Plant Molecular Biology Reporter最新文献

{"title":"Comprehensive Analysis of the R2R3-MYB Transcription Factor Gene Family in Salvia miltiorrhiza and the Regulatory Role of SmMYB50 in Salvianolic Acid Metabolism","authors":"Huiyan Fan, Jingzhi Zhou, Yuqi Ying, Kaijie Tang, Jiayi Han, Qiuying Wang, Guoyin Kai","doi":"10.1007/s11105-025-01647-6","DOIUrl":"https://doi.org/10.1007/s11105-025-01647-6","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147888915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Conservation of Ulmus wallichiana, Threatened Himalayan Species by Combining Population Genetics and Species Distribution Modeling","authors":"Amandeep Singh, Vikas Sharma, Aasim Majeed, Puneeta Pandey, Pankaj Bhardwaj","doi":"10.1007/s11105-025-01565-7","DOIUrl":"https://doi.org/10.1007/s11105-025-01565-7","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"43 3","pages":"1536-1550"},"PeriodicalIF":0.0,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147906785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Genetic Diversity: Advancing Wheat Resilience and Nutritional Fortification Through Introgression Against Climate Challenges","authors":"Taruna Bhagat, Tuhina Dey, Ashish Sheera, K Shruthi","doi":"10.1007/s11105-024-01525-7","DOIUrl":"https://doi.org/10.1007/s11105-024-01525-7","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"43 3","pages":"1086-1098"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147909867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockout of the Bread Wheat CER9/SUD1 Gene Using CRISPR/Cas Technology","authors":"Khalit Musin, Elena Mikhaylova, Aizilya Galimova, Elvina Baimukhametova, Evgenia Zaikina, Azat Kuluev, Zarina Ibragimova, Irina Rakhmatullina, Zoya Berezhneva, Bulat Kuluev","doi":"10.1007/s11105-024-01495-w","DOIUrl":"https://doi.org/10.1007/s11105-024-01495-w","url":null,"abstract":"<p>Mutations in the <i>CER9</i> gene of <i>Arabidopsis thaliana</i> L. contribute to the amplification of the cuticular wax and consequently mitigation of water loss, thereby fortifying drought resilience. Recently, genes homologous to <i>CER9</i>, termed <i>SUD1</i> genes, have been annotated in bread wheat (<i>Triticum aestivum</i> L.). However, no research has been done on these genes in <i>T. aestivum</i>. Hence, our study aimed to employ CRISPR/Cas technology to knock out the <i>CER9/SUD1</i> gene orthologs in bread wheat. For this, five guide RNAs were meticulously chosen and merged into a singular vector. Delivery of the CRISPR/Cas components was arranged through <i>Agrobacterium tumefaciens</i>, utilized for transforming immature embryos of two agricultural spring bread wheat varieties: Taya and Sigma. Among the 13 transgenic plants procured, four manifested positivity for the reporter gene <i>GFP</i> and <i>Cas9</i> gene. Notably, substantial deletions ranging from 284 to 398 bp within the <i>CER9/SUD1</i> gene were discerned in these plants. Additionally, two of the edited plants exhibited an absence of <i>CER9/SUD1</i> transcripts, while the other two displayed a noteworthy 5.4-fold reduction in <i>CER9/SUD1</i> gene expression compared to the wild type. Intriguingly, the genome-edited plants of the T₁ generation showcased enhanced growth parameters compared to the wild type under both standard and drought conditions.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"16 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142264820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QTL Mapping and Candidate Gene Analysis for Wax Trait of Stem and Leaf in Durum Wheat (Triticum turgidum L. ssp. durum (Desf.))","authors":"Yijing Cai, Yasir Muhammad, Yujie Zhou, Yuefen Cao, Junkang Rong, Xin Hu","doi":"10.1007/s11105-024-01497-8","DOIUrl":"https://doi.org/10.1007/s11105-024-01497-8","url":null,"abstract":"<p>The waxy cuticle on plant surfaces, comprising a cutin polymer matrix and complex very long chain fatty acids, figure in protective barrier against water loss and environmental damage. In this study, we employed a F₂ population (comprising of 237 individual plants) constructed by the crossing of HX128 (female parent without cuticular wax) with HX027 (male parent with significant cuticular wax). Two extreme phenotypic bulks were constructed according to the variation of wax trait in F₂ population, and bulked segregant RNA-seq (BSR-seq) was implemented to decipher the molecular underpinnings of waxiness trait in durum wheat. The QTL related to waxiness was mapped between 0.8 and 23.4 Mb on chromosome 2B. According to the differential sequence information of target interval between the parents, polymorphic primers were designed to screen F₂ population. The QTL of waxiness trait was further narrowed down between 6.8 and 10.1 Mb using the composite interval mapping tool. The LOD value was 129.53, with phenotypic variance explained (PVE) up to 44.65%. The annotation and expression analysis of the genes in the QTL region entailed selection of 15 potential candidate genes. Among which, eleven new genes with four previously reported genes were found that seem to be a gene cluster mediating wax synthesis at <i>W1</i> loci. This study provides important insights into fine mapping and cloning of genes involved in wax synthesis with improved efficacy.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"123 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142191239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Molecular Markers Associated with Genomic Regions Controlling Agronomic Traits in Bread Wheat Genotypes Under Different Moisture Conditions","authors":"Fatemeh Bavandpouri, Ezatollah Farshadfar, Kianoosh Cheghamirza, Mohsen Farshadfar, Mohammad Reza Bihamta, Amir Mohammad Mahdavi, Nadali Jelodar","doi":"10.1007/s11105-024-01494-x","DOIUrl":"https://doi.org/10.1007/s11105-024-01494-x","url":null,"abstract":"<p>The study of the association between polymorphism at the DNA level and the diversity of phenotypic traits is an essential tool in breeding programs. To identify informative microsatellite markers related to agronomic traits, this research including 25 bread wheat genotypes was carried out. The experiment was set up in a randomized complete block design with three replications in rainfed and irrigated conditions during two cropping seasons (2018–2020) in the cold Mediterranean climate of Iran. Variance analysis showed significant differences between genotypes for most of the traits. The 16 microsatellite primers out of 20 had considerable polymorphisms, and three markers, namely XCFD168-2D, XGWM350-7D, and XGWM136-1A, were introduced as the most significant markers for subsequent studies. Cluster analysis by the UPGMA method classified 25 wheat genotypes into four groups. Genotypes 1, 3, and 25 have the most significant genetic distance with genotypes 13, 7, and Pishgam. Association analysis by stepwise regression showed that in both years under rainfed conditions, the XGWM350 marker for 1000-grain weight, the XCFD5 marker for spike length, and the XGWM165 and XGWM70 markers for spike dry weight, and under irrigated conditions, the XGWM265 marker for grain yield exhibited significant associations. Also, the XGWM136 and XCFD5 were found to be common markers associated with agronomic traits for all the test environments. In addition, most of the markers were associated with 1000-grain weight, mitt penalty length, and spike grain weight in rainfed conditions and 1000-grain weight in irrigated conditions. After identifying molecular markers related to increased yield and drought tolerance, they can be used as selection criteria to accelerate wheat breeding programs. Also, these marker-trait associations can help wheat improvement programs through marker-assisted selection.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"40 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142191234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Analyses of Akebiae Fructus and Identification of Genes Related to Triterpenoid Saponin Biosynthesis","authors":"Wentao Fang, Chengcheng Qian, Rui Wu, Yatian Yang, Jinmei Ou","doi":"10.1007/s11105-024-01496-9","DOIUrl":"https://doi.org/10.1007/s11105-024-01496-9","url":null,"abstract":"<p>Akebiae Fructus (AF) is the dry and near-ripe fruit of <i>Akebia trifoliata</i> (Thunb.) Koidz. Triterpenoid saponins are important characteristic components of AF, but their molecular biosynthetic pathway has not yet been identified. In this study, DNBSEQ was used to sequence the transcriptome of the five stages of AF from May to September. A total of 96.87 Gb of data were assembled, including 115,430 unigenes with an average sequence length of 1445 bp. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses for metabolic pathway enrichment identified 434 unigenes involved in two metabolic pathways related to triterpenoid saponin biosynthesis, of which 255 encoded 19 key enzymes in the triterpenoid saponin biosynthetic pathway. Sequence analyses and homology modeling of the key enzyme, β-amyrin synthase (β-AS), which is implicated in triterpenoid saponin biosynthesis, showed that it has a conserved substrate-binding domain. Differentially expressed genes (DEGs) were identified by comparing gene expression levels between September and other months, and multiple genes encoding key enzymes, such as β-AS, and the transcription factors (TFs) that are involved in triterpenoid saponin biosynthesis were further analyzed. The chemical constituents of triterpenoid saponins were identified via ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap mass spectrometry (UHPLC-QE-MS), and triterpenoid saponin content in AF at different developmental periods was determined by high performance liquid chromatography (HPLC). Finally, the expression levels of some unigenes encoding key enzymes were fully verified using quantitative real-time polymerase chain reaction (qRT-PCR). Herein, we elucidate the biosynthetic pathway of triterpenoid saponins in AF and its key enzymes, laying a foundation for future investigations of AF biosynthesis regulation.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"161 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142191235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Approach of Genetic Diversity of Lippia alba (Mill) and Petiveria alliacea L.: Medicinal Plants of Colombia","authors":"Lina Tarazona-Pulido, Paula Andrea Rugeles-Silva, Karen Melissa Cardona Tobar, Lucía Ana Díaz-Ariza, Jaime Eduardo Muñoz Florez, Diana López-Álvarez","doi":"10.1007/s11105-024-01484-z","DOIUrl":"https://doi.org/10.1007/s11105-024-01484-z","url":null,"abstract":"<p>The medicinal <i>Lippia</i> <i>alba</i> and <i>Petiveria alliacea</i>, originating from Central and South America, exhibit a wide range of beneficial properties, including antimicrobial, antifungal, anti-inflammatory, antitumor, analgesic, and antibacterial effects. However, little is known about their population structure and genetic diversity, which may hinder the establishment of their cultivation in different regions of Colombia. In this study, we conducted a comprehensive analysis of the genetic diversity and population structure of 17 samples of <i>L. alba</i> from the departments of Tolima, Valle del Cauca, and Putumayo, as well as 31 samples of <i>P. alliacea</i> from the departments of Cundinamarca, Boyacá, Tolima, and Valle del Cauca. We employed restriction-site associated DNA sequencing (RADseq) with the enzyme PstI. We performed denovo_map and ref_map pipeline for <i>L. alba</i> and identified a total of 17,036 loci and 14,562 SNPs, respectively, revealing a genetic variation of 5.19% (FST of 0.05; <i>p</i> &lt; 0.001) among its populations and two delimited genetic groups. For <i>P. alliacea</i>, in denovo_map our analysis discovered 6395 SNPs, indicating substantial genetic variation of 75% among the studied populations (FST = 0.75; <i>p</i> &lt; 0.001), resulting in the delineation of four genetic groups. Our findings will contribute to providing valuable molecular data on the populations of these medicinal plants and provide evidence of the genetic flow existing among <i>L. alba</i> populations, while <i>P. alliacea</i> populations are more structured. We also performed a FDIST analysis; for <i>L. alba</i> using the results with reference, we identified 37 SNPs coding for proteins in biological, molecular, and cellular processes; and finally, we highlighted the gene SASPL_104284 involved in metabolic processes.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"123 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142191233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression Characteristics of CsPAE55 in Citrus and Analysis of Its Interacting Protein","authors":"Qing He, Zihao Liu, Xiao He","doi":"10.1007/s11105-024-01493-y","DOIUrl":"https://doi.org/10.1007/s11105-024-01493-y","url":null,"abstract":"<p>Exploring the resistance genes of citrus to Huanglongbing (HLB) is the foundation and key to disease resistance breeding. Based on comparative genomic transcriptome data, four pectin acetylesterase (PAE) genes responsive to <i>Candidatus</i> liberibacter asiaticus (<i>Ca</i>Las) infection induction were screened, and a gene cloned with higher differential expression levels was identified, named <i>CsPAE55</i>. Bioinformatics analyses showed that <i>CsPAE55</i> was conserved but had sequence differences compared with homologs. The subcellular localization results of tobacco indicated that CsPAE55 protein was mainly localized in the nucleus and cytoplasm. RT-qPCR analysis showed that the expression of <i>CsPAE55</i> was related to variety tolerance, tissue site, and symptom development. In addition, we established virus vector-mediated infection systems in citrus, namely gene silencing systems mediated by virus-induced gene silencing (VIGS) and gene overexpression systems mediated by citrus leaf blotch virus (CLBV), and obtained <i>CsPAE55</i> silencing and overexpression plants. And we established a stable transformation system mediated by <i>Agrobacterium rhizogenes</i> in citrus and obtained <i>CsPAE55</i> silencing and overexpression citrus hairy roots. The analysis of hormone content and gene expression in <i>CsPAE55</i> plants also indicated that overexpression of <i>CsPAE55</i> regulated the transcriptional regulation of genes involved in systemic acquired resistance (SAR) response. Using Protein–Protein Interaction (PPI) to predict and screen for a citrus protein CsARF1 that may interact with CsPAE55, and preliminarily verifying its interaction with CsPAE55 protein through Yeast Two-hybrid (Y2H) and Bimolecular Fluorescent Complimentary (BIFC). In summary, our data provided theoretical basis and genetic resources for subsequent molecular breeding of citrus resistance to HLB disease.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"4 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142191236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Transcriptome Analysis of Resistant and Susceptible Genotypes of Isabgol (Plantago ovata) During Interactions with Peronospora plantaginis, the Causal Agent of Downy Mildew Disease","authors":"Manivel Ponnuchamy, Sandip Patel, Jincy Mathew, Jitendra Kumar, Nagaraja Reddy Rama Reddy","doi":"10.1007/s11105-024-01491-0","DOIUrl":"https://doi.org/10.1007/s11105-024-01491-0","url":null,"abstract":"<p>Downy mildew (DM) disease, caused by obligate oomycete <i>Peronospora plantaginis</i> Underwood, is the single most damaging disease affecting the yield and quality of isabgol (<i>Plantago ovata</i> Forsk). The infection leads to characteristic ash-colored downy growth, and as the disease progresses, leaf tissue turns yellowish due to loss of chlorophyll, ultimately reducing the effective photosynthetic area. However, reports on the molecular mechanisms mediating host plant resistance are still unknown. In the present study, we conducted a comparative transcriptome between the resistant and susceptible genotypes of isabgol in response to DM infection. We identified significantly differentially expressed genes (DEGs) genes: 6928 in control (RU) vs. infected (RI) of DPO-185 (resistant DM) and 8779 in control (SU) vs. infected (SI) of DPO-14 (susceptible to DM). Putative genes encoding pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), effector-triggered immunity (ETI), cell wall degrading enzymes, phytohormone signalling, and phenylpropanoid biosynthesis pathways involved in host–pathogen interaction were identified in addition to the identification of 58 candidate resistance (R) genes enriched in response to DM infection. The expression of 11 genes involved in plant defense quantified using RT-qPCR indicated a significant difference in the expression pattern. This study for the first time provides a glimpse of transcriptional responses to the DM resistance in isabgol which can guide investigating the molecular mechanisms associated with plant defense and to develop control mechanisms accordingly for DM disease.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"6 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142191241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}