Plant Science最新文献

{"title":"OsPP2C03 positively regulates rice drought stress resistance through a non-canonical ABA pathway","authors":"Yao Wang ,&nbsp;Mingxin Chen ,&nbsp;Di Wu,&nbsp;Ru Li,&nbsp;Hanxing Wei,&nbsp;Lingwen Meng,&nbsp;Xuemei Qin,&nbsp;Jinliang Sun,&nbsp;Tianmin Ouyang,&nbsp;Weijian Cen,&nbsp;Rongbai Li,&nbsp;Jijing Luo","doi":"10.1016/j.plantsci.2025.112772","DOIUrl":"10.1016/j.plantsci.2025.112772","url":null,"abstract":"<div><div>Drought is a major factor limiting rice (<em>Oryza sativa</em> L.) production, making the identification of drought-related genes crucial for enhancing drought resistance in rice breeding. The Protein Phosphatase 2 C (PP2C) family, comprising serine/threonine protein phosphatases, plays critical roles in hormonal signaling pathways that orchestrate plant responses to biotic and abiotic stresses. Here, we identified <em>OsPP2C03</em> (<em>LOC_Os01g32964</em>), an H-clade PP2C gene, as a drought-tolerance candidate through genome-wide association studies (GWAS). Phenotypic characterization reveals that disruption of <em>OsPP2C03</em> significantly attenuated drought tolerance in rice. Mechanistic investigations demonstrate that <em>OsPP2C03</em> modulates abscisic acid (ABA)-mediated signaling pathways by influencing stomatal aperture regulation. We further found that under osmotic stress, <em>OsPP2C03</em> translocated from the nucleus to the cytoplasm. Yeast one-hybrid and LUC assays reveal <em>OsORR2</em> functions as an upstream transcriptional regulator of <em>OsPP2C03</em>. Given that it has established the role of <em>OsORR2</em> in salt stress adaptation, we propose that <em>OsPP2C03</em> is also involved in osmotic stress-related signaling networks. These findings provide insights for further elucidating the <em>OsPP2C03</em>-mediated mechanisms underlying drought response in rice.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112772"},"PeriodicalIF":4.1,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mediator subunit 16 positively modulates rice root development by associating with auxin response factor 10","authors":"Yichen Mao ,&nbsp;Luyao Wang ,&nbsp;Jiyuan Li ,&nbsp;Yixuan Fan ,&nbsp;Peng Li ,&nbsp;Chanjuan Mao","doi":"10.1016/j.plantsci.2025.112773","DOIUrl":"10.1016/j.plantsci.2025.112773","url":null,"abstract":"<div><div>The root system is important for plant growth and yield. The Mediator (MED) complex plays a key role in the recruitment and assembly of the transcription machinery for the control of gene expression. However, little information is currently available regarding Mediator-regulated root development in rice. Here, we identified a Mediator subunit OsMED16, the loss of function mutation in which resulted in weakened root system, with less crown roots and lighter root weigh. The yield per plant of <em>osmed16</em> also significantly decreased compared with the wild type. Additionally, OsMED16 alters the transcription of genes related to the auxin response pathway, and physically associates with auxin response factor OsARF10. Moreover, OsARF10 binds directly to promoter of crown root initiation-related gene <em>OsCrl5</em>. Interestingly, OsMED16 is required for the recruitment of RNAPII to <em>OsCrl5</em>. Our finding provides a new insight into the molecular action of mediator subunit in root development.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112773"},"PeriodicalIF":4.1,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the GST family in Cerasus humilis and functional characteristics of ChGSTF5","authors":"Xiaolong Ji ,&nbsp;Xinyu Ye ,&nbsp;Ke Cheng ,&nbsp;Huiping Zhao ,&nbsp;Fan Yang","doi":"10.1016/j.plantsci.2025.112771","DOIUrl":"10.1016/j.plantsci.2025.112771","url":null,"abstract":"<div><div>The anthocyanins are produced in the cytoplasm and are then transported to vacuoles by transport proteins like glutathione S-transferase (GST) to accumulate. <em>Cerasus humilis</em> is a unique fruit tree native to China, characterized by its high anthocyanin content in the fruit. However, no reports have been published regarding GST associated with anthocyanins in <em>C. humilis</em>. This study identified 47 ChGSTs from <em>C. humilis</em>, which were classified into 6 subfamilies. Their motifs, structure, collinearity, and <em>cis</em>-acting elements were thoroughly examined. Based on the transcriptome data from the fruit of <em>C. humilis</em> at various developmental phases, the expression profiles of 47 <em>ChGSTs</em> were analyzed. <em>ChGSTF5</em> expression was significantly upregulated during fruit development. Further research showed that ChGSTF5 was located in the endoplasmic reticulum (ER), and its overexpression encourages the accumulation of anthocyanins in tomato. GUS detection, Y1H assay, and Dual-LUC assay verified that ChMYB1 bound to the <em>ChGSTF5</em> promoter and activated its expression. These results suggest that <em>ChGSTF5</em> may act as a transporter protein directly involved in anthocyanin translocation from the ER to vacuoles, serving as a candidate gene for regulating anthocyanin biosynthesis in <em>C. humilis</em> fruit.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112771"},"PeriodicalIF":4.1,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and structural basis for nitrosoglutathione-dependent redox regulation of triosephosphate isomerase from Chlamydomonas reinhardtii","authors":"Maria Meloni ,&nbsp;Edoardo Jun Mattioli ,&nbsp;Silvia Fanti ,&nbsp;Ginevra Marie Eloise Peppi ,&nbsp;Tancredi Bin ,&nbsp;Giuseppe Gabellini ,&nbsp;Daniele Tedesco ,&nbsp;Julien Henri ,&nbsp;Paolo Trost ,&nbsp;Stéphane D. Lemaire ,&nbsp;Matteo Calvaresi ,&nbsp;Simona Fermani ,&nbsp;Mirko Zaffagnini","doi":"10.1016/j.plantsci.2025.112768","DOIUrl":"10.1016/j.plantsci.2025.112768","url":null,"abstract":"<div><div>Protein <em>S</em>-nitrosylation is a reversible redox-based post-translational modification that plays an important role in cell signaling by modulating protein function and stability. At the molecular level, <em>S</em>-nitrosylation consists of the formation of a nitrosothiol (-SNO) and is primarily induced by the trans-nitrosylating agent nitrosoglutathione (GSNO). Triosephosphate isomerase (TPI), which catalyzes the interconversion of dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, has been identified as a putative target of <em>S</em>-nitrosylation in both plant and non-plant systems. Here we investigate the molecular basis for GSNO-dependent regulation of chloroplast TPI from the model green alga <em>Chlamydomonas reinhardtii</em> (CrTPI). Molecular modelling identified Cys14 and Cys219 as potential sites for interaction with GSNO, though crystallography of GSNO-treated CrTPI revealed <em>S</em>-nitrosylation only at Cys14. To disclose GSNO target sites, we generated and characterized Cys-to-Ser variants for Cys14 and Cys219, identifying Cys219 as a key residue mediating the GSNO-dependent modulation of CrTPI activity. Molecular dynamics simulations further revealed the stabilizing interactions of <em>S</em>-nitrosylated cysteines with their local environments. Overall, our results indicate that CrTPI catalysis is modulated by GSNO through a redox-based mechanism involving Cys219, which highlights a conserved regulatory strategy shared with human TPI.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112768"},"PeriodicalIF":4.1,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The HpGIF1-HpABC44 module facilitates heavy metal detoxification via improved ABA sensitivity and ROS scavenging","authors":"Xiangmei Nie ,&nbsp;Ling Xiao ,&nbsp;Xiaowei Cai ,&nbsp;Ju Cai ,&nbsp;Lan Wen ,&nbsp;Chunqiong Shang ,&nbsp;Junrong Li ,&nbsp;Qiandong Hou ,&nbsp;Luonan Shen ,&nbsp;Kun Yang ,&nbsp;Guang Qiao","doi":"10.1016/j.plantsci.2025.112766","DOIUrl":"10.1016/j.plantsci.2025.112766","url":null,"abstract":"<div><div>ATP-binding cassette (ABC) transporters are known for their roles in detoxification. Although their functions have been elucidated in model plants, the homologs in commercial crops remain unevaluated. Presently, 116 <em>HpABC</em>s were identified in pitaya genome, which can be classified into eight subfamilies, ABCA-G and ABCI. Members from the same subfamily share similar exon-intron structures and conserved motifs. Compared with other plants, <em>HpABC</em>s have undergone purifying selection and the expansion has occurred after the divergence between dicots and monocots. Gene Ontology analysis predicted that most HpABCs are located in membrane system serving as transporters powered by ATP hydrolysis. The analysis of <em>HpABC</em> promoters revealed an abundance of phytohormone- and stress-responsive elements. The RT-qPCR of 12 <em>HpABC</em>s suggested the robust elevation of <em>HpABC44</em> by heavy metals (HMs), indicating its extensive role in the detoxification. <em>HpABC44-OE Arabidopsis</em> accumulated fewer reactive oxygen species under HM treatments, which can be partially explained by the inhibited ROS biosynthesis and promoted ROS scavenging in the seedlings. Further data showed that stomata in transgenic lines were hypersensitive to exogenous abscisic acid, suggesting less cation uptake by transpiration stream. Yeast one-hybrid and luciferase reporter assays suggested that the HM-induced HpGIF1(Growth-regulating Factor-Interacting Factor1) activated <em>HpABC44</em> via binding to its promoter. The RT-qPCR showed that <em>HpGIF1</em> was induced by HMs, especially the Pb and Zn, indicating the activation of <em>HpABC44</em> by HMs was mediated by <em>HpGIF1</em>. To conclude, this work deepens our understanding on how plants cope with HM and provides insight into the role of pitaya ABC transporters in detoxification.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112766"},"PeriodicalIF":4.1,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to “Application of Brachypodium genotypes to the analysis of type II resistance to Fusarium head blight (FHB)” [Plant Sci. 272 (2018) 255–266]","authors":"Peisen Su,&nbsp;Xiuxiu Guo,&nbsp;Yanhui Fan,&nbsp;Liang Wang,&nbsp;Guanghui Yu,&nbsp;Wenyang Ge,&nbsp;Lanfei Zhao,&nbsp;Xin Ma,&nbsp;Jiajie Wu,&nbsp;Anfei Li,&nbsp;Hongwei Wang,&nbsp;Lingrang Kong","doi":"10.1016/j.plantsci.2025.112713","DOIUrl":"10.1016/j.plantsci.2025.112713","url":null,"abstract":"","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112713"},"PeriodicalIF":4.1,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of mitogen-activated protein kinase in plants and AtMAPK6's role in heat stress response in Arabidopsis","authors":"Min Jiang ,&nbsp;Pedro García-Caparrós ,&nbsp;Zhengwei Wang ,&nbsp;Yuxin Zhang ,&nbsp;Yiying Liao ,&nbsp;Yiqing Gong ,&nbsp;Changling Zhao ,&nbsp;Guosong Wen","doi":"10.1016/j.plantsci.2025.112767","DOIUrl":"10.1016/j.plantsci.2025.112767","url":null,"abstract":"<div><div>Mitogen-activated protein kinases (MAPKs) are essential components of tightly regulated signaling pathways that play critical roles in various aspects of plant biology, including growth, development, and defense responses. Despite their significance, the evolutionary origins and processes that have shaped their functional diversity across different plant species still require further investigation. This study elucidated the evolutionary history of MAPK homologs, tracing their lineage to green algae and conducting a comprehensive analysis of their evolutionary trajectory. Our results revealed that MAPK6 orthologs emerged as a spermatophyte-specific innovation, marked by specific patterns of motif gain and/or loss, as well as an increased purifying selection pressure. These features reflect their lineage specific adaptations and functional specialization. Synteny analysis revealed that whole-genome duplication (WGD) events occurred independently in the ancestors of monocotyledons and dicotyledons, contributing substantially to the diversification and formation of the major extant plant lineages. Codon usage analysis demonstrated that different species exhibit varying species-specific preferences, with algae and mosses showing a distinct bias toward G3s, C3s, GC3s, and overall GC content, suggesting potential adaptations or optimizations for translational efficiency. Furthermore, the results of weighted gene co-expression network analysis (WGCNA) reported that the lightcyan module was significantly negatively associated with heat stress response. Within this module, <em>Arabidopsis thaliana MAPK6</em> (<em>AthMAPK6,</em> commonly known as <em>AtMAPK6</em>) was identified as a hub gene in the co-expression network. Functional validation using mutant lines demonstrated that <em>AtMAPK6</em> was involved in thermomorphogenesis under high-temperature conditions. Notably, the N-terminal region of <em>AtPIF4</em> was essential for this interaction between these two proteins. This study collectively provides significant insights into the functional role of <em>AtMAPK6</em> in modulating plant responses to heat stress and offers a broader understanding of the evolutionary perspective on the diversification and specialization of MAPK homologs across plant lineages.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112767"},"PeriodicalIF":4.1,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of TGA transcription factors in wheat and the TaTGA13 negatively modulates stripe rust resistance","authors":"Jiajie Liu ,&nbsp;Rui Li ,&nbsp;Mingjie Xiang ,&nbsp;Xunyin Yuan ,&nbsp;Yifan Tian ,&nbsp;Xuanqi Zhang ,&nbsp;Jingyao Wang ,&nbsp;Jianhui Wu ,&nbsp;Qingdong Zeng ,&nbsp;Dejun Han","doi":"10.1016/j.plantsci.2025.112758","DOIUrl":"10.1016/j.plantsci.2025.112758","url":null,"abstract":"<div><div>TGA transcription factors are critical regulators of plant disease resistance, primarily through reactive oxygen species signaling. While extensively studied in model plants, the evolutionary relationships and functional roles of TGA TFs in hexaploid wheat (<em>Triticum aestivum</em>), particularly concerning pathogen resistance, remain poorly characterized. This study conducted a genome-wide identification of wheat TGA genes, identifying 38 <em>TaTGAs</em> phylogenetically classified into four groups. Analyses revealed conserved chromosomal locations, gene structures, motifs, and promoter cis-elements linked to hormones/stress. Expression profiling under <em>Puccinia striiformis</em> f. sp. <em>tritici</em> (<em>Pst</em>) infection identified 12 differentially expressed <em>TaTGAs</em>, with <em>TaTGA13</em> significantly upregulated. Functional validation using virus-induced gene silencing (VIGS) demonstrated that <em>TaTGA13</em> knockdown enhanced stripe rust resistance, identifying it as a negative regulator. This role was further supported by polymorphism and haplotype analysis of genome-wide association study (GWAS) data. Yeast two-hybrid (Y2H) assay confirmed specific protein interactions for <em>TaTGA13</em>. Our findings provide the first comprehensive analysis of the TGA family in wheat, revealing evolutionary conservation and establishing <em>TaTGA13</em> as a susceptibility factor. This work offers a novel target for breeding resistant cultivars and a foundation for mining wheat resistance genes.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112758"},"PeriodicalIF":4.1,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145054995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scent of disease: Fusarium humuli modulates plant volatiles to attract pod bug for enhanced dissemination.","authors":"Rajshree Verma, Kailash Pati Singh Kushwaha, Satya Kumar, Shilpi Rawat","doi":"10.1016/j.plantsci.2025.112757","DOIUrl":"10.1016/j.plantsci.2025.112757","url":null,"abstract":"<p><p>Plant-insect-microbe interactions form a complex and dynamic triad that significantly influences plant health and disease dissemination. Pod rot of black gram (Vigna mungo (L.) Hepper), caused by Fusarium humuli, is a newly emerging and destructive disease. It was first reported in 2023 from Pantnagar, India. Earlier studies showed a strong correlation between pod rot severity and the presence of the pod bug (Clavigralla tomentosicollis). However, the exact role of the insect in disease development was not well established. In this study, we confirm C. tomentosicollis as a vector of F. humuli. Microscopic and molecular studies revealed fungal conidia on the insect's external surfaces. The pathogen was also present in the gut and excreta, but not in the eggs. Vector transmission experiments proved the insect's role in disease spread. Olfactory preference assays showed a strong attraction of C. tomentosicollis towards infected pods and fungal cultures. This was supported by Gas Chromatography-Mass Spectrometry analysis, which identified specific volatile organic compounds responsible for the behavior. These findings highlight how F. humuli manipulates host volatiles to attract its insect vector. The study offers new insights into the ecology of pathogen spread. It also lays the groundwork for sustainable vector-pathogen management strategies in black gram.</p>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":" ","pages":"112757"},"PeriodicalIF":4.1,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Navigating rice culm resilience: High-throughput quantitative trait locus mapping in indica and tropical japonica derived population","authors":"Akshay Mamidi ,&nbsp;Lavuri Krishna ,&nbsp;Jyothi Badri ,&nbsp;Kalyani M. Barbadikar ,&nbsp;Sanjeeva Rao Durbha ,&nbsp;SV Sai Prasad ,&nbsp;Raman Meenakshi Sundaram","doi":"10.1016/j.plantsci.2025.112756","DOIUrl":"10.1016/j.plantsci.2025.112756","url":null,"abstract":"<div><div>Lodging resistance (LR) in rice is a complex trait determined by a plant’s culm strength to withstand bending or breaking force, especially under high input and direct seeded rice (DSR) conditions. Although, the <em>sd1</em> gene provided early gains in LR, it has now become insufficient in modern cultivars with increased biomass and plant height. Deeper understanding of the intricate morphological and anatomical attributes, mathematical relationships and physical principles governing culm structural strength offer promise for enhancing LR. To unravel the genetic and structural basis of LR, we comprehensively phenotyped 353 recombinant inbred lines (RILs) derived from an inter-subspecific cross between Swarna (<em>indica</em>) and IRGC 39111 (tropical <em>japonica</em>), over multiple seasons for 12 culm strength (CS) and seven grain yield (GY) traits. Breaking resistance (BR) as a measure of internode breaking weight (IBW) and section modulus (SM) emerged as primary contributors to LR. Extensive phenotypic evaluations revealed wide variation and high heritability for most CS traits, as reflected in best linear unbiased prediction (BLUP) estimates. Conversely, traits like tiller number exhibited low heritability as evidenced by the marked difference between their genotypic and phenotypic coefficients of variation indicating a strong environmental influence traits. Significant phenotypic correlations between CS and GY traits were observed. Histological analysis of the culm revealed that strong culm RILs possessed compact, thickened sclerenchymatous tissue and larger secondary vascular bundles compared to Swarna. NGS based GBS generated 63,367 SNP markers, and a high-density genetic map comprising 11,098 binned SNPs revealed 36 QTLs, including 15 major effect loci. Two consistent and major-effect QTLs—<em>qCT7</em> (R²-14.49 %; 109 kb) and <em>qIBW7</em> (R²-16.57 %; 33.83 kb) —on chromosome 7 were identified as key regions for LR. <em>In-silico</em> analysis of <em>qCT7</em> revealed putative candidate genes including BRITTLE CULM1 like <em>(OsBC1L 6, 7, 8),</em> Cellulose synthase genes (<em>OsCesA 3, 6, 8</em>) and <em>OsCSLF 1, 2, 9</em> involved in key processes underpinning cell wall thickening. The identification of <em>qCT7</em> and its future cloning hold potential to enhance lodging resistance in rice, particularly under DSR conditions, contributing to the development of climate-resilient, high-yielding cultivars beyond the Green Revolution paradigm.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"362 ","pages":"Article 112756"},"PeriodicalIF":4.1,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}