Phenotypic and Genetic Variation Studies in Finger Millet Genotypes to Blast Disease Caused by Pyricularia grisea

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-02-23 DOI:10.1007/s11105-024-01436-7

Swagata Thakur, Sanjay Kumar Jain, Vinay Kumar, Ganesan Prakash, Mallikarjuna Jeer, Pankaj Kaushal

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Abstract

The most detrimental disease of finger millet (Eleusine coracana) is blast caused by Pyricularia grisea inflicting substantial yield losses. This study evaluated 100 finger millet genotypes from a core collection for disease response to leaf, neck and finger blast. Good phenotypic variability in the genotypes was recorded for blast reactions. None of the accessions was resistant to leaf blast; however, 12 and 27 accessions were resistant to neck and finger blast, respectively. A total of 28 accessions showed resistance to both neck and finger blast. A significant positive correlation was observed between neck and finger blast. Genotyping of 50 accessions differing in disease reactions to neck blast was performed using 30 SSR markers 17 of which proved to be polymorphic. A total of 51 alleles were detected with the mean value of 2.55 alleles per locus. The PIC values of the polymorphic SSR markers ranged from 0.03 to 0.98, and the dendrogram grouped these genotypes in 13 clusters. Cluster I and II comprised of resistant accessions, whereas cluster VIII formed the susceptible group. SSR markers UGEP 76 and UGEP 102 consistently produced bands in neck blast susceptible and highly susceptible germplasms, indicating their probable association with neck blast susceptibility gene(s). Our results showed that the core collection has appreciable diversity for blast reactions both phenotypically and genotypically.

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手指黍基因型对灰霉病的表型和遗传变异研究

指粟（Eleusine coracana）最有害的病害是由灰霉病（Pyricularia grisea）引起的穗瘟，会造成巨大的产量损失。本研究评估了 100 个核心收集的小米基因型对叶瘟、颈瘟和指瘟的病害反应。基因型对穗轴疫病反应的表型变异性很高。没有一个基因型对叶瘟有抗性，但分别有 12 个和 27 个基因型对颈瘟和指瘟有抗性。共有 28 个品种同时对颈瘟和指瘟表现出抗性。颈瘟和指瘟之间存在明显的正相关。使用 30 个 SSR 标记对 50 个对颈瘟的疾病反应不同的品种进行了基因分型，其中 17 个标记被证明具有多态性。共检测到 51 个等位基因，每个位点的等位基因平均值为 2.55 个。多态 SSR 标记的 PIC 值在 0.03 到 0.98 之间，树枝图将这些基因型分为 13 个群组。簇 I 和簇 II 是抗性品种，而簇 VIII 则是易感品种。SSR 标记 UGEP 76 和 UGEP 102 在颈瘟感病和高感病种质中持续产生条带，表明它们可能与颈瘟感病基因有关。我们的研究结果表明，核心收集的品种在表型和基因型上都具有明显的颈瘟反应多样性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.