Plant Science最新文献

{"title":"Loss of PII-dependent control of arginine biosynthesis in Dunaliella salina","authors":"Vitalina Vlasova ,&nbsp;Tatiana Lapina ,&nbsp;Qi Cheng ,&nbsp;Elena Ermilova","doi":"10.1016/j.plantsci.2024.112327","DOIUrl":"10.1016/j.plantsci.2024.112327","url":null,"abstract":"<div><div>In cyanobacteria and most Archaeplastida, Arg regulates its formation via allosteric inhibition of the controlling enzyme, N-acetyl-L-glutamate kinase (NAGK) that requires PII protein to properly sense the feedback inhibitor. Although PII expression has been shown to be reduced in <em>Dunaliella salina</em> compared to other green algae, the potential impact of this protein on DsNAGK activity remains unclear. We here performed coupled enzyme assay and surface plasmon resonance analysis and show that DsNAGK is activated by NAG and inhibited by Arg but is not controlled by DsPII. Moreover, DsPII has likely lost its function as an effective glutamine sensor. Replacement of the C-terminus from DsPII with the C-terminus from <em>Chlamydomonas</em> PII restored sensitivity to glutamine in a recombinant DsPII protein, demonstrating the importance of C-terminal residues close to the Q-loop for PII functions. The findings are discussed in the context of the relationship between NAGK control and the acquisition of salinity tolerance during evolution.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"351 ","pages":"Article 112327"},"PeriodicalIF":4.2,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of pecan PEBP family genes and the potential regulation role of CiPEBP-like1 in fatty acid synthesis","authors":"Jiani Wang ,&nbsp;Xinyao Liu ,&nbsp;Qiaoyan Wang,&nbsp;Miao Shi,&nbsp;Caiyun Li,&nbsp;Huating Hou,&nbsp;Kean-Jin Lim,&nbsp;Zhengjia Wang,&nbsp;Zhengfu Yang","doi":"10.1016/j.plantsci.2024.112326","DOIUrl":"10.1016/j.plantsci.2024.112326","url":null,"abstract":"<div><div>Phosphatidyl ethanolamine-binding protein (PEBP) plays important roles in plant growth and development. However, few studies have investigated the PEBP gene family in pecan (<em>Carya illinoinensis</em>), particularly the function of the PEBP-like subfamily. In this study, we identified 12 PEBP genes from the pecan genome and classified them into four subfamilies: MFT-like, FT-like, TFL1-like and PEBP-like. Multiple sequence alignment, gene structure, and conserved motif analyses indicated that pecan PEBP subfamily genes were highly conserved. <em>Cis</em>-element analysis revealed that many light responsive elements and plant hormone-responsive elements are found in CiPEBPs promoters. Additionally, RNA-seq and RT-qPCR showed that <em>CiPEBP-like1</em> was highly expressed during kernel filling stage. GO and KEGG enrichment analysis further indicated that <em>CiPEBP-like1</em> was involved in fatty acid biosynthesis and metabolism progress. Overexpression of <em>CiPEBP-like1</em> led to earlier flowering and altered fatty acid composition in <em>Arabidopsis</em> seeds. RT-qPCR confirmed that <em>CiPEBP-like1</em> promoted fatty acid synthesis by regulating the expression of key genes. Overall, this study contributes to a comprehensive understanding of the potential functions of the PEBP family genes and lay a foundation to modifying fatty acid composition in pecan kernel.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"351 ","pages":"Article 112326"},"PeriodicalIF":4.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PyWRKY40 negatively regulates anthocyanin synthesis in pear fruit","authors":"Xuefeng Zhang ,&nbsp;He Zhang ,&nbsp;Mingxin Yin,&nbsp;Siyang Gao,&nbsp;Mingyang Xu,&nbsp;Guodong Du","doi":"10.1016/j.plantsci.2024.112323","DOIUrl":"10.1016/j.plantsci.2024.112323","url":null,"abstract":"<div><div>The deposition of anthocyanin plays a crucial role in fruit pigmentation and serves as the primary determinant of pear quality. Various factors influence the synthesis of anthocyanins, with salicylic acid playing a significant role among them. However, the mechanism by which salicylic acid affects anthocyanin synthesis remains unclear. Our study identifies the transcription factor PyWRKY40 as a pivotal regulator of SA-mediated anthocyanin synthesis in the nucleus. The negative regulatory function of this factor lies in its ability to suppress anthocyanin synthesis, thereby exerting an influence on fruit coloration. We have confirmed the direct binding of PyWRKY40 to the <em>PyDFR</em> promoter through Y1H and EMSA experiments. The findings elucidate a signaling regulatory module, PyWRKY40-PyDFR, which is responsive to SA and enhances fruit pigmentation by modulating anthocyanin metabolism. This insight offers a viable approach to enhancing fruit coloration and improving the overall quality of pear fruits.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"351 ","pages":"Article 112323"},"PeriodicalIF":4.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increase of histone acetylation by suberoylanilide hydroxamic acid enhances microspore reprogramming and expression of somatic embryogenesis transcription factors in Brassica napus","authors":"Yolanda Pérez-Pérez ,&nbsp;Eduardo Berenguer ,&nbsp;Elena Carneros ,&nbsp;Pilar S. Testillano","doi":"10.1016/j.plantsci.2024.112318","DOIUrl":"10.1016/j.plantsci.2024.112318","url":null,"abstract":"<div><div><em>In vivo</em>, microspores in the anthers follow the gametophytic development pathway, culminating in the formation of pollen grains. Conversely, <em>in vitro</em>, under stress treatments, microspores can be reprogrammed into totipotent cells, initiating an embryogenic pathway that produces haploid and double-haploid embryos, which are important biotechnological tools in plant breeding. There is growing evidence that epigenetic reprogramming occurs during microspore embryogenesis through DNA methylation, but less is known about the role of histone modifications. This study investigates the dynamics of histone acetylation during the two microspore developmental pathways, microspore embryogenesis and pollen development, in <em>Brassica napus</em>. We analyzed histone H3 and H4 acetylation levels, histone acetyltransferase (HAT) activity and expression of <em>HAC5</em> acetyltransferase using immunofluorescence, enzymatic activity assays, ELISA-like tests, and qPCR. Results showed a decrease in global histone acetylation levels during pollen maturation, correlated with reduced HAT activity and downregulation of the <em>BnHAC5-like</em> gene. In contrast, stress-induced microspore reprogramming led to increased histone acetylation levels, enhanced HAT activity, and upregulation of <em>BnHAC5-like</em>. Treatment with suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor widely used in animal research but barely applied to plants, enhanced microspore embryogenesis initiation and proembryo formation, while increased histone acetylation levels. SAHA-treated proembryos showed higher expression than control of key embryogenesis transcription factors <em>BnFUS3</em>, <em>BnAGL15</em>, and <em>BnLEC2</em>, suggesting that histone hyperacetylation facilitates transcriptional activation of essential genes for somatic embryogenesis initiation. These findings provided new insights into the epigenetic regulation of this process and revealed new opportunities with histone epigenetic regulator inhibitors, to improve microspore embryogenesis induction for crop improvement.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"351 ","pages":"Article 112318"},"PeriodicalIF":4.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and functional characterization of volatile-terpene synthase genes from Chamaecyparis obtusa var. formosana","authors":"Ting-Wei Chen ,&nbsp;Nai-Wen Tsao ,&nbsp;Sheng-Yang Wang ,&nbsp;Fang-Hua Chu","doi":"10.1016/j.plantsci.2024.112322","DOIUrl":"10.1016/j.plantsci.2024.112322","url":null,"abstract":"<div><div><em>Chamaecyparis obtusa</em> var. <em>formosana</em> is significant as a precious and endemic plant in Taiwan. The trunk, renowned for its excellent texture and color, is ideal for construction materials and furniture. Moreover, the entire plant is rich in aroma, which can be made into essential oils, fragrances, and a series of related products. Volatile terpenoids are the major compounds in the composition of essential oils, many of which can only be found in <em>C. obtusa</em> var. <em>formosana</em>. In this study, we successfully identified 12 volatile terpene synthases from <em>C</em>. <em>obtusa</em> var. <em>formosan</em>a. Most of the selected TPSs displayed the ability to catalyze precursors into cyclic terpenoids, except for CovfTPS8, which also exhibited the capability to react with FPP and GPP. CovfTPS10 is particularly noteworthy for its multi-product characteristics and the ability to synthesize acoradiene. Moreover, it produces a novel compound, cis-isoduacene. Through the investigation of these volatile-terpenoid synthases, we can gain a better understanding of the cyclization process for terpenoids.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"350 ","pages":"Article 112322"},"PeriodicalIF":4.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SlTDF1: A key regulator of tapetum degradation and pollen development in tomato","authors":"Zhengliang Sun ,&nbsp;Baohui Cheng ,&nbsp;Yanhong Zhang ,&nbsp;Liangzhe Meng ,&nbsp;Yuhe Yao ,&nbsp;Yan Liang","doi":"10.1016/j.plantsci.2024.112321","DOIUrl":"10.1016/j.plantsci.2024.112321","url":null,"abstract":"<div><div>Pollen formation and development during the life cycle of flowering plant are crucial for maintaining reproductive and genetic diversity. In this study, an R2R3MYB family transcription factor, <em>SlTDF1</em> (<em>SlMYB35</em>), was predominantly expressed in stamens. Repressed expression of <em>SlTDF1</em> results in a delay in the degradation of the anther tapetum in tomatoes, which in turn leads to the formation of abnormal pollen, including a reduction in the number of single-fruit seeds and fertility when compared to wild-type plants. Analysis of paraffin sections demonstrated that SlTDF1 is a crucial factor in the maturation of tomato pollen. Further analysis of the transcriptomic data revealed that downregulation of the <em>SlTDF1</em> gene significantly suppressed the expression of genes related to sugar metabolism and anther development. The findings of this study indicated that <em>SlTDF1</em> plays a pivotal role in regulating tomato pollen development. Moreover, these findings provide a genetic resource for male sterility in tomato plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"351 ","pages":"Article 112321"},"PeriodicalIF":4.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intragenic cytosine methylation modification regulates the response of SUCLα1 to lower temperature in Solanaceae","authors":"Cuihua Xin ,&nbsp;Junjie Wang ,&nbsp;Junling Chi ,&nbsp;Yang Xu ,&nbsp;Ruiping Liang ,&nbsp;Lei Jian ,&nbsp;Liangming Wang ,&nbsp;Jiangbo Guo","doi":"10.1016/j.plantsci.2024.112320","DOIUrl":"10.1016/j.plantsci.2024.112320","url":null,"abstract":"<div><div>The tricarboxylic acid cycle (TCAC) is a fundamental metabolic process governing matter and energy in plant cells, playing an indispensable role. However, its involvement in responding to low temperature stress in potato remains poorly understood. Previous studies have identified succinyl-CoA ligase (SUCL), which catalyzes the phosphorylation of TCAC substrates, as a gene associated with lower temperatures. Nevertheless, its function in potato's response to lower temperatures remains unclear. Phylogenetic analysis has revealed that <em>Solanum tuberosum</em> possesses α and β subunits of SUCL, which cluster with those of <em>Solanum lycopersicum</em>, <em>Nicotiana tabacum</em> and <em>Nicotiana benthamiana</em>. Further investigation has shown that StSUCLα1 is predominantly located within mitochondria. Low temperatures induce methylation modification alterations at 11 intragenic cytosine sites and lead to changes in <em>StSUCLα1</em> expression levels. Correlation analysis suggests that alterations in intragenic cytosine methylation sites of <em>SUCLα1</em> may be associated with MET1. Knocking down <em>NbSUCLα1</em>, the homologous gene of <em>StSUCLα1</em> in <em>N. benthamiana</em>, results in increased susceptibility to low temperature stress in plants. In summary, we have confirmed that <em>SUCLα1</em> is a key gene modulated by intragenic cytosine methylation in response to lower temperatures, providing a novel target for genetic breeding aimed at enhancing potato tolerance to low temperature stress.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"350 ","pages":"Article 112320"},"PeriodicalIF":4.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The PHD transcription factor ThPHD5 regulates antioxidant enzyme activity to increase salt tolerance in Tamarix hispida","authors":"Yao-Shuo Tan ,&nbsp;Jing-Hang Li ,&nbsp;Pei-Long Wang ,&nbsp;Dan-Ni Wang ,&nbsp;Bai-Chao Liu ,&nbsp;Sonethavy Phetmany ,&nbsp;Yong-Xi Li ,&nbsp;Qing-Jun Xie ,&nbsp;Cai-Qiu Gao","doi":"10.1016/j.plantsci.2024.112319","DOIUrl":"10.1016/j.plantsci.2024.112319","url":null,"abstract":"<div><div>PHD proteins are an important class of transcription factors (TFs) that are widely distributed in eukaryotes and play crucial roles in many aspects of plant growth, development and response to stress. We identified a transcription factor, ThPHD5, from the PHD family in <em>Tamarix hispida</em> based on its potential involvement in abiotic stress response processes. In this study, the salt tolerance function of <em>ThPHD5</em> from <em>T</em>. <em>hispida</em> was further characterized. The qRT-PCR results showed <em>ThPHD5</em> expression was significantly induced by NaCl, PEG and ABA treatments. Transient transformation analysis revealed that <em>ThPHD5</em> improved salt tolerance in <em>T</em>. <em>hispida</em> by increasing POD and SOD activity, decreasing the MDA, total ROS content and electrolyte leakage. To explore the salt tolerance mechanism of the ThPHD5 TF, its binding DNA motifs and potential downstream regulatory genes were analyzed. The results showed that <em>ThPHD5</em> affect the expression of 7 antioxidant enzyme-related genes. The Yeast one-hybrid (Y1H) and Electrophoretic Mobility Shift Assay (EMSA) results indicated <em>ThPHD5</em> could bind to ABRE, MYB and Dof cis-acting elements. ChIP-PCR further confirmed <em>ThPHD5</em> exercise its regulatory function by directly binding motifs on the <em>ThPOD16</em>, <em>ThSOD</em> and <em>ThSOD1</em> promoters. Taken together, these findings indicate the ThPHD5 TF improves salt tolerance in <em>T</em>. <em>hispida</em> by regulating the expression of antioxidant enzyme-related genes to increase antioxidant enzyme activity, enhance the ROS scavenge ability, reduce ROS accumulation and cellular damage.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"350 ","pages":"Article 112319"},"PeriodicalIF":4.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analyses at specific plastochrons reveal timing and involvement of phytosulfokine in maize vegetative phase change","authors":"Krista Osadchuk,&nbsp;Ben Beydler,&nbsp;Chi-Lien Cheng,&nbsp;Erin Irish","doi":"10.1016/j.plantsci.2024.112317","DOIUrl":"10.1016/j.plantsci.2024.112317","url":null,"abstract":"<div><div>Successive developmental stages of representative early and late juvenile, transition, and adult maize leaves were compared using machine-learning-aided analyses of gene expression patterns to characterize vegetative phase change (VPC), including identification of the timing of this developmental transition in maize. We used t-SNE to organize 32 leaf samples into 9 groups with similar patterns of gene expression. oposSOM yielded clusters of co-expressed genes from key developmental stages. TO-GCN supported a sequence of events in maize in which germination-associated ROS triggers a JA response, both relieving oxidative stress and inducing miR156 production, which in turn spurs juvenility. Patterns of expression of <em>MIR395</em>, which regulates sulfur assimilation, led to the hypothesis that phytosulfokine, a sulfated peptide, is involved in the transition to adult patterns of differentiation.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"350 ","pages":"Article 112317"},"PeriodicalIF":4.2,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tree biology: From genomics to genetic improvement","authors":"Jinbo Shen,&nbsp;Wan-Feng Li,&nbsp;Chang Liu,&nbsp;Shuai Zheng","doi":"10.1016/j.plantsci.2024.112315","DOIUrl":"10.1016/j.plantsci.2024.112315","url":null,"abstract":"","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"350 ","pages":"Article 112315"},"PeriodicalIF":4.2,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}