Plant Science最新文献

{"title":"The MdWRKY17 positively regulates nitrate uptake by promoting MdNRT2.5 expression under long-term low N stress in apple","authors":"Zehui Hu,&nbsp;Dongqian Shan,&nbsp;Chanyu Wang,&nbsp;Yixue Bai,&nbsp;Tianci Yan,&nbsp;Tong Zhang,&nbsp;Handong Song,&nbsp;Ruoxue Li,&nbsp;Yixuan Zhao,&nbsp;Qian Deng,&nbsp;Changjian Dai,&nbsp;Peiyun Xiao,&nbsp;Silong Dong,&nbsp;Jin Kong","doi":"10.1016/j.plantsci.2025.112402","DOIUrl":"10.1016/j.plantsci.2025.112402","url":null,"abstract":"<div><div>Nitrogen (N) supply is critical for apple yield and quality. Improving nitrogen use efficiency (NUE) could reduce fertilizer application for maintaining apple yield at the cost of environmental pollution in infertile soil. The molecular mechanisms underlying nitrate (NO₃^-) uptake are foundational for breeding high NUE cultivars. The two-month low N treatment mimicking infertile soil dramatically induced the accumulation of transcription factor MdWRKY17 in apple. Overexpression of <em>MdWRKY17</em> conferred enhanced long-term low nitrogen tolerance in transgenic apple plants and calli, while RNA interference of <em>MdWRKY17</em> reduced this tolerance. <em>MdNRT2.5</em> encoding a high-affinity nitrate transporter was identified by chromatin immunoprecipitation sequencing (ChIP-seq) as the direct target of MdWRKY17. This is confirmed by <em>in vitro</em> EMSA and <em>in vivo</em> ChIP-qPCR assay. Notably, overexpression of <em>MdNRT2.5</em> increased NO₃^- uptake under long-term N-deficiency conditions. RNA interference of <em>MdNRT2.5</em> in roots decreased NO₃^- uptake efficiency of <em>MdWRKY17</em>-OE transgenic apple plants, indicating that MdWRKY17 improves NO₃^- uptake mainly by activating <em>MdNRT2.5</em> expression. Our study identified an important <em>MdWRKY17-MdNRT2.5</em> module in response to long-term low N stress, which will contribute to the molecular breeding of high NUE apple cultivars.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112402"},"PeriodicalIF":4.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volatile signaling in weed plant Ageratina adenophora: Understanding the key emissions influencing Procecidochares utilis attraction to gall formation","authors":"Nipapan Kanjana ,&nbsp;Yuyan Li ,&nbsp;Muhammad Afaq Ahmed ,&nbsp;Lin Ma ,&nbsp;Lisheng Zhang","doi":"10.1016/j.plantsci.2025.112404","DOIUrl":"10.1016/j.plantsci.2025.112404","url":null,"abstract":"<div><div>The stem gall fly (<em>Procecidochares utilis</em>) significantly impacts host–plant biology by inhabiting specific parts of stem tissue, ensuring its own survival. Despite this, comprehensive identification of the primary bioactive compounds within host plants that are involved in gall formation remains elusive. This study aims to elucidate the crucial volatile compounds utilized by gall flies to alter host–plant defenses, either through direct or indirect manipulation via the release of an enticing volatile compound attractive to the fly. Employing Y-tube olfactometer assays, we examined the response of <em>Procecidochares utilis</em> to host plants from three Asteraceae weed species—<em>Ageratina adenophora</em>, <em>Ageratum conyzoides</em>, and <em>Praxelis clematidea</em>. Volatile compounds were extracted using headspace solid-phase microextraction (HS-SPME) and SPME-FIBER. Subsequently, gas chromatography-electroantennography and electroantennography were employed to analyze the antennal responses to individual odorants. The analysis revealed that the primary bioactive compound varied among the three weed species. Out of a total of 805 known volatiles, 65 main active compounds were exclusive to <em>Ageratina adenophora</em> (host plant). Remarkably, only 8 bioactive compounds were identified to elicit an antennal response from <em>Procecidochares utilis</em>. Notably, caryophyllene, β-bisabolene, and 4-thujen-2-α-yl acetate exhibited the remarkable ability to elicit an attraction response from both sexes of <em>Procecidochares utilis</em>. Among these, β-bisabolene emerged as the key compound, eliciting the most significant response from the gall fly antenna. Our findings offer novel insights into the specific attraction of the stem gall fly to <em>Ageratina adenophora</em>, utilizing key odorants as unique cues for initiating gall formation on its host plant. This discovery highlights how these cues enable the gall fly to exert direct or indirect control over its host. Additionally, these findings underscore the potential of this approach in the development of sustainable pest management strategies in the context of field trials.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112404"},"PeriodicalIF":4.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 14–3–3 gene AaGRF1 positively regulates cold tolerance in kiwifruit","authors":"Zhang Zhenzhen ,&nbsp;Li Sumei ,&nbsp;Sun Shihang ,&nbsp;Li Hongli ,&nbsp;Zhang Qina ,&nbsp;Li Yihang ,&nbsp;Li Yukuo ,&nbsp;Liu Mingyu ,&nbsp;Li Congcong ,&nbsp;Sun Leiming ,&nbsp;Lin Miaomiao ,&nbsp;Qi Xiujuan","doi":"10.1016/j.plantsci.2025.112403","DOIUrl":"10.1016/j.plantsci.2025.112403","url":null,"abstract":"<div><div>Low temperatures severely threaten the growth and development of kiwifruit. Research has demonstrated that proteins belonging to the 14–3–3 family play a pivotal regulatory function in the ability of plants to resist stress. However, this specific roles of the genes in kiwifruit cold tolerance remain unclear. It had been identified that β-amylase gene, <em>AaBAM3.1</em>, exhibits a positive regulatory effect on kiwifruit's tolerance to low temperature. In our research, we obtained the <em>Actinidia arguta</em> 14–3–3 gene general regulatory factor 1 (<em>AaGRF1</em>) from yeast one-hybrid (Y1H) screening library of the <em>AaBAM3.1</em> promoter; the expression level of <em>AaGRF1</em> was enhanced by low-temperature stress. Subcellular localization, Y1H and dual-LUC assay indicated that the AaGRF1 protein resides within the nucleus and possesses the ability to interact with the <em>AaBAM3.1</em> promoter. Moreover, we also studied the role of <em>AaGRF1</em> gene in cold resistance of kiwifruit. When <em>AaGRF1</em> was overexpressed in kiwifruit, the transgenic plants exhibited enhanced cold tolerance. The level of antioxidants and soluble sugars in these plants were elevated compared to wild-type (WT) lines. RNA-seq of the transgenic and WT lines revealed that <em>AaGRF1</em> might interact with genes in the ‘ascorbate-glutathione’ and ‘starch and sucrose’ pathways, thereby enhancing the cold resistance of kiwifruit. In summary, we hypothesize that the 14–3–3 gene <em>AaGRF1</em> may positively modulate the cold resistance in kiwifruit by accumulating more antioxidants and soluble sugars.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112403"},"PeriodicalIF":4.2,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PLATZ transcription factors and their emerging roles in plant responses to environmental stresses","authors":"Hongxia Zhang ,&nbsp;Lu Liu ,&nbsp;Zhenzhen Li ,&nbsp;Shuo Wang ,&nbsp;Li Huang ,&nbsp;Sue Lin","doi":"10.1016/j.plantsci.2025.112400","DOIUrl":"10.1016/j.plantsci.2025.112400","url":null,"abstract":"<div><div>Plant A/T-rich sequence- and zinc-binding (PLATZ) family proteins represent a novel class of plant-specific transcription factors that bind to A/T-rich sequences. Advances in high-throughput sequencing and bioinformatics analyses have facilitated the identification of numerous PLATZ proteins across various plant species. Over the last decade, accumulating evidence from omics analyses, genetics studies, and gain- and loss-of function investigations has indicated that PLATZ proteins play crucial roles in the complex regulatory networks governing plant development and adaptation to environmental stress. Recently, an excellent review has been published highlighting the roles of PLATZ proteins in controlling plant developmental processes. However, a comprehensive review specifically addressing the molecular mechanisms by which these proteins drive their functions in plant responses to environmental cues is currently lacking. In this review, we summarize the characteristics and identification of PLATZ proteins, emphasizing their significance in stress responses. We also highlight the crosstalk between PLATZ proteins and phytohormones. Furthermore, we discuss the downstream target genes, interacting partners, and upstream regulatory mechanisms associated with PLATZ proteins, providing a thorough understanding of their multifaceted roles in plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112400"},"PeriodicalIF":4.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpressing OsNF-YB12 elevated the content of jasmonic acid and impaired drought tolerance in rice","authors":"Zhijuan Bian ,&nbsp;Meiyao Chen ,&nbsp;Lei Wang ,&nbsp;Xiaosong Ma ,&nbsp;Qing Yu ,&nbsp;Zixin Jia ,&nbsp;Jinghong Wu ,&nbsp;Haibin Wei ,&nbsp;Hongyan Liu ,&nbsp;Yi Liu ,&nbsp;Lijun Luo ,&nbsp;Shunwu Yu","doi":"10.1016/j.plantsci.2025.112397","DOIUrl":"10.1016/j.plantsci.2025.112397","url":null,"abstract":"<div><div>Nuclear factor Y (NF-Y) is an evolutionarily conserved heterotrimeric transcription factor in eukaryotes. In a previous study, <em>OsNF-YB12</em> was confirmed to be associated with drought tolerance using the Ecotilling method. In this study, real-time quantitative RT-PCR revealed that <em>OsNF-YB12</em> was induced by various abiotic stresses and phytohormones, with expression levels differing between leaves and roots. Rice overexpressing <em>OsNF-YB12</em> was more sensitive to salinity and PEG osmotic stresses at seed germination stage, as well as reduced drought tolerance at seedling stage. Notably, the accumulation of free proline and photosynthetic efficiency was significantly declined in <em>OsNF-YB12</em> transgenic plants following osmotic stimuli. Transcriptomic analysis of transgenic <em>OsNF-YB12</em> plants indicated that OsNF-YB12 could upregulate terpene metabolism related to defense responses and the expression levels of JAZ proteins under normal conditions, while downregulating osmotic stress-related regulatory genes under osmotic stress, in comparison to the wild type. Further analysis revealed that overexpressing <em>OsNF-YB12</em> promoted JA biosynthesis and inhibit seed germination. Haplotype analysis suggested that <em>OsNF-YB12</em> may have been selected during the differentiation of <em>indica</em> and <em>japonica</em> rice varieties. Therefore, this research provides a potential molecular target for exploring and harnessing the haplotype diversity of <em>OsNF-YB12</em> to enhance yield stability under drought stress during rice domestication and improvement.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112397"},"PeriodicalIF":4.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amino acid transporter OsATL13 coordinately regulates rice yield and quality by transporting phenylalanine and methionine","authors":"Lianxin Ding ,&nbsp;Weiting Huang ,&nbsp;Zhenghan Li,&nbsp;Zhongming Fang","doi":"10.1016/j.plantsci.2025.112398","DOIUrl":"10.1016/j.plantsci.2025.112398","url":null,"abstract":"<div><div>Amino acids are crucial nutrients for growth in crops. In this study, we found an amino acid transporter-like 13 (<em>OsATL13</em>), that coordinately determined rice yield and quality. <em>OsATL13</em> was primarily expressed in the root and panicle, its protein was localized on plasma membrane, and it principally transported phenylalanine and methionine. Overexpression (OE) of <em>OsATL13</em> increased the tiller number by 31.4 %, resulting in a 16.18 % increase in grain yield compared to Zhonghua 11 (ZH11). It also decreased amylose content and increased protein content in <em>OsATL13</em> OE lines compared to ZH11, whereas the <em>OsATL13</em> mutant exhibited opposite effects. RNA-seq analysis revealed that upregulation of <em>OsATL13</em> influenced the expression of genes associated with nitrogen and starch metabolism pathways. Notably, exogenous treatment with phenylalanine and methionine promoted axillary buds outgrowth, increased tiller number and rice yield, improved milled and head rice rates, and decreased chalky rice rate. Furthermore, rapid viscosity analysis supported the observation that phenylalanine and methionine treatments influenced rice eating and cooking quality. This research offers new perspectives on the synchronized enhancement of both rice yield and quality with amino acid transporter <em>OsATL13</em>.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"353 ","pages":"Article 112398"},"PeriodicalIF":4.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory networks of bZIPs in drought, salt and cold stress response and signaling","authors":"Yanli Yang ,&nbsp;Yi Xu ,&nbsp;Baozhen Feng ,&nbsp;Peiqian Li ,&nbsp;Chengqi Li ,&nbsp;Chen-Yu Zhu ,&nbsp;Shu-Ning Ren ,&nbsp;Hou-Ling Wang","doi":"10.1016/j.plantsci.2025.112399","DOIUrl":"10.1016/j.plantsci.2025.112399","url":null,"abstract":"<div><div>Abiotic stresses adversely impact plants survival and growth, which in turn affect plants especially crop yields worldwide. To cope with these stresses, plant responses depend on the activation of molecular networks cascades, including stress perception, signal transduction, and the expression of specific stress-related genes. Plant bZIP (basic leucine zipper) transcription factors are important regulators that respond to diverse abiotic stresses.By binding to specific <em>cis</em>-elements, bZIPs can control the transcription of target genes, giving plants stress resistance. This review describes the structural characteristics of bZIPs and summarizes recent progress in analyzing the molecular mechanisms regulating plant responses to salinity, drought, and cold in different plant species. The main goal is to deepen the understanding of bZIPs and explore their value in genetic improvement of plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112399"},"PeriodicalIF":4.2,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of cold-responsive cis-element in the OsPHD13 and OsPHD52 promoter and its upstream regulatory proteins in rice","authors":"Zimeng Wang ,&nbsp;Hao Li ,&nbsp;Juan Li ,&nbsp;Yachun Yang ,&nbsp;Zuntao Xu ,&nbsp;Jianbo Yang ,&nbsp;Pengcheng Wei ,&nbsp;Hui Ma","doi":"10.1016/j.plantsci.2025.112396","DOIUrl":"10.1016/j.plantsci.2025.112396","url":null,"abstract":"<div><div>Rice (<em>Oryza sativa</em> L.) is one of the most important grain crops in the world. Abiotic stress such as low temperature is an important factor affecting the yield and quality of rice. To explore the endogenous stress-resistant genes and apply them to the breeding of new stress-resistant varieties is an effective way to improve the stress tolerance and adaptability of rice. PHD-finger transcription factor is a kind of zinc-finger structural protein that exists widely in eukaryotes. Its function is mainly focused on gene transcription and regulation of chromatin state, but there are few reports about its involvement in stress response. In the present study, a total of 58 PHD-finger transcription factors were identified, and two genes <em>OsPHD13</em> and <em>OsPHD52</em> were significantly up-regulated under low temperature stress. After low temperature induction, <em>GUS</em> driven by <em>OsPHD13</em> and <em>OsPHD52</em> promoters had different expression activities in roots, stems and leaves of transgenic plants. Further functional analysis of the <em>pOsPHD13</em> and <em>pOsPHD52</em> showed that each of them had a cis-acting element of CRT/DRE in response to low temperature stress. Both in yeast one-hybrid assays and in in vitro gel-shift analysis, CBF protein could specifically bind to the CRT/DRE element in the promoter.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112396"},"PeriodicalIF":4.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The allelic mutation of NBS-LRR gene causes premature senescence in wheat","authors":"Lin Qiu ,&nbsp;Rongmin Fang ,&nbsp;Yong Jia ,&nbsp;Hongchun Xiong ,&nbsp;Yongdun Xie ,&nbsp;Linshu Zhao ,&nbsp;Jiayu GU ,&nbsp;Shirong Zhao ,&nbsp;Yuping Ding ,&nbsp;Chengdao LI ,&nbsp;Huijun Guo ,&nbsp;Luxiang Liu","doi":"10.1016/j.plantsci.2025.112395","DOIUrl":"10.1016/j.plantsci.2025.112395","url":null,"abstract":"<div><div>Premature senescence has a significant impact on the yield and quality of wheat crops. The process is controlled by multiple and intricate genetic pathways and regulatory elements, whereby the discovery of additional mutants provides important insights into the molecular basis of this important trait. Here, we developed a premature senescence wheat mutant <em>je0874</em>, its leaves started to show yellow before heading stage; with plant growth and development, the degree of yellowing worsened rapidly, and chlorophyll content in flag leaf was reduced by 93.8 % at 15 days after heading, all other leaves became dryness at the grain filling stage. In the mutant, the reactive oxygen species (ROS) and its metabolites increased up to 34.8–47.3 %, while activities of ROS scavenging enzymes were reduced by 62.7–96.7 %. Premature senescence resulted in a reduction of thousand grain weight by over 50 %. Genetic analysis showed the mutation of senescence was controlled by a single recessive gene, and target gene was finely mapped to a 338 kb region of the long arm of chromosome 2D. This region contained a total of 6 annotated genes, while only gene <em>TraesFLD2D01G513900</em> carried a SNP mutation. The gene contained an NBS-LRR domain, we named it <em>Taps1</em>. Allelic mutants of <em>Taps1</em> exhibited a lesion mimic phenotype, and the mutant allele resulted in cell death in tobacco, which represent a novel gene controlling wheat senescence. Two haplotypes were identified in 180 accessions, which did not lead to cell death. These results contribute to increase our understanding of the regulation of premature plant senescence.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112395"},"PeriodicalIF":4.2,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress-responsive plasma membrane H+-ATPases regulate deep rooting in rice","authors":"Di Wang ,&nbsp;Kai Xu ,&nbsp;Shoujun Chen ,&nbsp;Lei Wang ,&nbsp;Qiaojun Lou ,&nbsp;Changsen Zhong ,&nbsp;Yawen Wang ,&nbsp;Tiemei Li ,&nbsp;Huaxiang Cheng ,&nbsp;Lijun Luo ,&nbsp;Liang Chen","doi":"10.1016/j.plantsci.2025.112394","DOIUrl":"10.1016/j.plantsci.2025.112394","url":null,"abstract":"<div><div>Agricultural production is severely affected by environmental stresses such as drought, and deep rooting is an important factor enhancing crop drought avoidance. H⁺-ATPases provide a transmembrane proton gradient and are thought to play a crucial role in plant growth and abiotic stress responses. However, their expression under abiotic stress and function on deep rooting is poorly understood in rice. In this study, the conserved domains, potential phosphorylation sites, and three-dimensional structures of ten <em>Oryza sativa</em> PM H⁺-ATPases (OSAs) were analyzed. Quantitative PCR analysis revealed different expression patterns of these <em>OSA</em> genes under hormone treatment conditions (e.g., abscisic acid) and abiotic stress conditions (e.g., drought and salt stress). Subcellular localization analysis revealed that most OSA proteins were localized to the cell membrane. Phenotype determination of <em>OSA</em> mutants indicated that the ratio of deep rooting (RDR) of both <em>osa7</em> and <em>osa8</em> mutants was significantly reduced compared to that of wild-type rice plants. Additionally<em>, OSA</em> haplotypes in 268 rice accessions were analyzed, and the haplotypes associated with RDR were identified. The present results provide valuable information on crucial domains, expression patterns, and functional identification of OSA paralogs to reveal their role in rice responses to abiotic stress.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112394"},"PeriodicalIF":4.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}