Plant Science最新文献

Review: Trehalose and its role in plant adaptation to salinity stress 综述：海藻糖及其在植物适应盐胁迫中的作用

IF 4.2 2区生物学

Plant Science Pub Date : 2025-04-29 DOI: 10.1016/j.plantsci.2025.112533

Ehab A. Ibrahim

{"title":"Review: Trehalose and its role in plant adaptation to salinity stress","authors":"Ehab A. Ibrahim","doi":"10.1016/j.plantsci.2025.112533","DOIUrl":"10.1016/j.plantsci.2025.112533","url":null,"abstract":"<div><div>Salinity stress is an important abiotic stress that negatively affects plant growth and yield as it causes oxidative damage, osmotic stress, and ionic balance disturbances. To overcome these problems, the naturally occurring disaccharide trehalose has received increasing attention due to its multiple roles in functions essential in enhancing plant tolerance to salt. This review examines the current information on how trehalose enhances salinity tolerance, highlighting its biochemical, physiological, and signaling functions. Trehalose scavenges reactive oxygen species and activates important antioxidant enzymes to stabilize cellular structures, maintain osmotic equilibrium, and reduce oxidative damage. Furthermore, it boosts photosynthetic efficiency by maintaining chloroplast integrity and stabilizing photosystems and metabolic enzymes under saline conditions. As climate change increases the severity of salt stress, incorporating trehalose into crop management practices has promising potential to advance sustainable agriculture and ensure global food security. Despite significant progress, the specific mechanisms of trehalose's action, especially its role in signaling pathways and its interactions with other metabolites, remain active research areas. This review explores the potential applications of trehalose in sustainable agriculture while providing a foundation for further research into its mechanisms in regulating plant growth, development, and stress resistance.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112533"},"PeriodicalIF":4.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-mediated gene editing of MsCLE3a confers compact alfalfa architecture CRISPR/ cas9介导的MsCLE3a基因编辑赋予了紧凑的苜蓿结构

IF 4.2 2区生物学

Plant Science Pub Date : 2025-04-29 DOI: 10.1016/j.plantsci.2025.112532

Hefan Li , Cheng Li , García-Caparrós Pedro , Qian Zhang , Hua Jiang , Yongping Yang , Yuanwen Duan , Xudong Sun

{"title":"CRISPR/Cas9-mediated gene editing of MsCLE3a confers compact alfalfa architecture","authors":"Hefan Li , Cheng Li , García-Caparrós Pedro , Qian Zhang , Hua Jiang , Yongping Yang , Yuanwen Duan , Xudong Sun","doi":"10.1016/j.plantsci.2025.112532","DOIUrl":"10.1016/j.plantsci.2025.112532","url":null,"abstract":"<div><div>Alfalfa (<em>Medicago sativa</em> L.) is a globally major forage crop for livestock due to its superior nutritional value and palatability. As a premium forage species, alfalfa breeding has traditionally emphasized high yield. In this study, we designed guide RNA (gRNA) to target the coding sequence of the <em>MsCLE3a</em> gene, and constructed a CRISPR/Cas9 vector to generate knockouts. Five mutant lines were successfully isolated, displaying upright petioles and a compact ternately compound leaf phenotype during the seedling stage. Cryo-scanning electron microscopy (Cryo-SEM) was employed to analyze cell morphology on the abaxial leaf surface. The results indicated that the cells on the abaxial side of the <em>Mscle3a</em> mutants were significantly elongated compared to the wild type (WT). Transcriptomic analysis further uncovered the down-regulation of brassinosteroid (BR) biosynthesis genes in <em>Mscle3a</em> mutants, which might underlie the mechanisms regulating petiole angle. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed a major reduction in the transcript levels of several key genes involved in BR signaling pathways in the <em>Mscle3a</em> mutants relative to WT. This transcriptional down-regulation was strongly associated with the observed alterations in leaf petiole angle (LPA) in the mutant lines. Our findings provide novel insights into the novel regulatory role of <em>MsCLE3a</em> in alfalfa development and pinpoint potential targets for manipulating LPA in crop breeding programs.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112532"},"PeriodicalIF":4.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NMD-mediated posttranscriptional regulation fine-tunes the NLR-WRKY regulatory module to modulate bacterial defense response nmd介导的转录后调控微调NLR-WRKY调控模块来调节细菌防御反应

IF 4.2 2区生物学

Plant Science Pub Date : 2025-04-26 DOI: 10.1016/j.plantsci.2025.112528

Zeeshan Nasim , Nouroz Karim , Ikram Blilou , Ji Hoon Ahn

{"title":"NMD-mediated posttranscriptional regulation fine-tunes the NLR-WRKY regulatory module to modulate bacterial defense response","authors":"Zeeshan Nasim , Nouroz Karim , Ikram Blilou , Ji Hoon Ahn","doi":"10.1016/j.plantsci.2025.112528","DOIUrl":"10.1016/j.plantsci.2025.112528","url":null,"abstract":"<div><div>Nonsense-mediated mRNA decay (NMD) is a conserved eukaryotic surveillance system that maintains transcriptome integrity by degrading aberrant RNA transcripts. NMD ensures proper growth and development by preventing autoimmunity through the direct regulation of nucleotide-binding, leucine-rich repeat (<em>NLR</em>) genes. Whether NMD directly regulates <em>WRKY</em> genes remains unclear, despite their upregulation in NMD-deficient plants, and potential feedback between <em>NLR</em>s and <em>WRKY</em>s is also poorly understood. In this study, we showed that NMD also directly regulates a subset of <em>WRKY</em> (<em>WRKY15</em>, <em>18</em>, <em>25</em>, <em>33</em>, <em>46</em>, <em>60</em>, and <em>70</em>) genes, particularly at lower temperatures (16°C). NMD signature-containing transcripts of <em>WRKY46</em> and <em>WRKY70</em>, selected as representative NMD-regulated <em>WRKY</em> genes, showed increased half-lives in NMD-deficient mutants. Transcriptome analyses showed that these seven NMD-regulated <em>WRKY</em> genes are induced in response to bacterial infection. Potential homologues of these seven NMD-regulated <em>WRKY</em> genes in maize and rice showed similar induction in response to bacterial pathogen infection. Furthermore, these NMD-regulated <em>WRKY</em> genes are induced in plants overexpressing <em>RESISTANT TO P. SYRINGAE 4</em> (<em>RPS4</em>) in a temperature-dependent manner. By using ChIP-seq and DAP-seq data of WRKY transcription factors, we showed that WRKYs potentially regulate a significant number of <em>NLR</em> genes by directly binding to the W-box in their promoter regions. Taken together, our findings revealed that in addition to the <em>NLR</em>s, the NMD machinery also regulates <em>WRKY</em> genes to keep the basal defense levels in check and the WRKY transcription factors directly regulate <em>NLR</em> genes to constitutes a positive feedback regulatory loop to optimize the plant response to invading pathogens.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112528"},"PeriodicalIF":4.2,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA modification: A contemporary review of pseudouridine (Ψ) and its role in functional plant biology RNA修饰：伪尿嘧啶（Ψ）及其在功能性植物生物学中的作用的当代综述

IF 4.2 2区生物学

Plant Science Pub Date : 2025-04-24 DOI: 10.1016/j.plantsci.2025.112522

Ahsan Khan , Gatera Anicet , Hafiz Umair Asdullah , Muhammad Ahmad Hassan , Youhong Song

引用次数: 0

NRDN: A novel nuclear degradation tag for targeted protein regulation in Arabidopsis NRDN：一种用于拟南芥靶向蛋白调控的新型核降解标签

IF 4.2 2区生物学

Plant Science Pub Date : 2025-04-24 DOI: 10.1016/j.plantsci.2025.112529

Liting Shen , Huizhen Huang , Daqi Yan , Yongsheng Ye , Jun Hu

引用次数: 0

Calcium-mediated mitigation strategies and novel approaches to alleviate arsenic induced plant stress 钙介导的缓解策略和缓解砷诱导植物胁迫的新途径

IF 4.2 2区生物学

Plant Science Pub Date : 2025-04-24 DOI: 10.1016/j.plantsci.2025.112527

Mohammad Faizan , Pravej Alam , Sumera Iqbal , Zainab Waheed , Abdullah Eren , Anas Shamsi , Moyad Shahwan

{"title":"Calcium-mediated mitigation strategies and novel approaches to alleviate arsenic induced plant stress","authors":"Mohammad Faizan , Pravej Alam , Sumera Iqbal , Zainab Waheed , Abdullah Eren , Anas Shamsi , Moyad Shahwan","doi":"10.1016/j.plantsci.2025.112527","DOIUrl":"10.1016/j.plantsci.2025.112527","url":null,"abstract":"<div><div>One worldwide environmental concern is the presence of potentially hazardous elements (PTEs) in air, soil, and water resources. Arsenic is one of the PTEs that is thought to be the most poisonous and carcinogenic. Plants exposed to arsenic may experience several morphological, physiological, and biochemical changes-even at extremely low concentrations. Arsenic toxicity to plants varies with its speciation in plants (e.g., arsenite, As(III); arsenate, As(V)), with the kind of plant species, and with other soil parameters affecting arsenic accumulation in plants, according to new study on arsenic in the soil-plant system. Arsenic stress modifies metabolic cascades in plants at different developmental stages by affecting the pattern of gene expressions mediated by small non-coding RNAs (micro-RNAs), which are essential for plant adaptation to oxidative stress and play a key role in the moderation of numerous cellular processes. In this review, we investigated the impact of calcium (Ca<sup>2 +</sup>) on the toxicity of arsenic in plant and soil environments. Plant grown with arsenic exhibited enhanced arsenic uptake, increased oxidative stress and growth inhibition. Arsenic toxicity modulates carbohydrate, lipid, and protein metabolism along with DNA structure. Role of Ca<sup>2+</sup>, Ca channels and Ca sensors to signaling pathways also described briefly. A worldwide issue for humanity is the poisoning of soil ecosystems by arsenic. Its toxicity, tolerance, and phytoremediation of polluted soils utilizing calcium were the main points of the recent review, which also highlighted the significant mechanisms of arsenic in soil-plant systems.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112527"},"PeriodicalIF":4.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycosyltransferases: Pioneering roles in agriculture and medicine 糖基转移酶：在农业和医学中的先锋作用

IF 4.2 2区生物学

Plant Science Pub Date : 2025-04-23 DOI: 10.1016/j.plantsci.2025.112520

Qian Liu , Fabin Yang , Yanan Zhang , Qingli Liu , Wenjian Ma , Ying Wang

{"title":"Glycosyltransferases: Pioneering roles in agriculture and medicine","authors":"Qian Liu , Fabin Yang , Yanan Zhang , Qingli Liu , Wenjian Ma , Ying Wang","doi":"10.1016/j.plantsci.2025.112520","DOIUrl":"10.1016/j.plantsci.2025.112520","url":null,"abstract":"<div><div>Glycosyltransferases (GTs) belong to a diverse family of enzymes that catalyze the transfer of sugar moieties from activated donor sugars to specific acceptors, thus playing a crucial roles in various biological processes. This review explores the pioneering roles of uridine diphosphate-dependent GTs (UGTs), which use uridine diphosphate glucose as donors. UGTs have also been extensively studied in agricultural and medical fields, emphasizing their potential to revolutionize these sectors. In the agricultural sector, the genetic engineering of UGTs has demonstrate potential in developing crops with enhanced stress tolerance, regulated plant development, and increased resistance to pests and diseases. These advancements not only contribute to sustainable farming practices but also address global food security challenges by facilitating the production of more resilient plant varieties. Furthermore, UGTs facilitate the synthesis of complex carbohydrates and glycoconjugates in plants, which are critical for developing drugs and therapeutic strategies targeting various ailments, including cancer and infectious diseases. Thus, this review explored the functions and synthesis methods of flavonoid glycosides, terpenoid glycosides, and polyketosides in detail. Moreover, owing to the functional diversity of UGTs, numerous research methods were reviewed, and novel, more valuable UGTs will be obtained. In summary, this study synthesizes the current research findings and discusses future perspectives to underscore the transgenic technology and synthetic biological impact of UGTs on agriculture and medicine and bridge the gap between fundamental science and practical applications.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112520"},"PeriodicalIF":4.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root hair: An important guest-meeting avenue for rhizobia in legume–Rhizobium symbiosis 根毛：豆科植物-根瘤菌共生中根瘤菌的重要会客途径

IF 4.2 2区生物学

Plant Science Pub Date : 2025-04-22 DOI: 10.1016/j.plantsci.2025.112518

Jingwen Xiao , Wenxu Liu , Bicong Wu , Yuling Zhang , Sha Li , En Li

引用次数: 0

Simple and semi-high throughput determination of total phenolic, anthocyanin, flavonoid content, and total antioxidant capacity of model and crop plants for cell physiological phenotyping 简单、半高通量测定模型和作物植物的总酚、花青素、类黄酮含量和总抗氧化能力，用于细胞生理表型分析

IF 4.2 2区生物学

Plant Science Pub Date : 2025-04-22 DOI: 10.1016/j.plantsci.2025.112524

Daniel Buchvaldt Amby , Mengistu Fentahun Mekureyaw , Saqib Saleem Akhtar , Chandana Pandey , Thomas Roitsch

{"title":"Simple and semi-high throughput determination of total phenolic, anthocyanin, flavonoid content, and total antioxidant capacity of model and crop plants for cell physiological phenotyping","authors":"Daniel Buchvaldt Amby , Mengistu Fentahun Mekureyaw , Saqib Saleem Akhtar , Chandana Pandey , Thomas Roitsch","doi":"10.1016/j.plantsci.2025.112524","DOIUrl":"10.1016/j.plantsci.2025.112524","url":null,"abstract":"<div><div>Plants biosynthesize a wide range of antioxidants capable of attenuating ROS-induced oxidative damage. There exist several in vitro methods to analyze antioxidants and total antioxidant capacity from different tissues and of various plant species. We have established a single, fast and cost-efficient extraction protocol combined with a semihigh throughput 96-well plate assay methods for determination of the level of the key antioxidants phenolics, anthocyanins and flavonoids in combination with the determination of total antioxidant capacity using ferric reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC). The method was optimized and verified with samples from different strawberry species and cultivars with known differences in the parameters measured. This method proved to be suitable for analyses of eight model and crop plants, and distinct antioxidant signatures were determined for the different tissues and organs analyzed, including leaf, root, fruit, spike, and tuber samples. The method was robust and was shown in two case studies to be a resource-efficient and fast experimental platform also to assess biotic and abiotic stress responses, notably including fungal infection and the impact of a progressive drought regime. Since method was adapted for a semi-high throughput 96-well assay format it is well-suited for integration of cell physiological phenotyping into a holistic phenomics approach for germplasm assessment and plant breeding screening. This analytical platform uses microplate spectrophotometer which proved to be suitable to determine the antioxidant contents and total antioxidant capacity signatures of various plant species and tissues with similar findings as reported in literature.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112524"},"PeriodicalIF":4.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of the lectin receptor-like kinase gene OsLecRK-S.7 inhibits plant growth and enhances disease resistance in rice 凝集素受体样激酶基因OsLecRK-S的过表达。7抑制植物生长，增强水稻抗病性

IF 4.2 2区生物学

Plant Science Pub Date : 2025-04-22 DOI: 10.1016/j.plantsci.2025.112517

Xiaoqun Peng , Yilin Li , Jingmei Xu , Ying Zeng , Kun Li , Xiangyi Guo , Zikang Zhang , Xiaoyan Tang , Menglong Wang

{"title":"Overexpression of the lectin receptor-like kinase gene OsLecRK-S.7 inhibits plant growth and enhances disease resistance in rice","authors":"Xiaoqun Peng , Yilin Li , Jingmei Xu , Ying Zeng , Kun Li , Xiangyi Guo , Zikang Zhang , Xiaoyan Tang , Menglong Wang","doi":"10.1016/j.plantsci.2025.112517","DOIUrl":"10.1016/j.plantsci.2025.112517","url":null,"abstract":"<div><div>Lectin receptor-like kinases (LecRKs) are a critical class of plant proteins that play essential roles in plant development as well as in responses to both biotic and abiotic stresses. In this study, we found that overexpression of the <span>L</span>-type Lectin receptor kinase gene <em>OsLecRK-S.7</em> severely inhibits plant growth and triggers spontaneous cell death. Meanwhile, immune responses, including pathogenesis-related (PR) gene expression and reactive oxygen species (ROS) accumulation, were elevated in <em>OsLecRK-S.7</em> overexpressing plants. Kinase inactivation experiments demonstrated that kinase activity was essential for OsLecRK-S.7-mediated constitutive immunity. Infection assays further demonstrated that overexpression of <em>OsLecRK-S.7</em> enhances rice resistance to bacterial blight. Additionally, bimolecular fluorescence complementation (BiFC) and pull-down experiments identified interactions between OsLecRK-S.7 and receptor-like cytoplasmic kinases (RLCKs) OsRLCK118, OsRLCK185, and OsRLCK107 that are involved in immune signaling. These findings suggest that OsLecRK-S.7 is a significant regulator of plant immunity, likely promoting cell death and immune responses through its interactions with OsRLCK118, OsRLCK185, and OsRLCK107.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112517"},"PeriodicalIF":4.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0