Plant Science最新文献

{"title":"Carotenoid biosynthesis profiling unveils the variance of flower coloration in Tagetes erecta and enhances fruit pigmentation in tomato","authors":"Yaqiong Qiu ,&nbsp;Ruipeng Wang ,&nbsp;Enqi Zhang ,&nbsp;Yafang Shang ,&nbsp;Guodong Feng ,&nbsp;Wenjing Wang ,&nbsp;Yilong Ma ,&nbsp;Wenbo Bai ,&nbsp;Wan Zhang ,&nbsp;Zhiqiang Xu ,&nbsp;Wei Shi ,&nbsp;Xiangli Niu","doi":"10.1016/j.plantsci.2024.112207","DOIUrl":"10.1016/j.plantsci.2024.112207","url":null,"abstract":"<div><p>Carotenoids play a pivotal role in plant. <em>Tagetes erecta</em>, commonly called marigold, has increasing nutritional and economic value due to its high level of carotenoids in flower. However, the functional genes in the carotenoid biosynthesis of <em>T. erecta</em> have not been studied. In this work, three <em>T. erecta</em> varieties with flowers of yellow, yellow-orange and orange color, respectively, were examined for carotenoids composition and corresponding expression profiling of biosynthetic genes at four developmental stages. The results indicated that the varieties with higher lutein content, orange-flower ‘Juwang’ and yellow-orange ‘Taishan’, exhibited significant upregulation of genes in the upstream biosynthesis pathway, especially <em>PDS</em> (<em>phytoene desaturase</em>), <em>PSY</em> (<em>phytoene synthase</em>) and <em>ZDS</em> (<em>zeta-carotene desaturase</em>), whereas downstream carotenoid cleavage genes <em>CCD</em> (<em>carotenoid cleavage dioxygenase</em>) were markedly downregulated throughout flower development in the highest lutein containing variety ‘Juwang’. Furthermore, marigold <em>TePDS</em>, <em>TePSYS3</em> and <em>TeZDS</em> were isolated and transformed into tomato. Overexpression of <em>TePDS</em> or <em>TeZDS</em> resulted in the promotion of fruit ripening and accumulation of carotenoids in the transgenic lines. On the other hand, marigold <em>TePSYS3</em> showed multiple effects, not only on fruit carotenogenesis but also on pigmentation patterns in vegetative tissues and plant growth. Taken together, the variations in expression profiles of the biosynthetic genes contribute to dynamic change in carotenoid levels and diversity of flower coloration in <em>T. erecta.</em> These functional genes of <em>T. erecta</em> were verified in tomato and provide targets for genetic improvement of fruit carotenoids accumulation.</p></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"347 ","pages":"Article 112207"},"PeriodicalIF":4.2,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168945224002346/pdfft?md5=9696138c9879e799f104c358c2c7a02c&pid=1-s2.0-S0168945224002346-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Family ties: Root-root communication within Solanaceae","authors":"Milena Maria Tomaz de Oliveira ,&nbsp;Aye Nyein Ko ,&nbsp;Sophie Obersteiner ,&nbsp;Omer Falik ,&nbsp;Shimon Rachmilevitch","doi":"10.1016/j.plantsci.2024.112203","DOIUrl":"10.1016/j.plantsci.2024.112203","url":null,"abstract":"<div><p>Root–root communication effects on several physiological and metabolic aspects among Solanaceae relatives were studied. We examined cherry (C) and field (F) tomato (<em>Solanum lycopersicum</em>) and bell pepper (B) (<em>Capsicum annuum</em>), comprising three degrees of relatedness (DOR): high (H-DOR; CC, FF and BB), medium (M-DOR; CF) and low (L-DOR; CB and FB). Plants were grown in pairs of similar or different plants on a paper-based and non-destructive root growth system, namely, rhizoslides. Root growth, including the proliferation of fine roots, and respiration increased as the DOR decreased and were highest in paired L-DOR plants, as was shown for root respiration that increased by 63, 110 and 88 % for C, F, and B when grown with B, B and F, respectively. On the other hand, root exudates of L-DOR plants had significantly lower levels of total organic carbon and protein than those of H-DOR plants, indicating different root–root communication between individuals with different DOR. Our findings indicate, for the first time, that carbon allocation to root growth, exudation and respiration depends on the degree of genetic relatedness, and that the degree of relatedness between individual plants plays a key role in the root-root communication within Solanaceae.</p></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"347 ","pages":"Article 112203"},"PeriodicalIF":4.2,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amino acid permease OsAAP12 negatively regulates rice tillers and grain yield by transporting specific amino acids to affect nitrogen and cytokinin pathways","authors":"Feng Jin ,&nbsp;Weiting Huang ,&nbsp;Pengfei Xie ,&nbsp;Bowen Wu,&nbsp;Quanzhi Zhao,&nbsp;Zhongming Fang","doi":"10.1016/j.plantsci.2024.112202","DOIUrl":"10.1016/j.plantsci.2024.112202","url":null,"abstract":"<div><p>Amino acids are necessary nutrients for the growth of <em>Oryza sativa</em> (rice), which can be mediated by amino acid transporter; however, our understanding of these transporters is still limited. This study found that the expression levels of amino acid permease gene <em>OsAAP12</em> differed between <em>indica</em> and <em>japonica</em> rice. Altered expression of <em>OsAAP12</em> negatively regulated tillering and yield in transgenic rice lines. Subcellular localization revealed that OsAAP12 was primarily localized to the plasma membrane. Moreover, it was indicated that OsAAP12 transported polar neutral amino acids asparagine (Asn), threonine (Thr), and serine (Ser) through experiments involving yeast heterologous complementation, fluorescence amino acid uptake, and amino acid content determination. Additionally, exogenous application of amino acids Asn, Thr, and Ser suppressed axillary buds outgrowth in <em>OsAAP12</em> overexpression lines compared with wild-type ZH11. Conversely, the opposite trend was observed in CRISPR mutant lines. RNA-seq analysis showed that the expression patterns of genes involved in the nitrogen and cytokinin pathways were generally altered in <em>OsAAP12</em> modified lines. Hormone assays indicated that <em>OsAAP12</em> mutant lines accumulated cytokinins in the basal part of rice, whereas overexpression lines had the opposite effect. In summary, CRISPR mutant of <em>OsAAP12</em> boosted rice tillering and grain yield by coordinating the content of amino acids and cytokinins, which has potential application value in high-yield rice breeding.</p></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"347 ","pages":"Article 112202"},"PeriodicalIF":4.2,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of glucosinolates and phenolics in the promotion of broccoli seedling growth through the modulation of primary and secondary metabolism","authors":"Lorena Albaladejo-Marico,&nbsp;Micaela Carvajal,&nbsp;Lucia Yepes-Molina","doi":"10.1016/j.plantsci.2024.112205","DOIUrl":"10.1016/j.plantsci.2024.112205","url":null,"abstract":"<div><p>Secondary metabolites play an essential role in plant defense. However, the role of glucosinolates and phenols in brassica crop yield in the context of environmentally friendly agricultural practices has not been established. Our study investigated the effects of a Brassica extract, rich in these metabolites, on the physiology and metabolism of broccoli (<em>Brassica oleracea</em> L. <em>var. italica</em>) seedlings and the subsequent development of the plants in adult stages. The results showed an increase in growth in the extract-treated seedlings, which was associated with an alteration of primary and secondary metabolism. In particular, there was an increase in the levels of amino acids, phenolic compounds and hormones, while the levels of glucosinolates decreased. Lipid peroxidation diminished in treated plants, indicating improved membrane integrity. Treated plants subsequently grown in hydroponically showed increased water use efficiency, transpiration, and internal carbon, which contributed to the improved growth of these plants. Overall, our findings underscore the potential of the glucosinolates and phenols ratio as essential to improve crop growth and stress tolerance, as well as revealed the interest of studying the mechanisms involved in the possible uptake and integration of GSLs by broccoli seedlings after external application.</p></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"347 ","pages":"Article 112205"},"PeriodicalIF":4.2,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168945224002322/pdfft?md5=7f3e7e00748806636b553ac65d19144c&pid=1-s2.0-S0168945224002322-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PoARRO-1 regulates adventitious rooting through interaction with PoIAA27b in Paeonia ostii","authors":"Jiange Wang ,&nbsp;Yinglong Song ,&nbsp;Guiqing Wang ,&nbsp;Liyun Shi ,&nbsp;Yuxiao Shen ,&nbsp;Weichao Liu ,&nbsp;Yufeng Xu ,&nbsp;Xueyuan Lou ,&nbsp;Wenqing Jia ,&nbsp;Minhuan Zhang ,&nbsp;Wenqian Shang ,&nbsp;Songlin He ,&nbsp;Zheng Wang","doi":"10.1016/j.plantsci.2024.112204","DOIUrl":"10.1016/j.plantsci.2024.112204","url":null,"abstract":"<div><p>Adventitious root (AR) formation is a limiting factor in the vegetative propagation of tree peony (<em>Paeonia suffruticosa</em> Andr.). <em>PoARRO-1</em>, which encodes an auxin oxidase involved in AR formation, plays a role in the root development of <em>P. ostii</em>, but its associated molecular regulatory mechanisms are not yet understood. In this study, we examined the role of <em>PoARRO-1</em> in AR formation in <em>P. ostii</em>. The overexpression of <em>PoARRO-1</em> in <em>P. ostii</em> test-tube plantlets led to a notable enhancement in both the rooting rate and the average number of ARs <em>in vitro,</em> as well as increased activities of peroxidase (POD), superoxide dismutase (SOD), and indoleacetic acid oxidase (IAAO). <em>PoARRO-1</em> was involved in the conversion of IAA-Asp and IAA-Glu to OxIAA and promoted IAA oxidation. RNA sequencing analysis revealed that <em>PoARRO-1</em> overexpression led to upregulation of enzyme activity, auxin metabolism related genes. Further analyses showed that PoARRO-1 interacted with the 1–175 aa position of PoIAA27b to regulate the formation of ARs. We therefore propose that PoARRO-1 interacts with PoIAA27b to promote AR formation, and it may be useful targets for enhancing the <em>in vitro</em> propagation of <em>P. ostii</em>.</p></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"347 ","pages":"Article 112204"},"PeriodicalIF":4.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141767175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surviving the desert's grasp: Decipherment phreatophyte Tamarix aphylla (L.) Karst. Adaptive strategies for arid resilience","authors":"Ummar Iqbal,&nbsp;Ali Daad,&nbsp;Ahmad Ali,&nbsp;Muhammad Faisal Gul,&nbsp;Muhammad Usama Aslam,&nbsp;Fahad Ur Rehman,&nbsp;Umar Farooq","doi":"10.1016/j.plantsci.2024.112201","DOIUrl":"10.1016/j.plantsci.2024.112201","url":null,"abstract":"<div><p>Phreatophytes play an important role in maintaining the ecological services in arid and semi-arid areas. Characterizing the interaction between groundwater and phreatophytes is critical for the land and water management in such areas. Therefore, the identification of key traits related to mitigating desertification in differently adapted <em>T. aphylla</em> populations was the focus. Fifteen naturally adapted populations of the prominent phreatophyte <em>T. aphylla</em> from diverse ecological regions of Punjab, Pakistan were selected. Key structural and functional modifications involved in ecological success and adaptations against heterogeneous environments for water conservation include widened metaxylem vessels in roots, enlarged brachy sclereids in stems/leaves, tissues succulence, and elevated organic osmolytes and antioxidants activity for osmoregulation and defense mechanism. Populations from hot and dry deserts (D_{<em>ratio</em>}: 43.17−34.88) exhibited longer roots and fine-scaled leaves, along with enlarged vascular bundles and parenchyma cells in stems. Populations inhabiting saline deserts (D_{<em>ratio</em>}: 38.59−33.29) displayed enhanced belowground biomass production, larger root cellular area, broadest phloem region in stems, and numerous large stomata in leaves. Hyper-arid populations (D_{<em>ratio</em>}: 33.54−23.07) excelled in shoot biomass production, stem cellular area, epidermal thickness, pith region in stems, and lamina thickness in leaves. In conclusion, this research highlights <em>T. aphylla</em> as a vital model for comprehending plant resilience to environmental stresses, with implications for carbon sequestration and ecosystem restoration.</p></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"347 ","pages":"Article 112201"},"PeriodicalIF":4.2,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ILP1 and NTR1 affect the stability of U6 snRNA during spliceosome complex disassembly in Arabidopsis","authors":"Jiaming Wu ,&nbsp;Wei Chen ,&nbsp;Shengchao Ge ,&nbsp;Xueliang Liu ,&nbsp;Junling Shan ,&nbsp;Meishan Zhang ,&nbsp;Yuan Su ,&nbsp;Yunfeng Liu","doi":"10.1016/j.plantsci.2024.112199","DOIUrl":"10.1016/j.plantsci.2024.112199","url":null,"abstract":"<div><p>U6 snRNA is one of the uridine-rich non-coding RNAs, abundant and stable in various cells, function as core particles in the intron-lariat spliceosome (ILS) complex. The Increased Level of Polyploidy1–1D (ILP1) and NTC-related protein 1 (NTR1), two conserved disassembly factors of the ILS complex, facilitates the disintegration of the ILS complex after completing intron splicing. The functional impairment of ILP1 and NTR1 lead to increased U6 levels, while other snRNAs comprising the ILS complex remained unaffected. We revealed that ILP1 and NTR1 had no impact on the transcription, 3’ end phosphate structure or oligo(U) tail of U6 snRNA. Moreover, we uncovered that the mutation of ILP1 and NTR1 resulted in the accumulation of ILS complexes, impeding the dissociation of U6 from splicing factors, leading to an extended half-life of U6 and ultimately causing an elevation in U6 snRNA levels. Our findings broaden the understanding of the functions of ILS disassembly factors ILP1 and NTR1, and providing insights into the dynamic disassembly between U6 and ILS.</p></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"347 ","pages":"Article 112199"},"PeriodicalIF":4.2,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress induced factor 2 is a dual regulator for defense and seed germination in Arabidopsis","authors":"Ching Chan ,&nbsp;Yi-Jun Liao,&nbsp;Shian-Peng Chiou","doi":"10.1016/j.plantsci.2024.112200","DOIUrl":"10.1016/j.plantsci.2024.112200","url":null,"abstract":"<div><p>Receptor-like kinases (RLKs) constitute a diverse superfamily of proteins pivotal for various plant physiological processes, including responses to pathogens, hormone perception, growth, and development. Their ability to recognize conserved epitopes for general elicitors and specific pathogens marked significant advancements in plant pathology research. Emerging evidence suggests that RLKs and associated components also act as modulators in hormone signaling and cellular trafficking, showcasing their multifunctional roles in growth and development. Notably, STRESS INDUCED FACTOR 2 (SIF2) stands out as a representative with distinct expression patterns in different Arabidopsis organs. Our prior work highlighted the specific induction of <em>SIF2</em> expression in guard cells, emphasizing its positive contribution to stomatal immunity. Expanding on these findings, our present study delves into the diverse functions of <em>SIF2</em> expression in root tissues. Utilizing comprehensive physiology, molecular biology, protein biochemistry, and genetic analyses, we reveal that SIF2 modulates abscisic acid (ABA) signaling in Arabidopsis roots. SIF2 is epistatic with key regulators in the ABA signaling pathway, thereby governing the expression of genes crucial for dormancy release and, consequently, Arabidopsis seed germination. This study sheds light on the intricate roles of SIF2 as a multi-functional RLK, underscoring its organ-specific contributions to plant immunity, hormonal regulation, and seed germination.</p></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"347 ","pages":"Article 112200"},"PeriodicalIF":4.2,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AtDREB2G is involved in the regulation of riboflavin biosynthesis in response to low-temperature stress and abscisic acid treatment in Arabidopsis thaliana","authors":"Junya Namba ,&nbsp;Miho Harada ,&nbsp;Rui Shibata ,&nbsp;Yuina Toda ,&nbsp;Takanori Maruta ,&nbsp;Takahiro Ishikawa ,&nbsp;Shigeru Shigeoka ,&nbsp;Kazuya Yoshimura ,&nbsp;Takahisa Ogawa","doi":"10.1016/j.plantsci.2024.112196","DOIUrl":"10.1016/j.plantsci.2024.112196","url":null,"abstract":"<div><p>Riboflavin (RF) serves as a precursor to flavin mononucleotide and flavin adenine dinucleotide, which are crucial cofactors in various metabolic processes. Strict regulation of cellular flavin homeostasis is imperative, yet information regarding the factors governing this regulation remains largely elusive. In this study, we first examined the impact of external flavin treatment on the <em>Arabidopsis</em> transcriptome to identify novel regulators of cellular flavin levels. Our analysis revealed alterations in the expression of 49 putative transcription factors. Subsequent reverse genetic screening highlighted a member of the dehydration-responsive element binding (DREB) family, AtDREB2G, as a potential regulator of cellular flavin levels. Knockout mutants of <em>AtDREB2G</em> (<em>dreb2g</em>) exhibited reduced flavin levels and decreased expression of RF biosynthetic genes compared to wild-type plants. Conversely, conditional overexpression of <em>AtDREB2G</em> led to an increase in the expression of RF biosynthetic genes and elevated flavin levels. In wild-type plants, exposure to low temperatures and abscisic acid treatment stimulated enhanced flavin levels and upregulated the expression of RF biosynthetic genes, concomitant with the induction of <em>AtDREB2G</em>. Notably, these responses were significantly attenuated in <em>dreb2g</em> mutants. Our findings establish AtDREB2G is involved in the positive regulation of flavin biosynthesis in <em>Arabidopsis</em>, particularly under conditions of low temperature and abscisic acid treatment.</p></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"347 ","pages":"Article 112196"},"PeriodicalIF":4.2,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141637595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of R2R3-MYB family in Eriobotrya japonica and functional analysis of EjMYB5 involved in proanthocyanidin biosynthesis","authors":"Yin Zhang ,&nbsp;Luyan Hu ,&nbsp;Shuming Wang ,&nbsp;Xiuhong Gou ,&nbsp;Qigao Guo ,&nbsp;Guolu Liang","doi":"10.1016/j.plantsci.2024.112198","DOIUrl":"10.1016/j.plantsci.2024.112198","url":null,"abstract":"<div><p>Loquat (<em>Eriobotrya japonica</em> Lindl.) is a popular fruit and medicinal plant. Proanthocyanidins (PAs), as one of the main types of flavonoids, are the key components of loquat fruit quality and medicinal properties. However, the identification of transcription factors (TFs) involved in PA accumulation in loquat remains limited. R2R3-MYB TFs play key regulatory role in PA accumulation in plants. In this study, 190 R2R3-MYB TFs were identified in loquat genome. Combined with transcriptome data, R2R3-MYB TF EjMYB5 involved in PA accumulation in loquat was isolated. EjMYB5 was transcriptional activator localized to nucleus. Expression of <em>EjMYB5</em> was closely related to PA accumulation in loquat fruits. Heterogenous overexpression of <em>EjMYB5</em> in tomato (<em>Solanum lycopersicum</em>) inhibited anthocyanin accumulation and promoted PA accumulation. Additionally, transient overexpression of <em>EjMYB5</em> in tobacco (<em>Nicotiana benthamiana</em>) leaves promoted PA accumulation by upregulating flavonoid biosynthesis genes (<em>NtDFR</em>, <em>NtANS</em>, and <em>NtLAR</em>). Transcriptome analysis of <em>EjMYB5</em>-overexpressing tomato fruits suggested that EjMYB5 was involved in several biological pathways, including lipid metabolism, MAPK signaling, phenylpropanoid biosynthesis, and flavonoid biosynthesis. Collectively, our findings provided basic data for further analysis the function of R2R3-MYB TFs in loquat, and revealed that EjMYB5 functioned as PA accumulation in loquat.</p></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"347 ","pages":"Article 112198"},"PeriodicalIF":4.2,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141727637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}