QTL Mapping and Genetic Map for the Ornamental Sunflower in China

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-12-27 DOI:10.1007/s11105-023-01429-y

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Abstract

Quantitative trait locus (QTL) mapping and genetic map are of great significance for ornamental sunflowers in China. In this study, a total of 956.50 Mbp data were obtained, the average Q30 was 93.76%, the average GC content was 42.43%, and the GC distribution of the parents and F₂ population of the ornamental sunflowers was normal. At the same time, the double-end comparison efficiency of control data was 90.28%, and the enzyme digestion efficiency was 92.01%. The Specific-Locus Amplified Fragment (SLAF) library construction was normal. Furthermore, a total of 734,893 SLAF markers were obtained, among which 127,855 were polymorphic SLAF markers and 38,908 could be used for genetic map construction, and the effective polymorphism of the parents was 5.29%. Moreover, we constructed a total of 17 linkage groups, with 6181 markers in the QTL mapping, the total map distance was 2608.66 cM, the marker integrity in the figure above was 99%, the proportion of double exchange was 0.05, the sequencing depth of the parents was 42.455 × , and the progeny was 9.24 × . The relationship of traits (plant height, stem diameter, disk diameter, number of petals, leaf number, stigma color, petal color, petiole color) and QTL mapping was closely related to show the best of ornamental effect.

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中国观赏向日葵的 QTL 图谱和遗传图谱

摘要数量性状位点（QTL）作图和遗传图谱对中国观赏向日葵具有重要意义。本研究共获得 956.50 Mbp 数据，平均 Q30 为 93.76%，平均 GC 含量为 42.43%，观赏向日葵亲本和 F2 群体的 GC 分布正常。同时，对照数据的双端比对效率为 90.28%，酶切效率为 92.01%。特异性基因扩增片段（SLAF）文库构建正常。此外，共获得 734 893 个 SLAF 标记，其中 127 855 个为多态性 SLAF 标记，38 908 个可用于构建遗传图谱，亲本的有效多态性为 5.29%。此外，我们共构建了 17 个连锁组，在 QTL 图谱中使用了 6181 个标记，总图距为 2608.66 cM，上图中标记完整性为 99%，双交换比例为 0.05，亲本测序深度为 42.455 × ，后代测序深度为 9.24 × 。性状（株高、茎径、花盘径、花瓣数、叶片数、柱头色、花瓣色、叶柄色）与 QTL mapping 的关系密切，显示出最佳的观赏效果。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.