Plant Molecular Biology Reporter最新文献_第5页

Analysis of Expression, Mutation, and Alternative Splice Variants of Candidate Genes, MLO2 and MLO6A, Involved in Powdery Mildew Susceptibility in Mulberry (Morus spp.) 参与桑树（Morus spp.）白粉病易感性的候选基因 MLO2 和 MLO6A 的表达、突变和替代剪接变体分析

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-04-27 DOI: 10.1007/s11105-024-01457-2

Manudeep Rao, A. Ramesha, Himanshu Dubey, Naleen, Pawan Shukla, Kangayam M. Ponnuvel, V. Sivaprasad, K. Suresh

{"title":"Analysis of Expression, Mutation, and Alternative Splice Variants of Candidate Genes, MLO2 and MLO6A, Involved in Powdery Mildew Susceptibility in Mulberry (Morus spp.)","authors":"Manudeep Rao, A. Ramesha, Himanshu Dubey, Naleen, Pawan Shukla, Kangayam M. Ponnuvel, V. Sivaprasad, K. Suresh","doi":"10.1007/s11105-024-01457-2","DOIUrl":"https://doi.org/10.1007/s11105-024-01457-2","url":null,"abstract":"Mulberry is a fast-growing perennial crop commercially exploited as the sole source of food for the mulberry silkworm, Bombyx mori, for the production of silk. Powdery mildew disease is a major foliar disease that greatly affects the sericulture industry. Understanding the genetic basis of powdery mildew disease resistance and its utilization for developing resistant varieties is the primary approach to mulberry crop improvement. Both natural and induced mutations impairing the function of the Mildew resistance Locus O (MLO) gene leading to powdery mildew resistance have been identified in different crop plants. However, more studies are required focusing on MLO gene-based resistance in natural germplasm. In this study, previously identified candidate genes, MLO2 and MLO6A, involved in powdery mildew susceptibility in mulberry were analyzed for gene expression, mutation, and alternative splice variants. In some tolerant accessions, MLO2 and MLO6A show either reduced or no elevated expression under infected conditions. Publicly available whole genome re-sequenced data analysis from mulberry accessions identified single amino acid substitutions in the MLO2 and MLO6A genes. Further, alternative splice variants of intron retention and exon skipping resulted in premature stop codon leading to the production of truncated MLO2 protein which were detected in tolerant mulberry accessions. This study will pave for better understanding of powdery mildew disease resistance and for breeding program.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Csy4-Processing TRV-Based CRISPR/Cas9 Genome Editing System in Nicotiana benthamiana 在烟草属植物中开发基于 Csy4 处理 TRV 的 CRISPR/Cas9 基因组编辑系统

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-04-25 DOI: 10.1007/s11105-024-01459-0

Yanjie Luo, Xiaodong Tang, Cuihong Yu, Yang Qiu, Julia S. Nowak, Qing Shi Lu, Lining Tian, Ren Na

引用次数: 0

New Loci Relating to Rice Tiller Angle Traits Discovery Through Genome-Wide Association Analysis 通过全基因组关联分析发现与水稻分蘖角度性状有关的新基因座

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-04-25 DOI: 10.1007/s11105-024-01464-3

Jubin Shi, Jinxin Gui, Xinyang Luo, Yuntao Yan, Xi He, Haiqing Zhang, Jiwai He

引用次数: 0

Proximity Labelling Confirms the Involvement of Papain-Like Cysteine Proteases and Chaperones in Cyclotide Biosynthesis 近似标记证实木瓜蛋白酶和伴侣蛋白参与了环苷酸的生物合成

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-04-23 DOI: 10.1007/s11105-024-01462-5

Meng-Han Lee, Mark A. Jackson, Fabian B. H. Rehm, Deborah S. Barkauskas, W. L. Ho, K. Yap, D. Craik, E. Gilding

引用次数: 0

Genome-Wide Characterization of Nuclear Microsatellite Markers for Centranthus trinervis (= Valeriana trinervis) and Cross-Amplification in Five Congeneric Taxa Centranthus trinervis（= Valeriana trinervis）核微卫星标记的全基因组特征及在五个同源类群中的交叉扩增

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-04-23 DOI: 10.1007/s11105-024-01454-5

E. di Iorio, D. De Luca, B. Menale, Carole Piazza, G. Bacchetta, O. de Castro

引用次数: 0

Dissection of Physiological Traits for Terminal Heat Stress Tolerance in Indian Mustard (Brassica juncea L.) Using Microsatellite Markers 使用微卫星标记分析印度芥菜（Brassica juncea L.）耐受终端热胁迫的生理特征

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-04-23 DOI: 10.1007/s11105-024-01456-3

R. Choudhary, Ram Avtar, Pawan Kumar, Manjeet Singh, Mahavir Bishnoi, Neeraj Kumar, R. Punia

引用次数: 0

Revealing the Novel Genetic Resources for Blast Resistance in Diverse Rice Landraces of North-Eastern Hills of Himalayas 揭示喜马拉雅山东北丘陵地区不同水稻品种抗瘟性的新型遗传资源

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-04-20 DOI: 10.1007/s11105-024-01463-4

Riya Raj, Amit Kumar, Balakrishnan C, Philanim W. S, Letngam Touthang, S. P. Das, Mayank Rai, Veerendra Kumar Verma, Vinay Kumar Mishra

{"title":"Revealing the Novel Genetic Resources for Blast Resistance in Diverse Rice Landraces of North-Eastern Hills of Himalayas","authors":"Riya Raj, Amit Kumar, Balakrishnan C, Philanim W. S, Letngam Touthang, S. P. Das, Mayank Rai, Veerendra Kumar Verma, Vinay Kumar Mishra","doi":"10.1007/s11105-024-01463-4","DOIUrl":"https://doi.org/10.1007/s11105-024-01463-4","url":null,"abstract":"Rice blast disease is one of the most disastrous diseases causing significant losses to the crop. In the humid weather conditions of north-eastern Himalayan region, the situation is highly devastating as the climate is very favorable to the fungus Magnaporthe oryzae. Development of resistant rice varieties is the most effective, economical, and environment-friendly way to control this disease. The study aimed to identify novel sources of resistant donor using agro-morphological and gene-based markers for their utilization in development of blast-resistant varieties with high yield potential. Phenotypic evaluation has classified the hundred landraces into resistant (13), moderately resistant (43), moderately susceptible (24), and susceptible (20). Fifty-nine genotypes were found to carry genes responsible for blast resistance, either singly or in combination. The genotype MN-62 was found to have a blast score of zero in field screening. The genetic frequencies of the major blast resistance genes ranged from 28 to 97%. The cluster analysis grouped the landraces into three major groups. Additionally, the association between blast genes was computed with blast score using a general linear model (GLM) and mixed linear model (MLM), revealing that the markers AP4007, AP56595, C1454, and RM208 were found to be associated with the trait. The landraces with six blast R-genes like Nungshang Phou, Ratkhara, Anandhi, RCPL 1–44, Ching Phou Khong Nombi, MN-31, Rona Yang, and RCPL-128 were also superior for agro-morphological traits. Similarly, the landraces MN-62, Tara Bali, Rato Bhan Joha, MN-47, RCPL 1–411, and CAU-R1 were promising regarding yield and blast resistance. The resistant lines with various combinations of blast resistance genes can be utilized for introgression of genes into the commercial varieties for durable resistance using marker-assisted backcrossing.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140626119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Insights into Stress Resilience via Enhancing Salinity Adaptation in Barley 通过增强大麦对盐度的适应性提高抗逆性的遗传学见解

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-04-15 DOI: 10.1007/s11105-024-01450-9

Samar G. Thabet, Amr Elkelish, Khairiah Mubarak Alwutayed, Ahmad M. Alqudah

{"title":"Genetic Insights into Stress Resilience via Enhancing Salinity Adaptation in Barley","authors":"Samar G. Thabet, Amr Elkelish, Khairiah Mubarak Alwutayed, Ahmad M. Alqudah","doi":"10.1007/s11105-024-01450-9","DOIUrl":"https://doi.org/10.1007/s11105-024-01450-9","url":null,"abstract":"Salinity stress is one of the major abiotic stresses that significantly impacts plant growth and performance. Therefore, this study investigated the role of foliar-applied ascorbic acid (AsA) at a concentration of 2 mM as a mitigative approach to salt stress in barley during its vegetative stage. The research aimed to elucidate the genetic responses induced by AsA that potentially confer enhanced tolerance to salinity. A controlled environment experiment was conducted, wherein barley plants were subjected to salt stress and treated with a foliar application of AsA. Natural phenotypic variation showed an increment for all agronomical traits after the foliar application of AsA compared to saline environment (control). Highly significant markers were detected by using a genome-wide association study (GWAS) that are associated with all of the studied traits, underscoring the protective role of AsA under salt stress. Furthermore, the study identified several novel stress-responsive genes influenced by AsA treatment, pointing to complex genetic networks underlying salt stress mitigation. For instance, the gene HORVU.MOREX.r3.2HG0199440 at position (622,219,977–622,220,459 bp) on 2H, which is annotated as V-type ATP synthase beta chain, helps to maintain cellular redox balance during stress conditions. This research paves the way for future work on the practical applications of AsA in agriculture, especially in breeding programs aimed at enhancing salt stress tolerance in barley and similar cereals.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140602285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Overexpression of Solanum nigrum Osmotin (SnOLP) Boosts Drought Response Pathways in Soybean 黑茄科植物 Osmotin (SnOLP) 的过表达可促进大豆的干旱响应途径

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-04-06 DOI: 10.1007/s11105-024-01452-7

{"title":"The Overexpression of Solanum nigrum Osmotin (SnOLP) Boosts Drought Response Pathways in Soybean","authors":"","doi":"10.1007/s11105-024-01452-7","DOIUrl":"https://doi.org/10.1007/s11105-024-01452-7","url":null,"abstract":"<h3>Abstract</h3> Environmental stresses are responsible for limiting soybean yield. To mitigate the impacts generated by water deficit, molecular biology tools are being used to develop genetically modified plants. Previous studies showed that two independent events (B1 and B3) of soybean transgenic plants expressing a Solanum nigrum osmotin (SnOLP) had an increment in drought tolerance. The present study aims to investigate the modulated pathways that results in the drought tolerance promoted by osmotin overexpression in soybean. Transgenic and non-transgenic (NT) plants in the vegetative stage were submitted to water deficit by irrigation suppression for seven days. Control plants were kept irrigated. Physiological variables were monitored and confirmed that the transgenic plants present better performance when compared to the NT plants. The total RNA extracted from leaves was sequenced and data was normalized by DESeq2. A total of 2044 and 1505 differentially expressed genes (DEGs) were identified in B1 and B3 events, respectively. Regarding the B1 event, 769 genes were upregulated and 1275 downregulated. For B3, 541 genes were upregulated and 964 genes were downregulated. Excluding common differentially expressed genes (DEGs) between transgenic and non-transgenic (NT) plants yielded 395 upregulated and 234 downregulated genes, which were shared by B1 and B3 events. The metabolic pathways and gene ontology categories identified are known to be involved in plant responses to drought. Hormonal, photosynthetic, carbohydrate and amino acid metabolism, reactive oxygen species, and post-translational modifications pathways were significantly modulated in transgenic plants. Altogether, the results suggest that osmotin promotes tolerance through an increment in the plant responses elicited by drought. ","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140583909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marker-Assisted Simultaneous and Stepwise Pyramiding of Broad-Spectrum Bacterial Leaf Blight Resistance Genes, Xa33 and Xa38, into Salt-Tolerant Rice Variety “CO43” 标记辅助将抗广谱细菌性叶枯病基因 Xa33 和 Xa38 同时并逐步分级到耐盐水稻品种 "CO43 "中

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-04-04 DOI: 10.1007/s11105-024-01451-8

Gizachew Haile Gidamo, Kuumaravadivel Nachimuthu

{"title":"Marker-Assisted Simultaneous and Stepwise Pyramiding of Broad-Spectrum Bacterial Leaf Blight Resistance Genes, Xa33 and Xa38, into Salt-Tolerant Rice Variety “CO43”","authors":"Gizachew Haile Gidamo, Kuumaravadivel Nachimuthu","doi":"10.1007/s11105-024-01451-8","DOIUrl":"https://doi.org/10.1007/s11105-024-01451-8","url":null,"abstract":"Bacterial leaf blight (BLB) disease is one of the most important diseases of rice in India and worldwide. The management of this disease becomes extremely challenging due to the presence of Xoo races with varying level of virulence and diversity. The development of BLB-resistant rice lines with the help of marker-assisted selection has been proven as one of the most effective strategies to sustain rice cultivation. This study demonstrates marker-assisted introgression and pyramiding of two broad-spectrum BLB genes (Xa33 and Xa38) into the background of “CO43,” a popular fine white-grained rice cultivar, tolerant to saline coastal areas in southern India. Two separate marker-assisted backcrossing schemes were used, and at each stage of backcrossing, foreground, recombinant, and background selection were carried out. Backcross-derived lines possessing either Xa33 or Xa38 along with maximum genome recovery of “CO43” were identified at BC2F1 generation, intercrossed with each other to pyramid the genes, and selfed to identify homozygous lines for the pyramided genes. The performance of the improved “CO43” lines, such as lines # 2_7_78, 2_7_102, 6_9_65, and 6–8-76 exhibited enhanced broad-spectrum resistance to BLB (lesion length, LL 0.0 ± 0.0 to 0.9 ± 0.1 cm). Furthermore, the improved “CO43” lines maintained the agro-morphological features of the recurrent parent along with enhanced BLB resistance. These lines with effective and durable resistance would contribute to sustainable rice production in coastal areas of southern India.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140602116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0