Plant Science最新文献

Phosphorus modulates starch granule development and metabolic partitioning in wheat grain: Insights from SGAP proteomics and nutrition and processing quality. 磷调节小麦籽粒淀粉粒发育和代谢分配：来自SGAP蛋白质组学和营养与加工品质的见解

IF 4.6 2区生物学

Plant Science Pub Date : 2026-11-01 Epub Date: 2026-08-10 DOI: 10.1016/j.plantsci.2026.113372

Jialian Wei, Xiangchi Zhang, Kaiyong Fu, Gang Li, Cheng Li, Chunyan Li

{"title":"Phosphorus modulates starch granule development and metabolic partitioning in wheat grain: Insights from SGAP proteomics and nutrition and processing quality.","authors":"Jialian Wei, Xiangchi Zhang, Kaiyong Fu, Gang Li, Cheng Li, Chunyan Li","doi":"10.1016/j.plantsci.2026.113372","DOIUrl":"10.1016/j.plantsci.2026.113372","url":null,"abstract":"This study investigates how phosphorus (P) levels are associated with carbon-nitrogen metabolism in wheat grains. Optimal P application (105 kg P₂O₅ ha⁻¹) was associated with enhanced pericarp-endosperm coordination, increased carbon allocation to the endosperm, and early B‑type starch granule formation. Starch granule‑associated protein (SGAP) proteomics showed that optimal P upregulated cytoskeletal and starch‑synthesis proteins bound to starch granules in the endosperm, while reducing storage protein degradation‑related SGAPs in the pericarp. These metabolic adjustments were correlated with increased grain‑filling intensity and duration, and were associated with the highest theoretical grain weight (50.70 mg). Furthermore, optimal P was associated with enrichment of amino acid biosynthesis pathways and with higher levels of essential amino acids (e.g., lysine and threonine by 17.0--26.8%) and an improved essential amino acid profile without altering total protein content. In contrast, excessive P (210 kg P₂O₅ ha⁻¹) was associated with disrupted inter‑tissue coordination but did not simply impair grain filling; instead, HP corresponded to a unique developmental program: it was linked to an early burst of C‑type starch granules (0∼5 µm) at 7 DPA, yet by maturity achieved the highest proportion of large A‑type granules (56.8%) and the highest total starch content (63.5%), together with elevated endosperm phosphorus at 14 DPA and enrichment of spliceosome‑related pathways. HP also showed higher levels of several functional amino acids (glutamate, cysteine, histidine, proline) compared to P0. However, HP was associated with a higher gliadin/globulin ratio and did not improve grain yield. These findings suggest that phosphorus supply is associated with grain quality through tissue‑specific metabolic reprogramming, and that precision management-rather than maximized application-warrants consideration for optimizing both yield and processing quality.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"113372"},"PeriodicalIF":4.6,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148707290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Genome-wide identification of the aminotransferase gene family in wheat and functional characterization of the TaOBF1B-TaNAAT1 module in salt tolerance" [Plant Sci. 373 (2026) 113440]. 小麦转氨酶基因家族的全基因组鉴定及TaOBF1B-TaNAAT1模块在耐盐性中的功能鉴定[j].植物科学，373(2026):113440。

IF 4.6 2区生物学

Plant Science Pub Date : 2026-10-19 DOI: 10.1016/j.plantsci.2026.113513

Ke-Xin Niu, Yun-Qing He, Xiao-Hang Xi, Yuxin Ma, Hui Gao, Hao-Yu Wang, Xiao-Yao Dang, Shi-Yu Sun, Zhen-Ming Pei

引用次数: 0

Genome-wide identification of the TIFY gene family in Sorghum bicolor and functional characterization of SbTIFY9 in salt stress tolerance. 高粱双色植物TIFY基因家族的全基因组鉴定及SbTIFY9在耐盐胁迫中的功能鉴定

IF 4.6 2区生物学

Plant Science Pub Date : 2026-10-18 DOI: 10.1016/j.plantsci.2026.113531

Zhongzhong Chen, Songshu Chen, Yanqing Ding, Wanting Yang, Jinmei Lu, Yuxia An, Changju Li, Jian Xu, Kuiyin Li, Mingjian Ren

{"title":"Genome-wide identification of the TIFY gene family in Sorghum bicolor and functional characterization of SbTIFY9 in salt stress tolerance.","authors":"Zhongzhong Chen, Songshu Chen, Yanqing Ding, Wanting Yang, Jinmei Lu, Yuxia An, Changju Li, Jian Xu, Kuiyin Li, Mingjian Ren","doi":"10.1016/j.plantsci.2026.113531","DOIUrl":"10.1016/j.plantsci.2026.113531","url":null,"abstract":"The TIFY gene family is a plant-specific group of transcriptional regulators characterized by a conserved TIFY domain (TIF[F/Y]XG), participating in plant development, phytohormone signaling and abiotic stress responses. In this study, 21 SbTIFY genes were genome-widely identified in sorghum (Sorghum bicolor L.). According to phylogeny and protein structures, SbTIFY proteins were grouped into JAZ and ZML subfamilies, and the JAZ subfamily was further divided into five clades. These proteins contain 101-429 amino acids and are unevenly distributed across six chromosomes. Genes from the same subgroup possess similar exon-intron structures and conserved motifs. Promoter analysis uncovered abundant cis-elements associated with hormone signaling, stress responses and developmental processes. Synteny analysis detected seven orthologous pairs between sorghum and Arabidopsis, and 25 pairs between sorghum and rice (Oryza sativa). Expression profiling revealed that multiple SbTIFY members exhibited differential expression across developmental organs and under stresses including anthracnose infection, cold, heat, flooding, and salt. SbTIFY9 displayed strong induction under multiple stresses: its transcript level increased approximately 10-fold at 12 h and approximately 31-fold at 24 h of salt treatment, implying its vital function in salt response. Subcellular localization confirmed SbTIFY9 resides mainly in the nucleus. Heterologous overexpression of SbTIFY9 in Arabidopsis improved true leaf expansion, fresh weight at germination, and root growth and biomass at seedling stage under 125 and 150 mM NaCl, demonstrating its positive role in salt tolerance. This work provides insights into the evolution and stress functions of sorghum TIFY family.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"113531"},"PeriodicalIF":4.6,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-coding RNA levels integrating analysis revealed the salt and drought stress response mechanism of miR395e-PvUXS1 pathway in switchgrass. 非编码RNA水平整合分析揭示了柳枝稷miR395e-PvUXS1通路盐胁迫和干旱胁迫的响应机制。

IF 4.6 2区生物学

Plant Science Pub Date : 2026-10-17 DOI: 10.1016/j.plantsci.2026.113533

Cong Guan, Tingting Wang, Chu Wang, Bo Wu, Zichen Zou, Minli Xu, Xinyao Yang, Yongbai Zhang, Jingying Zhang, Horacio Daniel Basigalup, Chunlin Jia

{"title":"Non-coding RNA levels integrating analysis revealed the salt and drought stress response mechanism of miR395e-PvUXS1 pathway in switchgrass.","authors":"Cong Guan, Tingting Wang, Chu Wang, Bo Wu, Zichen Zou, Minli Xu, Xinyao Yang, Yongbai Zhang, Jingying Zhang, Horacio Daniel Basigalup, Chunlin Jia","doi":"10.1016/j.plantsci.2026.113533","DOIUrl":"https://doi.org/10.1016/j.plantsci.2026.113533","url":null,"abstract":"Soil salinization and drought are major constraints on global agricultural production, yet the shared molecular mechanisms by which forage crops respond to these stresses remain incompletedly understood. Identifying candidate genes with potential value for stress-resilient breeding is therefore an important research objective. Switchgrass (Panicum virgatum L.) is a perennial forage and bioenergy crop with adaptation to saline, semi-arid, and drought-prone environments. In this study, RNA sequencing was used to investate the responses of switchgrass to two salt-stress conditions (300mM NaCl treatment and 10‰ soil salinity). Differentially expressed (DE) mRNAs shared by the two salt-stress conditions were significantly enriched in pathways related to carbon metabolism and carbon fixation in photosynthetic organisms. Whole-transcriptome RNA sequencing further identified DE mRNAs, microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) associated with salt and drought responses. Competing endogenous RNA (ceRNA) network analysis identified four KEGG pathways commonly enriched under salt and drought stress: photosynthesis, circadian rhythm in plants, ribosome biogenesis in eukaryotes, and aminoacyl-tRNA biosynthesis. Notably, the UDP-glucuronic acid decarboxylase gene PvUXS1 (Pavir.5NG221800) involved in amino sugar and nucleotide sugar metabolism, was identified as target of miR395e. Salt and drought stress reduced miR395e expression while increasing PvUXS1 expression, and further validation showed that miR395e negatively regulates PvUXS1. Together, these results identify a previously uncharacterized miR395e-PvUXS1 regulatory pathway and provide a potential molecular target for improving stress tolerance in switchgrass.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"113533"},"PeriodicalIF":4.6,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mitochondrial PPR protein AS3 interacts with NPPR2 to facilitate trans-splicing of nad1 intron 1 and ensure kernel development in maize. 玉米线粒体PPR蛋白AS3与NPPR2相互作用促进nad1内含子1的反式剪接并确保籽粒发育

IF 4.6 2区生物学

Plant Science Pub Date : 2026-10-17 DOI: 10.1016/j.plantsci.2026.113516

Kaiji Wang, Qiao Xiao, Binbin Zhao, Jun Yang

{"title":"The mitochondrial PPR protein AS3 interacts with NPPR2 to facilitate trans-splicing of nad1 intron 1 and ensure kernel development in maize.","authors":"Kaiji Wang, Qiao Xiao, Binbin Zhao, Jun Yang","doi":"10.1016/j.plantsci.2026.113516","DOIUrl":"10.1016/j.plantsci.2026.113516","url":null,"abstract":"In higher plants, many mitochondrial genes contain group II introns that undergo intricate splicing processes to produce mature transcripts, which are subsequently translated into functional proteins. However, the identities and specific roles of proteins involved in mitochondrial intron splicing in maize remain largely unclear. In this study, we identified a maize mutant, abnormal seed 3 (as3), through ethyl methanesulfonate (EMS) mutagenesis and mapped the causal gene using bulked segregant analysis. AS3 encodes a mitochondrion-localized P-subgroup pentatricopeptide repeat (PPR) protein containing 21 PPR motifs. Mutation of AS3 substantially reduces the trans-splicing of mitochondrial nad1 intron 1, resulting in the accumulation of nad1 exon 1 precursors and a pronounced reduction in mature nad1 transcripts. These splicing defects compromise the assembly and activity of respiratory complex I, alter mitochondrial morphology, and activate the alternative oxidase pathway, as evidenced by elevated transcript levels of alternative oxidases. Furthermore, AS3 physically interacts with the PPR protein NPPR2, whose loss of function similarly disrupts nad1 intron 1 splicing and further decreases nad1 transcript and protein abundance. Collectively, these results demonstrate that AS3 and NPPR2 physically interact and act cooperatively in the trans-splicing of nad1 intron 1, a process essential for mitochondrial function and proper kernel development in maize.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"113516"},"PeriodicalIF":4.6,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative spatiotemporal regulation of iron and zinc transporters in developing tissues of temperate rice genotypes. 温带水稻基因型发育组织中铁和锌转运体的时空调控比较

IF 4.6 2区生物学

Plant Science Pub Date : 2026-10-17 DOI: 10.1016/j.plantsci.2026.113520

Anjali Verma, Adfar Amin, Jebi Sudan, Najeeb-Ul-Rehman Sofi, Parvaze A Sofi, Sajad Majeed Zargar

{"title":"Comparative spatiotemporal regulation of iron and zinc transporters in developing tissues of temperate rice genotypes.","authors":"Anjali Verma, Adfar Amin, Jebi Sudan, Najeeb-Ul-Rehman Sofi, Parvaze A Sofi, Sajad Majeed Zargar","doi":"10.1016/j.plantsci.2026.113520","DOIUrl":"10.1016/j.plantsci.2026.113520","url":null,"abstract":"Hidden hunger (micronutrient deficiency) contributes majorly for poor mental and physical health for every age group and gender across the world. Here we integrated rice grain ionome with high resolution tissue and developmental stage specific transcriptomic to elucidate the molecular basis of Fe/Zn accumulation in two contrasting temperate rice genotypes: the high mineral pigmented genotype Zag-V9 (20.53 ppm Fe and 53.41 ppm Zn in its husked grains) and the low-mineral white-grained genotype GS-74 (13.40 ppm Fe and 23.71 ppm Zn in its husked grains). Among 498 identified transporter gene, major Fe/Zn transporter families, including ZIP/IRT, YSL, NRAMP, HMA, NAS, VIT, ZIFL, TOM/NAAT and ABC transporters, displayed significantly stronger induction in Zag-V9, particularly during reproductive satge. During grain filling, 195 transporter genes (69.1%) were upregulated in Zag-V9 compared with only 58 (31%) in GS-74, whereas GS-74 showed predominant transcriptional repression (129 genes; 69%). Integrated regulatory network analysis further revealed enhanced activation of bHLH, WRKY, NAC, MYB, ERF and bZIP transcription factors together with enrichment of pathways associated with metal homeostasis, photosynthesis, carbon metabolism, redox regulation and energy-dependent transport. This work provides one of the first comprehensive spatiotemporal regulatory frameworks integrating ionomic validation, developmental transcriptomics and transporter network biology in temperate rice and identifies key candidate genes-including OsYSL2, OsYSL6, OsZIP6, OsNRAMP5, OsNAS1 and HMA3 family transporters-together with their upstream transcriptional regulators as promising targets for genomics-assisted breeding, genome editing and next-generation biofortification strategies to combat global hidden hunger.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"113520"},"PeriodicalIF":4.6,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The bZIP transcription factor ZmbZIP111 contributes to drought tolerance and regulates the expression of ZmEULD1b in maize. bZIP转录因子ZmbZIP111参与玉米抗旱性调控ZmEULD1b的表达。

IF 4.6 2区生物学

Plant Science Pub Date : 2026-10-17 DOI: 10.1016/j.plantsci.2026.113532

Haidong Yan, Dongxu Zhao, Chen Liu, Xueru Zhu, Xueshi Zhang, Zhongxian Ma, Qing Ma, Ronghao Cai

{"title":"The bZIP transcription factor ZmbZIP111 contributes to drought tolerance and regulates the expression of ZmEULD1b in maize.","authors":"Haidong Yan, Dongxu Zhao, Chen Liu, Xueru Zhu, Xueshi Zhang, Zhongxian Ma, Qing Ma, Ronghao Cai","doi":"10.1016/j.plantsci.2026.113532","DOIUrl":"https://doi.org/10.1016/j.plantsci.2026.113532","url":null,"abstract":"Drought stress severely constrains maize productivity, and elucidating the molecular mechanisms underlying drought tolerance is crucial for developing drought-tolerant cultivars. In this study, we characterized the function of the drought-inducible bZIP transcription factor ZmbZIP111 and found that the bzip111 mutant exhibited increased sensitivity to drought stress. Compared with the wild type, the bzip111 mutants exhibited more pronounced inhibition of root growth under water-deficit conditions, accompanied by significantly reduced survival rates and relative water content. Physiological assays further demonstrated that the mutant accumulated higher levels of malondialdehyde and exhibited decreased superoxide dismutase activity. To elucidate the underlying molecular mechanism, we performed transcriptome analysis and identified ZmEULD1b, a gene previously reported to positively regulate root development and drought tolerance in maize, as a candidate downstream target of ZmbZIP111. Notably, the transcript level of ZmEULD1b was significantly reduced in the bzip111 mutant compared with that in the wild type. Collectively, our findings suggest that ZmbZIP111 contributes to drought tolerance in maize and is associated with the regulation of ZmEULD1b expression. This study provides a valuable candidate gene for the genetic improvement of drought tolerance in maize.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"113532"},"PeriodicalIF":4.6,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide characterization of Ferritin gene family reveals BnaFER1.1 as a key regulator of salt stress tolerance in rapeseed (Brassica napus). 铁蛋白基因家族的全基因组特征揭示了BnaFER1.1是油菜耐盐性的关键调控因子。

IF 4.6 2区生物学

Plant Science Pub Date : 2026-10-16 DOI: 10.1016/j.plantsci.2026.113528

Zhihao Jia, Dazhuang Qi, Zihan Xue, Mei Wang, Dong Li, Yanfeng Liu, Hongxia Zhang, Nan Sun, Limin Wang

{"title":"Genome-wide characterization of Ferritin gene family reveals BnaFER1.1 as a key regulator of salt stress tolerance in rapeseed (Brassica napus).","authors":"Zhihao Jia, Dazhuang Qi, Zihan Xue, Mei Wang, Dong Li, Yanfeng Liu, Hongxia Zhang, Nan Sun, Limin Wang","doi":"10.1016/j.plantsci.2026.113528","DOIUrl":"10.1016/j.plantsci.2026.113528","url":null,"abstract":"Ferritins (FERs) are essential iron-storage proteins that maintain iron homeostasis, yet their contributions to growth and salt tolerance in Brassica napus remain poorly understood. In this study, a genome-wide analysis identified 9 BnaFER genes in the rapeseed genome. These genes were unevenly distributed across eight chromosomes and exhibited conserved gene structures and protein domains, indicating evolutionary conservation. Promoter analysis revealed numerous cis-acting elements associated with hormone, light, and stress responses. Expression profiling revealed that most BnaFER genes responded to salt stress, with both induction and repression patterns under NaCl treatment. BnaFER1.1, a chloroplast-localized member, was selected for functional characterization due to its strong stress responsiveness. Heterologous expression in Arabidopsis enhanced plant growth, increased silique length, silique number, and seed area and significantly improved salt tolerance. Physiological analyses revealed increased activities of catalase (CAT) and peroxidase (POD) and reduced accumulation of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA). Moreover, AtNHX1 and AtRD29A were significantly upregulated under salt stress in transgenic plants, indicating potential for improved ion homeostasis and osmotic adjustment. Collectively, BnaFER1.1 enhances salt tolerance through coordinated regulation of ROS detoxification and stress-responsive pathways. Our findings provide an important genetic foundation for the functional elucidation of BnaFER1.1 genes and a novel gene resource for improving salt tolerance in rapeseed.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"113528"},"PeriodicalIF":4.6,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating physiology, transcriptomics and phylogenomics reveals the adaptive evolution of biennial fruiting in angiosperms. 整合生理学，转录组学和系统基因组学揭示了被子植物二年生结果的适应性进化。

IF 4.6 2区生物学

Plant Science Pub Date : 2026-10-16 DOI: 10.1016/j.plantsci.2026.113529

Gui-Liang Xin, Yu-Ting Li, Gang Wang, Bo-Bin Liu

{"title":"Integrating physiology, transcriptomics and phylogenomics reveals the adaptive evolution of biennial fruiting in angiosperms.","authors":"Gui-Liang Xin, Yu-Ting Li, Gang Wang, Bo-Bin Liu","doi":"10.1016/j.plantsci.2026.113529","DOIUrl":"10.1016/j.plantsci.2026.113529","url":null,"abstract":"Most angiosperms complete fruit maturation within a single growing season, but a few lineages require two. However, the physiological and molecular mechanisms underlying this prolonged fruit development (PFD), and whether it represents a shared ancestral trait or a convergent adaptation, remain unresolved. Here we investigate 2-year fruiting species in Fagaceae, Rhamnaceae and Tapisciaceae, using morphological, histochemical, transcriptomic and phylogenomic approaches. The results show that fruits enter dormancy in autumn, resume growth in spring, and accumulate sugars and lipids during overwintering, indicating cold acclimation via starch/sucrose and fatty acid metabolism. Transcriptomic and co‑expression network analyses reveal that phytohormone (ABA, ethylene, auxin) and MAPK signaling are intertwined with sugar metabolism, forming a regulatory hub that controls dormancy. Crucially, by integrating phylogenomic dating across multiple lineages, we demonstrate that PFD did not arise from a common ancestor but evolved convergently, with the divergence of major PFD-bearing clades (Fagaceae, Rhamnaceae, Tapisciaceae) clustering near the Eocene-Oligocene boundary-a period of profound global cooling. Our findings provide a mechanistic and evolutionary framework for understanding PFD as a convergent adaptive strategy, linking molecular regulation of winter dormancy to deep-time climatic change.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"113529"},"PeriodicalIF":4.6,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-limitation mechanism governs ethylamine supply for theanine biosynthesis in tea plants. 茶树茶氨酸生物合成中乙胺的供应受双重限制机制支配。

IF 4.6 2区生物学

Plant Science Pub Date : 2026-10-15 DOI: 10.1016/j.plantsci.2026.113527

Xiaolu Wang, Ping Li

{"title":"A dual-limitation mechanism governs ethylamine supply for theanine biosynthesis in tea plants.","authors":"Xiaolu Wang, Ping Li","doi":"10.1016/j.plantsci.2026.113527","DOIUrl":"https://doi.org/10.1016/j.plantsci.2026.113527","url":null,"abstract":"L-Theanine is a characteristic non-proteinogenic amino acid in tea plants (Camellia sinensis) that determines tea quality. Its biosynthesis depends on ethylamine supply, but the specific source of ethylamine and the fine regulatory mechanisms remain unclear. In this study, we integrated short-term low-sugar treatments (stLS), virus-induced gene silencing (VIGS), transient expression in tobacco, genetic transformation of tea callus, and subcellular localization analyses to investigate the regulatory roles of alanine supply, alanine decarboxylase (CsAlaDC), and S-adenosylmethionine decarboxylase (CsSAMDC). stLS treatment significantly reduced alanine levels in callus and caused concomitant decreases in ethylamine and theanine, confirming that alanine supply is a key upstream limiting factor. Notably, VIGS-mediated silencing of alanine aminotransferase (CsALT) caused fluctuations in alanine content, suggesting complex metabolic compensation. Heterologous expression in tobacco demonstrated that both CsAlaDC and CsSAMDC could supply ethylamine for CsTS to promote theanine accumulation, whereas serine decarboxylase (CsSDC) did not. In tea callus, silencing CsAlaDC drastically reduced ethylamine and impaired theanine biosynthesis, while its overexpression promoted ethylamine accumulation but did not trigger substantial theanine production. In other words, the bottleneck shifts to 'downstream limitations'. In contrast, CsSAMDC silencing showed no significant difference from the wild type. Subcellular localization showed that CsTS was predominantly distributed in the cytoplasm. Collectively, this study proposes a dual-limitation model for theanine biosynthesis, revealing a coordinated regulatory mechanism involving precursor supply and metabolic pathway activity.","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"113527"},"PeriodicalIF":4.6,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149195984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0