SlMADS50是一种I型MADS-box转录因子，通过激素途径调控番茄植株结构

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-10-10 DOI:10.1016/j.plantsci.2025.112811

Xuhu Guo , Lingzhi Li , Zhiping Han , Jun Zhang , Jingjing Song , Shitong Zhou

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引用次数: 0

摘要

MADS-box基因调控植物的生长发育。我们克隆了1型MADS-box基因SlMADS50，该基因在番茄根、叶和侧芽中表达量较高，可能参与番茄营养器官的生长调控。亚细胞定位结果表明，SlMADS50蛋白位于细胞核内。本研究以经典番茄品种Ailsa Craig （ac++）为背景材料，通过RNA干扰对SlMADS50基因进行了沉默。与野生型相比，slmads50沉默系表现出较低的顶端优势和较低的株高。叶片的长度、宽度、周长和面积均小于野生型植物。根的总长度、总表面积、总投影面积、体积、分叉和根尖均显著减小。解剖研究表明，slmads50沉默的茎纵切面细胞较小，但平均细胞数明显增加。在激素水平下，slmads50沉默系的IAA（吲哚-3-乙酸）、GA3（赤霉素A3）、转玉米糖苷（TZR）和CS （castasterone）含量降低。在分子水平上，生长素反应基因SlIAA3和赤霉素合成基因SlGA3ox1在slmads50沉默系中显著下调，而GA信号负调控基因SlDELLA在沉默系中显著上调。基于酵母双杂交实验显示SlMADS50与RBCS3和PUB22-like单独相互作用，我们进一步推测SlMADS50可能通过光合作用和激素途径调节番茄植株结构。本研究阐明了SlMADS50基因在番茄植株结构调控中的生物学功能及其调控网络。这一发现不仅促进了对番茄I型MADS-box基因的认识，也为进一步研究番茄植株结构调控提供了可靠的理论基础。

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SlMADS50, A Type I MADS-box transcription factor, regulates tomato plant architecture via hormonal pathways

The MADS-box gene regulates plant growth and development. We cloned the type I MADS-box gene SlMADS50, which is relatively highly expressed in tomato roots, leaves and lateral buds, suggesting that it may be involved in the growth regulation of tomato vegetative organs. Subcellular localization results showed that the SlMADS50 protein was located in the nucleus. In this study, the classical tomato cultivar Ailsa Craig (AC⁺⁺) was used as background material to silence SlMADS50 gene by RNA interference. Compared with the wild type, SlMADS50-silenced lines exhibited lower apical dominance and reduced plant height. The length, width, perimeter and area of leaves were smaller than wild type plants. The total length, total surface area, total projected area, volume, forks and tips of roots were significantly reduced. Anatomical studies showed that the cells in the longitudinal section of the SlMADS50-silenced stem were smaller, but their average number was significantly increased. At the hormonal levels, the contents of IAA (indole-3-acetic acid), GA3(gibberellin A3), TZR (transzein riboside) and CS (castasterone) in SlMADS50-silenced lines were decreased. At the molecular level, auxin response gene SlIAA3 and gibberellin synthesis genes SlGA3ox1 were significantly down-regulated in SlMADS50-silenced lines, while the negative regulator of GA signaling, SlDELLA, were significantly up-regulated in the silenced lines. Based on the yeast two-hybrid assay showing that SlMADS50 interacts individually with RBCS3 and PUB22-like, we further speculate that SlMADS50 may regulate tomato plant architecture through photosynthetic and hormonal pathways. This study elucidated the biological function of SlMADS50 and its regulatory network in controlling tomato plant architecture. The findings not only advanced the understanding of type I MADS-box genes in tomato but also provide a reliable theoretical foundation for further research on plant architecture regulation in tomato.

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.