Plant Science最新文献

{"title":"Co-overexpression of OsSIZ1 and LtRCA in Arabidopsis thaliana improves heat, drought, and salt tolerance","authors":"Thuvaraki Balasubramaniam,&nbsp;Ruvini Mathangadeera,&nbsp;Tharanya Sugumar,&nbsp;Inosha Wijewardene,&nbsp;Li Sun,&nbsp;Jennifer Smith,&nbsp;Guoxin Shen,&nbsp;Hong Zhang","doi":"10.1016/j.plantsci.2025.112536","DOIUrl":"10.1016/j.plantsci.2025.112536","url":null,"abstract":"<div><div>Abiotic stresses such as heat and drought are major constraints to plant growth and development, which result in tremendous decline in agricultural productivity. The concomitant occurrence of these stresses is common in nature and can lead to bigger losses in crop yield. Hence, implementing a rigorous approach to enhance abiotic stress tolerance is urgently needed. Multi-gene stacking through genetic engineering is considered to be an effective method that could increase abiotic stress tolerance. Previously, it was shown that overexpression of <em>OsSIZ1</em> increased heat, drought, and salt tolerance in transgenic plants. It was recently shown that overexpression of <em>LtRCA</em>, a Rubisco activase gene from <em>Larrea tridentata</em>, could increase heat tolerance in Arabidopsis. In this study, we demonstrated that co-overexpression of <em>OsSIZ1</em> and <em>LtRCA</em> significantly enhances abiotic stress tolerance, particularly under combined drought and heat stress conditions. Notably, <em>OsSIZ1/LtRCA</em> co-overexpressing plants produced at least eight times more seeds and significantly greater biomass compared to wild-type plants, outperforming other transgenic plants. Under individual stress conditions, they yielded six times more seeds under drought stress and three times more under heat stress conditions than wild-type plants. These findings highlight the effectiveness of pyramiding beneficial genes as a powerful strategy to confer broad-spectrum stress resilience in plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112536"},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A non-destructive and precise root monitoring system for hydroponic crops using SWIR hyperspectral imaging","authors":"Maria Merin Antony ,&nbsp;C.S. Suchand Sandeep ,&nbsp;K. Keerthi ,&nbsp;Sreekanth Perumbilavil ,&nbsp;M.M. Bijeesh ,&nbsp;Murukeshan Vadakke Matham","doi":"10.1016/j.plantsci.2025.112544","DOIUrl":"10.1016/j.plantsci.2025.112544","url":null,"abstract":"<div><div>Growing smarter sustainable cities call for a moderate increase in urban agriculture to alleviate burden on traditional agricultural lands. In response, vertical hydroponic farms have emerged as a popular solution, efficiently utilizing limited urban space to produce crops. To ensure consistent, year-round crop quality and maximum yield, continuous monitoring of these farms is crucial. In particular, healthy crop roots are vital for plant growth, as they absorb water and nutrients essential for the growth. Monitoring root dimensions, color, water content, and exudation process provides valuable insights into the overall plant health. However, current root monitoring methods are often contact-based, time-consuming, destructive, subjective, and require sample preparation thereby limiting the potential for future automation. Hence, there is a need for a non-contact and non-invasive approach for root health monitoring based on visual features and root exudate quantification. In this context, this research proposes the development of a non-destructive root monitoring system using a short-wave infrared (SWIR) hyperspectral imager. The proposed method can serve as an excellent system to study the root exudation process and associated root characteristics such as root exudation location and type. The research also proposes the use of a new index termed ‘Root Health Index (RHI)’ based on the wavelength-specific spectral mapping, and Spectral Angle Mapper (SAM) classification, for evaluating root health. The developed system is demonstrated to enable timely detection of crop salinity stress where a significant reduction in fresh weight (∼62.5 %) and root length (∼21.5 %) was observed.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112544"},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling molecular mechanisms of iron and zinc dynamics in rice","authors":"Anjali Verma ,&nbsp;Jebi Sudan ,&nbsp;Robinson C. Jose ,&nbsp;Jayram Bagri ,&nbsp;Zafir Ahmad Naik ,&nbsp;Najeebul Rehman Sofi ,&nbsp;Pardeep Kumar Bhardwaj ,&nbsp;Joy K. Roy ,&nbsp;Ashwani Pareek ,&nbsp;Sajad Majeed Zargar","doi":"10.1016/j.plantsci.2025.112543","DOIUrl":"10.1016/j.plantsci.2025.112543","url":null,"abstract":"<div><div>Iron (Fe) and zinc (Zn) are essential micronutrients critical for human health, yet their deficiencies are widespread, particularly in rice-dependent populations. Rice, a staple food for over half the global population, lacks sufficient bioavailable Fe and Zn in its grains, contributing to global malnutrition. This review delves into the molecular mechanisms governing Fe and Zn transport in rice, focusing on gene families such as <em>IRT, YSL, ZIP</em>, and <em>HMA</em>, which regulate uptake, translocation, and storage. These transporters exhibit intricate interactions and crosstalk, influenced by environmental factors and shared pathways, underscoring the complexity of Fe-Zn homeostasis. Biofortification, through genetic engineering and conventional breeding, emerges as a promising solution to address Fe and Zn deficiencies. Genetic strategies include overexpression of ferritin and nicotianamine synthase genes, alongside manipulation of metal transporter genes, to enhance micronutrient accumulation in rice grains. The advanced breeding approaches including marker-assisted selection and quantitative trait loci (QTL) mapping, complement genetic engineering, offering non-transgenic alternatives for micronutrient enhancement. The common challenges such as regulatory barriers, public perception, and trait stability under diverse conditions necessitate interdisciplinary collaboration and technological advancements.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112543"},"PeriodicalIF":4.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of ribosomal protein StRPS5 in mediating resistance of Solanum tuberosum plants to Phytophthora infestans","authors":"Jing Liu,&nbsp;Yaping Hu,&nbsp;Xiaoqing Lu,&nbsp;Jifen Xu,&nbsp;Hongyang Wang,&nbsp;Wei Tang,&nbsp;Canhui Li","doi":"10.1016/j.plantsci.2025.112539","DOIUrl":"10.1016/j.plantsci.2025.112539","url":null,"abstract":"<div><div>Potato late blight caused by <em>Phytophthora infestans</em> is a devastating disease in potato production. Effectors secreted by <em>P. infestans</em> can target plant proteins and disrupt plant immune responses. The research on plant target proteins has mainly focused on ubiquitination, immune-related proteases, MAPK signal transduction pathways, and transcription factors. The question of whether plants possess novel disease resistance-related proteins or pathways remains unanswered. In this study, we identified a potato ribosomal protein, StRPS5, as a target of the <em>P. infestans</em> RxLR effector, Pi16275. Subcellular co-localization of StRPS5 and Pi16275 was observed in the nucleus, cytoplasm and cell membrane of <em>Nicotiana benthamiana</em>. The expression of <em>StRPS5</em> was induced and up-regulated in the early stage of <em>P. infestans</em> infection. Furthermore, transient overexpression of <em>StRPS5</em> in tobacco leaves was observed to inhibit the infection. We also observed a significant accumulation of H₂O₂ at the site of <em>StRPS5</em> overexpression, indicating a role of StRPS5 in promoting the outbreak of reactive oxygen species (ROS) burst after pathogen infection. Silencing of <em>StRPS5</em> in potato exhibited a marked increase in susceptibility towards <em>P. infestans</em>, whereas overexpression of the gene led to an enhancement of disease resistance. As ROS are key signaling molecules in plant immune responses against pathogens, we investigated flg22-triggered ROS accumulation in transgenic potatoes and the results showed that ROS accumulation in <em>StRPS5-</em>silenced leaves was significantly depressed, while the accumulation was increased in <em>StRPS5</em>-overexpressing leaves. Collectively, our findings demonstrate that potato ribosomal protein StRPS5 serves as a target for the <em>P. infestans</em> effector Pi16275 and that StRPS5 positively regulates potato resistance to <em>P. infestans</em> by increasing the accumulation of ROS.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112539"},"PeriodicalIF":4.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaping the future: Unravelling regulators modulating plant architecture for next-generation crops","authors":"Rajib Kumbhakar ,&nbsp;Mayulika Mondal ,&nbsp;Virevol Thakro ,&nbsp;Shailesh Tripathi ,&nbsp;Swarup K. Parida","doi":"10.1016/j.plantsci.2025.112534","DOIUrl":"10.1016/j.plantsci.2025.112534","url":null,"abstract":"<div><div>Plant architecture traits in crops are modulated through intricate interactions of various genetic pathways, which helps them to adapt to diverse environmental conditions. Key developmental pathways involved in forming plant architecture include the <em>LAZY</em>-<em>TAC</em> (Tiller Angle Control) module regulating branch and tiller angle, the <em>CLAVATA</em>-<em>WUSCHEL</em> pathway controlling shoot apical meristem fate and the <em>GID1</em>-<em>DELLA</em> pathway governing plant height and tillering in major food crops. These pathways function in concert to shape the overall architecture of plants, which is essential for optimizing light capture, resource allocation, reproductive success and eventual crop yield enhancement. Presently, plant architecture of modern crops has been shaped especially by artificial selection of natural alleles that target yield traits. Recent advances in <em>CRISPR-Cas</em>-based genome editing and genomics-assisted breeding strategies have enabled precise genetic manipulation of natural alleles in the functionally relevant genes regulating plant architecture traits in crops. This will assist researchers to select and introgress superior natural alleles in popular cultivars strategically for restructuring their desirable plant-types suitable for mechanical harvesting as well as enhancing the crop yield potential.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"358 ","pages":"Article 112534"},"PeriodicalIF":4.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of cytosolic and organellar gene transcripts in wild and cultivated genotypes of pigeon pea due to simulated herbivory","authors":"Swapnilkumar Meshram ,&nbsp;Debajit Das ,&nbsp;Sanjay Singh ,&nbsp;Mamta Bhattacharjee ,&nbsp;Rahul Ishwar Patil ,&nbsp;S. Arunima ,&nbsp;Prakash Jyoti Kalita ,&nbsp;Jagdish Jaba ,&nbsp;Bidyut Kumar Sarmah ,&nbsp;Sumita Acharjee","doi":"10.1016/j.plantsci.2025.112537","DOIUrl":"10.1016/j.plantsci.2025.112537","url":null,"abstract":"<div><div>Pigeon pea (<em>Cajanus cajan</em>), widely grown in India, suffers significant yield losses due to pod borers (<em>Helicoverpa armigera</em> and <em>Maruca vitrata</em>). Therefore, studying the host resistance mechanism is pivotal for crop improvement. In this study, we conducted transcriptome analysis on two wild-type (WT) <em>Cajanus scarabaeoides</em> accessions (ICP-15761 and ICP-15738) having high levels of resistance to pod borers and two cultivated <em>C. cajan</em> genotypes, ICPL-332 (moderately resistant) and ICPL-87 (susceptible), following simulated herbivory with <em>H. armigera</em> oral secretions (OS). Differential gene expression analysis identified 3573 and 4677 differentially expressed genes (DEGs) in ICP-15761 and ICP-15738, whereas 4149 and 3639 DEGs were documented in ICPL-332 and ICPL-87, respectively. Genes related to chloroplast biogenesis, photosynthesis, and chlorophyll metabolism exhibited significant differential expression, indicating chloroplast reprogramming under simulated herbivory. Significant upregulation of key defense genes, including chitinases and cysteine proteases, in <em>C. scarabaeoides</em> accessions highlighted robust defense pathway activation. A genotype-specific shift in transcription factors, phytohormones, and calcium signaling-related gene expression was noted. Higher levels of expression of aspartic proteinases and pathogenesis-related proteins in cultivated genotypes suggesting adaptive evolutionary traits. This is a novel insight on molecular mechanism of defense in a wild type, <em>C. scarabaeoides</em> and cultivated genotypes of pigeon pea under simulated herbivory. The information on cytosolic and organellar gene changes in pigeon pea due to <em>H</em>. <em>armigera</em> OS mediated-simulated herbivory may help develop pigeon pea varieties that are resistant to pod borer infestations.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112537"},"PeriodicalIF":4.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinase domain diversification drives specificity in BRI1 and non-BRI1 RLKs in brassinosteroid signaling","authors":"Khawar Ali ,&nbsp;Wenjuan Li ,&nbsp;Guang Wu","doi":"10.1016/j.plantsci.2025.112531","DOIUrl":"10.1016/j.plantsci.2025.112531","url":null,"abstract":"<div><div>Receptor-like kinases (RLKs) are one of the largest families of Eukaryotic protein kinases (EPKs) that evolved through repeated duplication and diversification events in plants. RLKs regulate diverse roles of plant growth and development. Brassinosteroid Insensitive 1 (BRI1) and its family members BRI1-Like 1 (BRL1/3), BRL2, Excess Microsporocytes 1 (EMS1), and Nematode-Induced LRR-RLK 1 (NILR1) that belong to the LRR-RLK family of RLKs, control distinct biological functions through a conserved brassinosteroid (BR) signaling pathway. We previously demonstrated that the kinase specificity between BRI1 and GASSHO1 (GSO1) is allosterically regulated by merely two subdomains, raising a question of how different RLKs control distinct biological functions through their conserved kinase domain (KD). Here, we engineered chimeric receptors by fusing the extracellular domain (ECD) of BRI1 with KD of the BRI1 family and with non-BRI1 family RLKs, including BAK1-Interacting Receptor-like Kinase 1 (BIR1), BIR2, TOAD2 (RPK2), Barely Any Meristem (BAM1), CLAVATA 1 (CLV1), SOBIR1, Elongation Factor (EF-Tu) Receptor (EFR), Glycan Perception 4 (IGP4), and Strubbelig-Receptor Family 8 (SRF8), and confirmed that only the BRI1 family achieved BR signal output but not the others. We then replaced the S1 and S2 subdomains of the chimeric receptors with the corresponding S1 and S2 subdomains of the BRI1 kinase and found that except GSO1^{<em>BRI1-S1S2</em>}, no other chimeric receptor could induce BR signaling in <em>bri1–301</em> mutants. However, chimeric receptors <em>RPK2</em>^{<em>BRI1-S1(E)S2</em>}, <em>EFR</em>^{<em>BRI1-S1(E)S2</em>}, <em>IGP4</em>^{<em>BRI1-S1(E)S2</em>}, <em>BAM1</em>^{<em>BRI1-S1(E)S2</em>}, and <em>SRF8</em>^{<em>BRI1-S1(E)S2</em>} with an extended S1 subdomain S1(E) of BRI1 not only rescued <em>bri1–301</em>, but also achieved molecular phenotypes. In conclusion, this study provides evidence that signaling specificity of the RLKs has evolved through evolution of the S1 and S2 subdomains.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112531"},"PeriodicalIF":4.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of TaWRKY254 in salt tolerance based on genome-wide analysis of the WRKY gene family in wheat core parent Zhou8425B","authors":"Yuxin Yang ,&nbsp;Huimin Huang ,&nbsp;Zhao Xin ,&nbsp;Chenxi Zhou,&nbsp;Huifang Li,&nbsp;Tongtong Li,&nbsp;Anqi Zhang,&nbsp;Mengquan Cheng,&nbsp;Xiaode Li,&nbsp;Guangwei Li,&nbsp;Kunpu Zhang,&nbsp;Daowen Wang","doi":"10.1016/j.plantsci.2025.112540","DOIUrl":"10.1016/j.plantsci.2025.112540","url":null,"abstract":"<div><div>The WRKY gene family plays a pivotal role in regulating plant growth, development, and stress responses. Zhou8425B, a core wheat parent in Chinese breeding programs known for its superior agronomic traits, remains underexplored in terms of its WRKY functional landscape. In this study, we identified 294 WRKY transcription factors in the Zhou8425B genome and conducted comprehensive bioinformatics analyses covering gene structure, protein properties, phylogenetic relationships, conserved motifs, and <em>cis</em>-regulatory elements. RNA-seq analysis across 12 tissues revealed that 274 WRKY genes are highly expressed and form distinct tissue-specific clusters. Notably, <em>TaWRKY254</em> (TraesZ8425B6B01G167200) was significantly upregulated under various environmental stresses. RT-qPCR confirmed that <em>TaWRKY254</em> expression under salt stress was substantially higher in Zhou8425B compared to Chinese Spring. Sequence diversity analysis revealed a 513 bp deletion in the promoter region and a T-to-C nonsynonymous mutation in the exon, resulting in an isoleucine-to-valine substitution in Zhou8425B. Based on this 513 bp difference, we developed a specific molecular marker and genotyped the recombinant inbred lines (RILs) from a Zhou8425B × Chinese Spring. Phenotypic analysis showed that RILs carrying the <em>TaWRKY254</em>^Zhou8425B genotype exhibited enhanced salt tolerance, as evidenced by increased catalase, proline, and soluble protein levels, reduced lipid peroxidation, and significantly higher thousand kernel weight compared to those with the <em>TaWRKY254</em>^CS genotype. These findings suggest that <em>TaWRKY254</em> may play an important role in salt stress adaptation and yield-related traits, highlighting its potential as a genetic resource for salt-tolerant wheat breeding.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112540"},"PeriodicalIF":4.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABA and defoliation improve phenolic composition of wine grapes while ABA reduces must free amino acid content and modifies its profile","authors":"Johann Martínez-Lüscher ,&nbsp;Andrea Cabodevilla ,&nbsp;Mohammad Abdullah ,&nbsp;Andoni Iglesias ,&nbsp;Ángel Zamarreño ,&nbsp;José María García-Mina ,&nbsp;Fermín Morales ,&nbsp;Nieves Goicoechea ,&nbsp;Inmaculada Pascual","doi":"10.1016/j.plantsci.2025.112541","DOIUrl":"10.1016/j.plantsci.2025.112541","url":null,"abstract":"<div><div>Grape (<em>Vitis vinifera</em> L.) ripening modulation through exogenous growth regulators and cultural practices has benefits but also downfalls. Abscisic acid (ABA) applications have shown to induce great increases of anthocyanin synthesis and wine color. In turn, severe defoliation may reduce translocation of sugars into berries delaying ripening. The aim of the study was to investigate the interactive effects of exogenous ABA applications to berries and 75 % vine defoliation on grape ripening and nano vinification composition of Tempranillo fruit-bearing cuttings at bunch closure, 46 days after fruit set. At bunch closure the ABA applications started, and plants were partially defoliated (from 12 of untreated to down to 3 leaves) in a factorial design 2 by 2. After 48 h of treatment application, ABA levels in berries increased 10-fold, and concomitantly, the indole acetic acid (IAA) in the berries also increased. Grape anthocyanin content was increased by ABA for berries with the same total soluble solids (TSS), and this was translated to an increase in total polyphenol index (A280) of nano vinifications. Another effect of ABA application was a decrease in free amino acids in the berry must. In addition, the profile of amino acids was shifted to higher proportion of GABA and Glu in detriment of Gln, which implies a GABA shunt activation. Defoliation delayed berry development and induced changes in composition, but only titratable acidity was reduced for berries with the same TSS. Nano vinifications from defoliated plants had higher color attributes (free anthocyanins, flavonols, A360, A620, A420/520 and A620/520). When these two treatments were combined, berries had the highest anthocyanin content while amino acids attributed as aromatic precursors were reduced in must. Results suggest that grape and wine quality attributes such as acidity and color intensity are not necessarily related to ripening speed. The impacts of ABA on berry nitrogen metabolism, deserve further investigation.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112541"},"PeriodicalIF":4.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of the Nitraria sibirica Pall. H+-pyrophosphatase gene NsVP1 improves Arabidopsis salt tolerance","authors":"Xihong Wan ,&nbsp;Xiuyan Yang ,&nbsp;Rongfeng Duan ,&nbsp;Rong Li ,&nbsp;Yongxin You ,&nbsp;Huaxin Zhang ,&nbsp;Shuaihui Zhang ,&nbsp;Pengyu Ying ,&nbsp;Huilong Zhang","doi":"10.1016/j.plantsci.2025.112530","DOIUrl":"10.1016/j.plantsci.2025.112530","url":null,"abstract":"<div><div><em>Nitraria sibirica</em> Pall., a perennial euhalophytic dwarf shrub, exhibits exceptional salt tolerance and serves as an ideal model species for saline-alkali land remediation, identification of novel salt-responsive genes, and deciphering molecular mechanisms underlying halophytic adaptation. Our previous investigations have shown that salt stress significantly upregulates both the expression and enzymatic activity of vacuolar H⁺-pyrophosphatase (H⁺-PPase) in this species. However, the detailed functional specificity of H⁺-PPase in <em>N. sibirica</em> remains poorly characterized. Here, we cloned and functionally characterized <em>NsVP1</em>, a tonoplast-localized type I H⁺-PPase from <em>N. sibirica</em>. Quantitative real-time PCR (RT-qPCR) analysis revealed that 400 mM NaCl treatment induced significant upregulation of <em>NsVP1</em> expression, resulting in 6.6-fold and 29.7-fold increases in stems and leaves, respectively. Functional characterization studies demonstrated that <em>NsVP1</em> overexpression in Arabidopsis conferred enhanced salinity tolerance through multifaceted regulatory mechanisms: (1) promoted vacuolar compartmentalization and Na⁺ exclusion via upregulated vacuolar H⁺-PPase activity and synergistic interactions with NHX1 and SOS1, (2) decreased K⁺ loss and maintained cytosolic K⁺/Na⁺ homeostasis, and (3) improved reactive oxygen species scavenging capacity. Notably, the succulent stem and leaf tissues of <em>N. sibirica</em> may enhance its ability to compartmentalize Na⁺, contributing to its superior salt tolerance.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"357 ","pages":"Article 112530"},"PeriodicalIF":4.2,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}