Plant Science最新文献

{"title":"Dissecting sequence-structure-function-diversity in plant cryptochromes","authors":"Pratichi Sarkar ,&nbsp;Aparna Boral ,&nbsp;Devrani Mitra","doi":"10.1016/j.plantsci.2024.112381","DOIUrl":"10.1016/j.plantsci.2024.112381","url":null,"abstract":"<div><div>Ubiquitous to every stratum of life, cryptochromes regulate numerous light dependent functions in terrestrial plants. These include light-dependent transcription, circadian rhythm, inhibition of hypocotyl elongation, programmed cell death, promotion of floral initiation, mediation of gravitropic response, responding to biotic and abiotic stress etc. There have been quite a few seminal reviews including on plant cryptochromes, focusing mostly on the detailed functional aspects. This review primarily focuses on understanding the link connecting sequence-structure hierarchy behind the functional diversity in plant cryptochromes. With available sequence information and 3D structure data, we hereby explore the molecular origin of functional diversity in both the subtypes i.e., CRY1 and CRY2. First, we discuss the structural details and functional distinctiveness of all subtypes of plant cryptochromes. Next we draw a comparison not just between two cryptochromes but also other Cryptochrome/Photolyase Family (CPF) members e.g. CRY-DASH/CRY3 and CPD/6–4 photolyases of plant origin. Further, by constructing a phylogenetic profile from multiple sequence alignment we investigate how a crucial activity like DNA repair is restricted to some members of CPF and not all. It is a well-known fact that the function of a protein is heavily if not solely guided by the structure-sequence relationship. Therefore, the resultant hypothesis as drawn from this comparative and collective study could predict functions of many under-studied plant cryptochromes when compared with their well-studied counterparts like <em>Arabidopsis</em> cryptochromes. An extensive sequence-structure-function analysis complemented with evolutionary studies and bibliographic survey is useful towards understanding the immensely diverse CPF.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112381"},"PeriodicalIF":4.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142922544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of advanced omics tools and resources for the improvement of industrial oil crops","authors":"Badal Mahakalkar ,&nbsp;Virender Kumar ,&nbsp;Sreeja Sudhakaran ,&nbsp;Vandana Thakral ,&nbsp;Sanskriti Vats ,&nbsp;Rushil Mandlik ,&nbsp;Rupesh Deshmukh ,&nbsp;Tilak Raj Sharma ,&nbsp;Humira Sonah","doi":"10.1016/j.plantsci.2024.112367","DOIUrl":"10.1016/j.plantsci.2024.112367","url":null,"abstract":"<div><div>The rapid advancement in the field of omics approaches plays a crucial role in the development of improved industrial oil crops. Industrial oil crops are important for many sectors like food processing, biofuels, cosmetics, and pharmaceuticals, making them indispensable contributors to global economies and these crops serve as vital elements in a multitude of industrial processes. Significant improvements in genomics have revolutionized the agricultural sector, particularly in the realm of oil crops. Cutting-edge advancements have facilitated the efficient sequencing of genomes for key commercial oil crops. This breakthrough not only enhances our understanding of the genetic makeup of these crops but also empowers breeders with invaluable insights for targeted genetic manipulation and breeding programs. Moreover, integrating transcriptomics with genomic data has assisted in a new era of precision agriculture. This approach provides an in-depth understanding of molecular mechanisms involved in traits of interest, such as oil content, yield potential, and resistance to biotic and abiotic stresses. Proteomics methods are instrumental in deciphering the intricacies of protein structure, interactions, and function, while metabolomics and ionomics shed light on the intricate network of metabolites and ions within biological systems. Each omics discipline offers unique insights, and their integration holds the promise of enriching our understanding and furnishing invaluable insights for enhancing oil crops. This review delves into the efficacy and constraints of various omics approaches in the context of refining industrial oil crops. Moreover, it underscores the importance of multi-omics strategies and explores their convergence with genetic engineering techniques to cultivate superior oil crop varieties.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112367"},"PeriodicalIF":4.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142922546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of the WOX gene family in Populus davidiana×P.bolleana and functional analysis of PdbWOX4 in salt resistance","authors":"Zhengyang Li ,&nbsp;Ziqian Zhang ,&nbsp;Yumeng Xu ,&nbsp;Xiaojin Lei ,&nbsp;Qinjun Xie ,&nbsp;Zhongyuan Liu ,&nbsp;Yanmin Wang ,&nbsp;Caiqiu Gao","doi":"10.1016/j.plantsci.2024.112379","DOIUrl":"10.1016/j.plantsci.2024.112379","url":null,"abstract":"<div><div>WOX transcription factors (TFs) are plant specific transcription regulatory factors that have a momentous role in maintaining plant growth and development and responding to abiotic stress. In this study, a total of 13 <em>PdbWOX</em> genes were identified. qRT-PCR analyses showed that 13 <em>PdbWOX</em> genes were responsive to salt stress. Notably, the expression of <em>PdbWOX4</em> was significantly changed at all time points under NaCl stress, suggesting that <em>PdbWOX4</em> expression may be involved in salt stress. Further, an overexpression vector of <em>PdbWOX4</em> was constructed and transient transformed into Shanxin poplar. Biochemical staining and physiological parameter analysis showed that overexpression of <em>PdbWOX4</em> decreased the total antioxidant capacity (T-AOC) and peroxidase (POD) activity, which in turn reduced the scavenging capacity of reactive oxygen species (ROS), and increased the cell damage and death induced by salt stress. qRT-PCR and ChIP-PCR demonstrated that PdbWOX4 can regulate the expression of <em>PdbDREB2C</em> by binding to its promoter. Further analyses revealed that overexpression of <em>PdbDREB2C</em> can reduce cellular damage by increasing ROS scavenging capacity thereby improving salt tolerance in Shanxin poplar. Taken together, we found that <em>PdbWOX4</em> negatively regulated the salt tolerance of Shanxin poplar by repressing the <em>PdbDREB2C</em>, suggesting that <em>PdbWOX4</em> may play a key role in the tolerance of Shanxin poplar to salt stress, and is an important candidate gene for molecular resistance breeding in forest trees.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112379"},"PeriodicalIF":4.2,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide study of the R2R3-MYB gene family and analysis of HhMYB111r-induced salt tolerance in Hibiscus hamabo Sieb. et Zucc","authors":"Yu Xu ,&nbsp;Longjie Ni ,&nbsp;Chaoguang Yu ,&nbsp;Jianfeng Hua ,&nbsp;Yunlong Yin ,&nbsp;Chunsun Gu ,&nbsp;Zhiquan Wang","doi":"10.1016/j.plantsci.2024.112378","DOIUrl":"10.1016/j.plantsci.2024.112378","url":null,"abstract":"<div><div><em>Hibiscus hamabo</em> Sieb. et Zucc. (<em>H. hamabo</em>) is a semi-mangrove plant with excellent stress tolerance that plays a crucial role in the ecological restoration of saline and alkaline areas. It is an ideal candidate species for studying the mechanisms involved in stress tolerance. Although the MYB gene family has preliminarily been characterized in <em>H. hamabo</em>, the specific functions and action mechanisms of the R2R3-MYB genes in this species have not fully been elucidated. In this study, 190 R2R3-MYB genes were identified at the genomic level using bioinformatics methods. The genes were divided into 26 subgroups based on their evolutionary relationships and found to be distributed randomly on 46 chromosomes. RNA sequencing data and subsequent real-time quantitative PCR analysis of 12 differentially expressed R2R3-HhMYB genes showed <em>HhMYB111r</em> to be highly expressed under various abiotic stress conditions. Self-activation and subcellular localization results showed that the intact HhMYB111r had strong self-activation activity and located in both the nucleus and cytoplasm. Overexpression in <em>Arabidopsis</em> significantly improved salt tolerance, and silencing <em>HhMYB111r</em> reduced the tolerance of <em>H. hamabo</em> to salt stress, indicating that <em>HhMYB111r</em> positively regulates the salt stress response. In this first analysis of the R2R3-MYB gene family in <em>H. hamabo</em>, we identified a key salt stress response gene, <em>HhMYB111r</em>, enriching the understanding of MYB function and laying a foundation for exploring the abiotic stress response of plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112378"},"PeriodicalIF":4.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new method for identifying proteins involved in DNA methylation through reverse genetics in Arabidopsis","authors":"Wei Miao ,&nbsp;Jie Dai ,&nbsp;Li Zhang ,&nbsp;Zhile Liang ,&nbsp;Xiaoxuan Sun ,&nbsp;Meizi Huang ,&nbsp;Aqin Zhang ,&nbsp;Long Zheng ,&nbsp;Yongjun Li ,&nbsp;Ying Li","doi":"10.1016/j.plantsci.2024.112376","DOIUrl":"10.1016/j.plantsci.2024.112376","url":null,"abstract":"<div><div>Forward genetic screens have uncovered numerous genes involved in DNA methylation regulation, but these methods are often time-intensive, costly, and labor-intensive. To address these limitations, this study utilized CRISPR technology to knockout selected co-expressed genes, enabling the rapid identification of low luciferase (LUC) luminescence mutants in the Col-LUC line, which harbors a LUC transgene driven by a 2 × 35S promoter in <em>Arabidopsis</em>. As proof of concept, the <em>repressor of silencing 1</em> (<em>ROS1</em>) and <em>RNA-directed DNA methylation 1</em> (<em>RDM1</em>) genes were used as controls, while the <em>increased DNA methylation 3</em> (<em>IDM3</em>) gene, co-expressed with <em>ROS1</em>, was selected as the target for gene knockout experiments. The results demonstrated that combining co-expression analysis with CRISPR technology is an effective strategy for generating low LUC luminescence mutants in the Col-LUC line. Notably, a new mutant, named <em>reduced luminescence 1</em> (<em>rl1</em>), was identified through this approach. The <em>rl1</em> mutant exhibited genome-wide DNA hypermethylation, and its reduced luminescence phenotype was largely reversed by treatment with the DNA methylation inhibitor 5-Aza-2’-deoxycytidine, confirming its anti-silencing role in DNA methylation regulation. This study presents a novel and efficient approach for obtaining low luminescence mutants in the Col-LUC line and identifies RL1 as a previously uncharacterized protein involved in DNA methylation regulation.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112376"},"PeriodicalIF":4.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of nitrate reductase in nitric oxide generation and in the induction of acclimation responses to phosphorus restriction in soybean plants","authors":"Melisa Luquet ,&nbsp;Cristian Antonelli ,&nbsp;Gustavo E. Gergoff Grozeff ,&nbsp;Agustina Buet ,&nbsp;Andrea Galatro","doi":"10.1016/j.plantsci.2024.112377","DOIUrl":"10.1016/j.plantsci.2024.112377","url":null,"abstract":"<div><div>Nitrate reductase (NR) is an essential enzyme because of its role in nitrogen metabolism and in key signaling events through the generation of the reactive nitrogen species, nitric oxide (NO). In this work, we evaluated changes in endogenous NO levels during the onset of P-restriction in soybean plants (<em>Glycine max</em>), focusing on the possible pathways involved in its generation, namely NR and NO synthase like activity, NOS_like, and the subsequent role of NR during low P-acclimation. During the first 96 h of P-starvation NO levels increased in the leaves. The treatment of plants with L-NAME (a known inhibitor of NOS) did not markedly affect NO levels, while the inhibition of NR activity with sodium tungstate (W) reduced NO-related DAF-FM fluorescence. In addition, P-restriction triggered typical acclimation responses such as the increase of acid phosphatase (AP) and ribonuclease (RNase) activities, and the accumulation of organic acids (malic and citric). Treatment of P starved plants with W reduced NR activity as well as the magnitude of the acclimation responses studied. Overall, the data here presented suggest a role for NR as NO source, influencing the signaling pathways leading to early acclimation responses following P-deprivation in soybean plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112377"},"PeriodicalIF":4.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and analysis of autophagy-related (ATG) genes in Lycium ruthenicum Murray reveals their crucial roles in salt stress tolerance","authors":"Yi Shi ,&nbsp;Yan Wu ,&nbsp;Mengling Li ,&nbsp;Na Luo ,&nbsp;Faqiang Li ,&nbsp;Shaohua Zeng ,&nbsp;Ying Wang ,&nbsp;Chao Yang","doi":"10.1016/j.plantsci.2024.112371","DOIUrl":"10.1016/j.plantsci.2024.112371","url":null,"abstract":"<div><div>Autophagy is a highly conserved intracellular degradation system that is crucial for nutrient recycling, thus regulating plant growth and development as well as in response to various stresses. Halophytic plant <em>Lycium ruthenicum</em> Murray (<em>L. ruthenicum</em>) is considered as a potential model plant for studying the physiological mechanisms of salt stress tolerance in plants. Although the genome sequence of <em>L. ruthenicum</em> is available, the characteristics and functions of the salt stress-related genes remain largely unknown. In the present study, a total of 36 AuTophaGy-related (<em>ATG</em>) genes were identified in <em>L. ruthenicum</em> and detailed characteristics of them were given. Quantitative real-time polymerase chain reaction analysis revealed that the expression of 25 <em>LrATGs</em> was significantly upregulated after salt stress treatments. Furthermore, the autophagic marker line <em>pSuper:GFP-LrATG8g</em> was generated and used to demonstrate the salt stress-induced autophagy, as revealed by measuring autophagic flux and observing autophagosome formation. The <em>pSuper:LrATG5-GFP</em> overexpression (OE) lines were also generated and further phenotypic analysis showed that OE-<em>LrATG8g</em> and OE-<em>LrATG5</em> plants exhibited better salt tolerance than that of WT plants. To the best of our knowledge, this study firstly reports a detailed overview of <em>LrATGs</em>-mediated autophagy in <em>L. ruthenicum</em> response to salt stress. These findings contribute to a global understanding of the characteristics of <em>ATG</em> genes in <em>L. ruthenicum</em> and lay a foundation for future functional study.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112371"},"PeriodicalIF":4.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ArHDZ19 contributes to drought tolerance by advancing flowering time in Anoectochilus roxburghii","authors":"Yanqin Zhu ,&nbsp;Xiaohui Zhou ,&nbsp;Jinjing Shi ,&nbsp;Bingcong Xing ,&nbsp;Ying Zheng ,&nbsp;Siqi Wan ,&nbsp;Lan ying Pan ,&nbsp;Aimin Lv ,&nbsp;Qingsong Shao","doi":"10.1016/j.plantsci.2024.112369","DOIUrl":"10.1016/j.plantsci.2024.112369","url":null,"abstract":"<div><div>The homeodomain-leucine zippper gene family encodes plant-specific transcription factors that not only affect growth and development, but also play important roles in the drought response. <em>ArHDZ19</em>, from <em>Anoectochilus roxburghii</em>, encodes a homeodomain-leucine zipper III subfamily protein whose function and molecule ar mechanism remains unclear. Here, we explored the function of ArHDZ19 in regulating growth and the drought response. ArHDZ19 localized in the nucleus and its expression was strongly induced under drought stress. Overexpression of <em>ArHDZ19</em> in <em>Arabidopsis thaliana</em> (OE-<em>ArHDZ19</em>) not only increased plant height and the length of stamens and pistils, but also resulted in an earlier flowering phenotype. The flowering-related genes <em>FLOWERING LOCUS T</em> (<em>FT</em>), <em>SUPPRESSOR OF CONSTANS OVEREXPRESSION 1</em> (<em>SOC1</em>), <em>CONSTANS</em> (<em>CO</em>), <em>FLOWERING LOCUS C</em> (<em>FLC</em>), and <em>GIGANTEA</em> (<em>GI</em>) were up-regulated in the OE-<em>ArHDZ19</em> lines. Moreover, under drought conditions, overexpression of <em>ArHDZ19</em> improved the plant survival rate and advanced the flowering time. Stress-responsive genes such as <em>COLD-REGULATED 47</em> (<em>COR47</em>), <em>KINESIN 1</em> (<em>KIN1</em>), and <em>RESPONSE TO DESICCATION (RD29A)</em> were up-regulated by drought treatment; however, their transcript levels were lower in OE-<em>ArHDZ19</em> plants than in wild-type plants. These results indicate that ArHDZ19 can improve the drought resistance of plants by advancing the flowering time, which may be a drought-avoidance mechanism.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112369"},"PeriodicalIF":4.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular ATP activates H2O2 signaling to mitigate cadmium toxicity by restricting Cd2 + entry and triggering the antioxidant system in Arabidopsis","authors":"Shurong Deng ,&nbsp;Yang Wang ,&nbsp;Chunran Huang ,&nbsp;Wei Jian ,&nbsp;Haichao Zhou ,&nbsp;Muzammil Hussain ,&nbsp;Min Pan ,&nbsp;Cheng Ye ,&nbsp;Zhengjie Zhu ,&nbsp;Tao Lang","doi":"10.1016/j.plantsci.2024.112362","DOIUrl":"10.1016/j.plantsci.2024.112362","url":null,"abstract":"<div><div>Extracellular ATP (eATP) has recently been considered important in signaling against abiotic stress in plants. However, the potential advantageous mechanisms of eATP in a plant's adaptation to cadmium (Cd) stress are largely unknown. In the present study, using eATP-insensitive mutants, <em>does not respond to nucleotides 1–3</em>/<em>4</em>, we investigated the possible roles and regulatory effects of eATP in mitigating Cd²⁺ toxicity in <em>Arabidopsis thaliana</em>. The results show that <em>dorn1–3</em> and <em>dorn1–4</em> possessed lower germination and root length, but exhibited higher relative electrolyte leakage than those in wildtype (WT) under Cd stress. In addition, CdCl₂ caused a marked trend of first increase and then decrease in eATP within the three strains during 24 h of treatment. The Cd²⁺-induced Cd²⁺ influx in the roots of <em>dorn1–3</em> and <em>dorn1–4</em> was notably higher than that in WT, whether in steady or in transient states. Additionally, the application of exogenous ATP-Na₂ (an eATP donor) reduced but exogenous PPADS (a specific inhibitor of P2K1) increased the Cd²⁺-elicited Cd²⁺ influx. The fluorescence intensities of Cd²⁺ and H₂O₂ in the mutants were also notably higher than those in WT. Furthermore, H₂O₂ signaling could be activated via eATP signaling and inhibit Cd²⁺ intry under Cd conditions. Under Cd stress, eATP-triggered H₂O₂ signaling seemed to activate the downstream transcription of genes involved in the antioxidant system, such as <em>AtGR1</em>, <em>AtCAT1</em>, <em>AtGPX8</em>, and <em>AtSOD1/2</em>, and downregulate the relative levels of <em>AtIRT1</em> and <em>AtIRT2</em> transcripts. To sum up, through binding to its receptor, P2K1, the Cd-elicited eATP potentially activated the downstream signal H₂O₂, which could further inhibit Cd entry by downregulating the expression of <em>AtIRTs</em> and remove excess ROS via upregulating genes involved in the antioxidant system, eventually leading to the mitigation of Cd toxicity.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112362"},"PeriodicalIF":4.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutation in FvPAL2 leads to light red strawberry fruits and yellow-green petioles","authors":"Chong Wang ,&nbsp;Anqi Lin ,&nbsp;Yankong Zhou ,&nbsp;Zheng Liu ,&nbsp;Peng Bai ,&nbsp;Yuxuan Zhu ,&nbsp;Junmiao Fan ,&nbsp;Xiaoyi Bi ,&nbsp;Huiyun Kuang ,&nbsp;Hongli Lian ,&nbsp;Pengbo Xu","doi":"10.1016/j.plantsci.2024.112370","DOIUrl":"10.1016/j.plantsci.2024.112370","url":null,"abstract":"<div><div>In recent years, light red or white strawberries have attracted much attention because of their unusual color, however, the mechanism of strawberry color formation, especially light red strawberry color, is not well understood. By EMS mutagenesis of woodland strawberry (<em>Fragaria vesca</em>), we identified two mutants, <em>rg40</em> and <em>rg120</em>, with light red fruit and yellow-green petiole, and allelic hybridization and BSA mixed-pool sequencing revealed that the phenotype was caused by mutation in the FvPAL2 protein in the anthocyanin synthesis pathway. Enzyme activity experiments showed that the mutant FvPAL2 protein barely catalyzed the substrate conversion normally, thus blocking anthocyanin synthesis, which in turn led to a decrease in anthocyanin accumulation in fruits and petioles. Analysis of the active pockets of the wild-type and mutant FvPAL2 proteins revealed that the mutant FvPAL2 could not bind to the substrate properly. The specific transcription factors FvMYB10 and FvMYB10L were further found to bind and activate the expression of <em>FvPAL1</em> and <em>FvPAL2</em> in both fruit and petiole. The discovery of the key site of FvPAL2 protein activity provides a clear modification target for the breeding of light red strawberry varieties, which has important application value.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112370"},"PeriodicalIF":4.2,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}