Plant Molecular Biology Reporter最新文献

{"title":"Genetic Insights into Stress Resilience via Enhancing Salinity Adaptation in Barley","authors":"Samar G. Thabet, Amr Elkelish, Khairiah Mubarak Alwutayed, Ahmad M. Alqudah","doi":"10.1007/s11105-024-01450-9","DOIUrl":"https://doi.org/10.1007/s11105-024-01450-9","url":null,"abstract":"<p>Salinity stress is one of the major abiotic stresses that significantly impacts plant growth and performance. Therefore, this study investigated the role of foliar-applied ascorbic acid (AsA) at a concentration of 2 mM as a mitigative approach to salt stress in barley during its vegetative stage. The research aimed to elucidate the genetic responses induced by AsA that potentially confer enhanced tolerance to salinity. A controlled environment experiment was conducted, wherein barley plants were subjected to salt stress and treated with a foliar application of AsA. Natural phenotypic variation showed an increment for all agronomical traits after the foliar application of AsA compared to saline environment (control). Highly significant markers were detected by using a genome-wide association study (GWAS) that are associated with all of the studied traits, underscoring the protective role of AsA under salt stress. Furthermore, the study identified several novel stress-responsive genes influenced by AsA treatment, pointing to complex genetic networks underlying salt stress mitigation. For instance, the gene <i>HORVU.MOREX.r3.2HG0199440</i> at position (622,219,977–622,220,459 bp) on 2H, which is annotated as V-type ATP synthase beta chain, helps to maintain cellular redox balance during stress conditions. This research paves the way for future work on the practical applications of AsA in agriculture, especially in breeding programs aimed at enhancing salt stress tolerance in barley and similar cereals.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"37 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140602285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Overexpression of Solanum nigrum Osmotin (SnOLP) Boosts Drought Response Pathways in Soybean","authors":"","doi":"10.1007/s11105-024-01452-7","DOIUrl":"https://doi.org/10.1007/s11105-024-01452-7","url":null,"abstract":"<h3>Abstract</h3> <p>Environmental stresses are responsible for limiting soybean yield. To mitigate the impacts generated by water deficit, molecular biology tools are being used to develop genetically modified plants. Previous studies showed that two independent events (B1 and B3) of soybean transgenic plants expressing a <em>Solanum nigrum</em> osmotin (SnOLP) had an increment in drought tolerance. The present study aims to investigate the modulated pathways that results in the drought tolerance promoted by osmotin overexpression in soybean. Transgenic and non-transgenic (NT) plants in the vegetative stage were submitted to water deficit by irrigation suppression for seven days. Control plants were kept irrigated. Physiological variables were monitored and confirmed that the transgenic plants present better performance when compared to the NT plants. The total RNA extracted from leaves was sequenced and data was normalized by DESeq2. A total of 2044 and 1505 differentially expressed genes (DEGs) were identified in B1 and B3 events, respectively. Regarding the B1 event, 769 genes were upregulated and 1275 downregulated. For B3, 541 genes were upregulated and 964 genes were downregulated. Excluding common differentially expressed genes (DEGs) between transgenic and non-transgenic (NT) plants yielded 395 upregulated and 234 downregulated genes, which were shared by B1 and B3 events. The metabolic pathways and gene ontology categories identified are known to be involved in plant responses to drought. Hormonal, photosynthetic, carbohydrate and amino acid metabolism, reactive oxygen species, and post-translational modifications pathways were significantly modulated in transgenic plants. Altogether, the results suggest that osmotin promotes tolerance through an increment in the plant responses elicited by drought. </p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"2016 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140583909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marker-Assisted Simultaneous and Stepwise Pyramiding of Broad-Spectrum Bacterial Leaf Blight Resistance Genes, Xa33 and Xa38, into Salt-Tolerant Rice Variety “CO43”","authors":"Gizachew Haile Gidamo, Kuumaravadivel Nachimuthu","doi":"10.1007/s11105-024-01451-8","DOIUrl":"https://doi.org/10.1007/s11105-024-01451-8","url":null,"abstract":"<p>Bacterial leaf blight (BLB) disease is one of the most important diseases of rice in India and worldwide. The management of this disease becomes extremely challenging due to the presence of <i>Xoo</i> races with varying level of virulence and diversity. The development of BLB-resistant rice lines with the help of marker-assisted selection has been proven as one of the most effective strategies to sustain rice cultivation. This study demonstrates marker-assisted introgression and pyramiding of two broad-spectrum BLB genes (<i>Xa33</i> and <i>Xa38</i>) into the background of “CO43,” a popular fine white-grained rice cultivar, tolerant to saline coastal areas in southern India. Two separate marker-assisted backcrossing schemes were used, and at each stage of backcrossing, foreground, recombinant, and background selection were carried out. Backcross-derived lines possessing either <i>Xa33</i> or <i>Xa38</i> along with maximum genome recovery of “CO43” were identified at BC₂F₁ generation, intercrossed with each other to pyramid the genes, and selfed to identify homozygous lines for the pyramided genes. The performance of the improved “CO43” lines, such as lines # 2_7_78, 2_7_102, 6_9_65, and 6–8-76 exhibited enhanced broad-spectrum resistance to BLB (lesion length, LL 0.0 ± 0.0 to 0.9 ± 0.1 cm). Furthermore, the improved “CO43” lines maintained the agro-morphological features of the recurrent parent along with enhanced BLB resistance. These lines with effective and durable resistance would contribute to sustainable rice production in coastal areas of southern India.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"100 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140602116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity and Population Structure of Lablab (Lablab purpureus L. Sweet) Accessions from Ethiopia Using SSR Markers","authors":"Solomon Tamiru Workneh, Tileye Feyissa, Zemede Asfaw, Tesfaye Disasa","doi":"10.1007/s11105-024-01447-4","DOIUrl":"https://doi.org/10.1007/s11105-024-01447-4","url":null,"abstract":"<p>Lablab is a regionally important multipurpose legume crop used for human consumption, animal feed, and soil conservation. Despite these qualities, the potential value of this crop has not been fully utilized, and very little research attention has been given to it. The main objective of the study was molecular genetic diversity analysis of Lablab collections using 15 SSR markers. The molecular genetic diversity study of 91 Lablab collections revealed a total of 225 alleles with an average of 14.80 alleles per locus. All markers across the entire population were found to be highly polymorphic and informative with PIC values ranging from 0.78 to 0.92 with a mean value of 0.85. The average expected heterozygosity and gene diversity were 0.75 and 0.86 respectively, indicating a high level of diversity. Analysis of molecular variance showed that 94% of the total genetic variation was attributed to within populations, while only 6% was attributed to among populations. The fixation index value (0.061) recorded indicates the presence of moderate population differentiation as a result of high gene flow (Nm = 3.820) among populations. Due to high gene flow, Cluster, PCoA, and structure analysis did not exactly categorize the populations into genetic groups corresponding to their geographic origin. The observed relatively higher genetic diversity in Konso and West Wellega populations among the eight populations indicates that these areas could be considered hot-spots for genetic diversity and possible germplasm evaluation. Generally, genetic diversity obtained from this study provides inputs for Lablab conservation and improvement in Ethiopia.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"19 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140301842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SSR Marker–Based Genetic Diversity and Relationship Analyses of Stephania tetrandra S. Moore","authors":"Mengli Wu, Yanyuan Bai, Liwei Huang, Zhipeng Dong, Yaoyan Li, Guoyue Yan, Yangjiao Xie","doi":"10.1007/s11105-024-01449-2","DOIUrl":"https://doi.org/10.1007/s11105-024-01449-2","url":null,"abstract":"<p><i>Stephania tetrandra</i> S. Moore (family: Menispermaceae), a dioecious herbaceous vine and the only species in the subgenus <i>Botryodiscia</i> of the genus <i>Stephania</i> of the family Menispermaceae, is mainly distributed in hilly areas south of the Huaihe River in China and found in many provinces of China, showing a high genetic diversity. This paper aimed to study genetic diversity of and genetic relationship among individuals of <i>S. tetrandra</i> within China to provide a basis for evaluation, exploitation, and utilization of <i>S. tetrandra</i> by using simple sequence repeat (SSR) molecular markers. Our results show that effective products were amplified from the 26 screened SSR gene loci, a total of 183 alleles amplified (2–16 alleles amplified by each pair of primers). Among the 26 loci, 16 had a <i>PIC</i> value higher than 0.5, indicating a high level of polymorphism. For most of the loci, the number of effective alleles was lower than that of the observed alleles, and the observed heterozygosity was lower than the expected heterozygosity. The genetic differentiation coefficient (0.021–0.547) was lower than 0.05 (low level of genetic differentiation) for 7 loci and higher than 0.25 (very high level of genetic differentiation) for 2 loci, and had a value representing a medium level of genetic differentiation for the remaining 17 loci. The intra-population inbreeding coefficient had a positive value for 21 loci, suggesting the presence of inbreeding and homozygous excess. The gene flow value was bigger than 1, indicating that genetic drift and natural selection played an unimportant role in population genetic differentiation of <i>S. tetrandra</i>. Based on discriminant analysis of principal components and Bayesian Information Criterion, <i>K</i>-means clustering was performed on 620 samples. These samples were divided into 9 genetic clusters, whose similarity coefficients and genetic distances were 0.755–0.918 and 0.067–0.280, respectively, indicating that these clusters were highly similar and short-distanced. The Bayesian clustering analysis was implemented in the STRUCTURE software to analyze the genetic structure of <i>S. tetrandra</i> and it was found that the 620 samples could be clustered into 5 ancestor groups; the 9 clusters and 40 natural populations inherited genes from the 5 groups to varying degrees, but the proportion of genes inherited from the 5 groups by each cluster and natural population differed. <i>S. tetrandra</i> was characterized by the presence of population structure and pronounced genetic subdivision, which, together with the presence of gene flow, may indicate a relatively stable recent state of these populations.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"7 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140196841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Functional Analysis of Na+/H+ Antiporter Gene (LcNHX1) from Leymus chinensis","authors":"Chuanbo Sun, Chuang Zhang, Yuejia Yin, Ying Wang, Shujing Mu, Qing Liu, Ziyu Wang, Yang Liu, Chunxiao Zhang, Xiangguo Liu, Yan Zhang, Jia Guo","doi":"10.1007/s11105-024-01446-5","DOIUrl":"https://doi.org/10.1007/s11105-024-01446-5","url":null,"abstract":"<p>Saline-alkali stress has adverse effects on plant growth. Some plant Na⁺/H⁺ antiporters were reported to be important in salt tolerance. However, it needs to be better understood that Na⁺/H⁺ antiporters are involved in plant salt-alkali (NaHCO₃/Na₂CO₃) tolerance. In this study, a Na⁺/H⁺ antiporter gene <i>LcNHX1</i> (China patent No.200810050629.1) has been cloned from <i>Leymus chinensis.</i> The <i>LcNHX1</i> CDS contains 1614 bp that encodes 537 amino acids<i>.</i> Amino acid and nucleotide sequence similarity, protein topology modelling, conserved functional domains in the protein sequence, and subcellular localization classified <i>LcNHX1</i> as a vacuolar <i>NHX1</i> homolog. Transcription analysis by quantitative RT-PCR indicated that upregulated expression of <i>LcNHX1</i> could be induced by NaCl, NaHCO₃, NaCl + NaHCO₃, and PGE in <i>L. chinensis</i> seedlings. The expression of <i>LcNHX1</i> partially complements the salt-sensitive phenotypes of a <i>Δnhx1</i> yeast strain. In addition, <i>LcNHX1</i> overexpressing enhanced the tolerance to NaHCO₃ stress in the transgenic Arabidopsis. Taken together, these results indicated that <i>LcNHX1</i> is a potential candidate gene for enhancing plant saline-alkali tolerance.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"121 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140196838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Variation and Population Structure of Rhododendron anthopogon Along an Altitudinal Gradient: A Case Study from Himalaya","authors":"","doi":"10.1007/s11105-024-01438-5","DOIUrl":"https://doi.org/10.1007/s11105-024-01438-5","url":null,"abstract":"<h3>Abstract</h3> <p>High-altitude regions like the Himalaya, where various slopes are present on a very small spatial scale, are one of the best natural laboratories for investigating how genetic diversity and population structure are distributed across altitudinal gradients. In this study, we investigate the magnitude of genetic diversity and population structure of <em>Rhododendron anthopogon</em> D.Don along an altitudinal gradient in the Kashmir Himalaya. We used the start codon targeted (SCoT) marker to evaluate the genetic diversity and differentiation between different populations across five sites along an altitudinal gradient ranging from 3200 to 4000 m (amsl). Our results demonstrate that the magnitude of genetic diversity differs among the populations of <em>R. anthopogon</em> along the altitudinal gradient. We observed a decreasing trend in genetic diversity with increasing altitude from site-1 to site-3 (3200 to 3600 m), and an increasing trend from site-4 to site-5 (3601 to 4000 m). Furthermore, we also observed the highest genetic diversity at upper altitude (site-5) and the lowest at mid-altitude (site-3). Our results reveal a high genetic differentiation (<em>G</em>_st = 0.7349) and a low gene flow (<em>N</em>_<em>m</em> = 0.1804) among the populations. Analysis of molecular variance (AMOVA) reveals 71% variation among the populations and 29% variation within the populations. We also reveal positive associations between geographic and genetic distances, indicating isolation by distance effect across all altitudes. STRUCTURE and cluster analysis divided all the populations of <em>R. anthopogon</em> into five clusters according to their geographical location. Overall, the study adds to our understanding that altitude is the major driver of genetic variation and differentiation between populations of <em>R. anthopogon</em> which may help increase its range under ongoing projected climate change. Our study also reveals that populations at higher altitudes are not genetically isolated, suggesting that they might be an essential source for migration in the era of climate change.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"24 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140069939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Proteomics Study Revealing the Impact of Growing Region and Cultivar on Protein Expression in the Leaves of Nicotiana tabacum Plants","authors":"Yongxu Hu, Min Chen, Gang Li, Da Wu, Xiangmin Zhang","doi":"10.1007/s11105-024-01444-7","DOIUrl":"https://doi.org/10.1007/s11105-024-01444-7","url":null,"abstract":"<p>The physical and chemical properties of tobacco (<i>Nicotiana tabacum</i> L.) plants are sensitive to changes in genetics and the environment. However, few studies have investigated the effect of both cultivar and regional factors on tobacco quality at the proteomic level. Here, a TMT-based quantitative proteomics method was used to investigate proteome profiling of different tobacco leaves under various geographical locations. In total, 8587 proteins were detected, among which 300 differentially abundant proteins (DAPs) were identified. Proteins associated with carbohydrate metabolism and amino acid metabolism were more abundant in tobacco plants from Yunnan. In contrast, proteins involved in the response to heat were more abundant in tobacco plants from Henan. We found that proteins related to carbon metabolism and defense signaling played an important role in the characteristics of different cultivars within the same region. In this work, we identified key proteins and pathways involved in the response of <i>Nicotiana tabacum</i> to environmental change and explored the proteomic differences among cultivars. Our results provide a better understanding of the effect of environment and cultivar on the tobacco leaf proteome, which will be helpful for elucidating the molecular mechanisms of the formation of tobacco characteristic quality.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"52 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted Metabolomics Reveals Altered Pathways in Phytoplasma-Infected Sesame Plants","authors":"","doi":"10.1007/s11105-024-01440-x","DOIUrl":"https://doi.org/10.1007/s11105-024-01440-x","url":null,"abstract":"<h3>Abstract</h3> <p><em>Sesamum indicum</em> is an important oilseed crop rich in nutrients and antioxidants, cultivated worldwide owing to its economic value. Sesame fields are often challenged due to a disease caused by <em>Candidatus</em> Phytoplasma, a cell wall-lacking bacteria. Phytoplasma infection results in alteration in the plant’s physiological and metabolic responses. As metabolites are the final products that aid in a plant’s adaptation to several biotic and abiotic stresses, it is necessary to understand these metabolic changes. However, the changes at the metabolite level after Phytoplasma infection in sesame are poorly understood. To investigate the response of sesame plants to Phytoplasma infection, we analyzed the metabolic changes using liquid chromatography-tandem mass spectrometry (LC–MS/MS/MS). Data analysis led to the detection of 162 metabolites, among which 82 were upregulated, while 62 exhibited downregulating trends in the Phytoplasma-infected tissue. The upregulated metabolites include flavonoids, phenolics, lignans, glucosinolates, terpenoids, phytohormones, and some sugars, like trehalose. Phytoplasma infection also resulted in significant chlorophyll breakdown, suggesting impaired chloroplast physiology. We have also validated this LC–MS/MS data by biochemical analysis and analyzing the expression of a few genes behind the biosynthesis of the concerned metabolites using qRT-PCR analysis. The results indicate that plant metabolic networks undergo reprogramming upon Phytoplasma infection. Understanding this altered metabolic pathway may aid in developing a control measure for this disease.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"233 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140035332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought Priming May Enhance the Tolerance of Cotton Seedlings to Subsequent Drought Stress","authors":"Yousheng Tian, Zhijun Wang, Panpan Ma, Zengqiang Zhao, Zongming Xie","doi":"10.1007/s11105-024-01437-6","DOIUrl":"https://doi.org/10.1007/s11105-024-01437-6","url":null,"abstract":"<p>Enhancing water use efficiency is a key strategy to improve drought resistance in cotton. Although drought priming has been recognized for enhancing plant tolerance to drought, its impact on upland cotton remains uncertain. In a pot trial with Xinluzao19, we studied the influence of soil drought and drought priming on seedlings. Primed plants maintained similar height to non-primed ones but showed significant differences in hydrogen peroxide, malondialdehyde, and enzyme activities, indicating improved reactive oxygen species (ROS) homeostasis. Transcriptome analysis revealed 1441 upregulated genes and 12,024 downregulated genes in RD6D compared to D6D. GO enrichment and KEGG metabolic pathway analysis also revealed that numerous stress-related genes and defense pathways were significantly enriched. Some genes linked to ascorbate peroxidase and superoxide dismutase displayed similar expression profiles with enzymatic activities. These insights contribute to enhancing cotton and overall crop productivity, particularly under water-deficit conditions.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"5 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140006845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}