{"title":"RNAi:改良观赏作物的有力生物技术工具","authors":"Mukesh Kumar, Veena Chaudhary, Manoj Kumar Yadav, Chetan Chauhan, Ravi Kumar, Deepali Singh, Sachin Teotia","doi":"10.1007/s11105-024-01475-0","DOIUrl":null,"url":null,"abstract":"<p>RNA silencing techniques such as RNA interference (RNAi) and artificial miRNAs (amiRNAs) are important tools for gene silencing. RNAi and amiRNA techniques allow tiny molecules of interfering RNAs to down-regulate the expression of target genes. Both techniques have been used for the functional characterization of several genes across many plant species. In this context, the current review emphasizes upon the uses of gene silencing as a potent technology for improving traits in floriculture crops such as longer vase life, flower shape, flower colour, and plant architecture, including changes in foliage colour. However, the risk assessment of RNAi and amiRNA plants should be looked at to rule out any off-target consequences. Besides, there are other limitations to the effectiveness of this technology, including the choice of target species and crops, the stability of the trigger molecules, and gene candidate selection. Over the past decade, several target genes have been discovered in a variety of flower crops for the improvement of their economic traits. The review highlights the role of gene silencing technology for flower improvement and the long-term sustainable production of floricultural crops.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"16 1","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"RNAi: A Potent Biotechnological Tool for Improvement of Ornamental Crops\",\"authors\":\"Mukesh Kumar, Veena Chaudhary, Manoj Kumar Yadav, Chetan Chauhan, Ravi Kumar, Deepali Singh, Sachin Teotia\",\"doi\":\"10.1007/s11105-024-01475-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>RNA silencing techniques such as RNA interference (RNAi) and artificial miRNAs (amiRNAs) are important tools for gene silencing. RNAi and amiRNA techniques allow tiny molecules of interfering RNAs to down-regulate the expression of target genes. Both techniques have been used for the functional characterization of several genes across many plant species. In this context, the current review emphasizes upon the uses of gene silencing as a potent technology for improving traits in floriculture crops such as longer vase life, flower shape, flower colour, and plant architecture, including changes in foliage colour. However, the risk assessment of RNAi and amiRNA plants should be looked at to rule out any off-target consequences. Besides, there are other limitations to the effectiveness of this technology, including the choice of target species and crops, the stability of the trigger molecules, and gene candidate selection. Over the past decade, several target genes have been discovered in a variety of flower crops for the improvement of their economic traits. The review highlights the role of gene silencing technology for flower improvement and the long-term sustainable production of floricultural crops.</p>\",\"PeriodicalId\":20215,\"journal\":{\"name\":\"Plant Molecular Biology Reporter\",\"volume\":\"16 1\",\"pages\":\"\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-07-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Molecular Biology Reporter\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s11105-024-01475-0\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Molecular Biology Reporter","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11105-024-01475-0","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
RNAi: A Potent Biotechnological Tool for Improvement of Ornamental Crops
RNA silencing techniques such as RNA interference (RNAi) and artificial miRNAs (amiRNAs) are important tools for gene silencing. RNAi and amiRNA techniques allow tiny molecules of interfering RNAs to down-regulate the expression of target genes. Both techniques have been used for the functional characterization of several genes across many plant species. In this context, the current review emphasizes upon the uses of gene silencing as a potent technology for improving traits in floriculture crops such as longer vase life, flower shape, flower colour, and plant architecture, including changes in foliage colour. However, the risk assessment of RNAi and amiRNA plants should be looked at to rule out any off-target consequences. Besides, there are other limitations to the effectiveness of this technology, including the choice of target species and crops, the stability of the trigger molecules, and gene candidate selection. Over the past decade, several target genes have been discovered in a variety of flower crops for the improvement of their economic traits. The review highlights the role of gene silencing technology for flower improvement and the long-term sustainable production of floricultural crops.
期刊介绍:
The scope of the journal of Plant Molecular Biology Reporter has expanded to keep pace with new developments in molecular biology and the broad area of genomics. The journal now solicits papers covering myriad breakthrough technologies and discoveries in molecular biology, genomics, proteomics, metabolomics, and other ‘omics’, as well as bioinformatics.