Plant Molecular Biology Reporter最新文献

{"title":"Molecular Diversity Studies and Core Development in Sesame Germplasm (Sesamum indicum L.) Using SSR Markers","authors":"Rasna Maurya, Shivani Singh, Yangala Sudheer Babu, Fatima Nazish Khan, Bhagwat Nawade, Harinder Vishwakarma, Ajay Kumar, Rashmi Yadav, Radhamani Jalli, Mahalingam Angamuthu, Mothilal Alagirisamy, Rajkumar Subramani, Senthilraja Govindasamy, Ashok Kumar, Kuldeep Singh, Parimalan Rangan","doi":"10.1007/s11105-024-01476-z","DOIUrl":"https://doi.org/10.1007/s11105-024-01476-z","url":null,"abstract":"<p>Sesame (<i>Sesamum indicum</i> L.), an ancient oilseed crop being cultivated across geographical locations in the tropics, is known for its high-quality oil with a longer shelf life. India, being the center of diversity for this crop, understanding the genetic variability of sesame germplasm being conserved in the national Genebank (NGB) of ICAR-NBPGR, will help identify genotypes for its potential use in broadening the genetic base of the cultivars for sesame crop improvement. We report here the molecular diversity analysis performed using SSR markers on a set of 2,496 sesame germplasm. Hence, the derived data was also subjected to population structure analysis, and a molecular core was generated to assess its phenotypic variability. Parallelly, they were phenotypically characterized for important qualitative and quantitative traits as per the standard descriptor developed by IPGRI, and accessions exhibiting desirable traits were identified. The sesame germplasm used in our study represents collections from 17 countries across the globe and 26 states in India. A total of 140 alleles were obtained using seven polymorphic SSR markers selected from an initial screening comprising 43 SSR markers. The observed heterozygosity was less than the expected heterozygosity since it is a self-pollinated crop (up to 35% outcrossing is reported, categorized as often cross-pollinated). The molecular diversity analysis grouped 2496 accessions into six clusters, while the population structure analysis grouped them into three major clusters or populations. A molecular core developed using the PowerCore software identified 196 accessions, representing all the alleles from the entire 2496 accessions, that can be utilized in breeding programs after phenotypic validation. This study contributes to genetic diversity assessment for sesame germplasm, identifying genetically diverse accessions, and establishing a core set that encapsulates the genetic variability of the sesame germplasm collection. These findings hold relevance for addressing agricultural challenges and enhancing the resilience and productivity of sesame crops in various environmental conditions.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141784761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Biostimulant Potential of the Seaweeds Galaxaura oblongata and Turbenaria ornate in Improving Drought Tolerance in Rice Plant (Oryza sativa L.)","authors":"Rana M. Alshegaihi, Nesma M. Helal","doi":"10.1007/s11105-024-01479-w","DOIUrl":"https://doi.org/10.1007/s11105-024-01479-w","url":null,"abstract":"<p>Drought stress is a major limiting factor for rice production globally. The current climatic changes have further increased the incidence and duration of droughts worldwide. On the other hand, seaweed extracts have been extensively studied as biostimulants that improve plant growth, nutrition, quality, yield, and stress tolerance of rice (<i>Oryza sativa</i>). Different types of seaweeds have been employed for biostimulant preparation, of which algae is most commonly used. Of particular interest were <i>Galaxaura oblongata</i> and <i>Turbenaria ornate</i> seaweeds, as biostimulants that inoculate with soil in rice plants growing under drought conditions to boost the resilience of rice to drought. The incorporation of drought-stressed-rice soil with two investigated seaweeds led to improved growth, almost regulated levels of photosynthetic pigments, compatible solutes, MDA, hydrogen peroxide, and phenolic compounds as well as flavonoids, along with notably upregulated expression of <i>PIP1;4</i>, <i>PIP2;7</i>, <i>NCDE5</i>, and <i>OsMyb-R1</i> genes of the rice cultivars Giza 177 and Giza 179, and this enhanced the water status of rice cultivars grown under drought conditions. Furthermore, this study suggested that amending soil with investigated seaweeds enhanced nutrition and osmolyte production, ameliorated MDA, and upregulated investigated drought-responsive genes as well as promoted the growth along with antioxidant reply thoroughly contribute to improving rice plant tolerance to drought stress.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141769565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Visible LEDs as Technology to Delay Postharvest Senescence of Vegetables: A Review","authors":"Alejandra Bárcena, Gustavo Martínez, Lorenza Costa","doi":"10.1007/s11105-024-01480-3","DOIUrl":"https://doi.org/10.1007/s11105-024-01480-3","url":null,"abstract":"<p>In the last decade, a lot of research works and reviews have evaluated the effect of LED treatments during the postharvest storage of green vegetables. The curious thing is that, for the same vegetable, some works have shown that LED treatments improve postharvest life, while other researches have shown the opposite. Knowledge of the effects of light on the postharvest metabolism of each vegetable is needed to understand such contradictory results and to develop appropriate applications of postharvest LED technology to improve product quality. In this review, the role of visible light in the regulation of the postharvest senescence of green vegetables will be discussed. The information obtained so far suggests that low-intensity visible light, either continuous or pulsed, can maintain the quality of green tissues after harvest by delaying the onset of senescence and/or by improving their nutritional quality. The results also showed that the effect of LED technology depends on the duration of treatment, the intensity and the quality of the light used, and the type of vegetable irradiated. Studies have shown that both, white and red light, are effective to delay senescence, whereas blue light primarily enhances the antioxidant system with little or no effect in delaying senescence. To date, the published evidence strongly suggests that phytochromes (photoreceptors activated by red light) and phytochrome-interacting factors (PIFs) are involved in the delay of postharvest senescence symptoms.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141745838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Functional Characterization of nst1, nst2, and nst3 in Arabidopsis thaliana Uncovers Previously Unknown Functions in Diverse Developmental Pathways Beyond Secondary Wall Formation","authors":"Shobha Yadav, Komal Jalan, Sandip Das","doi":"10.1007/s11105-024-01474-1","DOIUrl":"https://doi.org/10.1007/s11105-024-01474-1","url":null,"abstract":"<p>The regulation of secondary cell wall formation in <i>Arabidopsis thaliana</i> has been extensively studied with <i>NST1</i>, <i>NST2</i>, and <i>NST3</i> playing key roles in secondary cell wall development in stem, anther, and silique. However, their broader impact on plant growth and development is less understood. This study investigates the phenotypes of T-DNA insertional mutants of <i>NST1</i> (<i>nst1-1</i>), <i>NST2</i>, <i>NST3</i> (<i>nst3-1</i>), and the double mutant <i>nst1-1nst3-1</i> revealing their previously unknown functions in traits crucial for plant fitness. Phylogenetic analysis of the NAC gene family, based on chromosome locations, suggests that local and segmental duplication has expanded the family. <i>NST1</i>, <i>NST2</i>, and <i>NST3</i> are phylogenetically close, within the same sub-clade, yet located on separate chromosomes, indicating a complex evolutionary history with functional redundancy and diversification. Loss-of-function mutants of <i>NST1</i>, <i>NST2</i>, and <i>NST3</i> (i.e., <i>nst1-1</i>, <i>nst2</i>, <i>nst3-1</i>, and <i>nst1-1nst3-1</i>) displayed changes in root and hypocotyl length, rosette leaf size and area, stem diameter, vascular bundle structure, stamen characteristics, and silique. The <i>nst3-1</i> single mutant exhibits the most pronounced defective phenotypes, emphasizing the pivotal role of <i>NST3</i> in governing various developmental processes. Furthermore, the compounded effects observed in the <i>nst1-1nst3-1</i> double mutant underscore the intricate interplay between these genes and their collective impact on <i>Arabidopsis</i> growth. Our findings indicate that the function of <i>NST1</i>, <i>NST2</i>, and <i>NST3</i> genes extends beyond their well-established roles in secondary wall regulation to significantly influence multiple aspects of plant growth and development, enhancing our understanding of their regulatory network in <i>Arabidopsis.</i></p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141548888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Cowpea (Vigna unguiculata) Mutant Lines for Dissecting Resilience to Drought Through Physiological and Molecular Crosstalk Analysis","authors":"Sara Diallo, François Abaye Badiane, Issa Diédhiou, Made Diouf, Mariama Ngom, Diaga Diouf","doi":"10.1007/s11105-024-01473-2","DOIUrl":"https://doi.org/10.1007/s11105-024-01473-2","url":null,"abstract":"<p>Cowpea (<i>Vigna unguiculata</i>) is a legume crop widely grown in tropical and subtropical regions for human consumption and animal feeding. Despite this importance, drought stress is a major constraint on cowpea production, affecting plant growth, seed quality, and yield. Thus, this study aimed to understand the mechanisms controlling drought tolerance in cowpea. To achieve this goal, two cowpea varieties (Ndout violet pods and Bambey 21) and one mutant line (Me51M4-39M9) were mutagenized using gamma rays. At M6, the seeds of the most productive and vigorous plants were identified, leading to the selection of 33 genotypes. They were sown and grown in the field to develop M7; then, the plants were subjected to drought stress for 38 days at an average daily temperature of 34.5 °C. The analyses focused on physiological parameters such as chlorophyll a and b and total contents and relative water content. In addition, malondialdehyde and proline contents; catalase, ascorbate peroxidase, and superoxide dismutase activities; and the expression of the <i>VunP5CS</i> and <i>VubZip09</i> genes were quantified. A wide range of variability of relative water content was observed among the mutant lines subjected to drought stress. Chlorophyll a and b and total contents varied under drought but increased in the stay-green genotype (4), while proline content increased significantly in some genotypes such as line 10 but decreased in the other mutant lines under drought. The amount of malondialdehyde decreased in some mutant lines and increased in others under drought stress in comparison to the unstressed control. The activities of catalase and ascorbate peroxidase increased under drought stress in mutant lines 4 (stay green) and 15 (early flowering). Based on the RT-qPCR analysis, proline and the <i>bZIP</i> transcription factor genes were highly expressed under drought in tolerant genotypes 4 and 15. This study revealed that cowpea responds to drought stress through complex processes involving multiple physiological, biochemical, and transcriptomic changes that need to be explored in depth to enhance our understanding of the genetic basis controlling drought tolerance.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141509896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Genetic Diversity in Gmelina arborea Roxb. Across Different Regions of Gujarat, India: The First Report","authors":"Almutazbellah K. Naji, Pooja G. Trivedi, Kush V. Prajapati, Maulika B. Panchal, Gaurang M. Sindhav","doi":"10.1007/s11105-024-01470-5","DOIUrl":"https://doi.org/10.1007/s11105-024-01470-5","url":null,"abstract":"<p><i>Gmelina arborea (G. arborea)</i>, a plant of great medicinal as well as socioeconomic importance renowned for its traditional utilization, lacks prior genetic diversity studies specifically in Gujarat, India. Therefore, the present study aimed to delve into the genetic variations within <i>G. arborea</i> across six distinct regions (Kutch, Sabarkantha, Ahmedabad, Rajkot, Panchmahal, and Valsad) in Gujarat. This investigation employed Geographical Information System (GIS) for spatial data evaluation, as well as DNA barcoding technique to corroborate the plant’s authenticity. Leveraging 15 different Inter Simple Sequence Repeats (ISSR) markers facilitated the evaluation of genetic diversity present amongst selected regions. Based on Jaccard’s dissimilarity co-efficient, a cluster analysis has been rendered by applying the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and Unweighted Neighbour Joining (UNJ) methods. The present study portrayed a significant level of polymorphism of 88% among <i>G. arborea</i> from different regions. The assessment of Polymorphic Information Content (PIC) resulted in a value of 0.42, indicating the presence of compelling genetic variation within these regions. The dendrogram illustrated the genetic relatedness and diversity, with a cophenetic correlation coefficient of 0.9818 (UPGMA) and 0.9962 (UNJ). The accession from the Ahmedabad region stands out as the most diverse, whereas the plant from Panchmahal represents the most primitive genome of all. Notably, this research marks the inaugural and comprehensive genetic assessment of <i>G. arborea</i> from Gujarat, India. These findings provide a valuable foundation for further research and conservation strategies for <i>G. arborea</i> and other medicinal plants.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141509895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a Stable Drought-Tolerant High-Yielding Line for Chickpea Crop Improvement","authors":"Santosh Kumar Gupta, Vikas Dwivedi, Nandakumar Surendra Kute, Philip Francis, Swarup Kumar Parida, Debasis Chattopadhyay","doi":"10.1007/s11105-024-01471-4","DOIUrl":"https://doi.org/10.1007/s11105-024-01471-4","url":null,"abstract":"<p>Chickpea (<i>Cicer arietinum</i> L.) is grown in marginal land with low input and is, therefore, drought-prone. In order to develop a drought-tolerant line, a bi-parental recombinant inbred line (RIL) mapping population was generated by inter-crossing between two varieties JGK3 (ICCV 95334) and Himchana1 (ICCX-810800) having contrasting root traits. Ninety-two genetically diverse RILs of F₈ generation were selected based on their total root length to root dry weight ratio (RL/DW). The leaf relative water content of these RILs under low soil moisture did not show any strong correlation with the RL/DW. Twenty RILs having high RL/DW were evaluated for seed yield in a field under rainfed condition without any supplementary irrigation. The best performing RIL, which performed better than the check varieties, was reevaluated for a further year under rainfed condition. The genotypic constitution of this superior low soil moisture tolerant individual RIL was determined by constructing its recombination map using genome-wide SNPs obtained through genotyping-by-sequencing. The RIL possesses the superior alleles of the genomic QTL region known to govern drought tolerance in chickpea. The phenotypic and genotypic characterization of RILs in our study identified a chickpea pre-breeding line that can be used as a genetic donor for developing drought-tolerant high-yielding chickpea varieties and our results provide an evidence that total root length to root dry weight ratio can be used as a quantitative trait for assessing drought tolerance.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141509898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: New Loci Relating to Rice Tiller Angle Traits Discovery Through Genome-Wide Association Analysis","authors":"Jubin Shi, Jinxin Gui, Xinyang Luo, Yuntao Yan, Xi He, Haiqing Zhang, Jiwai He","doi":"10.1007/s11105-024-01472-3","DOIUrl":"https://doi.org/10.1007/s11105-024-01472-3","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141368713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Overview of LEA Genes and Their Importance in Combating Abiotic Stress in Rice","authors":"Sarmistha Mohanty, P. Hembram","doi":"10.1007/s11105-024-01468-z","DOIUrl":"https://doi.org/10.1007/s11105-024-01468-z","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141112451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bulked Segregant RNA Sequencing (BSR-Seq) Combined with SNP Genotyping Towards Mapping and Characterization of a Purple Blotch Resistance Gene in Onion (Allium cepa L.)","authors":"Jayashree Sahoo, Rukmini Mishra, Raj Kumar Joshi","doi":"10.1007/s11105-024-01466-1","DOIUrl":"https://doi.org/10.1007/s11105-024-01466-1","url":null,"abstract":"<p>The pathogenic fungus, <i>Alternaria porri</i> (Ellis) Cifferi, that causes purple blotch (PB) disease, is a major constraint to production of onion and allied crops worldwide. In the present study, bulk segregant RNA sequencing (BSR-Seq) was used to analyze onion cultivar Arka Kalyan (resistant parent), Agrifound Rose (susceptible parent), and two sets of their bulks (20 homozygous resistant and 20 susceptible) from F₆ RIL population to identify a potential region for resistance to PB. Transcript profiling resulted in 278.08 million clean reads from 8 libraries. Comparative expression analysis revealed 755 differentially expressed genes (DEGs) including 492 upregulated and 263 downregulated sequences. Bulk frequency ratio (BFR) was estimated between resistant and susceptible bulk, and 2963 common SNPs with BFR &gt; 6 were detected on 1439 transcripts. Euclidean distance association analysis identified a 7.3 Mb resistance specific candidate region in the long arm of chromosome 6. Using RNA-Seq, 23 DEGs were reported in the candidate region in chromosome 6, including ACCL_20794 (Chr6: 187,639,724–187,643,297), a disease-resistant protein of the CC-NBS-LRR class, whose expression was elevated in the resistant pools following PB treatment. The ACCL_20794 gene was cloned and based on the sequences from the two parents, a single amino acid mutation—histidine (H) to serine (S) was detected in the resistance genotype Arka Kalyan. Quantitative reverse transcription (qRT)-PCR further demonstrated significantly differential expression of ACCL_20794 in the two parents as well as the RIL bulks. This indicates that ACCL_20794 might be the candidate resistance gene <i>ApR1</i> and is implicated in the PB resistance response.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141059490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}