Plant Science最新文献

{"title":"The role of multiple C2 domain and transmembrane region proteins in mediating tomato development","authors":"Siyi Li ,&nbsp;Ribin Ling ,&nbsp;Xuexia Wu ,&nbsp;Lu Liu ,&nbsp;Hui Zhang ,&nbsp;Lijie Xuan","doi":"10.1016/j.plantsci.2025.112511","DOIUrl":"10.1016/j.plantsci.2025.112511","url":null,"abstract":"<div><div>Multiple C2 domain and transmembrane region proteins (MCTPs) are an evolutionarily conserved family involved in protein trafficking and signal transduction. Although several investigations have demonstrated that MCTPs play crucial roles in plant growth and development, their specific biological functions within tomatoes (<em>Solanum lycopersicum</em> L.) remain predominantly mysterious. In this study, we identify and characterize 14 <em>SlMCTP</em> genes derived from tomatoes. Chromosome mapping, gene structure, phylogenetic connections, and subcellular localization are presented herein. Meanwhile, the varied expression patterns of <em>SlMCTPs</em> within different tissues and under diverse hormonal and NaCl treatment conditions are revealed. Moreover, we find that <em>SlMCTP10</em>, <em>SlMCTP11</em>, and <em>SlMCTP12</em>, which belong to the same clade, display high expression levels at the main stem apex, suggesting their potential functions in shoot development. Furthermore, we knock out the <em>SlMCTP10</em> gene in tomato using CRISPR-Cas9. The <em>Slmctp10</em> seedlings exhibit defects in shoot meristem development, manifested by abnormal cotyledons and shorter internodes. Together, our findings offer fundamental insights into the SlMCTP family and uncover the role of SlMCTP proteins in regulating shoot meristem development in tomato plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112511"},"PeriodicalIF":4.2,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional analysis of BAG gene family contributing to verticillium wilt resistance in upland cotton","authors":"Zhijuan Hu ,&nbsp;Jingjie Yuan ,&nbsp;Run Zou ,&nbsp;Yilan Wang ,&nbsp;Xuan Peng ,&nbsp;Xingyong Yang ,&nbsp;Chengjian Xie","doi":"10.1016/j.plantsci.2025.112501","DOIUrl":"10.1016/j.plantsci.2025.112501","url":null,"abstract":"<div><div>Cotton fiber is a primary textile material and a significant economic resource globally. <em>Verticillium dahliae</em>, a destructive soil-borne fungal pathogen, severely impacts cotton yields. The Bcl-2–associated athanogene (BAG) protein family, functioning as molecular chaperone co-chaperones, plays a crucial role in plant stress responses. In this study, 24, 12, and 11 <em>BAG</em> genes were identified in upland cotton (<em>Gossypium hirsutum</em>), Asiatic cotton (<em>G. arboreum</em>), and Levant cotton (<em>G. raimondii</em>), respectively. The <em>BAG</em> gene family demonstrates relative conservation throughout cotton evolution. Conserved domain analysis revealed that BAG proteins from these species universally contain the conserved BAG domain, with some members also possessing the UBL domain and CaM-binding motifs. Virus-induced gene silencing (VIGS) was utilized to investigate gene function in upland cotton. Compared to the negative control, following <em>V. dahliae</em> infection, the silencing of GhBAG7.1 and GhBAG6.2 makes the plants more susceptible to infection, showing symptoms earlier. Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) analysis indicated that <em>V. dahliae</em> infection upregulated the expression of <em>GhBAG7.1, GhBAG6.2</em>, and <em>GhBAG4.1</em> in upland cotton, while <em>GhBAG4.4</em> expression was downregulated. Furthermore, following the silencing of the <em>GhBAG6.2</em> gene, <em>V. dahliae</em> infection led to an initial upregulation of disease resistance-related genes (<em>ERF1</em>, <em>PR5</em>, <em>PDF1.2</em>, <em>NPR1</em>, <em>PR1</em>, <em>OPR3</em>), which was followed by a subsequent decrease in their expression. Transcriptomic analysis revealed a transient upregulation of defense-related pathways, including phenylpropanoid biosynthesis, MAPK signaling pathway, and plant-pathogen interactions, at 48- and 96-hours post-inoculation with <em>V. dahliae</em>. The findings provide a foundation for future research on stress-tolerant genes in cotton and offer new genetic resources for breeding disease-resistant cotton varieties.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112501"},"PeriodicalIF":4.2,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive metabolomics and transcriptomics analysis reveal the regulatory mechanism of StHY5 on anthocyanin accumulation in potato tubers","authors":"Huiling Zhang ,&nbsp;Jingjing Liu ,&nbsp;Yanan Zhao ,&nbsp;Enshuang Wang ,&nbsp;Jianhua Zhou ,&nbsp;Kaixin Chang ,&nbsp;Xijuan Zhao ,&nbsp;Botao Song","doi":"10.1016/j.plantsci.2025.112500","DOIUrl":"10.1016/j.plantsci.2025.112500","url":null,"abstract":"<div><div>Light is a key factor for inducing anthocyanin biosynthesis; however, its regulatory mode in potato anthocyanin biosynthesis remains unclear. Previous research identified a specific genotype that causes the tuber skin to gradually turn purple when exposed to light of different wavelengths. In the present study, we conducted metabolome and transcriptome analyses on tuber samples during anthocyanin accumulation. The metabolome data showed that the contents of naringenin chalcone, naringenin, dihydrokaempferol, and cyanidin gradually increased during anthocyanin accumulation. The transcriptome data showed that the expression levels of most structural genes increased gradually during anthocyanin accumulation, especially the <em>StF3’H</em> gene that promotes cyanidin formation. Moreover, the photo-responsive transcription factor <em>StHY5</em> was specifically expressed at high levels before anthocyanin accumulation, occurring 2 h after light induction. Establishment of transgenic lines demonstrated that StHY5 overexpression could promote the accumulation of anthocyanin in potato tubers, along with a parallel increase in the transcription levels of <em>StAN2</em>, <em>StMYBA1</em>, <em>StCHI</em>, <em>StF3H</em>, <em>StF3’H</em>, and <em>StDFR</em>. Electrophoretic mobility shift and dual luciferase assays showed that StHY5 can enhance the promoter activity of the MYB transcription factors <em>StAN2</em> and <em>StMYBA1</em> as well as the structural genes <em>StCHI</em> and <em>StF3H</em> through binding to the G-box motif. StAN2 activated the expression of <em>StF3’H</em> (a newly identified purple gene locus in potato) and <em>StDFR</em> by binding to the MYB-binding site in the promoters, thereby promoting anthocyanin biosynthesis. This study provides a theoretical basis for revealing the molecular mechanism of light-regulated anthocyanin biosynthesis in potatoes.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112500"},"PeriodicalIF":4.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of maize transcription factor ZmNF-YC14 positively regulates drought and salt stress responses in Arabidopsis thaliana","authors":"Yimeng Wang ,&nbsp;Peng Jiao ,&nbsp;Chunlai Wang ,&nbsp;Chenyang Wu ,&nbsp;Xiaotong Wei ,&nbsp;Siyan Liu ,&nbsp;Yiyong Ma ,&nbsp;Shuyan Guan","doi":"10.1016/j.plantsci.2025.112502","DOIUrl":"10.1016/j.plantsci.2025.112502","url":null,"abstract":"<div><div>Maize (<em>Zea mays</em> L.) is a food crop with the largest planted area globally and one of the highest total yields worldwide. However, in recent years, deteriorating climate, increasing scarcity of freshwater resources, and rising land salinity have caused drought and salinity stress to be the two major factors that restrict crop growth, development, and yield, significantly affecting crop production and ecological sustainability. Nuclear factor Ys (NF-Ys) are an important class of transcription factors (TFs); however, their roles in plant stress tolerance responses and the underlying molecular mechanisms remain largely unknown. In this study, we conducted a bioinformatic analysis of 17 members of the maize NF-YC family and examined the <em>ZmNF-YC14</em> gene through multiple sequence alignment among different species and HFD_NF-YC-like functional domains. Reverse transcription quantitative PCR (RT-qPCR) results indicated that <em>ZmNF-YC14</em> exhibited the highest expression levels in maize leaves and was positively expressed under both drought and salt stress treatments. Western blot analysis revealed a distinct band at 27.68 kDa. Analyses of <em>Escherichia coli</em> BL21 and yeast strains confirmed that <em>ZmNF-YC14</em> plays a biological role in enhancing tolerance to salt and drought stress. <em>Arabidopsis</em> plants overexpressing <em>ZmNF-YC14</em> demonstrated reduced levels of hydrogen peroxide, superoxide anion, and malondialdehyde while exhibiting increased peroxidase, catalase, and superoxide dismutase activities after drought and salt stress treatments. This effect was attributed to the reciprocal relationship between <em>ZmNF-YC14</em> and its downstream target gene <em>ZmCONSTANS-LIKE16</em>. Therefore, <em>ZmNF-YC14</em> and <em>ZmCONSTANS-LIKE16</em> may be essential for the response to abiotic stresses such as drought and salt stress in maize. They play a crucial role in the development of new germplasm, cultivation of new maize varieties, addressing the 'necklace' problem in crop breeding, and ensuring national food security.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112502"},"PeriodicalIF":4.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of Rboh enhances inorganic carbon acquisition through coordinating with carbonic anhydrase in Pyropia yezoensis","authors":"Zhizhuo Shao ,&nbsp;Menglin Guo ,&nbsp;Hong Wang ,&nbsp;Wenhui Gu ,&nbsp;Xiujun Xie ,&nbsp;Guangce Wang","doi":"10.1016/j.plantsci.2025.112497","DOIUrl":"10.1016/j.plantsci.2025.112497","url":null,"abstract":"<div><div><em>Pyropia yezoensis</em> is an important intertidal economic macroalgae, which is periodically affected by various stresses, such as the limitation of inorganic carbon (C_i) deficiency. Under such environment, the redox homeostasis within the cells of <em>P. yezoensis</em> is seriously affected, and the reactive oxygen species (ROS) signal transduction system would be activated to regulate the photosynthetic activity. Therefore, how <em>P. yezoensis</em> manage ROS to maintain effective photosynthetic carbon fixation has aroused great interest. Here, we characterize transformants overexpressing respiratory burst oxidase homolog (<em>Rboh</em>), an important gene that can actively produce ROS, at the levels of cellular physiology, biochemistry, and transcriptomics. Our data indicated the expression of <em>Rboh</em> significantly increased, accompanied by a significant upregulated expression of alpha-type carbonic anhydrase 3 (α<em>CA3</em>) and increased extracellular carbonic anhydrase activity in the <em>Rboh</em> overexpressing strains. Interestingly, compared with the wild type, the photosynthetic activity of transgenic strains was significantly higher under the low C_i and high light condition, implying that the ROS signal triggered by overexpression of <em>Rboh</em> was involved in regulating the C_i absorption and utilization in <em>P. yezoensis</em> when the C_i source was limited. In summary, this study provided evidence supporting the correlation between the ROS production and the Ci utilization under stress environments in <em>P. yezoensis</em>.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112497"},"PeriodicalIF":4.2,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription factor CsNAC25 mediating dual roles in tea plant secondary cell wall formation and trichome development","authors":"Kangli Peng ,&nbsp;Guoxiang Xiao ,&nbsp;Yin Shi ,&nbsp;Xiaozhen Huang","doi":"10.1016/j.plantsci.2025.112499","DOIUrl":"10.1016/j.plantsci.2025.112499","url":null,"abstract":"<div><div>Trichomes are a key feature of tea plants (<em>Camellia sinensis</em> L.) and essential for tea flavor compound formation, but their developmental mechanisms are still unclear. This study identified a transcription factor, <em>CsNAC25</em>, which positively regulates trichome formation in the ‘Qiancha 1’ tea plant cultivar. Phylogenetic analysis showed that <em>CsNAC25</em> shares the highest homology with <em>Arabidopsis XND1</em>, and in situ hybridization revealed its specific expression in xylem cells and in trichomes of tea plant. Overexpression of <em>CsNAC25</em> significantly inhibited xylem cell differentiation, reduced lignin and cellulose content, and led to a marked increase in trichome density. Conversely, using virus-induced gene silencing to silence <em>CsNAC25</em> in tea plants resulted in reduced trichome density and elevated lignin content. Quantitative real-time PCR analysis showed that the expression of key phenylpropanoid pathway genes, such as <em>NtPAL2</em>, <em>Nt4CL1</em>, <em>NtCAD1</em>, and <em>NtCCR1</em>, was significantly reduced in the overexpression lines. Conversely, in the <em>CsNAC25</em>-silenced tea cuttings, the expression of <em>CsPAL1</em>, <em>Cs4CL2</em>, <em>CsCAD1</em>, and <em>CsCCR1</em> was drastically increased. Moreover, the expression of <em>CsMYB1</em>, a positive regulator of trichome development, was significantly decreased in the <em>CsNAC25</em>-silenced lines. Further yeast one-hybrid and dual luciferase assays showed that <em>CsNAC25</em> binds to the <em>CsCCR1</em> promoter and represses its expression, suggesting that <em>CsNAC25</em> regulates trichome development possibly by modulating <em>CsCCR1</em> and impacting resource allocation within the phenylpropanoid metabolic network. In summary, our findings indicate that <em>CsNAC25</em> in tea plants plays a dual role in regulating the secondary cell wall formation and trichome development.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112499"},"PeriodicalIF":4.2,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrus pyrifolia WRKY31 activates the ribosomal protein gene RPL12 to confer black spot resistance","authors":"Xiangyu Cheng ,&nbsp;Caihua Xing ,&nbsp;Feng Zhang ,&nbsp;Likun Lin ,&nbsp;Keke Zhao ,&nbsp;Huizhen Dong ,&nbsp;Xiaosan Huang ,&nbsp;Shaoling Zhang","doi":"10.1016/j.plantsci.2025.112487","DOIUrl":"10.1016/j.plantsci.2025.112487","url":null,"abstract":"<div><div>Ribosomal proteins (RPs) are essential for genetic transcription and translation, playing a key role in plant growth, development, and stress responses, including disease resistance. However, the function and transcriptional regulation of RPL12 remain poorly understood. Investigating the gene function and the transcription factors that govern its expression is crucial to understanding its mechanism. In this study, a novel transcription factor gene, <em>PpWRKY31</em>, was isolated from <em>Pyrus pyrifolia</em>. The PpWRKY31 protein is expressed in the nucleus and belongs to Group IIb WRKY transcription factors. qRT-PCR analysis revealed that its expression was upregulated under the treatment of <em>Alternaria alternata</em>, as well as to exogenous hormonal treatments. Using yeast one-hybrid (Y1H) assay, dual-luciferase eporter assay, and electrophoretic mobility shift assay (EMSA), we demonstrated that PpWRKY31 can bind to the W-box element in the promoter region of <em>PpRPL12</em>. Overexpression of either <em>PpWRKY31</em> or <em>PpRPL12</em> enhanced the resistance of both pear and <em>Arabidopsis thaliana</em> plants to black spot disease, evidenced by reduced lesion size and increased activity of defense enzyme. Conversely, silencing of <em>PpWRKY31</em> or <em>PpRPL12</em> markedly diminished the resistance of pear to black spot disease. <em>PpWRKY31</em> overexpression was observed to notably enhance the expression of <em>PpRPL12</em> and genes associated with salicylic acid, inducing changes in the activity of enzymes related to the phenylpropanoid pathway, such as phenylalanine ammonia-lyase (PAL). In conclusion, this study elucidates a novel PpWRKY31-PpRPL12 signaling pathway that enhances resistance to pear black spot disease, providing insights into the regulatory networks underpinning plant defense responses.</div></div><div><h3>Core</h3><div>Pear black spot disease, caused by <em>Alternaria alternata,</em> seriously affects fruit quality and yield. We identified that <em>PpWRKY31</em> transgenic calli responded to <em>Alternaria alternata</em> in pear. PpWRKY31 binds to the W-box <em>cis</em>-element of the <em>PpRPL12</em> promoter, upregulating the expression of <em>PpRPL12</em>. The PpWRKY31-PpRPL12 regulatory module indirectly influences the downstream salicylic acid and phenylpropanoid pathways, ultimately enhancing the pear's black spot resistance.</div></div><div><h3>Gene and accession numbers</h3><div>The sequence information used in this study is available in the Pear Genome Database (<span><span>http://peargenome.njau.edu.cn/</span><svg><path></path></svg></span>), the National Center for Biotechnology Information (NCBI) database, and The Arabidopsis Information Resource, see Table S2.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112487"},"PeriodicalIF":4.2,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of grapevine BASIC PENTACYSTEINE transcription factors and functional characterization of VvBPC1 in ovule development","authors":"Songlin Zhang ,&nbsp;Haixia Zhong ,&nbsp;Fuchun Zhang ,&nbsp;Jinling Zheng ,&nbsp;Chuan Zhang ,&nbsp;Vivek Yadav ,&nbsp;Xiaoming Zhou ,&nbsp;Steve van Nocker ,&nbsp;Xinyu Wu ,&nbsp;Xiping Wang","doi":"10.1016/j.plantsci.2025.112491","DOIUrl":"10.1016/j.plantsci.2025.112491","url":null,"abstract":"<div><div>Seedless grapes are gaining increasingly attention in the market because of their desirable traits. Therefore, understanding the molecular genetic regulation of seed development and abortion is crucial for the advancement of seedless cultivars. Recent studies have shown that <em><em>AGAMOUS-LIKE11</em></em> (<em><em>VvAGL11</em></em>), an ortholog of <em><em>Arabidopsis SEEDSTICK</em></em> (<em>STK</em>), plays a key role in grape ovule development, and amino acid substitution mutations result in seed abortion. However, the regulatory pathways involved in this process are poorly understood in grapevines. In this study, we identified four <em><em>BASIC PENTACYSTEINE</em></em> (<em><em>BPC</em></em>) genes in the grapevine (<em>Vitis vinifera</em> L) genome and analyzed their evolutionary relationships, subcellular localization, and expression patterns. <em><em>VvBPC1</em></em> was identified as an upstream regulatory factor of <em><em>VvAGL11</em></em> in a yeast one-hybrid assay. Dual-luciferase assays confirmed that <em><em>VvAGL11</em></em> is negatively regulated by <em><em>VvBPC1,</em></em> and the production of small seeds by heterologous overexpression of <em><em>VvBPC1</em></em> in tomatoes results from the suppression of <em><em>VvAGL11</em></em> expression. Furthermore, assays in yeast cells demonstrated that VvBPC1 interacts with VvBELL1. Taken together, this study not only establishes the foundation for further exploration of the molecular mechanisms of the <em><em>VvBPC1-VvBELL1-VvAGL11</em></em> module in regulating grape seed development but also provides new insights into the genetic improvement of seedless grapes.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112491"},"PeriodicalIF":4.2,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cell death-inducing protein BcPlp1 from Botrytis cinerea contributes to pathogenicity and modulates plant resistance","authors":"Xiaofei Nie ,&nbsp;Ziyao Wang ,&nbsp;Binbin Huang ,&nbsp;Qiongnan Gu ,&nbsp;Ran Xu ,&nbsp;Shuang Yu ,&nbsp;Chao Xiong ,&nbsp;Zhiguo Liu ,&nbsp;Wei Wei ,&nbsp;Kai Bi ,&nbsp;Wenjun Zhu","doi":"10.1016/j.plantsci.2025.112492","DOIUrl":"10.1016/j.plantsci.2025.112492","url":null,"abstract":"<div><div><em>Botrytis cinerea</em> is a necrotrophic plant pathogen fungus with a broad host range, causing grey mould and rot diseases in many important crops, leading to significant economic losses in agriculture. Cell death-inducing proteins (CDIPs) secreted by necrotrophic phytopathogens promote plant tissue death and play important roles in infection. However, the mechanisms by which CDIPs induce cell death in <em>B. cinerea</em>-plants interactions remain unclear. Here, we demonstrate that the <em>B. cinerea</em> CDIP BcPlp1 is secreted into the plant apoplast where it induces cell death. BcPlp1 is a cysteine-rich protein, and four out of the 8 cysteine residues and a conserved N-terminal α-helix structure are essential for its cell death-inducing activity. A purified GST-tagged BcPlp1 fusion protein triggered cell death in multiple plant species, up-regulated expression of defense-related genes and enhanced plant resistance to <em>B. cinerea</em>. Additionally, the cell death-inducing activity of BcPlp1 was mediated by leucine-rich repeat (LRR) receptor-like kinases BAK1 and SOBIR1. Furthermore, <em>BcPlp1</em> was not necessary for colony morphology, conidial production, growth rate, and stress tolerance. Although deletion of <em>BcPlp1</em> did not affect virulence, its overexpression led to larger disease lesion, highlighting its contribution to <em>B. cinerea</em> pathogenicity when upregulated.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112492"},"PeriodicalIF":4.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The overexpression of DgPR1 enhances chrysanthemum cold tolerance through the ROS-mediated pathway","authors":"Yuchen Tian ,&nbsp;Yan Feng ,&nbsp;Yongyan Wang,&nbsp;Qingwu Deng,&nbsp;Xuanling Luo,&nbsp;Fan Zhang,&nbsp;Lei Zhang,&nbsp;Beibei Jiang,&nbsp;Qinglin Liu","doi":"10.1016/j.plantsci.2025.112493","DOIUrl":"10.1016/j.plantsci.2025.112493","url":null,"abstract":"<div><div>Cold stress affects the quality and yield of chrysanthemums and hinders the development of the chrysanthemum industry. PR-1, the first pathogenesis-related (PR) protein identified, is crucial in bolstering plant defenses by modulating responses to biotic and abiotic environmental pressures. Nevertheless, the precise contribution of PR-1 to chrysanthemum's resistance to cold stress is still not well defined. In the present study, a PR-1-like protein, <em>DgPR1</em>, was isolated from chrysanthemums. According to RT-qPCR analysis, <em>DgPR1</em> expression was significantly elevated in the stems compared to the roots and leaves, with a notable upregulation observed under cold stress. Both CRISPR/Cas9-facilitated gene editing and overexpression of <em>DgPR1</em> have demonstrated its beneficial effect on regulating cold-stress resistance in chrysanthemums. Overexpression of <em>DgPR1</em> in transgenic chrysanthemums enhanced cold tolerance, as evidenced by increased survival rates, reduced symptoms of low-temperature injury, and levels of hydrogen peroxide (H₂O₂), malondialdehyde (MDA), superoxide anion (O₂^-) and relative electrolyte conductivity (REC). The ROS-scavenger enzyme-activity assay showed that <em>DgPR1</em> enhanced peroxidase (POD) enzyme activity in chrysanthemums under cold stress. Additionally, the expression of <em>DgPOD</em> was higher in the overexpressing lines under cold stress compared to both the wild-type (WT) and the mutant <em>dgpr1</em>. Collectively, these findings indicate that <em>DgPR1</em> contributes positively to improving cold stress tolerance in chrysanthemums.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112493"},"PeriodicalIF":4.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}