Plant Science最新文献_第6页

SsWOX13, a novel sugarcane WOX transcription factor, confers disease resistance via HR-mediated programmed cell death in Nicotiana benthamiana: Insights from a genome-wide survey SsWOX13是一种新的甘蔗WOX转录因子，通过hr介导的benthamiana程序性细胞死亡赋予疾病抗性：来自全基因组调查的见解。

IF 4.1 2区生物学

Plant Science Pub Date : 2026-04-01 Epub Date: 2026-02-05 DOI: 10.1016/j.plantsci.2026.113024

Farhan Goher , Faiza Shafique Khan , Shuo-Hang Zhang , Shengren Sun , Wei Zhang , Yinjie Cheng , Qinnan Wang

{"title":"SsWOX13, a novel sugarcane WOX transcription factor, confers disease resistance via HR-mediated programmed cell death in Nicotiana benthamiana: Insights from a genome-wide survey","authors":"Farhan Goher , Faiza Shafique Khan , Shuo-Hang Zhang , Shengren Sun , Wei Zhang , Yinjie Cheng , Qinnan Wang","doi":"10.1016/j.plantsci.2026.113024","DOIUrl":"10.1016/j.plantsci.2026.113024","url":null,"abstract":"<div><div>WUSCHEL-related homeobox (WOX) transcription factors (TFs), which constitute a plant-specific homeodomain-containing family, play diverse roles in growth and development. However, their function in pathogen-induced stress responses remains largely unexplored. In this study, we identified 11 <em>SsWOX</em> family members in the sugarcane reference genome, which were classified into ancient, intermediate, and modern/WUSCHEL clades. Comparative analyses of motif composition, exon–intron organization, and <em>cis</em>-regulatory elements revealed conserved evolutionary relationships among <em>SsWOX</em> family genes. Expression profiling using RNA-seq and qRT-PCR during sugarcane–smut (<em>Sporisorium scitamineum</em>) interactions demonstrated distinct expression patterns of several <em>SsWOX</em> genes, with <em>SsWOX13</em> showing robust induction, indicating its potential role in smut resistance. Transient overexpression of <em>SsWOX13</em> in <em>Nicotiana benthamiana</em> led to hypersensitive response (HR)-associated programmed cell death, evidenced by elevated electrolyte leakage, increased reactive oxygen species accumulation, and upregulation of HR- and defense-related genes. Furthermore, transcriptional self-activation assays confirmed that <em>SsWOX13</em> possesses transcriptional activation activity, functioning as a TF in sugarcane. Collectively, these results expand our understanding of sugarcane <em>WOX</em> TFs and indicate that sugarcane <em>WOX13</em> positively regulates HR-mediated immunity.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113024"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146132910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

StMYB3 controls anthocyanin biosynthesis in potato via its dual regulatory role on StAN2 StMYB3通过对StAN2的双重调控作用控制马铃薯花青素的生物合成。

IF 4.1 2区生物学

Plant Science Pub Date : 2026-04-01 Epub Date: 2026-02-10 DOI: 10.1016/j.plantsci.2026.113052

Shuwen Wang , Yalan Ye , Xurui Yang , Fanbin Nie , Jianke Dong , Jianhua Zhou , Xijuan Zhao , Botao Song , Huiling Zhang

{"title":"StMYB3 controls anthocyanin biosynthesis in potato via its dual regulatory role on StAN2","authors":"Shuwen Wang , Yalan Ye , Xurui Yang , Fanbin Nie , Jianke Dong , Jianhua Zhou , Xijuan Zhao , Botao Song , Huiling Zhang","doi":"10.1016/j.plantsci.2026.113052","DOIUrl":"10.1016/j.plantsci.2026.113052","url":null,"abstract":"<div><div>Anthocyanins are a class of water-soluble flavonoid pigments that play crucial roles in plant physiology and human health. Their biosynthesis is regulated by the MYB-bHLH-WD40 transcriptional complex, in which MYB transcription factors serve as the key determinants. However, the function of repressor-type MYBs in tuber crops such as potato remains poorly understood. Here we show that the R2R3-MYB transcription factor <em>StMYB3</em> acts as a repressor of anthocyanin biosynthesis in potato. Transient-expression assays revealed that co-expression of StMYB3 with StAN2 (an R2R3 MYB activator) markedly reduced anthocyanin accumulation in leaves, and tuber skins of <em>StMYB3</em>-over-expressing lines exhibited a significant decrease in anthocyanin content. Mechanistic analyses demonstrate that both StMYB3 and StAN2 interact with StAN1 (an anthocyanin related bHLH). StMYB3 not only suppresses the StAN2-mediated transcriptional activation of anthocyanin biosynthesis genes (<em>StCHS</em>, <em>StF3H</em>, <em>StF3'5'H</em>, and <em>StGST</em>) but also directly represses the promoter activity of bHLH transcription factor <em>StAN1</em>. Moreover, <em>StMYB3</em> and <em>StAN2</em> form a mutually reinforcing positive-feedback loop at the transcriptional level. These results uncovers a StMYB3-StAN1-StAN2 regulatory module whose dynamics, driven by competition and bidirectional feedback, precisely control anthocyanin accumulation. This finding provides a reference for breeding potatoes with high anthocyanin content.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113052"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146181888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single nucleotide deletion in CsSCP4 disrupts brassinosteroid biosynthesis and confers a super compact phenotype in cucumber CsSCP4的单核苷酸缺失破坏了油菜素内酯的生物合成，并赋予黄瓜超紧凑的表型。

IF 4.1 2区生物学

Plant Science Pub Date : 2026-04-01 Epub Date: 2026-02-10 DOI: 10.1016/j.plantsci.2026.113050

Qiming Hu , Hamza Sohail , Yuanyuan Bian , BingKun Yang , Xiaodong Yang , Xuehao Chen , Xiaohua Qi , Xuewen Xu

{"title":"A single nucleotide deletion in CsSCP4 disrupts brassinosteroid biosynthesis and confers a super compact phenotype in cucumber","authors":"Qiming Hu , Hamza Sohail , Yuanyuan Bian , BingKun Yang , Xiaodong Yang , Xuehao Chen , Xiaohua Qi , Xuewen Xu","doi":"10.1016/j.plantsci.2026.113050","DOIUrl":"10.1016/j.plantsci.2026.113050","url":null,"abstract":"<div><div>Dwarf mutants in higher plants serve as ideal models for studying the mechanisms underlying stem elongation and plant development. Moreover, the compact stature of dwarf plants renders them advantageous for high-density planting and mechanized harvesting. In this study, we identified a naturally occurring super-compact cucumber mutant, designated scp159. Due to reduced cell elongation, scp159 exhibits an extremely compact plant morphology. Using bulk segregant analysis coupled with whole-genome sequencing and fine genetic mapping, we narrowed down the <em>scp-4</em> locus to a 67.6 kb region on chromosome 6. Through resequencing and molecular cloning, <em>CsSCP4</em> was identified as a candidate gene underlying the <em>scp-4</em> locus, which encodes 3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1, a key enzyme involved in brassinosteroid (BR) biosynthesis. Compared to the wild-type YZ205A, the scp159 mutant harbors a single-base deletion (adenine) within the fourth exon region of the <em>CsSCP4</em>. Measurements of endogenous hormone levels confirmed a significant reduction in BR levels in scp159 mutants. Exogenous application of brassinolide at a concentration of 0.2 mg/L effectively alleviated the super-compact phenotype. Silencing of the <em>CsSCP4</em> gene in cucumber plants resulted in reduced plant height. Comparative transcriptomic analysis further revealed significant downregulation of positive regulators of the BR signaling pathway, such as <em>BAK1</em> and <em>BSK</em>, and marked upregulation of the negative regulator <em>BKI1</em> in the scp159 mutant. Furthermore, differential expression was observed in genes associated with auxin and gibberellin biosynthesis and signaling pathways. Collectively, our findings demonstrate that the <em>CsSCP4</em> gene plays a crucial role in BR biosynthesis, thus significantly influencing cucumber plant growth and development.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113050"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146166335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of Kongyu 131 mutant library provides genetic resources for rice functional genomics and germplasm improvement 空育131突变文库的构建为水稻功能基因组学和种质改良提供遗传资源

IF 4.1 2区生物学

Plant Science Pub Date : 2026-04-01 Epub Date: 2026-01-31 DOI: 10.1016/j.plantsci.2026.113016

Tingting Xu , Qun Yang , Ran Li , Jianping Wang , Zhishan Luo , Daoyong Gong , Leihao Weng , Xiaoshan Huang , Debao Fu , Jing Li

{"title":"Construction of Kongyu 131 mutant library provides genetic resources for rice functional genomics and germplasm improvement","authors":"Tingting Xu , Qun Yang , Ran Li , Jianping Wang , Zhishan Luo , Daoyong Gong , Leihao Weng , Xiaoshan Huang , Debao Fu , Jing Li","doi":"10.1016/j.plantsci.2026.113016","DOIUrl":"10.1016/j.plantsci.2026.113016","url":null,"abstract":"<div><div>Kongyu 131 (<em>Oryza sativa</em> ssp. <em>japonica</em>) is an elite rice cultivar widely planted in Heilongjiang Province, China. Here, we subjected Kongyu 131 to ethyl methanesulfonate (EMS)-induced mutagenesis to establish a genome-wide mutant library. On this basis, we generated 8770 M2 lines, and systematically screened 2215 lines with 42 distinct phenotypic variations across all developmental stages. Then, we employed a three-dimensional (3D) pooling strategy to combine 6144 M3 DNA samples into 224 multiplexed pools. By using the TILLING-seq (Targeting Induced Local Lesions in Genomes sequencing) approach, we identified 84 mutants across five key genes (<em>GS3</em>, <em>Pi21</em>, <em>CSA</em>, <em>OsRR22</em>, and <em>OsaTRZ2</em>), and validated their allelic variations. Phenotypic screening generated 18 lines with superior plant architecture, including a novel <em>OsSPL14</em> allele identified via MutMap+ analysis. Whole-genome sequencing (WGS) of three M2 lines and TILLING-seq of mutants of the five key genes demonstrated a high mutation density (1 mutation per 121–288 kb). This 3D-pooled and genome-saturated EMS mutant library represents a robust resource for advancing functional genomic studies and precision breeding in rice.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113016"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146107015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An AT-hook motif nuclear protein AHL13 interacts with Poly(ADP-ribose) to regulate Arabidopsis immunity AT-hook基序核蛋白AHL13与Poly（adp -核糖）相互作用调节拟南芥免疫。

IF 4.1 2区生物学

Plant Science Pub Date : 2026-04-01 Epub Date: 2026-02-03 DOI: 10.1016/j.plantsci.2026.113021

Jia Liu , Zhuo Chen , Ling Sun , Tingyan Huang , Yu Chen , Xiaohui Li , Hu Liu , Xinyi Huang , Yan Peng , Baomin Feng

{"title":"An AT-hook motif nuclear protein AHL13 interacts with Poly(ADP-ribose) to regulate Arabidopsis immunity","authors":"Jia Liu , Zhuo Chen , Ling Sun , Tingyan Huang , Yu Chen , Xiaohui Li , Hu Liu , Xinyi Huang , Yan Peng , Baomin Feng","doi":"10.1016/j.plantsci.2026.113021","DOIUrl":"10.1016/j.plantsci.2026.113021","url":null,"abstract":"<div><div>Poly(ADP-ribosyl)ation or PARylation is required for immune transcription and defense against microbes in plants. However, the mechanisms underlying the PARylation-mediated transcriptional regulation are largely unknown. In this study, an AT-hook motif nuclear localized transcription factor, AHL13, was identified as an interactor of poly (ADP-ribose), the polymer products of PARylation. The knock-out and over-expression experiments suggest that AHL13 functions as a negative regulator of <em>Arabidopsis</em> immunity. RNA seq results showed that a group of defense-related genes were repressed by AHL13 upon bacterial infection. AHL13 could directly bind to the AT-rich sequences in the promoters of the target genes in the EMSA and MST assays. The PAR polymers directly interact with AHL13 with high affinity and significantly suppress its interaction with the AT-rich DNA, suggesting that PARylation might promote immune transcription through a repressor-repelling mechanism. In summary, this study revealed that the PAR-AHL13 interaction plays significant roles in immune gene expression in <em>Arabidopsis</em>.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113021"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146126229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Trichoderma-induced plant immunity: An RNA-epigenetic perspective 木霉诱导的植物免疫机制：rna -表观遗传学视角。

IF 4.1 2区生物学

Plant Science Pub Date : 2026-04-01 Epub Date: 2026-02-06 DOI: 10.1016/j.plantsci.2026.113030

Yifan Dong , Golam Jalal Ahammed

{"title":"Mechanisms of Trichoderma-induced plant immunity: An RNA-epigenetic perspective","authors":"Yifan Dong , Golam Jalal Ahammed","doi":"10.1016/j.plantsci.2026.113030","DOIUrl":"10.1016/j.plantsci.2026.113030","url":null,"abstract":"<div><div>Root-associated beneficial microbes can enhance plant resistance at the systemic level without triggering constitutive defense activation, yet how such durable and low-cost immune states are established remains poorly understood. Plant-<em>Trichoderma</em> spp. mutualism represents a well-characterized model of beneficial plant-microbe interactions in which induced resistance is maintained despite the absence of sustained transcriptional defense outputs. Accumulating evidence implicates small RNAs, including microRNAs (miRNAs), small interfering RNAs (siRNAs), and long non-coding RNAs (lncRNAs), as central regulators of immune priming during <em>Trichoderma</em>-plant interactions. Such RNA-mediated processes shape defense responsiveness, hormonal sensitivity, and chromatin-associated regulation. In parallel, epigenetic modifications have been linked to the persistence and reversibility of primed immune states. However, these regulatory layers are often examined separately, limiting mechanistic understanding of how immune states are both stabilized and flexibly reprogrammed. In this conceptual review, we synthesize recent advances to examine <em>Trichoderma</em>-induced systemic immunity through an integrated RNA-epigenetic perspective. By viewing RNA-mediated regulation and epigenetic modification as components of a functional continuum, we illustrate how immune priorities defined by small RNAs can be consolidated into chromatin states that preserve inducibility without imposing constitutive costs. This framework provides a coherent explanation for how <em>Trichoderma</em>-induced immunity is organized across molecular layers in plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113030"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146143157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

JsMYB170 modulates flavonols biosynthesis through facilitation of JsFLS1/5 expression in pellicle of Juglans sigillata Dode JsMYB170通过促进JsFLS1/5在核桃皮膜上的表达来调节黄酮醇的生物合成。

IF 4.1 2区生物学

Plant Science Pub Date : 2026-04-01 Epub Date: 2026-02-06 DOI: 10.1016/j.plantsci.2026.113032

Tiantian Fei , Chunxiang Li , Na Wang , Wen’e Zhang , Xuejun Pan

{"title":"JsMYB170 modulates flavonols biosynthesis through facilitation of JsFLS1/5 expression in pellicle of Juglans sigillata Dode","authors":"Tiantian Fei , Chunxiang Li , Na Wang , Wen’e Zhang , Xuejun Pan","doi":"10.1016/j.plantsci.2026.113032","DOIUrl":"10.1016/j.plantsci.2026.113032","url":null,"abstract":"<div><div><em>J. sigillata</em> produces abundant flavonols such as rutin and myricetin in pellicle, which provide various benefits to human health. However, the transcriptional regulation of flavonols biosynthesis in pellicle of <em>J. sigillata</em> remains unclear. Our study showed that the JsMYB170 is a transcriptional activator involved in the biosynthesis of flavonols in <em>J. sigillata</em>. In the pellicle of <em>J. sigillata</em>, the transient silencing of the <em>JsMYB170</em> gene can significantly reduce its expression level, as well as the expression of <em>JsFLS1/5</em> and the accumulation of flavonols such as rutin, myricetin, quercetin and kaempferol. In contrast, the transient over-expression of <em>JsMYB170</em> can significantly increase the content of flavonols in the pellicle. Similarly, qRT-PCR detection in mature fruits of <em>JsMYB170</em> over-expressing transgenic T<sub>3</sub> generation tomato showed that, the expression levels of <em>JsMYB170</em> and <em>SlFLS</em> also significantly increased. At the same time, HPLC analysis showed that the content of flavonols also significantly increased. Through DAP-seq, 25 downstream target genes of JsMYB170 were screened out. The dual luciferase assay conducted on tobacco leaves and the subsequent yeast one-hybrid assay both indicated that JsMYB170 can positively regulate the transcriptional expression of <em>JsFLS1/5</em> by binding to the AC element of the promoter. As a key enzyme gene for flavonol synthesis, the same transient expression of <em>JsFLS1/5</em> also showed that <em>JsFLS1/5</em> could positively regulate the accumulation of flavonols in pellicle of walnut. These research results provide new insights into the molecular regulatory pathways of functional substance flavonols accumulation on walnut pellicle.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113032"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146143093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyploidization disrupts drought response and epigenetic patterns in the desert wild potato species Solanum kurtzianum 沙漠野生马铃薯的多倍体化破坏了干旱响应和表观遗传模式。

IF 4.1 2区生物学

Plant Science Pub Date : 2026-04-01 Epub Date: 2026-02-02 DOI: 10.1016/j.plantsci.2026.113017

Damián N. Jerez , Carina V. González , Perla C. Kozub , Verónica N. Ibañez , Federico Berli , Ricardo W. Masuelli , Carlos F. Marfil

{"title":"Polyploidization disrupts drought response and epigenetic patterns in the desert wild potato species Solanum kurtzianum","authors":"Damián N. Jerez , Carina V. González , Perla C. Kozub , Verónica N. Ibañez , Federico Berli , Ricardo W. Masuelli , Carlos F. Marfil","doi":"10.1016/j.plantsci.2026.113017","DOIUrl":"10.1016/j.plantsci.2026.113017","url":null,"abstract":"<div><div>Polyploidy, the possession of more than two sets of chromosomes in a cell, is often linked to enhanced adaptability and stress resilience in plants, though it may introduce genomic instability and fitness costs. Here, we examined the interplay between ploidy level and epigenetic responses to drought stress in <em>Solanum kurtzianum</em>, a drought-tolerant wild relative of potato. We subjected diploid and newly oryzalin-induced autotetraploid lines to three different watering regimes and assessed their morphological, physiological, biochemical, and epigenetic responses. Diploids consistently outperformed autotetraploids in drought tolerance and tuber yield under moderate water stress, exhibiting lower stomatal conductance, yet maintaining comparable photochemical efficiency. Epigenetic analyses revealed significant interplay between the ploidy level and drought conditions on methylation patterns, with autotetraploids displaying higher methylation variability and greater genomic instability under severe drought. These findings are consistent with the predominance of diploid wild potatoes in arid regions and suggest that genomic instability caused by polyploidy may compromise drought resilience. The study emphasizes the adaptive potential of diploid wild potatoes in arid environments and the role of epigenetic mechanisms in stress responses. Our results have implications for potato breeding strategies, highlighting the potential of diploids for developing drought-resilient cultivars to cope with climate change challenges.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113017"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jasmonic acid affects epidermal cell fate determination via influencing cell cycle related gene expression in mangrove Avicennia marina 茉莉酸通过影响红树细胞周期相关基因的表达影响表皮细胞命运的决定。

IF 4.1 2区生物学

Plant Science Pub Date : 2026-04-01 Epub Date: 2026-02-03 DOI: 10.1016/j.plantsci.2026.113019

Xingyue Hong , Hanchen Tang , Hezi Huang , Mingyue Wei , Mengqi Wu , Zhaoyu Guo , Jiakun Liu , Lihan Zhuang , Ling Sun , Jicheng Wang , Hanxin Zheng , Hai-Lei Zheng

{"title":"Jasmonic acid affects epidermal cell fate determination via influencing cell cycle related gene expression in mangrove Avicennia marina","authors":"Xingyue Hong , Hanchen Tang , Hezi Huang , Mingyue Wei , Mengqi Wu , Zhaoyu Guo , Jiakun Liu , Lihan Zhuang , Ling Sun , Jicheng Wang , Hanxin Zheng , Hai-Lei Zheng","doi":"10.1016/j.plantsci.2026.113019","DOIUrl":"10.1016/j.plantsci.2026.113019","url":null,"abstract":"<div><div><em>Avicennia marina</em>, a pioneer mangrove species, has adapted to the intertidal habitat along the tropical and subtropical coasts by developing salt glands on its leaf epidermis. Jasmonic acid (JA) is known to regulate the development of various plant epidermis. However, its role in the development of salt glands in <em>A. marina</em> remains unclear. In this study, we treated <em>A. marina</em> seedling using exogenous methyl jasmonate (MeJA) to investigate the effect of JA on the development and cell fate determination of salt glands, stomata and trichomes in <em>A. marina</em> leaf. The results showed MeJA significantly increased both the density of salt glands and the Na⁺ secretion. Besides, MeJA treatment positively regulated the trichome initiation and negatively affected stomatal lineage ground cells, with a significant decrease in stomatal density but no significant change in trichome density, while it exhibited that salt gland cells may partially originate from trichomes or stomatal lineage cells. Moreover, qRT-PCR results indicated that MeJA affects salt gland development via influencing the process of cell cycle, like reducing endoreduplication. These findings clarify how salt glands contribute to <em>A. marina</em> adaptation to coastal intertidal habitat from a tissue development perspective.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113019"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146126369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GmIFS interacts with GmNFR1α and plays a positive role in soybean legume-rhizobia symbiosis GmIFS与GmNFR1α相互作用，在大豆豆科植物与根瘤菌共生中发挥积极作用。

IF 4.1 2区生物学

Plant Science Pub Date : 2026-04-01 Epub Date: 2026-01-29 DOI: 10.1016/j.plantsci.2026.113011

Danxia Ke , Shibo Hou , Zhaoyuan Zhou

{"title":"GmIFS interacts with GmNFR1α and plays a positive role in soybean legume-rhizobia symbiosis","authors":"Danxia Ke , Shibo Hou , Zhaoyuan Zhou","doi":"10.1016/j.plantsci.2026.113011","DOIUrl":"10.1016/j.plantsci.2026.113011","url":null,"abstract":"<div><div>Soybean (<em>Glycine max</em>) serves as a vital source of plant protein and edible oil, while also functioning as a key soil-enriching crop. Symbiotic nitrogen fixation between soybean and rhizobia is crucial for sustainable green agriculture. Nod factor (NF) is a signaling molecule for the establishment of a symbiotic relationship between rhizobia and soybean. The soybean NF receptor GmNFR1α plays a pivotal role in nodulation; however, its signaling pathway remains incompletely characterized. In previous studies, using GmNFR1α as bait, we identified the isoflavone synthase GmIFS2 as an interactor through screening a soybean root/nodule yeast AD-cDNA library. Yeast two-hybrid, luciferase complementation imaging (LCI) in tobacco, and in vitro pull-down assays confirmed the interaction between GmIFS2 and the kinase domain of GmNFR1α (GmNFR1α-pk). Symbiotic phenotyping revealed that the <em>ifs1</em>/<em>2</em> double mutant significantly inhibited the infection process of rhizobia, leading to a remarkable reduction in the number of soybean nodules and shoot/root dry weights. Integrated transcriptomic and metabolomic analyses of roots and nodules from <em>ifs1</em>/<em>2</em> versus wild-type plants demonstrated substantial alterations in genes related to isoflavonoid synthesis, plant-pathogen interactions, and MAPK signaling pathways, alongside significant changes in key enzymes, transcription factors, and metabolites within isoflavonoid and nitrogen metabolism pathways. The study demonstrates that GmNFR1α can directly form a heteromeric complex with the soybean isoflavone synthase GmIFS2, positively regulating symbiotic nodulation between soybeans and rhizobia. The research findings further complement and elucidate the nodulation signaling pathway mediated by GmNFR1α, providing new molecular evidence for the symbiotic interaction mechanism between soybeans and rhizobia.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113011"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146096884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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