Plant Science最新文献

The vacuole pH-related gene RcNHX2 affects flower color shift and Na+ homeostasis in roses.

IF 4.2 2区生物学

Plant Science Pub Date : 2025-03-18 DOI: 10.1016/j.plantsci.2025.112476

Bingshuang Wang, Huanhuan Wang, Minghui Liu, Guoren He, Feng Ming

{"title":"The vacuole pH-related gene RcNHX2 affects flower color shift and Na+ homeostasis in roses.","authors":"Bingshuang Wang, Huanhuan Wang, Minghui Liu, Guoren He, Feng Ming","doi":"10.1016/j.plantsci.2025.112476","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112476","url":null,"abstract":"Rose (Rosa spp.) is one of the most famous ornamental plants in the world, and its commodity value largely depends on its flower color. The color of roses mainly depends on the composition and state of anthocyanins, and the vacuolar pH value is an important factor affecting the stability and state of anthocyanins. The vacuolar sodium/proton antiporters (NHXs) play important roles in the maintenance of cellular ion homeostasis and petal vacuolar pH. However, the NHX functions related to rose flower coloration remain relatively uncharacterized. In this study, we cloned and characterized the vacuolar pH-related gene RcNHX2, which encoded a vesicular cation/H+ antiporter protein. Phylogenetic sequence analysis revealed that RcNHX2 belongs to the vesicular NHX family of proteins. It is localized in the vesicular membrane, where it exerts its function. RcNHX2 was significantly differentially expressed in different color-presenting types of petals of roses, and it was particularly highly expressed in the blue-purple petals. The overexpression of RcNHX2 in Rosa hybrida 'Florentina' caused the pH to increase and the petal color to change from red to blue-purple. On the basis of virus-induced gene silencing, we determined that decreased RcNHX2 expression significantly reduces R. hybrida 'Blue For You' petal coloration. We indicated that RcNHX2 might be involved in the color shift to blue in roses. Moreover, it was observed that in the cells of the rose plants in which RcNHX2 was silenced, the Na+ homeostasis was affected. The results suggest that the vesicular Na+/H+ transporter, RcNHX2 gene, likely plays a crucial role in the blue color change and the maintenance of cellular Na+ homeostasis in roses. These findings offer valuable insights for the cultivation of blue rose.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112476"},"PeriodicalIF":4.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GhCTEF2 encodes a PLS-type PPR protein required for chloroplast development and plastid RNA editing in cotton.

IF 4.2 2区生物学

Plant Science Pub Date : 2025-03-17 DOI: 10.1016/j.plantsci.2025.112478

Huan He, Mengxue Cheng, Bowen Bao, Yanan Tian, Yating Zheng, Yuzhu Huo, Zengqiang Zhao, Zongming Xie, Jianing Yu, Peng He

{"title":"GhCTEF2 encodes a PLS-type PPR protein required for chloroplast development and plastid RNA editing in cotton.","authors":"Huan He, Mengxue Cheng, Bowen Bao, Yanan Tian, Yating Zheng, Yuzhu Huo, Zengqiang Zhao, Zongming Xie, Jianing Yu, Peng He","doi":"10.1016/j.plantsci.2025.112478","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112478","url":null,"abstract":"Cotton is a significant cash crop and serves as a crucial raw material for the textile industry. The leaf, which is the site of photosynthesis in cotton plants, directly influences their growth and yield. Pentatricopeptide repeat (PPR) proteins are characterized by tandem 30-40 amino acid motifs. These proteins play a pivotal role in post-transcriptional regulation of organelle gene expression. In this study, we identified GhCTEF2 as a PLS-type PPR protein and determined its subcellular localization within chloroplasts, highlighting its essential involvement in chloroplast development. Virus-induced gene silencing assays revealed that knockdown of the GhCTEF2 gene resulted in macular phenotypes on cotton leaves and significantly reduced photosynthetic efficiency. Additionally, GhCTEF2-silenced plants exhibited incomplete chloroplasts with reduced thylakoids and grana structures. Furthermore, our findings showed that the downregulation of GhCTEF2 reduced the transcription levels of PEP-dependent genes and significantly decreased the content of the chloroplast LHCⅡ-T complex protein. Further studies showed that GhCTEF2 may interact with other editing factors to regulate the RNA editing process of ndhB, accD, and rps18. These findings offer valuable insights into future breeding strategies aimed at enhancing photosynthesis in cotton.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112478"},"PeriodicalIF":4.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide production and protein S-nitrosation in algae.

IF 4.2 2区生物学

Plant Science Pub Date : 2025-03-17 DOI: 10.1016/j.plantsci.2025.112472

Zoé Chaudron, Valérie Nicolas-Francès, Carole Pichereaux, Siham Hichami, Claire Rosnoblet, Angelique Besson-Bard, David Wendehenne

引用次数: 0

Analysis of the SlRAF-like B gene family in tomato and the molecular mechanism of SlRAF7 in regulating cold stress resistance.

IF 4.2 2区生物学

Plant Science Pub Date : 2025-03-15 DOI: 10.1016/j.plantsci.2025.112475

Junxiao Li, Qingpeng Li, Fan Wang, Ruoxi Ding, Yixuan Shang, Xiaohui Hu, Songshen Hu

{"title":"Analysis of the SlRAF-like B gene family in tomato and the molecular mechanism of SlRAF7 in regulating cold stress resistance.","authors":"Junxiao Li, Qingpeng Li, Fan Wang, Ruoxi Ding, Yixuan Shang, Xiaohui Hu, Songshen Hu","doi":"10.1016/j.plantsci.2025.112475","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112475","url":null,"abstract":"The SlRAF-like B gene family is crucial for the regulation of seed dormancy and response to osmotic stress. In this research, a bioinformatics approach was employed to identify a total of 18 members belonging to the SlRAF-like B gene family within the tomato genome. Phylogenetic analysis has categorized the identified SlRAF-like B genes into four distinct groups, revealing significant differences in conserved motifs and gene structure among the proteins within each cluster. Promoter sequence analysis revealed abundant stress, hormone, and light response elements, suggesting the involvement of SlRAF-like B genes in cold stress responses. RT-qPCR analysis showed that most SlRAF-like B genes are induced by cold stress. A knockout mutant of the SlRAF7 gene, belonging to the SlRAF-like B3 group, was generated and tested under normal and cold stress, demonstrating that SlRAF7 positively regulates cold resistance in tomato plants. Further analysis of antioxidant enzyme activities, expression of related genes, and key cold response genes (ICE1, CBFs, and COR genes) in different genotypes suggests that SlRAF7 may enhance cold resistance by modulating the antioxidant enzyme pathway and the CBF signaling pathway. This study provides initial insights into the physiological and molecular mechanisms that underlie cold stress tolerance in tomato, with a particular focus on the role of the SlRAF7 gene.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112475"},"PeriodicalIF":4.2,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of jasmonates in plant response to temperature stress.

IF 4.2 2区生物学

Plant Science Pub Date : 2025-03-15 DOI: 10.1016/j.plantsci.2025.112477

Aafia Iqbal, Henan Bao, Jian Wang, Huijie Liu, Jiangtao Liu, Liqun Huang, Dongping Li

引用次数: 0

Functional identification of mango MiEXPLA1a2 and MiEXPA4e1 genes in transgenic Arabidopsis and tomato 转基因拟南芥和番茄中芒果 MiEXPLA1a2 和 MiEXPA4e1 基因的功能鉴定。

IF 4.2 2区生物学

Plant Science Pub Date : 2025-03-13 DOI: 10.1016/j.plantsci.2025.112474

Xiang-juan Zhang, Ji-hong Yang, Jia-jun Li, Hui-jun Yang, Ming-qing Li, Yue-xing Zhang, Cong Luo, Xin-hua He

{"title":"Functional identification of mango MiEXPLA1a2 and MiEXPA4e1 genes in transgenic Arabidopsis and tomato","authors":"Xiang-juan Zhang, Ji-hong Yang, Jia-jun Li, Hui-jun Yang, Ming-qing Li, Yue-xing Zhang, Cong Luo, Xin-hua He","doi":"10.1016/j.plantsci.2025.112474","DOIUrl":"10.1016/j.plantsci.2025.112474","url":null,"abstract":"<div><div>Expansin (EXP) is an intrinsic regulator of plant cell expansion, and have been shown to play a role in each stages of plant growth and development. But has not yet been fully studied in mango. In this experiment, two pairs of homologous genes MiEXPA1s and MiEXPA4s were firstly excavated from mango genome. qRT-PCR analysis showed that the expression of MiEXPA1a2 was gradually increased with the development of mango fruits, while MiEXPA4e1 has the opposite expression pattern. In this study, the functions of two genes were explored by overexpression in Arabidopsis and tomato. MiEXPLA1a2 and MiEXPA4e1 genes with opposite expression levels showed similar gene functions. Compared with wild-type Arabidopsis (WT), overexpression of MiEXPA1a2 and MiEXPA4e1 Arabidopsis promoted early flowering, increased rosette leaves number, caused dwarf plants, and reduced the number of seeds. In addition, MiEXPA1a2 and MiEXPA4e1 transgenic plants significantly increased root length and survival rate under drought and salt stress treatments. It was also found that MiEXPA1a2 and MiEXPA4e1 promoted root length in response to gibberellin treatment, while ABA significantly inhibited it. We found similar phenotypes to Arabidopsis in transgenic tomato plants, such as promoted early flowering, reduced plant height, increased sepal length, affected the fruit and seed quality. Interestingly, MiEXPA4e1 is significantly shorter the pod length in Arabidopsis and reduced the fruit weight in tomato, while MiEXPA1a2 does not have this phenomenon. In conclusion, MiEXPLA1a2 and MiEXPA4e1 genes have potential applications in regulating plant flowering, regulating phenotype, and improving stress response.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112474"},"PeriodicalIF":4.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tomato DC1 domain protein SlCHP16 interacts with the 14–3-3 protein TFT12 to regulate flower development

IF 4.2 2区生物学

Plant Science Pub Date : 2025-03-13 DOI: 10.1016/j.plantsci.2025.112451

Guobin Li , Jiafa Wang , Licheng Xiao , Chunli Zhang , Dedi Zhang , Guo Ai , Minghua Yao , Changxing Li , Zonglie Hong , Zhibiao Ye , Junhong Zhang

{"title":"Tomato DC1 domain protein SlCHP16 interacts with the 14–3-3 protein TFT12 to regulate flower development","authors":"Guobin Li , Jiafa Wang , Licheng Xiao , Chunli Zhang , Dedi Zhang , Guo Ai , Minghua Yao , Changxing Li , Zonglie Hong , Zhibiao Ye , Junhong Zhang","doi":"10.1016/j.plantsci.2025.112451","DOIUrl":"10.1016/j.plantsci.2025.112451","url":null,"abstract":"<div><div>Flower development is of great significance for plant reproductive growth, but the molecular mechanisms underlying flower development remain to be fully understood. In this study, a tomato (Solanum lycopersicum L.) Divergent C1 (DC1) domain protein SlCHP16 was identified as a negative regulator of flower development. Overexpression of SlCHP16 led to the delay of flower bud development and failure of flowers to blossom and bear fruits. Conversely, down-regulation of SlCHP16 transcripts, via RNA interference (RNAi), led to formation of larger flowers in transgenic tomato plants. In SlCHP16-overexpressing plants, floral primordia and floral organs were initiated normally, but their subsequent growth and development were severely arrested. Transcriptome analysis showed that this arrest was associated with the changes in expression levels of a large number of genes involved in cell division and organ development. Tomato 14–3–3 protein 12 (TFT12) was identified as an interacting protein of SlCHP16 by tandem mass spectrometry, and its overexpression in tomato plants led to the formation of enlarged flowers. The presence of SlCHP16 disturbed the stability and homodimerization of TFT12 in plant cells. The results of this study demonstrate an inhibitory role of SlCHP16 in flower development in tomato by interaction with the 14–3–3 protein TFT12. This work provides new insights into the mechanisms that control development of floral organs.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112451"},"PeriodicalIF":4.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Industry perspective, genetics and genomics of peanut blanchability.

IF 4.2 2区生物学

Plant Science Pub Date : 2025-03-13 DOI: 10.1016/j.plantsci.2025.112473

Priya Shah, Graeme Wright, Chigozie V Nwosu, Daniel O'Connor, Peggy Tsatsos, Pasupuleti Janila, Kona Praveen, Kuldeep Singh, Sandip K Bera, Mahendar Thudi, Chittaranjan Kole, Rajeev K Varshney, Manish K Pandey

{"title":"Industry perspective, genetics and genomics of peanut blanchability.","authors":"Priya Shah, Graeme Wright, Chigozie V Nwosu, Daniel O'Connor, Peggy Tsatsos, Pasupuleti Janila, Kona Praveen, Kuldeep Singh, Sandip K Bera, Mahendar Thudi, Chittaranjan Kole, Rajeev K Varshney, Manish K Pandey","doi":"10.1016/j.plantsci.2025.112473","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112473","url":null,"abstract":"Blanching is the process of removing the testa or seed coat (skin) from peanuts, and a genotype's capacity to release its testa is referred to as its blanchability. The genotype, seed quality, harvest date, level of maturity, as well as the length of time and temperature of the post-harvest storage period, are said to have an impact on the peanut's blanchability. This characteristic is of enormous economic significance in the production of food items made from peanuts. Major research on this economically significant characteristic in breeding programmes has not, however, been done. Blanchability is a highly heritable and genetically regulated trait, thus breeding and selection should be effective. Blanchability reports to be fixed in the early generations due to its relatively simple genetic control, hence choice of parents which have good blanchability is of utmost importance in a breeding programme. As blanching percentage possess high genetic control with very low genotype × environment (G×E) interactions, effective selection for improved blanchability can be conducted in early generations. In peanut, it makes a great target for marker-assisted selection (MAS) but possess few factors that makes it difficult breeding target. These factors, include the high cost of labor to measure blanchability and the relatively large seed size in particular, prevent testing in early generations. In this review, we emphasize genetic research on this characteristic, its relationship to other traits, factors influencing it, methods of measurement, its industrial significance, as well as initiatives and difficulties related to its improvement.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112473"},"PeriodicalIF":4.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Casein kinase GhCKA1 positively regulates cotton resistance to Verticillium wilt 酪蛋白激酶 GhCKA1 积极调节棉花对轮纹病的抗性

IF 4.2 2区生物学

Plant Science Pub Date : 2025-03-12 DOI: 10.1016/j.plantsci.2025.112471

Luqi Chen , Lihong Zhao , Zili Feng , Feng Wei , Yalin Zhang , Heqin Zhu , Hongjie Feng , Jinglong Zhou

{"title":"Casein kinase GhCKA1 positively regulates cotton resistance to Verticillium wilt","authors":"Luqi Chen , Lihong Zhao , Zili Feng , Feng Wei , Yalin Zhang , Heqin Zhu , Hongjie Feng , Jinglong Zhou","doi":"10.1016/j.plantsci.2025.112471","DOIUrl":"10.1016/j.plantsci.2025.112471","url":null,"abstract":"<div><div>Verticillium wilt is an important disease that seriously affects the quality and yield of cotton. Fungal vascular diseases caused by Verticillium dahliae hinders the sustainable development of cotton cultivation. The most effective strategy for managing Verticillium wilt in cotton involves identifying resistance genes, investigating resistance mechanisms, and developing resistant varieties. In the laboratory, in our previous work, V. dahliae strain Vd080 was inoculated into both disease-resistant and disease-susceptible cotton strains, followed by a comprehensive transcriptomic analysis. The findings confirms the correlation between the gene GhCKA1 and disease resistance. In this study, silencing GhCKA1 expression led to reduced levels of reactive oxygen species, callose, and xylem accumulation, thereby inhibiting various defense responses and reducing cotton resistance to V. dahliae. Furthermore, we observed increased resistance to pathogens in Arabidopsis thaliana overexpressing GhCKA1. Subcellular localization experiments in tobacco indicated that GhCKA1 is localized within the nucleus. GUS staining analysis showed that the expression of the GhCKA1 promoter was influenced by pathogenic microorganisms. Additionally, we found that GhCKA1 interacts with aspartic proteases, an important proteolytic enzymes that play significant roles in metabolism and biological regulation. In conclusion, GhCKA1 enhances the resistance of cotton to V. dahliae and interacted with GhAsp1. Therefore, GhCKA1 may be a suitable molecular target to improve the resistance of cotton to Verticillium wilt, and provide a new breeding method for cotton to resist Werticillium wilt.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112471"},"PeriodicalIF":4.2,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of root hairs to soil compaction: A review

IF 4.2 2区生物学

Plant Science Pub Date : 2025-03-11 DOI: 10.1016/j.plantsci.2025.112461

Minwei Fu , Peng Xiong , Zhongbin Zhang , Xinhua Peng

{"title":"Responses of root hairs to soil compaction: A review","authors":"Minwei Fu , Peng Xiong , Zhongbin Zhang , Xinhua Peng","doi":"10.1016/j.plantsci.2025.112461","DOIUrl":"10.1016/j.plantsci.2025.112461","url":null,"abstract":"<div><div>In recent years, many studies have investigated the effects of soil compaction on plant root growth. However, root hairs, which are important parts of plants that anchor the soil and absorb nutrients and water, under compacted conditions have received limited attention. We reviewed the responses of root hair structure (behaviors), the rhizosheath, water and nutrient uptake by root hairs, plant hormones and crop species associated with root hairs to soil compaction and proposed potential solutions to mitigate the impacts of soil compaction on root hairs. Soil compaction generally reduces root hair length and density; however, a few studies have reported opposite results for reasons that are unclear. Root hairs exhibit limited water and nutrient uptake capacity, whereas high levels of ethylene have been observed in response to soil compaction. The scales of the effects described above are closely related to genotype. Bio-tillage, the application of ethylene inhibitors, the use of microorganisms and the breeding of soil compaction-tolerant crops may be effective methods to promote root hair growth under soil compaction. High-resolution computed tomography (CT) techniques are needed in the future to study root hair interactions with non-homogeneous soils over large scales.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"355 ","pages":"Article 112461"},"PeriodicalIF":4.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0