae NX2320 activates a systemic resistance network by coordinating ROS signaling, hormonal regulation, and secondary metabolic reprogramming. This study provides theoretical insights and potential microbial resources for the green postharvest management of grape diseases.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.