Plant Science最新文献_第4页

Complementation with TaNCL2-A reinstates growth and abiotic stress response in atncl mutant of Arabidopsis

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-04 DOI: 10.1016/j.plantsci.2025.112411

Ishu, Shumayla, Madhu, Santosh Kumar Upadhyay

{"title":"Complementation with TaNCL2-A reinstates growth and abiotic stress response in atncl mutant of Arabidopsis","authors":"Ishu, Shumayla, Madhu, Santosh Kumar Upadhyay","doi":"10.1016/j.plantsci.2025.112411","DOIUrl":"10.1016/j.plantsci.2025.112411","url":null,"abstract":"<div><div>The sodium/calcium exchanger-like (NCL) transporters are members of Ca<sup>2 +</sup>/Cation antiporters (CaCAs) family, localized at the tonoplast, and primarily involved in Ca<sup>2+</sup> homeostasis and stress response. They transport Ca<sup>2+</sup> to the cytosol and sequester cytosolic Na<sup>+</sup> into the vacuole. Therefore, the <em>atncl</em> mutant of <em>Arabidopsis thaliana</em> is prone to salinity stress. The functional complementation of <em>TaNCL2-A</em> of <em>Triticum aestivum</em> improved abiotic stress response and various morpho-physio-biochemical parameters in <em>atncl</em> mutant. The <em>TaNCL2-A</em> complementation increased the seed germination rate and root length of <em>atncl</em> mutant during salinity and drought stress conditions. The exogenous Ca<sup>2+</sup> application further improved the stress tolerance in the complemented lines. The results suggested that the modulation of cytosolic Ca<sup>2+</sup> by <em>TaNCL2-A</em> expression and/or exogenous Ca<sup>2+</sup> application could reinstate growth and abiotic stress response in <em>atncl</em> mutant. <em>TaNCL2-A</em> also reduced the impact of ABA on seed germination. In addition, exogenous IAA induced lateral roots formation in all the lines. Biochemical and physiological analyses revealed increased proline, chlorophylls, carotenoids and relative water content (RWC), and reduced malondialdehyde (MDA), H<sub>2</sub>O<sub>2</sub> and relative electrical conductivity (REC) in <em>TaNCL2-A</em> complemented lines. The results highlighted the function of <em>TaNCL2-A</em> gene in stress response, and its potential application in crop improvement strategies in future studies.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112411"},"PeriodicalIF":4.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic insights into DXO1 and XRN3: regulatory roles of RNA stability, transcription, and liquid-liquid phase separation in Arabidopsis thaliana (L.) Heynh.

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-03 DOI: 10.1016/j.plantsci.2025.112413

Mostafakamal Shams , Ali Khadivi

{"title":"Mechanistic insights into DXO1 and XRN3: regulatory roles of RNA stability, transcription, and liquid-liquid phase separation in Arabidopsis thaliana (L.) Heynh.","authors":"Mostafakamal Shams , Ali Khadivi","doi":"10.1016/j.plantsci.2025.112413","DOIUrl":"10.1016/j.plantsci.2025.112413","url":null,"abstract":"<div><div>The regulation of RNA stability and transcription in eukaryotic organisms is a sophisticated process involving various complex mechanisms. This paper explores the regulatory functions of DXO1 and XRN3 proteins in RNA stability and transcription in the model plant <em>Arabidopsis thaliana</em> (L.) Heynh. DXO1 is noted for its roles in mRNA 5′-end quality control, removal of non-canonical NAD<sup>+</sup> caps, and activation of RNA guanosine-7 methyltransferase. In contrast, XRN3 ensures RNA integrity through precise degradation. While current studies have identified various termination regions across genes influenced by XRN3, advanced RNA sequencing techniques have revealed that XRN3-mediated changes in gene expression often result from siRNA production, leading to gene silencing rather than direct effects on transcription termination. This review emphasizes the need to further explore the DXO1-XRN3 axis, their interactive mechanisms, and their potential involvement in liquid-liquid phase separation (LLPS) during transcription. It further suggests evaluating XRN proteins like XRN4 to assess potential redundancies in RNA degradation pathways. The advent of PSPredictor, a tool for identifying LLPS proteins, along with protein function prediction techniques, promises to advance our understanding of DXO1 and XRN3 in maintaining RNA equilibrium and the dynamics of LLPS in plant biology. The review concludes by calling for more studies on the plant-specific roles of the DXO1 N-terminal extension (NTE), predictive tools for LLPS-forming proteins, and the interplay of RNA Pol II CTD code modulation by transcription factors to enhance knowledge of plant stress adaptation and improve agricultural productivity.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112413"},"PeriodicalIF":4.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of nitric oxide and nitrogen in mediating copper stress in Brassica juncea L.

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-03 DOI: 10.1016/j.plantsci.2025.112414

Bilal A. Rather , Asim Masood , Fei Qiao , Xuefei Jiang , Muhammad Mubashar Zafar , Hanqing Cong , Nafees A. Khan

{"title":"The role of nitric oxide and nitrogen in mediating copper stress in Brassica juncea L.","authors":"Bilal A. Rather , Asim Masood , Fei Qiao , Xuefei Jiang , Muhammad Mubashar Zafar , Hanqing Cong , Nafees A. Khan","doi":"10.1016/j.plantsci.2025.112414","DOIUrl":"10.1016/j.plantsci.2025.112414","url":null,"abstract":"<div><div>Copper (Cu) holds a significant importance in plant metabolism as it serves as an essential micronutrient but becomes toxic at higher concentrations. Nitric oxide (NO), a key signaling molecule, and nitrogen (N) play essential roles in combating toxicity of some metals. This study explores the potential of interactive effects of NO as 100 µM SNP (sodium nitroprusside, NO source) and N (80 mg N kg<sup>−1</sup> soil) in mitigating Cu (100 mg Cu kg<sup>−1</sup> soil) stress in mustard (<em>Brassica juncea</em> L.) plants. The impaired physio-biochemical changes, photosynthetic efficiency, and the expression level of genes associated with photosynthesis, and N assimilation under Cu stress were ameliorated with the exogenous application of NO and N. The combined treatment of NO and N conspicuously lowered reactive oxygen species (ROS) and its related impacts. It also enhanced the activity and relative expression of antioxidant enzymes, including ascorbate peroxidase (APX), glutathione reductase (GR), and superoxide dismutase (SOD) as well as N assimilation enzymes, such as nitrate reductase (NR) and nitrite reductase (NiR). The supplementation of NO and N also triggered the expression of <em>rbcL</em> (large subunit of Rubisco), photosystem (photosystem II D1 protein<em>; psbA</em> and photosystem II protein B; <em>psbB</em>) and markedly improved photosynthetic capacity under Cu stress. The study highlights the significance of NO and N as a potential strategy to counteract Cu-induced stress in crops. It suggests a synergistic or interactive effect between the two substances as a phytoremediation strategy for enhancing crop growth and productivity in Cu-contaminated soils. Understanding the mechanisms behind NO and N mediated stress alleviation could facilitate the development of targeted approaches to enhance plant resilience against heavy metal stress.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112414"},"PeriodicalIF":4.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tolerance of Oryza sativa to low phosphate is associated with adaptive changes in root architecture and metabolic exudates

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-03 DOI: 10.1016/j.plantsci.2025.112415

Akanksha Srivastava , Amber Gupta , Sujit K. Bishi , Pole Akhila , P.C. Latha , D. Subrahmanyam , P. Brajendra , M.S. Anantha , Suvarna Rani Ch , Akshay S. Sakhare , Vijai Pal Bhadana , Jitender Giri , C.N. Neeraja , R.M. Sundaram , Satendra K. Mangrauthia

{"title":"Tolerance of Oryza sativa to low phosphate is associated with adaptive changes in root architecture and metabolic exudates","authors":"Akanksha Srivastava , Amber Gupta , Sujit K. Bishi , Pole Akhila , P.C. Latha , D. Subrahmanyam , P. Brajendra , M.S. Anantha , Suvarna Rani Ch , Akshay S. Sakhare , Vijai Pal Bhadana , Jitender Giri , C.N. Neeraja , R.M. Sundaram , Satendra K. Mangrauthia","doi":"10.1016/j.plantsci.2025.112415","DOIUrl":"10.1016/j.plantsci.2025.112415","url":null,"abstract":"<div><div>The optimum usage of fertilizers is key for the sustainable agriculture. Among nutrients, phosphorus (P) is critical for plant growth and development. Due to complete reliance on natural resources (rock phosphate) for P, the availability of P fertilizers is emerging as a global challenge for crop cultivation. Moreover, the excess application of P fertilizers in rice, mostly grown under flooded conditions, leads to water pollution called eutrophication. In this study, we employed a mutagenesis approach for developing and characterizing rice EMS (ethyl methanesulfonate) mutants with better adaptation to low soil P conditions. One such mutant of rice cultivar Nagina 22, named NH4824, was characterized comprehensively at seedling and reproductive growth stages under hydroponic and field conditions. The mutant exhibits low soil P tolerance due to combined adaptive changes in root system architecture, anatomy, organic acid exudates, plasma membrane (PM) H<sup>+</sup>-ATPase activity, induced expression of P transporter genes, and efficient mobilization and partitioning of P in different plant tissues. The activity of antioxidant enzymes and better photosynthesis suggested relatively less stress experienced by NH4824 than N22 under low soil P conditions. These insights are highly useful to develop P use efficient crop cultivars through breeding or genome editing approaches.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112415"},"PeriodicalIF":4.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A critical review of the importance of Far-Related Sequence (FRS)- FRS-Related Factor (FRF) transcription factors in plants

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-01 DOI: 10.1016/j.plantsci.2025.112410

Fereshteh Jafari , Aria Dolatabadian

{"title":"A critical review of the importance of Far-Related Sequence (FRS)- FRS-Related Factor (FRF) transcription factors in plants","authors":"Fereshteh Jafari , Aria Dolatabadian","doi":"10.1016/j.plantsci.2025.112410","DOIUrl":"10.1016/j.plantsci.2025.112410","url":null,"abstract":"<div><div>Transposable elements have long been recognised as critical drivers of genetic diversity and evolution in plant genomes, influencing various physiological and developmental processes. The transcription factor family FAR-RED ELONGATED HYPOCOTYLS3 (FHY3), and its homologue FAR-RED IMPAIRED RESPONSE1 (FAR1), initially identified as key components of phytochrome A (phyA)-mediated far-red (FR) light signalling in <em>Arabidopsis thaliana</em>, are derived from transposases and are essential for light signal transduction, plant growth, and development. FHY3 and FAR1 are also the founding members of the FAR1-RELATED SEQUENCE (FRS) family, which is conserved across terrestrial plants. While the coding sequences of many putative FRS and FAR1-RELATED FACTOR (FRF) orthologs have been identified in various angiosperm clades, their physiological functions remain largely unexplored. The FRF genes are considered truncated forms of FRS proteins that compete with FRS for DNA binding sites, thereby regulating gene expression.</div><div>This review highlights recent advances in characterising the molecular mechanisms of FHY3, FAR1, and other members of the FRS-FRF protein family. We examine their roles in key processes such as regulating flowering time, controlling branching, integrating leaf aging and senescence, modulating the circadian clock, maintaining meristem function, starch synthesis, seed germination, and responding to Starch synthesis and carbon starvation. Additionally, we explore their contributions to plant immunity under biotic and abiotic stresses. Finally, we suggest future directions for functional characterising other FRS-FRF family proteins in plants, which could provide deeper insights into their regulatory roles in plant biology.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112410"},"PeriodicalIF":4.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sugar and anthocyanins: A scientific exploration of sweet signals and natural pigments

IF 4.2 2区生物学

Plant Science Pub Date : 2025-01-31 DOI: 10.1016/j.plantsci.2025.112409

Ting Zhao , Qian Li , Ting Yan , Boping Yu , Qi Wang , Delu Wang

{"title":"Sugar and anthocyanins: A scientific exploration of sweet signals and natural pigments","authors":"Ting Zhao , Qian Li , Ting Yan , Boping Yu , Qi Wang , Delu Wang","doi":"10.1016/j.plantsci.2025.112409","DOIUrl":"10.1016/j.plantsci.2025.112409","url":null,"abstract":"<div><div>The presence of anthocyanins imparts vibrant hues to plants, whose biosynthesis and accumulation is a complex process and are influenced by numerous factors. In plants, sugar acts as a primary energy source and signaling molecule regulating anthocyanins biosynthesis. In this review, we provides a comprehensive overview of the relationship between sugar and anthocyanin. We delved into the intricate biosynthetic pathway of anthocyanins, outlining the key structural genes involved and their functions. Furthermore, we summarized how various environmental factors such as sugar, light, abiotic stresses, etc., affect anthocyanin biosynthesis. Notably, Most notably, we emphasized that sugars can independently regulate anthocyanin biosynthesis by modulating the expression of the MBW complex or structural genes, as well as through cascades involving hormones. These findings offer valuable insights into understanding the molecular mechanisms underlying anthocyanin accumulation and present potential avenues for enhancing anthocyanin content in plants through targeted manipulations that could have applications in agriculture and nutrition.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112409"},"PeriodicalIF":4.2,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of the interaction mechanisms between jasmonic acid (JA) and various plant hormones, as well as the core regulatory role of MYC2

IF 4.2 2区生物学

Plant Science Pub Date : 2025-01-31 DOI: 10.1016/j.plantsci.2025.112407

Tingting Mu , Shilei Luo , Long Li , Rongrong Zhang , Peng Wang , Guobin Zhang

引用次数: 0

Unveiling the novel role of spermidine in leaf senescence: A study of eukaryotic translation factor 5A-independent and dependent mechanisms

IF 4.2 2区生物学

Plant Science Pub Date : 2025-01-31 DOI: 10.1016/j.plantsci.2025.112408

Ewelina Paluch-Lubawa , Kinga Popławska , Magdalena Arasimowicz-Jelonek , Ewa Sobieszczuk-Nowicka

{"title":"Unveiling the novel role of spermidine in leaf senescence: A study of eukaryotic translation factor 5A-independent and dependent mechanisms","authors":"Ewelina Paluch-Lubawa , Kinga Popławska , Magdalena Arasimowicz-Jelonek , Ewa Sobieszczuk-Nowicka","doi":"10.1016/j.plantsci.2025.112408","DOIUrl":"10.1016/j.plantsci.2025.112408","url":null,"abstract":"<div><div>Senescence is a crucial and highly active process in plants, optimising resource allocation and promoting phenotypic plasticity under restricted conditions. It involves global metabolic reprogramming for the organised disintegration and remobilization of resources. Polyamines (PAs) are polycationic biogenic amines prevalent in all eukaryotes and are necessary for cell survival. The commonly used PAs in plants include putrescine, spermidine, and spermine. Notably, the leaf's expression of S-adenosylmethionine decarboxylase and spermidine synthase gene family transcripts significantly changes during senescence. This suggests these genes are critical in spermidine metabolism and may condition metabolic reprogramming. One key role of spermidine in eukaryotes is to provide the 4-aminobutyl group for the posttranslational modification of lysine in eukaryotic translation factor 5A (eIF5A). This modification is catalysed by two sequential enzymatic steps leading to the activation of eIF5A by converting lysine to the unusual amino acid hypusine. Although eIF5A is well characterised to be involved in the translation of proline-rich repeat proteins and other hard-to-read motifs, the biological role of eIF5A has recently been clarified only in mammals. It could be better described at the plant functional level. The expression patterns of <em>eIF5A</em> isoforms and genes encoding machinery responsible for hypusination, differ between induced and developmental leaf senescence. In this paper, we summarise the existing knowledge on spermidine-dependent senescence control mechanisms in plants, raising the possibility that spermidine could be an element of a biological switch controlling the onset of a different type of senescence in an eIF5A-independent and dependent manner.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112408"},"PeriodicalIF":4.2,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accumulation of theanine in tea plant (Camellia sinensis (L.) O. Kuntze): Biosynthesis, transportation and strategy for improvement

IF 4.2 2区生物学

Plant Science Pub Date : 2025-01-31 DOI: 10.1016/j.plantsci.2025.112406

Qianting Luo , Hua-Feng He

{"title":"Accumulation of theanine in tea plant (Camellia sinensis (L.) O. Kuntze): Biosynthesis, transportation and strategy for improvement","authors":"Qianting Luo , Hua-Feng He","doi":"10.1016/j.plantsci.2025.112406","DOIUrl":"10.1016/j.plantsci.2025.112406","url":null,"abstract":"<div><div>Theanine, specifically biosynthesized and accumulated in <em>Camellia sinensis</em> (L.) O. Kuntze, is widely recognized as the most positive ingredient related to the quality of tea. Therefore, genetic factors related to the biosynthesis of theanine in tea plant, CsAlaDC, CsGGTs and CsMYBs, etc., were elaborated and proved to be influential. Oppositely, TFs acting on the growth and development of tea plants, CsPIF, CsHO as well as CsGDH were demonstrated to be negative for biosynthesis of theanine. Since root is the original assembly site, transportation is indispensable for the accumulation of theanine in leaf. CsAAP7.2 was elucidated to be involved in the transportation of theanine crossing the vascular system to vegetative tissues. In order to promote the accumulation of theanine, strategies were proposed in aspect of processing, cultivation, fertilizer as well as germplasm innovation. Appropriate processing technology, scientific planting manner and fertilizer application, coupling with domestication of excellent varieties portrayed out the future orientation of theanine. Purpose of the review was to summarize advantages achieved in related to metabolism of theanine, and to motivate more intensive and more effective means to promote the accumulation of theanine in tea plant.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112406"},"PeriodicalIF":4.2,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An assay for assessing 1-aminocyclopropane-1-carboxylate malonyl (MACC) transferase (AMT) activity and its regulation by ethylene

IF 4.2 2区生物学

Plant Science Pub Date : 2025-01-30 DOI: 10.1016/j.plantsci.2025.112401

J. Pattyn , M. Geerts Danau , D. De Ruysscher , S. Roden , T. Snoekx , J. Masschelein , J. Vaughan-Hirsch , B. Van de Poel

{"title":"An assay for assessing 1-aminocyclopropane-1-carboxylate malonyl (MACC) transferase (AMT) activity and its regulation by ethylene","authors":"J. Pattyn , M. Geerts Danau , D. De Ruysscher , S. Roden , T. Snoekx , J. Masschelein , J. Vaughan-Hirsch , B. Van de Poel","doi":"10.1016/j.plantsci.2025.112401","DOIUrl":"10.1016/j.plantsci.2025.112401","url":null,"abstract":"<div><h3>Background</h3><div>N-malonyl 1-aminocyclopropane-1-carboxylic acid (MACC) is a major conjugate of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and may therefore play an important role in regulating ethylene production, as well as ethylene-independent ACC signalling. While the enzyme responsible for this derivatization, ACC malonyltransferase (AMT), has been studied in the past, its identity remains unknown. Methods to assay AMT activity are not well established, and no standardized assay has been described.</div></div><div><h3>Results</h3><div>We optimized an AMT activity assay and investigated the biological implications of AMT. This assay can be divided into three parts: total protein extraction, <em>in vitro</em> AMT activity assay, and MACC detection. For these three parts, different parameters were optimized and combined into an integrated and robust protocol. We used gas chromatography for the indirect detection of MACC, which was compared to a direct LC-MS approach, indicating that the GC-based method is a good alternative readily available to most labs studying ethylene. Next, we used this <em>in vitro</em> AMT activity assay to study the biological function of MACC formation. We observed an ontogenetic, tissue-specific and an ethylene-mediated feedback effect on AMT activity in tomato and Arabidopsis. The feedback of ethylene on AMT activity seems to be important to regulate ethylene production levels.</div></div><div><h3>Conclusions</h3><div>The optimized and robust AMT activity assay presented here will enable other plant researchers to investigate the biochemistry of the ethylene biosynthesis pathway through ACC conjugation into MACC. Our AMT activity method was deployed both in tomato and Arabidopsis, and revealed that AMT activity is tightly controlled by ethylene itself in a tissue-specific way.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112401"},"PeriodicalIF":4.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0