Potassium uptake function of LbKT1 and LbSKOR from Lycium barbarum and their influence on the arbuscular mycorrhizal symbiosis

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-05-31 DOI:10.1016/j.plantsci.2025.112587

Xia Han , Yuhao Zhou , Xiaoxue Feng , Yihan Wang , Haoqiang Zhang

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Abstract

Potassium participates in a variety of plant physiological processes and has great impact on plant growth and stress adaptation. The absorption of potassium by Plant is mediated by potassium channels and transporters, and the Shaker potassium channel gene family plays an important role in potassium uptake. Arbuscular mycorrhizal (AM) fungi form ubiquitous symbioses with plants and increase plants’ potassium uptake. However, few studies have focused on the interaction of plant potassium channels from the Shaker gene family with AM fungi. In this study, the potassium uptake function of LbKT1 and LbSKOR (homologs of AKT1 and SKOR in Arabidopsis) from the Shaker gene family in Lycium barbarum was verified by the complementary assay using a yeast potassium uptake mutant. LbKT1 and LbSKOR were also overexpressed in tobacco to assess their influence on AM fungi under low and normal potassium conditions in a pot experiment. LbKT1 could rescue the phenotype of the yeast mutant, while LbSKOR could not. Overexpression of LbKT1 increased tobacco plant growth and potassium uptake and promoted the colonization of AM fungi. Meanwhile, overexpression of LbSKOR promoted potassium translocation from root to shoot and showed no obvious influence on the colonization of AM fungi. Our results suggested that the AM fungi could promote tobacco growth and potassium uptake, while the plant potassium status and the AM fungal colonization may form positive feedback in promoting tobacco potassium uptake and growth.

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枸杞LbKT1和LbSKOR的钾吸收功能及其对丛枝菌根共生的影响

钾参与多种植物生理过程，对植物生长和逆境适应具有重要影响。植物对钾的吸收是由钾通道和转运体介导的，其中Shaker钾通道基因家族在钾吸收中起着重要作用。丛枝菌根（AM）真菌与植物形成无所不在的共生关系，增加植物对钾的吸收。然而，很少有研究关注Shaker基因家族植物钾通道与AM真菌的相互作用。本研究利用酵母钾摄取突变体，通过互补实验验证了枸杞中Shaker基因家族的LbKT1和LbSKOR（拟南芥中AKT1和SKOR的同源物）的钾摄取功能。在盆栽试验中，LbKT1和LbSKOR也在烟草中过表达，以评估它们在低钾和正常钾条件下对AM真菌的影响。LbKT1可以挽救酵母突变体的表型，而LbSKOR则不能。过表达LbKT1促进烟草植株生长和钾吸收，促进AM真菌定植。同时，过表达LbSKOR促进了钾从根向茎的转运，对AM真菌的定植无明显影响。结果表明，AM真菌可以促进烟草生长和钾吸收，植株钾状态与AM真菌定殖在促进烟草钾吸收和生长方面可能形成正反馈。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.