SeXTH23通过调节杨树多肉化和渗透调节来增强其耐盐性。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-09-24 DOI:10.1016/j.plantsci.2025.112783

Huirong Duan , Richard John Tiika , Guangxin Cui , Fuping Tian , Yu Hu , Xinqiang Zhu , Yuan Lu , Qian Zhang , Chunmei Wang , Yi Li , Hongshan Yang

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引用次数: 0

摘要

盐角藓在高盐度环境下的生长能力显著，是研究盐适应的重要盐生植物模型。在本研究中，50-300mM NaCl处理能促进黑麦生长，增加组织肉质和水分含量，促进离子的积累和分配。SeXTH23含有一个保守的催化结构域和分泌信号肽，在根和维管组织中高表达，表明其在逆境中细胞壁重塑中的潜在作用。过表达SeXTH23的转基因杨树杂种通过提高多肉质、含水量、脯氨酸积累和光合效率来提高耐盐性，同时降低氧化损伤（降低MDA）和失水率。SeXTH23- rnai S. europaea表现出DW减少、多肉质减少和失水迅速。这些结果为将SeXTH23作为工程耐盐作物的遗传靶点应用奠定了基础。

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SeXTH23 enhances salt tolerance by regulating succulence and osmotic adjustment in aspen hybrid

Salicornia europaea serves as a key halophyte model for studying salt adaptation due to its remarkable ability to thrive under high salinity. In the present study, 50–300 mM NaCl enhanced S. europaea growth, accompanied with increased tissue succulence and water content, also effective ion accumulation and distribution. SeXTH23, harboring a conserved catalytic domain and secretory signal peptide, was highly expressed in shoots and vascular tissues, indicating its potential role in vascular cell wall remodeling during stress. Overexpression of SeXTH23 in transgenic aspen hybrid improved salt tolerance by enhancing succulence, water content, proline accumulation and photosynthetic efficiency, while reducing oxidative damage (lower MDA) and water loss rate. SeXTH23-RNAi S. europaea plants exhibited reduced dry weight, succulence, and rapid water lost. These results form a foundation for the application of SeXTH23 as genetic target for engineering salt-tolerant crops.

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.