Jasmonic acid signaling pathway is involved in agaro-oligosaccharides-induced disease resistance of peach fruit through PpMYC2-activated transcription of PpPGIP1

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-02-24 DOI:10.1016/j.postharvbio.2025.113477

Qian Li , Yingying Wei , Shu Jiang , Jianfen Ye , Yi Chen , Feng Xu , Yongjiang Lou , Phebe Ding , Xingfeng Shao

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引用次数: 0

Abstract

Agaro-oligosaccharides (AO) can enhance peach fruit resistance to Monilinia fructicola and reduce postharvest decay, but its molecular mechanism is unclear. In this study, transcriptome analysis and RT-qPCR analysis revealed that AO activated the expression of genes associated with jasmonic acid (JA) synthesis (PpLOX5–1/PpLOX5–2/PpAOC/PpAOS/PpOPR2) and signal transduction (PpMYC2/PpJAZ5), along with a rise in JA content. Additionally, AO significantly up-regulated the expression of the gene encoding polygalacturonase-inhibiting protein 1 (PpPGIP1) participating in plant-pathogen interaction. Yeast one-hybrid assay and electrophoretic mobility shift assay revealed that PpMYC2 bound to the PpPGIP1 promoter through the G-box element. Dual luciferase reporter assay in tobacco, as well as transient overexpression and silencing of PpMYC2 in peach fruit, demonstrated that PpMYC2 positively regulated PpPGIP1 expression. In summary, our findings indicated that AO activated JA synthesis and signal transduction, subsequently triggering PpMYC2 to positively regulate PpPGIP1 expression, thereby boosting the resistance of peach fruit to M. fructicola infection.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.