Plant Molecular Biology Reporter最新文献

{"title":"Genetic Diversity and Relationship of Some Sugar Beet Population and Their Correlation with Morpho-physiological Traits","authors":"Maryam Golabadi, Abouzar Asadi, Mojgan Dabaghi","doi":"10.1007/s11105-024-01478-x","DOIUrl":"https://doi.org/10.1007/s11105-024-01478-x","url":null,"abstract":"<p>The present study focuses on the identification of variation in genetic diversity in some of the breeding populations using yield, yield component, and root morpho-physiological traits in <i>Beta vulgaris</i> L. Eighty genotypes from 8 different sugar beet populations were used under a randomized complete block design with five replicates at the Azad University research field during the cropping season of 2021–2022. Significant statistical differences were recorded among the populations that indicate the genetic difference of the studied sugar beet population. Mean comparison of analyzed data revealed, P191 to displayed better traits in terms of sugar content, nitrogen content, pure sugar content, extraction coefficient, and root dry matter. Similarly, population M224 exhibited better root number, root yield, leaf weight, and sugar yield. The dendrogram using UPGMA revealed that the population formed three distinct clusters, with the greatest genetic distance between M249 and the 191 populations. This distance is expected to create maximum heterosis through crossbreeding between these two populations. The results indicate that the number of roots significantly affects RY, LY, Na, and WSY, but does not have a significant correlation with other traits. RY is correlated with LY, Na, and WSY, while LY is inversely correlated with WSC, ECS, and DM, and directly correlated with Na. Principal component analysis based on varimax rotation revealed that three factors explained the total variance to an extent of 87.6%: the first factor named WSC explained 54.5%, the second factor, called RY, accounted for 22.8%, and the third factor contributed to 10.2% of the total variance. The biplot from the first two components showed populations similar to cluster analysis grouping. The findings of this research provide valuable insights into the breeding and improvement of sugar beet populations.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"7 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142191238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DUS-MAS: A Modified Marker-Assisted Backcross Breeding (MABB) Method for Higher Background Genome and DUS Trait Recovery","authors":"Sudip Nandi, Abhijit Kumar Das, Shubhank Dixit, Yathish KR, Ashutosh Sawarkar","doi":"10.1007/s11105-024-01481-2","DOIUrl":"https://doi.org/10.1007/s11105-024-01481-2","url":null,"abstract":"<p>Backcross breeding is used to transfer an desirable trait from donor to recurrent parent (RP), and it takes six to seven generations for 99% recovery of the recurrent parent genome (RPG). Marker-assisted backcross breeding (MABB) was proposed to accelerate the recovery of the RPG. Over the period of time, MABB has evolved as per the available resources and time frame. One of the major modifications is the number of foreground-selected individuals which are subjected to background selection. This number varied widely and lacked consensus in different studies. Further, the major drawback of the existing method is that the DUS traits are recorded directly in the improved lines. Once the traits are fixed in the finished product, there is no going back to bring the corresponding traits of RP. The modified MABB method (DUS-MAS) can ensure higher recovery of DUS traits and RPG. A yield index has also been developed for the identification of high yielding individuals in backcross generation. In DUS-MAS, the foreground-positive individuals are selected first, and then individuals with a higher yield index are selected. Thereafter, the foreground and yield index positive individuals are selected for higher recovery of DUS traits. Five such individuals are subjected to marker-based background recovery of which only two with the highest recovery were advanced to the next generation. All these steps are to be followed in BC₁F₁, BC₂F₁, and BC₂F₂ generations. It substantially reduces the cost by reducing the use of laboratory chemicals by more than 75%.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"2 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142191240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De Novo Transcriptome Assembly and Characterization for Bergenia crassifolia (L.)","authors":"Shiping Cheng, Guomiao Zhao","doi":"10.1007/s11105-024-01483-0","DOIUrl":"https://doi.org/10.1007/s11105-024-01483-0","url":null,"abstract":"<p><i>Bergenia crassifolia</i> (L.) Fritsch, of the family Bergenia Moench, has a broad distribution in China and has been used in Chinese medicine and Chinese gardens extensively. Approximately 544 million high-quality sequence reads and 75,115 unigenes were obtained, among which 40,102 (53.39%) were annotated in NR, 27,769 (36.97%) were annotated in Swiss-Prot, 27,266 (36.30%) were annotated in GO, 12,644 (16.83%) were annotated in KEGG, and 37,808 (50.33%) were annotated in eggNOG database. A total of 1,495 DEUs were obtained, and the expression patterns of these DEUs were gathered into eight categories, and the functions of genes in each categories were analyzed in KEGG pathway. The results may meet the needs for carbon–nitrogen metabolism, adaptation to climate change, and accumulation of secondary metabolites’ studies in <i>B</i>. <i>crassifolia</i> and its relatives.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"7 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142191250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsatellite-Based Genetic Diversity, Population Structure and Bottleneck Analysis in Peanut: Conservation and Utilization Implications","authors":"Chandramohan Sangh, Janki BharatBhai Pandya, Vora Zarna, Radhakrishnan T, S. K. Bera","doi":"10.1007/s11105-024-01492-z","DOIUrl":"https://doi.org/10.1007/s11105-024-01492-z","url":null,"abstract":"<p>Collecting, conserving, and using peanut germplasm depends on our ability to comprehend genetic variability in gene banks. The peanut genetic resources are not well characterized for their conservation and potential future use. Increased use of exotic and interspecific derivatives is required to reduce the yield gap, identify new alleles, and introduce them. We assessed the genetic diversity and organization of an 83-germplasm peanut population. Ninety polymorphic markers generated 326 alleles, and the similarity matrix formed three main clusters. Polymorphism information content (PIC) ranged from 0.09 to 0.85, indicating greater genetic diversity in these genotypes. Germplasm from exotic collections had the highest levels of diversity, followed by released cultivars, and interspecific derivatives had the lowest levels of diversity. The number of private alleles in interspecific derivatives, exotic collections, and cultivars was 1, 6, and 3, respectively. Overall, several SSRs showed a high probability of null alleles. In addition, gene diversity and allelic richness were 0.526 and 3.18, respectively. Analysis of molecular variance revealed variation both within and between individuals. Principal coordinate analysis and population structure analysis subdivided all germplasm lines into three populations. Bottleneck analysis revealed that all three populations experienced genetic bottlenecks, as a shifted mode was observed. This paper highlights the importance of using microsatellite markers to study the diversity of interspecific derivatives, including recently developed ones, and create effective conservation programs.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"7 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142191244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Role of MYB Transcription Factors in Abiotic Stress Responses: An Integrative Approach in Eugenia uniflora L.","authors":"João Pedro Carmo Filgueiras, Thiago Dias da Silveira, Franceli Rodrigues Kulcheski, Andreia Carina Turchetto-Zolet","doi":"10.1007/s11105-024-01489-8","DOIUrl":"https://doi.org/10.1007/s11105-024-01489-8","url":null,"abstract":"<p>MYB transcription factors (TF) play crucial roles in regulating gene expression and orchestrating responses to abiotic and biotic stresses in plants. MYBs were demonstrated to be involved in land plant adaptation. However, little is known about these proteins in native species from heterogeneous environments. <i>Eugenia uniflora</i> (Myrtaceae) is widely distributed in diverse environments within the Atlantic Forest Domain (AFD). In this work, we identified 147 MYB genes in <i>E. uniflora</i> using an integrative approach involving the genome, transcriptome, and phylogenetics analyses. A phylogenetic approach including MYBs from <i>E. uniflora, Eucalyptus grandis</i>, <i>Arabidopsis thaliana</i>, <i>Solanum lycopersicum</i>, <i>Oryza sativa</i>, <i>Vitis vinifera,</i> and <i>Medicago truncatula</i> allowed us to classify the EunMYB in 39 subfamilies already described. The RNA-seq data analysis unveiled the expression patterns of MYB genes under stress conditions in <i>E. uniflora</i> and <i>Eucalyptus grandis</i>. We found 50 EunMYB genes differentially expressed (DE) in <i>E. uniflora</i> under drought stress. In <i>E. grandis</i>, 11 genes were found to be DE under cold and 25 under heat stress. Integrating the expression data, phylogenetic relationships, and the available data from the literature, we highlight the potential MYBs of <i>E. uniflora</i> that may be acting in resistance to abiotic stress, enabling adaptation to diverse environmental conditions.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"46 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141883025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating Molecular Responses to Spittlebug Attack in Paspalum regnellii","authors":"Isabela dos Santos Begnami, Alexandre Hild Aono, Diego da Silva Graciano, Sandra Maria Carmello-Guerreiro, Rebecca Caroline Ulbricht Ferreira, Wilson Malagó, Frederico de Pina Matta, Marcos Rafael Gusmão, Anete Pereira de Souza, Bianca Baccili Zanotto Vigna","doi":"10.1007/s11105-024-01487-w","DOIUrl":"https://doi.org/10.1007/s11105-024-01487-w","url":null,"abstract":"<p>Spittlebugs cause large production losses that affect agribusiness worldwide. Understanding plant-herbivore interactions at the molecular level may be the key to developing resistant cultivars. After a nymph survival experiment, root transcriptomes were assembled from two <i>Paspalum regnellii</i> genotypes (BGP 248 and 344) with different first-line defense strategies, with no infestation and at two times after the initial attack of the spittlebug (<i>Mahanarva spectabilis</i>) nymph, thus integrating differential expression analysis and biological network modeling supplemented by root anatomical analysis. Gene Ontology terms related to different stress responses, such as salicylic acid catabolic process, were enriched in BGP 248, while some specific to spittlebugs, such as response to herbivores, were enriched in BGP 344. Enriched pathways were related to structural differences between genotypes, such as those related to cutin, suberin, and wax biosynthesis. BGP 344 also presented pathways related to induced defense, such as glutathione metabolism. Metabolic networks highlighted kinases, and coexpression networks demonstrated a complex cascade response that included lncRNAs. This study provides the first molecular insights into the defense mechanisms of <i>P. regnellii</i> against <i>M. spectabilis.</i> The genotype with the fastest response to insect attack and highest nymph mortality (BGP 344) presented kinases and an enriched glutathione pathway, in addition to constitutive barriers, such as lignin, which can make it difficult for the insect to colonize the plant.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"108 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141934010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and Molecular Validation of a Lima Bean (Phaseolus lunatus) Core Collection in Brazil","authors":"Rafael da Costa Almeida, Leonardo Castelo Branco Carvalho, Alessandro Alves-Pereira, Josilane Souza da Penha, Verônica Brito da Silva, Maria Imaculada Zucchi, José Baldin Pinheiro, Jaime Martínez-Castillo, Ângela Celis de Almeida Lopes, Regina Lucia Ferreira Gomes","doi":"10.1007/s11105-024-01486-x","DOIUrl":"https://doi.org/10.1007/s11105-024-01486-x","url":null,"abstract":"<p>A gap exists between the growth of active germplasm collections and their effective use because of the size of these collections and the limitations of financial resources, skilled labor, and information on the traits of each accession conserved in germplasm banks. To overcome this, the development of core collections (CCs) is of significant importance. In this study, we established the CCs of lima bean (<i>Phaseolus lunatus</i>) accessions from the Germplasm Bank of <i>Phaseolus</i> at the <i>Universidade Federal do Piauí</i> based on two selection strategies and performed validation based on nuclear microsatellite (ncSSR) data. Two CCs were established from 395 accessions and characterized by 11 phenotypic traits of the seed. These CCs were defined using proportional and logarithmic sampling strategies, each consisting of 79 accessions. A set of 13 ncSSRs was used to validate and estimate the genetic diversity. The estimates of parameters (means, variances, and amplitudes of variation), kurtosis, skewness, and average variability retention index indicated no differences between the two CCs when compared with the entire collection (EC). However, the comparison between the accessions that did not coincide with the two CCs through ncSSRs showed differences. The logarithmic CC showed higher expected heterozygosity, mean number of alleles, and number of private alleles (0.521, 4.308, and 30, respectively) than those of the proportional CC strategy (0.352, 2.154, and 2, respectively), indicating greater genetic diversity. Thus, the logarithmic CC proved to be more suitable for conservation purposes and should be further examined in the establishment of thematic collections of lima beans.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"28 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141863793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Analysis of the SABATH Family in Rosa chinensis and Functional Analysis of RcSABATH20 in Black Spot Disease","authors":"Xiaowen Zheng, Xingyu Liu, Guoqing Han, Haoxiong Yang, Tianchen Yang, Xiaojian Geng, Xiuting Ju, Jie Tian, Tingliang Xu, Nan Tang","doi":"10.1007/s11105-024-01490-1","DOIUrl":"https://doi.org/10.1007/s11105-024-01490-1","url":null,"abstract":"<p>The plant SABATH family of methyltransferases has important biological functions by methylating hormones and signaling molecules and other metabolites. In this study, we identified 47 <i>RcSABATH</i> genes from the <i>Rosa chinensis</i> genome, the majority of which are located in the cytoplasm. Following this, a comprehensive analysis was conducted on <i>SABATH</i> genes of <i>Prunus persica</i>, <i>Malus domestica</i> “Golden,” <i>Prunus avium</i>, and <i>Prunus dulcis</i>, which included phylogenetic and chromosome duplication analysis, gene structure examination, and functional domain and conserved motif analysis. The results revealed that <i>SABATH</i> family members across these five species were divided into three categories, with Group I containing the highest number of family members and Group III the lowest. Gene clusters were observed on the chromosomes of each species, indicating the occurrence of tandem repeat events during the evolutionary process of SABATH members. Furthermore, the analysis of gene structure, conserved motif, and conserved domain highlighted the diversity and conservation among SABATH family members. The transcriptome data of R12–26 and R13–54 infected by <i>Marssonina rosae</i> infection was used to screen 35 <i>RcSABATHs</i>, which were subsequently categorized into three groups (a, b, and c) based on their expression characteristics. Notably, Group c consisted of members that were induced and up-regulated during the late stage of R12–26 infection. Among the identified genes, <i>RcSABATH2</i><i>0</i> drew our attention due to its involvement in various hormone and abiotic stress regulation pathways. To ascertain the function of <i>RcSABATH20</i>, we conducted experiments by treating the leaves of the resistant line R13–54 and susceptible line R12–26 with SA and the SA synthesis inhibitor AIP. The outcomes revealed a direct correlation between the expression of <i>RcSABATH20</i> and resistance to rose black spots. Furthermore, it was observed that SA could enhance the expression of <i>RcSABATH20</i>, thereby contributing to the resistance against rose black spots. These findings establish a theoretical foundation for exploring the molecular mechanisms underlying SABATH resistance to rose black spots.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"21 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141863791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Transcriptomics Reveals the Molecular Mechanisms of Maize in Response to Downy Mildew Disease Caused by Peronosclerospora philippinensis (Weston) Shaw","authors":"Edward Cedrick J. Fernandez, Eliza Vie M. Simon-Ada, Jay-Vee S. Mendoza, Anand Noel C. Manohar, Roanne R. Gardoce, Tonette P. Laude, Fe M. Dela Cueva, Darlon V. Lantican","doi":"10.1007/s11105-024-01477-y","DOIUrl":"https://doi.org/10.1007/s11105-024-01477-y","url":null,"abstract":"<p>Maize is the Philippines’ second most valuable crop based on overall value and total area planted. Still, maize production is hampered by the Philippine downy mildew (DM) disease caused by the pathogen <i>Peronosclerospora philippinensis</i> (Weston) Shaw, causing annual losses in maize production. The use of resistant varieties remains the most effective method of control together with integrated pest and disease management strategies. To date, there are no commercially available maize breeding lines or hybrids deployed in the country while the effectiveness of the fungicide metalaxyl versus DM has declined. Hence, it is necessary to initiate pre-breeding and breeding programs to understand the mechanism of resistance of maize against DM. To comprehend the underlying mechanisms of the presence of the disease, we performed an RNA-Seq comparative transcriptomic approach between mock-inoculated and DM-inoculated susceptible and resistant yellow maize. Among the identified differentially expressed genes (DEGs), we detected 43 DEGs shared in both genotypes which may play roles in the basal defense response of maize upon DM infection. We also identified 68 DEGs exclusive to the susceptible genotype, providing insights into the molecular responses underlying successful DM disease progression in maize. Further, we detected 651 DEGs unique to the resistant genotype. This set of genes revealed that multi-faceted defense strategies govern the molecular basis of DM resistance in maize. These include multi-process regulations such as transcription factors involved in pathogen defense mechanisms, cell wall organization, homeostasis, and many others. Finally, transcriptome-wide variants (SNPs and indels) and their impact on gene function were detected for further application in targeted genotyping-by-sequencing, association studies, and marker-assisted DM resistance breeding.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"64 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141863792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNAi: A Potent Biotechnological Tool for Improvement of Ornamental Crops","authors":"Mukesh Kumar, Veena Chaudhary, Manoj Kumar Yadav, Chetan Chauhan, Ravi Kumar, Deepali Singh, Sachin Teotia","doi":"10.1007/s11105-024-01475-0","DOIUrl":"https://doi.org/10.1007/s11105-024-01475-0","url":null,"abstract":"<p>RNA silencing techniques such as RNA interference (RNAi) and artificial miRNAs (amiRNAs) are important tools for gene silencing. RNAi and amiRNA techniques allow tiny molecules of interfering RNAs to down-regulate the expression of target genes. Both techniques have been used for the functional characterization of several genes across many plant species. In this context, the current review emphasizes upon the uses of gene silencing as a potent technology for improving traits in floriculture crops such as longer vase life, flower shape, flower colour, and plant architecture, including changes in foliage colour. However, the risk assessment of RNAi and amiRNA plants should be looked at to rule out any off-target consequences. Besides, there are other limitations to the effectiveness of this technology, including the choice of target species and crops, the stability of the trigger molecules, and gene candidate selection. Over the past decade, several target genes have been discovered in a variety of flower crops for the improvement of their economic traits. The review highlights the role of gene silencing technology for flower improvement and the long-term sustainable production of floricultural crops.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"16 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141769564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}