Plant Molecular Biology Reporter最新文献_第6页

Genome-Wide Association Studies for Identification of Novel QTLs Related to Agronomic Traits in Horsegram (Macrotyloma uniflorum) 进行全基因组关联研究以鉴定与马齿苋（Macrotyloma uniflorum）农艺性状相关的新 QTLs

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-02-27 DOI: 10.1007/s11105-023-01430-5

Ankita Sharma, Rakesh Kumar Chahota

{"title":"Genome-Wide Association Studies for Identification of Novel QTLs Related to Agronomic Traits in Horsegram (Macrotyloma uniflorum)","authors":"Ankita Sharma, Rakesh Kumar Chahota","doi":"10.1007/s11105-023-01430-5","DOIUrl":"https://doi.org/10.1007/s11105-023-01430-5","url":null,"abstract":"Horsegram (Macrotyloma uniflorum) is an important legume crop well adapted to harsh environments and has significant nutritional value. It is also well recognized due to its nutritional value, health benefits, drought tolerance, and ability to improve soil fertility. It is a versatile crop used in various dishes and is a valuable addition to any diet. However, in-depth molecular analysis must be improved to understand the molecular architecture regulating the essential traits. To achieve this, the study utilized a genome-wide association mapping approach using 88 horsegram accessions genotyped with 55,656 single nucleotide polymorphisms (SNPs). A total of 54,271 SNPs were mapped to the reference genome. Phenotypic data were collected for four agronomic and yield-related traits: plant height, days to maturity, days to flowering, and number of branches per plant. These traits played a significant role in plant growth, development, protection, and crop yield. The study identified 79 significant SNP-trait associations for the traits under study. The identified SNPs were in 8 chromosomes. The study also identified several novel genomic regions associated with the traits considered, providing new insights into the genetic basis of these essential traits in horsegram. Overall, the study highlights the potential of genome-wide association mapping for identifying genomic regions associated with important agronomic and yield-related traits in horsegram. This could facilitate the development of improved horsegram varieties through marker-assisted breeding programmes and overcoming the morphological selection of desirable genotypes in the background of narrow genetic base.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"51 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140007076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into Blossom End-Rot Disorder in Tomato (Solanum lycopersicum) 番茄（Solanum lycopersicum）花末腐烂病的深入研究

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-02-24 DOI: 10.1007/s11105-024-01442-9

Khageswar Sethi, Surjeet Singh Dhaka, Vinay Kumar Bari

{"title":"Insights into Blossom End-Rot Disorder in Tomato (Solanum lycopersicum)","authors":"Khageswar Sethi, Surjeet Singh Dhaka, Vinay Kumar Bari","doi":"10.1007/s11105-024-01442-9","DOIUrl":"https://doi.org/10.1007/s11105-024-01442-9","url":null,"abstract":"Blossom end rot (BER) is mainly a calcium (Ca2+) deficiency-related physiological disorder of fruits that affects various crop production worldwide including tomato (Solanum lycopersicum). The visible symptoms of BER include cell wall disintegration, cell plasmolysis, and water-soaked signs at the distal end of the fruits. During fruit development increase in cell expansion and a decrease in the transport of Ca2+ to the distal part of the fruits, can lead to the development of BER. It is hypothesized that insufficient Ca2+ is available for essential apoplastic and cytoplasmic functions during the cell expansion phase of fruits when the cellular Ca2+ demand exceeds the Ca2+ supply. Therefore, abnormal intracellular Ca2+ content or signals, cause weakening of cell walls, and a loss of cellular integrity, potentially leading to cell death and the outward manifestations of BER. The occurrence of BER in tomatoes is also influenced by environmental factors that affect the cellular growth of the fruits. These factors such as drought, high salinity, high temperature, insufficient xylem tissue development, phytohormones, and oxidative stress can influence the development of BER. The availability of a high-quality reference genome and whole genome sequencing allowed us to identify selected loci that can cause BER, facilitating genetic dissection and a deeper comprehension of the molecular mechanisms underlying this disorder. This review summarized the various factors and genes involved in BER development and management strategies.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"4 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139953586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Mechanisms of Male Sterility in Maize 玉米雄性不育的分子机制

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-02-23 DOI: 10.1007/s11105-024-01441-w

Xiaoyang Chen, Yunfei Li, Huayue Sun, Xueli An, Jihua Tang

引用次数: 0

Phenotypic and Genetic Variation Studies in Finger Millet Genotypes to Blast Disease Caused by Pyricularia grisea 手指黍基因型对灰霉病的表型和遗传变异研究

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-02-23 DOI: 10.1007/s11105-024-01436-7

Swagata Thakur, Sanjay Kumar Jain, Vinay Kumar, Ganesan Prakash, Mallikarjuna Jeer, Pankaj Kaushal

{"title":"Phenotypic and Genetic Variation Studies in Finger Millet Genotypes to Blast Disease Caused by Pyricularia grisea","authors":"Swagata Thakur, Sanjay Kumar Jain, Vinay Kumar, Ganesan Prakash, Mallikarjuna Jeer, Pankaj Kaushal","doi":"10.1007/s11105-024-01436-7","DOIUrl":"https://doi.org/10.1007/s11105-024-01436-7","url":null,"abstract":"The most detrimental disease of finger millet (Eleusine coracana) is blast caused by Pyricularia grisea inflicting substantial yield losses. This study evaluated 100 finger millet genotypes from a core collection for disease response to leaf, neck and finger blast. Good phenotypic variability in the genotypes was recorded for blast reactions. None of the accessions was resistant to leaf blast; however, 12 and 27 accessions were resistant to neck and finger blast, respectively. A total of 28 accessions showed resistance to both neck and finger blast. A significant positive correlation was observed between neck and finger blast. Genotyping of 50 accessions differing in disease reactions to neck blast was performed using 30 SSR markers 17 of which proved to be polymorphic. A total of 51 alleles were detected with the mean value of 2.55 alleles per locus. The PIC values of the polymorphic SSR markers ranged from 0.03 to 0.98, and the dendrogram grouped these genotypes in 13 clusters. Cluster I and II comprised of resistant accessions, whereas cluster VIII formed the susceptible group. SSR markers UGEP 76 and UGEP 102 consistently produced bands in neck blast susceptible and highly susceptible germplasms, indicating their probable association with neck blast susceptibility gene(s). Our results showed that the core collection has appreciable diversity for blast reactions both phenotypically and genotypically.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"80 7 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139953580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigate the Genetic Relationships and Morphological Diversity of Some Afghan and Iranian Melon Cultivars for Breeding Purposes 为育种目的调查阿富汗和伊朗甜瓜栽培品种的遗传关系和形态多样性

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-02-20 DOI: 10.1007/s11105-024-01433-w

Moazam Haqmal, Maryam Haghighi, Mahdi Rahimmalek, Mahboobeh Hodaei

{"title":"Investigate the Genetic Relationships and Morphological Diversity of Some Afghan and Iranian Melon Cultivars for Breeding Purposes","authors":"Moazam Haqmal, Maryam Haghighi, Mahdi Rahimmalek, Mahboobeh Hodaei","doi":"10.1007/s11105-024-01433-w","DOIUrl":"https://doi.org/10.1007/s11105-024-01433-w","url":null,"abstract":"To investigate the genetic diversity of 40 melon and cantaloupe cultivars, which were collected from the central regions of Iran and southwestern Afghanistan, inter-simple sequence repeat (ISSR) primers and several morphological traits were used. The results demonstrate that 12 selected ISSR markers generated polymorphic bands with a distinct band pattern. There were a total of 150 bands created, with 95 bands exhibiting polymorphism (62.44% diversity). Based on shape and characteristics, cluster analysis and principal coordinate analysis (PCoA) classified the cultivars into five groups: winter and late ripening, medium ripening, sweet, and early melons, including cantaloupe and melon. In the majority of instances, PCoA was consistent with cluster analysis, whereas the molecular data did not perfectly match the morphological results. The results of the analysis classified the morphological characteristics of individuals into five groups: the first group comprised nine Iranian and Afghan melon genotypes with long, thin-skinned forms and intermediate medium ripening, and the second group comprised five Afghan melon genotypes with late ripening, winter ripe, and thick skin and flesh. The third group of 11 Iranian and Afghan melon cultivars was typically medium-sized, elliptical-shaped melons with thin skin. The fourth group consists of two Iranian melons with small seeds, small fruit, rapid maturation, and no flavor. The final group possessed dense and tender flesh, a spherical shape, and large seeds. In conclusion, morphological traits and ISSR analysis could be useful tools for classifying melon germplasm for future breeding applications. The morphological and molecular similarities between Afghan and Iranian cultivars suggest their origin.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"11 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139922164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome‑Wide Identification and Expression Analysis of SPX Domain-Containing Gene Subfamily in Response to Phosphorus-Solubilizing Bacteria in Apple (Malus domestica) 苹果（Malus domestica）中含 SPX 结构域基因亚家族对磷溶解细菌反应的全基因组鉴定和表达分析

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-02-17 DOI: 10.1007/s11105-024-01443-8

Mehmet Kural, Ali Kiyak, Selman Uluisik, Ersin Atay

{"title":"Genome‑Wide Identification and Expression Analysis of SPX Domain-Containing Gene Subfamily in Response to Phosphorus-Solubilizing Bacteria in Apple (Malus domestica)","authors":"Mehmet Kural, Ali Kiyak, Selman Uluisik, Ersin Atay","doi":"10.1007/s11105-024-01443-8","DOIUrl":"https://doi.org/10.1007/s11105-024-01443-8","url":null,"abstract":"Phosphate is one of the major elements that significantly affects fruit yield and quality. The aim of the study was to determine whether using phosphorus-solubilizing bacteria could produce high-quality apple nursery trees. Five different treatments were tested on a “Granny Smith” apple cultivar that was grafted onto an M.9 rootstock. These were 100% P, 50% P, 50% P + Bacillus megatarum (plant growth promoting rhizobacteria, PGPR), 0%P, and 0%P + PGPR. The study also identified the SPX gene family, which is essential for plant growth and development and responds to phosphorus (P) stress. A total of 72 SPX genes were identified in different plant species based on structural and phylogenetic analysis. The apple genome contains seven different SPX genes distributed on five of the 17 chromosomes. Gene structure and motif analysis showed that SPX genes show a relatively conserved exon/intron arrangement and motif composition in five different species: apple, strawberry, peach, apricot, and grape. Protein–protein network analysis showed that SPX proteins are closely related to proteins involved in P metabolism in apple. The digital expression profiles of MdSPX genes among 47 apple tissues were characterized to provide insight into their potential functions. RT-qPCR revealed that the expression level of all MdSPXs was significantly downregulated in 50% P + PGPR treatments, indicating that 50% P combined with PGPR is effectively taken up by the plant, saving it from Pi starvation. These results not only confirm the key role of MdSPXs in Pi homeostasis and the Pi signaling pathway but also clarify the importance of Pi-solubilizing bacteria in plant nutrition.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"25 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139764343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advancements in Nitrogen Use Efficiency in Crop Plants Achieved by Genomics and Targeted Genetic Engineering Approaches 通过基因组学和定向基因工程方法提高作物氮利用效率的最新进展

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-02-16 DOI: 10.1007/s11105-024-01439-4

{"title":"Recent Advancements in Nitrogen Use Efficiency in Crop Plants Achieved by Genomics and Targeted Genetic Engineering Approaches","authors":"","doi":"10.1007/s11105-024-01439-4","DOIUrl":"https://doi.org/10.1007/s11105-024-01439-4","url":null,"abstract":"<h3>Abstract</h3> Nitrogen plays a crucial role in plant metabolism, growth, and development of plants, and its deficiency leads to severe growth retardation and reduced grain yield. The efficient utilization of nitrogenous fertilizers is needed to enhance crop yield and also to fetch the food demand of the world population. The accumulated nitrogen in the ecosystem leads to severe environmental pollution and health hazards to inhabited animals. However, nitrogen inside plants is regulated by a set of nitrogen metabolism genes, promoters, and transcription factors. Further, the identification and characterization of nitrogen metabolism genes in crop plants is a prerequisite for developing tailored crop plants for increased nitrogen use efficiency (NUE), grain yield, biomass, and other economic traits. Moreover, NUE is a complex trait, and breeding crops for improving NUE is still in the infancy stage. Therefore, a targeted and holistic approach is required for enhanced nitrogen uptake and its utilization. The precise modulation of key genes of nitrogen metabolism, amino acid biosynthesis, and carbon metabolism could result in enhancement of NUE, and the engineered crop plants for NUE traits were reported to be superior in terms of NUE and also incurred higher grain yield, biomass, and improved agronomical parameters as that of cultivated crop cultivars. In this review, we described the basics of nitrogen metabolism, genomics, and recently targeted genetic engineering strategies employed in crop plants for improving NUE.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"4 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139764502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Approaches to Improve Legume Salt Stress Tolerance 提高豆科植物耐盐性的分子方法

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-02-12 DOI: 10.1007/s11105-024-01432-x

Ahmed El Moukhtari, Nadia Lamsaadi, Cécile Cabassa, Mohamed Farissi, Arnould Savouré

{"title":"Molecular Approaches to Improve Legume Salt Stress Tolerance","authors":"Ahmed El Moukhtari, Nadia Lamsaadi, Cécile Cabassa, Mohamed Farissi, Arnould Savouré","doi":"10.1007/s11105-024-01432-x","DOIUrl":"https://doi.org/10.1007/s11105-024-01432-x","url":null,"abstract":"The global challenge of crop loss due to salt stress became increasingly significant, especially in the context of meeting the rising demands of a growing world population. This review focuses on the impact of salt stress on leguminous plants throughout their entire growth stages. Additionally, it provides a comprehensive overview of the molecular strategies employed to enhance the performance of legumes in saline environments. In addressing this issue, the review critically assesses recent advancements in bolstering legume salt stress tolerance through genetic engineering. This approach is acknowledged for its efficiency compared to traditional breeding methods, facilitating the transfer of desired genes without introducing extraneous genetic material from the donor organism. The review also examines the critical role of preventing ionic toxicity in transgenic leguminous plants by expressing foreign Na+/H+ antiporter genes and transcription factors. Furthermore, the review emphasizes the positive outcomes observed when introducing or overexpressing genes related to compatible solutes in transgenic legumes. These genetic modifications have proven effective in enhancing the tolerance of legumes to salinity-induced osmotic stress. Another aspect explored in the review is the improving of salt stress-induced oxidative stress management in various transgenic legume species. This is achieved through the expression of both enzymatic and non-enzymatic genes. Finally, the review explores the manipulation of candidate genes to improve nodule performance under salt stress. By identifying and modifying specific genes, researchers can pave the way for leguminous plants to thrive in salt-affected environments.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"59 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139764424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Germin-Like Proteins (GLPs) in Biotic and Abiotic Stress Responses in Major Crops: A Review on Plant Defense Mechanisms and Stress Tolerance 类胚芽鞘蛋白 (GLP) 在主要作物的生物和非生物胁迫响应中的作用：植物防御机制与抗逆性综述

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-01-30 DOI: 10.1007/s11105-024-01434-9

Ganesan Govindan, Sandhiya K R, Vinoth Alphonse, Suji Somasundram

{"title":"Role of Germin-Like Proteins (GLPs) in Biotic and Abiotic Stress Responses in Major Crops: A Review on Plant Defense Mechanisms and Stress Tolerance","authors":"Ganesan Govindan, Sandhiya K R, Vinoth Alphonse, Suji Somasundram","doi":"10.1007/s11105-024-01434-9","DOIUrl":"https://doi.org/10.1007/s11105-024-01434-9","url":null,"abstract":"Germin-like proteins (GLPs) play crucial roles in disease resistance, stress tolerance, and plant defense responses in various crop species. This review explores the intricate role of GLP gene expression and its modulation by cis-acting regulatory elements (CAREs) under biotic and abiotic stress in major crops. In rice, OsGLP2-1 and OsGLP3-7 have been identified as positive regulators of disease resistance. Similarly, TaGLP genes in wheat and VvGLP3 in grapes have been associated with powdery mildew resistance. Additionally, ZmGLP1 in maize and StGLP5 in potato contribute to defense against Bipolaris maydis and salt stress, respectively. AhGLPs in peanuts respond to drought stress, while GmGLP10 in soybean demonstrates a response to Sclerotinia sclerotiorum infection. Research in cotton has unveiled GhABP19 and GhGLP2 as GLPs involved in plant defense responses to wilt disease. Analysis of GLP gene promoters has revealed the presence of stress-responsive CAREs that modulate gene expression under biotic and abiotic stresses. Transgenic overexpression of GLP genes in different plant species, such as potato, tobacco, and Arabidopsis, has resulted in enhanced resistance to fungal pathogens, oxidative stress, and abiotic stresses. CRISPR/Cas9 genome editing has provided insights into UV-B stress response mechanisms. Promising outcomes from transgenic studies and CRISPR genome editing present exciting opportunities to improve disease resistance and stress tolerance in crops. These findings significantly enhance our understanding of the critical roles played by GLPs in crop resilience, paving the way for the development of stress-resistant crops to ensure sustainable global food security.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"32 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139648240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression Analysis of Metacaspase (MC) Gene Family in Response to Ethylene Signal During Apple Fruit Ripening 苹果果实成熟过程中对乙烯信号响应的 Metacaspase (MC) 基因家族的表达分析

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-01-22 DOI: 10.1007/s11105-024-01435-8

Mingyu Sun, Jingyi Lv, Yingzhi Zhang, Liang Zhang, Jingxin Chen, Yonghong Ge, Jianrong Li

{"title":"Expression Analysis of Metacaspase (MC) Gene Family in Response to Ethylene Signal During Apple Fruit Ripening","authors":"Mingyu Sun, Jingyi Lv, Yingzhi Zhang, Liang Zhang, Jingxin Chen, Yonghong Ge, Jianrong Li","doi":"10.1007/s11105-024-01435-8","DOIUrl":"https://doi.org/10.1007/s11105-024-01435-8","url":null,"abstract":"To better understand the relationship between MdMCs and ethylene during apple fruit ripening. “Golden Delicious” apple fruit were treated with ethephon (0.2 mmol L−1) and ethylene action inhibitor 1-methylcyclopropene (1-MCP) (1 µL L−1) after harvest, and the ethylene-responsive transcription factor MdERF2 was transiently over-expressed in apple fruit at commercial maturity. Our results showed that climacteric peaks of respiration rate and ethylene production were advanced by ethephon treatment while delayed by application of 1-MCP. Ethephon treatment increased activity of MC, content of malondialdehyde (MDA), and relative electrical conductivity (REC) compared with controls during ripening, while 1-MCP treatment had the opposite effects. Expression of MdMC04, MdMC07, MdMC12, MdMC14, MdMC16, MdMC18, MdMC19, and MdMC20 was differentially induced by ethephon treatment, while their expression was generally downregulated by application of 1-MCP compared with controls during ripening. MdMC06 expression was induced at the late ripening stage by 1-MCP treatment but reduced by ethephon application during ripening compared to the control group. Transcript levels of MdMC01 and MdMC13 were promoted by both 1-MCP and ethephon treatments during ripening. In contrast, expression of MdMC17, MdMC05, MdMC02, MdMC21, MdMC10, and MdMC09 was differentially reduced after application of ethephon and 1-MCP during ripening compared with controls. Expression patterns of MdMCs varied in fruit transiently over-expressing MdERF2 during ripening. These results indicated that MdMCs expression was modulated by ethylene signal during apple ripening. Our findings would be useful for further unraveling the molecular mechanism of ethylene signaling in modulating MC genes during apple ripening.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"21 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139518289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0