Glutathione metabolism and free amino acid profile in Amaranthus palmeri with non-target site-based resistance to sulfonylureas

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-09-20 DOI:10.1016/j.plantsci.2025.112781

Mikel V. Eceiza , Miriam Gil-Monreal , Clara Jimenez-Martinez , Laura Estepa , Eneko Trebol-Aizpurua , Mithila Jugulam , Mercedes Royuela , Ana Zabalza

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引用次数: 0

Abstract

Although the majority of Amaranthus palmeri populations resistant to ALS inhibitors are due to alterations in the enzyme (target-site resistance mechanism), there are some cases of resistance due to herbicide metabolization (which is a non-target site (NTS) mechanism). In this study, we investigated the physiology of a sensitive (S) population and a sulfonylurea-resistant population due to NTSR of A. palmeri (R). Both populations were grown hydroponically and treated with the ALS inhibitor nicosulfuron, alone or combined with NBD-Cl (GST inhibitor). Seven days later, free amino acid profile, glutathione (GSH) content and GST activity and expression were measured in the leaves. Only S population leaves showed the typical amino acid profile previously described after sulfonylureas; R population showed no change, suggesting that such physiological disruption is prevented as ALS activity is maintained through herbicide metabolism. In the S population, the herbicide-induced oxidative stress triggered an increase in GST activity and accumulation of glutathione-related compounds (GSH and its precursors). R population showed a higher basal GST activity than S population and GST activity was induced by the herbicide. Three Phy and three Tau GSTs were evaluated, but none were clearly linked to increased activity. Root length in agar plates was used as a marker of herbicide sensitivity in the presence or absence of NBD-Cl and malathion (P450s inhibitor). The results provide evidence that P450s are involved in NTSR in the population of A. palmeri of this study, as previously reported, and they suggest, for the first time in a dicot weed, the involvement of GSTs in sulfonylurea metabolism.

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非靶位抗磺脲类苋的谷胱甘肽代谢和游离氨基酸谱

尽管大多数苋属植物对ALS抑制剂的抗性是由于酶的改变（靶点抗性机制），但也有一些抗性是由于除草剂代谢（非靶点（NTS）机制）。本研究研究了a . palmeri (R) NTSR致敏感群体(S)和磺脲抗性群体(S)的生理特性。这两个群体都在水培中生长，并使用ALS抑制剂nico磺隆，单独或与NBD-Cl （GST抑制剂）联合处理。7 d后，测定叶片中游离氨基酸谱、谷胱甘肽（GSH）含量和GST活性及表达。只有S个群体的叶片显示出先前描述的磺脲酶作用后的典型氨基酸分布；R群体没有发生变化，表明通过除草剂代谢维持ALS活性，从而防止了这种生理破坏。在S种群中，除草剂诱导的氧化应激引发GST活性增加和谷胱甘肽相关化合物（GSH及其前体）的积累。R群体的GST活性高于S群体，GST活性是由除草剂诱导的。对4个Phy和4个Tau gst进行了评估，但没有一个与活性增加明显相关。琼脂板上的根长作为除草剂敏感性的标志，在存在或不存在ndd - cl和马拉硫磷（p450抑制剂）的情况下。研究结果证实p450参与了本研究中棕榈属植物NTSR的表达，并首次在双科植物中发现gst参与了磺酰脲代谢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.