Plant Molecular Biology Reporter最新文献

{"title":"Rice Transcriptomics Reveal the Genetic Determinants of An In Planta Photorespiratory Bypass: a Novel Way to Increase Biomass in C3 Plants","authors":"Parimalan Rangan, Dhammaprakash P. Wankhede, Rajkumar Subramani, Viswanathan Chinnusamy, Pooja Pathania, Arti Bartwal, Surendra K. Malik, Mirza Jaynul Baig, Anil Rai, Kuldeep Singh","doi":"10.1007/s11105-024-01469-y","DOIUrl":"https://doi.org/10.1007/s11105-024-01469-y","url":null,"abstract":"<p>Developing C₄ rice is one of the global research challenges for yield improvement. In the optimal environment, the key difference between C₃ and C₄ plants with reference to biomass accumulation is photorespiration. Photorespiration is important for a plant’s survival. In spite of the high energy cost and carbon loss, diversion of a significant part of carbon from photorespiration to enrich CO₂ concentration (preventing carbon loss) was opted for. Installation of photorespiratory bypasses was reported to improve biomass and yield in C₃ plants. The contribution of non-foliar photosynthesis to yield improvement was well documented. However, its underlying genetic differences, when compared to foliar photosynthesis, are a research gap. In three rice genotypes (APO, BAM4234, and CROSSA), we compared the expression levels (for genes associated with photosynthesis and photorespiration) between the photosynthetic non-foliar (3–5-day old developing grains and peduncle) and foliar (flag leaf) organs to understand their differential expression pattern using an RNA-seq approach. Significant downregulation of the genes of photorespiration was observed in non-foliar photosynthetic tissue (3–5 dpa old developing grains) when compared to the flag leaves. Simultaneously, our study also revealed significant upregulation of the chloroplastic pyruvate dehydrogenase (<i>cpPDC</i>, <i>BGIOSGA015796</i>) gene in developing grains, when compared to the flag leaf, in all three genotypes. The occurrence of an <i>in planta</i> photorespiratory bypass in the photosynthetic tissues of the developing grains in rice is proposed. Enhanced expression levels for the <i>cpPdc</i> gene in the foliar tissues will potentially install a photorespiratory bypass for enhanced biomass accumulation and thereby yield.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141059476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RL99 Was Essential for High Yield Formation by Regulating Leaf Morphogenesis and Root Development in Rice","authors":"Shenghai Ye, Menglin Liu, R. Zhai, Jing Ye, Mingming Wu, Guofu Zhu, Xiaoming Zhang","doi":"10.1007/s11105-024-01458-1","DOIUrl":"https://doi.org/10.1007/s11105-024-01458-1","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140977675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression Profiling of the 56 R2R3-MYB Family Genes in Response to Cold, Drought, and Salt Stress in Blue Honeysuckle (Lonicera caerulea L.)","authors":"Chun Xiang Bian, Haoyu Wang, Weihua Li, Jing Chen, Bingbing Ren, Dong Qin, Jiale Liu, Sitong Zang, Jiachen Li, Ke Ma, Junwei Huo, Huixin Gang","doi":"10.1007/s11105-024-01455-4","DOIUrl":"https://doi.org/10.1007/s11105-024-01455-4","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140972414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marker-Assisted Gene Pyramiding for Powdery Mildew Resistance in Thai Mungbean Variety SUT1 by Backcross Breeding","authors":"Pitchakon Papan, Witsarut Chueakhunthod, Apinya Khairum, Ketwalee Siwapitakpong, Chadapon Chaiyapan, Sukanya Inthaisong, Wanploy Jinagool, Akkawat Tharapreuksapong, Arada Masari, Chanwit Kaewkasi, Sumana Ngampongsai, Teerayoot Girdthai, Piyada Alisha Tantasawat","doi":"10.1007/s11105-024-01445-6","DOIUrl":"https://doi.org/10.1007/s11105-024-01445-6","url":null,"abstract":"<p>In Thailand, all Thai-certified varieties of mungbean [<i>Vigna radiata</i> (L.) R. Wilczek] preferentially grown by farmers are susceptible to powdery mildew (PM) disease. Marker-assisted gene pyramiding (MAGP) for improving resistant varieties is a sustainable strategy to overcome its outbreak in mungbean fields. In this study, two PM resistance genes from donor parent A2 were pyramided into the Thai-certified variety, namely Suranaree University of Technology 1 (SUT1) using marker-assisted backcrossing (MABC). Three polymorphic marker loci linked to PM resistance genes and three marker sets with 72 polymorphic loci were subsequently used for foreground and background selection, respectively. As a consequence, three pyramided BC₄ lines B1, B2, and D5, carrying all foreground marker loci in homozygosity, were obtained. Their recurrent parent genome (RPG) recovery was 100.0%, 100.0%, and 98.8% for background marker Sets A, B, and C, respectively. In field conditions, it was found that a low level of PM severity was exhibited in the pyramided BC line D5, which also had most of the agronomic traits similar or superior to SUT1. Moreover, the yield performance of this line was higher than SUT1, by up to 8.3–12.2%, during PM outbreaks or in the absence of disease. Thus, this pyramided BC line can be further used to develop a new resistant variety for farmers in the future.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140938082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosomal Positions of 5S and 45S rDNA in Some Iranian Fritillaria (Liliaceae) Species","authors":"Neda Seifoori, Ghader Mirzaghaderi, Shahla Hosseini","doi":"10.1007/s11105-024-01467-0","DOIUrl":"https://doi.org/10.1007/s11105-024-01467-0","url":null,"abstract":"<p>The present investigation systematically examined the distribution of 5S and 45S rDNA sites in six distinct <i>Fritillaria</i> species, all characterized as diploid with 2<i>n</i> = 2<i>x</i> = 24 chromosomes. <i>Fritillaria assyriaca</i> ecotypes displayed variable numbers of B chromosomes (Bs), ranging from one to four, while <i>F. zagrica</i> exhibited two additional B chromosomes. Terminal or sub-terminal chromosomal regions harbored one to two pairs of 5S rDNA sites. Regarding 45S rDNA sites, <i>F. raddeana</i> featured a singular pair, <i>F. assyriaca</i>, <i>F. zagrica</i>, and <i>F. persica</i> exhibited three pairs, <i>F. avromanica</i> presented four pairs, and <i>F. chlorantha</i> displayed eight pairs, predominantly situated distally, except for <i>F. raddeana</i> and <i>F. chlorantha</i>, which demonstrated interstitial and sub-terminal locations, respectively. Chromosome and karyotype indices facilitated the identification of, <i>F. avromanica</i> and <i>F. chlorantha</i> as species with the most symmetrical and asymmetrical chromosomes, respectively. Cluster analysis of the karyotype similarity matrix revealed incongruities between the observed number and distribution of rDNA sites and the established taxonomic classifications, particularly notable in <i>F. chlorantha</i> within the <i>Fritillaria</i> subgenus. The results provide significant insights into the genetic diversity and karyotype characteristics of <i>Fritillaria</i>, challenging conventional taxonomic frameworks. The observed variations in the numbers and locations of rDNA sites underscore the necessity for a nuanced understanding of genetic relationships within the <i>Fritillaria</i> subgenus.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140938257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Mapping of Quantitative Trait Loci for the Growth Habit in Peanuts Using Bulked Segregant Analysis","authors":"Qia Ning, Li Li, Hailong Liu, Xiaoshu Chen, Yongchao Lv, Xiaoping Sun, Yue Zhao, Meijun Li, Huayuan Gao, Yu Han, Ridan Sun, Lianxi Zhang","doi":"10.1007/s11105-024-01460-7","DOIUrl":"https://doi.org/10.1007/s11105-024-01460-7","url":null,"abstract":"<p>Lateral branch angle (LBA) is an important agronomic trait that represents the plant’s growth habit in peanuts (<i>Arachis hypogaea</i> L.), and obtaining the ideotype is the goal of breeders. Although numerous studies have been conducted in the past few decades on both spreading (prostrate) and erect growth habits, the underlying mechanism governing the LBA remains elusive. In this study, bulked segregant analysis coupled with next-generation sequencing (BSA‐seq) was employed to map major quantitative trait loci (QTL) controlling LBA. Spreading (S) and erect (E) bulks from progenies of a recombinant inbred line (RIL) population (Xuhua 13 × F458) were sequenced. Through Δ(SNP‐index) analysis, we identified eight regions on chromosome B05 among 152,650,001–159,955,000 bp and one region on chromosome B09 corresponding to the genomic interval of 154,908,001 to 154,918,000 bp. The regions on chromosome B05 were further narrowed down using single nucleotide polymorphism (SNP) markers, and the gene associated with peanut LBA was mapped around the marker 15‐156 (156,921,591 bp) containing one annotated gene named <i>Arahy.XAW92V</i>, which may play a role in regulating cell growth. The results presented herein not only demonstrate the efficacy of BSA‐seq as a rapid strategy for mapping quantitative traits, such as LBA, but also provide compelling evidence supporting <i>Arahy.XAW92V</i> as a promising candidate gene associated with LBA in peanuts.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140882298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Complete Chloroplast Genome of Inula britannica: Unraveling the Phylogeny, Evolution, and Taxonomic Significance of the Genus Inula","authors":"Lichai Yuan, Yang Ni, Jingling Li, Heyu Yang, Jingwen Yue, Xinyi Zhang, Chang Zhang, Haimei Chen, Chang Liu","doi":"10.1007/s11105-024-01453-6","DOIUrl":"https://doi.org/10.1007/s11105-024-01453-6","url":null,"abstract":"<p><i>Inula britannica</i> is a significant botanical species of plant in the Asteraceae family. The dried capitulum of this plant has been utilized as a traditional Chinese herbal remedy, exhibiting therapeutic properties in treating edema, alleviating nausea, and preventing vomiting. The formal designation for <i>Inula britannica</i> has been assigned to <i>Pentanema britannicum</i>. The whole chloroplast genome of <i>I. britannica</i> was constructed de novo in this study. The genomic characteristics were assessed and subsequently compared to those of five <i>Inula</i> species. <i>I. britannica</i>’s chloroplast genome had a length of 150,774 base pairs. The observed structure had a characteristic quadripartite arrangement, comprising a substantial single-copy section, a smaller single-copy region, and a pair of inverted repeat regions, measuring 82,451 bp, 18,423 bp, and 24,950 bp in length, respectively. The genome has been shown to have 109 distinct genes, comprising 79 protein-coding genes, four rRNA genes, and 26 tRNA genes. The chloroplast genome of <i>I. britannica</i> was found to have 49 lengthy repetitive sequences and 69 simple sequence repeats. The findings of the phylogenetic analysis revealed a significant correlation among <i>I. britannica</i>, <i>I. janopinca</i>, and <i>I. linariifolia</i>, followed by <i>I. hupehensis</i>. Our proposal suggests that “<i>Inula britannica</i>” is a more appropriate label for this particular species than “<i>Pentanema britannicum</i>.” Combining the K2p, ecorPrimers, and DNA polymorphic analysis results, eight regions can be distinguished from four <i>Inula</i> species after PCR and sangger sequencing. The reconstruction of ancestral character states revealed a series of changes from long spurs to alternative petal forms in the <i>Inula</i> species. The investigation of divergence time revealed that <i>Inula</i> underwent diversification during the late Pliocene period, specifically around 3.245 Mya. These findings will aid in establishing its evolutionary connections and taxonomy classification.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140882310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cutting-Edge of Genotyping by Sequencing (GBS) for Improving Drought and Salinity Stress Tolerance in Watermelon (Citrullus lanatus L.): A Review","authors":"Fildaus Nyirahabimana, İlknur Solmaz","doi":"10.1007/s11105-024-01465-2","DOIUrl":"https://doi.org/10.1007/s11105-024-01465-2","url":null,"abstract":"<p>Genotyping by sequencing (GBS) is a very helpful approach and one of the most useful techniques for examining and analysing the genetic variation of various lines and varieties. GBS technology was used to identify single nucleotide polymorphisms (SNPs) and assess genetic variation in several watermelon accessions. GBS application in watermelon breeding programs has recently become a popular technique among many breeders. Watermelon (<i>Citrullus lanatus</i> L<i>.</i>) is a warm-season crop that is widely cultivated for its delicious fruit. And it is one of the most economically significant crops in the world. However, watermelon cultivation is frequently hampered by abiotic stressors such as drought and salinity. Recently, there has been a growing body of research on the mechanisms that allow watermelon to tolerate these stresses and improve crop yield. Generally, cucurbits are beneficial to human health, they provide necessary minerals, fibre, and nutrient components. Therefore, this review demonstrates the cutting edge of using GBS technology to identify genetic design of several features in watermelon to improve abiotic stresses (drought and saline). The application of the GBS technique has provided a distinct advantage in watermelon breeding studies. Based on GBS approach, many new candidate genes in watermelon lines control a variety of traits including saline and drought tolerance, fruit rind color, disease tolerance, nutrient components, size, and fruit shape were discovered. Modern breeding techniques are being used to develop economically viable vegetable crops that will meet customer preferences and needs. Further research is needed to enhance watermelon production.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140882256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TuMV Infection Alters the Regulation of miR168/AGO1 and miR403/AGO2 Systems in Arabidopsis","authors":"Carlos Augusto Manacorda, Sabrina Tasselli, María Rosa Marano, Sebastian Asurmendi","doi":"10.1007/s11105-024-01461-6","DOIUrl":"https://doi.org/10.1007/s11105-024-01461-6","url":null,"abstract":"<p>Plant argonaute (AGO) proteins—chiefly AGO1 and 2—restrict viral infections. AGO1/2 also participate in developmental processes and are tightly regulated by microRNAs. Researchers have conducted extensive studies on the regulatory loop involving miR168/AGO1 in viral infections, though comparatively less attention has been given to the miR403/AGO2 system. Here, we simultaneously studied both regulatory systems in <i>Arabidopsis</i> plants infected with turnip mosaic virus (TuMV). TuMV simultaneously altered both miR168 and miR403 precursors as well as their mature forms at medium to late stages of infection. While TuMV decreased miRNA precursor molecules, it induced the overaccumulation of mature miRNA forms, without evidence of concomitant transcriptional alteration. The AGO1 protein remained at basal levels, whereas the AGO2 protein overaccumulated. The application of exogenous salicylic acid (SA) in healthy plants resulted in elevated AGO2 mRNA levels. Conversely, this hormone did not induce any significant changes in either AGO1 mRNA levels or those of miRs 168 and 403. This response is coherent with previous results, which showed enhanced levels of SA under TuMV infection and the partially differential sensitivity that AGO proteins have against this defense hormone. Our results also highlight the key role of AGO2 in leaves as an antiviral molecule and demonstrate the different responsiveness of the AGO1/miR168 and AGO2/miR403 systems regarding TuMV infection and SA response. Taken together, the results presented here are in line with previous reports studying abiotic and biotic impacts on microRNA biogenesis and AGO-dependent antiviral defense and further expand the knowledge of the miR403/AGO2 regulatory system.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140837717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Identification of CYP450 Enzymes and New Insights into Their Response to Diverse Abiotic Stresses in Brassica napus","authors":"Haili Song, Yingpeng Hua, Ting Zhou, Caipeng Yue, JinYong Huang, Yingna Feng","doi":"10.1007/s11105-024-01448-3","DOIUrl":"https://doi.org/10.1007/s11105-024-01448-3","url":null,"abstract":"<p>Cytochrome P450 (CYP450) proteins are a large group of monooxygenase that play important roles in the biosynthesis of secondary metabolites and degradation of xenobiotics. However, the responses of CYP450 family to abiotic stresses have not been characterized in <i>Brassica napus</i> (<i>B. napus</i>). In this study, we identified a total of 384 CYP450 genes in Darmor-<i>bzh</i>, the rapeseed culture whose genome was wildly used as a reference for gene clone. The structure and localization analyses showed that <i>BnaCYP450</i> genes have integrated heme-binding motif, contain 1–10 exons, unevenly distributed across all the 19 chromosomes, and mainly localized on chloroplast. Cis-regulation element analysis suggested that <i>BnaCYP450</i> genes were transcriptionally regulated by hormone and multiple stress response signals. Transcript expression analyses identified 108, 85, 96, and 86 <i>BnaCYP450s</i> differentially expressed genes (DEGs) in response to salt stress, potassium deficiency, nitrogen stress, and cadmium toxicity, respectively. Gene ontology (GO) enrichment analysis indicated that these <i>BnaCYP450</i> DEGs mainly enriched in molecular function of ion binding and oxidoreductase activity and the biological process of secondary product metabolism. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that they mainly involved in the pathway of isoflavonoid biosynthesis. Differential expression of <i>BnaCYP450s</i> to multiple abiotic stresses revealed the functional diversity of BnaCYP450 family in <i>B. napus</i>. This study gave a basic understanding of <i>CYP450</i> genes in <i>B. napus</i> and provides multiple core <i>BnaCYP450</i> genetic resources for improving plant resistance to multiple abiotic stresses.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140808940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}