Plant Science最新文献

{"title":"The TAP-IVS-EPSPS glyphosate resistance mutation evolved in Amaranthus hybridus exhibits an adaptation cost in a glyphosate free environment","authors":"Valeria E. Perotti ,&nbsp;Valeria E. Palmieri ,&nbsp;Axl Graves ,&nbsp;Analía I. Menéndez ,&nbsp;Cecilia Casas ,&nbsp;Hugo R. Permingeat ,&nbsp;Martin M. Vila-Aiub","doi":"10.1016/j.plantsci.2025.112731","DOIUrl":"10.1016/j.plantsci.2025.112731","url":null,"abstract":"<div><div>Herbicide-resistant weeds are a prominent example of rapid global adaptation to new environments, significantly impacting the evolutionary ecology of plants, as well as the technology and economy of modern agriculture. In particular, the resistance of <em>A. hybridus</em> to glyphosate was found to be exceptionally high, and this has been recently attributed to a novel triple mutation in the <em>EPSPS</em>. Nevertheless, in environments lacking glyphosate selection pressure, it is anticipated that resistant plants would exhibit an adaptive cost, manifested as a reduction in ecological fitness relative to susceptible plants. The naturally evolved glyphosate resistance endowing triple TAP-IVS mutation in the <em>EPSPS</em> in the major weed <em>A. hybridus</em> is associated with a plant fitness penalty in environments under plant competitive interactions. This penalty is notably expressed both at the EPSPS enzyme and plant level. Plants with the TAP-IVS mutation express a notable increase in fitness cost in a competitive environment with glyphosate susceptible counterparts (i.e. ecological-based fitness cost), although this cost was absent or moderate under non-competitive conditions. Interestingly, this is also a biochemical-based fitness cost mechanism, as the EPSPS catalytic efficiency of the transformed <em>E. coli</em> TAP-IVS variant from <em>A. hybridus</em> decreased 0.3-fold compared to WT. These findings underscore the importance of considering adaptive costs when assessing the evolutionary trajectory of mutations conferring herbicide resistance.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112731"},"PeriodicalIF":4.1,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of R2R3-MYB transcription factors reveals OsMYB1 as a key regulator of anthocyanin biosynthesis in rice","authors":"Mengmeng Yin ,&nbsp;Changhe Wei ,&nbsp;Hanmei Du ,&nbsp;Tengfei Lyu ,&nbsp;Fan Luo ,&nbsp;Wenfeng Zhang ,&nbsp;Xiaoli Zhou ,&nbsp;Chengbosen Wang ,&nbsp;Lijuan Chen ,&nbsp;Dongsun Lee","doi":"10.1016/j.plantsci.2025.112732","DOIUrl":"10.1016/j.plantsci.2025.112732","url":null,"abstract":"<div><div>R2R3-MYB transcription factors (TFs) are key regulators of plant development, stress responses, and secondary metabolism, with a central role in anthocyanin biosynthesis. However, a comprehensive understanding of the R2R3-MYB TFs involved in anthocyanin accumulation in rice (<em>Oryza sativa</em> L.) remains limited. In this study, we identified 105 R2R3-MYB TFs in the rice genome and performed extensive analyses of their phylogenetic relationships, conserved motifs, gene structures, and syntenic conservation across species. Integrating phylogenetic and expression profiling data, OsMYB1—belonging to subfamily 4 (S4) and homologous to AtMYB4—emerged as a key negative regulator of anthocyanin biosynthesis. Subcellular localization analysis confirmed that OsMYB1 is localized predominantly in the nucleus. Functional assays demonstrated that OsMYB1 binds to the promoters of <em>OsDFR</em> and <em>OsANS</em>, repressing their transcription. Moreover, OsMYB1 interacts with core components of the MYB-bHLH-WD40 (MBW) activation complex, specifically OsB2 and OsPAC1, to modulate anthocyanin biosynthesis via a negative feedback mechanism. CRISPR-Cas9-mediated knockout of OsMYB1 in purple rice varieties led to a marked increase in anthocyanin accumulation in pericarps and leaves, accompanied by upregulation of key biosynthetic genes such as <em>OsF3’H</em>, <em>OsDFR</em>, and <em>OsANS</em>. These findings establish OsMYB1 as a pivotal transcriptional repressor orchestrating anthocyanin biosynthesis in rice, providing valuable insights into the regulatory networks governing flavonoid pathways. This work offers promising genetic targets for biofortification and crop improvement strategies aimed at enhancing nutritional quality and stress resilience in cereal crops.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112732"},"PeriodicalIF":4.1,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RXLR effectors from Phytophthora infestans suppress host cell death and promote virulence via nuclear localization in Nicotiana benthamiana","authors":"SangA Park ,&nbsp;Taewon Kim ,&nbsp;Soeui Lee ,&nbsp;Ye-Eun Seo ,&nbsp;Solhee In ,&nbsp;Doil Choi ,&nbsp;Joo Hyun Lee","doi":"10.1016/j.plantsci.2025.112730","DOIUrl":"10.1016/j.plantsci.2025.112730","url":null,"abstract":"<div><div><em>Phytophthora infestans</em>, a destructive hemibiotrophic pathogen, relies on effector proteins to modulate host immunity and facilitate infection. However, the molecular mechanisms by which these effectors suppress host cell death during the biotrophic phase remain poorly understood. In this study, we identified three RXLR effectors—designated as Cell Death Suppressors (CDS1: Pi02860, CDS2: Pi04089, and CDS5: Pi06099)—that strongly suppress effector-triggered cell death in <em>Nicotiana benthamiana</em>. These CDSs are highly expressed during the early biotrophic phase and inhibit cell death triggered by diverse elicitors, including INF1, NPP1.1, Rpiblb2/Avrblb2, and Avr3a/R3a. Subcellular localization analysis revealed that the nuclear targeting is essential for their cell death suppressive activity. Functional assays demonstrated that CDS1 significantly enhances <em>P. infestans</em> virulence, while CDS2 and CDS5 contribute synergistically when co-expressed. Moreover, homologs of CDS1 from other <em>Phytophthora</em> species exhibited similar suppressive activity, despite lacking obvious sequence or structural features predictive of function. Our findings suggest that <em>P. infestans</em> utilizes a coordinated set of nuclear-targeting effectors to suppress host immunity during early colonization, offering new insights into pathogen strategies for immune evasion.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112730"},"PeriodicalIF":4.1,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Connecting the dots: Role of myo-inositol oxygenase pathway in cell wall, phytic acid, and ascorbic acid biosynthesis pathways, and its potential for future crop development.","authors":"Neha Thakur ,&nbsp;Siddhant Chaturvedi ,&nbsp;Siddharth Tiwari","doi":"10.1016/j.plantsci.2025.112729","DOIUrl":"10.1016/j.plantsci.2025.112729","url":null,"abstract":"<div><div><em>Myo</em>-inositol serves as a key metabolite that contributes to the numerous biosynthetic pathways in plants. The enzyme <em>myo</em>-inositol oxygenase (MIOX) converts <em>myo</em>-inositol into D-glucuronic acid, a precursor to pectin and hemicellulose components of the plant cell wall. Previously, the role of <em>myo</em>-inositol pathway in ascorbic acid biosynthesis and its interactions with crucial biosynthetic networks such as phytic acid biosynthesis have been discussed. Nowadays, focus is increasing on enhancing the ascorbic acid content, not just to improve the nutritional quality but also to produce crops that can withstand environmental stress. The interplay between MIOX pathway, ascorbic acid, phytic acid and cell wall sugars production significantly affect the plant growth and development. Therefore, a better understanding of the interconnection between these plant pathways is crucial for the development of crop plants without any negative developmental effects. The current study addresses the knowledge gaps and present a particular perspective on the relation of MIOX pathway with cell wall, phytic acid and ascorbic acid biosynthesis pathways in plants. Additionally, it also provides information on MIOX pathway genes that have shown potential in providing tolerance to abiotic stresses (such as heat, cold, drought and salt) in plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112729"},"PeriodicalIF":4.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-associated mechanisms in plant responses to abiotic stress","authors":"Faamiya Shajar ,&nbsp;Azha Ufaq Nabi ,&nbsp;Amina Manzoor ,&nbsp;Seerat Saleem ,&nbsp;Naveed Ul Mushtaq ,&nbsp;Shaista Manzoor ,&nbsp;Reiaz Ul Rehman","doi":"10.1016/j.plantsci.2025.112726","DOIUrl":"10.1016/j.plantsci.2025.112726","url":null,"abstract":"<div><div>The plasma membrane (PM) is a versatile and complex biological membrane, consisting of a dynamic assembly of lipids and proteins that protects the cell and separates it from its external environment. Lipids are essential in preserving the stability of the membrane while also regulating the activity of membrane proteins. Additionally, the PM significantly contributes to detecting environmental stress and serves as the primary target of its effects. These stressors induce modifications in the membrane composition, structure, and properties, which can ultimately disrupt cellular homeostasis. Ensuring the integrity and fluidity of the PM is vital for plants to survive harsh environmental conditions. The ability of plants to alter the composition of membrane lipids and proteins is vital for their adaptation to a range of abiotic stresses, such as extreme temperatures, drought, salinity, and heavy metal (HM) toxicity. Lipid remodelling influences the activity of membrane transporters which eventually governs the physical characteristics of the PM. To cope with harsh environment, plants rely on membrane transport systems working in tandem with the lipidome to uphold membrane stability. The review offers a comprehensive overview of the essential functions of PM proteins and lipids during stress, emphasizing the signalling pathways activated by the PM to counteract abiotic stress.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112726"},"PeriodicalIF":4.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcrossing preference in Magnolia ×soulangeana 'Hongyun' is associated with differential stigmatic ROS accumulation regulated by MsFERONIA–MsROP2–MsROBHD module","authors":"Liyong Sun ,&nbsp;Qingxian Wu ,&nbsp;Yao Chen ,&nbsp;Lina Xu ,&nbsp;Shuxian Li ,&nbsp;Zengfang Yin","doi":"10.1016/j.plantsci.2025.112724","DOIUrl":"10.1016/j.plantsci.2025.112724","url":null,"abstract":"<div><div>As primitive angiosperms, bisexual <em>Magnolia</em> species evolved a relatively advanced mating system, namely facultative outcrossing, avoiding inbreeding depression meanwhile providing a certain reproductive assurance. Explosive advances have been made in the molecular understanding of pollen<em>–</em>pistil interactions in the past decades, especially emphasizing the role of <em>FERONIA–RAC/ROP–RBOHD</em> module. However, relevant molecular framework in primitive angiosperms remains largely unknown. In this study, our systematic investigation showed that outcrossing rather than selfing benefited pollen hydration and pollen tube growth via rapidly inhibiting stigmatic ROS accumulation in <em>Magnolia ×soulangeana</em> 'Hongyun'. It was attributed to the differential expression and regulation of the <em>MsFERONIA–MsROP2–MsRBOHD</em> module. Particularly, outcrossing strongly inhibited the expression of <em>MsRBOHD</em> throughout the pollen<em>–</em>pistil interaction. Gene function verification showed that direct overexpression of <em>MsFERONIA</em> conferred a rapid stigmatic ROS response and promoted compatible pollen growth in <em>Arabidopsis</em>. Moreover, <em>MsRBOHD</em> overexpression resulted in elevated stigmatic ROS, suppressing compatible pollen growth in <em>Arabidopsis</em>. Overall, these results emphasized that this conserved <em>MsFERONIA–MsROP2–MsRBOHD</em> module played a pivotal role in the regulation of pollen<em>–</em>pistil interactions via mediating stigmatic ROS in 'Hongyun'. Our findings could deepen understanding of the molecular basis underlying mate selection in primitive angiosperms, and provide novel insights into breeding and conservation of endangered rare species.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112724"},"PeriodicalIF":4.1,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144885556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Partial disruption of the Arabidopsis 2S seed storage proteins highlights the essential role of redox homeostasis in seed proteome plasticity and rebalancing","authors":"Clement Bagaza ,&nbsp;Huda Ansaf ,&nbsp;Abou Yobi ,&nbsp;Kirk Czymmek ,&nbsp;Thomas P. Mawhinney ,&nbsp;Amanda Agosto Ramos ,&nbsp;Daniel J. Kliebenstein ,&nbsp;Dan H. Cohen ,&nbsp;Hagai Yasuor ,&nbsp;Ruthie Angelovici","doi":"10.1016/j.plantsci.2025.112714","DOIUrl":"10.1016/j.plantsci.2025.112714","url":null,"abstract":"<div><div><em>Arabidopsis</em> seeds store most of their amino acids in the 12S and 2S seed storage proteins (SSPs). Elimination of the three most abundant 12S cruciferins (i.e., CRUA, CRUB, and CRUC) led to broad translational adjustments and redox homeostasis alteration. However, it remains unclear whether these responses are specific to major SSPs perturbation or represent core processes essential for proteome plasticity during seed filling, even if the perturbation is limited in scope. To address this question, we investigated the effects of disrupting the second most abundant SSPs, the 2S seed storage albumin (SESA) or napins. We performed physiological and proteomic analyses on a <em>napin-RNAi</em> mutant, which revealed that disruption of napins primarily affects oxidative homeostasis, with limited translational adjustment. These findings suggest that redox homeostasis is a fundamental response to proteomic perturbation during seed filling, whereas translational adjustments appear more contingent on the severity of disruption, particularly when large-scale compensatory protein response is required. Germination assays performed on cruciferin (<em>cruabc)</em> and napin (<em>napin-RNAi</em>) mutants highlighted the impact of redox homeostasis on seed germination. Notably, both mutants exhibited delayed germination, which was alleviated by supplementation with reactive oxygen species (ROS)-inducing agents. Importantly, analysis of the differentially expressed proteins that overlap between the two mutants identified a core set of proteins involved in proteome rebalancing. These shared targets represent promising candidates for future functional characterization and potential avenues for seed biofortification.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112714"},"PeriodicalIF":4.1,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144885557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A real-time visualized TRSV-based gene silencing method using trichome as a selected marker in cucumber","authors":"Xu Wang ,&nbsp;Chenhao Zhou ,&nbsp;Xiaoli Gao ,&nbsp;Jia Luo ,&nbsp;Yong He ,&nbsp;Lihao Wang ,&nbsp;Zhi Xu ,&nbsp;Zhujun Zhu ,&nbsp;Yunmin Xu","doi":"10.1016/j.plantsci.2025.112728","DOIUrl":"10.1016/j.plantsci.2025.112728","url":null,"abstract":"<div><div>Cucumber (<em>Cucumis sativus</em>. L) is economically valuable vegetable crop worldwide. Although cucumber genomic sequence has been completed, the functions of most genes have not yet been characterized. Virus-induced gene silencing (VIGS) is an efficient system for investigating gene function in plants, however, detection of VIGS efficiency by PCR is a time-consuming method. In this study, a vacuum-agroinfiltrated Tobacco ringspot virus (TRSV)-based gene silencing method was developed in cucumber, and <em>CsGLABROUS3</em> (<em>CsGL3</em>), which functions in initiation of trichome, was cloned into <em>pTRSV2</em> vector to develop a TRSV-CsGL3 system. Gene silenced cucumbers were visible using trichome as a selected marker, and their glabrous phenotype exhibited throughout the life cycle in TRSV-CsGL3 system. Thereafter, a flower morphogenesis gene (<em>UNUSUAL FLORAL ORGANS</em>, <em>CsUFO</em>) was selected to silence by the TRSV-CsGL3 system, and <em>CsUFO</em> silenced cucumbers produced the flower defect phenotype as expected. In summary, TRSV-CsGL3 is a real-time visualized VIGS system using trichome as a selected marker, which simplify the VIGS identification procedure, and it can be used to investigate gene function throughout the life cycle in cucumber.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112728"},"PeriodicalIF":4.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the full-length GbERD7 gene family in Gossypium barbadense and functional analysis of the role of the GbERD7g gene in drought and salt tolerance","authors":"Zheng Zong ,&nbsp;Xue Sun ,&nbsp;Junchen Chen ,&nbsp;Yuehua Yu ,&nbsp;Zhiyong Ni ,&nbsp;Yi Wang","doi":"10.1016/j.plantsci.2025.112715","DOIUrl":"10.1016/j.plantsci.2025.112715","url":null,"abstract":"<div><div><em>ERD</em> (early response to dehydration) genes are promptly upregulated under dehydration stress and are pivotal in plant development. Nonetheless, the precise impact of the <em>ERD7</em> gene on the response of cotton to abiotic stress remains unclear. The physical and chemical characteristics, gene architecture, gene collinearity, and transcriptomic profiles were examined. Using bioinformatics techniques, we investigated the evolutionary relationships among the genes within the <em>GbERD7</em> gene family of sea island cotton. The <em>GbERD7</em> genes are unevenly distributed across the seven chromosomes of sea island cotton, with multiple gene duplications. The <em>GbERD7</em> gene family was subjected to phylogenetic analysis, leading to the classification of its members into the SENA and SENB subfamilies. The expression of the <em>GbERD7</em> genes was investigated in relation to heat, low-temperature, salt (NaCl), and polyethylene glycol (PEG) treatments. Some genes presented greater expression in specific organs and different periods of fiber development. The functional role of <em>GbERD7g</em> was subsequently investigated using molecular biological techniques. <em>GbERD7g</em> exhibited pronounced expression in sea island cotton leaves and was upregulated following exposure to PEG, NaCl, and ABA. Subcellular localization studies revealed that the GbERD7g protein is located within the nucleus as well as the plasma membrane of the cell. When the <em>GbERD7g</em> gene was silenced under drought and salt stress, the sea island cotton plants were significantly less resistant to drought and salinity and exhibited lower survival than the control plants. The proline levels, catalase activity, and superoxide dismutase activity were reduced, and the malondialdehyde and hydrogen peroxide levels were elevated. In addition, compared with those in the control plants, the expression of all three stress-responsive genes, namely, <em>GbRD22</em>, <em>GbRD26</em>, and <em>GbCDPK1</em>, was significantly lower in the mutant plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112715"},"PeriodicalIF":4.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How plants respond to salt stress: Lessons form RBOHs","authors":"MengYing Sun ,&nbsp;Xiang Liu ,&nbsp;Han Zheng ,&nbsp;Ling Li ,&nbsp;QiMeng Lv ,&nbsp;GaoPeng Wang","doi":"10.1016/j.plantsci.2025.112710","DOIUrl":"10.1016/j.plantsci.2025.112710","url":null,"abstract":"<div><div>RBOH is called respiratory burst oxidase homolog is one of the key enzymes in producing reactive oxygen species (ROS). Studies have shown that ROS (such as ·O₂^-, H₂O₂) are widely involved in plant growth development and response to various biological and abiotic stresses. In recent years, with the gradual deepening of research, more and more plant NOX/RBOH members have been identified, and their biological functions and participating signal regulatory pathways have been revealed. The purpose of this review is to summarize the whole pathway that the expression of one or more genes upstream and downstream of RBOH proteins directly or indirectly stimulates RBOH proteins to regulate ROS outburst, ROS clearance and inter-cellular transfer. The structure and function, expression regulation, ROS signaling and clearance of RBOH family were discussed in order to provide reference for further study and utilization of RBOH family in plant response to abiotic stress.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"360 ","pages":"Article 112710"},"PeriodicalIF":4.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}