Plant Science最新文献

{"title":"Genomic insights into assembly of α-β hydrolase superfamily genes involved in blast resistance in rice","authors":"Qianmei Zou ,&nbsp;Longyuan Zhao ,&nbsp;Lirong Guan ,&nbsp;Ninghui Pan ,&nbsp;Ping He ,&nbsp;Guangyu Han ,&nbsp;Yunlong Du ,&nbsp;Yong Xie","doi":"10.1016/j.plantsci.2025.112605","DOIUrl":"10.1016/j.plantsci.2025.112605","url":null,"abstract":"<div><div>The α-β hydrolase (ABH) gene encodes growth, development, and stress-associated enzymes in various plants; however, its evolution and function in cereal crops remain largely unexplored. In this study, bioinformatics analysis was performed to define and predict <em>OsABH</em> superfamily evolution and function in <em>Oryza indica</em>. Then transgenic rice plants overexpressing the <em>OsABH</em> superfamily gene <em>BGIOSGA016507</em> were generated, and the expression dynamics of disease resistance factors in response to <em>Magnaporthe oryzae</em> infection were assessed using RT-qPCR. Meanwhile, endogenous hormones levels were quantitatively determined through ELISA. Results indicated that 41 <em>ABHs</em> were identified in <em>O. indica</em>. Interspecies synteny analysis revealed that <em>OsABH</em> shared the highest number of syntenic gene pairs with <em>TaABH</em> (86 pairs), compared to only 10 pairs with <em>AtABH</em>, suggesting the closest evolutionary relationship between the <em>OsABH</em> superfamily in <em>O. indica</em> and <em>Triticum aestivum</em>. These findings also indicated that the <em>OsABH</em> superfamily amplification is linked to segmental duplications. Besides, the <em>OsABH</em> promoter exhibited multiple biotic and abiotic stress and tolerance-associated cis-acting elements. <em>BGIOSGA016507</em> haplotypes analysis showed an obvious indica bias. Additionally, <em>BGIOSGA016507</em> responded to rice blast stress. Compared to wild-type Nipponbare, the OE-016507 showed significantly smaller lesion areas post <em>M. oryzae</em> inoculation (<em>P</em> &lt; 0.001). Concurrently, significant differences were observed in <em>PRs</em> expression and endogenous hormones levels, with salicylic acid reaching a peak concentration of 228.75 pmol/L at 36 hpi, approximately twice the level detected in NPB. This study sheds light on the understanding of <em>ABH</em> superfamily evolution in rice and validates <em>OsABH</em> involvement in blast-resistant response.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112605"},"PeriodicalIF":4.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Alkalinization Factor – A cryptide regulating developmental and stress responses","authors":"Durvasula Sumana Pratyusha,&nbsp;Dronamraju V.L. Sarada","doi":"10.1016/j.plantsci.2025.112600","DOIUrl":"10.1016/j.plantsci.2025.112600","url":null,"abstract":"<div><div>Post-translational modification is a key component that aids in regulated signaling cascades and complex cross talks in the plant cell to eventually facilitate efficient plant growth and development during the transition phases and in response to environment hindrances. Proteolytic processing, being one of the irreversible post-translational modifications, resulting in generation of mature peptide hormones from large precursor proteins, enhance the rapid responses, cell – cell communication including regulation of hormone signaling, basic cellular functions and defense activities. Often most of the proteolytic products are bio-active in nature and such peptides comprises the cryptome of the plant cell. The probability of generation of cryptides would be more via the unfolded or unstructured regions known as Intrinsic disordered regions which has higher sensitivity towards the proteases when compared to the structured regions. Similar to CLE, systemin, PSK (phytosulfokine) and RGF (Root growth factor), RALF, a small secreted cryptic peptide regulates the developmental and stress responses upon proteolytic cleavage. Apart from the identification and <em>in-silico</em> analysis of RALF peptides in different plant species, the role of these peptides in molecular signaling remains largely unknown. This review focuses on the perspective of RALF as a cryptic peptide explains the known roles of the peptide in developmental and stress responses. Further the roles of RALF in terms of molecular signaling are speculated to provide insights into complex growth, development and stress responses.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112600"},"PeriodicalIF":4.2,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aquaporin GhPIP2;1 positively regulates salt tolerance in upland cotton (Gossypium hirsutum L.)","authors":"Yiran Li ,&nbsp;Boying Lian ,&nbsp;Yanna Gao ,&nbsp;Nan Zhang ,&nbsp;Adeel Ahmad ,&nbsp;Mengyan Wang ,&nbsp;Hongmei Wu ,&nbsp;Jiajia Wu ,&nbsp;Fan Yang ,&nbsp;Baoquan Wang ,&nbsp;Fushun Hao ,&nbsp;Hengling Wei","doi":"10.1016/j.plantsci.2025.112601","DOIUrl":"10.1016/j.plantsci.2025.112601","url":null,"abstract":"<div><div>Aquaporins (AQPs) facilitate transmembrane water transport and play a crucial role in plant adaptation to diverse abiotic stress conditions. Plasma membrane Intrinsic Proteins (PIPs) are a crucial subgroup of aquaporins that facilitate water transport across plant cell membrane. However, the role of PIPs under salt stress responses in cotton remains partially unexplored. In this study, we found that the expression level of the aquaporin gene <em>GhPIP2;1</em> was significantly upregulated under salt stress at three-leaf stage in upland cotton. The overexpression of <em>GhPIP2;1</em> in <em>Arabidopsis thaliana</em> and virus-induced gene silencing (VIGS) in upland cotton evidenced that <em>GhPIP2;1</em> positively regulate salt tolerance function. Biochemical analysis including superoxide dismutase (SOD), peroxidase (POD) activities and malondialdehyde (MDA) content measurements indicated that <em>GhPIP2;1</em> could enhance stress tolerance by regulating the reactive oxygen species (ROS). The Electrophoretic mobility shift assay (EMSA) and dual luciferase reporter assay confirmed that GhNAC072 specifically binds to the promoter region of <em>GhPIP2;1</em> and enhance the expression of <em>GhPIP2;1</em>. Phenotypic observations and physiological measurements of <em>GhNAC072</em> overexpressed <em>Arabidopsis</em> and silenced cotton plants demonstrated that <em>GhNAC072</em> positively responds to salt stress. The expression level of <em>GhPIP2;1</em> was significantly decreased in <em>GhNAC072</em>-silenced plants, indicating that <em>GhNAC072</em> enhances salt tolerance by regulating <em>GhPIP2;1</em> expression. In summary, this study systematically confirmed that <em>GhPIP2;1</em> may involve in salt tolerance mechanism in cotton and its expression is regulated by <em>GhNAC072</em>. These results will provide gene resources for salt tolerance and enrich the knowledge on the mechanism of salt tolerance in cotton.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112601"},"PeriodicalIF":4.2,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiation hormesis and reactive oxygen species-mediated stress priming in plants","authors":"Jin-Hong Kim","doi":"10.1016/j.plantsci.2025.112602","DOIUrl":"10.1016/j.plantsci.2025.112602","url":null,"abstract":"<div><div>Radiation hormesis refers to the beneficial biological effects of low-dose ionizing radiation (LDIR) that do not conform to linear-no-threshold or threshold models. Ionizing radiation has been extensively used in the bioindustry for mutation induction, food sterilization, and structural modification of natural compounds. Despite its industrial potential, the application of LDIR-induced hormetic effects remains limited owing to insufficient scientific evidence, reproducibility concerns, and policy considerations related to radiation safety management. This review examines representative cases of LDIR-induced hormetic effects in plants, along with key factors and mechanisms, to explore their industrial applicability. The hormetic effects of LDIR were demonstrated in terms of yield, fitness, and versatility. Since radiation hormesis is determined by the interplay among key factors including radiation, organisms, and the environment, their significance and contributions are highlighted. Moreover, reactive oxygen species-mediated stress priming and memory facilitated by chromatin dynamics and epigenetic regulation have been proposed as key mechanisms of radiation hormesis. Finally, the potential applications of LDIR-induced and ROS-mediated stress priming in the bioindustry are discussed.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112602"},"PeriodicalIF":4.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of the RALF gene family in Camellia oleifera Abel. and the potentiality of CoRALF50 in pollen tube growth","authors":"Mengqi Lu ,&nbsp;Junqin Zhou ,&nbsp;Yiyang Gu ,&nbsp;Yanling Zeng ,&nbsp;Kaizheng Lu ,&nbsp;Shaofeng Peng ,&nbsp;Xiaofeng Tan","doi":"10.1016/j.plantsci.2025.112603","DOIUrl":"10.1016/j.plantsci.2025.112603","url":null,"abstract":"<div><div>Rapid alkalinization factors (RALFs) play meaningful roles in the pollination and fertilization. However, the identification and study of Camellia RALFs is lacking, particularly the function of the RALF family in regulating self-incompatibility pollen tube growth. Herein, we identified 50 <em>RALF</em> genes from <em>Camellia oleifera</em> genome, and classified them into three groups: Clades Ⅰ, Ⅱ, and Ⅲ, with 11, 12, and 27 members being included, respectively. <em>CoRALFs</em> were unevenly distributed in chromosomes, and the segmental duplication events mainly facilitated their expansion. Gene structure and conserved motif analyses indicated that they were highly conserved. Cis-element analysis revealed that many light responsive elements, stress responsive elements and phytohormone responsive elements were found in <em>CoRALFs</em> promoters. Moreover, the expression analysis showed that pollen-specific <em>CoRALF50</em> responded to the elongation and stagnation of self-incompatibility pollen tube in <em>C. oleifera</em>. Further experiments suggested that <em>CoRALF50</em> could significantly down-regulate carbohydrate metabolism pathways in pollen tubes cultured <em>in vitro</em>, thereby causing abnormalities in ROS level, plasma membrane biosynthesis, and cell wall biosynthesis, which demonstrating the importance of CoRALF50 in regulating pollen tube growth. Altogether, this study extends information for exploring new functions of RALFs and provides new insights into the role of RALFs in self-incompatibility pollen tube growth.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112603"},"PeriodicalIF":4.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The MYB transcription factor ClPC modulates petal color and chlorophyll accumulation in watermelon","authors":"Dongming Liu ,&nbsp;Liming Zhang ,&nbsp;Aofeng Hu ,&nbsp;Dongling Sun ,&nbsp;Yaxin Chen ,&nbsp;Shixiang Duan ,&nbsp;Yachen Liu ,&nbsp;Junling Dou ,&nbsp;Sen Yang ,&nbsp;Huanhuan Niu ,&nbsp;Wenkai Yan ,&nbsp;Jianbin Hu ,&nbsp;Huayu Zhu ,&nbsp;Luming Yang","doi":"10.1016/j.plantsci.2025.112586","DOIUrl":"10.1016/j.plantsci.2025.112586","url":null,"abstract":"<div><div>During the pre-anthesis stage, high chlorophyll levels in petals result in a green hue in many plants. Upon anthesis, chlorophyll degradation uncovers other pigments, thereby influencing pollinator attraction and reproductive success in insect-pollinated crops. In the watermelon accession WM109, a novel petal phenotype characterized by a yellow-green hue was observed, significantly different from the canonical yellow petal phenotype typically associated with this species. Genetic analysis using F₂ populations revealed that this yellow-green petal trait is controlled by a single recessive gene. By screening SSR primer pairs with the constructed yellow and yellow-green DNA pools and genotyping F₂ individuals, the responsible gene was mapped to a 139.7 kb interval on watermelon chromosome 11, containing two candidate genes. Through sequence analysis, expression profiling, and functional verification of these candidates, a gene encoding a MYB transcription factor with a base insertion was identified as the key determinant of this unusual phenotype, which is characterized by elevated chlorophyll levels and increased chloroplast density. Given the limited current knowledge regarding the relationship between MYB transcription factors and chlorophyll biosynthesis, these findings enhance our understanding of the molecular mechanisms underlying chlorophyll production.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112586"},"PeriodicalIF":4.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root architecture and the rhizosphere microbiome: Shaping sustainable agriculture","authors":"Ademir S.F. Araujo ,&nbsp;Arthur P.A. Pereira ,&nbsp;Erika V. de Medeiros ,&nbsp;Lucas W. Mendes","doi":"10.1016/j.plantsci.2025.112599","DOIUrl":"10.1016/j.plantsci.2025.112599","url":null,"abstract":"<div><div>Understanding root architecture and exudation is fundamental for enhancing crop productivity and promoting sustainable agriculture. Historically, plant researchers have focused on above-ground traits to increase yield and reduce input dependence. However, below-ground traits, especially those related to the root system, are equally critical yet often overlooked due to phenotyping challenges. Root architecture, including some root traits, i.e., lateral root density, root hair abundance, and root tip number, plays central roles in plant establishment, stress tolerance, and the recruitment of beneficial microbes in the rhizosphere. Root exudates, a complex array of chemical compounds released by roots, vary with plant species, developmental stage, and environmental conditions. These compounds act as signals and nutrients, shaping the composition and function of rhizosphere microbial communities. In turn, the microbiome of rhizosphere contributes to plant health by facilitating nutrient uptake, enhancing stress resilience, and providing defense against pathogens. Integrating root traits into breeding programs offers promising opportunities to select for genotypes that are more efficient in recruiting beneficial microbes. Heritable root traits, such as increased branching, finer roots, and higher exudation capacity, can enhance microbiome assembly and stability. The assessment of genes can also regulate of these traits and represent targets for genomics-assisted selection. Some strategies, such as microbiome engineering, particularly through the design of synthetic microbial communities (SynComs), can be used to modulate root architecture and optimize plant-microbe interactions. Despite these promising outcomes, challenges remain in translating SynCom applications to the field due to environmental variability, native microbial competition, and limited understanding of host genetic controls. This review discusses how root architecture shapes the rhizosphere microbiome and explores strategies, such as trait-based breeding and microbiome engineering, for advancing sustainable crop production.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112599"},"PeriodicalIF":4.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress memory increases cane yield and juice quality of field-grown sugarcane","authors":"Maria D. Pissolato ,&nbsp;Larissa P. Cruz ,&nbsp;Rafael L. Almeida ,&nbsp;Tamires S. Martins ,&nbsp;Eduardo C. Machado ,&nbsp;Júlio C. Garcia ,&nbsp;Marcos G.A. Landell ,&nbsp;Mauro A. Xavier ,&nbsp;Rafael V. Ribeiro","doi":"10.1016/j.plantsci.2025.112598","DOIUrl":"10.1016/j.plantsci.2025.112598","url":null,"abstract":"<div><div>Intergenerational drought memory has been studied in several sexually reproducing plant species, but the occurrence of this phenomenon and the potential effects on yield of clonal plants are unknown. We investigated the effects of intergenerational drought memory on productivity of sugarcane propagules obtained from plants previously stressed at two phenological stages. Firstly, IACCTC07–8008 (drought tolerant) and IACSP95–5000 (high-yielding) cultivars were grown under well-hydrated conditions (group W) or subjected to three cycles of water deficit through water withholding during the tillering (group T) or maturation (group M) stage. Then, propagules from these three groups were grown under field conditions. Propagules from groups T and M of IACCTC07–8008 showed enhanced photosynthesis, attributed to increased stomatal conductance, photochemical activity, Rubisco carboxylation, and a larger root system. Group M of IACCTC07–8008 exhibited reduced ascorbate peroxidase activity, leading to increased H₂O₂ concentration without causing oxidative damage. This group also showed improved juice quality – such a higher sucrose content. The stalk yield, shoot biomass, leaf area index and tiller density of the group M of IACCTC07–8008 and group T of IACSP95–5000 were increased as a consequence of intergenerational drought memory. Although both cultivars have “inherited” drought memory, our findings suggest that parental stress memory varies between cultivars and phenological stages in sugarcane. This research is the first to demonstrate the impact of intergenerational drought memory on yield and juice quality in field-grown sugarcane, providing valuable insights for enhancing drought tolerance strategies in sugarcane production, with significant potential benefits for the industry.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112598"},"PeriodicalIF":4.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 13-bp insertion in CmAPRR2 gene disrupts its function in regulating the green rind formation of immature melon fruit (Cucumis melo L.)","authors":"Liqin Chen ,&nbsp;Jing Dong ,&nbsp;Zongqing Qiu ,&nbsp;Runhua Bu ,&nbsp;Yu Zhou ,&nbsp;Yuhong Li ,&nbsp;Huilin Wang ,&nbsp;Liangliang Hu","doi":"10.1016/j.plantsci.2025.112590","DOIUrl":"10.1016/j.plantsci.2025.112590","url":null,"abstract":"<div><div>Melon (<em>Cucumis melo</em> L.) fruit rind color represents a crucial agronomic trait that significantly influences consumer preference, market value, and postharvest quality. Although economically important, the molecular mechanisms regulating rind color variation remain largely unexplored. In this study, we identified and characterized a key gene controlling the immature fruit rind color from two melon inbred lines, TC3 with immature green fruit rind and Tm34 with immature light-green fruit rind. Genetic segregation analysis across multiple populations demonstrated that immature green fruit rind color is governed by a single completely dominant gene, designated as <em>GR</em>. Fine mapping strategies delimited the <em>GR</em> locus to a 27.14 kb region on chromosome 4 containing five candidate genes. Sequence analysis revealed a 13-bp insertion specifically within the seventh exon of <em>CmAPRR2</em>, encoding a two-component response regulator-like protein APRR2. The insertion introduced a premature stop codon, leading to a truncated protein. Allelic diversity assessment among 40 natural melon accessions revealed a strong correlation between rind color phenotype and the <em>CmAPRR2</em> insertion polymorphism. Expression analysis demonstrated significantly higher <em>CmAPRR2</em> transcript levels in green rinds compared to light-green rinds during immature fruit development. Consistent with this observation, genes involved in chlorophyll biosynthesis and chloroplast development were also upregulated in green rinds. While the insertion mutation did not affect the nuclear localization of <em>CmAPRR2</em>, it exhibited a significant reduction in its transcriptional activation capacity. Further, employing virus-induced gene silencing (VIGS) to silence the <em>SlAPRR2</em> gene in tomato led to the development of light-green fruit coloration and a marked downregulation of <em>APRR2</em> gene expression. These findings provide novel insights into the molecular mechanisms underlying melon rind color formation and identify <em>CmAPRR2</em> as a promising genetic resource for molecular breeding programs aimed at manipulating rind color traits in melon.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112590"},"PeriodicalIF":4.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144222070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of Cordyceps militaris Cmcns1/cns2 gene to produce cordycepin results in increased resistance to Ralstonia solawacearum, Alternaria alternate and TMV in tobacco(Nicotiana tabacum L.)","authors":"Jie Tan ,&nbsp;Tao Long ,&nbsp;Juntao Song ,&nbsp;Tianxiunan Pu ,&nbsp;Yuanshuai Shi ,&nbsp;Xu Luo ,&nbsp;Yang Liu","doi":"10.1016/j.plantsci.2025.112597","DOIUrl":"10.1016/j.plantsci.2025.112597","url":null,"abstract":"<div><div>Cordycepin (COR), the first nucleoside antibiotic isolated from fungi, exhibits antibacterial, antitumor, antiviral, and anti-inflammatory activities. In this study, we constructed a <em>Cmcns1/cns2</em> fusion gene vector and transformed tobacco using <em>Agrobacterium tumefaciens</em>-mediated genetic transformation. The results showed that cordycepin was detected in transgenic tobacco plants, with <em>Cmcns1/cns2</em> expression confirmed and the highest expression level observed in line OE1. This suggests a direct correlation between COR production and <em>Cmcns1/cns2</em> gene expression levels. Importantly, following infection with viral or bacterial pathogens, the <em>Cmcns1/cns2</em>-overexpressing tobacco plants exhibited fewer wilting lesions compared to wild-type plants, indicating that <em>Cmcns1/cns2</em> expression contributes to COR production, thereby enhancing disease resistance. We measured malondialdehyde (MDA) content, as well as superoxide dismutase (SOD) and peroxidase (POD) activity, before and after treatment. The results demonstrated that MDA content in transgenic tobacco was lower than pre-treatment levels, whereas SOD and POD activities were higher than pre-treatment values. Furthermore, expression levels of disease resistance genes <em>NtADR1</em>, <em>NtNPR1</em>, and <em>NtNRG1</em> in transgenic line OE1 were significantly elevated compared to wild-type plants following treatment with <em>Ralstonia solanacearum</em> and <em>Alternaria alternata</em>. This confirms that the transgenic <em>Cmcns1/cns2</em> gene enhances disease resistance in tobacco, presenting a new approach for breeding plants with improved disease resistance.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"359 ","pages":"Article 112597"},"PeriodicalIF":4.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}