Chondroitin sulfate alleviates leaf senescence of Chinese flowering cabbage by modulating homeostasis of ROS and chlorophyll catabolism during storage

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-02-20 DOI:10.1016/j.postharvbio.2025.113463

Pengfei Du , Yaqi Li , Yulong Chen , Hua Huang , Fanwei Dai , Jianye Chen , Mingqiang Ye , Feiping Chen , Yingwei Qi , Zheng Luo , Ling Wang

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引用次数: 0

Abstract

Chinese flowering cabbage is a nutrient abundance and popular vegetable while being sensitive to leaf senescence after harvest. Herein, the application of chondroitin sulfate (CS), a glycosaminoglycan, to delay the postharvest leaf senescence of Chinese flowering cabbage were comprehensively investigated. CS treatment could effectively inhibit the changes of yellowing index, chromaticity values of L* , C* , hue angle, and decrease of chlorophyll content in leaf of Chinese flowering cabbage. The expression levels of chlorophyll degradation catabolic genes BrNYC1, BrSGR1, BrSGR2, BrPPH, BrPAO, and BrRCCR, and representative senescence associated genes BrSAG12 and BrSAG21 were evidently repressed by CS as compared to control. The treatment of CS slowed down the production of the superoxide radical (O₂^-.), hydrogen peroxide (H₂O₂) and malondialdehyde. The activity enzyme of nicotinamide adenine dinucleotide phosphate oxidase (NOX), and the transcript abundance of respiratory burst oxidase homologues (RBOH) genes of BrRbohB, BrRbohC, BrRbohD, BrRbohD2 and BrRbohE were retarded. Additionally, the activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) was maintained, and their corresponding genes BrCAT3, BrCu/Zn-SOD1, BrPOD42, and BrAPX5 were up-regulated. These findings indicate that application of CS might potentially delay the leaf senescence by modulating ROS homeostasis and chlorophyll degradation for Chinese flowering cabbage during storage.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.