Plant Science最新文献

SlTDF1: A Key Regulator of Tapetum Degradation and Pollen Development in Tomato. SlTDF1：番茄被膜降解和花粉发育的关键调控因子

IF 4.2 2区生物学

Plant Science Pub Date : 2024-11-14 DOI: 10.1016/j.plantsci.2024.112321

Zhengliang Sun, Baohui Cheng, Yanhong Zhang, Liangzhe Meng, Yuhe Yao, Yan Liang

引用次数: 0

Intragenic cytosine methylation modification regulates the response of SUCLα1 to lower temperature in Solanaceae 基因内胞嘧啶甲基化修饰调节茄科植物 SUCLα1 对较低温度的响应。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-11-13 DOI: 10.1016/j.plantsci.2024.112320

Cuihua Xin , Junjie Wang , Junling Chi , Yang Xu , Ruiping Liang , Lei Jian , Liangming Wang , Jiangbo Guo

{"title":"Intragenic cytosine methylation modification regulates the response of SUCLα1 to lower temperature in Solanaceae","authors":"Cuihua Xin , Junjie Wang , Junling Chi , Yang Xu , Ruiping Liang , Lei Jian , Liangming Wang , Jiangbo Guo","doi":"10.1016/j.plantsci.2024.112320","DOIUrl":"10.1016/j.plantsci.2024.112320","url":null,"abstract":"<div><div>The tricarboxylic acid cycle (TCAC) is a fundamental metabolic process governing matter and energy in plant cells, playing an indispensable role. However, its involvement in responding to low temperature stress in potato remains poorly understood. Previous studies have identified succinyl-CoA ligase (SUCL), which catalyzes the phosphorylation of TCAC substrates, as a gene associated with lower temperatures. Nevertheless, its function in potato's response to lower temperatures remains unclear. Phylogenetic analysis has revealed that Solanum tuberosum possesses α and β subunits of SUCL, which cluster with those of Solanum lycopersicum, Nicotiana tabacum and Nicotiana benthamiana. Further investigation has shown that StSUCLα1 is predominantly located within mitochondria. Low temperatures induce methylation modification alterations at 11 intragenic cytosine sites and lead to changes in StSUCLα1 expression levels. Correlation analysis suggests that alterations in intragenic cytosine methylation sites of SUCLα1 may be associated with MET1. Knocking down NbSUCLα1, the homologous gene of StSUCLα1 in N. benthamiana, results in increased susceptibility to low temperature stress in plants. In summary, we have confirmed that SUCLα1 is a key gene modulated by intragenic cytosine methylation in response to lower temperatures, providing a novel target for genetic breeding aimed at enhancing potato tolerance to low temperature stress.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"350 ","pages":"Article 112320"},"PeriodicalIF":4.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The PHD transcription factor ThPHD5 regulates antioxidant enzyme activity to increase salt tolerance in Tamarix hispida. PHD 转录因子 ThPHD5 可调节抗氧化酶的活性，从而提高柽柳的耐盐性。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-11-13 DOI: 10.1016/j.plantsci.2024.112319

Yao-Shuo Tan, Jing-Hang Li, Pei-Long Wang, Dan-Ni Wang, Bai-Chao Liu, Sonethavy Phetmany, Yong-Xi Li, Qing-Jun Xie, Cai-Qiu Gao

{"title":"The PHD transcription factor ThPHD5 regulates antioxidant enzyme activity to increase salt tolerance in Tamarix hispida.","authors":"Yao-Shuo Tan, Jing-Hang Li, Pei-Long Wang, Dan-Ni Wang, Bai-Chao Liu, Sonethavy Phetmany, Yong-Xi Li, Qing-Jun Xie, Cai-Qiu Gao","doi":"10.1016/j.plantsci.2024.112319","DOIUrl":"https://doi.org/10.1016/j.plantsci.2024.112319","url":null,"abstract":"PHD proteins are an important class of transcription factors (TFs) that are widely distributed in eukaryotes and play crucial roles in many aspects of plant growth, development and response to stress. We identified a transcription factor, ThPHD5, from the PHD family in Tamarix hispida based on its potential involvement in abiotic stress response processes. In this study, the salt tolerance function of ThPHD5 from T. hispida was further characterized. The qRTPCR results showed ThPHD5 was expressed in response to NaCl, PEG and ABA treatments. Transient transformation analysis revealed that ThPHD5 improved salt tolerance in T. hispida by increasing POD and SOD activity and decreasing the MDA, total ROS content and electrolyte leakage. To explore the salt tolerance mechanism of the ThPHD5 TF, its binding DNA motifs and potential downstream regulatory genes were analyzed. The results showed that ThPHD5 affect the expression of 7 antioxidant enzyme-related genes. The Yeast one-hybrid (Y1H) and Electrophoretic Mobility Shift Assay (EMSA) results indicated ThPHD5 could bind to ABRE, MYB and Dof cis-acting elements. ChIP-PCR further confirmed ThPHD5 exercise its regulatory function by directly binding motifs on the ThPOD16, ThSOD and ThSOD1 promoters. Taken together, these findings indicate the ThPHD5 TF improves salt tolerance in T. hispida by regulating the expression of antioxidant enzyme-related genes to increase antioxidant enzyme activity, enhance the ROS scavenge ability, reduce ROS accumulation and cellular damage.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112319"},"PeriodicalIF":4.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analyses at specific plastochrons reveal timing and involvement of phytosulfokine in maize vegetative phase change 特定质点的转录组分析揭示了植物生长素参与玉米植被相变的时间和作用。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-11-12 DOI: 10.1016/j.plantsci.2024.112317

Krista Osadchuk, Ben Beydler, Chi-Lien Cheng, Erin Irish

引用次数: 0

Tree biology: From genomics to genetic improvement 编辑树木生物学：从基因组学到基因改良。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-11-10 DOI: 10.1016/j.plantsci.2024.112315

Jinbo Shen, Wan-Feng Li, Chang Liu, Shuai Zheng

引用次数: 0

Functional identification of two Glycerol-3-phosphate Acyltransferase5 homologs from Chenopodium quinoa 藜麦中两种甘油-3-磷酸酰基转移酶5同源物的功能鉴定

IF 4.2 2区生物学

Plant Science Pub Date : 2024-11-07 DOI: 10.1016/j.plantsci.2024.112313

Zhen Wang , Yuxin Liu , Haodong Huang , Zhifu Zheng , Shiyou Lü , Xianpeng Yang , Changle Ma

{"title":"Functional identification of two Glycerol-3-phosphate Acyltransferase5 homologs from Chenopodium quinoa","authors":"Zhen Wang , Yuxin Liu , Haodong Huang , Zhifu Zheng , Shiyou Lü , Xianpeng Yang , Changle Ma","doi":"10.1016/j.plantsci.2024.112313","DOIUrl":"10.1016/j.plantsci.2024.112313","url":null,"abstract":"<div><div>Glycerol-3-phosphate acyltransferase5 (GPAT5) is the key enzyme in suberin biosynthesis in Arabidopsis, tomato and Sarracenia purpurea. However, little is known about whether GPAT5 function is conserved in halophytes. In this study, we identified two GPAT5 homologs, CqGPAT5a and CqGPAT5b, in Chenopodium quinoa, the typical halophyte. Using RT-qPCR, we found that CqGPAT5a and CqGPAT5b were highly expressed in quinoa roots and rapidly induced by high salt stress. CqGPAT5a and CqGPAT5b were localized to the endoplasmic reticulum and found to have glycerol-3-phosphate acyltransferase activity using yeast complementation assays. Compared with CqGPAT5b, CqGPAT5a showed relatively weaker function and less protein abundance when expressed in yeast, Arabidopsis or Nicotiana benthamiana. Subsequently, we identified a serine (S) to leucine (L) variation in the CqGPAT5a protein sequence (S251L) compared with CqGPAT5b, located in the connecting region between the second and third transmembrane domains. Site-directed mutagenesis together with yeast mutant complementation and transient expression in tobacco demonstrated that this variation significantly affected CqGPAT5a activity and protein abundance. These findings expand our understanding of GPAT5 and provide new evidence that GPAT5 may be functionally conserved in halophytes.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"350 ","pages":"Article 112313"},"PeriodicalIF":4.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Duplicate MADS-box genes with split roles and a genetic regulatory network of floral development in long-homostyle common buckwheat 具有不同作用的重复 MADS-box 基因和长同源普通荞麦花发育的遗传调控网络。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-11-05 DOI: 10.1016/j.plantsci.2024.112316

Xinyu Jiao , Yamin Li , Qingyu Yang, Xiangjian Chen, Lan Luo, Yuzhen Liu, Zhixiong Liu

{"title":"Duplicate MADS-box genes with split roles and a genetic regulatory network of floral development in long-homostyle common buckwheat","authors":"Xinyu Jiao , Yamin Li , Qingyu Yang, Xiangjian Chen, Lan Luo, Yuzhen Liu, Zhixiong Liu","doi":"10.1016/j.plantsci.2024.112316","DOIUrl":"10.1016/j.plantsci.2024.112316","url":null,"abstract":"<div><div>The classic ABC model postulates how three classes of floral homeotic genes (A, B and C) work in a combinational way to confer organ identity to each whorl that make up a perfect flower in core eudicot plants. Fagopyrum esculentum (Polygonaceae) produces dimorphic flowers with single whorl showy tepals, representing a considerable difference with most core eudicots flowers. Here, we explain in detail the function of a duplicated pair of floral homeotic genes involved in the formation of tepals and stamens in the LH F. esculentum. FaesAP1_1 and FaesAP1_2 work together to specify tepal identity. FaesAP3_1/2 or FaesPI_1/2 have redundant function in specifying filament identity, while FaesAP3_2 and FaesPI_2 also retain a conserved role in specifying anther development and gain novel function in style length determination. However, FaesPI_1 gain novel function in floral color formation. In addition, FaesAG can directly regulate stamen and pistil development or binds to the CArG-box of pFaesPI_1 to indirectly regulate stamen and pistil development by a gene regulatory pathway involving FaesAP1_1/2, FaesAP3_1/2 and FaesPI_1/2. Moreover, FaesAP1_1/2 can directly or indirectly regulate B-class gene (FaesAP3_1/2 and FaesPI_1/2) expression to be involved in floral development. Our work has led to detailed insights into the MADS-box gene regulatory networks that control floral developmental process in LH F. esculentum.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"350 ","pages":"Article 112316"},"PeriodicalIF":4.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional analysis of (E)-β-farnesene synthases involved in accumulation of (E)-β-farnesene in German chamomile (Matricaria chamomilla L.) 参与德国洋甘菊（Matricaria chamomilla L.）中(E)-β-法呢烯积累的(E)-β-法呢烯合成酶的功能分析。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-11-03 DOI: 10.1016/j.plantsci.2024.112314

Yuling Tai , Haiyan Wu , Lu Yang , Yi Yuan, Youhui Chen, Honggang Wang, Yifan Jin, Luyao Yu, Shuangshuang Li, Feng Shi

{"title":"Functional analysis of (E)-β-farnesene synthases involved in accumulation of (E)-β-farnesene in German chamomile (Matricaria chamomilla L.)","authors":"Yuling Tai , Haiyan Wu , Lu Yang , Yi Yuan, Youhui Chen, Honggang Wang, Yifan Jin, Luyao Yu, Shuangshuang Li, Feng Shi","doi":"10.1016/j.plantsci.2024.112314","DOIUrl":"10.1016/j.plantsci.2024.112314","url":null,"abstract":"<div><div>German chamomile (Matricaria chamomilla L.) is a traditional medicinal aromatic plant, and the sesquiterpenoids in its flowers have important medicinal value. The (E)-β-farnesene (EβF) is one of the active sesquiterpenoid components and is also a major component of aphid alarm pheromones. In this study, two EβF synthase (βFS) genes (McβFS1 and McβFS2), were cloned from German chamomile. Subcellular localization analysis showed that both McβFS1 and McβFS2 were localized in the cytoplasm and nucleus. Tissue-specific expression analysis revealed that McβFS1 and McβFS2 were expressed in all flower stages, with the highest levels observed during the tubular flower extension stage. Prokaryotic expression and enzyme activity results showed that McβFS1 and McβFS2 possess catalytic activity. Overexpression of McβFS1 and McβFS2 in the hairy roots of German chamomile led to the accumulation of EβF, demonstrating enzyme activity in vivo. The promoters of McβFS1 and McβFS2 were cloned and analyzed. After treating German chamomile with methyl jasmonate (MeJA) and methyl salicylate (MeSA), the transcription levels of McβFS1 and McβFS2 were found to be regulated by both hormones. In addition, feeding experiments showed that aphid infestation upregulated the expression levels of McβFS1 and McβFS2. Our study provides valuable insights into the biosynthesis of EβF, laying a foundation for further research into its metabolic pathways.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"350 ","pages":"Article 112314"},"PeriodicalIF":4.2,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutation of rice SM1 enhances solid leaf midrib formation and increases methane emissions 水稻 SM1 基因突变会促进固体叶中肋的形成并增加甲烷排放。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-29 DOI: 10.1016/j.plantsci.2024.112312

Hongrui Jiang , Weimin Cheng , Chunpeng Chen , Cheng Fang , Yue Zhan , Liangzhi Tao , Yang Yang , Xianzhong Huang , Kun Wu , Xiangdong Fu , Yuejin Wu , Binmei Liu , Yafeng Ye

{"title":"Mutation of rice SM1 enhances solid leaf midrib formation and increases methane emissions","authors":"Hongrui Jiang , Weimin Cheng , Chunpeng Chen , Cheng Fang , Yue Zhan , Liangzhi Tao , Yang Yang , Xianzhong Huang , Kun Wu , Xiangdong Fu , Yuejin Wu , Binmei Liu , Yafeng Ye","doi":"10.1016/j.plantsci.2024.112312","DOIUrl":"10.1016/j.plantsci.2024.112312","url":null,"abstract":"<div><div>The leaf midrib system is essential for plant growth and development, facilitating nutrient transport, providing structural support, enabling gas exchange, and enhancing resilience to environmental stresses. However, the molecular mechanism regulating leaf midrib development is still unclear.In this study, we reported a rice solid midrib 1 (sm1) mutant, exhibiting solid leaf aerenchyma and abaxial rolling leaves due to abnormal development of parenchyma and bulliform cells. Map-based cloning revealed that SM1 encodes a litter zipper protein (ZPR). SM1 was mainly expressed in the sheaths and basal midrib and was associated with the nucleus. Further experiments indicated that SM1 can interact with OSHB1, preventing the formation of OSHB:OSHB dimers and subsequently repressing the expression of OSH1 involved in the regulation and maintenance of apical stem meristem formation. The sm1 mutant reduced long-distance oxygen transport ability from shoot to root. The impaired oxygen transport in the sm1 mutant may have contributed to the increase in methanogens and elevated methane emissions. Collectively, our findings revealed that the SM1-OSHB1-OSH1 modules regulate leaf aerenchyma development in rice. These modules not only enhance our understanding of the molecular mechanism of rice leaf aerenchyma development but also offer insights for reducing methane emissions through genetic modification.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"350 ","pages":"Article 112312"},"PeriodicalIF":4.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the fructose 1,6-bisphosphate aldolase (FBA) family genes in maize and analysis of the phosphorylation regulation of ZmFBA8 鉴定玉米中的 1,6-二磷酸果糖醛缩酶（FBA）家族基因并分析 ZmFBA8 的磷酸化调控。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-29 DOI: 10.1016/j.plantsci.2024.112311

Zijuan Zhang , Xiaoman Li , Yiying Zhang , Jiajia Zhou , Yanmei Chen , Yuan Li , Dongtao Ren

引用次数: 0