Plant Science最新文献

{"title":"LbMYB368 from the recretohalophyte Limonium bicolor promotes salt gland development and salinity tolerance","authors":"Jianglu Zhu ,&nbsp;Haoxuan Huang ,&nbsp;Xiaojing Xu ,&nbsp;Xi Wang ,&nbsp;Guoyong Zhu ,&nbsp;Baoshan Wang ,&nbsp;Jingwen Zhu ,&nbsp;Fang Yuan","doi":"10.1016/j.plantsci.2025.112486","DOIUrl":"10.1016/j.plantsci.2025.112486","url":null,"abstract":"<div><div>Halophytes can grow and reproduce normally in an environment containing more than 200 mM NaCl, offering untapped gene resources for improving crop salinity tolerance. As a recretohalophyte, <em>Limonium bicolor</em> can secrete excess Na⁺ through salt glands, specialized structures on the leaf and stem epidermis. Here, we identified a MYB transcription factor gene, <em>LbMYB368</em>, that is highly expressed during salt gland development. We confirmed its expression in salt glands using RNA <em>in situ</em> hybridization and a promoter reporter construct. To investigate in detail the roles of LbMYB368 in salinity tolerance, we overexpressed and knocked down the gene, via virus-induced gene silencing (VIGS), in <em>L. bicolor</em>. The transgenic <em>L. bicolor</em> overexpression lines developed more salt glands, while the VIGS plants had fewer salt glands. The salt secretion ability and salt tolerance of these plants were correlated with the changes in salt gland development, indicating that LbMYB368 plays an important role in the salt tolerance of <em>L. bicolor</em> by enhancing salt gland development and salt secretion. We also investigated the effect of <em>LbMYB368</em> on enhanced salinity tolerance when heterologously expressed in Arabidopsis to assess its potential applications in non-halophytes for future conferring salinity tolerance in crops.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112486"},"PeriodicalIF":4.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The oxidative stress 3-like protein GsOS3L, substrate of GsSnRK1, enhances salt and cadmium stresses in soybean roots","authors":"Xiaohuan Sun ,&nbsp;Haidong Bu ,&nbsp;Haoran Lu ,&nbsp;Junfeng Zhang ,&nbsp;Qiang Li ,&nbsp;Jialei Xiao ,&nbsp;Xiaodong Ding ,&nbsp;Lei Cao","doi":"10.1016/j.plantsci.2025.112483","DOIUrl":"10.1016/j.plantsci.2025.112483","url":null,"abstract":"<div><div>Salt and heavy metal stresses have significant impacts on crop growth and agricultural development. Wild soybean (<em>Glycine soja</em>) exhibits greater resistance to abiotic stresses than its cultivated counterpart (<em>Glycine max</em>). In this study, the oxidative stress 3-like gene GsOS3L was identified from yeast two hybridization cDNA library constructed from wild soybean RNA. 150 mM NaCl and 10 % PEG induced their expression in roots, stems and leaves, respectively. pGsOS3L:GUS activity was enhanced in various tissues with increasing NaCl and CdCl₂ concentrations. Y2H, BiFC, and LCI activity assays revealed that GsOS3L interacted physically with the GsSnRK1 kinase. The GsOS3L protein, which contains potential phosphorylation and palmitoylation sites, was localized to the nucleus under normal conditions but translocated from the nucleus to the cell membrane under cadmium stress. This translocation was prevented by the palmitoylation inhibitor 2-bromopalmitate (2-BP) and by double mutation of the predicted palmitoylation sites (C4S/C21S). 2-BP treatment attenuated GsOS3L transgenic composite soybeans’ cadmium stress tolerance. GsOS3L was found to be phosphorylated by GsSnRK1, which reduced the salt and cadmium stress tolerance of transgenic <em>Arabidopsis</em> plants. The findings of this study provide promising insights into the physiological and molecular mechanisms of GsOS3L in soybean under salt and cadmium stresses.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112483"},"PeriodicalIF":4.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strigolactone (GR24) regulates fruit ripening in yft3 tomatoes by altering ABA biosynthesis","authors":"Muhammad Naeem ,&nbsp;Rong Han ,&nbsp;Anran Xu ,&nbsp;Xuemeng Shan ,&nbsp;Lingxia Zhao","doi":"10.1016/j.plantsci.2025.112484","DOIUrl":"10.1016/j.plantsci.2025.112484","url":null,"abstract":"<div><div>Color development in tomato fruits is a key indicator of ripening, and is driven by complex hormonal and molecular interactions. The present study investigated the effects of exogenous treatment with abscisic acid (ABA), GR24 (a synthetic strigolactone analog), and water (ddH₂O as control) on tomato fruit ripening, ethylene emission, carotenoid biosynthesis, ABA metabolism, and chromoplast development in <em>yft3</em> and wild-type (WT cv<em>.</em> M82) tomato fruits at 35, 47, and 54 days post-anthesis (dpa). Results showed that GR24 significantly accelerated ripening in <em>yft3</em>, transitioning from green to deep orange at 54 dpa, whereas ABA had a moderate effect. In cv<em>.</em> M82, both treatments enhanced color development, leading to a deep red phenotype. GR24 increased ethylene emission and upregulated the expression of ethylene related genes (<em>ACO1</em>, <em>ACS2</em>/4), with <em>yft3</em> showing increased sensitivity than cv<em>.</em> M82. Carotenoid profiling revealed higher lycopene and β-carotene contents in <em>yft3</em>, with GR24 enhancing β-carotene and lutein, whereas ABA increased lycopene. GR24 and ABA strongly induced the expression of carotenoid-related genes (<em>CRTISO</em>, <em>PSY1</em>, and <em>CYCB</em>), particularly in <em>yft3</em> at 54 dpa. Moreover, ABA-content and the expression of genes involved in ABA biosynthesis (<em>NCED</em>, <em>AAO</em>), catabolism (<em>CYP707A</em>), conjugation (<em>GT</em>), and activation (<em>BG</em>) were significantly altered by ABA and GR24 treatments. Furthermore, ultrastructural analysis revealed that GR24 promoted plastoglobule formation and chromoplast differentiation, with <em>yft3</em> exhibiting a stronger response than cv<em>.</em> M82. These findings highlight that GR24 plays vital roles in regulating tomato fruit ripening, ethylene biosynthesis, carotenoids accumulation, as well as ABA-metabolism, with implications for improving fruit quality in tomatoes.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112484"},"PeriodicalIF":4.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monocot-like leaf structure and trichome-water relations in early growth stages of the C3 plant sand rice (Agriophyllum squarrosum)","authors":"Ruilan Ran ,&nbsp;Xiaofeng Li ,&nbsp;Jiwei Zhang ,&nbsp;Jiecai Zhao ,&nbsp;Xin Zhao ,&nbsp;Xiaoyun Cui ,&nbsp;Guoxiong Chen ,&nbsp;Pengshan Zhao","doi":"10.1016/j.plantsci.2025.112480","DOIUrl":"10.1016/j.plantsci.2025.112480","url":null,"abstract":"<div><div>Sand rice (<em>Agriophyllum squarrosum</em> (L.) Moq.), a species of Amaranthaceae <em>sensu lato</em>, is an annual psammophyte found mainly on sand dunes in arid and semi-arid regions of Central Asia. Trichomes on the leaf surface are a key adaptive feature of sand rice, however, their ecophysiological functions remain to be elucidated. In this study, the anatomical analysis of a leaf reveals that the foliage is characterized by its isobilateral and amphistomatic nature. Together with an average δ¹³C value of −28.57 ‰, these findings indicate that sand rice is a C₃ plant. Utilizing a trichome-defective mutant (<em>Agriophyllum squarrosum trichomeless1</em>, <em>astcl1</em>) identified from an EMS mutagenesis library by our group, alongside the wild type Shapotou (SPT), we observed that both epidermal surfaces on SPT leaves exhibit dense and dendritic trichomes, forming rough leaf boundary layers. Chlorophyll leaching analysis revealed that SPT has a higher epidermal permeability, with 86.32 % of chlorophylls leaching out within 30 minutes, compared to only 24.18 % for <em>astcl1</em>. The <em>astcl1</em> leaf displayed a higher leaf wettability, lower water loss, and thicker cuticle compared to SPT leaves, which may be associated with trichomes serving as the primary pathway for nonstomatal water movement. This is supported by the observation that toluidine blue staining is more intense in trichomes than in pavement cells. Our findings reveal that the correlation between trichomes and the cuticle appears to be a critical factor in managing water loss and overall plant hydration, highlighting the significant role of trichomes in the adaptation of sand rice to desert environments.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112480"},"PeriodicalIF":4.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological functions of the transcription factor GmZAT10-1 gene involved in the salt stress adaptation in soybean","authors":"Tianlei Zhang ,&nbsp;Li Yu ,&nbsp;Yutin Chen ,&nbsp;Yi Zeng ,&nbsp;Boyi Pi ,&nbsp;Xun Liu ,&nbsp;Bingjun Yu","doi":"10.1016/j.plantsci.2025.112485","DOIUrl":"10.1016/j.plantsci.2025.112485","url":null,"abstract":"<div><div>C2H2-type zinc finger proteins (ZFPs) play important roles in the gene transcriptional regulation in the response of plants to multiple stressful environments. In this work, the responses of the soybean ZFP family member <em>GmZAT10–1</em> gene and its promoter to salt stress, and the changes in the seedling growth phenotype, as well as the related physiological parameters in overexpressing (OE)– or CRISPR/Cas9 (KO)–<em>GmZAT10–1</em> hairy-root composite soybean seedlings and transgenic <em>Arabidopsis thaliana</em> under salt stress were investigated. The results showed that both <em>GmZAT10–1</em> and its promoter exhibited enhanced induction to salt stress, and the GmZAT10–1 protein displayed the transcriptional activation activity and was located in the cell nucleus. Transient expression of <em>GmZAT10–1</em> in tobacco leaves and yeast one-hybrid assay (Y1H) revealed that GmZAT10–1 can bind to the promoter of <em>GmCLC-c1</em> to enhance the expression of the target genes. Compared with the empty vector–transformed (Ev) hairy-root composite soybean plants, the salt-stressed OE-<em>GmZAT10–1</em> and KO-<em>GmZAT10–1</em> plants presented mitigated salt injury, greater plant height, fresh weight per plant, leaf relative water content (RWC) and chlorophyll content, and lower relative electrolytic leakage (REL) and malondialdehyde (MDA) content in the roots and leaves, among which the accumulation of Cl^– and NO₃^– increased significantly in the roots of OE-<em>GmZAT10–1</em>, which obviously reduced the transport and accumulation of Cl^– to the stems and leaves, and thus resulting in a marked decrease in Cl^–/NO₃^– ratio in the roots, stems and leaves. By introducing the <em>GmZAT10–1</em> gene into <em>A. thaliana</em> wild-type (WT) and <em>atzat10</em> mutant, the seed germination rates and root lengths of WT<em>-GmZAT10–1</em> and <em>atzat10-GmZAT10–1</em> under salt stress were obviously restored, and the leaf chlorophyll content and RWC were significantly increased, whereas the REL values and MDA contents were significantly decreased. Additionally, significant accumulation of Cl^– and Na⁺ was observed in the roots, which resulted in a significant decrease in Cl^–/NO₃^–and Na⁺/K⁺ ratios in the shoots. Taken together, these findings indicate that the transcription factor <em>GmZAT10–1</em> may confer salt tolerance in soybeans by upregulating the expression of the <em>GmCLC-c1</em> gene through binding to its promoter, regulating the uptake of Cl^– by the roots and reducing its translocation to the above-ground parts, including the stems and leaves of the plants, thereby maintaining a relatively low Cl^–/NO₃^– ratio.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112485"},"PeriodicalIF":4.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of an iron-induced enzyme, nicotianamine synthase, from giant leucaena","authors":"James T. Carrillo,&nbsp;Dulal Borthakur","doi":"10.1016/j.plantsci.2025.112481","DOIUrl":"10.1016/j.plantsci.2025.112481","url":null,"abstract":"<div><div>Metal homeostasis in giant leucaena (<em>Leucaena leucocephala</em> subsp. <em>glabrata</em>) is of interest due to the plant’s production of mimosine, an iron-chelating secondary metabolite. Real-time PCR performed on root and foliar tissue showed the upregulation of 19 genes following exogenous application of iron. Notable genes affected include glutathione synthase (20-fold increase in leaf), ferric chelate reductase (15-fold increase in root), mimosinase (20-fold increase in leaf) and nicotianamine synthase (30-fold increase in root). Transcriptome sequence data and 5’-RLM-RACE methods identified the complete nicotianamine synthase coding sequence, which was cloned for heterologous expression and in vitro assays. To properly assay nicotianamine synthase activity, due to strong feedback inhibition by 5’-methylthoadenosine, the giant leucaena 5’-methylthoadenosine nucleosidase was cloned and purified as well. Additional inhibition produced by the substrate compound, S-adenosylmethionine (SAM), was discovered in this study by utilizing a recombinant SAM-synthetase. Nicotianamine synthase is sensitive to racemic mixtures of SAM, which is inevitably produced in commercial SAM solutions. When substrate was produced in situ, using SAM-synthetase, nicotianamine synthase activity was 5-fold faster. Thus, in vitro nicotianamine synthase activity depends highly on two additional enzymes, the inclusion of MTA-nucleosidase being vital. Although promising, cell-free nicotianamine production methods are not yet efficient enough for industry-scale efforts. Sequence and structural analyses suggest residues involved in azetidine ring formation and other aspects of the mechanism are explored.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112481"},"PeriodicalIF":4.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of ZmEREB211 confers enhanced susceptibility to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis.","authors":"Bo Su, Haiyang Jiang, Zheng Song, Wenjie Liu, Shubin Rao, Hubiao Jiang, Guichun Wu, Ting Ding","doi":"10.1016/j.plantsci.2025.112482","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112482","url":null,"abstract":"<p><p>Genes in the ERF family encode trasnscripiton regulators involved in plant developmental and physiological processes. However, the function of the ERF family gene in regulation of plant susceptibility to pathogens has rarely been reported. In this study, An ERF family gene ZmEREB211 (AP2-EREBP-transcription factor 211), whose expression was significantly upregulated in response to biotic stress (Bipolaris maydis), was isolated from maize (Zea mays L.). Based on sequence homology and phylogenetic analysis, ZmEREB211 has 792bp in length and was characterized as an ERF family trasnscripiton regulator with single conserved APETALA2 (AP2) domain. Transient expression of ZmEREB211 in Nicotiana benthamiana revealed that its subcellular localization was distributed in the nucleus. Moreover, overexpression of ZmEREB211 in Arabidopsis thaliana resulted in increased susceptibility to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). Examination of disease-related physiological indicators showed that overexpression of ZmEREB211 in A. thaliana led to the accumulation of membrane lipid peroxide malondialdehyde, reduced levels of hydrogen peroxide, phenylalanine ammonia lyase (PAL) activity, and peroxidase (POD) Activity, thereby enhancing the plant susceptibility. Additionally, transcriptome and qRT-PCR data indicated that the expression of genes related to the salicylic acid (SA) pathway was suppressed upon Pst DC3000 inoculation in ZmEREB211-overexpressing A. thaliana compared with wild type, while the expression of genes related to the ethylene (ET) pathway was induced at the same time. These findings collectively suggest that the transfer of ZmEREB211 gene into A. thaliana may confer increased susceptibility to the plant by inhibiting the SA pathway and inducing ET pathway, and provide novel susceptible gene resources for crop disease resistance breeding.</p>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112482"},"PeriodicalIF":4.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycine betaine and plant abiotic stresses: Unravelling physiological and molecular responses","authors":"Nenghui Li,&nbsp;Jing Li,&nbsp;Jianming Xie,&nbsp;Wenjing Rui,&nbsp;Kaiguo Pu,&nbsp;Yanqiang Gao,&nbsp;Tiantian Wang,&nbsp;Miao Zhang","doi":"10.1016/j.plantsci.2025.112479","DOIUrl":"10.1016/j.plantsci.2025.112479","url":null,"abstract":"<div><div>Plants are constantly subjected to various abiotic stresses (drought, salinity, heavy metals and low temperature) throughout their life cycle, which significantly hinder their growth and productivity. Key abiotic stresses include drought, salinity, heavy metals, and extreme temperatures. In response, plants modulate glycine betaine (GB) levels, a vital compatible solute that influences growth and stress tolerance by interacting with phytohormones and cellular signaling pathways. Not all species can synthesize endogenous GB; however, some non-GB accumulating plants have been genetically modified to enhance GB production through the overexpression of synthesis genes such as <em>choline oxidase</em>, <em>choline monooxygenase</em>, and <em>betaine aldehyde dehydrogenase</em>. Exogenous GB treatment can mitigate stress effects by improving nutritional balance, reducing reactive oxygen species (ROS), minimizing membrane damage, and alleviating photoinhibition. Nonetheless, the specificity of GB application, transport, and accumulation across species, as well as its interaction with phytohormones in stress alleviation, remains uncertain. This review focuses on GB's role as an antioxidant, osmo-regulator, and nitrogen source, evaluating the physiological, biochemical, and molecular mechanisms by which GB mitigates abiotic stresses, aiming to develop GB-based strategies for enhancing plant stress resilience.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112479"},"PeriodicalIF":4.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The vacuole pH-related gene RcNHX2 affects flower color shift and Na+ homeostasis in roses.","authors":"Bingshuang Wang, Huanhuan Wang, Minghui Liu, Guoren He, Feng Ming","doi":"10.1016/j.plantsci.2025.112476","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112476","url":null,"abstract":"<p><p>Rose (Rosa spp.) is one of the most famous ornamental plants in the world, and its commodity value largely depends on its flower color. The color of roses mainly depends on the composition and state of anthocyanins, and the vacuolar pH value is an important factor affecting the stability and state of anthocyanins. The vacuolar sodium/proton antiporters (NHXs) play important roles in the maintenance of cellular ion homeostasis and petal vacuolar pH. However, the NHX functions related to rose flower coloration remain relatively uncharacterized. In this study, we cloned and characterized the vacuolar pH-related gene RcNHX2, which encoded a vesicular cation/H⁺ antiporter protein. Phylogenetic sequence analysis revealed that RcNHX2 belongs to the vesicular NHX family of proteins. It is localized in the vesicular membrane, where it exerts its function. RcNHX2 was significantly differentially expressed in different color-presenting types of petals of roses, and it was particularly highly expressed in the blue-purple petals. The overexpression of RcNHX2 in Rosa hybrida 'Florentina' caused the pH to increase and the petal color to change from red to blue-purple. On the basis of virus-induced gene silencing, we determined that decreased RcNHX2 expression significantly reduces R. hybrida 'Blue For You' petal coloration. We indicated that RcNHX2 might be involved in the color shift to blue in roses. Moreover, it was observed that in the cells of the rose plants in which RcNHX2 was silenced, the Na⁺ homeostasis was affected. The results suggest that the vesicular Na⁺/H⁺ transporter, RcNHX2 gene, likely plays a crucial role in the blue color change and the maintenance of cellular Na⁺ homeostasis in roses. These findings offer valuable insights for the cultivation of blue rose.</p>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112476"},"PeriodicalIF":4.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GhCTEF2 encodes a PLS-type PPR protein required for chloroplast development and plastid RNA editing in cotton","authors":"Huan He ,&nbsp;Mengxue Cheng ,&nbsp;Bowen Bao ,&nbsp;Yanan Tian ,&nbsp;Yating Zheng ,&nbsp;Yuzhu Huo ,&nbsp;Zengqiang Zhao ,&nbsp;Zongming Xie ,&nbsp;Jianing Yu ,&nbsp;Peng He","doi":"10.1016/j.plantsci.2025.112478","DOIUrl":"10.1016/j.plantsci.2025.112478","url":null,"abstract":"<div><div>Cotton is a significant cash crop and serves as a crucial raw material for the textile industry. The leaf, which is the site of photosynthesis in cotton plants, directly influences their growth and yield. Pentatricopeptide repeat (PPR) proteins are characterized by tandem 30–40 amino acid motifs. These proteins play a pivotal role in post-transcriptional regulation of organelle gene expression. In this study, we identified GhCTEF2 as a PLS-type PPR protein and determined its subcellular localization within chloroplasts, highlighting its essential involvement in chloroplast development. Virus-induced gene silencing assays revealed that knockdown of the <em>GhCTEF2</em> gene resulted in macular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency. Additionally, <em>GhCTEF2</em>-silenced plants exhibited incomplete chloroplasts with reduced thylakoids and grana structures. Furthermore, our findings showed that the downregulation of <em>GhCTEF2</em> reduced the transcription levels of PEP-dependent genes and significantly decreased the content of the chloroplast LHCⅡ-T complex protein. Further studies showed that GhCTEF2 may interact with other editing factors to regulate the RNA editing process of <em>ndhB</em>, <em>accD,</em> and <em>rps18</em>. These findings offer valuable insights into future breeding strategies aimed at enhancing photosynthesis in cotton.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112478"},"PeriodicalIF":4.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}