Identification of grapevine BASIC PENTACYSTEINE transcription factors and functional characterization of VvBPC1 in ovule development

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-04-04 DOI:10.1016/j.plantsci.2025.112491

Songlin Zhang , Haixia Zhong , Fuchun Zhang , Jinling Zheng , Chuan Zhang , Vivek Yadav , Xiaoming Zhou , Steve van Nocker , Xinyu Wu , Xiping Wang

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引用次数: 0

Abstract

Seedless grapes are gaining increasingly attention in the market because of their desirable traits. Therefore, understanding the molecular genetic regulation of seed development and abortion is crucial for the advancement of seedless cultivars. Recent studies have shown that AGAMOUS-LIKE11 (VvAGL11), an ortholog of Arabidopsis SEEDSTICK (STK), plays a key role in grape ovule development, and amino acid substitution mutations result in seed abortion. However, the regulatory pathways involved in this process are poorly understood in grapevines. In this study, we identified four BASIC PENTACYSTEINE (BPC) genes in the grapevine (Vitis vinifera L) genome and analyzed their evolutionary relationships, subcellular localization, and expression patterns. VvBPC1 was identified as an upstream regulatory factor of VvAGL11 in a yeast one-hybrid assay. Dual-luciferase assays confirmed that VvAGL11 is negatively regulated by VvBPC1, and the production of small seeds by heterologous overexpression of VvBPC1 in tomatoes results from the suppression of VvAGL11 expression. Furthermore, assays in yeast cells demonstrated that VvBPC1 interacts with VvBELL1. Taken together, this study not only establishes the foundation for further exploration of the molecular mechanisms of the VvBPC1-VvBELL1-VvAGL11 module in regulating grape seed development but also provides new insights into the genetic improvement of seedless grapes.

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无籽葡萄因其理想的性状而日益受到市场的关注。因此，了解种子发育和流产的分子遗传调控对无籽栽培品种的发展至关重要。最近的研究表明，拟南芥 SEEDSTICK（STK）的直系同源物 AGAMOUS-LIKE11（VvAGL11）在葡萄胚珠发育中起着关键作用，氨基酸置换突变会导致种子流产。然而，人们对葡萄中这一过程的调控途径知之甚少。在这项研究中，我们确定了葡萄（Vitis vinifera L）基因组中的四个基本五肽（BPC）基因，并分析了它们的进化关系、亚细胞定位和表达模式。在酵母单杂交试验中，VvBPC1 被确定为 VvAGL11 的上游调控因子。双荧光素酶测定证实 VvAGL11 受 VvBPC1 的负调控，番茄中异源过表达 VvBPC1 产生的小种子是 VvAGL11 表达受抑制的结果。此外，在酵母细胞中进行的试验表明，VvBPC1 与 VvBELL1 相互作用。综上所述，本研究不仅为进一步探索 VvBPC1-VvBELL1-VvAGL11 模块调控葡萄种子发育的分子机制奠定了基础，而且为无籽葡萄的遗传改良提供了新的见解。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.