Plant Science最新文献_第7页

Synthetic elicitors-induced defense in crops against herbivory: A review 合成诱导剂诱导的作物草食防御研究进展。

IF 4.2 2区生物学

Plant Science Pub Date : 2025-01-08 DOI: 10.1016/j.plantsci.2025.112387

Malawanthkar Rani, Ramasamy Kanagaraj Murali-Baskaran

引用次数: 0

Retraction notice to “Scorpion peptide LqhIT2 activates phenylpropanoid pathways via jasmonate to increase rice resistance to rice leafrollers” [Plant Sci. 230 (2015) 1–11]

IF 4.2 2区生物学

Plant Science Pub Date : 2025-01-07 DOI: 10.1016/j.plantsci.2024.112372

Xiuzi Tianpei , Dong Li , Ping Qiu , Jie Luo , Yingguo Zhu , Shaoqing Li

引用次数: 0

Overexpression of SikPsaF can increase the biomass of Broussonetia papyrifera by improving its photosynthetic efficiency and cold tolerance SikPsaF过表达可以通过提高纸莎草的光合效率和耐寒性来增加其生物量。

IF 4.2 2区生物学

Plant Science Pub Date : 2025-01-03 DOI: 10.1016/j.plantsci.2024.112380

Mei Wang , Xiangxue Yu , Jingyi Zhao , Zhijia Tian , Bo Chen , Qian Li , Dingguo Zhang , Fanfan Zhang , Li Zhang , Xinyong Guo

{"title":"Overexpression of SikPsaF can increase the biomass of Broussonetia papyrifera by improving its photosynthetic efficiency and cold tolerance","authors":"Mei Wang , Xiangxue Yu , Jingyi Zhao , Zhijia Tian , Bo Chen , Qian Li , Dingguo Zhang , Fanfan Zhang , Li Zhang , Xinyong Guo","doi":"10.1016/j.plantsci.2024.112380","DOIUrl":"10.1016/j.plantsci.2024.112380","url":null,"abstract":"<div><div>Photosynthesis is essential for the accumulation of organic compounds in plant leaves. Study of photosynthesis in the leaves of <em>Broussonetia papyrifera</em> is crucial for enhancing its biomass production, growth, and development. Here, we cloned the <em>SikPsaF</em> gene associated with photosynthesis from <em>Saussurea involucrata</em> and constructed a vector that was introduced into <em>B. papyrifera</em> to generate a transgenic strain. We then assessed various photosynthesis-related parameters in the transgenic plants and examined the function of this gene and its expression patterns under cold stress. The results showed that <em>SikPsaF</em> was localized to chloroplasts. Its expression was induced by light, and its expression was higher in the leaves than in other tissues. Furthermore, <em>SikPsaF</em> expression increased significantly under cold stress. The biomass of transgenic lines was greater than that of wild-type plants. Overexpression of this gene led to increases in the chlorophyll content and photosynthetic indices, which mitigated cell membrane damage and reduced reactive oxygen species (ROS) accumulation. <em>SikPsaF</em> overexpression also helped maintain high antioxidant enzyme activity and a high content of osmoregulatory substances during stress; the increased enzyme activities were due to up-regulated gene expression. Overexpression of <em>SikPsaF</em> has a major effect on growth and development by enhancing photosynthetic efficiency, improving yield, conferring cold resistance, and reducing damage to the cell membrane and ROS accumulation at low temperatures. In summary, our findings indicate that these transgenic plants have enhanced photosynthetic efficiency and resilience against biotic stresses.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112380"},"PeriodicalIF":4.2,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of ClAPRR2, a key candidate gene controlling watermelon stripe color 西瓜条纹色关键候选基因ClAPRR2的鉴定与特性研究

IF 4.2 2区生物学

Plant Science Pub Date : 2025-01-03 DOI: 10.1016/j.plantsci.2024.112383

Shuang Liang , Miaomiao Yang , Linlin Zhang , Xufeng Fang , Xian Zhang , Chunhua Wei , Zuyun Dai , Zhongzhou Yang , Chaonan Wang , Bin Liu , Feishi Luan , Shi Liu

{"title":"Identification and characterization of ClAPRR2, a key candidate gene controlling watermelon stripe color","authors":"Shuang Liang , Miaomiao Yang , Linlin Zhang , Xufeng Fang , Xian Zhang , Chunhua Wei , Zuyun Dai , Zhongzhou Yang , Chaonan Wang , Bin Liu , Feishi Luan , Shi Liu","doi":"10.1016/j.plantsci.2024.112383","DOIUrl":"10.1016/j.plantsci.2024.112383","url":null,"abstract":"<div><div>The stripe color of watermelon is a vital commercial trait and is the focus of attention of consumers and researchers. However, the genetic determinants of watermelon stripe color are incompletely understood. Based on the results of preliminary localization studies, we constructed a large-capacity F<sub>2</sub> generation population (710 plants) using light-green striped ZXG1555 and green-striped Cream of Saskatchewan (COS) watermelon strains as parental lines for fine mapping. Genes controlling stripe color were located in an 85.284 kb region on chromosome 9, which contained five candidate genes. Combined with parental phenotypes, chlorophyll contents of rinds and stripes were assayed. Gene sequence alignment and transcriptional level analysis of parental lines predicted <em>Cla97C09G175170</em> (encoding a two-component response regulator-like protein, <em>APRR2</em>) as the best candidate gene for stripe color trait. Two SNPs in the <em>ClAPRR2</em> coding region caused amino acid substitutions, but were not located in the conserved domain, while a 12 bp insertion caused premature translation termination and a 35 amino acid deletion in the conserved domain and may have affected <em>ClAPRR2</em> function in ZXG1555. Subcellular localization analysis showed that <em>ClAPRR2</em> was expressed in the ZXG1555 cell membrane but was located in the nucleus and cell membrane of COS. Nucleotide polymorphisms and deletions were also detected in the promoter region between parental lines and caused cis-acting element variations. Luciferase activity suggested that promoter variations may not be the main factor in the regulation of <em>ClAPRR2</em> expression.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112383"},"PeriodicalIF":4.2,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights of advanced biotechnological engineering strategies for tanshinone biosynthesis in Salvia miltiorrhiza 丹参酮生物合成先进生物技术工程策略的新见解。

IF 4.2 2区生物学

Plant Science Pub Date : 2025-01-03 DOI: 10.1016/j.plantsci.2025.112384

Minyu Shou , Qinzhe Lin , Ying Xu , Ruiyan Zhu , Min Shi , Guoyin Kai

引用次数: 0

Dissecting sequence-structure-function-diversity in plant cryptochromes 植物隐色素序列-结构-功能-多样性分析。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-12-31 DOI: 10.1016/j.plantsci.2024.112381

Pratichi Sarkar , Aparna Boral , Devrani Mitra

{"title":"Dissecting sequence-structure-function-diversity in plant cryptochromes","authors":"Pratichi Sarkar , Aparna Boral , Devrani Mitra","doi":"10.1016/j.plantsci.2024.112381","DOIUrl":"10.1016/j.plantsci.2024.112381","url":null,"abstract":"<div><div>Ubiquitous to every stratum of life, cryptochromes regulate numerous light dependent functions in terrestrial plants. These include light-dependent transcription, circadian rhythm, inhibition of hypocotyl elongation, programmed cell death, promotion of floral initiation, mediation of gravitropic response, responding to biotic and abiotic stress etc. There have been quite a few seminal reviews including on plant cryptochromes, focusing mostly on the detailed functional aspects. This review primarily focuses on understanding the link connecting sequence-structure hierarchy behind the functional diversity in plant cryptochromes. With available sequence information and 3D structure data, we hereby explore the molecular origin of functional diversity in both the subtypes i.e., CRY1 and CRY2. First, we discuss the structural details and functional distinctiveness of all subtypes of plant cryptochromes. Next we draw a comparison not just between two cryptochromes but also other Cryptochrome/Photolyase Family (CPF) members e.g. CRY-DASH/CRY3 and CPD/6–4 photolyases of plant origin. Further, by constructing a phylogenetic profile from multiple sequence alignment we investigate how a crucial activity like DNA repair is restricted to some members of CPF and not all. It is a well-known fact that the function of a protein is heavily if not solely guided by the structure-sequence relationship. Therefore, the resultant hypothesis as drawn from this comparative and collective study could predict functions of many under-studied plant cryptochromes when compared with their well-studied counterparts like <em>Arabidopsis</em> cryptochromes. An extensive sequence-structure-function analysis complemented with evolutionary studies and bibliographic survey is useful towards understanding the immensely diverse CPF.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112381"},"PeriodicalIF":4.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142922544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of advanced omics tools and resources for the improvement of industrial oil crops 经济油料作物改良的先进组学工具和资源探索。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-12-31 DOI: 10.1016/j.plantsci.2024.112367

Badal Mahakalkar , Virender Kumar , Sreeja Sudhakaran , Vandana Thakral , Sanskriti Vats , Rushil Mandlik , Rupesh Deshmukh , Tilak Raj Sharma , Humira Sonah

{"title":"Exploration of advanced omics tools and resources for the improvement of industrial oil crops","authors":"Badal Mahakalkar , Virender Kumar , Sreeja Sudhakaran , Vandana Thakral , Sanskriti Vats , Rushil Mandlik , Rupesh Deshmukh , Tilak Raj Sharma , Humira Sonah","doi":"10.1016/j.plantsci.2024.112367","DOIUrl":"10.1016/j.plantsci.2024.112367","url":null,"abstract":"<div><div>The rapid advancement in the field of omics approaches plays a crucial role in the development of improved industrial oil crops. Industrial oil crops are important for many sectors like food processing, biofuels, cosmetics, and pharmaceuticals, making them indispensable contributors to global economies and these crops serve as vital elements in a multitude of industrial processes. Significant improvements in genomics have revolutionized the agricultural sector, particularly in the realm of oil crops. Cutting-edge advancements have facilitated the efficient sequencing of genomes for key commercial oil crops. This breakthrough not only enhances our understanding of the genetic makeup of these crops but also empowers breeders with invaluable insights for targeted genetic manipulation and breeding programs. Moreover, integrating transcriptomics with genomic data has assisted in a new era of precision agriculture. This approach provides an in-depth understanding of molecular mechanisms involved in traits of interest, such as oil content, yield potential, and resistance to biotic and abiotic stresses. Proteomics methods are instrumental in deciphering the intricacies of protein structure, interactions, and function, while metabolomics and ionomics shed light on the intricate network of metabolites and ions within biological systems. Each omics discipline offers unique insights, and their integration holds the promise of enriching our understanding and furnishing invaluable insights for enhancing oil crops. This review delves into the efficacy and constraints of various omics approaches in the context of refining industrial oil crops. Moreover, it underscores the importance of multi-omics strategies and explores their convergence with genetic engineering techniques to cultivate superior oil crop varieties.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112367"},"PeriodicalIF":4.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142922546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification of the WOX gene family in Populus davidiana×P.bolleana and functional analysis of PdbWOX4 in salt resistance 杨树WOX基因家族的全基因组鉴定davidiana×P。PdbWOX4在抗盐中的功能分析。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-12-28 DOI: 10.1016/j.plantsci.2024.112379

Zhengyang Li , Ziqian Zhang , Yumeng Xu , Xiaojin Lei , Qinjun Xie , Zhongyuan Liu , Yanmin Wang , Caiqiu Gao

{"title":"Genome-wide identification of the WOX gene family in Populus davidiana×P.bolleana and functional analysis of PdbWOX4 in salt resistance","authors":"Zhengyang Li , Ziqian Zhang , Yumeng Xu , Xiaojin Lei , Qinjun Xie , Zhongyuan Liu , Yanmin Wang , Caiqiu Gao","doi":"10.1016/j.plantsci.2024.112379","DOIUrl":"10.1016/j.plantsci.2024.112379","url":null,"abstract":"<div><div>WOX transcription factors (TFs) are plant specific transcription regulatory factors that have a momentous role in maintaining plant growth and development and responding to abiotic stress. In this study, a total of 13 <em>PdbWOX</em> genes were identified. qRT-PCR analyses showed that 13 <em>PdbWOX</em> genes were responsive to salt stress. Notably, the expression of <em>PdbWOX4</em> was significantly changed at all time points under NaCl stress, suggesting that <em>PdbWOX4</em> expression may be involved in salt stress. Further, an overexpression vector of <em>PdbWOX4</em> was constructed and transient transformed into Shanxin poplar. Biochemical staining and physiological parameter analysis showed that overexpression of <em>PdbWOX4</em> decreased the total antioxidant capacity (T-AOC) and peroxidase (POD) activity, which in turn reduced the scavenging capacity of reactive oxygen species (ROS), and increased the cell damage and death induced by salt stress. qRT-PCR and ChIP-PCR demonstrated that PdbWOX4 can regulate the expression of <em>PdbDREB2C</em> by binding to its promoter. Further analyses revealed that overexpression of <em>PdbDREB2C</em> can reduce cellular damage by increasing ROS scavenging capacity thereby improving salt tolerance in Shanxin poplar. Taken together, we found that <em>PdbWOX4</em> negatively regulated the salt tolerance of Shanxin poplar by repressing the <em>PdbDREB2C</em>, suggesting that <em>PdbWOX4</em> may play a key role in the tolerance of Shanxin poplar to salt stress, and is an important candidate gene for molecular resistance breeding in forest trees.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112379"},"PeriodicalIF":4.2,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide study of the R2R3-MYB gene family and analysis of HhMYB111r-induced salt tolerance in Hibiscus hamabo Sieb. et Zucc 木芙蓉R2R3-MYB基因家族的全基因组研究及hhmyb111r诱导的耐盐性分析调查。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-12-27 DOI: 10.1016/j.plantsci.2024.112378

Yu Xu , Longjie Ni , Chaoguang Yu , Jianfeng Hua , Yunlong Yin , Chunsun Gu , Zhiquan Wang

{"title":"Genome-wide study of the R2R3-MYB gene family and analysis of HhMYB111r-induced salt tolerance in Hibiscus hamabo Sieb. et Zucc","authors":"Yu Xu , Longjie Ni , Chaoguang Yu , Jianfeng Hua , Yunlong Yin , Chunsun Gu , Zhiquan Wang","doi":"10.1016/j.plantsci.2024.112378","DOIUrl":"10.1016/j.plantsci.2024.112378","url":null,"abstract":"<div><div><em>Hibiscus hamabo</em> Sieb. et Zucc. (<em>H. hamabo</em>) is a semi-mangrove plant with excellent stress tolerance that plays a crucial role in the ecological restoration of saline and alkaline areas. It is an ideal candidate species for studying the mechanisms involved in stress tolerance. Although the MYB gene family has preliminarily been characterized in <em>H. hamabo</em>, the specific functions and action mechanisms of the R2R3-MYB genes in this species have not fully been elucidated. In this study, 190 R2R3-MYB genes were identified at the genomic level using bioinformatics methods. The genes were divided into 26 subgroups based on their evolutionary relationships and found to be distributed randomly on 46 chromosomes. RNA sequencing data and subsequent real-time quantitative PCR analysis of 12 differentially expressed R2R3-HhMYB genes showed <em>HhMYB111r</em> to be highly expressed under various abiotic stress conditions. Self-activation and subcellular localization results showed that the intact HhMYB111r had strong self-activation activity and located in both the nucleus and cytoplasm. Overexpression in <em>Arabidopsis</em> significantly improved salt tolerance, and silencing <em>HhMYB111r</em> reduced the tolerance of <em>H. hamabo</em> to salt stress, indicating that <em>HhMYB111r</em> positively regulates the salt stress response. In this first analysis of the R2R3-MYB gene family in <em>H. hamabo</em>, we identified a key salt stress response gene, <em>HhMYB111r</em>, enriching the understanding of MYB function and laying a foundation for exploring the abiotic stress response of plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112378"},"PeriodicalIF":4.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new method for identifying proteins involved in DNA methylation through reverse genetics in Arabidopsis 通过反向遗传学鉴定拟南芥DNA甲基化相关蛋白的新方法。

IF 4.2 2区生物学

Plant Science Pub Date : 2024-12-27 DOI: 10.1016/j.plantsci.2024.112376

Wei Miao , Jie Dai , Li Zhang , Zhile Liang , Xiaoxuan Sun , Meizi Huang , Aqin Zhang , Long Zheng , Yongjun Li , Ying Li

{"title":"A new method for identifying proteins involved in DNA methylation through reverse genetics in Arabidopsis","authors":"Wei Miao , Jie Dai , Li Zhang , Zhile Liang , Xiaoxuan Sun , Meizi Huang , Aqin Zhang , Long Zheng , Yongjun Li , Ying Li","doi":"10.1016/j.plantsci.2024.112376","DOIUrl":"10.1016/j.plantsci.2024.112376","url":null,"abstract":"<div><div>Forward genetic screens have uncovered numerous genes involved in DNA methylation regulation, but these methods are often time-intensive, costly, and labor-intensive. To address these limitations, this study utilized CRISPR technology to knockout selected co-expressed genes, enabling the rapid identification of low luciferase (LUC) luminescence mutants in the Col-LUC line, which harbors a LUC transgene driven by a 2 × 35S promoter in <em>Arabidopsis</em>. As proof of concept, the <em>repressor of silencing 1</em> (<em>ROS1</em>) and <em>RNA-directed DNA methylation 1</em> (<em>RDM1</em>) genes were used as controls, while the <em>increased DNA methylation 3</em> (<em>IDM3</em>) gene, co-expressed with <em>ROS1</em>, was selected as the target for gene knockout experiments. The results demonstrated that combining co-expression analysis with CRISPR technology is an effective strategy for generating low LUC luminescence mutants in the Col-LUC line. Notably, a new mutant, named <em>reduced luminescence 1</em> (<em>rl1</em>), was identified through this approach. The <em>rl1</em> mutant exhibited genome-wide DNA hypermethylation, and its reduced luminescence phenotype was largely reversed by treatment with the DNA methylation inhibitor 5-Aza-2’-deoxycytidine, confirming its anti-silencing role in DNA methylation regulation. This study presents a novel and efficient approach for obtaining low luminescence mutants in the Col-LUC line and identifies RL1 as a previously uncharacterized protein involved in DNA methylation regulation.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112376"},"PeriodicalIF":4.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0