Plant Science最新文献

Rice glycosyltransferase DUGT2 enhances drought and salt tolerances through glycosylating a broad-spectrum of flavonoids under bZIP16 regulation. 水稻糖基转移酶DUGT2在bZIP16的调控下，通过糖基化广谱黄酮类化合物，增强水稻的耐旱性和耐盐性。

IF 4.1 2区生物学

Plant Science Pub Date : 2025-07-31 DOI: 10.1016/j.plantsci.2025.112692

Guangrui Dong, Yuqing Ma, Shuman Zhao, Xinmei Ma, Chonglin Liu, Yi Ding, Jiarun Wu, Bingkai Hou

{"title":"Rice glycosyltransferase DUGT2 enhances drought and salt tolerances through glycosylating a broad-spectrum of flavonoids under bZIP16 regulation.","authors":"Guangrui Dong, Yuqing Ma, Shuman Zhao, Xinmei Ma, Chonglin Liu, Yi Ding, Jiarun Wu, Bingkai Hou","doi":"10.1016/j.plantsci.2025.112692","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112692","url":null,"abstract":"<p><p>UDP-glycosyltransferase (UGT) constitute a superfamily and get dramatic expansion during evolution of land plants. However, their biological significances in stress responses are largely unclear. In addition, it is well known that accumulation of flavonoids enable plants to cope with stressful environments, but the molecular mechanism underlying metabolism regulation of flavonoids under stress conditions remains unanswered. Here, we analyzed the impact of a rice UGT gene, DUGT2, on flavonoid metabolism under stress conditions by using genetic, biochemical, molecular, and omics methods. We found that overexpression of DUGT2 enhanced plant tolerance to drought and salt, while DUGT2 knockout mutants showed a more sensitive phenotype. Our metabolomics analysis showed that the mutants had the most significant reduction in flavonoids under stress conditions compared to the wild type. Subsequent biochemical analysis verified a broad-spectrum enzyme activity of DUGT2 toward flavonoids, suggesting that DUGT2 can affect flavonoid metabolism by flavonoid glycosylation. Furthermore, our transcriptome analysis revealed that the loss of DUGT2 function led to a significant decrease in the expression of genes related to flavonoid biosynthesis and antioxidant enzymes, which might be achieved through feedback regulation. Moreover, we identified an upstream regulator, bZIP16, and demonstrated its positive regulation on DUGT2 transcription activity. Overall, this work reveals that DUGT2 could enhance abiotic stress tolerance by the glycosylation-promoted flavonoid metabolism under the regulation of bZIP16. This work can not only help to understand the biological functions of UGT superfamily in stress responses, but also get a new insight into the regulation mechanism of flavonoid metabolism, especially under stress conditions.</p>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112692"},"PeriodicalIF":4.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subfunctionalization, neofunctionalization, and nonfunctionalization of the four soybean phytochrome A genes. 四种大豆光敏色素A基因的亚功能化、新功能化和非功能化。

IF 4.1 2区生物学

Plant Science Pub Date : 2025-07-31 DOI: 10.1016/j.plantsci.2025.112691

Lixin Ma, Ting Zhang, Sujun Ye, Wenmin Lin, Yinhua Lv, Wenmin Liu, Fanjiang Kong, Baohui Liu, Yang Tang, Xiaoya Lin

{"title":"Subfunctionalization, neofunctionalization, and nonfunctionalization of the four soybean phytochrome A genes.","authors":"Lixin Ma, Ting Zhang, Sujun Ye, Wenmin Lin, Yinhua Lv, Wenmin Liu, Fanjiang Kong, Baohui Liu, Yang Tang, Xiaoya Lin","doi":"10.1016/j.plantsci.2025.112691","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112691","url":null,"abstract":"<p><p>Gene duplication generates new genes, which retain their original function or undergo subfunctionalization, neofunctionalization, or nonfunctionalization. The phytochrome A (PHYA) genes in soybean (Glycine max) have undergone duplication to produce GmPHYA1, GmPHYA2 (E4), GmPHYA3 (E3), and GmPHYA4, each with distinct evolutionary fates. Using genetic and biochemical analyses, we discovered that GmPHYA1 has undergone subfunctionalization and is essential for regulating photomorphogenesis and plant height in soybean. GmPHYA2 has experienced both subfunctionalization and neofunctionalization, as it regulates flowering time under far red-enriched light and red-enriched light. GmPHYA3 has undergone neofunctionalization; despite losing some ancestral functions, it has gained two characteristics not observed in Arabidopsis thaliana PHYA: protein stability in red light and regulation of flowering-time under red-enriched light. GmPHYA4, which lacks a key phyA domain and has lost all functionality, is considered a pseudogene. These findings demonstrate the varied outcomes of the duplication of soybean GmPHYA genes.</p>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112691"},"PeriodicalIF":4.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PYL2 regulates drought and blast (Magnaporthe oryzae) resistance in rice PYL2调节水稻抗旱性和稻瘟病抗性

IF 4.1 2区生物学

Plant Science Pub Date : 2025-07-30 DOI: 10.1016/j.plantsci.2025.112690

Zhensheng Qiao , Yimin Zhao , Xiaorui Wang, Yuquan Fu, Chuming chen, Xuezhu Du, Feng Sheng

{"title":"PYL2 regulates drought and blast (Magnaporthe oryzae) resistance in rice","authors":"Zhensheng Qiao , Yimin Zhao , Xiaorui Wang, Yuquan Fu, Chuming chen, Xuezhu Du, Feng Sheng","doi":"10.1016/j.plantsci.2025.112690","DOIUrl":"10.1016/j.plantsci.2025.112690","url":null,"abstract":"<div><div>The ABA receptor PYL/RCAR plays a crucial role in initiating ABA sensing and signal transduction, which is essential for plant stress resistance. However, the role of <em>OsPYL2</em> in rice drought and blast tolerance remains largely unexplored. In this study, we investigated the function of <em>OsPYL2</em> in drought and rice blast. Overexpression of <em>OsPYL2</em> resulted in stronger drought tolerance by increasing photosynthetic pigment content, water-holding capacity in rice leaves, while significantly decreasing MDA content. Additionally, overexpression of <em>OsPYL2</em> could also increase the resistance to rice blast by promoting the accumulation of H<sub>2</sub>O<sub>2</sub>. In contrast, loss of <em>OsPYL2</em> function resulted in the opposite effects. And, <em>PYL2</em> also regulates hormone content by affecting the expression of ABA and JA signaling pathway genes in response to stress environment. Results of yeast two-hybrid assay, bimolecular fluorescence complementation and firefly luciferase complementation revealed that OsPYL2 could interact with PP2C proteins, VQ24 protein and JAZ proteins. These results illustrated that <em>OsPYL2</em> can integrate dual stress tolerance responses by synergistically regulating ABA and JA signaling pathways. This study provides valuable insights underlying drought and blast tolerance in rice.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112690"},"PeriodicalIF":4.1,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144748788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic profiling of three Brachypodium species reveals different adaptive strategies to ammonium stress. 3种短柄植物的代谢特征揭示了它们对铵胁迫的不同适应策略。

IF 4.1 2区生物学

Plant Science Pub Date : 2025-07-29 DOI: 10.1016/j.plantsci.2025.112684

Marlon De la Peña, Izargi Vega-Mas, Gaëtan Glauser, Yves Gibon, Daniel Marino, María Begoña Gonzalez-Moro

{"title":"Metabolic profiling of three Brachypodium species reveals different adaptive strategies to ammonium stress.","authors":"Marlon De la Peña, Izargi Vega-Mas, Gaëtan Glauser, Yves Gibon, Daniel Marino, María Begoña Gonzalez-Moro","doi":"10.1016/j.plantsci.2025.112684","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112684","url":null,"abstract":"<p><p>Nitrogen (N) use efficiency (NUE) in crops is a critical challenge, as only 40% of applied nitrogen is typically recovered at harvest. Ammonium-based nutrition is proposed as a strategy to increase NUE in agrosystems. However, crops display better performance when growing with a nitrate-based nutrition. To advance in the current understanding of the interspecific variability to ammonium nutrition, we investigated the performance of the three annual species of Brachypodium genus: B. distachyon, B. stacei, and B. hybridum, focusing on their differential responses to ammonium and nitrate nutrition. B. stacei appeared as a tolerant species, with equal growth regardless of the N source, and B. distachyon as the most sensitive, while B. hybridum showed an intermediate phenotype. Metabolomic analysis highlighted critical differences in N metabolism, where B. stacei and B. hybridum exhibited more robust N assimilation in terms of protein content. In addition, several metabolic pathways were found associated with ammonium nutrition. Notably, flavonoid biosynthesis and tricarboxylic acid cycle pathways together with ethylene precursors and iron homeostasis-related compounds contributed to explain species-specific responses to ammonium nutrition. Altogether, these findings pinpoint potential strategies for improving N utilization and ammonium stress tolerance in cereals.</p>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112684"},"PeriodicalIF":4.1,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144761117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cotton gene family encoding translationally controlled tumor proteins and the role of GhTCTP5 in salt tolerance 棉花基因家族编码翻译控制肿瘤蛋白和GhTCTP5在耐盐性中的作用

IF 4.1 2区生物学

Plant Science Pub Date : 2025-07-29 DOI: 10.1016/j.plantsci.2025.112689

Bo Pang , Jialin Chen , Ping Luo , Ru Zhang , Jing Li , Weiwei Liang , Wenwei Gao , Shengmei Li

{"title":"The cotton gene family encoding translationally controlled tumor proteins and the role of GhTCTP5 in salt tolerance","authors":"Bo Pang , Jialin Chen , Ping Luo , Ru Zhang , Jing Li , Weiwei Liang , Wenwei Gao , Shengmei Li","doi":"10.1016/j.plantsci.2025.112689","DOIUrl":"10.1016/j.plantsci.2025.112689","url":null,"abstract":"<div><div>Translationally controlled tumor proteins (TCTPs) are highly conserved proteins found in virtually all eukaryotes, and their expression is intricately linked with numerous biochemical processes and cellular activities. However, intriguingly, within the realm of key cash crops, investigations of the <em>TCTP</em> gene in cotton are relatively limited. This study sought to elucidate the evolutionary relationships and structural functions of the <em>TCTP</em> gene in cotton with a whole-genome analysis approach. Within <em>Gossypium arboreum</em> (<em>G. arboreum)</em>, <em>Gossypium raimondii</em> (<em>G. raimondii)</em>, <em>Gossypium barbadense</em> (<em>G. barbadense)</em>, and <em>Gossypium hirsutum</em> (<em>G. hirsutum)</em>, we found three, three, six, and five TCTP domain sequences, respectively. A detailed examination of their genetic architecture and evolutionary history revealed a strikingly conserved progression among TCTP family members. Notably, segmental duplications emerged as a pivotal mechanism in the expansion of the cotton TCTP family, with virtually no evidence of tandem duplications. The assessment of the <em>cis</em>-acting elements within the <em>TCTP</em> gene promoters suggested that these genes might be influenced by plant hormones and various environmental stresses. By applying virus-induced gene silencing (VIGS) and RNA-seq, we determined that <em>GhTCTP5</em> plays a critical role. Specifically, the response of cotton to salt stress is most likely regulated by its participation in the phenylpropanoid metabolic synthesis pathway, which adds to our understanding of its functional complexity. These results provide insights for understanding the evolution of <em>TCTP</em> genes in cotton, thus establishing a robust foundation for the subsequent exploitation of these genes to increase crop salt tolerance.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112689"},"PeriodicalIF":4.1,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144756887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid-mediated photoprotection confers strong light resilience in hyperaccumulator Sedum alfredii 脂质介导的光保护赋予了超蓄积体景天较强的光恢复能力

IF 4.1 2区生物学

Plant Science Pub Date : 2025-07-29 DOI: 10.1016/j.plantsci.2025.112688

Yanjiao Dai , Jin Chen , Yang Mao , Huidan Jiang , Wenwen Zhao , Jing Peng , Ruibing Li , Ying Wang , Purui Guo , Mengnan Zhang , Chao Zhang , Jiaming Li , Song Sheng

{"title":"Lipid-mediated photoprotection confers strong light resilience in hyperaccumulator Sedum alfredii","authors":"Yanjiao Dai , Jin Chen , Yang Mao , Huidan Jiang , Wenwen Zhao , Jing Peng , Ruibing Li , Ying Wang , Purui Guo , Mengnan Zhang , Chao Zhang , Jiaming Li , Song Sheng","doi":"10.1016/j.plantsci.2025.112688","DOIUrl":"10.1016/j.plantsci.2025.112688","url":null,"abstract":"<div><div>The heavy metal hyperaccumulator <em>Sedum</em> is limited by sensitivity to intense sunlight. This study compared light-tolerant <em>Sedum alfredii</em> and light-sensitive <em>Sedum plumbizincicola</em> under light intensities (600–1400 μmol m⁻² s⁻¹). <em>Sedum plumbizincicola</em> exhibited complete mortality at 1400 μmol m⁻² s⁻¹ after six days, while <em>Sedum alfredii</em> showed minimal to moderate mortality. Physiological analyses revealed <em>Sedum alfredii</em> maintained higher Rubisco activity and reduced membrane damage under stress. Lipidomics identified elevated ceramides and glycerophospholipids in <em>Sedum alfredii</em>, enhancing membrane stability. Transcriptomics detected 6501 shared differentially expressed genes (DEGs) under strong light, with 977 unique to <em>Sedum alfredii</em>, implicating chloroplast function, protein phosphorylation, and defense responses in its resilience. Weighted Gene Co-expression Network Analysis highlighted the MEturquoise module, linked to lipid metabolism and stress responses. <em>Sedum alfredii</em> displayed a surge in DEGs at 1200/1400 μmol m⁻² s⁻¹ , activating microtubule reorganization, cytokinin signaling, and circadian regulation. Notably, DN1992 gene expression in <em>Sedum alfredii</em> under high light stress significantly exceeded <em>Sedum plumbizincicola</em>, suggesting its role in differential stress tolerance. The coordinated interplay of lipid metabolism, gene regulation, and hormonal adaptation underpins <em>Sedum alfredii</em> enhanced light tolerance. These findings advance mechanistic insights into <em>Sedum</em> photo stress adaptation and provide targets for breeding robust hyperaccumulators for ecological restoration in sun-exposed contaminated environments.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112688"},"PeriodicalIF":4.1,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144756886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on gene and regulatory mechanism of drought resistance in rice. 水稻抗旱性基因及其调控机制研究进展。

IF 4.1 2区生物学

Plant Science Pub Date : 2025-07-28 DOI: 10.1016/j.plantsci.2025.112687

Nuo Gong, Yingchun Han, Ziwen Wang, Ya Wang, Yanxiu Du, Zhen Chen, Hongzheng Sun, Junzhou Li

{"title":"Research progress on gene and regulatory mechanism of drought resistance in rice.","authors":"Nuo Gong, Yingchun Han, Ziwen Wang, Ya Wang, Yanxiu Du, Zhen Chen, Hongzheng Sun, Junzhou Li","doi":"10.1016/j.plantsci.2025.112687","DOIUrl":"https://doi.org/10.1016/j.plantsci.2025.112687","url":null,"abstract":"<p><p>Global warming and uneven distribution of rainfall have led to frequent drought events. Drought is a major obstacle affecting rice production; therefore, it is crucial to elucidate the drought regulatory mechanisms and breed drought-resistant and water-saving rice varieties. With the rapid development of molecular biology tools, numerous drought-related genes have been identified and cloned. At present, numerous genes related to osmotic regulation, antioxidant scavenging, phytohormones, root development, stoma and cuticle regulation have been found to improve drought resistance. This review summarizes the research progress in the cloning of drought tolerance and avoidance genes in rice, their functional characterization and validation.It reveals that current studies on drought-resistance molecular pathways and the identification of drought-resistant genes remain limited. More critically, only a few genes have been tested for drought resistance under field conditions and shown to enhance both drought resistance and yield in field environments. In the future, greater emphasis should be placed on discovering drought-resistant genes from upland rice resources. Additionally, rigorous testing and evaluation of these genes under field drought conditions are essential to assess their breeding potential, thereby advancing molecular breeding for drought resistance.</p>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112687"},"PeriodicalIF":4.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144754099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review: Adaptation of plants to phosphorus scarcity: From nutritional crosstalk to organellar function 综述：植物对磷缺乏的适应：从营养串扰到细胞器功能

IF 4.1 2区生物学

Plant Science Pub Date : 2025-07-26 DOI: 10.1016/j.plantsci.2025.112685

Jesús Salvador López Bucio , Javier Raya González , José López Bucio

{"title":"Review: Adaptation of plants to phosphorus scarcity: From nutritional crosstalk to organellar function","authors":"Jesús Salvador López Bucio , Javier Raya González , José López Bucio","doi":"10.1016/j.plantsci.2025.112685","DOIUrl":"10.1016/j.plantsci.2025.112685","url":null,"abstract":"<div><div>Plants respond to phosphorus scarcity by adjusting root architecture and activating physiological and biochemical processes aimed at optimizing the uptake, transport, and efficient use of this nutrient. Phosphate, the main phosphorus available form is perceived in the root cap in a process involving several molecular components, including the transcription factor SOMBRERO and bacterial-type ferroxidases that enhance the uptake and transport of iron, whose accumulation triggers the production of reactive oxygen species, stops mitosis and halts root growth. In this process, auxins, cytokinins, jasmonic acid, abscisic acid and the neurotransmitter γ-aminobutyric acid orchestrate the formation of root hairs and lateral roots as well as the expression of Pi transporters in roots and anthocyanins in leaves. Low Pi-sensing genes can be classified as drivers or blockers of the root growth repression elicited by Pi scarcity, the drivers encode nitrate-inducible transcription factors, the STOP1-ALMT1 module controlling root exudation of malate, proteins mediating Fe uptake and distribution and MEDIATOR subunit 16. Cations such as Fe and Al trigger accumulation of the transcription factor STOP1 in nuclei to modulate the transcriptional response and promote malate exudation, implying a direct link between cation-Pi complexes at the rhizosphere and root morphogenesis. General cell maintenance mechanisms that acclimate plants during root meristem consumption have been identified including SUMOylation, endoplasmic reticulum stress, proteostasis, autophagy and signaling mediated by MPK6 and MPK4 kinases. These mechanisms unveil the complexity of the signaling pathways to favor plant adaptation and survival when a critical macronutrient is limiting.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112685"},"PeriodicalIF":4.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous cropping obstacles in medicinal plants: Driven by soil microbial communities and root exudates. A review 药用植物连作障碍：由土壤微生物群落和根系分泌物驱动。回顾

IF 4.1 2区生物学

Plant Science Pub Date : 2025-07-26 DOI: 10.1016/j.plantsci.2025.112686

Muhammad Zeeshan Ul Haq, Zeqi Bai, Guangtao Gu, Ya Liu, Dongmei Yang, Huageng Yang, Jing Yu, Yougen Wu

{"title":"Continuous cropping obstacles in medicinal plants: Driven by soil microbial communities and root exudates. A review","authors":"Muhammad Zeeshan Ul Haq, Zeqi Bai, Guangtao Gu, Ya Liu, Dongmei Yang, Huageng Yang, Jing Yu, Yougen Wu","doi":"10.1016/j.plantsci.2025.112686","DOIUrl":"10.1016/j.plantsci.2025.112686","url":null,"abstract":"<div><div>The rhizosphere is a hub for information and material exchange between plants, microbes, and soil, crucial for plant growth. This study synthesises advancements in understanding rhizospheric microbial communities associated with medicinal plants, with a focus on root exudates and their interactions. It examines the complex genetic, biochemical, and metabolic relationships within plant microbiomes, which enhance plant growth, stress tolerance, and resistance to pathogens. To address the challenges posed by continuous cropping (CC) in medicinal plant cultivation, we comprehensively reviewed the impacts of root exudates and rhizosphere microbial communities. The analysis examines how these interactions impact microbiome assembly, nutrient acquisition, and overall plant vigour, while identifying key areas for future investigation. In CC systems, medicinal plants release secondary metabolites as a defense response to stress, which accumulate in the soil and cause autotoxicity. Elevated autotoxin levels exert a more substantial growth-inhibitory effect, varying across plant species. These autotoxins disrupt the balance of rhizospheric microbial communities, increasing the prevalence of pathogenic microbes while reducing the populations of beneficial microorganisms. Drawing on insights from plant microbiome research, this study elucidates the intricate metabolic interplay among plants, their associated microbes, and the surrounding environment. It further examines how these interactions influence the composition of plant-associated microbiomes, enhance nutrient uptake, and promote plant health. Knowledge gaps are highlighted, and directions for future research are proposed to advance understanding of the dynamic interactions between medicinal plants and their rhizospheric microbial communities.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112686"},"PeriodicalIF":4.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide characterization of the Pyrabactin Resistance 1-like (PYL) gene family in wheat and functional identification of TaPYL2D 小麦Pyrabactin抗性1样（PYL）基因家族的全基因组特征及TaPYL2D的功能鉴定

IF 4.1 2区生物学

Plant Science Pub Date : 2025-07-25 DOI: 10.1016/j.plantsci.2025.112681

Ying Wang , Lili Wang , Yaoyu Wang , Longyang Xia , Shaozhe Li , Keli Dai , Weiping Shi , Jingye Cheng , Jie Guo

{"title":"Genome-wide characterization of the Pyrabactin Resistance 1-like (PYL) gene family in wheat and functional identification of TaPYL2D","authors":"Ying Wang , Lili Wang , Yaoyu Wang , Longyang Xia , Shaozhe Li , Keli Dai , Weiping Shi , Jingye Cheng , Jie Guo","doi":"10.1016/j.plantsci.2025.112681","DOIUrl":"10.1016/j.plantsci.2025.112681","url":null,"abstract":"<div><div>The <em>pyrabactin resistance 1-like</em> (<em>PYL</em>) gene family encodes proteins that regulate drought tolerance and ABA signalling in plants. These proteins act as ABA receptors and play critical roles in abiotic stress response. The importance of studying the <em>PYL</em> gene family in wheat lies in its regulation of drought tolerance, a significant limiting factor in wheat production. Understanding their functions and expression patterns can lead to the breeding of more resilient varieties and enhance global food security. In this study, we discovered 890 PYL homologues in 59 plant species. The <em>PYL</em> gene family has undergone substantial expansion throughout plant evolution. There are 38 PYLs in wheat, and the phylogenetic relationships between wheat PYLs are supported by conserved motifs and gene structures. Evolutionary analyses of <em>Triticum aestivum</em> and its ancestral species <em>PYL</em> genes have revealed complex patterns of gene duplication, gene loss, and chromosomal rearrangements that have shaped the evolution of wheat PYLs. The expression of wheat <em>PYL</em> genes is primarily observed in the root tissues and is responsive to both ABA and drought stress. Furthermore, TaPYL2D is localized to both the cell membrane and the nucleus, and it exhibits protein-protein interactions with TaABI1. Additionally, overexpressing <em>TaPYL2D</em> in <em>Arabidopsis</em> results in increased sensitivity to ABA and enhanced osmotic tolerance, highlighting its functional conservation. Our findings and those in the literature highlight the multifaceted roles of wheat PYLs. Overall, our study provides a refined characterisation of wheat PYLs and broadens the understanding of <em>PYL</em> genes.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112681"},"PeriodicalIF":4.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0