Plant Science最新文献

Genomics of sterols biosynthesis in plants: Current status and future prospects

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-15 DOI: 10.1016/j.plantsci.2025.112426

Harshad A. Shirke , Ashwini M. Darshetkar , Vikas B. Naikawadi , P.B. Kavi Kishor , Tukaram D. Nikam , Vitthal T. Barvkar

{"title":"Genomics of sterols biosynthesis in plants: Current status and future prospects","authors":"Harshad A. Shirke , Ashwini M. Darshetkar , Vikas B. Naikawadi , P.B. Kavi Kishor , Tukaram D. Nikam , Vitthal T. Barvkar","doi":"10.1016/j.plantsci.2025.112426","DOIUrl":"10.1016/j.plantsci.2025.112426","url":null,"abstract":"<div><div>Sterols produced by bacteria and all eukaryotic organisms are essential for membrane functionality and stability. They play a vital role in growth, development and in abiotic stress tolerance. They are involved in diverse responses to biotic and abiotic stresses that lead to providing resistance against multiple diseases. Additionally, sterols serve as defensive compounds against herbivorous insects and animals. Phytosterols derived from plants, improve human nutrition and health and cure different ailments. The biosynthetic pathways for sterols and triterpenes exhibit similarities until the synthesis of 2,3-oxidosqualene. The complexity of sterol pathways increases during the advanced stages of polycyclic structure synthesis, and remain poorly comprehended in plants. This review explores the various omics techniques used to unveil the functions of genes associated with the phytosterol pathways. The study investigates the biosynthetic gene clusters to clarify the structural arrangements of genes linked to metabolic pathways. Both the upstream and downstream genes associated with these pathways, as well as their evolutionary connections and interrelations within the pathways were brought to the forefront. Moreover, developing strategies to unravel the biosynthesis completely and their multi-layered regulation are crucial to comprehend the global roles that sterols play in plant growth, development, stress tolerance and in imparting defence against pathogens.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112426"},"PeriodicalIF":4.2,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Molecular Regulation on Plant Oil Synthesis.

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-11 DOI: 10.1016/j.plantsci.2025.112428

Hansheng Zhang, Tinghui Feng, Qinxiang Chang

{"title":"Impact of Molecular Regulation on Plant Oil Synthesis.","authors":"Hansheng Zhang, Tinghui Feng, Qinxiang Chang","doi":"10.1016/j.plantsci.2025.112428","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112428","url":null,"abstract":"<p><p>The synthesis of lipids in plants is essential for their growth and development, and it has wide-ranging applications in various fields, including diet and industry. In the majority of plants, the principal unsaturated fatty acids (UFAs) are three C18 varieties: oleic acid (18:1), linoleic acid (18:2), and α-linolenic acid (18:3). Despite the clear delineation of the principal biosynthetic pathways of fatty acids in plants, numerous unresolved issues persist. The regulation of transcription factors can significantly influence the rate of fatty acid synthesis in plants. Consequently, several transcription factors associated with oil synthesis have been identified in recent years, among which the WRINKLED1 (WRI1) and V-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors play central roles. This study will explain how plants make essential lipids, bring up many unanswered questions, and describe the regulatory network of many transcription factors involved in oil production, with a focus on recent progress in research related to WRI1 and MYB1. The aim is to provide insights for the biological cultivation of high-quality oilseed crops.</p>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112428"},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SYMRK significantly affected AMF symbiosis and plant growth in maize

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-10 DOI: 10.1016/j.plantsci.2025.112427

Jing Zhou , Sha Lin , Xinhao Luo , Lixue Sun , Jin Chen , Beijiu Cheng , Xiaoyu Li

{"title":"SYMRK significantly affected AMF symbiosis and plant growth in maize","authors":"Jing Zhou , Sha Lin , Xinhao Luo , Lixue Sun , Jin Chen , Beijiu Cheng , Xiaoyu Li","doi":"10.1016/j.plantsci.2025.112427","DOIUrl":"10.1016/j.plantsci.2025.112427","url":null,"abstract":"<div><div>Arbuscular mycorrhizal fungi (AMF) are important symbiotic microorganisms in the soil that form reciprocal relationships with most plants to enhance their ability to absorb nutrients from the soil. The establishment of symbiosis between plants and AMF involves complex molecular mechanisms, and the SYMRK (Symbiosis receptor-like kinase) plays a pivotal role in the establishment of symbiosis. Maize (<em>Zea mays</em>) is a globally significant crop and one of the hosts for AMF, but research on AMF symbiosis-related genes in maize is limited. In this study, we identified a symbiosis receptor-like kinase in maize, named <em>ZmSYMRK</em>, which corresponds to the ortholog gene <em>OsSYMRK</em> in rice. ZmSYMRK encodes a cell membrane-localized protein kinase that is crucial for AMF colonization. We demonstrated that <em>ZmSYMRK</em> deletion resulted in severe defects in maize symbiosis with AMF. The colonization rates of <em>zmsymrk</em> mutants were significantly reduced at three different time points, and the colonization defects did not recover with prolonged colonization time. Furthermore, the deletion of the <em>ZmSYMRK</em> gene severely affected plant growth under low phosphorus conditions, and the growth defects of the mutants were even more pronounced after symbiosis. We conclude that <em>ZmSYMRK</em> plays a crucial role in both plant growth and the establishment of symbiotic relationships with AMF.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112427"},"PeriodicalIF":4.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143409897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ghβ-LCY1 influences metabolism and photosynthetic in Gossypium hirsutum

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-09 DOI: 10.1016/j.plantsci.2025.112417

Yanmin Qian , Yaping Wang , Yu Zhang , Zongyan Chu , Mengxin Shen , Cheng Zhang , Lihua Huang , Zhihua Yang , Kaiwen Ren , Yuanyuan Shi , Tingting Jiao , Baoting Yang , Qiuyue Meng , Yuchen Miao , Jinggong Guo

{"title":"Ghβ-LCY1 influences metabolism and photosynthetic in Gossypium hirsutum","authors":"Yanmin Qian , Yaping Wang , Yu Zhang , Zongyan Chu , Mengxin Shen , Cheng Zhang , Lihua Huang , Zhihua Yang , Kaiwen Ren , Yuanyuan Shi , Tingting Jiao , Baoting Yang , Qiuyue Meng , Yuchen Miao , Jinggong Guo","doi":"10.1016/j.plantsci.2025.112417","DOIUrl":"10.1016/j.plantsci.2025.112417","url":null,"abstract":"<div><div>Carotenoids are metabolites of isoprene, which are crucial roles for plant growth and response to abiotic stress. Lycopene β-cyclase (β-LCY) is a key protease in the synthesis pathway of plant carotenoid, playing an important role in the carotenoid metabolism and synthesis pathway. However, the function of β-LCY is almost unknown in cotton (<em>Gossypium spp.</em>). In this study, we cloned the <em>A</em> and <em>D</em> genomes of <em>β-LCY1</em> from upland cotton (<em>Gossypium hirsutum</em>), designated as <em>Ghβ-LCY1A</em> and <em>Ghβ-LCY1D</em>. We found that <em>Ghβ-LCY1A</em> and <em>Ghβ-LCY1D</em> were highly expressed in the cotton leaves and localized in the chloroplasts, respectively. The bacterial pigment complementarity experiment showed that Ghβ-LCY1 has the activity of β-LCY in <em>Escherichia coli</em>. The virus-induced gene silencing (VIGS) analysis exhibited that <em>Ghβ-LCY1</em> silencing cotton plants resulted in a spotted phenotype on the leaves and sepals, slow growth, and stunted plant growth in upland cotton. Additionally, the content of chlorophyll, carotenoids, antheranthun, zeaxanthin, violaxanthin and ABA, were significantly decreased. Under normal light intensity, the chloroplast ultrastructure of leaves in <em>Ghβ-LCY1</em> silencing cotton plants was abnormal, and their photosynthesis (leaf absorptance, Fv/Fm) and non-photochemical quenching (NPQ) were significantly lower than control cotton plants, and this difference was enhanced after high light treatment. Taken together, our results indicate that Ghβ-LCY1 plays an important role in carotenoids metabolism, photosynthesis and participates in plant growth and light protection in cotton.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112417"},"PeriodicalIF":4.2,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A TPR domain protein, OsTPR028, regulates grain size and weight in rice

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-07 DOI: 10.1016/j.plantsci.2025.112405

Zongfei Zhang , Xin Wang , Yi Bao, Huihui Wang, Xin Yan, Pengfei Liao, Shaobo Li, Jiexiu Ouyang

{"title":"A TPR domain protein, OsTPR028, regulates grain size and weight in rice","authors":"Zongfei Zhang , Xin Wang , Yi Bao, Huihui Wang, Xin Yan, Pengfei Liao, Shaobo Li, Jiexiu Ouyang","doi":"10.1016/j.plantsci.2025.112405","DOIUrl":"10.1016/j.plantsci.2025.112405","url":null,"abstract":"<div><div>Grain size and weight are critical determinants of rice yield and quality, yet their underlying genetic and regulatory mechanisms remain largely unexplored. In this study, we identified a protein containing TPR domain, named OsTPR028, which is localized in the cytoplasm. The gene encoding this protein is highly expressed during grain development. Homozygous <em>ostpr028</em> mutant exhibited significant reductions in grain size, grain filling rate, and grain weight, accompanied by decreased levels of starch, amylose, and lipids. Transcriptomic and qRT-PCR analyses demonstrated that <em>OsTPR028</em> regulates the expression of genes involved in starch biosynthesis and lipid transport. Protein interaction assays revealed that OsTPR028 interacts with an endosperm-specific protein, OsEnS45, whose knockout similarly resulted in reduced grain size and weight. Further investigations indicated that impaired spikelet cell division is the primary cause of these phenotypic defects in both <em>ostpr028</em> and <em>osens45</em> mutants. Together, our findings elucidate the critical role of the OsTPR028-OsEnS45 module in grain development and offer promising molecular targets for improving rice yield and quality through breeding programs.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112405"},"PeriodicalIF":4.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ABA-INSENSITIVE 4 promotes nicotine biosynthesis under high light in Nicotiana attenuata

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-05 DOI: 10.1016/j.plantsci.2025.112416

Bo Lei , Yan Mao , Huina Zhao , Jing Yu , Bing Wang , Ping Li , Xiangyang Hu

{"title":"ABA-INSENSITIVE 4 promotes nicotine biosynthesis under high light in Nicotiana attenuata","authors":"Bo Lei , Yan Mao , Huina Zhao , Jing Yu , Bing Wang , Ping Li , Xiangyang Hu","doi":"10.1016/j.plantsci.2025.112416","DOIUrl":"10.1016/j.plantsci.2025.112416","url":null,"abstract":"<div><div>Nicotine is a primary alkaloid-derived secondary metabolite found in tobacco (<em>Nicotiana</em> spp.). Excessive light exposure damages chloroplasts and enhances the production of protective secondary metabolites. However, the impact of high light (HL) on nicotine biosynthesis has not been thoroughly explored. We used a comprehensive array of physiological, biochemical, and transgenic analyses to elucidate the role of <em>abscisic acid (ABA)-insensitive 4 (NaABI4)</em> in HL-induced nicotine accumulation in wild tobacco (<em>Nicotiana attenuata</em>). NaABI4, which encodes a key mediator in the retrograde signaling pathway between the chloroplasts and nucleus, was found to induce <em>NaHY5</em> expression. NaHY5 acts as a long-distance mobile signal, activating <em>putrescine N-methyltransferase 1</em> (<em>NaPMT1</em>) and <em>quinolinate phosphoribosyl transferase</em> (<em>NaQPT</em>) genes, which are crucial for root nicotine biosynthesis. Moreover, NaABI4 activated the leaf-specific multidrug and toxic compound extrusion (MATE) transporters, <em>NaJAT1</em> and <em>NaJAT2,</em> facilitating nicotine translocation from the root to the leaf. Notably, <em>NaABI4</em> is activated by NaPTM, a PHD-type transcription factor with transmembrane domains that encodes a chloroplast envelope–bound transcription factor. These findings offer novel insights into NaABI4-mediated nicotine biosynthesis and reveal its coordination through NaPTM-dependent retrograde signaling under HL stress condition.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112416"},"PeriodicalIF":4.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Starvation from within: How heavy metals compete with essential nutrients, disrupt metabolism, and impair plant growth

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-05 DOI: 10.1016/j.plantsci.2025.112412

Abdul Wakeel Umar , Muhammad Naeem , Hamad Hussain , Naveed Ahmad , Ming Xu

{"title":"Starvation from within: How heavy metals compete with essential nutrients, disrupt metabolism, and impair plant growth","authors":"Abdul Wakeel Umar , Muhammad Naeem , Hamad Hussain , Naveed Ahmad , Ming Xu","doi":"10.1016/j.plantsci.2025.112412","DOIUrl":"10.1016/j.plantsci.2025.112412","url":null,"abstract":"<div><div>Nutrient starvation is a critical consequence of heavy metal toxicity, severely impacting plant health and productivity. This issue arises from various sources, including industrial activities, mining, agricultural practices, and natural processes, leading to the accumulation of metals such as aluminum (Al), arsenic (As), cadmium (Cd), chromium (Cr), lead (Pb), mercury (Hg), and nickel (Ni) in soil and water. Heavy metal exposure disrupts key physiological processes, particularly nutrient uptake and transport, resulting in nutrient imbalances within the plant. Essential nutrients are often unavailable or improperly absorbed due to metal chelation and interference with transporter functions, exacerbating nutrient deficiencies. This nutrient starvation, coupled with oxidative stress induced by heavy metals, manifests in impaired photosynthesis, stunted growth, and reduced crop yields. This review presents important insights into the molecular mechanisms driving nutrient deprivation in plants exposed to heavy metals, emphasizing the roles of transporters, transcription factors, and signaling pathways. It also examines the physiological and biochemical effects, such as chlorosis, necrosis, and altered metabolic activities. Lastly, we explore strategies to mitigate heavy metal-induced nutrient starvation, including phytoremediation, soil amendments, genetic approaches, and microbial interventions, offering insights for enhancing plant resilience in contaminated soils.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112412"},"PeriodicalIF":4.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143351020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complementation with TaNCL2-A reinstates growth and abiotic stress response in atncl mutant of Arabidopsis

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-04 DOI: 10.1016/j.plantsci.2025.112411

Ishu, Shumayla, Madhu, Santosh Kumar Upadhyay

{"title":"Complementation with TaNCL2-A reinstates growth and abiotic stress response in atncl mutant of Arabidopsis","authors":"Ishu, Shumayla, Madhu, Santosh Kumar Upadhyay","doi":"10.1016/j.plantsci.2025.112411","DOIUrl":"10.1016/j.plantsci.2025.112411","url":null,"abstract":"<div><div>The sodium/calcium exchanger-like (NCL) transporters are members of Ca<sup>2 +</sup>/Cation antiporters (CaCAs) family, localized at the tonoplast, and primarily involved in Ca<sup>2+</sup> homeostasis and stress response. They transport Ca<sup>2+</sup> to the cytosol and sequester cytosolic Na<sup>+</sup> into the vacuole. Therefore, the <em>atncl</em> mutant of <em>Arabidopsis thaliana</em> is prone to salinity stress. The functional complementation of <em>TaNCL2-A</em> of <em>Triticum aestivum</em> improved abiotic stress response and various morpho-physio-biochemical parameters in <em>atncl</em> mutant. The <em>TaNCL2-A</em> complementation increased the seed germination rate and root length of <em>atncl</em> mutant during salinity and drought stress conditions. The exogenous Ca<sup>2+</sup> application further improved the stress tolerance in the complemented lines. The results suggested that the modulation of cytosolic Ca<sup>2+</sup> by <em>TaNCL2-A</em> expression and/or exogenous Ca<sup>2+</sup> application could reinstate growth and abiotic stress response in <em>atncl</em> mutant. <em>TaNCL2-A</em> also reduced the impact of ABA on seed germination. In addition, exogenous IAA induced lateral roots formation in all the lines. Biochemical and physiological analyses revealed increased proline, chlorophylls, carotenoids and relative water content (RWC), and reduced malondialdehyde (MDA), H<sub>2</sub>O<sub>2</sub> and relative electrical conductivity (REC) in <em>TaNCL2-A</em> complemented lines. The results highlighted the function of <em>TaNCL2-A</em> gene in stress response, and its potential application in crop improvement strategies in future studies.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112411"},"PeriodicalIF":4.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic insights into DXO1 and XRN3: regulatory roles of RNA stability, transcription, and liquid-liquid phase separation in Arabidopsis thaliana (L.) Heynh.

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-03 DOI: 10.1016/j.plantsci.2025.112413

Mostafakamal Shams , Ali Khadivi

{"title":"Mechanistic insights into DXO1 and XRN3: regulatory roles of RNA stability, transcription, and liquid-liquid phase separation in Arabidopsis thaliana (L.) Heynh.","authors":"Mostafakamal Shams , Ali Khadivi","doi":"10.1016/j.plantsci.2025.112413","DOIUrl":"10.1016/j.plantsci.2025.112413","url":null,"abstract":"<div><div>The regulation of RNA stability and transcription in eukaryotic organisms is a sophisticated process involving various complex mechanisms. This paper explores the regulatory functions of DXO1 and XRN3 proteins in RNA stability and transcription in the model plant <em>Arabidopsis thaliana</em> (L.) Heynh. DXO1 is noted for its roles in mRNA 5′-end quality control, removal of non-canonical NAD<sup>+</sup> caps, and activation of RNA guanosine-7 methyltransferase. In contrast, XRN3 ensures RNA integrity through precise degradation. While current studies have identified various termination regions across genes influenced by XRN3, advanced RNA sequencing techniques have revealed that XRN3-mediated changes in gene expression often result from siRNA production, leading to gene silencing rather than direct effects on transcription termination. This review emphasizes the need to further explore the DXO1-XRN3 axis, their interactive mechanisms, and their potential involvement in liquid-liquid phase separation (LLPS) during transcription. It further suggests evaluating XRN proteins like XRN4 to assess potential redundancies in RNA degradation pathways. The advent of PSPredictor, a tool for identifying LLPS proteins, along with protein function prediction techniques, promises to advance our understanding of DXO1 and XRN3 in maintaining RNA equilibrium and the dynamics of LLPS in plant biology. The review concludes by calling for more studies on the plant-specific roles of the DXO1 N-terminal extension (NTE), predictive tools for LLPS-forming proteins, and the interplay of RNA Pol II CTD code modulation by transcription factors to enhance knowledge of plant stress adaptation and improve agricultural productivity.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112413"},"PeriodicalIF":4.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of nitric oxide and nitrogen in mediating copper stress in Brassica juncea L.

IF 4.2 2区生物学

Plant Science Pub Date : 2025-02-03 DOI: 10.1016/j.plantsci.2025.112414

Bilal A. Rather , Asim Masood , Fei Qiao , Xuefei Jiang , Muhammad Mubashar Zafar , Hanqing Cong , Nafees A. Khan

{"title":"The role of nitric oxide and nitrogen in mediating copper stress in Brassica juncea L.","authors":"Bilal A. Rather , Asim Masood , Fei Qiao , Xuefei Jiang , Muhammad Mubashar Zafar , Hanqing Cong , Nafees A. Khan","doi":"10.1016/j.plantsci.2025.112414","DOIUrl":"10.1016/j.plantsci.2025.112414","url":null,"abstract":"<div><div>Copper (Cu) holds a significant importance in plant metabolism as it serves as an essential micronutrient but becomes toxic at higher concentrations. Nitric oxide (NO), a key signaling molecule, and nitrogen (N) play essential roles in combating toxicity of some metals. This study explores the potential of interactive effects of NO as 100 µM SNP (sodium nitroprusside, NO source) and N (80 mg N kg<sup>−1</sup> soil) in mitigating Cu (100 mg Cu kg<sup>−1</sup> soil) stress in mustard (<em>Brassica juncea</em> L.) plants. The impaired physio-biochemical changes, photosynthetic efficiency, and the expression level of genes associated with photosynthesis, and N assimilation under Cu stress were ameliorated with the exogenous application of NO and N. The combined treatment of NO and N conspicuously lowered reactive oxygen species (ROS) and its related impacts. It also enhanced the activity and relative expression of antioxidant enzymes, including ascorbate peroxidase (APX), glutathione reductase (GR), and superoxide dismutase (SOD) as well as N assimilation enzymes, such as nitrate reductase (NR) and nitrite reductase (NiR). The supplementation of NO and N also triggered the expression of <em>rbcL</em> (large subunit of Rubisco), photosystem (photosystem II D1 protein<em>; psbA</em> and photosystem II protein B; <em>psbB</em>) and markedly improved photosynthetic capacity under Cu stress. The study highlights the significance of NO and N as a potential strategy to counteract Cu-induced stress in crops. It suggests a synergistic or interactive effect between the two substances as a phytoremediation strategy for enhancing crop growth and productivity in Cu-contaminated soils. Understanding the mechanisms behind NO and N mediated stress alleviation could facilitate the development of targeted approaches to enhance plant resilience against heavy metal stress.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112414"},"PeriodicalIF":4.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0