Trans-Zeatin Riboside enhanced plant regeneration and Auxins induced adventitious rooting in Stevia rebaudiana

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-09-25 DOI:10.1016/j.plantsci.2025.112788

Mohammad Aqa Mohammadi , Kecai Chen , Weicai Song , Liping Zhu , Jin Gao , Heng Yin

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引用次数: 0

Abstract

Stevia rebaudiana is an economically important crop prized for its natural sweeteners. This study investigated the effects of trans-zeatin riboside (tZR) on plant regeneration in vertical and horizontal orientations, different Murashige and Skoog (MS) medium strengths concentration, and various auxins on adventitious root formation in Stevia rebaudiana cuttings. Direct organogenesis enabled efficient shoot regeneration, with the highest shoot proliferation rate (10.5 shoots/explant) achieved using 5 mg/L tZR in horizontal orientation after three weeks. Gibberellic acid (GA₃) further enhanced shoot elongation with a low concentration of GA₃ (0.5 mg/L) increased plant height, whereas a high GA₃ concentration cause abnormal growth and inhibit rooting. For root development, half-strength MS medium was optimal for rooting of cuttings, and half-strength MS with 1.0 mg/L α-naphthalene acetic acid (NAA) yielded the longest roots, while 1.0 mg/L indole-3-butyric acid (IBA) producing the highest root numbers and highest transplant survival rates compare to other auxins concentrations. Notably, adventitious roots also formed on hormone-free medium before successful soil acclimatization. This research establishes an efficient in vitro regeneration system for Stevia rebaudiana, offering practical applications for commercial propagation and biotechnological advancements.

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反式玉米蛋白核苷促进甜菊植株再生，生长素诱导不定根

甜菊糖是一种重要的经济作物，因其天然甜味剂而备受推崇。研究了反式玉米蛋白核苷（tZR）对甜菊扦插植株垂直和水平方向再生、不同Murashige和Skoog （MS）培养基强度浓度以及不同生长素对甜菊扦插不定根形成的影响。3周后，在5 mg/L tZR水平培养条件下，芽增殖率最高（10.5个芽/外植体）。低浓度GA3（0.5 mg/L）可提高植株的株高，高浓度的GA3可导致植株生长异常，抑制生根。在根系发育方面，半强度MS培养基最适合插枝生根，其中1.0 mg/L α-萘乙酸（NAA）培养基的根最长，1.0 mg/L吲哚-3-丁酸（IBA）培养基的根数最多，移栽成活率最高。值得注意的是，在土壤驯化成功之前，不定根也在无激素培养基上形成。本研究建立了一种高效的甜菊糖体外再生体系，为甜菊糖的商业化繁殖和生物技术进步提供了实际应用。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.