RNAi:改良观赏作物的有力生物技术工具

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Mukesh Kumar, Veena Chaudhary, Manoj Kumar Yadav, Chetan Chauhan, Ravi Kumar, Deepali Singh, Sachin Teotia
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引用次数: 0

摘要

RNA 干扰(RNAi)和人工 miRNA(amiRNA)等 RNA 沉默技术是基因沉默的重要工具。RNAi 和 amiRNA 技术允许微小的干扰 RNA 分子下调目标基因的表达。这两种技术已被用于许多植物物种中多个基因的功能表征。在此背景下,本综述强调基因沉默技术作为一种有效技术,可用于改善花卉作物的性状,如延长花瓶寿命、花形、花色和植物结构,包括叶色变化。不过,应关注 RNAi 和 amiRNA 植物的风险评估,以排除任何脱靶后果。此外,这项技术的有效性还受到其他限制,包括目标物种和作物的选择、触发分子的稳定性以及候选基因的选择。在过去的十年中,人们在多种花卉作物中发现了一些用于改善其经济性状的目标基因。综述强调了基因沉默技术在花卉改良和花卉作物长期可持续生产中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

RNAi: A Potent Biotechnological Tool for Improvement of Ornamental Crops

RNAi: A Potent Biotechnological Tool for Improvement of Ornamental Crops

RNA silencing techniques such as RNA interference (RNAi) and artificial miRNAs (amiRNAs) are important tools for gene silencing. RNAi and amiRNA techniques allow tiny molecules of interfering RNAs to down-regulate the expression of target genes. Both techniques have been used for the functional characterization of several genes across many plant species. In this context, the current review emphasizes upon the uses of gene silencing as a potent technology for improving traits in floriculture crops such as longer vase life, flower shape, flower colour, and plant architecture, including changes in foliage colour. However, the risk assessment of RNAi and amiRNA plants should be looked at to rule out any off-target consequences. Besides, there are other limitations to the effectiveness of this technology, including the choice of target species and crops, the stability of the trigger molecules, and gene candidate selection. Over the past decade, several target genes have been discovered in a variety of flower crops for the improvement of their economic traits. The review highlights the role of gene silencing technology for flower improvement and the long-term sustainable production of floricultural crops.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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