Plant Science最新文献_第2页

Comprehensive insights on association mapping in perennial fruit crops breeding – Its implications, current status and future perspectives 关于多年生水果作物育种中关联图谱的全面见解--其意义、现状和未来展望。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-18 DOI: 10.1016/j.plantsci.2024.112281

Ashwini Zadokar , Parul Sharma , Rajnish Sharma

{"title":"Comprehensive insights on association mapping in perennial fruit crops breeding – Its implications, current status and future perspectives","authors":"Ashwini Zadokar , Parul Sharma , Rajnish Sharma","doi":"10.1016/j.plantsci.2024.112281","DOIUrl":"10.1016/j.plantsci.2024.112281","url":null,"abstract":"<div><div>In order to provide food and nutritional security for the world's rapidly expanding population, fruit crop researchers have identified two critical priorities: increasing production and preserving fruit quality during the pre- and post-harvest periods. The genetic basis of these complex, commercially important fruit traits which are uniquely regulated by polygenes or multi-allelic genes that interact with one another and the environment can be analyzed with the aid of trait mapping tools. The most interesting trait mapping approach that offers the genetic level investigation for marker-trait associations (MTAs) for these complex fruit traits, without the development of mapping population, is association mapping. This approach was used during the genetic improvement program, emphasizing the obstacles (breeding strategies adopted, generation interval, and their genomic status) pertaining to perennial fruit crops. This method of studying population diversity and linkage disequilibrium in perennial fruit crops has been made possible by recent developments in genotyping, phenotyping, and statistical analysis. Thus, the purpose of this review is to provide an overview of different trait mapping techniques, with a focus on association mapping (method, essential components, viability, constraints, and future perspective) and its advantages, disadvantages, and possibilities for breeding perennial fruit crops.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of efficient and scalable regeneration tissue culture method for Cannabis sativa 开发高效、可扩展的大麻再生组织培养方法。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-18 DOI: 10.1016/j.plantsci.2024.112296

Aleksei Sorokin, Igor Kovalchuk

{"title":"Development of efficient and scalable regeneration tissue culture method for Cannabis sativa","authors":"Aleksei Sorokin, Igor Kovalchuk","doi":"10.1016/j.plantsci.2024.112296","DOIUrl":"10.1016/j.plantsci.2024.112296","url":null,"abstract":"<div><div>Large scale production of uniform disease-free plants is crucial for <em>Cannabis sativa</em> biotechnology. Existing micropropagation protocols rely heavily on shoot multiplication from existing meristems via direct organogenesis. Such protocols do not allow multiplication of plant material through continuous sub-culturing. Protocols that use indirect regeneration are usually not efficient enough and have very low multiplication rates. In the present study, an efficient protocol that uses a combination of direct organogenesis and callogenesis to induce multiple shoot development cultures is developed. Callogenesis was induced from various explants cultured on the media having various combinations of thidiazuron (TDZ) and naphthaleneacetic acid (NAA); best callogenesis and shoot regeneration was achieved from hypocotyl explants cultured on TDZ 0.4 mg l<sup>−1</sup> NAA 0.2 mg l<sup>−1</sup>. Hypocotyls with cotyledonary node and shoot apical meristem were significantly better for shoot regeneration than explants without it. Shoots obtained from multiple shoot cultures were successfully rooted and then acclimatized under greenhouse conditions to develop into adult cannabis plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measuring CO2 assimilation of Arabidopsis thaliana whole plants and seedlings 测量拟南芥全株和幼苗的二氧化碳同化能力

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-17 DOI: 10.1016/j.plantsci.2024.112295

Ailbhe J. Brazel , Niranjana S. Manoj , Franziska Turck , Diarmuid S. Ó’Maoiléidigh

{"title":"Measuring CO2 assimilation of Arabidopsis thaliana whole plants and seedlings","authors":"Ailbhe J. Brazel , Niranjana S. Manoj , Franziska Turck , Diarmuid S. Ó’Maoiléidigh","doi":"10.1016/j.plantsci.2024.112295","DOIUrl":"10.1016/j.plantsci.2024.112295","url":null,"abstract":"<div><div>Photosynthesis is an essential process in plants that synthesizes sugars used for growth and development, highlighting the importance of establishing robust methods to monitor photosynthetic activity. Infrared gas analysis (IRGA) can be used to track photosynthetic rates by measuring plant CO<sub>2</sub> assimilation and release. Although much progress has been made in the development of IRGA technologies, challenges remain when using this technique on small herbaceous plants such as <em>Arabidopsis thaliana</em>. The use of whole plant chambers can overcome the difficulties associated with applying bulky leaf clamps to small delicate leaves. However, respiration from the roots and from soil-based microorganisms may skew these gas exchange measurements. Here, we present a simple method to efficiently perform IRGA on <em>A. thaliana</em> plants using a whole plant chamber that removes the confounding effects of respiration from roots and soil-based microorganisms from the measurements. We show that this method can be used to detect subtle changes in photosynthetic rates measured at different times of day, under different growth conditions, and between wild-type and plants with deficiencies in the photosynthetic machinery. Furthermore, we show that this method can be used to detect changes in photosynthetic rates even at very young developmental stages such as 10 d-old seedlings. This method contributes to the array of techniques currently used to perform IRGA on <em>A. thaliana</em> and can allow for the monitoring of photosynthetic rates of whole plants from young ages.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mediator subunit 17 regulates light and darkness responses in Arabidopsis plants 介体亚基 17 调节拟南芥植物的光照和黑暗反应。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-16 DOI: 10.1016/j.plantsci.2024.112285

Marisol Giustozzi , Santiago Nicolás Freytes , María Lorena Falcone Ferreyra , Pablo Cerdán , Paula Casati

{"title":"Mediator subunit 17 regulates light and darkness responses in Arabidopsis plants","authors":"Marisol Giustozzi , Santiago Nicolás Freytes , María Lorena Falcone Ferreyra , Pablo Cerdán , Paula Casati","doi":"10.1016/j.plantsci.2024.112285","DOIUrl":"10.1016/j.plantsci.2024.112285","url":null,"abstract":"<div><div>Mediator 17 (MED17) is part of the head of the Mediator complex, which regulates transcription initiation in different eukaryotic organisms, including plants. We have previously characterized MED17 roles in Arabidopsis plants exposed to UV-B radiation, revealing its involvement in various aspects of the DNA damage response after exposure. <em>med17</em> mutant plants showed altered <em>HY5</em> expression, which encodes a transcription factor with a central role in photomorphogenesis. Our results demonstrate that <em>med17</em> mutants show altered photomorphogenic responses and also to darkness, when compared to WT plants, and these differences could be due to altered expression of genes encoding key regulators of light and darkness signaling pathways, such as <em>HY5</em>, <em>COP1</em> and <em>PIF3</em>. Moreover, <em>med17</em> mutants exhibit transcriptome changes similar to those previously reported in plants exposed to red and blue light, as well as those previously described for photoreceptor mutants. Interestingly, <em>med17</em> and <em>hy5</em> mutants show a similar set of differentially expressed genes compared to WT plants, which suggests that both proteins may participate in a common light and dark-induced signaling pathways. Together, our data provides evidence that MED17 is an important regulator of the light and darkness responses in Arabidopsis.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transition from vegetative growth to flowering is associated with suppression of the MUSA CENTRORADIALIS (MCN ) gene family in day neutral banana 香蕉从无性繁殖到开花的转变与日中性香蕉的 MUSA CENTRORADIALIS（MCN）基因家族的抑制有关。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-15 DOI: 10.1016/j.plantsci.2024.112289

Akhilesh K. Chaurasia , Hemant B. Patil , Bal Krishna , Vadakanthara R. Subramaniam , Prafullachandra V. Sane , Aniruddha P. Sane

{"title":"The transition from vegetative growth to flowering is associated with suppression of the MUSA CENTRORADIALIS (MCN ) gene family in day neutral banana","authors":"Akhilesh K. Chaurasia , Hemant B. Patil , Bal Krishna , Vadakanthara R. Subramaniam , Prafullachandra V. Sane , Aniruddha P. Sane","doi":"10.1016/j.plantsci.2024.112289","DOIUrl":"10.1016/j.plantsci.2024.112289","url":null,"abstract":"<div><div>Control over flowering time is essential for reproductive success and survival of plants. The <em>TERMINAL FLOWER1</em>/<em>CENTRORADIALIS/BROTHER OF FT AND TFL1</em> (<em>TFL1</em>/<em>CEN/BFT</em>) genes are key suppressor of flowering time that prevents premature conversion of the apical meristem into a floral meristem thereby allowing indeterminate vegetative growth. We have identified and characterized seven members of banana <em>TFL1/CEN/BFT</em> gene family (<em>MCN1–7</em>). All genes except <em>MCN6</em> show overlapping expression in the shoot apical meristem as well as leaves from the initial to mid-vegetative phases. Their expression is collectively reduced to their lowest just prior to flowering initiation at around 171 days, 226 days and 297 days, respectively, in three differently flowering varieties. Thereafter, there is steady increase in their transcript levels in the apical meristem as well as leaves that correlates with the development and growth of the inflorescence. The ability of three of the genes, <em>MCNs1–3</em>, to functionally complement the <em>tfl1–14</em> mutant of Arabidopsis provides additional evidence for structural and functional similarities of the <em>MCN</em> <!--> proteins to TFL1 even in a distantly related plant. Together, these results suggest that the MCN family in banana is associated with vegetative growth and suppression of flowering time initiation as well as indeterminate growth of inflorescence.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of genetic transformation system in Lilium pumilum and functional analysis of LpNAC6 on abiotic stress 百合遗传转化系统的建立及 LpNAC6 对非生物胁迫的功能分析

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-15 DOI: 10.1016/j.plantsci.2024.112292

Hongying Zhang , Hao Yan , Haitao Che , Kyongsok So , Longyi He , Yuxin Zhu , Bin Liu , Yanni Zhang

{"title":"Establishment of genetic transformation system in Lilium pumilum and functional analysis of LpNAC6 on abiotic stress","authors":"Hongying Zhang , Hao Yan , Haitao Che , Kyongsok So , Longyi He , Yuxin Zhu , Bin Liu , Yanni Zhang","doi":"10.1016/j.plantsci.2024.112292","DOIUrl":"10.1016/j.plantsci.2024.112292","url":null,"abstract":"<div><div><em>Lilium pumilum</em> is widely distributed in northeast Asia. It exhibits strong resistance and possesses high ornamental value. However, it currently lacks an efficient and stable transformation system. Therefore, we aimed to establish an effective genetic transformation system using the <em>Agrobacterium</em>-mediated method for <em>L. pumilum</em>, enabling gene transfer into the plant for gene function research and genetic engineering breeding. Our genetic transformation system achieved a transformation efficiency of 7.25 % under specific conditions: a kanamycin (Kana) concentration of 120 mg/L, 3 days of pre-cultivation, an <em>A. tumefaciens</em> concentration of 0.7 OD<sub>600</sub>, an acetosyringone (AS) concentration of 20 mg/L, and a 15-minute infection period. We investigated the function of the <em>LpNAC6</em> from <em>L. pumilum</em> by observing phenotypic and physiological changes under stresses induced by salt, alkali, and drought. Furthermore, overexpression of <em>LpNAC6</em> resulted in enhanced stress tolerance as evidenced by increased levels of SOD, POD, CAT enzymes, improved photosynthetic indices, and elevated chlorophyll contents; as well as reduced levels of MDA and reactive oxygen species (ROS). These findings demonstrate that we have successfully established a transgenic transformation method for <em>L. pumilum</em> while also providing essential information for cultivating stress-tolerant <em>Lilium</em> species and advancing our understanding of the functions of <em>LpNAC6</em> in plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prone to loss: Senescence-regulated protein degradation leads to lower protein extractability in aging tomato leaves 容易损失：衰老调节的蛋白质降解导致衰老番茄叶片中蛋白质提取率降低。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-15 DOI: 10.1016/j.plantsci.2024.112284

Marietheres Kleuter , Yafei Yu , Francesco Pancaldi , Atze Jan van der Goot , Luisa M. Trindade

{"title":"Prone to loss: Senescence-regulated protein degradation leads to lower protein extractability in aging tomato leaves","authors":"Marietheres Kleuter , Yafei Yu , Francesco Pancaldi , Atze Jan van der Goot , Luisa M. Trindade","doi":"10.1016/j.plantsci.2024.112284","DOIUrl":"10.1016/j.plantsci.2024.112284","url":null,"abstract":"<div><div>The utilization of proteins extracted from tomato (<em>Solanum lycopersicum</em>) leaves as cost-effective resources for human consumption or animal feed has gained interest. Thus, increasing protein extractability from tomato leaves became a new breeding target. However, the genetic factors influencing this trait remains poorly understood. In this study, we analyzed changes in leaf protein content, protein composition, and extraction yield across developmental stages, which are vegetative growth, flowering, fruit-forming, and mature fruit. Moreover, tomato gene expression across developmental stages was also studied, to identify genes underlying variability in leaf protein extraction. Protein extraction yield decreased from 0.51 g/g to 0.01 g/g leaf protein from the vegetative to mature stage. However, total protein content inferred with Dumas combustion analysis did not change over the developmental stages tested, while the protein-to-peptide ratio decreased significantly. To further analyze potential causes underlying the decline of protein-to-peptide ratio, the enzymatic activity of proteases – i.e. the enzymes responsible for protein degradation – and the expression of genes encoding these enzymes was studied along plant development. The overall specific activity of proteases did not change significantly throughout plant development. On the contrary, the gene expression of distinct members of the aspartic, cysteine, and subtilase protease families increased. Overall, our findings suggest that extraplastidic protein degradation likely underlies the protein degradation observed during senescence. In the future, the reduction of the activity of extraplastidic proteases through biotechnology could represent an effective strategy to develop tomato varieties with improved protein extraction yields.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of OsGASR1 promotes Al tolerance in rice OsGASR1 的过表达可促进水稻对 Al 的耐受性。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-15 DOI: 10.1016/j.plantsci.2024.112294

Shuling Cao , Liyun Peng , Jinyu Yu , Ziheng Li , Zhigang Wang , Dan Ma , Xiaoqian Sun , Huawei Zheng , Baolei Zhang , Xingxiang Chen , Zhufeng Chen , Jixing Xia

{"title":"Overexpression of OsGASR1 promotes Al tolerance in rice","authors":"Shuling Cao , Liyun Peng , Jinyu Yu , Ziheng Li , Zhigang Wang , Dan Ma , Xiaoqian Sun , Huawei Zheng , Baolei Zhang , Xingxiang Chen , Zhufeng Chen , Jixing Xia","doi":"10.1016/j.plantsci.2024.112294","DOIUrl":"10.1016/j.plantsci.2024.112294","url":null,"abstract":"<div><div>Aluminum (Al) toxicity in acid soils poses a significant threat to rice, which exhibits highly complex genetic mechanisms for both external detoxification and internal tolerance among cereal crops. Although several genes involved Al tolerance have been identified, the molecular mechanisms underlying Al tolerance in rice remain to be fully explored. Here, we functionally characterized the gibberellin-stimulated transcription gene <em>OsGASR1</em>, which encodes a small cysteine-rich peptide localized to the nucleus and cytoplasm and plays a significant role in Al tolerance in rice. The expression of <em>OsGASR1</em> is rapidly up-regulated by Al in rice root tips but not in the shoots. Its expression is not regulated by the central regulator Aluminum Resistance Transcription Factor 1 (ART1), indicating that <em>OsGASR1</em> functions as a novel gene in rice Al resistance independent of ART1. Knockout of <em>OsGASR1</em> reduced root length but did not affect Al tolerance in rice, whereas overexpression of <em>OsGASR1</em> enhanced Al tolerance without affecting Al distribution and accumulation and promoted the accumulation of reactive oxygen species (ROS) in the root tips. RNA-seq analysis revealed that overexpression of <em>OsGASR1</em> upregulated the expression of genes associated with cell wall modification, oxidative stress, and Al tolerance. Collectively, these findings suggest that OsGASR1 is involved in Al tolerance in rice independently of ART1, and the up-regulation of this gene is necessary for rice Al tolerance.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methyltransferase 1 (OsMTS1) interacts with hydroxycinnamoyltransferase 1 (OsHCT1) and promotes heading by upregulating heading date 1 (Hd1) 甲基转移酶 1（OsMTS1）与羟基肉桂酰基转移酶 1（OsHCT1）相互作用，通过上调标题日期 1（Hd1）促进标题。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-15 DOI: 10.1016/j.plantsci.2024.112291

Amir Sohail

{"title":"Methyltransferase 1 (OsMTS1) interacts with hydroxycinnamoyltransferase 1 (OsHCT1) and promotes heading by upregulating heading date 1 (Hd1)","authors":"Amir Sohail","doi":"10.1016/j.plantsci.2024.112291","DOIUrl":"10.1016/j.plantsci.2024.112291","url":null,"abstract":"<div><div>Heading date determines the distribution and yield potentials of rice, and is an ideal target for crop improvement using CRISPR/Cas9 genome editing system. In this study, we reported the loss-of-function of <em>Methyltransferase 1</em> (<em>MTS1</em>), which promotes heading in rice. Here, we constructed knockouts and overexpression transgenic plants of <em>OsMTS1</em> in ZH8015 and Nipponbare (NIP) for the first time to validate its heading date function in rice subspecies <em>Oryza sativa ssp. Indica</em> and <em>O. Sativa ssp. Japonica</em>, respectively. The <em>OsMTS1</em> knockouts in ZH8015 and NIP rice significantly promoted heading date under both natural short days (NSD) and natural long days (NLD) conditions, while the overexpression of <em>OsMTS1</em> significantly delayed heading date in ZH8015 and NIP rice under both NSD and NLD conditions. Likewise, the complementation transgenic plants displayed late heading date phenotype. <em>OsMTS1</em> repressed heading through up-regulating <em>Heading date 1</em> (<em>Hd1</em>) and down-regulating <em>Early heading date 1</em> (<em>Ehd1</em>) and <em>Heading date 3a</em> (<em>Hd3a</em>). The OsMTS1 protein interacted with OsHCT1 proteins using a yeast two-hybrid (Y2H) assay. The Y2H and overexpression confirmed that OsMTS1 interacted with <em>OsHCT1</em>, which delayed heading by 4.7 days under NLD. Taken together, CRISPR/Cas9, genetic complementation, and overexpression results validated that <em>OsMTS1</em> represses heading in <em>Indica</em> and <em>Japonica</em> rice under both NLD and NSD conditions. These results demonstrated that <em>OsMTS1</em> is a useful target for breeding early maturing rice varieties by CRISPR/Cas9 gene editing of the functional allele.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VvbZIP22 regulates quercetin synthesis to enhances cold resistance in grape VvbZIP22 可调节槲皮素的合成，从而增强葡萄的抗寒性。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-10-15 DOI: 10.1016/j.plantsci.2024.112293

Guangchao Liu , Zhe Zhang , Ye Tian , Jie Yang , Xingfeng Xu , Xin Liu

{"title":"VvbZIP22 regulates quercetin synthesis to enhances cold resistance in grape","authors":"Guangchao Liu , Zhe Zhang , Ye Tian , Jie Yang , Xingfeng Xu , Xin Liu","doi":"10.1016/j.plantsci.2024.112293","DOIUrl":"10.1016/j.plantsci.2024.112293","url":null,"abstract":"<div><div>Grapes are one of the important fruit crops widely cultivated in the world, with high nutritional and economic value. However, with the intensification of global warming, extreme low temperature has seriously affected the development of the grape industry. Quercetin is a highly antioxidant active substance that can enhance the tolerance of plants to external environmental stress, but its function and mechanism in response to low-temperature stress in grapes are still unclear. Here, we found that grapes accumulate more quercetin under low-temperature stress, and exogenous quercetin can significantly improve the cold resistance of grapes. The key quercetin synthesis gene <em>VvFLS1</em> (<em>flavanol synthase 1</em>) is up-regulated after low-temperature treatment, and overexpression of <em>VvFLS1</em> increases quercetin content and enhances the cold resistance of grape. Yeast one-hybrid and dual luciferase reporter systems demonstrate that VvbZIP22 (<em>basic-leucine zipper 22</em>) directly binds to the <em>VvFLS1</em> promoter, and VvbZIP22 has cold-induced expression characteristics. Overexpression of VvbZIP22 significantly improves the cold resistance of grape. The above results indicate that quercetin plays an important role in the response of grapes to low-temperature stress. Under low temperature, VvbZIP22 can mediate quercetin synthesis through regulating <em>VvFLS1</em>, alleviate oxidative damage, and improve the cold resistance of grapes.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0