Plant Molecular Biology Reporter最新文献_第7页

Development of EST-SSR Markers and Population Genetic Analysis of Hemsleya zhejiangensis, an Endangered Species Endemic to Eastern China 中国东部特有濒危物种浙江鹤虱的EST-SSR标记的开发与种群遗传分析

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2024-01-20 DOI: 10.1007/s11105-024-01431-y

Zhijian Yan, Shanshan Zhu, Chenxi Wang, Yu Feng, Zupei Lei, Xi Liu, Fangdong Zheng, Weimei Jiang

{"title":"Development of EST-SSR Markers and Population Genetic Analysis of Hemsleya zhejiangensis, an Endangered Species Endemic to Eastern China","authors":"Zhijian Yan, Shanshan Zhu, Chenxi Wang, Yu Feng, Zupei Lei, Xi Liu, Fangdong Zheng, Weimei Jiang","doi":"10.1007/s11105-024-01431-y","DOIUrl":"https://doi.org/10.1007/s11105-024-01431-y","url":null,"abstract":"Hemsleya zhejiangensis C.Z. Zhang (Cucurbitaceae) is a rare and endangered plant species endemic to eastern China and is listed as a key protected species in Zhejiang Province. However, owing to the lack of genomic information and efficient molecular markers, the genetic diversity and population structure of this species remain unknown. In this study, we conducted transcriptome sequencing and de novo assembly of two H. zhejiangensis individuals to develop expressed sequence tag-simple sequence repeat (EST-SSR) markers. Using CandiSSR software, 345 candidate polymorphic EST-SSRs were identified. Twenty EST-SSRs were developed for illustrating the genetic diversity and structure of the four populations of H. zhejiangensis. The populations of ZX (Taishun, Zhejiang) and WYL (Taishun, Zhejiang) have the closest kinship, whereas the populations from WYS (Mount Wuyi, Fujian) showed the farthest kinship with all populations from Zhejiang, suggesting that geographic isolation may significantly impede gene exchange and reduce the genetic diversity of this species. The availability of genomic resource will facilitate subsequent population genetic analyses and molecular breeding, which will be of great significance in formulating strategies for the conservation and utilization of H. zhejiangensis.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"135 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139507955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

QTL Mapping and Genetic Map for the Ornamental Sunflower in China 中国观赏向日葵的 QTL 图谱和遗传图谱

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2023-12-27 DOI: 10.1007/s11105-023-01429-y

{"title":"QTL Mapping and Genetic Map for the Ornamental Sunflower in China","authors":"","doi":"10.1007/s11105-023-01429-y","DOIUrl":"https://doi.org/10.1007/s11105-023-01429-y","url":null,"abstract":"<h3>Abstract</h3> Quantitative trait locus (QTL) mapping and genetic map are of great significance for ornamental sunflowers in China. In this study, a total of 956.50 Mbp data were obtained, the average Q30 was 93.76%, the average GC content was 42.43%, and the GC distribution of the parents and F2 population of the ornamental sunflowers was normal. At the same time, the double-end comparison efficiency of control data was 90.28%, and the enzyme digestion efficiency was 92.01%. The Specific-Locus Amplified Fragment (SLAF) library construction was normal. Furthermore, a total of 734,893 SLAF markers were obtained, among which 127,855 were polymorphic SLAF markers and 38,908 could be used for genetic map construction, and the effective polymorphism of the parents was 5.29%. Moreover, we constructed a total of 17 linkage groups, with 6181 markers in the QTL mapping, the total map distance was 2608.66 cM, the marker integrity in the figure above was 99%, the proportion of double exchange was 0.05, the sequencing depth of the parents was 42.455 × , and the progeny was 9.24 × . The relationship of traits (plant height, stem diameter, disk diameter, number of petals, leaf number, stigma color, petal color, petiole color) and QTL mapping was closely related to show the best of ornamental effect.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"12 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139055081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Traceability of Tobacco C151 Resistance to Potato Virus Y 烟草 C151 对马铃薯病毒 Y 的抗性的可追溯性

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2023-12-14 DOI: 10.1007/s11105-023-01426-1

Aiping Hao, Qiang Zheng, Xin Wen, Ning Liu, Jicheng Wei, Xiuqing Wan, Ruyi Ren

引用次数: 0

Patterns of Diversity of the S and M Loci in Tunisian Apricots (Prunus armeniaca L.): Identification of Pollen-Part Mutations Conferring Self-Compatibility 突尼斯杏（Prunus armeniaca L.）S 和 M 基因位点的多样性模式：鉴定花粉部分突变的自相容性

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2023-12-12 DOI: 10.1007/s11105-023-01425-2

Ayda Boubakri, Hedia Bourguiba, Neila Trifi-Farah, Carlos Romero, Lamia Krichen

{"title":"Patterns of Diversity of the S and M Loci in Tunisian Apricots (Prunus armeniaca L.): Identification of Pollen-Part Mutations Conferring Self-Compatibility","authors":"Ayda Boubakri, Hedia Bourguiba, Neila Trifi-Farah, Carlos Romero, Lamia Krichen","doi":"10.1007/s11105-023-01425-2","DOIUrl":"https://doi.org/10.1007/s11105-023-01425-2","url":null,"abstract":"Self-(in)compatibility (SI) is a genetic barrier developed by flowering plants to prevent self-pollination and promote outcrossing. SI is one of the most important traits in stone fruit breeding and particularly in apricot. Indeed, researchers focused their interests on identifying and selecting self-(in)compatible apricot parents for breeding programs and cultivars for the installation of new plantations. In this context, SI was studied in a set of 65 Tunisian apricot accessions focusing on both S and M loci targeting the genes, S-RNase, SFB, and ParMDO, with different primer combinations. The amplification of the first and the second intron of the S-RNase gene allowed the identification of nine different S alleles. The S7 allele which is reported to be present only in Southern Europe and North Africa regions was the most frequent occurring in 24 genotypes. Moreover, self-pollination tests and amplification of the SFB and ParMDO gene insertions (SC and m haplotypes) producing pollen-part mutations (PPMs) that confer self-compatibility (SC) allowed us to correlate the presence of these two independent PPMs with SC for the first time within Tunisian apricot germplasm. Overall, nine accessions were found to be self-compatible carrying at least one self-compatible haplotype. The distribution of S alleles in the analyzed accessions supported the introduction of apricot to North Africa from the Irano-Caucasian region. Moreover, the detection of the self-compatible ParMDO m haplotype in Kairouan accessions similar to Spanish cultivars confirmed gene flow between Western European and North African apricots. All these results may provide relevant information for apricot breeding and production.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"15 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138580087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome Analysis Reveals Flower Development and the Role of Hormones in Three Flower Types of Grapevine 转录组分析揭示葡萄三种花的发育过程和激素的作用

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2023-12-11 DOI: 10.1007/s11105-023-01424-3

Kai Han, Wei Ji, Yaoxing Luo, Xiaoping Zhang, Naomi Abe-Kanoh, Xiaohe Ma

{"title":"Transcriptome Analysis Reveals Flower Development and the Role of Hormones in Three Flower Types of Grapevine","authors":"Kai Han, Wei Ji, Yaoxing Luo, Xiaoping Zhang, Naomi Abe-Kanoh, Xiaohe Ma","doi":"10.1007/s11105-023-01424-3","DOIUrl":"https://doi.org/10.1007/s11105-023-01424-3","url":null,"abstract":"The molecular mechanism of flower development and sexual differentiation, which is an intricate process, still unclear in Vitis plants. In this study, we performed the transcriptome analysis by high-throughput sequencing to measure gene expression at three stages in male, female, and hermaphrodite grape flowers, and in combination with the fluctuation of hormones (ABA, IAA, BR, GA3, and JA) to understand the gene function and molecular basis of developmental processes in Vitis flowers. The results showed that more than 581 million clean reads were generated from all samples and average 83% reads were mapped on the Vitis vinifera genome. More, quite a large number of genes were differentially expressed between different stages within and across cultivars and various transcription factor families involved were identified in flower development. The genes belonging to MYB, bHLH, ERF, NAC, and WRKY have more numbers than other TF families. Furthermore, we analyzed the correlation of those hormones with 165 DEGs (including 36 TF genes). The results suggested that those genes might play important roles in sex differentiation during flower development and a unique opportunity to increase the efficiency of crossbreeding in Vitis plant.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"69 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138568966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Composition and Catalytic Specificity of Sorghum Histone Acetyltransferase Complexes 探索高粱组蛋白乙酰转移酶复合物的组成和催化特异性

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2023-12-11 DOI: 10.1007/s11105-023-01427-0

Mengli Zhang, Sen Li, Gongjian Zeng, Xiangling Shen, Yongfeng Hu

{"title":"Exploring the Composition and Catalytic Specificity of Sorghum Histone Acetyltransferase Complexes","authors":"Mengli Zhang, Sen Li, Gongjian Zeng, Xiangling Shen, Yongfeng Hu","doi":"10.1007/s11105-023-01427-0","DOIUrl":"https://doi.org/10.1007/s11105-023-01427-0","url":null,"abstract":"Histone acetylation plays important roles in gene regulation and is catalyzed by histone acetyltransferases (HATs). The performance of histone acetylation by HATs requires the involvement of other proteins to form multi-subunit complexes. The functions of three major HAT-containing complexes, namely SAGA, NuA4, and TFIID, have been extensively studied. Recently, the MEDIATOR complex was also found to be associated with HAT in Arabidopsis. However, these complexes have rarely been investigated in other plant species. In this study, we identify the subunits of four HAT-containing complexes in six different grass species by using Arabidopsis homologs as reference sequences. We then construct the phylogenetic trees to analyze their evolutionary relationships. The results indicate that all six grass species conserve all the subunits, despite gene duplication events occurring diversely in different species. Expression analysis shows that most of the genes encoding the subunits of the four HAT-containing complexes are constitutively expressed, with only low-level expression in pollen. Consistent with their cellular function, all four HATs are found to be localized in the nucleus in sorghum. Unlike their Arabidopsis counterparts, SbGCN5 specifically acetylates H3K4, H3K9, and H3K27, while SbMYST acetylates H4K5 and H4K12 in vitro. Additionally, we discover that SbTAF1 catalyzes acetylation of H3K9 and H4K5 for the first time. These findings provide valuable insights for further investigations into the function of HAT-containing complexes in grass species.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"49 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138568528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genes Involved in the Transition and Floral Sexual Differentiation of Jatropha curcas L 参与麻疯树（Jatropha curcas L）过渡和花性分化的基因

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2023-12-09 DOI: 10.1007/s11105-023-01423-4

Adriana Sánchez-Gutiérrez, José Alberto Narváez-Zapata, Miguel Salvador-Figueroa

{"title":"Genes Involved in the Transition and Floral Sexual Differentiation of Jatropha curcas L","authors":"Adriana Sánchez-Gutiérrez, José Alberto Narváez-Zapata, Miguel Salvador-Figueroa","doi":"10.1007/s11105-023-01423-4","DOIUrl":"https://doi.org/10.1007/s11105-023-01423-4","url":null,"abstract":"Flowering is the change from the vegetative state to the reproductive state. Flowering initiation is regulated by endogenous genetic components and by numerous environmental factors. These stimuli determine the different molecular mechanisms that are required to give way to flowering and vary according to the dose of the stimulus and the crop used. Jatropha curcas is a perennial shrub that produces oil from its seeds of adequate quality to be transformed into biofuels. However, this plant still presents limitations for its establishment as a commercial crop since it produces low seeds, which can be attributed to its low production of female flowers. This plant also presents multiple and asynchronous flowering periods as a consequence of the accession and of the climatic conditions, which implies that the fruits do not ripen synchronously, and therefore the harvests are constant, manual, and expensive. Knowing the mechanisms that regulate the flowering of J. curcas is important to improve its seed production. Therefore, current revision covers the analysis of genes involved in flowering to gain information on the pathways involved in floral transition and in the differentiation of the sexual organs of J. curcas.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"28 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138562418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Transcription Factor Gene RDD3 Improves Ammonium and Magnesium Uptake and Accumulation and Drought Tolerance in Rice 转录因子基因 RDD3 可提高水稻对铵和镁的吸收和积累以及抗旱能力

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2023-12-08 DOI: 10.1007/s11105-023-01428-z

Masao Iwamoto

{"title":"The Transcription Factor Gene RDD3 Improves Ammonium and Magnesium Uptake and Accumulation and Drought Tolerance in Rice","authors":"Masao Iwamoto","doi":"10.1007/s11105-023-01428-z","DOIUrl":"https://doi.org/10.1007/s11105-023-01428-z","url":null,"abstract":"We investigated the expression and functions of a transcription factor gene, RDD3. It displays sequence similarity to RDD1, which controls various nutrient ion accumulation and uptake in rice. The RDD3 protein was specifically localized to protoxylems and metaxylems in the vascular bundles. RDD3-overexpressing (RDD3-OX) plants increased the RDD3 protein levels early in the light period and improved NH4+ and Mg2+ uptake and accumulation in the shoots. Furthermore, photosynthetic CO2 assimilation and stomatal conductance in RDD3-OX plants were higher than in wild-type plants, although shoot dry weight was decreased in mature RDD3-OX plants. Subsequent microarray analysis indicated that the late embryogenesis abundant (LEA) protein genes associated with drought responses were upregulated in RDD3-OX plants, whereas WRKY transcription factor genes were downregulated despite participating in defenses against biotic and abiotic stresses. Examining drought stress tolerance indicated that RDD3-OX plants were more tolerant than wild-type plants. These results indicate that increased RDD3 protein levels early in the light period improve nitrogen and magnesium accumulation and drought stress tolerance in RDD3-OX plants.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"165 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138555857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applications of CRISPR/Cas9 Technology in Ornamental Plants CRISPR/Cas9 技术在观赏植物中的应用

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2023-12-07 DOI: 10.1007/s11105-023-01417-2

Hetong Liu, Zhenzhen Wang, Yun Liu, Yamiao Wei, Zongxia Hu, Xiang Wu, Chengshu Zheng, Chengpeng Wang

引用次数: 0

Evaluation of Genetic Diversity and Phylogenetic Relationship among the Major Banana Varieties of North-Eastern India Using ISSR, IRAP, and SCoT markers 利用ISSR、IRAP和SCoT标记评价印度东北部主要香蕉品种的遗传多样性和系统发育关系

IF 2.1 4区生物学

Plant Molecular Biology Reporter Pub Date : 2023-12-02 DOI: 10.1007/s11105-023-01420-7

Saraswathi Marimuthu Somasundaram, Sharmila Gayatri Durairajan, Salini Arumugam Palanivelu, Soundaryan Rajendran, Durai Palani, Chandrasekar Arumugam, Mahendran Jayakumar, Uma Subbaraya

{"title":"Evaluation of Genetic Diversity and Phylogenetic Relationship among the Major Banana Varieties of North-Eastern India Using ISSR, IRAP, and SCoT markers","authors":"Saraswathi Marimuthu Somasundaram, Sharmila Gayatri Durairajan, Salini Arumugam Palanivelu, Soundaryan Rajendran, Durai Palani, Chandrasekar Arumugam, Mahendran Jayakumar, Uma Subbaraya","doi":"10.1007/s11105-023-01420-7","DOIUrl":"https://doi.org/10.1007/s11105-023-01420-7","url":null,"abstract":"India is one of the secondary centers of origin for both wild and domesticated bananas, with the north-eastern region holding the greatest repository. Therefore, the present study used three different molecular marker systems viz., inter simple sequence repeats (ISSR), inter-retrotransposon amplified polymorphism (IRAP), and start codon targeted (SCoT) polymorphism to analyze the genetic diversity among 17 cultivated varieties of the north-eastern region belonging to different genomic groups. The percent polymorphism was found to be 91.79, 86.78, and 82.35 in ISSR, IRAP, and SCoT markers respectively. ISSR had the highest values for all the marker parameters. However, IRAP outperformed SCoT and ISSRs by recording the highest values for effective number of alleles (Ne), Shannon index (I), and Nei’s (1973) gene diversity (H). The dendrogram obtained using ISSR, SCoT, and combined data had two major clusters and the clustering pattern was almost similar, but it differed slightly in IRAP markers. To learn more about the population structure and allelic diversity, model-based structural analysis was carried out in addition to phylogenetic analysis and principal component analysis (PCA). The structure analysis identified three subpopulations in ISSR, four in IRAP, and five in SCoT and combined marker data. The Q-value indicates that almost all the subpopulations are composed of varieties with and without admixture, thereby suggesting that more alleles are being shared among the varieties used in this study.","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"27 27","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138494760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0