Plant Science最新文献_第5页

Comprehensive evaluation of the breeding value of OsPUP7 in rice OsPUP7在水稻育种价值的综合评价

IF 4.1 2区生物学

Plant Science Pub Date : 2025-09-24 DOI: 10.1016/j.plantsci.2025.112782

Chun Ye, Yile Sheng, Xiaohua Zhang, Jingwen Zhang, Hongzheng Sun, Chunbo Miao, Junzhou Li, Yanxiu Du

{"title":"Comprehensive evaluation of the breeding value of OsPUP7 in rice","authors":"Chun Ye, Yile Sheng, Xiaohua Zhang, Jingwen Zhang, Hongzheng Sun, Chunbo Miao, Junzhou Li, Yanxiu Du","doi":"10.1016/j.plantsci.2025.112782","DOIUrl":"10.1016/j.plantsci.2025.112782","url":null,"abstract":"<div><div>Gene editing has emerged as a powerful tool in crop breeding, particularly for rice, enabling the creation of germplasm and trait improvement. A thorough understanding of gene functions is essential for precise and efficient crop enhancement. <em>OsPUP7</em> encodes a purine permease involved in cytokinin transport. However, the effects of <em>OsPUP7</em> deficiency on rice growth and development, as well as its potential application value in rice breeding, remain unclear<em>.</em> This study analyzed the expression patterns of <em>OsPUP7</em> in rice, constructed <em>OsPUP7</em> knockout lines, and conducted three consecutive years of field trials to systematically evaluate the effects of <em>OsPUP7</em> knockout on rice agronomic traits, yield traits, quality, resistance, and seed germination. The results showed that <em>OsPUP7</em> is primarily expressed in panicles and grains. <em>OsPUP7</em> knockout had no significant effect on heading date, plant height, number of panicles per plant, panicle length, primary branches, or single-plant yield, but significantly reduced the number of secondary branches and grains per panicle. At the same time, <em>OsPUP7</em> knockout significantly increased grain length and the length-to-width ratio, reduced grain thickness, but had no significant effect on grain width and thousand-grain weight. In addition, the knockout of <em>OsPUP7</em> generally does not affect milling quality, nutritional properties, or cooking quality. <em>OsPUP7</em> knockout also had no significant effect on blast resistance, salt tolerance and seed germination. Based on the comprehensive evaluation above, we conclude that <em>OsPUP7</em> is a potential and efficient target gene for improving rice grain shape using gene editing technology.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112782"},"PeriodicalIF":4.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen sulfide as a key mediator in melatonin-induced enhancement of cold tolerance in tomato (Solanum lycopersicum L.) seedlings 硫化氢在褪黑激素诱导的番茄抗寒性增强中的作用幼苗。

IF 4.1 2区生物学

Plant Science Pub Date : 2025-09-22 DOI: 10.1016/j.plantsci.2025.112784

Cheng Ma , Zi-Qi Pei , Qiao Zhu, Cai-Hong Chai, Tong-Guo, Xin-Xin Mou, Xu-Wang, Juan Wang, Teng-Guo Zhang, Sheng Zheng

{"title":"Hydrogen sulfide as a key mediator in melatonin-induced enhancement of cold tolerance in tomato (Solanum lycopersicum L.) seedlings","authors":"Cheng Ma , Zi-Qi Pei , Qiao Zhu, Cai-Hong Chai, Tong-Guo, Xin-Xin Mou, Xu-Wang, Juan Wang, Teng-Guo Zhang, Sheng Zheng","doi":"10.1016/j.plantsci.2025.112784","DOIUrl":"10.1016/j.plantsci.2025.112784","url":null,"abstract":"<div><div>Melatonin (MT) is a key bioactive molecule that enhances plant tolerance to cold stress; however, the underlying mechanisms remain unclear. This study demonstrates that foliar application of 100 μM MT improves cold tolerance in tomato seedlings by activating antioxidant enzyme systems and cold-responsive pathways. Notably, endogenous hydrogen sulfide (H<sub>2</sub>S) levels are also elevated, suggesting a potential role in MT-mediated responses. Moreover, treatment with sodium hydrosulfide (NaHS, an H<sub>2</sub>S donor) further enhances MT-induced cold tolerance by increasing antioxidant capacity, promoting the accumulation of photosynthetic pigments and secondary metabolites, and reducing cold-induced oxidative damage. In contrast, inhibition of H<sub>2</sub>S signaling using HT (an H<sub>2</sub>S scavenger) or PAG (an inhibitor of H<sub>2</sub>S biosynthesis) significantly attenuates the protective effects of MT. Additionally, tomato lines overexpressing the H<sub>2</sub>S biosynthetic gene <em>SlDCD1</em> exhibit a more pronounced response to MT-induced cold tolerance than wild-type plants. Conversely, CRISPR/Cas9-mediated knockout of <em>SlDCD1</em> significantly compromises the protective effects of MT under cold stress. Collectively, the results suggest that H<sub>2</sub>S signaling plays a central role in MT-mediated cold stress tolerance in tomato seedlings. This work provides new insights into how MT contributes to plant adaptation under cold stress.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112784"},"PeriodicalIF":4.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glutathione metabolism and free amino acid profile in Amaranthus palmeri with non-target site-based resistance to sulfonylureas 非靶位抗磺脲类苋的谷胱甘肽代谢和游离氨基酸谱

IF 4.1 2区生物学

Plant Science Pub Date : 2025-09-20 DOI: 10.1016/j.plantsci.2025.112781

Mikel V. Eceiza , Miriam Gil-Monreal , Clara Jimenez-Martinez , Laura Estepa , Eneko Trebol-Aizpurua , Mithila Jugulam , Mercedes Royuela , Ana Zabalza

{"title":"Glutathione metabolism and free amino acid profile in Amaranthus palmeri with non-target site-based resistance to sulfonylureas","authors":"Mikel V. Eceiza , Miriam Gil-Monreal , Clara Jimenez-Martinez , Laura Estepa , Eneko Trebol-Aizpurua , Mithila Jugulam , Mercedes Royuela , Ana Zabalza","doi":"10.1016/j.plantsci.2025.112781","DOIUrl":"10.1016/j.plantsci.2025.112781","url":null,"abstract":"<div><div>Although the majority of <em>Amaranthus palmeri</em> populations resistant to ALS inhibitors are due to alterations in the enzyme (target-site resistance mechanism), there are some cases of resistance due to herbicide metabolization (which is a non-target site (NTS) mechanism). In this study, we investigated the physiology of a sensitive (S) population and a sulfonylurea-resistant population due to NTSR of <em>A. palmeri</em> (R). Both populations were grown hydroponically and treated with the ALS inhibitor nicosulfuron, alone or combined with NBD-Cl (GST inhibitor). Seven days later, free amino acid profile, glutathione (GSH) content and GST activity and expression were measured in the leaves. Only S population leaves showed the typical amino acid profile previously described after sulfonylureas; R population showed no change, suggesting that such physiological disruption is prevented as ALS activity is maintained through herbicide metabolism. In the S population, the herbicide-induced oxidative stress triggered an increase in GST activity and accumulation of glutathione-related compounds (GSH and its precursors). R population showed a higher basal GST activity than S population and GST activity was induced by the herbicide. Three Phy and three Tau GSTs were evaluated, but none were clearly linked to increased activity. Root length in agar plates was used as a marker of herbicide sensitivity in the presence or absence of NBD-Cl and malathion (P450s inhibitor). The results provide evidence that P450s are involved in NTSR in the population of <em>A. palmeri</em> of this study, as previously reported, and they suggest, for the first time in a dicot weed, the involvement of GSTs in sulfonylurea metabolism.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112781"},"PeriodicalIF":4.1,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acrolein metabolism and its relationship to the occurrence of water-soaked brown flesh in Japanese pear fruits 日本梨果实丙烯醛代谢及其与水浸褐肉发生的关系。

IF 4.1 2区生物学

Plant Science Pub Date : 2025-09-19 DOI: 10.1016/j.plantsci.2025.112778

Nobuyuki Fukuoka , Tatsuro Hamada , Toshihiro Yamamura , Mikiko Sasazuka

{"title":"Acrolein metabolism and its relationship to the occurrence of water-soaked brown flesh in Japanese pear fruits","authors":"Nobuyuki Fukuoka , Tatsuro Hamada , Toshihiro Yamamura , Mikiko Sasazuka","doi":"10.1016/j.plantsci.2025.112778","DOIUrl":"10.1016/j.plantsci.2025.112778","url":null,"abstract":"<div><div>Water-soaked brown flesh (WSBF) of pear (<em>Pyrus pyrifolia</em> var. <em>culta</em>) is a physiological disorder in which the flesh of the fruit turns brown in the late stage of maturation. Since it is not possible to determine the presence or absence of the disorder from the external appearance, it is difficult to remove the affected fruit during harvesting, sorting, and preparation work, resulting in significant losses not only for producers but also for consumers. In this study, we compared the metabolite contents and gene expression levels involved in acrolein (ACR) production in mature pear fruit of WSBF-susceptible and -resistant cultivars. WSBF occurred in the ripe fruit of the susceptible cultivar, but no WSBF was observed in the resistant cultivar, even in overripe fruit. ACR was significantly accumulated in the flesh tissue of ripe susceptible cultivar fruit, but little ACR accumulation was observed in the resistant cultivar, even in overripe fruit. The analysis of metabolite contents and gene expression levels showed that the gene expression levels of the polyamine metabolism and ethylene synthesis pathway were higher in the susceptible cultivar than in the resistant cultivar, indicating that these metabolic pathways were activated. Furthermore, the expression levels of genes involved in the decomposition of polyamines and lipids were also maintained at a higher level in the former compared to the latter. The results revealed that both the activation of the ethylene synthesis pathway and the subsequent active synthesis and degradation of polyamines leading to the production of ACR, and the increased activity of lipid-degrading enzymes due to activation of the ethylene synthesis pathway may be closely related to the occurrence of WSBF disorder in pear fruit.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112778"},"PeriodicalIF":4.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the mechanistic insight and relevance of root hair-driven rhizobia for developing climate-smart crops 了解根毛驱动根瘤菌的机理和相关性，以开发气候智能型作物。

IF 4.1 2区生物学

Plant Science Pub Date : 2025-09-18 DOI: 10.1016/j.plantsci.2025.112779

Sudhir Kumar Upadhyay , Prasann Kumar , Devendra Jain

{"title":"Understanding the mechanistic insight and relevance of root hair-driven rhizobia for developing climate-smart crops","authors":"Sudhir Kumar Upadhyay , Prasann Kumar , Devendra Jain","doi":"10.1016/j.plantsci.2025.112779","DOIUrl":"10.1016/j.plantsci.2025.112779","url":null,"abstract":"<div><div>Symbiosis between legumes and rhizobia is a basic biological process behind sustainable agriculture. Still, in abiotic circumstances, such as drought, salt, and extreme temperatures, its efficiency is significantly reduced. This review highlights the molecular and physiological mechanisms that regulate root hair-rhizobia interactions, as root hairs serve as essential interfaces for microbial recognition, signal transduction, and infection thread growth. Root hair development in effective rhizobia colonization is influenced by auxin, ethylene, and environmental factors. Reacting to host flavonoids, which are detected by LysM receptor kinases (NFR1/NFR5), rhizobia produce <em>nod</em> factors causing calcium oscillations and corresponding transcriptional reprogramming of CCaMK, NSP1/2, and NIN. The changes in the cytoskeleton, the signaling of reactive oxygen species (ROS), and the remodeling of the cell wall all work together to change the shape of root hairs and make it easier for infection pockets to form. Rhizobia can keep symbiosis going even when abiotic stress happens by using adaptive mechanisms such as making exopolysaccharides, storing osmolytes, boosting antioxidant activity, and changing phytohormones signal. Combining multi-omics technologies, precision breeding, and microbial engineering will significantly enhance our understanding and improve root hair-mediated long-term symbiotic performance. This strategy promotes sustainable growth by reducing fertilizer usage, improving soil health, and ensuring food security in the face of changing climatic conditions.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112779"},"PeriodicalIF":4.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sugar-gibberellin crosstalk mediates leaf elongation in rice (Oryza sativa L.) 糖-赤霉素串声介导水稻叶片伸长。

IF 4.1 2区生物学

Plant Science Pub Date : 2025-09-18 DOI: 10.1016/j.plantsci.2025.112737

Jiaqi Bai , Bingjie Liu , Weiyan Zhang , Yan Zhu , Ganghua Li , Zhenghui Liu , Yanfeng Ding , Lin Chen

{"title":"Sugar-gibberellin crosstalk mediates leaf elongation in rice (Oryza sativa L.)","authors":"Jiaqi Bai , Bingjie Liu , Weiyan Zhang , Yan Zhu , Ganghua Li , Zhenghui Liu , Yanfeng Ding , Lin Chen","doi":"10.1016/j.plantsci.2025.112737","DOIUrl":"10.1016/j.plantsci.2025.112737","url":null,"abstract":"<div><div>Leaf morphology and function influence the production and yield of photosynthetic carbohydrates, while leaf development requires an ample supply of energy and carbohydrates, regulated by sucrose transport proteins (OsSUTs). Loss of function mutation in OsSUTs lead to shortened leaves in rice; however, the underlying regulatory mechanism remains unclear. In this study, we investigated the growing leaves of <em>ossut</em> mutants, focusing on agronomic traits, leaf morphology and physiology, to determine the relationship between OsSUTs and leaf elongation. Results showed that the cell length in <em>ossut</em> mutants was significantly shortened, especially in the <em>ossut3;5</em> lines. Chlorophyll content, net photosynthetic rate, sucrose exudation rate, and sucrose phosphate synthase activity in source leaves were also significantly decreased in <em>ossut3;5</em>. These findings suggest that inhibited sucrose transport in <em>ossut</em> mutants caused overaccumulation of sucrose and starch in source leaves, consequently blocking sugar export to the growing sink leaves. Additionally, mutations in OsSUTs affected leaf gibberellin (GA) content. Transcriptome analysis revealed that changes in the expression of genes related to GA biosynthesis, degradation, and signaling were the main causes of decreased GA levels in leaves. Furthermore, exogenous application of GA to rice leaves significantly increased both leaf and leaf cell lengths in all lines, whereas treatment with the GA synthesis inhibitor paclobutrazol (PAC) had the opposite effects. These results imply that the crosstalk between sucrose and GA potentially regulates rice leaf elongation.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112737"},"PeriodicalIF":4.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rooting for survival: bZIP89 tunes cell-specific root plasticity and drought adaptation in maize 根系生存：bZIP89调控玉米细胞特异性根系可塑性和干旱适应。

IF 4.1 2区生物学

Plant Science Pub Date : 2025-09-17 DOI: 10.1016/j.plantsci.2025.112774

Hameed Gul , Shareef Gul , Ali Shahzad

引用次数: 0

The role of root phene interactions in zinc and copper uptake efficiency in maize under NPK fertilization 氮磷钾施肥下根烯互作对玉米锌、铜吸收效率的影响

IF 4.1 2区生物学

Plant Science Pub Date : 2025-09-16 DOI: 10.1016/j.plantsci.2025.112777

Prakriti Rajput , Shubham Sharma , Haroon Rashid Hakla , Urfan Mohammad , Bhubneshwari Khajuria , Gurdev Chand , Dhiraj Vyas , Sikander Pal

{"title":"The role of root phene interactions in zinc and copper uptake efficiency in maize under NPK fertilization","authors":"Prakriti Rajput , Shubham Sharma , Haroon Rashid Hakla , Urfan Mohammad , Bhubneshwari Khajuria , Gurdev Chand , Dhiraj Vyas , Sikander Pal","doi":"10.1016/j.plantsci.2025.112777","DOIUrl":"10.1016/j.plantsci.2025.112777","url":null,"abstract":"<div><div>The role of root phene (s) and root phene modules in micronutrient uptake under a regular supply of NPK are least understood in <em>Zea mays</em> L. (maize). Exploration of collective and individual root behavior under different treatments of excessive macronutrients (N, P, K) or NPK and their impact on the uptake of micronutrients (Zn and Cu) was compared. The positive impact of N fertilizer on Zn and Cu uptake efficiency was associated with Lateral root weight (LRW) x Root cortical aerenchyma (RCA) root phene module (M1, <em>R</em><sup>2</sup> 0.079 and 0.906). The negative impact of P on Zn uptake efficiency was attributed to the M2 module (LRW x RCA x Nodal root number (NRN)) and the M1 module (<em>R</em><sup>2</sup> 0.209) for Cu uptake. Similarly, the negative impact of K fertilizer on Zn and Cu uptake efficiencies was associated with M2 modules respectively. Furthermore, root phene module behavior for positive or negative impact on Zn and Cu uptake was correlated with up-regulation or down-regulation of Zn and Cu transporters in all the root types. The maximum reduction in root exploration cost was observed for N alone followed by NPK and K. The findings are important in understanding the root exploration cost and root phene module management to control the impact of N, P, K, and NPK fertilizers on Zn and Cu uptake efficiencies in maize.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112777"},"PeriodicalIF":4.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intrinsic responses to nitrogen deficiency of Antarctic ecotypes of Colobanthus quitensis (Kunth) Bartl. and Deschampsia antarctica Desv. 南极五花子生态型对缺氮的内在响应和Deschampsia antarctica Desv。

IF 4.1 2区生物学

Plant Science Pub Date : 2025-09-16 DOI: 10.1016/j.plantsci.2025.112776

Nicolás Peña-Heyboer, Marisol Pizarro , Gustavo E. Zúñiga, Rodrigo A. Contreras

{"title":"Intrinsic responses to nitrogen deficiency of Antarctic ecotypes of Colobanthus quitensis (Kunth) Bartl. and Deschampsia antarctica Desv.","authors":"Nicolás Peña-Heyboer, Marisol Pizarro , Gustavo E. Zúñiga, Rodrigo A. Contreras","doi":"10.1016/j.plantsci.2025.112776","DOIUrl":"10.1016/j.plantsci.2025.112776","url":null,"abstract":"<div><div>Antarctic vascular plants <em>Colobanthus quitensis</em> and <em>Deschampsia antarctica</em> provide unique models for studying intrinsic adaptive responses to nutrient limitation, yet their responses to nitrogen deficiency remain poorly understood. Here, we investigated their ecophysiological adjustments under controlled axenic hydroponic conditions. After 15 days of nitrogen deprivation, both species exhibited pronounced root elongation, reflecting an adaptive shift to enhance nutrient acquisition. Nitrogen stress elevated reactive oxygen species (ROS) but did not increase malondialdehyde (MDA), indicating effective activation of antioxidant defenses. Enzyme assays revealed tissue-specific and enzyme-specific regulation: nitrate reductase (NR), nitrite reductase (NiR), and glutamate synthases (Fd-GOGAT, NADH-GOGAT) were generally downregulated, while glutamate dehydrogenase isoforms (NADPH-GDH, NADH-GDH) increased, particularly in older tissues, suggesting active nitrogen remobilization. Reduced phenylalanine ammonia-lyase (PAL) activity and total phenolic content highlighted a trade-off, diverting nitrogen from secondary metabolism to sustain primary functions. Altogether, these findings provide a comprehensive view of intrinsic strategies for nitrogen stress tolerance in Antarctic plants and offer translational insights for breeding crops with improved nitrogen use efficiency and resilience to climate-induced nutrient limitations.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112776"},"PeriodicalIF":4.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic and transcriptomic features of stress memory during salt-stress priming in arabidopsis 拟南芥盐胁迫启动过程中胁迫记忆的表观遗传学和转录组学特征。

IF 4.1 2区生物学

Plant Science Pub Date : 2025-09-15 DOI: 10.1016/j.plantsci.2025.112775

Burcu Arıkan , Özgür Çakır , Neslihan Turgat Kara

{"title":"Epigenetic and transcriptomic features of stress memory during salt-stress priming in arabidopsis","authors":"Burcu Arıkan , Özgür Çakır , Neslihan Turgat Kara","doi":"10.1016/j.plantsci.2025.112775","DOIUrl":"10.1016/j.plantsci.2025.112775","url":null,"abstract":"<div><div>Plants can develop a ‘stress memory’ that enables them to respond more effectively upon re-exposure to stress. It has been reported that priming plays a role in the formation of stress memory. In this study, Arabidopsis plants were primed with 50 mM NaCl for 24 h. Following a recovery period, they were subjected to subsequent salt stress, and it was observed that the primed plants were significantly less affected compared to the non-primed group. Furthermore, genes associated with epigenetic memory influencing stress response were investigated through RNA sequencing and ChIP analyses following the priming and salt stress applications. These analyses identified alpha-linolenic acid metabolism, diterpenoid biogenesis, and plant hormone signal transduction pathways as the most significantly altered pathways related with stress memory. Additionally, ChIP-qPCR analyses were performed on key genes within these pathways. RNA-seq analysis and changes in H3K27me3 and H3K4me histone marks were correlated, specifically in <em>OPR3</em>, <em>AOC1</em>, and <em>LOX3</em> genes that enabled us to obtain strong evidence regarding their effects on plant stress memory. The findings are expected to guide future studies in manipulating the identified candidate genes involved in epigenetic stress memory, thereby contributing to a deeper understanding of the molecular mechanisms underlying these processes.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112775"},"PeriodicalIF":4.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0